LOCUS       DACDYM010000001       120065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDYM010000001 DACDYM010000000
VERSION     DACDYM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 120065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 120065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..120065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.001"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(290..1087)
                     /locus_tag="IWM59_000001"
     CDS             complement(290..1087)
                     /locus_tag="IWM59_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9978071.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1084..2268)
                     /locus_tag="IWM59_000002"
     CDS             complement(1084..2268)
                     /locus_tag="IWM59_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9978072.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2293..3540)
                     /locus_tag="IWM59_000003"
     CDS             complement(2293..3540)
                     /locus_tag="IWM59_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9978073.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3755..5500
                     /gene="dnaX"
                     /locus_tag="IWM59_000004"
     CDS             3755..5500
                     /gene="dnaX"
                     /locus_tag="IWM59_000004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9978074.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5520..5834
                     /locus_tag="IWM59_000005"
     CDS             5520..5834
                     /locus_tag="IWM59_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9978075.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5859..6455
                     /gene="recR"
                     /locus_tag="IWM59_000006"
     CDS             5859..6455
                     /gene="recR"
                     /locus_tag="IWM59_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9978076.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6519..7163
                     /locus_tag="IWM59_000007"
     CDS             6519..7163
                     /locus_tag="IWM59_000007"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9978077.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7178..7507
                     /locus_tag="IWM59_000008"
     CDS             7178..7507
                     /locus_tag="IWM59_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978078.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7521..8459
                     /gene="holB"
                     /locus_tag="IWM59_000009"
     CDS             7521..8459
                     /gene="holB"
                     /locus_tag="IWM59_000009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9978079.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8495..9319
                     /locus_tag="IWM59_000010"
     CDS             8495..9319
                     /locus_tag="IWM59_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9978080.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9312..9659
                     /locus_tag="IWM59_000011"
     CDS             9312..9659
                     /locus_tag="IWM59_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9978081.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9728..10600
                     /gene="rsmI"
                     /locus_tag="IWM59_000012"
     CDS             9728..10600
                     /gene="rsmI"
                     /locus_tag="IWM59_000012"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9978082.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10709..11830
                     /gene="dinB"
                     /locus_tag="IWM59_000013"
     CDS             10709..11830
                     /gene="dinB"
                     /locus_tag="IWM59_000013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9978083.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IWM59_000014"
     CDS             complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IWM59_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9978084.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IWM59_000015"
     CDS             complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IWM59_000015"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9978085.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15316..16083
                     /locus_tag="IWM59_000016"
     CDS             15316..16083
                     /locus_tag="IWM59_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978086.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16073..18115
                     /locus_tag="IWM59_000017"
     CDS             16073..18115
                     /locus_tag="IWM59_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9978087.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18396..18545
                     /locus_tag="IWM59_000018"
     CDS             18396..18545
                     /locus_tag="IWM59_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9978088.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18556..20070
                     /gene="dltA"
                     /locus_tag="IWM59_000019"
     CDS             18556..20070
                     /gene="dltA"
                     /locus_tag="IWM59_000019"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9978089.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20067..21272
                     /gene="dltB"
                     /locus_tag="IWM59_000020"
     CDS             20067..21272
                     /gene="dltB"
                     /locus_tag="IWM59_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9978090.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21317..21550
                     /gene="dltC"
                     /locus_tag="IWM59_000021"
     CDS             21317..21550
                     /gene="dltC"
                     /locus_tag="IWM59_000021"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9978091.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21553..22821
                     /gene="dltD"
                     /locus_tag="IWM59_000022"
     CDS             21553..22821
                     /gene="dltD"
                     /locus_tag="IWM59_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9978092.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22962..24044
                     /locus_tag="IWM59_000023"
     CDS             22962..24044
                     /locus_tag="IWM59_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9978093.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24044..24847
                     /locus_tag="IWM59_000024"
     CDS             24044..24847
                     /locus_tag="IWM59_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9978094.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPMGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24917..25657
                     /locus_tag="IWM59_000025"
     CDS             24917..25657
                     /locus_tag="IWM59_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9978095.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25668..26672
                     /locus_tag="IWM59_000026"
     CDS             25668..26672
                     /locus_tag="IWM59_000026"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9978096.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26717..28561)
                     /locus_tag="IWM59_000027"
     CDS             complement(26717..28561)
                     /locus_tag="IWM59_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978097.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28773..29290
                     /locus_tag="IWM59_000028"
                     /pseudo
     CDS             28773..29290
                     /locus_tag="IWM59_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            29418..30101
                     /locus_tag="IWM59_000029"
     CDS             29418..30101
                     /locus_tag="IWM59_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9978098.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30410..30483
                     /locus_tag="IWM59_000030"
     tRNA            30410..30483
                     /locus_tag="IWM59_000030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30444..30446,aa:Arg,seq:tct)
     gene            30642..31001
                     /locus_tag="IWM59_000031"
     CDS             30642..31001
                     /locus_tag="IWM59_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9978099.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31124..32755
                     /locus_tag="IWM59_000032"
     CDS             31124..32755
                     /locus_tag="IWM59_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9978100.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32817..33458)
                     /locus_tag="IWM59_000033"
     CDS             complement(32817..33458)
                     /locus_tag="IWM59_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9978101.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33740..34048
                     /gene="rplU"
                     /locus_tag="IWM59_000034"
     CDS             33740..34048
                     /gene="rplU"
                     /locus_tag="IWM59_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9978102.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34075..34419
                     /locus_tag="IWM59_000035"
     CDS             34075..34419
                     /locus_tag="IWM59_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9978103.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34432..34725
                     /gene="rpmA"
                     /locus_tag="IWM59_000036"
     CDS             34432..34725
                     /gene="rpmA"
                     /locus_tag="IWM59_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9978104.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35132..37966
                     /locus_tag="IWM59_000037"
     CDS             35132..37966
                     /locus_tag="IWM59_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9978105.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            38158..38649
                     /locus_tag="IWM59_000038"
     CDS             38158..38649
                     /locus_tag="IWM59_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9978106.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38752..39741
                     /gene="manX"
                     /locus_tag="IWM59_000039"
     CDS             38752..39741
                     /gene="manX"
                     /locus_tag="IWM59_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9978107.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39774..40577
                     /locus_tag="IWM59_000040"
     CDS             39774..40577
                     /locus_tag="IWM59_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9978108.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40598..41512
                     /locus_tag="IWM59_000041"
     CDS             40598..41512
                     /locus_tag="IWM59_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9978109.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41595..41969
                     /locus_tag="IWM59_000042"
     CDS             41595..41969
                     /locus_tag="IWM59_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9978110.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42108..42353
                     /locus_tag="IWM59_000043"
     CDS             42108..42353
                     /locus_tag="IWM59_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9978111.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42525..43589
                     /locus_tag="IWM59_000044"
     CDS             42525..43589
                     /locus_tag="IWM59_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9978112.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43835..44260
                     /locus_tag="IWM59_000045"
     CDS             43835..44260
                     /locus_tag="IWM59_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9978113.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44253..44702
                     /gene="nusB"
                     /locus_tag="IWM59_000046"
     CDS             44253..44702
                     /gene="nusB"
                     /locus_tag="IWM59_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9978114.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            44799..45653
                     /locus_tag="IWM59_000047"
     CDS             44799..45653
                     /locus_tag="IWM59_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9978115.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            45654..46991
                     /locus_tag="IWM59_000048"
     CDS             45654..46991
                     /locus_tag="IWM59_000048"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9978116.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            46996..47220
                     /locus_tag="IWM59_000049"
     CDS             46996..47220
                     /locus_tag="IWM59_000049"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9978117.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            47222..48112
                     /locus_tag="IWM59_000050"
     CDS             47222..48112
                     /locus_tag="IWM59_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9978118.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            48121..48939
                     /locus_tag="IWM59_000051"
     CDS             48121..48939
                     /locus_tag="IWM59_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9978119.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            49046..49495
                     /locus_tag="IWM59_000052"
     CDS             49046..49495
                     /locus_tag="IWM59_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9978120.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            49513..51195
                     /gene="recN"
                     /locus_tag="IWM59_000053"
     CDS             49513..51195
                     /gene="recN"
                     /locus_tag="IWM59_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9978121.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(51250..51366)
                     /locus_tag="IWM59_000054"
     CDS             complement(51250..51366)
                     /locus_tag="IWM59_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9978122.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(51439..52395)
                     /locus_tag="IWM59_000055"
     CDS             complement(51439..52395)
                     /locus_tag="IWM59_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9978123.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            52566..52934
                     /locus_tag="IWM59_000056"
     CDS             52566..52934
                     /locus_tag="IWM59_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9978124.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            53104..53535
                     /gene="mraZ"
                     /locus_tag="IWM59_000057"
     CDS             53104..53535
                     /gene="mraZ"
                     /locus_tag="IWM59_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9978125.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            53556..54515
                     /gene="rsmH"
                     /locus_tag="IWM59_000058"
     CDS             53556..54515
                     /gene="rsmH"
                     /locus_tag="IWM59_000058"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9978126.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            54520..54921
                     /gene="ftsL"
                     /locus_tag="IWM59_000059"
     CDS             54520..54921
                     /gene="ftsL"
                     /locus_tag="IWM59_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9978127.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            54922..57114
                     /locus_tag="IWM59_000060"
     CDS             54922..57114
                     /locus_tag="IWM59_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9978128.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            57140..58102
                     /locus_tag="IWM59_000061"
     CDS             57140..58102
                     /locus_tag="IWM59_000061"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9978129.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            58116..59477
                     /locus_tag="IWM59_000062"
     CDS             58116..59477
                     /locus_tag="IWM59_000062"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9978130.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            59503..60591
                     /gene="murG"
                     /locus_tag="IWM59_000063"
     CDS             59503..60591
                     /gene="murG"
                     /locus_tag="IWM59_000063"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9978131.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            60606..61826
                     /locus_tag="IWM59_000064"
     CDS             60606..61826
                     /locus_tag="IWM59_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9978132.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            61982..63307
                     /gene="ftsA"
                     /locus_tag="IWM59_000065"
     CDS             61982..63307
                     /gene="ftsA"
                     /locus_tag="IWM59_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9978133.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            63330..64571
                     /gene="ftsZ"
                     /locus_tag="IWM59_000066"
     CDS             63330..64571
                     /gene="ftsZ"
                     /locus_tag="IWM59_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9978134.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            64588..65265
                     /locus_tag="IWM59_000067"
     CDS             64588..65265
                     /locus_tag="IWM59_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9978135.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            65279..65878
                     /locus_tag="IWM59_000068"
     CDS             65279..65878
                     /locus_tag="IWM59_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9978136.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            65893..66180
                     /locus_tag="IWM59_000069"
     CDS             65893..66180
                     /locus_tag="IWM59_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9978137.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            66229..67011
                     /locus_tag="IWM59_000070"
     CDS             66229..67011
                     /locus_tag="IWM59_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9978138.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            67032..67739
                     /locus_tag="IWM59_000071"
     CDS             67032..67739
                     /locus_tag="IWM59_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9978139.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            68001..70799
                     /gene="ileS"
                     /locus_tag="IWM59_000072"
     CDS             68001..70799
                     /gene="ileS"
                     /locus_tag="IWM59_000072"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9978140.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(70848..72374)
                     /locus_tag="IWM59_000073"
     CDS             complement(70848..72374)
                     /locus_tag="IWM59_000073"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9978141.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(72389..73036)
                     /locus_tag="IWM59_000074"
     CDS             complement(72389..73036)
                     /locus_tag="IWM59_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9978142.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(73220..73549)
                     /locus_tag="IWM59_000075"
     CDS             complement(73220..73549)
                     /locus_tag="IWM59_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9978143.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            73726..74454
                     /gene="sfsA"
                     /locus_tag="IWM59_000076"
     CDS             73726..74454
                     /gene="sfsA"
                     /locus_tag="IWM59_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9978144.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            74470..75483
                     /locus_tag="IWM59_000077"
     CDS             74470..75483
                     /locus_tag="IWM59_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9978145.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            75483..76760
                     /locus_tag="IWM59_000078"
     CDS             75483..76760
                     /locus_tag="IWM59_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9978146.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            76823..79525
                     /locus_tag="IWM59_000079"
     CDS             76823..79525
                     /locus_tag="IWM59_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9978147.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            79677..79994
                     /locus_tag="IWM59_000080"
     CDS             79677..79994
                     /locus_tag="IWM59_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9978148.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            80024..80344
                     /locus_tag="IWM59_000081"
     CDS             80024..80344
                     /locus_tag="IWM59_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9978149.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            80452..81912
                     /locus_tag="IWM59_000082"
     CDS             80452..81912
                     /locus_tag="IWM59_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9978150.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            81980..82201
                     /locus_tag="IWM59_000083"
     CDS             81980..82201
                     /locus_tag="IWM59_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978151.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            82232..82414
                     /locus_tag="IWM59_000084"
     CDS             82232..82414
                     /locus_tag="IWM59_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9978152.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            82414..82827
                     /locus_tag="IWM59_000085"
     CDS             82414..82827
                     /locus_tag="IWM59_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9978153.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            82950..84131
                     /locus_tag="IWM59_000086"
     CDS             82950..84131
                     /locus_tag="IWM59_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9978154.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            84520..84593
                     /locus_tag="IWM59_000087"
     tRNA            84520..84593
                     /locus_tag="IWM59_000087"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84554..84556,aa:Arg,seq:cct)
     gene            complement(84662..85801)
                     /locus_tag="IWM59_000088"
     CDS             complement(84662..85801)
                     /locus_tag="IWM59_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9978155.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(86100..86735)
                     /locus_tag="IWM59_000089"
     CDS             complement(86100..86735)
                     /locus_tag="IWM59_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978156.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(86848..87483)
                     /locus_tag="IWM59_000090"
     CDS             complement(86848..87483)
                     /locus_tag="IWM59_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP9978157.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(87517..87978)
                     /locus_tag="IWM59_000091"
     CDS             complement(87517..87978)
                     /locus_tag="IWM59_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP9978158.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(88108..88539)
                     /locus_tag="IWM59_000092"
     CDS             complement(88108..88539)
                     /locus_tag="IWM59_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9978159.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(88557..88877)
                     /locus_tag="IWM59_000093"
     CDS             complement(88557..88877)
                     /locus_tag="IWM59_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9978160.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            89176..89952
                     /locus_tag="IWM59_000094"
     CDS             89176..89952
                     /locus_tag="IWM59_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9978161.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            89967..90170
                     /locus_tag="IWM59_000095"
     CDS             89967..90170
                     /locus_tag="IWM59_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9978162.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            90186..90524
                     /locus_tag="IWM59_000096"
     CDS             90186..90524
                     /locus_tag="IWM59_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP9978163.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            90511..90690
                     /locus_tag="IWM59_000097"
     CDS             90511..90690
                     /locus_tag="IWM59_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978164.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(90733..91203)
                     /locus_tag="IWM59_000098"
     CDS             complement(90733..91203)
                     /locus_tag="IWM59_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978165.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            91290..91988
                     /locus_tag="IWM59_000099"
     CDS             91290..91988
                     /locus_tag="IWM59_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9978166.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            92166..92507
                     /locus_tag="IWM59_000100"
     CDS             92166..92507
                     /locus_tag="IWM59_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978167.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            92500..93171
                     /locus_tag="IWM59_000101"
     CDS             92500..93171
                     /locus_tag="IWM59_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9978168.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            93177..93863
                     /locus_tag="IWM59_000102"
     CDS             93177..93863
                     /locus_tag="IWM59_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978169.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            93866..94615
                     /locus_tag="IWM59_000103"
     CDS             93866..94615
                     /locus_tag="IWM59_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9978170.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            94627..94896
                     /locus_tag="IWM59_000104"
     CDS             94627..94896
                     /locus_tag="IWM59_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978171.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            95058..95360
                     /locus_tag="IWM59_000105"
     CDS             95058..95360
                     /locus_tag="IWM59_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978172.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            95357..95518
                     /locus_tag="IWM59_000106"
     CDS             95357..95518
                     /locus_tag="IWM59_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9978173.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            95515..95820
                     /locus_tag="IWM59_000107"
     CDS             95515..95820
                     /locus_tag="IWM59_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978174.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            95820..96176
                     /locus_tag="IWM59_000108"
     CDS             95820..96176
                     /locus_tag="IWM59_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP9978175.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            96136..96381
                     /locus_tag="IWM59_000109"
     CDS             96136..96381
                     /locus_tag="IWM59_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978176.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            96378..96797
                     /locus_tag="IWM59_000110"
     CDS             96378..96797
                     /locus_tag="IWM59_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9978177.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            96794..97351
                     /locus_tag="IWM59_000111"
     CDS             96794..97351
                     /locus_tag="IWM59_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9978178.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            97348..97644
                     /locus_tag="IWM59_000112"
     CDS             97348..97644
                     /locus_tag="IWM59_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978179.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            97721..98134
                     /locus_tag="IWM59_000113"
     CDS             97721..98134
                     /locus_tag="IWM59_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9978180.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(98592..98867)
                     /locus_tag="IWM59_000114"
     CDS             complement(98592..98867)
                     /locus_tag="IWM59_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978181.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            99321..99527
                     /locus_tag="IWM59_000115"
     CDS             99321..99527
                     /locus_tag="IWM59_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978182.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            99723..99890
                     /locus_tag="IWM59_000116"
     CDS             99723..99890
                     /locus_tag="IWM59_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9978183.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            99916..100260
                     /locus_tag="IWM59_000117"
     CDS             99916..100260
                     /locus_tag="IWM59_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9978184.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            100265..100546
                     /locus_tag="IWM59_000118"
     CDS             100265..100546
                     /locus_tag="IWM59_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978185.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            100649..100963
                     /locus_tag="IWM59_000119"
     CDS             100649..100963
                     /locus_tag="IWM59_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9978186.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            100941..102635
                     /locus_tag="IWM59_000120"
     CDS             100941..102635
                     /locus_tag="IWM59_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9978187.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            102655..103833
                     /locus_tag="IWM59_000121"
     CDS             102655..103833
                     /locus_tag="IWM59_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9978188.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            103796..104482
                     /locus_tag="IWM59_000122"
     CDS             103796..104482
                     /locus_tag="IWM59_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9978189.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            104482..105642
                     /locus_tag="IWM59_000123"
     CDS             104482..105642
                     /locus_tag="IWM59_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9978190.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            105652..106527
                     /locus_tag="IWM59_000124"
     CDS             105652..106527
                     /locus_tag="IWM59_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978191.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            106524..106835
                     /locus_tag="IWM59_000125"
     CDS             106524..106835
                     /locus_tag="IWM59_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978192.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            106825..107178
                     /locus_tag="IWM59_000126"
     CDS             106825..107178
                     /locus_tag="IWM59_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9978193.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            107168..107569
                     /locus_tag="IWM59_000127"
     CDS             107168..107569
                     /locus_tag="IWM59_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978194.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            107562..107966
                     /locus_tag="IWM59_000128"
     CDS             107562..107966
                     /locus_tag="IWM59_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978195.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            107978..108586
                     /locus_tag="IWM59_000129"
     CDS             107978..108586
                     /locus_tag="IWM59_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9978196.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            108606..108968
                     /locus_tag="IWM59_000130"
     CDS             108606..108968
                     /locus_tag="IWM59_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978197.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            108971..109153
                     /locus_tag="IWM59_000131"
     CDS             108971..109153
                     /locus_tag="IWM59_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978198.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            109170..112601
                     /locus_tag="IWM59_000132"
                     /pseudo
     CDS             109170..112601
                     /locus_tag="IWM59_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            112652..113389
                     /locus_tag="IWM59_000133"
     CDS             112652..113389
                     /locus_tag="IWM59_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9978199.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            113399..115690
                     /locus_tag="IWM59_000134"
     CDS             113399..115690
                     /locus_tag="IWM59_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978200.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            115714..117840
                     /locus_tag="IWM59_000135"
     CDS             115714..117840
                     /locus_tag="IWM59_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978201.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            118003..118449
                     /locus_tag="IWM59_000136"
     CDS             118003..118449
                     /locus_tag="IWM59_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978202.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            118451..118588
                     /locus_tag="IWM59_000137"
     CDS             118451..118588
                     /locus_tag="IWM59_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9978203.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            118626..118919
                     /locus_tag="IWM59_000138"
     CDS             118626..118919
                     /locus_tag="IWM59_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978204.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            118916..119140
                     /locus_tag="IWM59_000139"
     CDS             118916..119140
                     /locus_tag="IWM59_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9978205.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            119137..>120065
                     /locus_tag="IWM59_000140"
     CDS             119137..>120065
                     /locus_tag="IWM59_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9978206.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYV"
BASE COUNT        39965 a        19950 c        25123 g        35027 t
ORIGIN      
        1 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
       61 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
      121 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
      181 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
      241 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
      301 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
      361 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
      421 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
      481 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
      541 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
      601 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
      661 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
      721 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
      781 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
      841 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
      901 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
      961 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
     1021 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
     1081 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
     1141 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
     1201 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
     1261 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
     1321 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
     1381 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
     1441 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
     1501 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
     1561 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
     1621 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
     1681 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
     1741 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
     1801 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
     1861 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
     1921 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
     1981 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
     2041 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
     2101 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
     2161 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
     2221 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
     2281 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
     2341 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
     2401 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
     2461 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
     2521 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
     2581 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
     2641 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg
     2701 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
     2761 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
     2821 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
     2881 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
     2941 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
     3001 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
     3061 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
     3121 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
     3181 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
     3241 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
     3301 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
     3361 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
     3421 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
     3481 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
     3541 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
     3601 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
     3661 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
     3721 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
     3781 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
     3841 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
     3901 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
     3961 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
     4021 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
     4081 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
     4141 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
     4201 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
     4261 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
     4321 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
     4381 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
     4441 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
     4501 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
     4561 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
     4621 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
     4681 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
     4741 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
     4801 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
     4861 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
     4921 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
     4981 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
     5041 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
     5101 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
     5161 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
     5221 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
     5281 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
     5341 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
     5401 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
     5461 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
     5521 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
     5581 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
     5641 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
     5701 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
     5761 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
     5821 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
     5881 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
     5941 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
     6001 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
     6061 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
     6121 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
     6181 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
     6241 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
     6301 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
     6361 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
     6421 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
     6481 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
     6541 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
     6601 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
     6661 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
     6721 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
     6781 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
     6841 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
     6901 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
     6961 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
     7021 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
     7081 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
     7141 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
     7201 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
     7261 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
     7321 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
     7381 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
     7441 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
     7501 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
     7561 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
     7621 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
     7681 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
     7741 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
     7801 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
     7861 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
     7921 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
     7981 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
     8041 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
     8101 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
     8161 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
     8221 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
     8281 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
     8341 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
     8401 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
     8461 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
     8521 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
     8581 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
     8641 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
     8701 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
     8761 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
     8821 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
     8881 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
     8941 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
     9001 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
     9061 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
     9121 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
     9181 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
     9241 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
     9301 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
     9361 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
     9421 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
     9481 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
     9541 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
     9601 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
     9661 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
     9721 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
     9781 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
     9841 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
     9901 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
     9961 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    10021 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    10081 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    10141 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    10201 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    10261 tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    10321 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    10381 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    10441 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    10501 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    10561 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    10621 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    10681 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    10741 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    10801 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    10861 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    10921 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    10981 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    11041 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    11101 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    11161 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    11221 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    11281 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    11341 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    11401 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    11461 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    11521 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    11581 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    11641 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    11701 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    11761 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    11821 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    11881 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    11941 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    12001 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    12061 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    12121 aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    12181 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    12241 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    12301 tggtttgcca tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    12361 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    12421 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    12481 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    12541 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    12601 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    12661 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    12721 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    12781 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    12841 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    12901 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    12961 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    13021 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    13081 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    13141 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    13201 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    13261 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    13321 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    13381 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    13441 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    13501 tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct
    13561 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    13621 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    13681 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    13741 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    13801 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    13861 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    13921 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    13981 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    14041 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    14101 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    14161 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    14221 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    14281 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    14341 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    14401 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    14461 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    14521 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    14581 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    14641 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    14701 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    14761 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca
    14821 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    14881 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    14941 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    15001 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    15061 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    15121 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    15181 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    15241 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    15301 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    15361 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    15421 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    15481 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    15541 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    15601 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    15661 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    15721 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    15781 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    15841 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    15901 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    15961 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    16021 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    16081 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    16141 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    16201 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    16261 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    16321 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    16381 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    16441 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    16501 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    16561 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    16621 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    16681 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    16741 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    16801 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    16861 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    16921 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    16981 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    17041 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    17101 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    17161 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    17221 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    17281 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    17341 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    17401 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    17461 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    17521 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    17581 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    17641 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    17701 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    17761 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac
    17821 agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct
    17881 ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt
    17941 tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta
    18001 tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta
    18061 tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac
    18121 ggaataagac aagataataa atagaactaa attttgaata ccatttattt ggaataagag
    18181 gttgtgacac gatttttgtc acaacctctt tattttttat attttttaaa agtacgagaa
    18241 attataaaga aaaaagcagt gatttgatgt gctattttta taaaagctat gtgacaaaac
    18301 tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc aaaattagtt
    18361 acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt tctaatccaa
    18421 aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca gtactgctgt
    18481 tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc tataacgaat
    18541 tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga ttggggaata
    18601 aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg tgaattgaaa
    18661 caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga tggaccaatt
    18721 gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt tgtcaaatct
    18781 ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct atcgatcttt
    18841 cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga tattccttcg
    18901 gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt aggattttat
    18961 cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc tggtactact
    19021 ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt tcaatggacg
    19081 atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc attttctttt
    19141 gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct gaagccgtta
    19201 aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc cttgaagttg
    19261 aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc tacattcaat
    19321 ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa
    19381 actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac ttacggtcca
    19441 acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact agatagttat
    19501 gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt
    19561 aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag cgtatcaaaa
    19621 ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa tgacttagca
    19681 agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt ttatgaagga
    19741 cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga tatcgaacat
    19801 tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca
    19861 aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt
    19921 cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta tatgattcct
    19981 cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat cgatcgaaaa
    20041 aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct tatgcagagc
    20101 cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct ttattgatat
    20161 gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta tttgtcagct
    20221 ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg tggcagacga
    20281 tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga tggttttttg
    20341 ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct ttttttgaac
    20401 atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt aaatctgtac
    20461 aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt cgttatatcc
    20521 agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat aggagatttg
    20581 aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg
    20641 tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat tttggtcaag
    20701 tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca tgggcattag
    20761 taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc tatagcttat
    20821 ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc aataaacctt
    20881 ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg tccttttggt
    20941 tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa ttttttaaaa
    21001 gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg ggtatatggc
    21061 atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg atctgtctga
    21121 cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct aacaaattta
    21181 cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc ctgatttttt
    21241 caggattttt agataaactt ttttttagct aacgaatagt gacatattaa tttatataac
    21301 aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa taacaggaac
    21361 agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc ttgattcttt
    21421 agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag tccctgtatc
    21481 tgaattcgag cgagaggatt ggagcacacc gaataaaatc attcaacaag caactgcatt
    21541 gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt tttagctgtt
    21601 ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa tccagaaacg
    21661 attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc gataaaaaat
    21721 tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt aagcagaatc
    21781 agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg tccatttttg
    21841 ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc tgcaggagat
    21901 gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt taaaaatggt
    21961 gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga ctggttgttt
    22021 tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt aactttctca
    22081 aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa agggaagcta
    22141 cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa gagagaggat
    22201 gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa
    22261 cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca aattggagaa
    22321 cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca tcgtatccga
    22381 gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt ttcgccagaa
    22441 ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga agtattgttc
    22501 atcatcccac cggttaacga gaaatggagc gattataccg gactatctca agaaatgtta
    22561 caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa tcgaatagct
    22621 gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca tctaggctgg
    22681 aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa tcatattact
    22741 gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca tcaaatacca
    22801 gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt
    22861 ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa
    22921 tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca aaagaagaac
    22981 agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag
    23041 aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac ggtttaggca
    23101 gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct ggacatatgg
    23161 acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa ttccaaactc
    23221 ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag
    23281 ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca cctcaagtaa
    23341 gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca agccaagaag
    23401 aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt gaatacaaac
    23461 gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc gggacagctg
    23521 tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt ttattcgcag
    23581 gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa
    23641 atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca ggaatgacac
    23701 caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt gatgcatcta
    23761 tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa atggatattc
    23821 cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg catttgacaa
    23881 gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat tcccatacat
    23941 cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa ttagtgaaac
    24001 ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata atagaatacc
    24061 tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg gaatcaatac
    24121 ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt ttgacggttt
    24181 cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag tcgataattt
    24241 acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg tacggggtgg
    24301 cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg gttcgatact
    24361 tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa tctttctgcc
    24421 catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt caaatgcggt
    24481 acctatgggt gtcatgccag aagattttct tgatatcagc tttgttttat cgatgatagg
    24541 gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc aatttgctgc
    24601 atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat atggctggtt
    24661 tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta tcggctggta
    24721 tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt
    24781 gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag acgcctattt
    24841 tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag gataaatctt
    24901 tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt atccttataa
    24961 aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt tgatcgatgc
    25021 aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc tagatgctgt
    25081 gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac agtattactg
    25141 gcaacttcat cctaagagaa atccggagac attgctttca atttatggac acacaaaaga
    25201 ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc cttattttga
    25261 agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa tccatccagt
    25321 agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg tatttacagg
    25381 tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc
    25441 agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt caaaagaagc
    25501 tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc tacctcagtt
    25561 tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat
    25621 agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg atttttgtac
    25681 caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg ctgcaagaaa
    25741 tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc atcatcggca
    25801 gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat ggcattcatg
    25861 ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac ctgaacttta
    25921 gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta acacagccaa
    25981 tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc
    26041 tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac ggtcggatgt
    26101 ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat gcattaaaag
    26161 taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata
    26221 tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa
    26281 tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac gaattgactg
    26341 atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat tgggattgga
    26401 attacgggaa gtctcccgat ttcaatttct ctcgccaaaa acgtttttct atcggttcga
    26461 ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt
    26521 tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag aaatatgata
    26581 aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag
    26641 aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa tccacatata
    26701 tggattccgc tattttttat acaggattcg ggtaatttcg agagagttga acagtgatcc
    26761 gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt
    26821 tcatcatcat taagtcaaaa cgttcctgat catctaattc ttctgtgatg cctcgactaa
    26881 ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt
    26941 cgtgtatcga caaataaatt tttcgataca gtggattttc gatggcatga gtttctctaa
    27001 aggaatgctc taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg ataggaagtt
    27061 cttcgggaag atctgcttgg aaggttatct gaatattccg tttggcagct aacttctggt
    27121 aacgcgctaa gataagattt gtcacctggt ggttgcaaaa agaacacagc atttcgaaat
    27181 tttttgcttc ttctaatatc ttttccacat agtttttagc ttttgtttgg tccgtatgca
    27241 ttaaatcttg cagattctcc atctcttgga caaattccag gcgaagtgta tttacttctt
    27301 ggatatgctg tcgaagctgc tcataatgtt gctccaacaa atcatttttg atttttaaga
    27361 aatctacttg tctccgatct ttgtcggtat ttaagatgtg atcgattaga ttgtagctga
    27421 taataacgat tagaatgata atatataaaa tcgtagacca attgatcgtt gtttgataga
    27481 tacctaatgc cgtttggatg taaatcaagc aatggataat ggctaccaag acgatccaag
    27541 gtgtacaagc tacataaaaa cggttattct tatatgcttc gccaatgcat gcaagtgaag
    27601 tgactaaagt gcttataaca ctagcaatat tagcaatcgg cattaattca ggaagttctg
    27661 caggagtaaa agttgcataa agcagaacgc cgataaaaat caaaagttgg aacgtacaaa
    27721 aaattccgta ccatttttta tagtaaaaca ttcggctcca atagtagcag atgatgaaag
    27781 ctgacgttaa gataataaat aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa
    27841 taccacttaa aatatcttct gatacagctt caaatcctaa tgccaaagca aagcaactta
    27901 agagaataag tgagtattca agacgttttt gtttatacat caagtagatc gtatagaaaa
    27961 aggtccctat agcgataaaa acagcgatta tcatcgtcaa tacttgcggc actgattgtg
    28021 aataaataaa actaatcaat ggtccagttg ttcctagata aactttcggt ggcagtccag
    28081 catagttctg ataaggagat gagactgtaa gggtcaattt ttttccgata taatttgctg
    28141 gtaaatctat cacagccaat gagagtccgg gattgctttc ttcctgataa tctttccgtt
    28201 ggtataaaat cttttcgcct agatatacag taatccattg atggtttcct ttgattagta
    28261 aatttgcttc gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat
    28321 ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg
    28381 tatagaaaac ccacgaatct tttatttctt tcatttgatt gctgtccgca ggagaaaaaa
    28441 taaaataaaa gaaataagca attcctgtta aaaataacaa tgataatata atgattatgc
    28501 tccactttat acgtaaataa aaagattctt ttttattata tatgttatta aaaaaagaca
    28561 taaaggtatt cctttctaaa acttgtaatg ttagggtaaa cgttttttaa actaacgtca
    28621 attatttata ataaaaaatg agataaagat gaggaaatag aattagaaac atataattat
    28681 taaagggaaa catttttaca gctataattt gaaaaaaatt tatcgttatt atatgattaa
    28741 gaaggattga ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa ataactttta
    28801 taagttctat aacgttgatt atatttttaa ttcttattct tctcgtcgtc ttggttcccc
    28861 gaatttttgg tttgactcct tatattattg aggacaaaga aatgagtcca tattatgcac
    28921 aaggaaatct gatatatgta cgaccgaaaa atacaaatga gattttagtc ggagatatta
    28981 ttacatatta tgaaaattca ggaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa
    29041 caaccacgaa gatttttata caaaagctga tacaaagact tatattgagc ctggagtagt
    29101 aaaaaagaga aatattatcg gtagtcccat ctttcagatt ccatatattg gactagtcct
    29161 ttcaaaaacg gcatttgcat ggattcaggg gttattcttt acgattgctt tttgcctaac
    29221 aggaatcaca gcatggaata cctttgtaga gttgaaaaag aagcggagac gaacacagaa
    29281 atttttgtaa gaatgttaaa aacataaatg tattccagag atccaacgac tcttctgaac
    29341 atgaaacacg agaaaacact catgcagctt gtagatttag tcagttgttc gtataatgtg
    29401 tgaagaggag gagtagtatg cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac
    29461 tattggattt tatgcgtacc cagcaaaaaa aattaccggg tgagctaggc aaactggaag
    29521 aagaagctca tgtagcagaa gtaccagtta ttccccatga aacagttgta tttttgaaat
    29581 ttttacttgg acagcttcag cctgaacgaa tcttggagat cggagcagct atcggttttt
    29641 catcaagtgt catggccacg acaatcggag aaaacgctca tgttacgaca attgatcgtt
    29701 tcgatgtcat gatcgagaaa gctaaaaaaa actatgaacg tctaggactg acagataaag
    29761 tgactttgct tgaaggtcaa gcagcagata tcttgccgga gttgtctggt ccgtatgact
    29821 ttatttttat ggatagtgca aaatcaaaat atattgaatt tttacctgaa tgcttacgtt
    29881 tgttaagaaa aggtggcgtt ttgatggtag atgacatctt ccaaggagga acgatcttgc
    29941 tcccagatga agagattccc cgtggcaaac gagctatcca tcgtaaatta aatgaatttc
    30001 ttcgtgttgt gatggatcat cccgatttga catcgacaat cttgccatta ggtgatggtg
    30061 tgatcttaat gacaaaggaa tcagaaacaa tcgagttgtg atacatgatt atggcagcct
    30121 gtagataatc gattgaatcg agggctcaat tcgaacttga tgtaaaagtt tctatatttg
    30181 taagtcaatt aaaggataca ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag
    30241 ctcctgtttt caattgataa ccttagaaca gctgaaggag aatgtctcgt tttaaaagat
    30301 tgatttagta gttgggccaa aataattcat tatttgctaa ttaaataact atttcgtatt
    30361 gacagaagca atttaattat ctataattat tgatgttccg ctgattcttg tcatagtagc
    30421 tcagctggat agagcatccg ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg
    30481 acgctaaaaa accgttagaa actgaggttt ctaacggttt ttctttgttt tttgtgccaa
    30541 tattgtttgt tttcaaaggc aaataaaata aaaaaacatc cttatcgagt acaattataa
    30601 ggagcaaaaa aataaaactc atcagaaaag aggaagaaga tatgacgata ctatctagaa
    30661 ttttagtgac tctagttgca attgaatttt tttatatcat gtatatcgaa acggtgagaa
    30721 ctgattctga cacgacaagt agggttttta aaatgagtaa agaagagtta agcagaaaat
    30781 ctgttcaaac attgtttaag aaccaaggag tgtacaatgg actattagga gtgggactac
    30841 tttatggagc ttatctttct tccgcatcta aagaaatcac aagtatgcta ttaatatcta
    30901 ttttttttgt tgcattatat ggcagtttga ctagcgatcg ttggatttta ctcaaacaag
    30961 gtgggctagc tctgttggca ttgctttcta ttttcttata aatatagaaa aaataagaat
    31021 ttatctaaca ggaaactttg tgaaaaatat tactttatct catgaaatat gatattctaa
    31081 tgaatggtat tttagaaaat ttcatgaaaa aggagttaga taggtgttca aaaattatgt
    31141 ttcctcacta aaaaaagaat tttcaggtta tgatctttca agatttcaga aagatctttt
    31201 ggctgggatc acagtagctg ctgtagcatt gcctttagcc ttggcttttg gggtatcaag
    31261 cggagctgat gcggccgcag gtttgattac ggctatcatt gccggtatcg ttattggcgg
    31321 tttatcaggc ggtttttatc aaatatcagg tccgacaggt gcgatggcag ctatcttgat
    31381 gtctattgcg gcaacaaagg gaatgcaagg tgtattgctt gctacttttt tagcgggtct
    31441 atttttactt gcagcaggac ttttccgatt aggaacactg acttctttta tcccagcacc
    31501 cgtaattacg gggtttactt caggtattgc tatcattatt gcattaggtc agatcgataa
    31561 tttatttggt gttcactcgg aaggtacaaa cgttattgaa aaactaagta gttatcaaac
    31621 gataggattt ccgataaaca cagcctcttt gatcatggga gggctagtca ttttagggat
    31681 gtttatctat ccaaaaaaat gggcgaaacg aatgcctagt tcgctgctgg ctatcatttt
    31741 ggcaacagca gttatgctat tgttccattt accagtagca actgtgggta agattccaca
    31801 aacattgatc agtagtaatc gtttgaatat gggagatttt agcttttctg ccttgcaaca
    31861 agtggcagtt ccagctatta gcattgcttt attaggaatg attgaaagct tgctatgtgg
    31921 tgcttcggct ggccgaatgg caaataagcc cttagatagc aatcaagaat taatggcgca
    31981 aggaattgga aatctgttgc ttcctttctt tggcgggatt cctgctactg cggccatcgc
    32041 ccgtacaagc gtggccatca aatctggtgc acaaactaga ttagctggta tgatacatgc
    32101 agttgtctta ctgctctcca tgcttgtttt tgcgccagtg atgtcacaga ttccaatgcc
    32161 agcacttgca ggtgttttga ttgtgacggc ttggcgtatg aatgaatggg aaacgatcaa
    32221 ggagctattt actaataaat attggtcagc agttctattg tttatcctta cgatgatttg
    32281 tacagttatc tttgatttaa gtattgctat cgtgatcggg gtgatcggcg gctgtgtatt
    32341 ttttgttgtc aaaagcgctg ctatcacgat ttcattagaa acgatcgatt ggcaaagaat
    32401 ggggctgcca gaaacggaaa agctgaataa ctggacagta gtgtatatta gtggtccact
    32461 gttttttatg tcttcagaaa aactcaaaaa taaattggaa gaactaaatc atcacgatgg
    32521 tgtcattttc tccatgcgag gcgtaccaag tattgatttg acggcacgtg cagtttttga
    32581 tgaatttcaa gaaaaagcag agcaaagagg acaaacgatt atctatactt ccttgcagcc
    32641 agaagtagaa aaacaacttt ctcacttgtg ggaacataca ttcacgcatc aacatcaaac
    32701 agttgctcat gcgttgacag ctttacatgc gaaatatact gcaattagcc attaaaacaa
    32761 tgaaaaagaa cagacaagac tgtaaactga agattttatg gtcttgtctg ttctttttaa
    32821 tattgctgta aaagtgaagc tagtacttgc cagtcttgat ggataacagg tacaagggag
    32881 cgattgtaga aaatagctgc tggatgaaaa agtggaagaa ttcgatagtt tttttctgac
    32941 cattcgtagg catccttttg ttctgttaat tgtaagacag gccctgtata gagttgacca
    33001 tgatttttgg taaccgtata ttctttgcca agtaaacgtt gaagaccaat gtttccaagt
    33061 gtcgcaatta attttggctg gaccttcatc agttcgtaat ccaatattgg tgcatgcgca
    33121 aatacttcgg aacgagtagg tgtccgattt ggataaacag tttctgactg ttgtgtccgt
    33181 ttattgatcc gatgagtcac gcgatacggg cggctcctta cagcactggt gatatacacc
    33241 tcttctcgtg ttaagccagt tgaagaaagt gcctgcatca attcttttcc agcttgaccg
    33301 ctaaaaggaa catgcgtatc aatctctgtt ttccctgggg cttctcccac gagcatcagt
    33361 ttaggatgaa gaggtccttg cccagcgata aatccttcta caggacggcc gttcattctt
    33421 aaagaagctt cttgtatcag ttcttttggg tattccattt atgctcctcc tttttctttt
    33481 attataagaa taaaagaaac acaatgcgac tattttattt ggtagaactg tgaggataga
    33541 aaagcagaag tattgcttga atttttgttt tactttttga ttttaaattt agttgactaa
    33601 cggttgctgt tctggtaagc tagtactgtt gtaattgtgg actccacagc tacaaccgct
    33661 cagaacaagt aataagcaca gcatatgctg tcacttcgga tggcgagtct aagtatatca
    33721 gaaggaggtg ctttatagca tgtacgcaat tattaaaact ggtggtaaac aagtaaaagt
    33781 tgaagtggga caagcaatct atgttgaaaa acttgacgtt gaagcaggag aaaaagttgt
    33841 ttttgacgaa gttatcttag taggcggaga atctacaaaa gtaggcgctc caactgttgc
    33901 aggtgcaact gttgaaggta ctgttgaaaa acacggcaaa caaaagaaag tcgttacttt
    33961 caaatacaaa cctaaaaaac attcacaccg taaacaaggt catcgtcaac catatacaaa
    34021 agtggttatc gacgcgatca acgcttaata gcgtttgaga aagaaggcct atatatgatc
    34081 aaaggaacat ttaagcgaaa tgacgcaggt cagatcgttt cattcacttt gaccgggcat
    34141 gctgatgctg gtccttatgg aagtgatatc gtttgtgcgg gtgtttctgc gttagcgatc
    34201 agcactgtca acgggattgc ctcattagcc ggttttgagc cgattgttga aatgaatgaa
    34261 gaagaaggcg gttatcttta taccgaagtc acttcaggaa tgacacaaga acaaaacaat
    34321 attgctgaga ttctattaga aaatctttta ttaggattac aatcgataga agctgaaaac
    34381 tcagaatata tccaaattaa aacaattaat gataaatagg aggtgcagac catgttgtta
    34441 tctatgaatc tacaattatt tgcccacaaa aaaggtggcg gttctacatc taacggccgt
    34501 gactcagaat caaaacgtct aggtgctaaa cgtgctgatg gacaaacagt tacaggtgga
    34561 tcaattcttt accgtcaacg cggtacaaaa atctatccag gtgtaaacgt gggtatcggt
    34621 ggagacgaca ctttatttgc aaaagttgat ggcgtagtac gtttcgaacg taaaggccgt
    34681 aacaaaaaac aagtgtctgt ttatccagta gctcaagaag cttaattgaa tcttatgatt
    34741 aaagaggttg tgacaagact tttgtcacag ccttttttgt ttataaaaat aaaagttgta
    34801 gtggaggcta cgaagtttac tttgtctttt tacacttaat atttttttgc caatttggaa
    34861 tcaggatgtt ttgataacta tcgttttcaa ttgatggaag ctatattgtt tgctatcagt
    34921 gaaataatta tatggtaaac agtgttcttt ttcttgtttc cattataaaa atcgtatcgg
    34981 gatttttcgt tcttttaaat tgaacatttc tatctatccg cgtgttatca acaaaacaaa
    35041 atcaggtaaa tagttgacga aacagaaata aatacatagt attatgtttt gaagcctaag
    35101 tacaatagtc tatagaaaag gagcgatacc attggcaaaa cgcattgacc gaatttatat
    35161 gtatgtcaaa gaaaaaaccg ctcatttaac aaaggcagaa tatgatcaag gagtaacaac
    35221 acaggaaatt gctgaagtgt tagggattca gcgaacaaat agcagtaaag atctgaatca
    35281 gctagtgcgc gaaggaaaac tgataaagac agacggacga ccagtacgtt acatatatca
    35341 aacactcgtt acgcacgtaa agccagttac aaaacatgta gtcagttaca aagaaacgcc
    35401 ggtctctgaa gaaaaaaata cactccaaat agatactaag gatatctttg caaaaatcat
    35461 tggagctaac ggcagcatga aaaattcagt ggaacaggca aaagctgcga ttttgtatcc
    35521 gccaagagga ttaaattgtt tgattacagg acctaccgga tcaggaaaga cttattttgc
    35581 acacgcgatg tatcattttg caaaagcgca tcatgtaata gatgaggaaa cggaactgat
    35641 tgttttcaat tgtgctgatt atgccaataa ccctgagctt ttgatgagtc atttatttgg
    35701 gtacgcaaaa ggtgctttta caggcgcaga tgaagaaaaa atggggatta tcgatcaagc
    35761 ggatggcggt atgctgtttt tggatgagat tcaccggttg ccgcctgaag gtcaagaaat
    35821 gatcttttac tttatggatc atggaactta cagtcgtctt ggggaaacaa cgaaatccca
    35881 tgaagcaaat gtacggatcg taggtgcgac cacagaagac ccaggttctt ctctattaga
    35941 aacttttgtt cggcgtatcc caatcaatat caaacttccg gcttttgaaa aacgtcctgc
    36001 aaatgagaaa gtcgatttag taaaaatcat gattgcccac gaagcaaacc gcacgcagcg
    36061 aaagatttct ttgacagaag atgtagcaaa agcattgatc ggcagtgtga cctatggaaa
    36121 tataggacaa ttgaagtcaa atatccagtt agtatgtgca cgaggatttc taaatcatat
    36181 gcaatcaccc gaaatctcga tcacaatcga tgatttgaca gaagggattc gtagcggatt
    36241 gattcaatta gcaagcaacc gcagtgcaat ggcagagctt tctaaaatat tggagccaaa
    36301 gataactgta gcaccaaatg atacgatcat gaaaatccaa tcggactcct atgagttacc
    36361 ttataatcta tatgatatca tcggagataa agcagcttta ctgaagtcgg atggtttgga
    36421 tcaagaagca atcaatcatt tcatttctac agatatcaac atccatctga agtctttcta
    36481 taaagaccat ggtttttctt ttaatgcaga cagcaaatta gcagaatttg tagatccgaa
    36541 aatcatagaa gtgaccaatc agatttatgt aatggtcaaa agatctttac catatgagtt
    36601 tcagcaaaac tttatttatg ctatgagctt gcatatcagt tcttttttga aaaaaatcaa
    36661 cttaggagaa gaacggcata cgaatgataa catccgtgag atggcttttg attttccaga
    36721 tgaatatgct gttgcgaaag aagtgcgtag ttatatcgaa acgtattttc aagtggagat
    36781 tccagaaagt gaagactatt acttgaccgt tctactcgtc tcgttacggg caaatcaagc
    36841 aagcggcaga atcggtgtag tcgttgcagc tcacggaaac agcacagcaa gcagtatggt
    36901 ccaagttgtc caacagttgt tagatgcaga gaacgtacga gcagtagaca tgcctttgga
    36961 tatggatccg aaaacagcat tggaccgaat tgaacgcaat gtacaagaag tagatgaagg
    37021 aagcggggtc atactcttag tagatatggg gtcccttgcg tcgtttaatt cgcaaattca
    37081 aagagacact ggaattccag tacgtactgt tgatatggta acaacttctc tagttttgga
    37141 aactgtacga aaggtctcag tattagggac agatctagat atgttgcatg aatcattgaa
    37201 aaattttcga ggttacgcag aaatatcttc tgtagaatct gttcaggaag caaaacctat
    37261 tcataagaaa aaagctgttt tggctatctg cgcatctggc gaaggtacag cacaaagaat
    37321 caaagaattg atcgaacgag cggtaaacaa gcgtcaagaa acagaattag aagttctagc
    37381 tttgtcgatt gtggaagtga aagaagaact gccagttatc caaaaagaat atcagatcat
    37441 agctactact gggattactg atttgaatat ccaagcccca tttattcctt tagaacgttt
    37501 tattgatcaa aatatcgaag tcattttgga tcagttgctg atggaatcag aattggagga
    37561 aactgaattc atctccctag atgaagagtc agctaaaaat acgtgtgttg agtttatctc
    37621 tgataacttc atctttatta atggtagtaa gttaatcgat ccaatgtggc aattttctac
    37681 acaaatctct cagacgacgg gaatcggcga tgaagaatat ggattcaaaa tcaacctagt
    37741 gatgcacaca gcaggaatga tcgaacgtat tattcggaat gaaccattga ctgtggaaga
    37801 aaacgaattg acaaatacaa cgaatgatcc tctttatagc caattggcag cctcagttgt
    37861 tctgttggaa gatcagataa aagtaaaagt gccgatagaa gaaatgtatt atctgctacg
    37921 tctagtccat aatcagttag ataaaaaaga atacactgtg ccatgaatac acaaaaacga
    37981 tacactttta tagtgtatcg tttttgtgta ttggttaaga atcctttaaa ttcaattagt
    38041 gtatcgtaaa aaaagttggc acgctttttg cttatagtag tgtgtgctag gaggtggttg
    38101 ttcacacaga aatgataggg gtttctgtga agtagtgagg aagaaggagt tttgcagatg
    38161 gatattcgat tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg
    38221 tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa
    38281 ttgagaaagt ttctactaaa agaagctgca ccgccgggga tcacagcaaa tgtaataacc
    38341 gtgcagaaaa tgattcaaat ctatcatagt ggattattat cacgagcaaa agtgatgctt
    38401 ttattcacca atccccaaga tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg
    38461 ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca
    38521 gtaaatcaac aagatattga ttcctttcgt tacttgaaca gtcaagaagt ggagttagag
    38581 attcgaaaag tacctgctga caggaaaatc cttttgatgg aactactagc gaaaatcaaa
    38641 aaggtgtagc aagaatggtt gttggaaaag gtcaaataat tctgttgtaa tggttagcaa
    38701 ttattaatat ttttgaattt gttacaacaa aagtaaatag gaggtataaa tatggtaggg
    38761 attatcctag caagtcatgg acaatttgct gaaggaatct tacaatccgg ttcaatgatc
    38821 tttggcgaac aagaaaacgt caaagccgtg attttgaaac caagtgaagg tccagatgac
    38881 ttgagagcca aattggaaga agcggttgct tcttttgata atcaagacga agtgttattc
    38941 ttagttgatt tatggggcgg aacaccgttc aatcaatcaa acacattatt cgaagagcac
    39001 aaagataaat gggcaatcgt cagcggtttg aatttaccaa tgttgattga agcctacgca
    39061 tcacgctttt caatggaatc tgctcatgaa attgcagcac acatcattga aacagcaaaa
    39121 gacggtgtaa aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa agcagcagtt
    39181 gaagacgcac aacctaaagg agcacttcca gaaggaactg tggtcggaga cggaaaaatc
    39241 aaatatgtat tagcacgtgt tgactcacgt ctgttacatg gacaagttgc gacagcttgg
    39301 acaaaagcag tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc aaaggatgat
    39361 ctgcgtaaga gactaatcga acaagctgct cctccaggtg taaaagcaaa tgttattcct
    39421 attagcaaaa tgatcgaagt agcaaaagac cctcgttttg gaaataccaa agcattgctt
    39481 ttatttgaaa atcctgaaga cgtattgaca gctgttgaag gtggcgtaga catcaaagaa
    39541 ttaaatgttg ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct
    39601 atgggacaag aagacgttga agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat
    39661 gtacgtaaag tacctaatga ttcacgcgat aacatggatg atattttgaa aaaagcaaaa
    39721 gctgaattag caaaagcgta ataaataata ttaaaattta taggaggctt atcatgtcta
    39781 ttatttcaat aattttagtc gtattagttg cctttctagc tggtatggaa ggtattcttg
    39841 atgaatttca atttcatcaa ccactagtgg catgtacatt gatcggttta gttaccggta
    39901 acctcgaagc tggtatcgta cttggcggta cgcttcagat gatcgcttta ggttgggcaa
    39961 atatcggtgc cgctgtagca ccagatgctg cattagcatc agtagcatca gcaattattt
    40021 tagttttagg tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt gccgttcctt
    40081 tggctgttgc agggttgttc ttaacaatga tcgtacgtac aatcgctgtt ccaatcgttc
    40141 atttgatgga tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg tggcacatca
    40201 ttgctgtatg tttacaaggg gttcgtatcg cggtcccagc agcagcgttg ctatttatcc
    40261 cagcacaaac tgtgcaatca ttccttgaat caatgccagc atggttaaca gatggtatgg
    40321 ctatcggtgg gggaatggtc gtagctgtag gttacgcttt ggtaatcaac atgatggcta
    40381 caaaagaagt atggccattc ttcgttattg gtttcgttgt agctgcaatt tctcaattaa
    40441 cattgattgc tctaggtgct ttaggtgttg cattagctct tatttacttg aacctttcta
    40501 aaatgggtgg ttcatcaaat aatggaggcg gaagcaactc tggtgaccct ttaggcgaca
    40561 ttttgaatga ctattaatct tgaaggagga aaataaaatg gcagaagaaa aaatcaaatt
    40621 atcaaaaaaa gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag gttcatggaa
    40681 ctacgaacgt atgcaaaacg gtggctgggc attttctatg attccagcaa tcaaaaaatt
    40741 atacaaaaca aaagaagaac gttctgcagc attaaaacgt cacttggaat tctttaatac
    40801 acacccatac attgcttctc caattttagg ggttacattg gctctggaag aagaacgtgc
    40861 gaatggcgcg cctgttgatg atgtagcgat tcaaggggtt aaagtaggta tgatgggtcc
    40921 tttagctggt gttggtgacc cagtgttctg gtttacagta cgtccaatgc taggtgcact
    40981 aggtgcttca ttagctatgg gaggaaatat ccttggacca atcatcttct tcttaggttg
    41041 gaacattatt cgttgggcat tcatgtggta tacacaagaa tttggctaca aagctggttc
    41101 taaaattact gatgaccttt ctggcggatt gttacaagac gtaacaaaag gtgcttcaat
    41161 tttagggatg ttcgtattag ctgcccttgt acaaagatgg gtatcaatca aattcttacc
    41221 agttgtatca actgtaaaat tagataaagg tgcttacatc gaatgggata aattgccttc
    41281 aggtggtgaa ggaatcaaga gtgcatttga acaagttaat aatggcttag ccctttctcc
    41341 tgaaaaggta acaacattac aagacaactt agaccaattg attcctggtt tagctgcatt
    41401 aggattaaca ttgttatgta tgtggttgct taagaaaaag gtttctccaa tcgttatcat
    41461 ccttggttta ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat aatccataaa
    41521 aatatagctc gggcgttgtc ccgagctttt tttatatagt atactgttca taaacaaata
    41581 tctggaggga gaaaatggtt caatcaatca atacaaaagt agacttggta attgatgcta
    41641 cttcgtttac gggtctgaca gattacgggc aaatcatgat aggtgataat ggttttgagt
    41701 tttaccatgc gcgagataga cgcaaatata tccaaattcc atgggaagaa gtggattatg
    41761 tactagcatc agtcatgcta aagggaaaat ggattcctcg atatgccatt caaacgaaaa
    41821 aaaatggtac atacacattt tcttccaaag aacctaaaaa agtgcttcgt gcgatcagag
    41881 aatatgtaga tgaagataga atggtacaat cattgagttt ctttgatgta gtgaaacgtg
    41941 gtattcgtag tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag aggaggtttt
    42001 ctttattttt ttactttgca gaagaaaata tgaaatagca aaaccaatta tttgtcccct
    42061 ttaaatccca tatgctaaaa ttgattagca aagaaggagg ttatattatg agttggttat
    42121 ggacattgat tgttggagcc gtgatcggag caattgcagg tgcgatcacc agtaaaggaa
    42181 attcgatggg ctgtgtaacg aatatcattg ctggactagt cggttcaagt attggacaag
    42241 cgattttcgg cgaatgggga ccaaaagctg caggtatggc acttattcca tctattttag
    42301 gcgcagtgat tttggtagca gttgtctcat ttttctttgg aaaacgatca taagttttta
    42361 caaaatagta aactaaaagg agttgcagcg agactgtgac tcctttttgg ctttatttat
    42421 ttaaaataaa aagaagtttt ccggaaattt ttatttaact gtcgtgtacg aagaaaagtt
    42481 tgctataatg gaaaaaagcg tgcaaagaaa gagggagttt cattatgatg ctacgtgtag
    42541 aaaaattgcg caaaaaaatg caagaagaaa atttggattc atttttggtt accagtccgt
    42601 acaatcttcg ttacttaacg aactttacag gtacgacagg attagctgtt attactttgg
    42661 aaaaagcatt ttttattact gattttcgtt ataccgagca ggcggcagcg caggctcaag
    42721 ggtttgaaat tattaaaaat gtcggaccta tttttgatga ggtagcaaac ttagtacgca
    42781 aagaagaact gcactcatta gcttttgagg aaacaacagt ctcattttta gaatattcgg
    42841 ttttagaaga aatcatcgat gcggaattag tacctgtttc acagatgatc gaagaactca
    42901 gagaagtgaa agatgaagaa gaaatcgcta ttatcgaaaa agcatgtcat attgcggata
    42961 tggcatatga tcacatcctg aaaatgatcc aaccgggcat gactgagatt gaagtcgcaa
    43021 atcaactaga cttttatatg cgttctcttg gcgcttcagg tgtttctttt gatacgatcg
    43081 ttgctagcgg attgcgttcg gctatgccgc atggtgtagc aagcaaaaaa atcatcgaac
    43141 aaggtgactt gatcacattg gactttggtt gctattacga agggtacgtt tccgatatga
    43201 cacgtacatt cgctattggt gatcctgggg aaaaattaaa agaaatttat caaatagtct
    43261 tagatgctca attagcagtt attgatgctg caaaaccagg aatgactggt gtccaattag
    43321 atgcagttgc tagagattat atttcaaaac atggttacgg ggaagcattt ggacattcta
    43381 caggacatgg gatcggttta gagatccacg aaggaccaaa cgtttcttct cgtgcagaaa
    43441 agcaatttgt accaggaaat gtgatcactg atgaaccagg gatctactta ccaggaatcg
    43501 gtggagtacg tattgaggat gacttgttga tcacagaaga tggaaatcgt gtgttgactc
    43561 gttcaccaaa agaattgatc atcttatagt ttcatcaata gaagaagtaa gcgtaaaaat
    43621 cagtgttcac agactttgtt atagaaaatc actcgattta atgatacact atagaagaac
    43681 ttttaagctg taagagataa taaagttggg tgttttctcc aattcatagc agtttgcagc
    43741 tgatcggcgg cagttttctt accacgtgat cactatgccg catcatagag aattcggatg
    43801 tatctcgtgc ttaggcaaag ggaggaataa ataaatggca gatgaaaaaa atttgatttt
    43861 aaacgcaaac caagatttag gagagatcgt gatcgctcct gaagtcatcg aagtaattat
    43921 cgggattgca gcttctaaag tagaaggcgt atacggaatg cgcggaactt ttgccagcaa
    43981 tgtcactgaa ttattaggac gtgcagctca cggaaaaggt gtttacttag caaacgagga
    44041 agatggcttg aaagtagatc tttactgtta tctagaatat ggtgtttcag ttcctaaagt
    44101 agcaatggaa atgcaggaac gggtcagcca gcaagtcctt ttcatgacgg atatcgattt
    44161 atctgaagtc aatatccatg tagtagctgt tgttcctgaa aaattagaac aaccaacaat
    44221 cgaagaactt tttgaagacg aagaggaaga gaatgaataa agaattatca agacacgaga
    44281 ttcgggaaat ggcattgcag gctttatttc cattagactt caatgcagac ctaacaaaag
    44341 aagatgcgat tttcaacgca atcgaattag accaccgcga catgatcaac gaagatgaat
    44401 cagagtttgt tcctgtttat cttgatacgc tagttggcgg tgtctgtgca aaaaaagatg
    44461 aattggataa agtgattgaa aaacatttaa aaaacaattg gcacatcaat cggatctcaa
    44521 aaatggattt ggtgatcttg cgtatcgcga tttttgaaat gacttatgtg acagatgtac
    44581 cagcggctgt tgctttaaat gaagcaatcg agttggcaaa aacatttagt gatgaccgtt
    44641 cacgtaaatt cgtcaacggt gttttatcca atgtattgaa agaattagaa gctggggcgt
    44701 aagtcctagc ttttttctat gaatcatcat aaacctgtta taaatcggga aaattctatg
    44761 gtagaatgaa aacacaaaaa atatctggga gtggttagat ggcagaattg attaacggaa
    44821 aagaattagc tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata
    44881 atggtatcca tcccggatta gtcgttttat tggtagggga aaatccagcc agccaaatct
    44941 atgtaagaaa taaagaacgt gtagcaaaag aaatcggaat ccattcattg gtagagcgtt
    45001 atcctgagac gatttctgaa gacgaactac ttgcagaaat tgaaaaatac aatcaagatc
    45061 ctagttttca tggtatattg gtccaattac cattgccaaa gcatatagat gaagaaaaga
    45121 tcctgttagc tatccgccct gaaaaagacg tagacggttt tcatccgatg aatttaggca
    45181 gactccttgc gggtaaaccg gacaagattc cttgtactcc atacggcatc atgaaaatgt
    45241 ttgaagctta ttctatcgat ccggcgggta aacgagcagt agttatcgga cgaagcaata
    45301 tcgtcggtaa accgatggct cagctactct tgatggcaga tgcaactgtt actgttgctc
    45361 attctaaaac cgaaaatctg gctgaactgg cacaagaagc agatatttta gtcgtagcaa
    45421 taggacgtgg acattttgtc acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg
    45481 tagggatgaa tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg
    45541 agcctattgc aggctggatc actcctgtgc caaagggtgt agggccaatg actatcacga
    45601 tgctgatgta tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa tagatggcac
    45661 aagaatattt aactgtcact acattaacga agtatttgaa aagaaaattt gatgccgatc
    45721 cttatttaga acgcgtatat ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg
    45781 cccatcaata tttcagctta aaagatgacc atgcaaaaat atctgctatt atgttcaaag
    45841 gagcattcca aaaattacga ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc
    45901 gtatttcatt atatgaagcg ggaggttctt atcaaatcta tgtagagcac atggagcctg
    45961 atggaatagg tgctttatat caagaattgg aagaacgaaa agaaaagctg atgaaggaag
    46021 gcctttttca agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta
    46081 ccagtccaag cggagcggtt atccgcgata tcatcacgac ggtgaaaaga agatatccaa
    46141 ttgtccaatt ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg
    46201 tcaggaatat ccagagagta gaagagatgg gcaatttcga tacaatgatc atcggtcgtg
    46261 ggggtggttc gattgaggat ttatggccgt ttaatgaaga gcgggttgct cgggcgattt
    46321 atcaagctaa tacgccggtg atttcatctg taggacatga aacagatacc acgatcgctg
    46381 atttagtagc agatgttcgg gctgccactc caacagcagc tgcagaatta gctgttcctg
    46441 ttttgtcaga agagttgatg cgcattcaag aacggcaagc tagattgcag caggcgtttg
    46501 tacgccagat ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc
    46561 ggcagcctga aagattgtat gaaggtcaga ccatcaaatt agaccagttg aaacagcgat
    46621 tatatcaagc tacacagcaa atttatcatg cgaaagaaaa acaaacgatc gcattaagcc
    46681 accaattgga acaagtggct ccgctttatc gagttaaagc agctcgccaa gaaactgctt
    46741 atctagaaaa acgcttagaa gagaaaatcc aacaatatat gaaacaacag cagcagcgtt
    46801 ttgctcaagc tgttcaatct cttgatttgt tgagtccatt gaaaatcatg ggaagaggct
    46861 acagctatac gacgttaaat gatcaagtag taaaaagtgt caccgagatt catcaagggg
    46921 atttgctgac gatccattat caagatggaa tcgttaaagg acaagtacaa gagatagaca
    46981 aaggagaata aaaatatgga aaatccaact tttgaagagt cactgcaaga attagaaaaa
    47041 attgtcatgc agttagaaca aggagacgtt ccattagaat cagctctaga ttcgtttaaa
    47101 cgaggaatgg aattaagcaa acattgccaa gatacattag ctaaagcaga agagaccttg
    47161 acgaaaatga tgacagaatc aaatgaagaa gttgattttg acggaaacga ggaaagctga
    47221 gatgagatta actgactttt cagctattca tctacctgct gtagaaaaag aaatcttgtc
    47281 ttttttagat gagcatacaa cggatagagg actctatgat gcgatgagtt attcagtgaa
    47341 agcaggcggg aaaagaatac gtcccttatt acttttaacg gccgttgctt cctttgatga
    47401 gcctattgat gttcccgtct atcaagtagc agctgcttta gaaatggtgc atacgtattc
    47461 cttgatccac gatgatttgc ctgcgatgga caatgatgat cttcgtagag gaaaacctac
    47521 caatcataaa gtattcggcg aagcattagc tatattggcc ggagatggtt tgcttacagg
    47581 agcattccag ctaatcagta tggcacactt aggtaattct cctaagcttc tgctgcttca
    47641 gcaattagct gtttgtgcgg gatcacaagg tatggttgct gggcaagctg cagatatcga
    47701 gggcgaatcg aaaaagcttt ctttagaaga attagcattt attcatgaaa gaaagacagg
    47761 gcatttgatt cgttatgcct tgctggctgg tggcatatta gcgaagcaac ctgaagaaat
    47821 cttattgcta cttcaacgat tagccgaaca tttaggttta gcttttcaga taagagatga
    47881 tctactcgat gtgattggta caacgaaaac attagggaaa acagctggta aagatgaacg
    47941 tatggaaaaa aatacttacc cgcgtttatt aggcctagaa aagacacgtg aggcgttgga
    48001 gattgagctg ttatccgcca acaagatcat agacaaatta gaagaggacg ttttttcttt
    48061 tgatggcggg ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat gagggaaaga
    48121 atggaaaaag aacgcgtaga tgtattagcg gtcaaacaag gattatttga aacaagagaa
    48181 caagccaaac gttcagtgat ggctggtttg atttacaacg aaaaaaacga gcggtttgat
    48241 aaaccgggag aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa aaaacttcct
    48301 tatgtttctc gagggggatt aaaattagaa aaagcactca aagcatttga tctatctgtt
    48361 gaaggaaaaa cattgttaga tattggtgca tccactggcg gatttactga tgcagctttg
    48421 caaaatggcg caaaaatgag ttatgcttta gacgttggct acaatcagct agcttggaaa
    48481 ataagacaag atcctagagt cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc
    48541 gaagacttta ccttaggaat tccagaagtt gctgttatcg atgtttcttt tatttcacta
    48601 aaactgatgc tgcctccatt acatgctatt ttaaaagaag agggagaagt gattgcttta
    48661 atcaaacccc aattcgaagc aggcagagaa gctgtaggga aaaacggaat tgttcgtgac
    48721 cctcagaccc atcaaaaagt tttagaagac attttgtcat ttgcggcaca aggtggatat
    48781 gatgtgctgg aattaagtta ttctccgatc acggggggag aaggaaatat cgagttcttg
    48841 gctcacttga ggaaagtacc cgaaagcgga acaatcaatt cagctattaa tatggcagaa
    48901 gtggtttcaa atgcacacga acagtttgac cataaataaa ttgtaactgt gaaaatcaag
    48961 tgagaagtat gcggattctt ctttattata ccaaggaaat aagctatcat tgattaagaa
    49021 gaatatgcta aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt tgattactcg
    49081 attgttgagt gagcaagata tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa
    49141 aggaatatct gttactcaag ctacgatttc cagagatata aaggaactga aactgatcaa
    49201 ggtaccggct tcagatggcg gctaccgtta tagcatacca gctgaaacaa acgaagatgt
    49261 aaatactaaa ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa
    49321 atttgttatt ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt tgattgataa
    49381 acgttacaag aaagaattat tttcgattat caatgatgac gataatgttt tgatgattac
    49441 acgaactgaa gaccaggcag ttttattgaa gaaagacttc ctgcattacc tttaagttga
    49501 ggagcgaatg tgatgctaca agaaatcagt attactaact ttgcgattat tccggaattg
    49561 cggctttctt ttcacgaagg aatgaccgcg ctgaccggag agactggtgc gggtaaatcc
    49621 attattatcg atgctttagg tctgttagca ggaggaagag gttcaagcga ttatatacgt
    49681 caaggagcag aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca agaaggattc
    49741 tctgaactga tggttgaatt agggatcgaa acagatgagg acaatctgat tgttcgacgg
    49801 gatatgtcct taactggaaa aaatgtttgt cgagtgaatg gtcatattat cacattagct
    49861 aatctgagaa gaatcggcag ttatttagta gatatccaag gacaaaacga acatcaggaa
    49921 ctgctccagc ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt agctttccaa
    49981 cagaagaaaa agagttatca gcaagaatat atctcttatc gtgagctgga aaaaagggta
    50041 cgaaaagtgc agcaaaatga aaaatcctat gttcagagaa tagatatgct ccatttccag
    50101 caagaagaaa tagctgctgc tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa
    50161 agagaaaaac ttagcaatta tcaaaaaatt gctgatggat tagctgcagg ttacggagca
    50221 ctgacgaata atgaacaaaa cagtgtagat ggtgttggct tggccgtttc agaactgcaa
    50281 gggatcgccc atctagatgt ggagtatgaa gcaatttatg aaaatatcca aagtgcttac
    50341 tatttgctgc aagatgcaat cggcgatatg agtcgtcaga ttgatttatt ggagttagat
    50401 gagagtcgtt tagaagaagt tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa
    50461 tacggcgaat ccattgagtc aatattggct tactatgacg aaatcaccga agagctagct
    50521 tcttctgatt tttcagaagg tcaattggac aaaatgaaag aggaactaga gcaaaaagag
    50581 cttctacttc agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa
    50641 ttggaaaaat ccattctgca tgaattgaaa agtctgtata tggaaaacac tgagtttgag
    50701 gtacgtttct taaaagaaga aaatagacaa ttgaatcatg atggatttga ccaaatcgaa
    50761 ttttacatta cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt cgcttctggt
    50821 ggcgaactgt ctcggatgtt gcttgctttg aaaactattt tttcttctga gcaaggtgtg
    50881 acaagtatta tttttgatga agtggataca ggtgtgagcg gaagagttgc acaggccatc
    50941 gcagataaaa tttctcaaat atcgaaatac tctcaagttt tgtgcatcac tcatctgcct
    51001 caagttgctg cagtcgccga ctatcaatat tatattgtga aagcagtgat tgatggacgt
    51061 acgcaaactt ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg
    51121 ctggctggaa gtgagattac aaaactcaca ctcgaacatg caaaagaact attgaaactc
    51181 gcaaaaagga cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat
    51241 attttatttt tacatgacac tcattacagc agtcaataca acagatccaa tcgttaaaat
    51301 cagcattgtc caaatgacaa attttgttac tttagcaaaa gtacttgttt gttttttttc
    51361 acgcattgtt ttcaacctcg tcattaatta gttgattcaa gtgtacaatc ttttttccat
    51421 ttccacaaga gggctttttt ataaaaactt cccttttttt aaataatagg tagttacgat
    51481 acaaataaat aatgttaata gaactagcca tataaaggca tcatcccttt cagcaatagg
    51541 taacttaatg ttcattccat agatactgcc gacgattgtc ggtatagtta aaataatcgt
    51601 taaagaagtt aaaattttca taatattatt cagattattc gatacgattg ctgaaaaagt
    51661 ctgattcatt tgttctacta gttttaattg aatgcgagaa gtagtcattg cctgctctgt
    51721 ttccaccaat acatcacgta aatgattttt caaagcatga ttttcttgga aaagtttatt
    51781 ttgcgctagt tctttcaatg catctaaatt agcagaagtc gcttctctga aataaacgag
    51841 gcttttttgg atatccatga tttgataaag ttgtttgttt tcagtggata cttgaagttt
    51901 ggcttccaaa gtatcgcatt gagattgaag cgtagctaaa taactgttgt aagagagtgt
    51961 gacctgccaa atgaattgaa agaagatatt gagattaggc gaaatatcac tttttggaaa
    52021 ggtctggcta aagactttct ttaaaaacag tggttcatga ttcgtaattg tgataagctt
    52081 ctttttgggc gtcaaaataa tagagagcgg gtatgtatcc acttgaaaat aaccgctagg
    52141 acttgtttta gcaaaaggat attgtaacaa taataacgct ggctttgtca actttgattg
    52201 attgaattct tctgtacggg aattttctgc atcgtccaat atggaagtga tgtaatctga
    52261 cggaatttca aattgatgtg tcagctgatg aatctcttct tgattaggac gttctacaca
    52321 ataccagatc gtttggtcag tttcctttcc ggtattacgc agtttctcat tatctaatgt
    52381 aaaatatttg atcatgattt tcctcctgtt gcctgtttct tttattataa cggataggga
    52441 attggaatac atggaatgtg tttgtaagat atagaatata actgcataaa tttaaataaa
    52501 atgaaacagt tgtgtaaagt atatacagta tacttgaagc aacgataaaa aaggagttta
    52561 cgttcatggg cacattcaca ccacttatct tgttttggta tatttacccg gtaatcgttt
    52621 tgtttggttg ccaatttctt gtaaaattat tttcgttaaa tcgtcgtttt aaaataaaag
    52681 cccctgattt ggcgattcct tttttgtggg gcggtatgca tgcattgtct agaaatacag
    52741 ggacaatttc tatcttaccg tttttcttta tttctgtttt gctaatggga atttgtattg
    52801 ctgttttcca agcatattat tatgaagaaa ttatttatcc gcgttatttc aagatgactt
    52861 ggcgtttggt ttttctgttg acaatcgtct tgtacgctgt cttgatcatt gtgaacatac
    52921 tttcttacct atgaaaaaaa tagaaaagct gtaaaaagtc ttttaaaagg ctttttgcag
    52981 cttgattttt aaaaaaatgt ttatgaataa aaaaagttgt tcccggattt cccagaaaag
    53041 gtggtagaaa gtgggggatt gtggtagact aattttagtt agtggaggaa tggggggctt
    53101 tcaatgttga tgggtgaatt ccaacataat attgatgcaa aaggacgttt aatcgtgccc
    53161 tcaaaacttc gtgaagaact tggcgaaaag tttgttctga cacgcggatt ggatggctgt
    53221 cttttcggct atccgatgag cgaatgggaa aatctggaag ctaaactgaa cgaaatgccg
    53281 ttggctaaaa aagacgcacg tacttttgtg cgattcttct attcggctgc cactgaatgc
    53341 gaacttgata aacaaggacg aatcaatata cccagcacgt taagaaatta tgcagctttg
    53401 acaaaaggtt gcgtagtcat cggtgtatct aaccgaattg aaatttggga tgagacacga
    53461 tggcaagatt tttctgcagc agcagaagag aactttgacg aaattgctga aagtatgatt
    53521 gatttcggac tataagaata ggaagaggct gactaatgac tgaaacattt caacactata
    53581 cagtcatgtt gaaagaaacg gtagatggat tgaacatcaa accggatggc gtttatgtag
    53641 actgtacact cggcggtgcc ggacatagtg aatatttatt aagccaacta agttctaaag
    53701 ggcatctgta tgcgtttgac caagatcaaa aagcaatcga tcacgccaag ctgcgtttag
    53761 cttcttatgt cgaacaaggt atggtcacat ttatcaaagc gaacttcaga gaattagaag
    53821 aacgcttgag tgagcatgta gataaggtag acggcatttt gtatgatcta ggggtttcct
    53881 ctcctcagct agatgaagca gaacgtggat ttagttatca tcaagatgca ccgttggata
    53941 tgcgcatgga tcagtcagct cctttatccg cttatcacgt aattaatgaa tattcctacc
    54001 atgaattagt aaagattttc ttccgctatg gcgaggagaa attttccaaa caaattgctc
    54061 gtgaaatcga acgagtacga aaaacaaaac caatccaaac aactggtgag cttgtcgaaa
    54121 tcattaaaca agtcatccca gctcccgcaa gaagaaaagg cgggcatcct gcaaaaagaa
    54181 tttttcaagc tgtgcggatt gcagtaaatg acgaactcgg tgccgaagag gcctctttag
    54241 aacaggcaat ccggttattg aaagtcaatg gaagaatcag cgtaatcaca ttccattcat
    54301 tggaagacag aattgtcaaa agtatgttta aagaatatag tacggtacaa gatctaccac
    54361 cgggacttcc tgtcatacct gacgaattcc aaccagaact gaaagtcatt actcgtaaac
    54421 caatccttcc ttcagaggaa gagctaacag aaaacaatcg atcacgcagt gcaaagttac
    54481 gtattgcaga aaaaataaga atgaaagagg agtagaatga tggcagaatt aaagaaaatg
    54541 caagattacg aatatgattt acctgaattg gtggatcagc cggaacaaca aatgcctgat
    54601 tcttcttcga atcaagaaat cattcttcca caatcaccgg caaaacgatt aaagcagatt
    54661 tctgctttgg aaaaaattgc tgtggcaagc gtggcgattg ctattgttgc tttatgtgtt
    54721 ttaacagtta tgctgcgcac aaatatcagc ggagctgaaa aagaaatcac gtcaatacaa
    54781 acagaaatca atgataaaaa gcaagaaaaa acgagtttag aacaagaaaa aaacgaactt
    54841 tctcgtactg aacggattaa acaaatcgca gaagaaaaag gattgtctat taatgacgac
    54901 aatttaagga aggtgaagta aatgagtctt aaaaataaaa ttcggcggtt tatggaaaag
    54961 aaaaatctaa atccgatgaa caatcgcaaa aaagtaggta tcattttatt tgctacgagt
    55021 attggattgt tctttttatt tgccgtccgt ttctcgtata tcgttattgg aggtcatgtt
    55081 gctggtacct cattagctga aaaaacaaaa cagttgtacc aaggaagtga agtagtaaaa
    55141 gcaaaaagag gaacgattta tgatcgtaac ggtgtggcgc tcgcggaaga tgcctcctct
    55201 tattcaatta aagcgatact ctcaaaaaca tatacctctg gggataaaaa actgtatgtt
    55261 gaagaaaaga attttgataa aatagcagaa atcctgcata agaatctttc tatagacaaa
    55321 aaagatgcac tgaatatttt agaggacggc gcaaaaaaag agctgtatca agttgaattt
    55381 ggtagctatg ggaaaaatat ctcccaagaa accaagcaaa acatcgaagc ggacatgaaa
    55441 aaagaaggtg tcgctggtct ttattttgtc gaccatcagg ctcggatgta tccaaatggc
    55501 gtcttttctt ctcattttat tggatacgca gttccagaca aagatgagaa tggcttagtt
    55561 ggtaaactag gtttggagtc tgcttacaat gatatcttga gcggtaaaga tggtaagatt
    55621 atttatcaaa aagataattt tcaaaatcct ctgccaggta cagtggcaga agaagaaaaa
    55681 gctgtcgacg gacaagatat ctacacgact ttagacagca gactacaaag ttatttagaa
    55741 acactgatgg atcaagtaaa tgaagaatat cagccagaag aattgacagc agtcctgatg
    55801 aaagcaaaaa ccggagaaat cttggcaatg ggacaacgac ctactttcaa tcctgaaaca
    55861 atggaaggat tgactggaaa agatgctatc tggcgtaatt tcttggtaca agatagttat
    55921 gaaccaggat caaccatgaa agttttcact actgcagctg ctatcgaaga aggagaattc
    55981 aacgagaatg aaaccttcca atctggaaaa atccaagtag aagatgctac gatcaatgac
    56041 catgactttg gtgaaaaagg tgtgctgacc atgcgtcaag ctctttcatg gtcaagtaac
    56101 gtcgggatgg tcatactgga acagcgatta ggcggacgat ggtataacta tttacaaaaa
    56161 ctgggatttg gacaaagtac tcattctgga ttagatgatg aagtaaacgg tgctttacct
    56221 acattaaata ttgtcgaccg agcaatgagt gcttatggac aagcggtagg tgtgacgaat
    56281 ttccaaatga tgaaagcttt cacttctatt gcgaataacg gtacaatgat tcaacctcga
    56341 tatatcagca aagtggtgga tccccaaact ggagaagagc gaacaactca aacagaagtt
    56401 ctagggcaac ctttttcaaa agaaacaaca gaaaaagtcc gtgaatatat gagagatgtt
    56461 gtcgaaagcg aaaactacgg tagtgcttat ggtgtatata gtgtacctgg atacaatgta
    56521 tcggcaaaaa ccggaacagc ccaaatcgct tctgatacag ggggatatca aacaggagat
    56581 acagcctacc tgtattcgat tgtggagatg gttccttctg aagatccaga ctatgtcttg
    56641 tatctaacaa tgaaacaccc taagacatat gatcgaatgg cattggctaa aatcgcaaat
    56701 ccattgatga aacgggcaat ggattttaaa gaaactgaag aagattcaga tacagaaaca
    56761 aaaacagaaa aagtatccgt agccgattat cgtaatcttg aagcggacgt tgcagcagct
    56821 gatgctcaaa aaagtggact gcaacctgta gtgatcggta atggtaaaaa agtacaaaaa
    56881 caatctactg ccaacggtga tcagttgatc tctggagaaa aactgatcct atatactggt
    56941 ggtgataagc tgatgcctga cgtaacagga tggtcaaaag cagatatcat gaagctgggg
    57001 aaaatcctcg gtattgaagt tagttttgat ggagatggtt attgtgtcaa acaagaactt
    57061 gctccatatg aaaaaatcac tgaagacaaa cttagtttta ctttagaaga ataagaagag
    57121 aaataggaga gaaaagagca tggaatggac acaagcattg attcccattg tcagcagttg
    57181 tgcaatgaca attgccgcaa tgccgctatt tattggttat tttcaaatga aaaagcaagg
    57241 tcaggcgatt cgtgaggagg gaccaaagtg gcacaatagc aaagccggaa ctcctacaat
    57301 gggcggctta gtgtttctga tcggctctat tttgacagga atttgggttg gagcttggca
    57361 aaaacagcta actcctactc ttttcatctt gctttttgta ttagcgttat acggagtaat
    57421 cggctttctt gatgatttta taaaaatatt caagaaaaga aacatgggac tgaactcgaa
    57481 gcagaaattg attggacaga ttctaggggg aatcatcttt tatcttgttt accgttctga
    57541 aggttatcca ggtgtattga atttcttcgg actagatcta cctttaggaa tcatttatgg
    57601 tattttcgct ctcttctggc ttgttggatt ttcaaatgca gtaaatctga cagatggcat
    57661 cgatggatta gttgctggct taggaagtat ctcttttgca gcttacggtc taattgcttg
    57721 gcatcagcat cagtatgatg tattaatcat ttgcttaagt gttttaggag gtttactggg
    57781 tttcttcgtc tacaacagaa aacctgccaa gatttttatg ggagatgtcg gttcattagc
    57841 gttgggcgga cttttagcgg caatttctat aatgctaaac caagaatgga cattgctttt
    57901 gattggtttg atctatgtta tggaaacagc aagtgtgatg cttcaagtta cttcctttaa
    57961 gcttacagga aaacgaattt ttaaaatgtc tccaattcat catcactttg aaatgtgcgg
    58021 atggtcggag tggaagattg atacagtttt ttggttgatc agcattgtga cttcgttgat
    58081 cacattatgg atcgtttggt aggagatgac gggaaatgaa aaaaatatca acatatgaaa
    58141 ataagaaagt gcttgtctta ggactcgcaa aaagcggagt aagtgcggcc aaattattac
    58201 atgaattggg cgcacttgtc accgttaatg atggaaaacc ttttgacgaa aatccggaag
    58261 cacaagagct gctttcttta gggataaaag taataacagg tagtcatcca atcgaattgt
    58321 tggatgaaga attttcattg atggtcaaaa atccagggat tccctacagt catcctttag
    58381 tagcaaaagc ccaagaaatg ggtattccgg taattacgga agttgagcta gcttacgaag
    58441 tagcggaatg cccaattatt gggattacag gaacgaacgg aaaaacaacg acaacaacga
    58501 tgacaggact actactgaat gcaggagcag atcaaggaat tgcacgttta gcgggtaata
    58561 ttgggtatcc agcaagtggt gtagcgcaag aagcaaaatc agaagataaa attgtgatgg
    58621 aattgtctag cttccaactc atgggaatca cagatttccg ccctcatata gcggtgatta
    58681 ccaatatcta tgaagcacat attgattatc atggtacaag aaaagaatat gtcaaagcta
    58741 aatggaacct tcaaaaaaac atgacagaaa aagattatct gatccttaac tggaatcagt
    58801 cagaactaca agaattagcg caaagaacaa aagcacgcgt actgcctttc tcgacaaaag
    58861 aagtgttgga agacggtgtg tatgctgacg attactcaat atactataaa aaagaaaaaa
    58921 taatggaaat atctgaactt ggcgtaccag gtaagcataa tgtagaaaat gcactagctg
    58981 ctatttctgt agcaaaattg tatggtatat caaatgaagc tatacgagaa acgttgcatt
    59041 ttttccatgg cgtaccacat cgtacgcaat atgtaggaga aatccaaggc agaaaatttt
    59101 ataatgattc aaaagcaacc aatattttag cgactaaaat ggctctaagt ggatttgaaa
    59161 cgtctaaggt cgtgttattg gctggcggat tagacagagg aaatacattt gatgaattaa
    59221 ttccttcatt aaaaggaatc aaagcaatgg tcgtatttgg ccaaacaaaa gaaaagttga
    59281 tggatgctgg aaaaaaagca ggcatcgaga caatagtaac agccgattct gtagaacaag
    59341 cagttccttt agcattagaa aacagtacag atggtgatgt agttttatta tcaccagcga
    59401 atgccagctg ggatcaatac ccaaattttg aaactagagg aaacagattc atggaagctg
    59461 tgaaccgatt aaaatagaaa aagtagaagc gagcgataaa aaatgaaaat tttggttaca
    59521 ggcggaggaa cagggggaca tatttatcca gctcttgcct ttgtcaatta tgtaaagact
    59581 caagaaccga atgctgaatt catgtatgtg ggagcaaaac gaggtctaga aaacaaaatc
    59641 gtccctgata caggaatgcc tttccatacc ttggagattc aaggatttaa acgaaaaatt
    59701 tccatgcata acatcaagac ggtccgattg tttcttaaga gtatccgtca agcaaaaaaa
    59761 atcctcagtg attttcaacc agatatcgtt atcggtactg gaggatatgt ttccggagcg
    59821 gtagtgtacg ctgcagccaa aatgggtatt ccaacagtta tccatgaaca aaacagtgta
    59881 ccaggtgtta ccaataaatt tttaagccga tatgtggatc ggatcgctct ttcatttaaa
    59941 gatgcggctt ccttttttcc tgaaaacaaa gcagttcttg taggtaatcc tcgagcacaa
    60001 gaagtagcag atactaaaaa atcagatatt ttgaaaacat ttgatttgga tccagaaaag
    60061 aaaacagtct tgatttttgg cggtagtcaa ggagcactta aaatcaatca agcagtatca
    60121 gtttttttaa gtgaatatat tccttcttct taccaagttt tgtatgcttc aggagataga
    60181 tattatgaag agatcctaaa taaaataaat cctgaaatac aaaatattag tgtccagcct
    60241 tatatcaaaa atatggcaga ggtcatggct tccagtgatc tgctggtggg gcgtgctggt
    60301 gcaacttcga tcgcagaatt cacagctctg gggttaccgg cagtgttggt tcccagccca
    60361 tatgttacga atgatcatca gactaaaaat gcaatgagtc ttgttcacgc aggagcagca
    60421 aaaatgattg cagataatga actaacaggt gaatcactaa gtcagacagt caacgaaatc
    60481 atgggtgatg aagaattgca aaagcaaatg tgccgagctt ctaaagaaca aggaatccct
    60541 gatgcttcaa aacgtcttta tgacttagta aaacaaataa tcaaaaaata aaggcggtgg
    60601 tcttagtgag taagaaaaaa gaaccaaaaa aattgtctca tccgtcttct gatgaaaaca
    60661 atttgactcc ttggcaaaag gctaaccgaa aatatctggc agaacaagag atgaaaaacc
    60721 aagagccaaa aaaagaagac ataacattag atacacccct ttcagattct gaagaagaaa
    60781 aaacggttca agaggaacag caagaagaaa gccagtccga gaaaaaaatc gaattttttg
    60841 aagagatcaa gcaaaagaaa aacggtggac catataacgg ttcttttttg aaccggctgc
    60901 ccaatttaaa aagtcaacgg cacaaagtac tatttcgtcg gttagctttg atcatcactg
    60961 tattgggtat accgctgatt tttttgatct attatgtttc tccttatagc aaattacaag
    61021 cagttgcagt atcaggcaat aagacagtca acagccaaga aatcatctct gatacaaaat
    61081 tgtcattagg agaaaatgtt tggggacagt attttcatcg atctacttat atcgatcgcc
    61141 ttaaaaaagc acagccaaga atcgaaacag caaatataca tttcaaaggg atgaatgaat
    61201 tcgatttgga tgtaacagaa tataaagaga ttgctttgat cgcaaaaaac aatcaatact
    61261 acccagtgat tgaaaatgga acagtattag atgaaaaagt agcaaatcct acaaaaaatc
    61321 tccctatctt ggaagagttc aaagactctg caaaaatcaa agaattaaca aagcaataca
    61381 atcagttatc atcagaatta caaaaagcaa tttctgaaat caagtacacg ccaagagcaa
    61441 gcaataaaaa tctgatccaa ttgaatatga atgacggcaa tcaagttatt gtaaatatca
    61501 ataatcttgc gaaccaaatg aaatattatt cgcaagtagc caaagatatg gatgaaaaag
    61561 gtgtgatcga catggaagtt ggtatcttct cgcatcctta cggtagcact caagaaacgg
    61621 aagaatcggg ccaatcggaa gaaacaactg aaagtgaaga aaatgcggat caaacaaacg
    61681 aatcaagtga agaggcagaa acgcaaaaag cacaaacacc tggatcgtct agcacaaacg
    61741 gatcatctga ttcaaatgat tcaagtgaaa caagtcaaat agaagataat ccaagtacaa
    61801 ctccgtcaag tttaaatgaa aattgataaa acaactttaa aaagtaagaa tgtactttct
    61861 aaaaaccagt aatttatggt agaattggaa gcagtgaata tatctattaa taagtatatt
    61921 ttaaaacgga cgctagttgt tcgcttgtat atggaatagt aagtaggagg gaatcccatt
    61981 catggcaaaa acaggaatgt atgtaggcct tgatattgga acgacatctg ttaaagttgt
    62041 cgtggctgaa tatatcgaca gccaaatgaa tattattgga gtaggaaacg caaaatcaga
    62101 agggattaac cgaggcatta tcgttgatat cgataaaacg gtccaagcga tacaacgagc
    62161 agtacgacaa gcagaagaaa aagcaggtat ccagatcaaa ggcgtaagtg tcggtttgcc
    62221 agcaaacatg cttgaagtag aaaattgcca gggaatgatt gcagttaacg gagattcaaa
    62281 ggaaatcacc gatgaagatg tcagaaatgt ggcttcagct gcccttgttc gttcaatacc
    62341 tcctgaacgt caaatcgtgt caatattgcc acaagatttt actgtcgatg ggttcgaggg
    62401 aatcaaagat cctcgtggaa tgatcggcgt aagattagaa atgtacggtc tgttgtttac
    62461 aggacctaaa acgatcatcc ataacatacg taaatgcgtg gaaaatgccg gattaatcgt
    62521 caacgaaatg gtgattactc ctttagcgct gactgaatca attctttctg acggagaaaa
    62581 agattttggt acgatcgtca ttgatatggg tggcggccaa acaacaacgg ctgttatgca
    62641 tgataagcaa ttgaagttta ccaatcttga ccaagaaggt ggcgaatttg tcactaaaga
    62701 tatctctatt gtattaaata cgtcatttaa caatgcagaa gcgttgaaaa taaactacgg
    62761 ggatgcatac ccagaacgga catcaccaga tgaagaattt ccagtagatg tgattggtca
    62821 gtcagaacca gtgaaagtcg atgaacgcta cttgtcagaa attatttcag ctcgaatgga
    62881 acagattttc aacaaagcaa aagatgcatt ggaccaaatc gaagcattag agttgccagg
    62941 tggcgtcgta ttgacaggcg gagctgctag tcttcctggt gttgtggact tggcacaaga
    63001 aatcttcggc gtaaatgtaa aattgtatgt tccaaatcac atggggctga gaaacccggt
    63061 atttacaaat gtaataagta tcgtggatta ttcagcaaac ttgagtgaag tgtatcaatt
    63121 ggcaaaaatt gctgttacgg gtgaaacctc agcagctcgt caaatggtag ttgaacaaga
    63181 aacaacaaac acatatgaga gctatgaagc acctgaagaa acaatctacg atgagccgga
    63241 accaaaagag tccggagaaa atgtaaaaaa taaaatcaag ggtttcttta caaatatttt
    63301 tgattaattc atcctgggag gaataagata tggaattctc aattgataat aatatcaatg
    63361 acggcgcagt aatcaaagtc atcggagtcg gtggtggcgg tggaaatgct gtaaaccgaa
    63421 tgattgaaga aaacgttaaa ggtgttgaat tcattacagc caacacagac gtgcaagcat
    63481 taaaaaattc aaaagctgaa acagttatcc aattaggccc caaatatact cgtggtttag
    63541 gtgctggttc tcagccagaa gttggacaaa aagcggcaga agaaagtgaa caatccttac
    63601 gcgaagcttt agacggtgca gatatgattt tcatcactgc aggaatgggt ggaggaaccg
    63661 gtacaggtgc tgcaccaatc gttgcaggta tcgcgaaaga attgggcgca ttgactgttg
    63721 gtgtcgtgac acgtccattc acttttgaag gaccgaaacg tggccgtttt gctgcagaag
    63781 gtatcgctcg tttgaaagaa aatgtggata cattattaat tatctcaaat aaccgtttat
    63841 tagaagttgt agataaaaag acaccgatgc ttgaagcttt ccgtgaagca gataatgttt
    63901 tacgacaagg tgtacaaggt atctctgatt tgatcacagc gccaggttac gttaaccttg
    63961 actttgctga cgtgaaaaca gtgatggaaa accaaggtac agcattgatg gggatcggtg
    64021 tggctagtgg agaagatcgc gtagtagaag caacgaaaaa agcaatctcc tctccattgc
    64081 ttgaaacatc tattgatgga gctgaacaag tacttcttaa catcacaggc ggtttggata
    64141 tgacattgtt cgaagcacaa gatgcttcag atatcgtagc aaatgcagca actggcgatg
    64201 taaatatcat tctaggtaca tctatcaatg aagaaatggg cgatgaaatc cgtgtgacgg
    64261 tcatcgcaac aggtatcgat gaatcaaaaa aggaacgcaa gtctagccga ccagctagac
    64321 aagcacaaat gcaatcacct gctcaaaaga ctgtattaga tatggatcaa gctaaaccaa
    64381 cttcttcaga agaagaaaac agttttggcg attgggatat cagacgtgaa caaaatgttc
    64441 gtcctagagt tgatgattcc aattttgaca atatcgaaaa aaaagaattt gatacattca
    64501 accgcgaaga aacaaaatct catggcgatg atgaattaaa tacgccaccg tttttccgtc
    64561 gtaaaaaata aaggagagga agagcacatg attgctgaca acttgcagaa aatcaagcaa
    64621 gaaattcagg attcgtgtgc tcttgttcat cgtgatccga aagaagttac attagtagct
    64681 gtaacaaaat ctgttgaatc cgctcaagct gaagaattgg ttagagctgg cgttatgaat
    64741 tgtgcagaaa accgagtaga taaattatta gaaaagaaac aagatttatt tgattttcct
    64801 gaaataaagt ggcatttaat tggtaattta cagcgcagaa aggtaaaatt gattgtaaat
    64861 gaaattgatt actttcatgc gttggacagt ttaagattag cagaagaaat ccagaagcgg
    64921 ttagagaaag aattatcctg ttttattgaa gtaaatgttt ctggtgagga atcaaaacat
    64981 ggaatttcac cagatgagct tttaccgttt gtggaatctt tagctgcctg cacaaaaatc
    65041 aaggttgttg ggttgatgac tatggcgcct aaagaagctt ctgatgagca aatccataaa
    65101 gtgtttggta cgctgaaaga attgcgtgat cttgttcaag caaaaggtta tgcccatgca
    65161 ccatgtagtg aattaagtat ggggatgagt caagactttc aaattgcgat tgaagaaggt
    65221 gcaacattta tccgtgtagg aacagcgcta ttcaaagacg aataaaggag gaacaaaaat
    65281 gccgctttta aacagaggag caattgctaa ttttttcgga ttagctgatg aggaagagta
    65341 cgaataccaa gattatcctg aaacaacaaa aaaggctgta cagccaacta aaaaaaatac
    65401 aacacagaat caatcaactc attctgtatc tgctgcgaat actcatgaaa aacaaaaaac
    65461 ggcgacttca cgtcctgtaa cgcatgaaag tattcagccg gctgaaaaga aaaaagtaat
    65521 tgaactacat caatcaccta caaaaactgt agcgaaagaa agaacagctt caagaacctc
    65581 tacacaacct aaagcaaatc ttgctggaaa aatcactgta atggaaccaa gagcttattc
    65641 tgaagctatg gcgattgcta aacgaatcat tgctggagaa gcaaccttag tgaattttca
    65701 tttagtagaa gaaaaacaag cgagaagaat cgttgatttt ctaacaggaa cagtttatgc
    65761 actagatgga gatatcaaac gtgttggaga tgagattttt ctttgtacac cgactgggat
    65821 ggagattgac agttcaaccg ctcaatcttt tgcagatgcg aatttgtttg atctgtaaga
    65881 ggaggaacta cgatttatac tattgcacta ttacttcatt tacttcaacg ggcagcctat
    65941 ttctatagca ttatcttgat ggtatatgca ttgctctctt ggtttccagg cgggtatcaa
    66001 tcagggctag gacgattatt agctaagatc tgcgaaccgt atatcagttt gttcgatcgt
    66061 ctgcctttga ggattggacc cttggatttc acaattttag gggcaatcct gatcttgaac
    66121 ttagccgtac aaggtttagc acagatcgtt atatggataa ctgtccattt agtttattga
    66181 ttgaagcaac aaaatcttta ttctcagaat agataggagg ggaaaatttt ggatgcaaat
    66241 gtataccagc actttcgggc agatgaacgt acatttatcg attcggtagg cgattggatt
    66301 gaacaggttc aaggacaata tgcaccctat ctgaccgaat ttcttgaccc aagacaatcc
    66361 tatattttag aaacactgat tcgacaaagc agtgatttgc gtttcatgtt ttatggcgga
    66421 tatgaacagg cagaaagaaa aagatgtttg attttccccg attattacga acctgtagag
    66481 gaagattttg aaacagcatt gtatgaaatc cattatccta gcaaatttgc cagcctaagt
    66541 catgggaaaa tccttggaac tttagtggga acggggatca aacgctctta ctttggcgat
    66601 atcatctcgg acggtgatcg ctggcaagta tttattgcaa atgaggtgga tcattttgtt
    66661 gctgcacaag taacaaagat tggaaaggtt gctgtacgct tggaaccaat caaatacaca
    66721 gaaatgatta tgccgaaaga tagctggaca caagaaagag gaacagttag ttccatgcga
    66781 ctagacagtg tgatctctga gatttataat atttccagac agcggtccaa gcagctgatc
    66841 gaagctggta aagtaaaagt gaactggaca gaaaatacaa gacctgattt tgtacttgaa
    66901 ctattagata ttatttcagt cagaggttat ggcagagtcc agatccaaga acttgagggg
    66961 aagacgaaaa aagaaaagta cagggtactg ttcggtgtat tacgtaaata aaataaaaag
    67021 gtggtttact catggcttta actccattag atatccaaaa taagacattc ccgacaaaaa
    67081 tgcggggata taatcaagac gaagtagatg atttcttaga tttagttgtt cgtgactatg
    67141 aggaattgac tcaaagaaat cgagaattag aaaaagcagt caaacattct gaagaaaaac
    67201 ttgagtactt caatgaatta aaagatgctt tgaaccaatc aatcatcgta gctcaagata
    67261 cggctgataa agtaaaaaca agtgcaagca aggaatccga agtgatcgtt acttctgcac
    67321 aaaacaaagc agatgagtta gttgcgaacg ctgaaaaacg tgcacatcaa ttaacaactg
    67381 atgcagaaga aaaagcaaga aaaatcttga cagatgcaac agaaaaagca agacagcttg
    67441 cgacagaaac tgaagatctt aagaaaaaaa cacgagtatt ccatcaacgt atcagcttga
    67501 tgcttgagtc tcaattagaa caagtgaaaa gtccggaatg ggatgaaatt ttacaaccat
    67561 tctccagcta cgtgactgac agccatgaag tgatcaaaga agttttagca aaacagcttg
    67621 acaatgaaaa tgatactgat gtaaactcag acacaggagt catagaagca cctatgacgg
    67681 catttgatac acaggctatt gatatctcga tcgtgcctgg tgaaaatgaa aacgagtaga
    67741 acagaaaagc actttgttat tcttaaagcg agccgatgag agtggaagtt cggtaagaca
    67801 ctgcatggtg aaagatcctc tacttgagac ctgcttttcc gaaattagag taagaagcag
    67861 cggctgacct cgttattggt cacagagagt ttgcactaga tagatgcaac taaatttggg
    67921 tggtaccacg gcatgttcgt cccttgcggc tgaacatgct tttttgtgtc cggaaaacaa
    67981 ataaaggagt gtatggttga atgaaaatga aagaaacctt gcaattagga aaaacaaaat
    68041 tccctatgcg tggtaatttg ccaaaccgag aaggcgagtg gcaaaaagaa tgggaagaaa
    68101 atcgaatcta ccaaaaaaga caagagttga acgaaggaaa acctagcttt gttcttcatg
    68161 atggacctcc atatgccaat ggtaatatcc atttaggaca ttcattgaat aaaatcagta
    68221 aagacattat cattcgttca aaatcaatgt ctggctatcg tgcaccatat gttccaggat
    68281 gggatacaca tggattgccg attgaacaag tattggctaa taaaggcgtg aaacgtaaag
    68341 aaatgtccat ggcagaatac ttggaaaaat gtcgtgaata tgcactttcc caagtagata
    68401 agcaacgagc agatttcaaa cgcctaggag ttgcaggaga ttgggaacat ccttatgtta
    68461 cattagatcc ttcttatgaa gctgcacaag tacgcgtatt tggtaaaatg gctgaaaaag
    68521 gctatattta caaaggattg aaaccaattt actggtctcc gtcaagtgag tcttctttag
    68581 cggaagctga aattgaatat aaagatgtaa aatctccatc tatttttgta gctttcaatg
    68641 tcaaagacgg caaagatatt cttgatgaag atacagcttt tgtcatttgg acgacaacac
    68701 catggacatt gccagcaaac caaggtattg ctgtaaatcc aaaatttacg tatgtattgg
    68761 tggaagccga tggccgtaag tttgtcgtag cgaaagattt gctagaaaca gtccaatcag
    68821 aaatcggttg ggaaaatgta acagttctaa aagaatttgc tggacaagaa atggaatata
    68881 tgactgcaag tcatccattc tatgaacgtg aatcacttgt gattttaggc gatcatgtta
    68941 ctttagacgc tggtactgga ttagtccata ctgctccagg acatggggaa gatgactata
    69001 tcgctggtag caaatataaa ctatctgtag tgtcacctgt agactcaaaa ggattgttta
    69061 cagaagaagc acctggattt gaaggtatct tttatgacaa agcaaatcct atgatcactg
    69121 atctattaaa agaaaaagga gctttgctga agctagattt ctttactcat agttatcctc
    69181 acgattggcg gacgaaaaag cctgtgatct atcgggcaac tccacaatgg tttgcatcta
    69241 tcgataaatt ccgtcagaat attttggatg aagtagaaaa agtagagtgg gttatcccat
    69301 ggggtaaaac acgtttgtat aatatgatcc gtgaccgcgg cgattgggta atttcccgtc
    69361 agcgtgcatg gggagtacca cttccgattt tctatgctga aaatggagaa gcaatcatca
    69421 caccagaaac aatcgaacat gtggcaaaat tatttgctga acatggatca attatctggt
    69481 ttgaaaaaga agcgaaagaa cttcttcctg aaggatttac ccacccaggt tctcctaatg
    69541 gcgaatttac aaaagaaaaa gatatcatgg atgtctggtt tgattctggt tcttctcatg
    69601 aagctgtttt acgtcagcga ccagaattat cattcccagc agatatgtat ttagaaggat
    69661 cagaccaata tcgtggctgg ttcaactcaa gtatcacgac tagtgtggct atcaatggtg
    69721 tagcaccata caaatcagtt atttctcaag gatttgcttt agatggtgaa ggccgtaaga
    69781 tgagtaaatc tttaggaaac atcattgcac cagaaaaagt gatcaaacaa tttggtgcag
    69841 atatccttcg cttatgggta tctagtgtag attatgaagc agatgttcgt gtttctatgg
    69901 atatcttaag tcaagtggca gaagtatatc gtaaaatcag aaatacgatg cgtttcttat
    69961 tagcgaatac agaagatttc gatcctaaaa aagatactgt ttcgtacaat gatttacgtt
    70021 ctgtagacaa atatatgacc gttcgattga accaagtaat caaagaaatc cgagaagaag
    70081 ggtacgacaa atataacttc atgcatattt accgtacagt tatgaacttc ttgacggttg
    70141 atctatcgtc attctacctg gattttgcta aagatgtagt ttatattgaa gcagaagatg
    70201 attatcaacg tcgttgcatg caaactgttt tctatcaaac agctgtagct ttgacaaaat
    70261 tattgactcc tatcattccg catacagcag aagaaatctg gtcattcctg aaagaagaag
    70321 aggagtacgt tcagttaagc gagtttcctg gttatgaaga gtttgcaaac caagaagaat
    70381 tgatggatac ctggactgct ttcatggact tccgtgataa tgtcttaaaa gcattggaag
    70441 aagcaagaaa cagcaaattg attggtaaat ctttggaagc aaaactaact gtttatccaa
    70501 aacaacaagt tcgtgaaatg ttaaaagctt tggatgcaga tattgcacaa ttattgatcg
    70561 tttcaccaga ctactttgaa gttatggcag cagatgaaca agtgccagat aacgctatgg
    70621 tatttgatga tgttgctatt acagtggaaa aagcagacgg ggaaacttgc gatcgctgtc
    70681 gccaagttcg taaagatgta ggagtagatg aaaaacttcc tcatctatgc ggtcgttgtg
    70741 caaaaatcgt tgaagacttc tatcctgaag ctgtagctga aggattcgaa gaaaagtaaa
    70801 agggaaaagg gggtgtgaca tgttctttgt cacaccccct tttgttatca atcgtttaat
    70861 tttccacgtt cacgatacca gttatctggt tgccagatcc attcgaatcc gtctttttca
    70921 agtagttcga aagctgcttt aggtcccatt gatccagctt tgtagttggg aaaatccggt
    70981 tgtgtttggt cccaagcttt tcgaatgaca tccacgatat gccaagattg agcgacttct
    71041 tcccaatgag agaagttcgt tccatcacca ttcaacgcat ctaatagtaa tttttcgtaa
    71101 gcttctgggc tattttcaac catttctgca ctatttctaa attccaattt gattggttcc
    71161 gtttcaaatc cttgccctac ttctttccca ttcaaggata aagaaaatcc ttcagttggc
    71221 tgaatataga tcgttagaac attaggtgga agtgtcgtat cgtcacatgg ttcatcgaca
    71281 gatgttttga atacattaac aggaacttgt ttgaatacga tgttgatccg agtccctttt
    71341 tcagtcaaac gtttgcctgt acgtacatag aaaggaacac ctgaccaacg gaagttatcg
    71401 atagtgaatt tgcctgcgac gaatgtttca gtctctgatt gagcatccac tttgtcttct
    71461 tcgcgatagc ccttgaattg tacaccatcc aattcacctt gagcatattg cccgcgaacg
    71521 aagttttcta acacttcttc ttcgttataa atgcggatag ctttcaatgc tttgatcttt
    71581 tctgtacgga tttctttttc ggaaaatgct actggcggtt ccattgcaag taatgctaca
    71641 acctgtaaaa tatgattttg caccatgtct ttaagtgcgc cactatgatc gtagtagccc
    71701 ccgcgatctt ctacaccgag gttttcgcca aaagtaattt ggatattgtc gatatagcga
    71761 ttgttccatt gagcctcaaa aatattattg gcaaaacgga tagctgagat gttttggatc
    71821 atttcttttc ctaaataatg atcaatcctg aaaatatctt gttctgggaa tacttctctg
    71881 atttgctggt tcaattcaaa agcagactgg taatcagaac cgaacggttt ttctatgatc
    71941 aaacgattga agccattttt tgtcataata ttttgtgatt tcaaatgaga aacgatcgtt
    72001 ccaaaaaatt gaggtgccat agccaaataa tagacacggt taccgtttaa ttgatagtgt
    72061 ccatctaatc ggtcagacaa ttcttttaat gtctgataat gcgttgaatc atttacatca
    72121 tgggattgat agtaaaaatg gcttgcaaag gtagttgctt cttcatctga aggattcaat
    72181 gcacggattg tttctttgac aacttcacgg tagtgctcat cgctccaggg acggcgagcg
    72241 gttccgatta cagcaaaatg ctcgcctaaa tttccttttc tgtaaaggcg gaacaaagac
    72301 gggtacaatt tacgctgggc taggtcacct gtaccgccga atatcgtaaa caatacattt
    72361 ttttcattag acatgtggat acactccttt atattttatc gacaaaaatc gtgctggccg
    72421 ccttaaagct gacggggatt ttctttctat cattttggat agtgatcggt ccttcataag
    72481 ctgcgatgtc aacgatctca tattcttcgt tgatattcag gtcaatagtg actaagtaat
    72541 ccaacaattc tttttcatca agcactcgtg cgatacgcac ttttgtaccg acaggatagg
    72601 actccaatgt ttgtcggcgt tcttcatgga caacttcctc ttttttagga atcgatcctc
    72661 catgaggaca atgttcagga tgattcaaat agttttctaa atgatctgca agatgttccg
    72721 aagtcacatg ttccaatact tcagcatcat catggacttc attccagcta tagtctaaat
    72781 gttcaactaa aaaaacttcc cataagcgat gtttgcgaat cagactgcta gctttttgca
    72841 gtccgcttgc agtcaattgt acaccttgat aaggggaatg ttctacaagc tcttctttca
    72901 caagtttaga aatcatttca ctgacagatg cagctgagac atctagacta gatacgattt
    72961 gtttattgtt gactttcgtt gtatctccac ctagttctaa aatgattttc aaataatctt
    73021 ctcgatttgg tgtcatattc gatcatcctt tcatggtaaa aaaccatcat tagtaagtgt
    73081 aacaaaaaaa acgttaattg actatttttc taaataaaaa acaaaggatt tttagacgtt
    73141 tttacaacaa aaaacgaaag atgatagaga agactcattc tttcgtttat aaaggatata
    73201 tatatacatt gggagggatc tatttttcag ataacaactt atatgaagtg tctgcagatt
    73261 cgtcataaag agtttgttcg ggataagaaa agataagata aaaaccatac tgatcacgta
    73321 cgactaaacg atattcatct atttctacca cagatgtgcc aagagactga ccgttgatca
    73381 aaaacgtgta atcagatgta atctgtaatt gttgtttttg ctggctatca ctaaagaacc
    73441 atgtaccttg atactttgag agtccttctg acttttccgc ttgttttttc tttttcaagt
    73501 aaataaaact tgagagaacg gtaatgagtg aaccaagaaa atagatcatt ttttttatca
    73561 ccaaatcact cctttatgag aaaataatcg catagacata ttctttcttc tttatttcat
    73621 tgtaccgaat aaaagctaaa atttctctaa gactttcgac tggttttttt actgtttttc
    73681 tacaaattat gataggatac atagtaggaa gaaaggtgac ataatttgaa gtatcagcaa
    73741 gtggaaatag ctttttttat tgaccgcccg aatcggttta tagcacattg tatgaaaaaa
    73801 gacggagaaa taattattgc gcatgtcaaa aatacaggta gaggaaaaga ggtcctgata
    73861 ccaggcgctg aagtggctat tgtatttgct cctggaccta aacggaaaac cgcctatgat
    73921 ctgattgctg taaaaaagaa gggtgactgg tttaatatcg atagtcaatt gcctaatcgg
    73981 ctggcaattg atggtatttt agacggcacg atccttttac caggattaac tggtccattt
    74041 acggtataca agcgtgaagt aacttatctt cactccaaat ttgatattta tttagagacg
    74101 gtggtcggac aaaaagcatt tgttgaagtc aaaggtatga cacttgaaaa taaagcgatt
    74161 ggtgcttttc ctgatgcgcc cacactcaga ggattgaaac atataggaga attaacgtat
    74221 gccgcacaag atggttatgc cgcttatgtc ttgtttattg ctcagttcga acatcttcat
    74281 ctggcaacga tccatgaaga aatgcaacca gcactagctg atatggttcg acatgcgcag
    74341 caaagcggag ttcaaatcct cgcctataat tgtcaagtat cggcagatga ggtaactgtc
    74401 aagcaggcca ttccatttga tgtagatatc ccttttgaag atcctataca atagaaagaa
    74461 ggtaaaataa tgatcaactt aggtattatc ggaacaaatt ggattactca tcaatttgtc
    74521 caagcagctt taagcacaaa aaaatatgaa ttgacagcgg tatattctag acatttggaa
    74581 aaagcacagg aatttggttc tcattatgaa ggggatatcg aattcgcaac agatttgaag
    74641 acttttttca atattactca tatggacacc atttatattg cttctccgaa tagtcttcac
    74701 tttagtcaag ctaagcaagc aattttggca ggaaaaaatg tgatagttga aaagccagct
    74761 ttttcgacgc caaaagaaat ggatgaaatc atccatttgg caaatgaaca caatgtgtat
    74821 ttttttgaag cagcaagaaa tatccatgaa tcaggtttta aaaagattgc cgattactta
    74881 ccattaaaag acgagataat cggtgcaaat ttcagctata tgaaatattc ttcgcgctat
    74941 gaccaagtat tagatggaga agaaccgaat atcttctcga ctcatttctc aggtggtgca
    75001 ttgatggatt taggtgttta ccttgtatat gcagctgttg cttggtttgg aatgccaaaa
    75061 gaagtccatt atttcccaag aaagatttct actggtgtag atggtcttgg atgggggatc
    75121 ttacgttatg agaattttga tgtatcgatc cagccaggta aaatcggtga ttcttattta
    75181 ccgtcagaga tttattttga ctcaggaacg ctagtgatga atggagtcaa tgcaattgaa
    75241 caagcacaat ttcatgatcg cgcgcataaa gaaatcattg atttggcggt aacagctgca
    75301 gaaaatccaa tggcagatga agcagaggat tttgccaatg tcatggctca tccgaaagat
    75361 ccagcttggg gattgttgta tgaagagtgg atcgagctag ctagaaatgt gaaccaagtg
    75421 atttatgatt taagaaaaaa tggcggcatc aaattcgatg cagataatga ggaagatttt
    75481 taatgaacga atttgtacaa gtatttcccg aaaattggca gaaaaaatat gaggataaag
    75541 ggtttatctc accttctctt attcaacgag cagtttttca gccactaagt aataaacaaa
    75601 gtatagtagc ggtttctcct acaggttcag gtaaaacact ggcttatctt tggcctctac
    75661 tactaaacgt ggaaccaggt gaagcaagtg cgttggtcat ttttgcttct tctcaagaac
    75721 tagctattca agtagcagat gttgcacgtg aatggggaaa agacaaagag ttgaaagtcc
    75781 aatctttggt gggcggagcc aacgtcaaac gtcagattga gggtttgaag gaaaaaccag
    75841 aaatcttgat tggtacacca ggaagaatat tggaattgat gaaagcaaaa aaaataaaag
    75901 cacatcaagt gaaaacaatg gtatttgatg aagcagacca gctatttgac gctgggaaca
    75961 gccaaatcat tgatcaaatt cttcatcaag ctccaacaga gtatcagcta gctttttttt
    76021 cagcgacagc agatcgttcc cttgaaagca ttgaaaaaat cactgggaaa acgtttgaga
    76081 caattgacgt gacagcagag gacgattctc gaaaaggact gagacattac tttttacgtg
    76141 taccgatacg aaagaaagat gactacttga gacgtctcac gcatatagaa gattttcatg
    76201 gtctgatttt ctttaatcaa ctgactgaat taggcgtaat ggaagaaaaa ttgctgtata
    76261 gaggtgtacc ggtaggaagt ctggcctctg atcagaacaa gctactccga aaagcagcgc
    76321 tagaacaatt caaaaagaaa atggtcagag cacttttgac cacagatgtg gcggctagag
    76381 gtttagatat tacaggtctt ccttatgtag tgaatgcaga agttccgtta agtgaagaag
    76441 cttatttgca tcgttcggga agaactggtc gaatgggtaa tgaaggaata gtaattacac
    76501 tcgtacaaga taataatcta agagatttaa aacggctttt acgtccatca aatataacac
    76561 ttgaagagat ttttttatac ggaggtaagc tgcaaactga gcagccaacc aaagaagatg
    76621 cagctcctgc agtaaataaa aagtatgcga aaactttttc tggaaaggag cagaacccag
    76681 aatataacaa agaaaaaata agaaaaaaag ttaaaaataa atcaaaaaaa gagagaaata
    76741 aaggagcaag gcgcaaataa gcgccttttt atgctatgat tatgaaaacg ggaacaaaat
    76801 tggttgccag aggaggggtt atgtgaaaga acgtcaagaa gagatcttgc agctattgac
    76861 agaaaaccaa caaggattga cagcaagtga tgtagctgag cgcttaacta tcgatcgaag
    76921 caatgcaagc cgatatttaa gtgaactata caaggcccat catattgtaa aaaccgctgg
    76981 ccgaccagtc gtttactctt tgccaacgga aaagtcaaaa tctgatgaag tgcatgtcga
    77041 ctcttctacg caagtgactt ttgaaacact cgttggagaa aatgattctt taaaagtcag
    77101 catacagcag gcaaaagctg cgattcttta tccaccgcgg gggttgcata cgattatttt
    77161 tggagaaact ggtacaggga agtcgatgtt tgctgaatgt atgtatcatt ttgcaatcga
    77221 ttcagagatg ctttctgcag atgcgccatt tgtttctttc aattgtgcgg attatgctca
    77281 aaatccgcaa ctgctctttg gacacatctt tgggatcaaa aaaggggctt acacaggagc
    77341 agctcaagat agtcccggtc taatagccaa agcagacggc ggtatattgt ttttagatga
    77401 gatccaccgt ttaccgccgg aagggcagga aatgctgttt actttcattg ataaaggtat
    77461 ttaccgtccg cttggagaaa gtgcacaagt acatgaagca tctgttcaga tcattggggc
    77521 gacgactgaa tcttcagaaa gtttcttatc tacgttcaat cgacggattc ctatggcaat
    77581 caccttgccg aatcttgctt cacgttcact ggatgagcgt tatgaaatca tctctctttt
    77641 tatcaaacaa gaagcgaatc gtttgaatca acgtatcgat atcgaaaaag aagcgattct
    77701 tgccttcatg ctttatgatg cggaaggaaa tatcggtcaa gtgaaacgag acttgaaact
    77761 agtctgtgca aaatcttttt tacattaccg tactcatcat gagaaaaagc taatcattcg
    77821 aaaagaagaa ctttcccttc aagtacaaaa aggattactg aaaatcaagg aaataccgga
    77881 acgtctcgac cgattcatgg acagcaaaag tcagtatttg acatttgaac caggatttgc
    77941 tgatgttgtc tggtcacaag atccagaacg aaacatgcag gtctataacg atattgagga
    78001 aaaagtcact tctctttccg aaaccggtgt ggagaacatt gatctagaga ccctcatttc
    78061 aaaagatgtg gatgcttatt tccagacata tgtcaaagaa ttgactaaaa gttcgattcc
    78121 caaagatctt ttgccagatg aaatctggcg gttaaccaat caattgtatg atgtggctga
    78181 aaaacggtta gaccgtattt ataatgaaaa agctcgtttt gcttttgctt tgcatctgca
    78241 aagtacattg gatcgtgtga gagaaggtca tatgatcgtc cacccagatt tgaataacgt
    78301 acggaaaaat tttaagaggg aattccaggt agcaatcgat ctttcaacga tcattgaaga
    78361 agaagaacaa gttgagattc cttttgatga aatcggcttt attagtatgt ttctttctat
    78421 caatctagga gaaagtgagc ctgttaaaga aaacaaagta gatgttctca tattgatgca
    78481 cggcagacag actgcttcaa gcatgttgga gatggcacaa gaactattag gaacaaaagt
    78541 agggaaagct tttaatatgc cactggttat ggaagttcaa acaatgtatg aagaagtgtt
    78601 acaatatgtc aaaaactatc aagagcattt gacgaatggc ctactcttgt taacagatat
    78661 gggatcatta aacaccttca gtgatttgat ttatgaagag actggcattc ggacgaaagc
    78721 tatcacgatg acaagtacca tgattgtgat agaagcgata cggatggcaa gtgttggaag
    78781 aagcttagaa gatatttatc aaaatatcca gctttccttt gaatcgatcg tacgtgatca
    78841 gttcaaagcg gaaaagtctg aaactttaca gcgtaaaaaa gccgtaatcg tgacatgctt
    78901 tactggtgaa ggagtagctg ccaaattata tcagcgtatt atgccagtga ttaatcaaaa
    78961 taaggtagaa ctgatccaga tgcagttttt agaacgtgaa tcattcaaaa aacacatcga
    79021 tgccttattg gaagaatatg agatcaaagc aattgcggga acagttgatg ttgattatca
    79081 aaatatccca tttttttcag cctatgacgt ttttgatgat gaaaaactaa atattttaaa
    79141 gcgtatagct gatgatgaga tcccgacagc taaaattgtc gattcacttg ctggatcatt
    79201 aagcgatgtc ttttctattg atgaactgat ccgttcattg caaaagacag ttcagcagat
    79261 ccagaccgat ttgtcgatta tcgtggaacc tggagtagat actggaatcg ttatccattt
    79321 agccttcatg ttagacggat tgatcaaaca agaaagccat cggcaatttg agaaactgtc
    79381 tattttctca aaaatttatc gtttggaaat ggatatcgtt cgggcaaact tgatggcact
    79441 agaacgaaaa tatcaggtaa aaattccaga agatgatatc gcatttctta cacaaatgtt
    79501 tcttgaaaat aaaataaaaa aatgaacaca ctaatcatac tgtttacaca ctaaacatct
    79561 gtgtgcgcac tataaaaaag agctaaaaag catgaaaacg ctatttcagc tctttttttt
    79621 attggcatgc ttcttgcgtt atataaaagt gtaaaaaaat aaaaggagtg ttaaaaatgg
    79681 caaaaaaaac aatcatgtta gtttgttcag cagggatgag tacgagttta ttggtcacaa
    79741 aaatgcaaaa agcagcagaa gaaaaaggga tggaagccga tatttttgcg gtctctgcct
    79801 cagatgcaga taataattta gaatctaaga atgtcgatgt attattatta ggtcctcaag
    79861 tacgtttcat gaaagctcaa tttgaacaaa aacttgcgcc taaaggtatt ccattagatg
    79921 tcatcaatat gcaagactat ggcatgatga acggagaaaa agttcttgca caagcagaaa
    79981 atctaatgaa ataaatcaag tatgcagttg ggggtataac aacgtggaag atcaacaaaa
    80041 tctagaagcc atcatggggt taatcatgtt tggcggtaat gcaaagagcg atgcaatgga
    80101 agcgattgcg gctgctaaaa tcggtgattt tgagttagcg gataggaaaa tcgctgatgc
    80161 agaagaatca cttatacaag cgcatcatgc acaaacagac atgttgacac aagaagcaca
    80221 aggaaatcat atgcaagtga cattattgac tgttcacagt caagatcact taatgacatc
    80281 tattgcgttt actgatttag caaaagaaat catcgaactt tatcgtcgcg tatacagcga
    80341 ataaatacaa cttctttttt gaacggaaga agaaaaattt ttgaacggat tttttatttt
    80401 gttataagga gaactctctt tatataaaaa acttatagga ggaagtaaaa aatgaacgga
    80461 ctatcaagtt ggatggagaa atacatcctg ccggttgccg caaaaatcgg agcgcaaaaa
    80521 catttagttg ctttgcgtga tgcttttatc ggcacactgc ccgcaacaat ggcgggatca
    80581 gtcgctgtaa tgatcaatgc aattattcgt gatttaccgc aacagtttaa cagtacctat
    80641 gctgacagtt tagcagcaga caaaggaatc tttgccgtat tgaatgtcat catcggaatc
    80701 aatggttttg tctggaacgg tacactagca atcgccggat tgatctttgc cttttcttgg
    80761 gggtataatc ttgcccgagc ttatggagta aatgatctag ctggggggat cgttggttta
    80821 gctaccttga ttcaaggtat ttcctttggt ccagcattat cagcagcatt agatgtgaat
    80881 gttccttcaa atgtcgcaga tgcaatcaat agttctgaga ttggtgcaac gattgcagat
    80941 ggcacactgt cagttggatt atggggttgg ctaaaactag atcatttgaa tggaaacgct
    81001 tattttacag ttatgatcat gggagctatt tcagtcatta ttttttgtaa attgatgctt
    81061 gccaacatta cgatcaaaat gcctgattca gtaccaccag cagtatctaa agcatttgca
    81121 gccattttac cagcgacgat cgctttatac gtcattgcta ttatcaatta tatcgtaggt
    81181 cgtgtagctg atgggcaact gatcattgat ttggtgcaaa aatatattgc agaaccattc
    81241 ctaggattat ctcaagggtt gggagcagta ttgatcgtca cagtatttgt tcaagtattc
    81301 tggttctttg gtattcatgg accaaatgta ttggcaccag tgctagaggg aatctgggga
    81361 caagcacaac taatcaatat tgatattttc caaaaaggtt ataacggaaa aactggtaca
    81421 ccagccgtgt tagatgcaat cgaagatgga aaagcttata tgtgggtccg cggatcgttc
    81481 gatgcttttg catggtttgg cggatcaggc ggaacgatcg tcttactgat agctatcttg
    81541 attttctcta aacgtgcaga ttatctaaca gtagctaaat tatccattgg gcctggtatc
    81601 ttcaatatca acgaaccagt gatgtttggt ctgccaatcg tattgaatgc aatcatgttt
    81661 atcccattct tgattgctcc agtagtagca acagcaattg gatggacagc tacttatctt
    81721 ggcctagtcg ctccggtatc gcagcaagtc acatgggtag taccgccgtt cttattgtca
    81781 ttcttagcaa caggagcgga ttggcgggca ccgatcgtaa cattagtatg tatggtcgtg
    81841 actttcttga tttgggcacc atttgtcatc tctgcaaaca aaatggaccc tgccgcaggc
    81901 gaacaacaat aagatgaacg tgaggagcgg gacgctttaa gtttcgttcc tcataatgaa
    81961 ttttcgagga ggaactgaaa tgattacaat tgccagtgca atgcaacaag atgaaatcaa
    82021 agaattgtta gaaggataca atgaagaagg aatgactttt acatttaaag aaaaacaagg
    82081 aatcaagctg ttctttgaaa caagtgcaga agatccggaa gttgcggcta aaaaagcaaa
    82141 ggaactaatc aaagcacagc cttggggaac agtcctttat tttcaatcaa cagctgttta
    82201 gaaaatggtt taaaaagata ggatggttga gatgataaaa aacggtttat ttttatgtag
    82261 tattggttct ttgatcgtgt tgtatagttt gaatccacat tcgcaaaggt atgatttgta
    82321 tagtatggtc acaggtcttt ttctaacagg attaggaatc tatttcttca tgaaggggaa
    82381 aaaaagagaa gaagcaaagg agaaaaataa ctaatggaaa tcacgaaaaa attaaatatt
    82441 ccagcatcgt ttttttacga taaagttatg gactctgtcc ttttcgatgt acgtaaagcg
    82501 accggcaaat cagtaactag aaaacagctg aaaaatttgg agtacgtcaa acaattctcg
    82561 caaaataacc gggctcgcat aaaaatcgaa gaagtcattg aaaaccagtc ttataagttt
    82621 cgtacatcga caacgaagaa tgaattcgtc gttcattatc aaatcaaacc attggacgat
    82681 tcgacctgtg aggttcatta cacagaaaaa atggagtctt atggcatgct tcaaaagttg
    82741 aatgatatgc ttttaggaac aatcttgatg tattttaaac ggagacgttt caaaaaaatg
    82801 ctagatatga tcgaagcaac gtattaaatc aatgaaacat ggtactattt agttattgaa
    82861 aaatgaagta agctagaagg aatttttcat aggaagatag atctttcctg cgaatcctca
    82921 ctagcttttt tgtaggaagg ggcagcaaaa tgacaaacaa agtaagagag ctaggtgtat
    82981 cttcgattga ggaaatgttt gatttagcca cttatgcatt taatggggca gatacacctg
    83041 aaaggcgaga acgttttcgt acacttgcgg agaattcttg gaattacgga ttttttgatg
    83101 aggaggatcg gctaacaagt caagtcatgg cgaccccttt tccagtgaat ttttacggac
    83161 aagagtacct aatggccggc attggctatg ttgcttctta tccagaagca aggggacaag
    83221 gcgggatcaa tcagatcatg gaaaagatcc ttgaagattg ccgtgatcgt aaagtaagct
    83281 tatcttatct ggcgccattc tcctatccct tctatcggcg ctatggctat gaacaatcat
    83341 ttgataaaat cagttatcaa ctaaacagtc gtgatgtgcc gtttatcaaa aaaaaatctg
    83401 gaaaaataaa gcgtatggat tttgaagatg caaaagctgc aatcagaaga atttatgaac
    83461 agatgccgga aaaccaacga gctggtctga agcgcgaaga ctggtggtat acgtataaat
    83521 tcaagcaaaa aggcaattac cagtttgctt tgcattttga tgataatgag gaagcaacgg
    83581 gatatatcgt ttatcagctt gcaacgtctt cctttattat tgcggagtgg ggatttttgg
    83641 atcatgatgc tttttgcagc cttgttcgct tcgtaagttc tcacaatggt gcttttgaaa
    83701 catttgaata ttcatgcggc tacggcggta atgatcaaaa ttatctatta gaaaatccat
    83761 ttgcttctgt aatgatcaca ccttatatga tggcgaggat cgtagatatt tctttgtttt
    83821 tggaaaatta tccatttaaa aagagacaag ctgcatttgc cctacaaata gaagaagatc
    83881 attatgcgaa atggaatgaa ggaatcgtgg aagtaacaat cgaaaatgga aaaaatcatc
    83941 tctcaaaagt agaacaaaca aaactcccaa tcgttcgagg aagtatccaa catatcacgc
    84001 agctattatt agggtatcgt aaagtggaag agttggtatt ccaagaaaga atacaggtga
    84061 caaaagaata tgaagaaacg ttaagctcaa tcttgccaca gcaacagcca attcttaatg
    84121 actatttttg ataaaaaacg ggtgatgaca atatttttgt caccaccctt ttgtttttca
    84181 tttggcattt gaaggaaaat gttaaagtaa atcctctttt ttcgtaaatt tgaatttttt
    84241 gttttctttt gttttagggt tttctttttt gtggtcttgg attgcttctt tcgtaatctt
    84301 ttcccgattt tttttagatg aatctaagcg ttctgcgtat actttttctt ttggcttatc
    84361 attcattgga aaggacctcc ctacattatt tcattctacc tctagtttat catctgttaa
    84421 ggactagtgg aaacgttttg cctaacttat aagctgattt ttttagcaga taagcttgca
    84481 agtaaaaaac aaacaagcta caattgattt attgaaattg ttcctgtagc tcagtaggat
    84541 agagcaatcg cctcctaagc gataggtcgc tggttcaatt ccagtcagga acgtataaat
    84601 aacccctgta tcctttgcgg tacaggggtt tgttttgtat ttgtgccaaa aatgtgccaa
    84661 actagttatc atttttatat aaactatcga acacagtatt gattttttct ttatcctgat
    84721 cttcaagctc tcgaacaata tgaagataag tagacattgt agtttctaaa ctgctatgtc
    84781 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgtg
    84841 tatgccttag tccatgcaaa gtaaattgct tttcaatccc taattctttt aattttttgc
    84901 gcaaatattt gctgacggcg tttgatgaaa ctaatccatt ttttaagttg aaaaaaacaa
    84961 aattatgagg gtttttgatt tcaaaattct cgaataattc tttttgattg attttaaact
    85021 tttttaagag atcaatcgta tgtttatcaa tggaaatttt cctatttgaa gtttcgtttt
    85081 ttgtttttcc ccattcattt aatttataat tccatgtttt attaatagtg atgatttgat
    85141 cttcgaaatc gatatcttcc catgttaatc ctagaagttc agcaaatcgc attccagtgg
    85201 caccagcaac taacacgagc ataggagaag agtactgcgc agaaaggttt tcttctacga
    85261 gtttcattaa agatttaaat tgatcataat ctaaatattt atcttctttt ggcttcaatg
    85321 aatcttttcc tttgattact gcttttctag ttggatcaaa cggaattaat ccttcttcta
    85381 cggcatcttt caacgacgct ctaatatggt tattaaactt aataacggta gattttacat
    85441 ggtctttagc atacttattt aaaaagcgtt gatatcccac tctatctaaa tcagaaatca
    85501 ataccgctgg catatatttc tttatgttca ttaacgtatc ttcgtatttt ctataagtga
    85561 taggagatac tgttccttct ttataaagtt gcatccaatc ctcaaagtaa tctgataata
    85621 atagattttt gcgatcccct ttaaggcctt tgttcaagtt atattctaat tcgttggcgg
    85681 catcttttgc ttctcctttt gtcttaaatc ctgattttct tatcttcgtg tattttccgt
    85741 catctttttt gtatgaaatt tcgtattgcc aactatttcc acgtttgact aatcttgcca
    85801 taattgaatc agctctcttt ctctgataca ataggaacta taaagaagcc cattgtttgg
    85861 gttttattat tttttgcaca tcctttttgc ttgccggcgc aaggatgtgc tatttgtcgt
    85921 tttaatggta taattggatg tgtattagat catccaatca gaggtgttta ttatgattaa
    85981 agtgattttt aaaagaataa ctgaacttcc agagtttaga ttatttgttg attattggta
    86041 tgtctggttt tttattgttg cattgctgat aggtctagat gtcatattag aaagaaaact
    86101 taagtaagag ttgagttaat aaattgaatt cctaaatgag atattttgta gcccatattt
    86161 tgtttgccga catcacctaa tactatggag ctaatcgttt tttctaacag accaaaatca
    86221 gataatctat ttaagctacc atctattagc tcttgtgaat agccagacat gctgatattt
    86281 ccagtgaaat tttcgtttcc acgactcatt aagcatgcta acaattgaat ctcaatcgat
    86341 gttaattgat tcagtacaag aagataatac tcctcgttat cccatgaagc gttgttaatt
    86401 cgaagtaaac aattcttata aagattttta tacatatcta ttttattttg tgcctttgat
    86461 ttttctacct cgtcatttat cccttcgaat attccaatga gttggtcctt gttctctaag
    86521 ttgaaaacat cttctggaat ttgatcgcga atgttttcta atgattcgtt taaatttttg
    86581 tagaatttct cgatgcgttt gaaacgcttc tcgtttttag acccaaaata aagtgactgc
    86641 acaacgccac caatataagg aattgctcca ataccgcctt caactattaa ttcaagatat
    86701 tcttttttgg ttagtttttc attgctatta tccatagtta cctcctattt tgtcatttcc
    86761 tttacaagca ttgtgactcc tgatggcact cttttgtata ggttttatta ttgtgcacaa
    86821 tctaactctt agtggggcgt ggttcattta ttttttactt actttactat taatcatatc
    86881 ccattgagca taggattttt ctatgttcga tcctcttgaa ctaatttctc ctgtaaaagt
    86941 tactttatcg ccctttgtaa aggtatcagc attgtttaag tccttcgcaa aaattacata
    87001 tgaaacttta ggtgttgcag aaatatcatt ccaactttta tcatcgtact ctttgtctgc
    87061 tataatggct attctagtag tcattgattc gataacagtt ccttcaaaag tatatgtttt
    87121 accactaata tatttagtaa aagctgtatt ttgctcagta gctggagtaa tagaataata
    87181 actatctaag aaagcacccc agtcatgatt atttgtctct aaaaattctt caaattgagt
    87241 taggctttta ttgctgctgt tagatgtagt agtttgagta tcagatgatg agctttcctg
    87301 tttgttattg tcatttgcct cgtttgatga ggaaagcgag aaacctacgg tagtaaaaat
    87361 aatcccgata ataaggagaa tcataggtgt tttcttattt gtttttttga taaatcttac
    87421 aattaacatg attaaaccta taataaataa aacaatacca gtaaaaccaa atataccgtc
    87481 cattaataga tcctctttct cttttgatat tgaatattat aaaccaagca attctttttt
    87541 cttaaaatcg aattcttctt gattaataat cccttcatct aacaactctt tatacttttt
    87601 aatttcatct gcacttgaaa tttgtgtttc tttggaatta ttatctaata tagattgttg
    87661 cttctctata tattttttta aattttccat ttcattccaa tatttttttg taaacataac
    87721 ggtgttttca tctttcgtgg ccgctaatat acctccgcga ctttcgtttc cgcctagcaa
    87781 ggtaaattgt atataaccat tactcatacc tggttttttt aattgtacgg ctgatatatt
    87841 tttaaagggg atagtttttt ctcctttaat accttgatta actaaattta taaatccttt
    87901 tctaataatt gaaatagaat catcagttac tttaatcaag gtttttcctg gagattttat
    87961 taaaatctct ttttccattt tttcttcctc atttctgtga tatgattttt tttgtagaag
    88021 ctcatatatg aggaaagagc ccgtgttgca gcacgggctt ttttaatgtt ttgctgaaat
    88081 cggttttttt aataattctt gatgtattca tacatattgc cttgcgtaag aatatttttt
    88141 cttaaaatag cattggcaga cagcataaca agtgtatctg agcttattct gaatagaata
    88201 atactccata aatttttcga gattaaactg agattcatca gtcagttcgt tctcaatata
    88261 gatatttaat aaaattagaa tagctatttt atctgcttct gtttcaaatt ttgagtgaaa
    88321 agttgtagaa gtatcgtaca aaactgaaaa ttcaaaatga gaggcgctga aatgtgcaag
    88381 ttcatgagat aaatgaaagg cttctgcagt ttcaccgtat agattttcat tcaaaaaaat
    88441 gattctgggt tttggatagt agaaaccagg ctctttcatc tccatataaa ctactttcaa
    88501 attgtattcg ctcaacattt ctttcaattt caaatacata caaaccatca ctccaactat
    88561 tcattttcct ctaaagcttt agcaattgca atcgctttac gcattgtctc cttagatatt
    88621 tcttttccat caaaagaaaa aacagtatcg tcttctgata aatccacatg tttaggggtt
    88681 tctctttctt ctctacctag aaggtagtct acagagacat cgaaatagtc agcaatccga
    88741 cttaattcat ctgaattagg agtattattt ttccactttg ctagataacc gttagaatat
    88801 ccaaaattaa tttctaactg ccgtatagat atctttttcc tcttcgccaa gtcttttatt
    88861 atttcatagg tattcatcga taaaacaacc tttctaaatg tttacaaaaa aagtttagaa
    88921 aaatacgcag aaataatttg actaattctg agtaatgagc tatactatat cttgtaaaca
    88981 gatttaacaa ctaaaaagac aacaaaaaac actattgata tgtaaatgca gaccgccaag
    89041 aaagctttaa aatcaatgtt tttatgtctt atttaattat gctcttagta tagagtatta
    89101 ctcagatact gtcaattgaa tttagaaaaa agttgttaaa tttgtttacg aatataaaag
    89161 aaaggaatga gaaatatgaa cacaccgcaa atttttaatt tcgaacaaaa cgaagttcgg
    89221 acaattttag taaatgatga accatatttt gtaggcaaag acgttgcgag tgttttgggt
    89281 tattcaaaca ctaaagatgc tttgtcgcgg catgtagatt tggaagataa gatggggtcg
    89341 cgaatcacga cctcaggtca atcaagagag atgacaatca tcaacgaatc tggtttgtac
    89401 agtttaatct taaaatcaaa acttccctct gccaaaaaat ttaaacgttg ggtaacaagt
    89461 gaagtgttgc cagcaattag aaaacatgga ggttatctaa ctccagaaaa agtagaagaa
    89521 gctttgctta atccagatac aatcattcaa ttagcaactc aactaaaaga agaaagaact
    89581 ggaagattaa tcgcagaaca aaagattgca gagtacgaac ctaaaatctc ctatttagat
    89641 agcatattat cttctacaga ttcagtaaca attagtcaga ttgcagcaga ttatgggatg
    89701 tctccacaac agatgaataa attacttcat aaactaggta ttcagaaaaa agtcggtaac
    89761 caatggttat tgtgcaaaaa acacatgaac caaggataca caaaatctca tacaactgag
    89821 atcccgaaag ccgatggtgg cactaaaatt gtaatgaata ccaaatggac acagaaaggg
    89881 cgtctattta tctacgaatt actaaaaaaa gaaggatatt accctcaaat ggatttagag
    89941 gaaattggtt agaaaggagt tttagtatga ctgacattgc agaaatcact caacgagata
    90001 gagaaaaaat caaagaatat gtcgaaagtt caaagttctt aacttacacc atgcttgctg
    90061 aaagatttgg aattagcaaa agctacttat ctttaatttt aaacggtaaa aagacttctg
    90121 cagaagcaaa cagaattata gattcgatta tcactatgta cgaattgtaa agggaggaac
    90181 agctaatgca atatctagaa gcaaaaatcc caattccaga aggctatgta attatctccc
    90241 aagtggatta tgaggagtta aaaaaagctg atgatactgg tagatggatg acgttgccag
    90301 aagtgctaga acggattaac agaaaatatg attggtttac ttctagagtt ttaaagaacc
    90361 caagatatag aaatattatc gatatcgaga aaaacaaaaa tggatttgtc tattatccag
    90421 ttgaaggaag agacacatat ctatttttaa gaagtaaaac acttgaattt ttagaaacaa
    90481 atttttcgga aatcttaagg aggcaagcgg atgggaaaat ttaatagagc gctagtgttc
    90541 agcgcaccgc taatcatcta cgctttagga ctttggggaa gcaggcaagc gttgatagga
    90601 acgattgttt acatggtttg gatttttatg gggcttgatg aagctgaagc tgagtacaga
    90661 gcgaaaaagc caaccgaggg ggctgactaa aaatgaatag aaaagagaaa ctagaatgta
    90721 tattactatt actcagttta attctttcac taatttctct attgggaagt ttgtattttt
    90781 gatatcaaca attttgtgga tttgggttct atttagagta aatatcattt ttgtttggtg
    90841 attaaggagt ttattaaaat taattttccc tacttcaaat gcaaggtaac aagatatagc
    90901 acttttagga ggaatattta gcggtacagc ttcagagtat attctattat catcgactga
    90961 tcctccttta gcaattaatt tcttaaatgg agtagctgac catctgcggt ttaagttagc
    91021 ggatgaaagt tctaaattaa ctaacatagc tggttctgtt gaatagttag aaatgattac
    91081 ttttgtataa atcatatctg cagcgaagta agaagcatta agttcaactt ctagttgagg
    91141 gcgcattcct tttattttta tccctgttaa aaacgtactc aatataaaac ttattattga
    91201 catccattga aaaattgtta agtgaaaaaa attcaaaata aacaccacca gtttttaact
    91261 aaattatacc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg aaaaataaag
    91321 aagctgtgac tacgagctta caagtggcag aaagcttcga gaaaaaacat caacatgttt
    91381 taagagatat tgatgcacta aaaaaagatg tgtccaattt tggacagatg tttgtagaag
    91441 gtaatgaacc agattcatat ggcagaaatc gacgagtttt cttcattagt agagatggat
    91501 ttttcttgct ggctatgggg tttacaggaa agaaagctat ctacttcaaa caaaaataca
    91561 ttgaagcatt caacgaaatg gaagatgtta ttcgcaagaa tactgttcct caaacaattg
    91621 aagatatgat gatctatcaa ttagaagaaa tgaaagatgt taaaaaagat gtttccatgc
    91681 ttaaagatac tatgcgaatt agcggacaac aagagtttga aattaagcaa aaaggaaata
    91741 tgaaagttat gggagttcta gggggaaaag aaagccgagc ttatgaagaa atcagcaaaa
    91801 aagtattctc aaaattttgg tctgaattta aacgtacctt ttcaatccca agatatggcg
    91861 agttacctcg taagagattc gatgatgctg tttcatttat tgaaatgtgg ttgccagaaa
    91921 ctgcgatccg tatggaaatt gatcaactga acagacaaca gagacttttt ggtgatgaca
    91981 atgaatagag ctgaagcgct aagaataggg acggcaattg ctaattgctg gtggaaatac
    92041 tataaaccaa tcatcctaag ccaacaacat attgacaagc aaaaagcatg gcaacaaata
    92101 aaaaagtgac tccgccggca agcaaagagt cacaaagaaa acacatcata aggagatttt
    92161 agcatatgga aaaagaactt tccactctag atcaatattt gattgatcct gattggggca
    92221 agccgaaaat tgaggaaaca agtggtcgaa aaatcagacg aaatcttttg acgaatgaag
    92281 aactagcttg ggatcaagat gatttaggca accatgtaac tatttgggat catgtttatc
    92341 ttatccatct atcgaagcat tcgaataaac ctgaatatat ttacgtcatc gaagatggct
    92401 tgattgatgc gctagaagag tacgacagag ataacttgat tgatatctct tattacggac
    92461 caggtaagaa atacattgct gaaatggagg cagaatttga tgagtgaaat caaagggaca
    92521 acgaactttg aaaaactttt tagtcgtaag ttaaataaaa ttctcaagaa aaaaggaaat
    92581 tttgattatt tatcttgggc tcacgcgtgg gagattatga aaaagaatga tccacaggca
    92641 acggtaacta ttaatgagta caaacactac agggttgttt ctggaactca tcaagacttt
    92701 cttgttgagg aatataaacc ttttcttatg gatgaaactg ggacttatgt atctgtctca
    92761 gtaacggtta aaggacacac ggaaaccgaa ttatttcctg ttttagatta tcgaaaccaa
    92821 ccagttgtta aaccaaatgc aatgcaaatc aataactcat tgaagcgatg ctttgtgaaa
    92881 gcattggctc tacacggact gggattatat gtatttcaag gggaagatat tccaacacca
    92941 cctagaatcg atacaaagaa attaaacatg ctagagacga ttctagaagc tttcaatgag
    93001 cagatgggta aagatatgac caaaacctta atcgaatatg ttaatgagca gacagataaa
    93061 ttagggctct tagctgataa cgttgaaact attgaacagt taagctatga gcaatgtgcc
    93121 ttgatggagc gagcaatagc agctaagaga aaagaattag ataagaagtg atatgagtgt
    93181 ttaaaccatt aatcgattca tattcagcgg ttctgaaaaa gttcaaagga aaagacataa
    93241 gcgcaaccat caatgaggaa gtgaacattg atcgactaaa gacgatgtat gacggctacg
    93301 atggtgatcg agtcattgaa attcgtttta ttgatcctag acgtttcacc gtacagcaac
    93361 gaaacttcat ctatgcgctg ataggcgata tttttatcga tacaggcatg ccaacggact
    93421 tctggaagga attcttctac tttcgttttg aaggtgtcac agggcgcaaa ataagcctga
    93481 aagacgaatc gaatacaact gtgagtgatg ccaatgtctt agcaaatatc atcttagatt
    93541 tcatctttga acatcatatt cctttcaaag aaggctatga gattttacct gcgaatcaag
    93601 aatattactt ctacaaatgc atcacaaaaa gagtctgctg catctgtggc aaaacaggag
    93661 ctgacatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa gttgatcatg
    93721 cagagtacac atttgcagca ctctgcagaa tccatcacac agagaagcac aaaataggtg
    93781 tgattaattt caaaaataaa tatcaaatca aagggatcaa attaaaccag aaaacaatca
    93841 aaaagttaaa tattggaggg taaaaatgac agaacatcga agttattatg cgattatacc
    93901 agccaacgta aggtacgaca aaagacttaa accaaatact aagttgttat acggagagat
    93961 aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc aatgagtact ttgcagattt
    94021 atatggtgtg aataaagaga ccatatcgcg atgggtaagt gatttgatta agtttggata
    94081 cttgaatcgg gaaatcattt acaaagaggg taccaatcaa ataatcaata ggtacctacg
    94141 aattaatcaa taccctattg acgaaaaacg caatacccct attgacgaaa aagtcaaaga
    94201 taataataca tctattaata atacatttaa taatacaaaa gaatatataa gagagttacc
    94261 gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt ataaaaatgt
    94321 tcttttgtca gatgagcaga tggagaaact caaaacagaa ttccctaatg attaccaaga
    94381 gcgaatcgaa cgactgtcag agtattgtga atcatctggt aagacttata aaaactattt
    94441 ggcaactatt cgaagttggg caaggaaaga aaaaagtgaa cctaagaacg caagcagtgg
    94501 atacaagcgc acaggaagac gagagaagct tcctgaatgg gcaatcgacc aagaagccta
    94561 tctcaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat tctaagaggt
    94621 ggaaaaatga agatcgatta tctagaacta attaatgaaa tagcgaatta taaaaagggc
    94681 gaggaattag acgtcctgag agacgtatat gatcaactcg aagaagctgg aattgaagga
    94741 attaagaatg atcgttcgag ttggagtaag ctcagatact atttcgcact ttatatcgat
    94801 acaacacaat taagaaattt agcatatacc aaattactat ttgttgattg tgttaaaggc
    94861 ttgcaaaaac atcttaatga acttgagcag gtgtaatcag atggacctaa agacatttac
    94921 agcacagatt gaactaatgc atcaagaagc tttaagacaa agtgtatcgt acgaagacaa
    94981 gtggctcaac acgttccatg gcggacgtga gagcgcactt gatcaagtac tcaaattact
    95041 gaaaggagaa tgtcgggatg gataagaaag cggcaatgca gcgaattatc gaattgactt
    95101 attcagaaga ttggcaaaat gacaaagaag ctgcttcaga agtgatgagg cttggaagag
    95161 cgatgtgggc agacaagagc aacaagccaa gaccacgaaa aatcgcaatt tggcacggtg
    95221 acaaacttct agtgataggg acagctgaac agttagcaag tctcacaggc ttgcacgaga
    95281 aaatcgtgag gaaaagagca aggtgtggct acacagacgc taagaagaga acgtttagat
    95341 acgtggagga atcatcatga caacagaaga agtgattcaa atgcgtattc gaagccttca
    95401 gcgtgagatt gacgatctgg aacgaacaaa ggcagtgatg gtcaatgaaa cggcgagaaa
    95461 ggcaatcgat ttgcacatag agaatttaag aagggaaatc catcgattgg aggaatgagc
    95521 gtggataaga aagcagcaat gaaaagaatt gctgaattaa ccaagtcaga atcttggcaa
    95581 gaagacaaag aaatagttgc agaagtccaa aagctcggta aatcaatgtg gactgaaaag
    95641 cccaaacgga gaacgccgag aaaaattgca atctggcatg atgaccgaat tttagtaaca
    95701 ggtactgctg aacagttatc tgaaattact ggattaagca aaaacattat ctgggataga
    95761 gctaggagct tatggattga ttcaaaagga cgacagttta ggtatgtgga ggagagataa
    95821 tggatctcat tacacaatac agtgacatca tcctcaagaa aatcatgatg aagattcaga
    95881 aagataaaaa atcaaaagaa cgagctgaat tagttaagtt agaaatggct gaaacaggag
    95941 caggagtgcg aagtagcagg cattggaaag cagcagcaaa cattgaattt tattacaacg
    96001 aaattcaaaa agggttcgat cagatgcgtg agctggatcg gcaaacaaat tggagcaaga
    96061 aacttcatca agatcgtttc aaatttgtag agaagtataa agaaatatta gaagagtatt
    96121 tgaggaggac agcaaatgat aaaaaaactc gttcaattca gcatggattt atatgatatc
    96181 gaatcaggag caacactatc tgtggaatcg gaccatctaa tcataaattt tggtggaaag
    96241 cgccagatta ttttgtgggt agttgatgat gtactgtttc cagaaattgt tcatgatttc
    96301 gaagaatcaa aagcggttga gtttgaaata gtgaaaaaag taatggaatt gattgaaaaa
    96361 tacgaggagg acagcgaatg ataccgaagt ttagagcgtg ggataaacga aagaacgtaa
    96421 tgagagatgt agccgtcttg cattttacta aaaacggcaa aacaaacttt attgaatatt
    96481 ggataaatcc taccgaattg aaatcatatc acgtgcgaaa catcgacctc atgcaatcca
    96541 caggaatgaa agataagaat ggtgtggata tatttgaagg ggatatagta ttagtcagcg
    96601 tgcgaaatgg cttcgattac ttagataata aagtctgtat tgtcaaaaat tcaatagatt
    96661 attccggatt agtttgtgcc actgttgatg aagacttaga gtatcaaatt tttaacacag
    96721 agctgtttga agaatacacg tacgaagtca ccggaaatat atacgagaat agcgagttat
    96781 tggaggaaca gcgatgaata aacaggaagc aatcaaaaag ttagaaagta ttaaagcgat
    96841 aggaaatgat gcaatagctg cttgctataa cgagagtata aattcaggta ttacgttaat
    96901 gaaaaaaata gacgaaccgc agaaaccagt tattccacaa ctcgtggccg gttggcttga
    96961 gaaatctacg gaccctttta caaaagctga aaaaatagcg tatttaatca aatctaaaga
    97021 tggtgattca tattatttct gtgattggtt tgtacgagat ggcatagtga cgcaagagca
    97081 aggagaagaa ttactcgctt gggcaacaag acaatcatat gaaacactat tgagcctata
    97141 caacggctac gaggttgaga aagagccgtt atgggcaata aagaatgccg atggaaacta
    97201 tcttactaaa tgtgctttat ggggaaaaga tggagtaaat tatagttttg aatgcaatcc
    97261 atctcatcga ttgcttttca ctgataaagc aacagcggat gctgcagcat tgttggtgaa
    97321 tggaacagtg gaagaggtgg tagaaagatg aaactaaaag acggatttta cgctagcagt
    97381 cacggtatcg gcggtttaat gctagatatg ccgacaaaga accctaaaac acgcaagaaa
    97441 tcaaaattca aagtcggtga catggtccgc tgtgaagcag aagagttcgt ttatccgttc
    97501 agaggatacg ttaaaaagat actgtcaaac tcagcaatca ttcgtattga aaatacaatg
    97561 aagtgtgatc gttcgactgc aaaacacaag cattatttag ctgtagcgag attagttgac
    97621 atggaagtaa tcaagagcaa ataaaaaaag ccggatcgct ccgactgatt caataaatcc
    97681 aacacattta ttatatcaca taaaggagcg gtttgacttg atgcaattgt tacgagaggt
    97741 agatttcaaa cagacaagat gtaatgcgag agatgtgctg aagaactttc ggcgtttgga
    97801 gcggatggca ggtcgctctt tgatagatat taagtcgccg attataacgg atatgccgaa
    97861 ggcaccgaag catggcaata aggcagaaga cgcgattatt cagatgatgg atatagaaac
    97921 ggagagagat gcgattctag cggctttgat ggctcttagt ctgattagtc gtcagatact
    97981 ctactacagc ttctgtgtgc cagatagctt ctcaaactac agaattagcc gtgaagtggg
    98041 ttattcagaa agaagtatac aacggatgaa gtcggaagct ctaatagagt ttgcagaagc
    98101 atataaacac ggaaaaataa ttgcttataa ataatttggc ggttttttgg cggaatgatg
    98161 gcggttttta gacatttacc agtgttatta tggtagtgtc gaaagattag gaaacaggat
    98221 cgacaaaata aacgaaggga ggcaatctcc ctcatcgttt taaacttctt cttagataga
    98281 cagcaactaa ttatgaagaa aaggatgtga attcaactcc tcctaaattc ttcatcggtt
    98341 gctgtttatt ttgattagac agcagcactc acaaaactaa acatgttctt gtcgtctttc
    98401 aactgctgct gtctattaat ttatgtattg gagggaagaa aaatgaaatt atcagtagaa
    98461 ggcactccag aagaaataca agaattgctc caagctattg gtagtagcaa ggagcaaaag
    98521 gtaagtgttt ctaattcaga tattgacggt atttgcaatt gtttaaacag agaaaaaata
    98581 tctaatcctt actaagagaa aatatgttcg ttaatgaatt tccaattttc ctctgtagct
    98641 ctaccttgat aatttttttg cagttcacag atgaataagg aatcgttatt atcaagtact
    98701 gtatttaatt tttcacacat ttgatttgga ttcaaattag ttttaactaa ccaaaaagat
    98761 ttttgtagct ttatatatgc cccaaattct tttattattt caaatacttt atcatacttt
    98821 tgccctgggt tattaagatc ataactaatc atgtagggtt tatacatgtt gtcttgcaca
    98881 tccttgtttt atatttcagc ggaccactcg ctgataaata aaattatacg cttagtattt
    98941 attttcacaa tattaattta tcgtaataac ttttgagtaa atagaacaaa aaacctgcac
    99001 tagtttccgc tagtacaggc agtgactata tcggttgata atctagcata tattaaaaat
    99061 aattgcaaga aaagaaattt atagtggttt ataccaaatt atcaaagcaa tgttattttg
    99121 ttgctgtcta ttgtttttta attattcaca cgataactaa aggtgggtga agagaaatga
    99181 ttccattaat aatttcaatt tttgcgctct gtcttaatgt ctatatgatt ggatttaaaa
    99241 atgggcaaaa taaaaaatag tagcagccaa gaataatttt atagtgtcac tgtggcggaa
    99301 agggtagacg ctaagcatgt gtgctaggtc aatgcttcgg caaccatgca aggttcgatt
    99361 ccttgccagc gacattaaat gcctatgacg gttacgacta ccgaaaaaag atcgttaaga
    99421 agctatacgg tgctacgtac ggcaatgtag taagtgtgct atctgtacac caccaagctt
    99481 cggtcactgt ggcggaagta gaagacgcag cggtaaatgg cgagtagcct cgtgagagcc
    99541 tggtaagttc tcgtgagtgg tgcaatccca ctccagcgac tttaagcaac cgaggcatcg
    99601 gcggtttaaa aatatagggg tgcgcaattt cgtacgcgtt ttgtgcatcg tgcaagttac
    99661 tattacatat tagatcactc tttgagtggt ctttttattt ttgcacaaag gaggaaacaa
    99721 caatgtatag accacaatac ttagaacaga agtatgaagt aatcactgtt cataaaggta
    99781 atggcgaaaa agtatatgag tatagaagac caataaagag cgatacatat aaacgaaagg
    99841 aaaacaatga agttattcca ttgtatggca aaagaatagc taagcattaa ataagattgc
    99901 gaaaggagac ggaacatgac cgaggaattc tatagatggc tattacagtt gacaagagaa
    99961 gatcgtttgg ttaagttcta tcagtctcct aaatggcgca ggcttagaga gaaagcgatg
   100021 aaacgagatc actatgaatg ccaagagtgt agaagactag gtaagtatca tagagtagag
   100081 aacgttcatc atataaagga agtcaaggat agacctgact tagctttaga tttagataat
   100141 cttatttgtt tatgtgttga acatcataat gaagttcatg gcagatatct tacagcatta
   100201 gataaacaag agaagaagat agaaagcttt gctaacttcg atgcaagtga aaggtggtaa
   100261 gtgcatgatc atcaatgata atggcagaga gtatgataca gaaaagattg aagagtattc
   100321 atcttatact cagggattaa ttaaacgttt gatatacgtt cgctatgtag gtattaggga
   100381 tctgttatca gataactgtt gtagtaaata caaagtgaat caagtaagag aagcgttgaa
   100441 taaagataat aacgtcgaaa gaataaaaaa tgtttttgga tatagtattg aagagattaa
   100501 ttattacatt gacttcgctg aagctttcat tccgatggtg agataacccc cccttaaaat
   100561 aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct gtcaggaaaa gagatttttt
   100621 cgaactttat catgaaagga gggctaaaat gtttaaaaac gaattgtctc aaaatcgcta
   100681 cagagaaaaa ttacgccgct ctttaataag ccaattggaa agtcagaaaa caaatattga
   100741 gccattctta gataatgttg atcgttatat cagtttatgg gaaacggcga tatcactgga
   100801 agaagatata tccgagaacg gcattagact ggagaatggt aaaaagaatg aatcagtagc
   100861 gttgcttgtt tctgtcaaca aacaaatggg attgatgttg gataaacttg ccattactcc
   100921 tgaattggta ggtgaagcaa atgaatcaat tcctgagtta taagcatatt gaaaattggt
   100981 tcaaagctat agaagaaggc actatcaagg tatgcaaaga gcaattattg ctaaaaaatt
   101041 atctagaaga aagagtcttt actagagaag atatttactt cgataagcag atggtagagg
   101101 attcaatcaa tataccagca caatactttc cattcgaatt aattccgtgg gaaaaatttc
   101161 tacaatgttt tatttatggt gttcgatgga aaaaagataa aacactagtg ttcaatagat
   101221 atctttcatt aatgggacgt ggtaatggta aaactggttt tgcttcttgg aacaacttct
   101281 ttctactaac cgctaaacac ggtattaaaa attatgatat tgatatctat gccaataatg
   101341 aaagccaagc aaagactagt tttgatgatg tatttaaagt aattaaagat catcctgatt
   101401 tagataaaaa agtatttaaa gctacgaagg aagttattca aaatatcgct acaaacagca
   101461 aacttcgtta taacacggca aatgctagaa caaaagatgg gaagcgacca ggtgcaaacc
   101521 gctttgatga aattcacgaa aatgaagatt attcaatgat aaatgtggct acttctggtg
   101581 gtggtaaaat tcgagattat agagaatttt atgatacaac taatggtcat gttcgtggtg
   101641 gtccgcttga tgacattata gaagaatcaa aaatgattct ttctggagaa cttggaattg
   101701 acaaggatgg agcagaattt tctagtttgt ttccatttat ttgtcgcttg gataacgata
   101761 atgaagttga tgatcccgac atgtgggaaa aagcttgtcc aactattaat tacaatgcag
   101821 atttaaaacg gaaaatgttt caagaatact ctcaaatgca acgtaatgct ggtttaagac
   101881 ttacgttcat gaccaaacga atgaacagac ctatggaaga tacacgattt gctgttgctt
   101941 catatgatga tgttctgcat acgaaagaaa aagaatttcc tgaaaaaatg gatgaagtga
   102001 taggaacagt cgattttgct gatagacgag attttgccag cgttgggttg ctaggaaaat
   102061 acgataaaga tgtttatttt acacaacata cttttatcca cgaatcagcc cttcgattac
   102121 aaaacatcaa acgagaggtt atagatattt ctatagatca aggaaaatca cagatcgttc
   102181 atggaaaaaa tatagaagct gattatattg taggttggtt tcttgaaatg agtaataaat
   102241 attatattaa aaaaatcgct atggatatgt accgtgcaaa aatattgaag cccgctttag
   102301 aagaagcagg ttttactgtg gaaattgttc gaagcggatc tgttacacat ggtatgttaa
   102361 aagatctggt tgatgacctt tttattaatc aacgtttatt ttttggtgac gatgcgatta
   102421 tgcgttggta ttgcatgaat gtatatgaag agcatatttc taatggaaat atacgctatg
   102481 aaaaaataga acctgaaact agaaaaacgg atggcttttt ttcattcctt catggtttga
   102541 attttttaga tgatatttat gattctgctc ctgtaacagt cacaaatagc tcagtagaaa
   102601 atacaggaac tggatttact cctctagtat tctaacttga aaggaggtga gaaagtgggg
   102661 atttttcaaa aggcggtagg atacttcaca aaaaaagcaa cggttccttt agaagaatac
   102721 ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg cgattgaaac ttgtattgat
   102781 ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat atgaagatgg aaaaaataaa
   102841 aagaatgatc tttactatag gctgaatgta gctcctaata agaaaaataa tgcaacagaa
   102901 tttagaaaaa aactgatcag gagattaata ttctacaatg aagtattgat cgtttctccg
   102961 tctaataatt ctagcgaaat atttattgcg gatagttggg atgtcacaga atatgcattg
   103021 aaagatgatg tgttttctca agtgcaaatt aacaacatag tccttgatag agaatttcta
   103081 gaaagtgatg ttatctatat aaaatacgca gatcaacaaa ttaggcaact agtcgatgcg
   103141 tattatcaag cgtatgggaa actcatttct agtgccatga atgtttacaa gcgctctaac
   103201 gctcgtagat acgtactgaa agggaattta ttccgaccgc aagacaatac aacacaagat
   103261 caaatcaata aaatgatgac atcacaattt aaggctttta tggaagctga taatgcaggt
   103321 gcggtatttc aattacaaaa tgagtacaca ttagaagatt tcagcggaaa cttccaaagc
   103381 aattcaagag atataaaaaa cttaatagac gacatctttg agatgacagc agcagcgttt
   103441 cacgttccga aaaacctact aaagggagac atgagtgggt tatcggatca agtggacgct
   103501 tttttaatgt tcgaaatcat accgattgct gaacttattc aggatgcgtt taacgctagt
   103561 ctctatgaag tagaagaata cttgtcaggg aattttgtac gtgtggatac aactatgatc
   103621 aagattacta gcttcaaaga tttggttgac gctattgatg taggcattag aaatggagta
   103681 tttacaatca acgaaggaag agaacgcgtt ggaaatgatc gctctgataa ggcgatggca
   103741 gatgaaatat ttataactaa aaacaatcaa caagtatcga aaggaggtga ggcgaatgac
   103801 gacaatgaaa acatttctag cagtaaagaa tgaaggcgca gtaccgcaaa tttttattca
   103861 gggatttatt ggttctagtt ggttctttga agggaatact gacaagggaa tcaaaaatat
   103921 tttggatagt ctaggtgatc aagaagaaat tgaagtagta attaattcaa acggtggaga
   103981 cgtatttcaa gggattgcta ttgggaactt acttaagtca aataaagcaa aagttaacgt
   104041 tgtgattaac ggcttagccg ctagtgctgc ttcaattatc gcaatggctg gcgatactat
   104101 aaaaatctac aacaatgcac aattgatgat tcaccgcgct tccacatacg gagaaggaaa
   104161 tgtcgatgac ttccgtacga ttgctgacca actggaatca attgataaat cggtaaaggc
   104221 ttcatataaa acacgattca atggcacaga tgaagcattg caagaacttc ttgaaaaaga
   104281 atcgtttatg gatgcagaaa cagctttgag ttatggattg gtcgatgaaa ttatcgatgc
   104341 agaaaatagc tcaggtactg aagctaaaaa agaacaaagc gttgaagaaa ttttgaatga
   104401 cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca gcagcattaa ataaaacatt
   104461 tggacaagga gatgtaaaat aatgacagtt aaaaatttaa aaggtgtaac agctgcaagc
   104521 gaccaattga tgaaagcttt taaagatggt aacgaagaat cttttagcgc agctatggta
   104581 agcttatcta aggaaattca ggataaaatt ttagaagaag caacagcaaa aaatcaagat
   104641 caattagtat taatgaaccg tggtcagcgt gtattaacta cacaagaaac aaaattctat
   104701 aacgaagtag tgaaaaacga aggttttgca ggggtcgaag aattagtgcc agctactgta
   104761 tttgaacgtg tatttgaaga tttagaacaa tctcatccac tattgcaaaa aattactttt
   104821 gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg gagtcaatcc agcatggtgg
   104881 ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg gctttgatgt aattaacatg
   104941 aagcagttca agctatcagg ttatattcct gtatgtaagg caatgcttga tttaggtcca
   105001 gtatggttag atcgttatgt ccgtactgtt ttagtagaat cattgagaat tgcattagaa
   105061 caagcaattg ttgatggtac tggtaaagat atgccagtcg gaatgatgcg tgacatgagc
   105121 aaacaaacta gcggagaata tgctgaaaaa aaagcagaac ctattacagc tttagatgct
   105181 gtaactatgg gcggtttgat ggcgcgacta tcaaaattca atatcgaagg tgtgaatgat
   105241 ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa ttgtgaatcc aactgattac
   105301 tggtctaaag tattcccagc taagactgta ctaactgcta atggagaata cgtacaagta
   105361 ttgccagtac cagtttcaga tttgcagtca acggctgtgc cagaaggaaa agcagttatt
   105421 ggggtagcct cagattactt catgggtgta ggatctacac taaaaattga agcttcagat
   105481 gaataccatt ttgttgaaga cgaacgcatt tatctagcta aacaatatgc aaacggtcaa
   105541 cctaaacgta acgatagttt cattgtatta gatattagcg ctttgggaac tactactaca
   105601 actacaaaac caacaaccac aacaactaca acacaagcgt aggtgatcag aatgaagtat
   105661 attctttgtc agccggcaat caatcggttt aaatgggagc ttgaagtttg tttaactaat
   105721 ctgaagaaac taggaatcaa agatatcgta ttgcttttca gcagacacga tgatcagatt
   105781 cctatttttt ttgagaagga atatggtgtt gaagttcatg tgtacgatga tctgcgggac
   105841 gacaaagagt atattccttc gattaaacca tatttatggt ggaaatattt agaagaagat
   105901 cattcgcgtg aggacgaccg atatttctat atcgattcgg atgtcatttt caataaaaga
   105961 attaatttgc gcaaattgcc ttctaaagat gatgtttggt attgtagcga ctgctgtagt
   106021 tatctaagtc ttgattatat tagaagctgt gaaaacggag aaaatattct aaaagatatg
   106081 gcaaacattg taaatgttac agtagaatct ttggaaacta taaacactaa ttcaggaggc
   106141 gcacagtggg ttattaaccg tcctaaagcg aattattgga aaaaggttta tctggattct
   106201 aatcggctat atcgctacct tagagggcaa aaaacaaata tacaaatctg gacagccgag
   106261 atgtgggcac agctttggaa catgatgtat ttcaatattg gtcctaaagt tcacgaggaa
   106321 ttagactttt gttttgctac tgatccaata gaaaaagtta aagaagtaaa aatcttacac
   106381 aatgctggag taacaacaaa cgatgaagat ttatttttca aagggagata cgtgacttct
   106441 acgccttttg atgaagattt atcatttgta aacaagaaaa aatgctctta cgcatatgtt
   106501 aaagcaatta aggcggtggt tagatgacgc ctgaacaagt gactgaagaa ttgctaatag
   106561 ctgtgaagga taatatttac gttacctgga acgaagaaga tgagtcaatt aaaaagatga
   106621 tagctaaaaa tgctgtttat cttcaaagta aagtgagtac aacactttct ttttctcctg
   106681 aaagcttaga atacggattg ctaatcgaaa gatgtagata cgactggaat cgtgctttag
   106741 atgagtttga acaaaatttc gctagtgagt tattaggttt cattcaacat tatgcgctac
   106801 aagaatatat tgcaggtgat gtgaatggcg aataatcgta gactcgaaga aacattcaac
   106861 gatggttggt taaagatttt gacgcaaact accaaaagaa atgaactagg aaaaaagatt
   106921 ggtgtagaag atacagaaat cacttcttta aaatttagaa atctttccat gagagatagt
   106981 gatataacag ctatggatgc gatgggatcg aaattaacta agaaagtaaa gactccattt
   107041 catccaatcg ccaagaaatt taataaagat caatatttta tcgtaatcga tagtatgcgt
   107101 tacaacgtta tctatgccga ttacgataat ttttatatct atttttatct tgaaagtgtg
   107161 ggtgaatatg gtgattgata attctaaaga aaaagaacgt ttaaataagc aaatttctgc
   107221 tatcaaaact tccttagaag agcattttgg cctcaaactc tttcaagact ccgtaggcga
   107281 ggatgagcta cctgatgatt ttaattactt cattctcgaa acaggagaaa tagaaatgat
   107341 cactgagcca aaatatagcg tgggtcaaaa tctatatcta actttctatt cagaaaatag
   107401 agaagattta acaggagact cactagatat tatttcattg attcaaaatc gttcgattcg
   107461 ttttcagaga atggatccca atcatttaaa actagagaac caagatcgct atatcgatca
   107521 attggtattt acgtttagac gattattgaa gagtgattgt catggctaaa aatagttggg
   107581 agctaaaaat aaatggacat gatgaacttc ttgtgcggat ggaacgctat tcaagcgaga
   107641 gcgaacgact gattaacgaa gcattgaaat caaaaggttc ggctattgca gtggatagga
   107701 ttacagaaaa aattcctgtt tctgaagcag atttaagaag agggcaccaa cacgcaaaaa
   107761 atagtcgtcc acttaagact caatacatta atttgggttt catcattaga cctacaagaa
   107821 aatttgagta tttaaaatat cctgatttgg ggataggtac ttctaaaaga aatcagccgg
   107881 acgaatttat gagaagagga ttaggtcttg cacttgatcc aattacagaa cttctgattc
   107941 gtcaattcga taaattaaat aaataggggg aacaacaatg gctaaaacaa aaactgtagt
   108001 aacaacgttc gataacgtaa gtatcaaacg aattgctttt aattttaaga acgcaggaaa
   108061 tgcaatcgca acgaattgta acggacaatt agatggcgaa acagaaatgc aaacggtggt
   108121 taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa ccaatcaata tgacggtaac
   108181 aattactgca catgtaccga tggaagttta tcgacgtttc aatgggttga aacaagatgg
   108241 acgtattaaa ccaggcattt actcttacgg tcctgattcc gtaggcgaag atttctcact
   108301 tgctgcagag atcgtggatg acttcgaaga aaatagaaag ttagttggta tgttagcatg
   108361 cacttcgaat acaggattaa cattctctat tgaaaatggt gcggatgaag tagctgcgtt
   108421 agaactagaa acaaaagtta tgcaagatga atttggtaaa ttttatcatg aagcaattgt
   108481 tgcagaactt gaagaagact taacagatca atggatgacg aatctatctg ctgatgtgat
   108541 taaaaaaact tcagttgtga caactacggc cactcaatca cagtaaaaaa aacggaggta
   108601 gcgaaatgaa cgaagattac tcaaaaattg aactaaacga tggaacaatt ttgaatttag
   108661 aacctaaact gaatatcaag aaattattga tgatcaatag agattttaac acagacgagt
   108721 ttgcaaaaat gactgtggga aaaggatcca tggatatttc tgttattcaa ggtgcaaagg
   108781 ctgtgtatat tgcttaccgc caagcgaaca tgactgatta tatttcattc gatgaattta
   108841 tcgataaatg ggattttgat atggctactg ccagctatat ttatcaattg atgatgttca
   108901 aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc aaataaggaa aaaaagcttc
   108961 aaaagtaaaa attccaaagc tcttagttga aacgtgggtc gatgtctatt cgatgttgac
   109021 cgacgttttt tctatgcctt cagatttggt tttaagcgat atctgtttag atgacatttt
   109081 gcaaatggct tacaacaaga gtgcttatga aggatggaaa aactatgcaa taaaccaatc
   109141 ccagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa gagaacagaa gcagaagtaa
   109201 ctttcatagc taacgatgac ggattgaaat ctacgttaaa agaaatcagt gctgaattaa
   109261 ctaaaaatag agcagaatta aaactagaac aagctcaatt acaacagact ggttctgaat
   109321 cagacaagtt aggaagtaaa ttatcttctt tagaaaagca gtatgaatta caaagtcaaa
   109381 aagttgaagt aactagccaa cgtttagcca atgcgaaaaa atattatgga gaaaattcca
   109441 ccgaagttaa gaaacttgaa agagaactga ttaatcaaca aacagcgcaa caacgtttgt
   109501 caaacgaaat tgataaaacg agtaatgcac tagctcaagc aaaaggcgaa atacagacgt
   109561 acgagtctac aatgcaacag ttggatagcg aacaaaaaaa tgttcaagct agtgcttctc
   109621 tgattgaatc cgaatacaaa aaatggcaag caactgctgg tcaatcagct tctgaagccg
   109681 agaaattagc gaaagcccaa gaatatgttt ctcaacaatc tgaaaatgcg gagaaaacga
   109741 tagatatcct aagacgacag ttagaagcta cacagtctga atttggcgct acatccacag
   109801 aagcaatgca gatggaagcg aagcttaatg atgctgaacg tgaatttgaa gagttaggac
   109861 aagctgctaa aaatgtagat acaactaact tggacgatat cggaagcaaa atagatatga
   109921 ataatttaat ggaagcttct gacgttttaa gcgacattgg cgataagctt acagaattag
   109981 ggaaacaagc agtggactct gctaacagtg taggtagttc ccagagtaag atacaagcta
   110041 attttggttt gtctaaacaa gaggctgaag aattaacgaa tgtagccaga gacatttatt
   110101 ataaaggttt tggagaatcg ttagatcagt ccacagatgc attgattttg gtaaagcgta
   110161 atttaggaga tttaaataat caagatttac aaaatatcac ggaacaagct atggtcctag
   110221 aaaacaccat gggcgctgat atggatgaaa cgttacgtgg tgtaaatggc ttaatggtca
   110281 atttcggctt gagtgctcaa gatgcaatgg atttaatggt ttcgggtact caaaacggtt
   110341 tagataaaac gcacgaatta ggcgacaata tggcagaata tagccaatta tggagtcaaa
   110401 tgggatattc agctgatgaa acgttcggaa tgcttcaaaa tggtttagat gcgggtgctt
   110461 ataaccttga taaagtcaat gacttagtta aggaaatggg aatatcgtta acagatggtc
   110521 gatttgagca aaacatggat atgtttagtg aaagtactag aaaagctttt gaagagtgga
   110581 aaaatggcgg aggaacacaa aaagacgtta ttaattccat gattcaagat tttagcaata
   110641 tggatggtca atacgaccaa ttaaataaag cttcgacaat ttggtctgcg cttggcgaag
   110701 ataatgcgat gaaagttgtc caatctttaa ctgatgttaa ccatacattt gatgatgtta
   110761 gtggatctgc acaaaaaatg aatgaagatt ctactactcc gttgcaagag ttgaacggga
   110821 aaatagctga attaaaggat tcattagctc ctataggcaa cacaatcata gatgcactcg
   110881 aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt taataatctt ccacaaccag
   110941 tacaggattt cattgttgct ataggtggtc ttacagcagc atttggcata ctgttaccgg
   111001 caatactagc cgtgtcatat ttatttggtc cgatgatgct tataattgga ggaattatag
   111061 ctgttatagc aggcgttatt gtggcaatta agaactgggg tgcaattact gactggttta
   111121 gtggtttatg gaaaaaattt actgattggt tgggtgatac ttgggaaagt ataaaagacg
   111181 gagcctcatc agtttgggat ggggttaaag aaacctggtc tggatttgta gattgggttc
   111241 aagatatttg gcaaggagtt tctgattggt ttggagagct atggagcgga ttagttgaag
   111301 gagcttccaa catctggcaa ggagtccaag aaacttggca aacattcgtt gattgggttt
   111361 caaatatttg gaacggagtc aaagaagtat ggtcgattat ttgggcagac attgtaggaa
   111421 ttgttcaaat accatggaca ttaataacgt cattgattca agctggtatt aatattatcg
   111481 tgggtatttt tgatgtagct ggacagtgat tgggcgcagc ttggcaagct gtttggacac
   111541 ctatttctga tttccttaaa aatacttggg atactatgac acaatggata agtatcgctt
   111601 ggaatgggat tgtaactaca ttccatacta tatttgatcc agtagtggca tggtggaatg
   111661 gtatatggac agctattagt actacggctt caaatatttg gaattcaatt agtgcaacag
   111721 cttctagtat ttggaacagt atcaagaata caatcactag cttggtacaa gcagctgcta
   111781 cagtaattca aaatatttgg tcaactgtat ctagttggtt aggtggaatt tggaattcaa
   111841 tcagctctac agcatcaaat atctggaata gtgtgactag tagtataagc aatgctataa
   111901 acgcagctaa aagtgccatt caaagtgttt ggaatagtat atcttcgtgg attagcggaa
   111961 tttggaacgg tatcaaaaat actgctttga atctttggaa tggaattaca agcactatta
   112021 gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg ttggtccaat ctaacaggta
   112081 ttgtttccga catattcaat aatgttaaaa gtacaattgc taacatatgg gaaggcatca
   112141 aaaagactgt tagcgctcca attgattgga ttagagacaa aatcagtggc atttttgata
   112201 atttgaatat ttctatacca catattccgt taccggaatt tatcatggaa ggcagcttta
   112261 acccgctaaa aggtcaaatc ccccatttgc gtgtaaaatg gcatgctaaa ggaggtatct
   112321 ttactaaacc aactttacta ggtggaatga acggtgtcgg tgaagcagga cctgaagcag
   112381 ttttaccttt gaaaagatct gttttgcaag aaattggtga tcgtatcttg agtagcacat
   112441 cagtttcatc tagggcacaa acgattcaac ctgtgaataa ctacgaattc aatttcacaa
   112501 ttgatggtaa cgcagatgag gttactatga agcaaacaac tcaacaaatc attgatagca
   112561 ttacaaaaat tcaaaatgat aatgcttcgg catggcgtta aacaggagag tatttctcct
   112621 gtttttttag tattaaaaag gatgtgaaaa aatgactgat tgtatacatt ctataatcga
   112681 tggatttcct gattatttgc ataaattggc tttagctgaa agaccaacca taccttctcc
   112741 aaaaagacag agagttgaaa cttctgtttt aggtaggtta ggtggcttag tacaagatta
   112801 ctcgtttgaa gatatgtcgt ttacattgca ctataactat ttagaggatg tggaagacca
   112861 tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg ttaaactatg caaagaaatt
   112921 agaattctct gacgatccca acgtctatta cgttatccag actatcgata ttggggatgc
   112981 agaaaacgat attgttgaat ggggagagtt cgatgtaaat attactgcga aaccattcgc
   113041 aagagttcaa gaagatgtac ctataaccgt agataaacca cagtcattta acttgctgaa
   113101 taatagttta gaagaaagtt ttccaaagat tatcatcact ccttcagcta cttcatgcca
   113161 gttcatctta aatgattatg tgtttagttt cgaaggctta gtagcaggaa ctgatgtagt
   113221 cattgatagt gatttgatgc tttgctacga agagcaatcg gacggagata ttttagatcg
   113281 gtccaacaaa atgaagacca tgcaatatcc gacattgcaa gtggatatca atcattttaa
   113341 ttgcactggt ttgagcaaaa tacaaattta tcgtaacggg ttaaggtagg tgaaatagat
   113401 gatcgataat ttaataacta tttacgataa aaatgacgcg aataatttag ctgaacattt
   113461 atatgatacg caaggtttag gcgctttgtc agactggtta acagctactg ttagcaataa
   113521 actaaacgga gccgagatat ttcagggtac ttattcaata aacggaacta atgcagattt
   113581 gattgtagac ggacgtatta ttcagtgtta tgtagatgaa aatcgagcaa aacagcgtct
   113641 acggatttat tatgcaaaaa cttctgtaat aggcaatacg atagaagtaa aagctgaacc
   113701 tattttcaat gatataagaa aatcggtgtt gaataaatat gacagcggaa cagaaaagat
   113761 cactgctact caggcatggc aaaacgcaaa agttttagcg aaaccagcta tcccttcgca
   113821 gttttctttc tcgtcattag tagatacgct tgctaatgtg aagatagaaa aggcgaattt
   113881 tttagaattc tttggtggaa aagagggatc tattctagat cgatttcatg gtgagtttct
   113941 aaaagataat aacacattac gtcatgaaaa aagtctaggt acggatcata aaatcaaagc
   114001 gatttatact aaaaacttaa ctggtcttga cttagagatt gatgctcaaa gtgttttagt
   114061 tggagtttat ccattcatta gcagctcttc agaaggagaa gacgagatca ctctaccaga
   114121 agaagttatt ttcacggatt acgtggatga ttatcctgct ggatatgttt cttttgttga
   114181 ttttaaagac aaagcgactg atgtagccac attaagggaa gctgctaaag actggttgaa
   114241 aacaaacata gataaacaaa aaccacaagt gagtggttcg attgaattag taccattgag
   114301 gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa gtttcgatgg gtgacggagt
   114361 agatgtgtat catccacagt taaaagtgaa tatgtcagcg agaattgtgg aatatacgtt
   114421 taatgttcta accaattcat acgataaatt agttgtagga aacgtcaaaa caaacttctt
   114481 agaaaacaca gagaataatg tcagcaattt gattaatgat gccattgatc aattgaaaaa
   114541 cggtggcgaa atcagtgatt tactcaatga tattgtagat catcaaactg atatgattac
   114601 tggtcaaaat ggtggttatg ttttattaga tcctaaagaa gcgcctagtc gtattttgat
   114661 tatggacaca ccagataaga ataccgcaag gaatgtttta caaatcaaca atgctggtat
   114721 tggtttttct aaaactggca ttaatggaac atatgaaacg gcatggacgt tagatggcgg
   114781 attcaatgcc tcgtttatta cggctggtga aatagtagga attactatta gaggtactac
   114841 attaattagt gatggtgctg attatagaac aagtattgct aatggcaaaa tgacttggta
   114901 ctcaaaaaaa gttaacaaag atattatgga gctagaagca cgtgattatg taagtgctga
   114961 tgccggtatt gtatcataca ccatgaaaac tggtggtggt ttcatgatta gaaatccaca
   115021 gggtaacttg gtttttagta cgtgggataa tggtaataac agaccgtttc tatcttttgg
   115081 tgcgcccaat ttcaggtata gcaatgctag ttatgtaact tctggcgacg gtagttcttt
   115141 aagcattaat ggtagtgcgg gtaactcatg ggaatttaag gtagctggta ggactatgaa
   115201 atttactagt gatggtatgc taacgttacc aggttgtttt tttggttcat gggaagatgg
   115261 gaaacttgct aggtttgaac aatcaacggt acaagtatat aaagatttta ctgttagagg
   115321 tactaaaaac tcaactgtac caacagaaca ttatggacaa cgactattga acgcttatga
   115381 aactccagaa tattatttcg ctgattatgg ggaagccgtt acaggtgaca atggtaaagt
   115441 tcgtgttgat attgacccca tgtttgctga aacagtaaat ctaagtcggt atatgacaca
   115501 tgtgacacct acagaactag ttttgtgtgc tgttactcat gaagatattg accatttcat
   115561 cattgaaact agtaagccaa acgtattagt tagatggaat ttagtggcac accgtctagg
   115621 gtatgaagat attagattaa aagaggatac agcatatgat agcacagtgc ttgaccaaaa
   115681 acgtttttaa aacgaagaca aggaggtata taaatggcta gcagtttata taatttggct
   115741 ttagatttca gcaaagaatt aaactacacc aaagctatta tggctcgtca aggtgataaa
   115801 gggattacgg tgacggttaa accgtttcta aatggcttgc agatggatac gagtggcgga
   115861 acatttactt taaaaggaac aacaccatct aaccgttacg tagataatgt tgcaactagt
   115921 gtaactagtg aagaagtcac gttttctctt gatggcacat ttatgagtga agcaggatat
   115981 tataaacact gctacgtaga atatagaaaa gacaatcaaa ttttaacaac gcaagatatc
   116041 atttttttct cactaggagt gtctgacatt tcgcaaggcc aagccgatga atatgtttcg
   116101 caattagaag agttgattcg aaagtacaac gaaacttttg atgcttttat ggctgaaatc
   116161 aaaggtagag tggatagctt aaatcaacag attactgatt taactggtca agctaaaacg
   116221 ctacaagaca agttagatgc tctgaaagaa gaaatttcta agttaggtaa cttacaagtg
   116281 atgtacagta acagcatcga cttcggggac tatgattatt cggagaaccc gaatttaatg
   116341 ccatatatta cagaaccttg ggttggacct ttgttaggta atggacatac agttaaagat
   116401 agtgttaaaa gggttataac tcatactaaa actagaacag ctaattccgg cgacatatta
   116461 tcgcttggct taggtattcc gtgtacagcg gaagctaata ataggtatct tataaccact
   116521 ttacgaccat ctacgacata cacattaagc gttactatga gtgtcggttc agattggact
   116581 ggagagacca atactatagg ggttaggctt agatatttaa atgaacaagg tggtatagaa
   116641 ctgcccatta atgcattaat accagcaaat gtagaacgcg ataaaatggt cactcataca
   116701 tttactggta taacaaaaga taatgtaact agcataacaa actgttatgt tgaaatattc
   116761 tcacttaata gtgaatataa aggaacggtt agtgtcagct atgacgttaa gttaaaagca
   116821 cactacccta acttactaga tggcccttac tggttaggta aagttccttt aggcgagaat
   116881 attgctgacc ctacagttgt ctttccacac aaaactagtg aatatatggt ttatggtaga
   116941 cgcaatacag aaaactatat cgctgaccaa acatatacca tatcaatgaa agcaactaaa
   117001 ctaacagttc aatcgtttgc cgtgtatatt gctgctggcc gcgttaaagt aggagacatg
   117061 aagcctactg aggggttagc taatacatgg gagttaactt ttactgtaac taagcaacat
   117121 atagatagtg gggtaactaa ttatctggaa atataccaat acccgtcagc tacaaaagga
   117181 gctgttcaaa ttgagtggct caaactagaa aaaggcaata ctcgaacccc gaatattagt
   117241 gagtataaat atcgtggtac tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg
   117301 gatctagcac cagaatatgt cgaagataat ttggccacag atattaaaat ttctgaaatt
   117361 actggtaaag caaacaatta taccgatggg aaagtatcgg agattaattc gcagttgact
   117421 gcttcaatta atgaagtaga caccacagct aaggatgctc aaacaaaagc gaatgctaat
   117481 gcgactgcta tagatgaatt agacaataag atcgatgaac gcattaatga tacagctact
   117541 accacattaa cagttacaaa cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa
   117601 acagtttcta tatattttgt ggctttaaac ggaaaaagca gtggtggaaa tgattcaacg
   117661 atactaacaa ttccagaagg ctatcggcca ccaattagtt ttgagcaact ggttggctcg
   117721 atagaccgtt ctactttgaa cagtgctcag ttatctattg gtgcagatgg agccattaaa
   117781 tggcgaagaa actcaagtta tggatcggat tatacctttg caattactta cacgatttag
   117841 aaaagcgtga atcgatatga aggcagcata tagaccaatt gaaccttacg gattcgagca
   117901 aatcattgtg aatgatgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat
   117961 tccaaattgg aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa
   118021 tatggacgta ctggcggaga gtacgcagga aaagtattgg acgacatgct tgtatccgtt
   118081 ccttacacag atcagccacc gcttgaagga attcgtgctg atggcgaacc gttaacaatc
   118141 gctgatcaaa tgtttgatcc taaattgaat caatggatta ttttagcgaa cgcactagat
   118201 cacaacgatt taaacaatct caaagcaatg tatgagtcgt tagaaaatga gaacggcgat
   118261 ttaaaacaga tcaatgccaa actcatgcta agcgatgtag caattaaaca ggaaaatact
   118321 gcattgaaag aaaaagcgga tagtttagca caaatcaatt caaaaatgat gcttgcttcg
   118381 ttacaaaata gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaggga
   118441 ggtgagtagt atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgtttcac
   118501 taacgaacaa gtaatggttt tcgttccgtt gtgcattact gaagaaaaag cagataaaat
   118561 cattagcaaa gaagagagcg catcttaatt gatgtgcttt ttattttgat tcaaggagtt
   118621 gtcacatgat taatttaggg gaatggggag cgatagcagg atcaataacc gctatcgttt
   118681 ctttgatttt attagtaata aaaccaatta ctgcatcttt ctcgaagatt actgagactc
   118741 tttcaaaagt aagtcacaat ttagatttgc tgactaaaga tttagaatcg agcaaatcag
   118801 atcgattgat gattcatgaa gaactaaaga aacacgatga aagattagat acacatgcag
   118861 aaaaattggt agaacacacg caacaaatta aaactttatt tagggagaga agaaaatgaa
   118921 taataaaacg ttcgaagtac taaaatggtt cgcactggta attattcccg cactagctac
   118981 tttcgtgggg ttagttggta aagcgctcaa ttggcagtac acagatatct gtgttgtcat
   119041 cattactggt tttggcgcgt ttttagggag tgtgttgggt gtatcaaatc gaacctacaa
   119101 aatgttctcg gctgaaagcg aagaaggagg aaacaaatga aaaagaaaat tactattact
   119161 gcgatgagcc tgttaacggc tcttttttta ttacccatta atacgtttgc ttatactatt
   119221 aatgacgagt ataatttagc gccgaatcaa ggagactcca gattagcaat tcctaacaaa
   119281 attattttgc acgaaactgg aatagatgca ccagcaagaa acgtagccgc caacatgaaa
   119341 aataattata acggaagcaa tccttatact acagatgtta ttggtgacgg tgggattgtt
   119401 taccgtgtgg gtgagcaagg atatgtttcg tggggagctg gtaacgctaa tccttatgcg
   119461 cctgtacaga ttgaattaca gcgcacatat gataaagcat tgtttgaaaa aaactatcga
   119521 gcttatattg aatatacaag agatagtgca aaaaaatatg gaattccatt gactcttgat
   119581 caaggaactt ctttatttac aaaaggaatc atttctcatt tgtgggtgac aaattatgtt
   119641 tgggggaacc acacagatcc atatggttac ttatcgcaaa tgggagttag caaagaaaag
   119701 cttgcttatg atttagctca tggatttacc gatgaaaatc caacaacttc tgaaaacaag
   119761 cctgtcattg atccaacacg agctggtgca gctaatccta cgctgacaga tggaacaaat
   119821 cacgcccaca ttgatcagtt tggggaaatc gaaaacgcaa acttgcatgt ggctggatgg
   119881 cacattgcta actataaata cgagtatatt ttcattatgg actacaatac tgggaaagaa
   119941 ttagctcgag taagagctga tggaatttat agaccagatg taaaccaagc ttataatact
   120001 tctggaaatg ttggttatca tgtatctttc aatatgcgta attttcctag taagaaagtc
   120061 tatgt
//
LOCUS       DACDYM010000002        92617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDYM010000002 DACDYM010000000
VERSION     DACDYM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.002"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(19..729)
                     /locus_tag="IWM59_000141"
     CDS             complement(19..729)
                     /locus_tag="IWM59_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9978207.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="IWM59_000142"
     CDS             complement(726..2198)
                     /locus_tag="IWM59_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9978208.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="IWM59_000143"
     CDS             2336..2809
                     /locus_tag="IWM59_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9978209.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="IWM59_000144"
     CDS             complement(2962..3513)
                     /locus_tag="IWM59_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9978210.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="IWM59_000145"
     CDS             3618..4148
                     /locus_tag="IWM59_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9978211.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="IWM59_000146"
     CDS             complement(4631..5074)
                     /locus_tag="IWM59_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9978212.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="IWM59_000147"
     CDS             complement(5090..6250)
                     /locus_tag="IWM59_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9978213.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="IWM59_000148"
     CDS             6757..8457
                     /locus_tag="IWM59_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978214.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLHA
                     "
     gene            complement(8559..9392)
                     /locus_tag="IWM59_000149"
     CDS             complement(8559..9392)
                     /locus_tag="IWM59_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9978215.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="IWM59_000150"
     CDS             complement(9696..11861)
                     /locus_tag="IWM59_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9978216.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="IWM59_000151"
     CDS             12121..14415
                     /locus_tag="IWM59_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9978217.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="IWM59_000152"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="IWM59_000152"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9978218.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="IWM59_000153"
     CDS             15066..16073
                     /locus_tag="IWM59_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9978219.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="IWM59_000154"
     CDS             16095..17114
                     /locus_tag="IWM59_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9978220.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IWM59_000155"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IWM59_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9978221.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /locus_tag="IWM59_000156"
     CDS             complement(18871..20292)
                     /locus_tag="IWM59_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9978222.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="IWM59_000157"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="IWM59_000157"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9978223.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /locus_tag="IWM59_000158"
     CDS             complement(22703..23959)
                     /locus_tag="IWM59_000158"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9978224.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            24539..25840
                     /locus_tag="IWM59_000159"
     CDS             24539..25840
                     /locus_tag="IWM59_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9978225.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="IWM59_000160"
     CDS             complement(25909..26508)
                     /locus_tag="IWM59_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9978226.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26763..27644
                     /locus_tag="IWM59_000161"
     CDS             26763..27644
                     /locus_tag="IWM59_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978227.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27657..29267
                     /locus_tag="IWM59_000162"
     CDS             27657..29267
                     /locus_tag="IWM59_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9978228.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAKGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29344..30003)
                     /locus_tag="IWM59_000163"
     CDS             complement(29344..30003)
                     /locus_tag="IWM59_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9978229.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30286..30951)
                     /locus_tag="IWM59_000164"
     CDS             complement(30286..30951)
                     /locus_tag="IWM59_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978230.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(31098..31703)
                     /gene="dhaL"
                     /locus_tag="IWM59_000165"
     CDS             complement(31098..31703)
                     /gene="dhaL"
                     /locus_tag="IWM59_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9978231.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(31731..32714)
                     /gene="dhaK"
                     /locus_tag="IWM59_000166"
     CDS             complement(31731..32714)
                     /gene="dhaK"
                     /locus_tag="IWM59_000166"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9978232.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(32716..33111)
                     /gene="dhaM"
                     /locus_tag="IWM59_000167"
     CDS             complement(32716..33111)
                     /gene="dhaM"
                     /locus_tag="IWM59_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9978233.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            33265..33585
                     /locus_tag="IWM59_000168"
     CDS             33265..33585
                     /locus_tag="IWM59_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9978234.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(33697..34566)
                     /locus_tag="IWM59_000169"
     CDS             complement(33697..34566)
                     /locus_tag="IWM59_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9978235.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(34662..35966)
                     /locus_tag="IWM59_000170"
     CDS             complement(34662..35966)
                     /locus_tag="IWM59_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9978236.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(35963..36472)
                     /locus_tag="IWM59_000171"
     CDS             complement(35963..36472)
                     /locus_tag="IWM59_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978237.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(36647..38782)
                     /locus_tag="IWM59_000172"
     CDS             complement(36647..38782)
                     /locus_tag="IWM59_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9978238.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(38865..40520)
                     /locus_tag="IWM59_000173"
     CDS             complement(38865..40520)
                     /locus_tag="IWM59_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9978239.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(40522..40836)
                     /locus_tag="IWM59_000174"
     CDS             complement(40522..40836)
                     /locus_tag="IWM59_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9978240.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            41006..41266
                     /locus_tag="IWM59_000175"
     CDS             41006..41266
                     /locus_tag="IWM59_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9978241.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            41280..41594
                     /locus_tag="IWM59_000176"
     CDS             41280..41594
                     /locus_tag="IWM59_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9978242.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(42324..42878)
                     /locus_tag="IWM59_000177"
     CDS             complement(42324..42878)
                     /locus_tag="IWM59_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9978243.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(43004..44933)
                     /locus_tag="IWM59_000178"
                     /pseudo
     CDS             complement(43004..44933)
                     /locus_tag="IWM59_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(45239..45955)
                     /locus_tag="IWM59_000179"
     CDS             complement(45239..45955)
                     /locus_tag="IWM59_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9978244.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(45956..47779)
                     /gene="glpO"
                     /locus_tag="IWM59_000180"
     CDS             complement(45956..47779)
                     /gene="glpO"
                     /locus_tag="IWM59_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9978245.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(47793..49289)
                     /gene="glpK"
                     /locus_tag="IWM59_000181"
     CDS             complement(47793..49289)
                     /gene="glpK"
                     /locus_tag="IWM59_000181"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9978246.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(49425..50885)
                     /locus_tag="IWM59_000182"
     CDS             complement(49425..50885)
                     /locus_tag="IWM59_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9978247.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(50875..52170)
                     /locus_tag="IWM59_000183"
     CDS             complement(50875..52170)
                     /locus_tag="IWM59_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9978248.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(52502..53179)
                     /locus_tag="IWM59_000184"
     CDS             complement(52502..53179)
                     /locus_tag="IWM59_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9978249.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(53209..54300)
                     /locus_tag="IWM59_000185"
     CDS             complement(53209..54300)
                     /locus_tag="IWM59_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9978250.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(54401..54835)
                     /locus_tag="IWM59_000186"
     CDS             complement(54401..54835)
                     /locus_tag="IWM59_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9978251.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(55419..56876)
                     /gene="ascB"
                     /locus_tag="IWM59_000187"
     CDS             complement(55419..56876)
                     /gene="ascB"
                     /locus_tag="IWM59_000187"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9978252.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(56873..58768)
                     /locus_tag="IWM59_000188"
     CDS             complement(56873..58768)
                     /locus_tag="IWM59_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9978253.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(58826..60601)
                     /locus_tag="IWM59_000189"
     CDS             complement(58826..60601)
                     /locus_tag="IWM59_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9978254.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(60651..62147)
                     /locus_tag="IWM59_000190"
     CDS             complement(60651..62147)
                     /locus_tag="IWM59_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9978255.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(62341..63963)
                     /locus_tag="IWM59_000191"
     CDS             complement(62341..63963)
                     /locus_tag="IWM59_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9978256.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(64291..65604)
                     /locus_tag="IWM59_000192"
     CDS             complement(64291..65604)
                     /locus_tag="IWM59_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9978257.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(65849..66073)
                     /locus_tag="IWM59_000193"
     CDS             complement(65849..66073)
                     /locus_tag="IWM59_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978258.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(66093..66461)
                     /locus_tag="IWM59_000194"
     CDS             complement(66093..66461)
                     /locus_tag="IWM59_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9978259.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(66657..67367)
                     /locus_tag="IWM59_000195"
     CDS             complement(66657..67367)
                     /locus_tag="IWM59_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978260.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(67385..67882)
                     /locus_tag="IWM59_000196"
     CDS             complement(67385..67882)
                     /locus_tag="IWM59_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9978261.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(67919..68371)
                     /locus_tag="IWM59_000197"
     CDS             complement(67919..68371)
                     /locus_tag="IWM59_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9978262.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(68506..69111)
                     /locus_tag="IWM59_000198"
     CDS             complement(68506..69111)
                     /locus_tag="IWM59_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9978263.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(69561..70181)
                     /locus_tag="IWM59_000199"
     CDS             complement(69561..70181)
                     /locus_tag="IWM59_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9978264.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(70183..71052)
                     /locus_tag="IWM59_000200"
     CDS             complement(70183..71052)
                     /locus_tag="IWM59_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9978265.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(71075..71482)
                     /locus_tag="IWM59_000201"
     CDS             complement(71075..71482)
                     /locus_tag="IWM59_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9978266.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(71501..72790)
                     /locus_tag="IWM59_000202"
     CDS             complement(71501..72790)
                     /locus_tag="IWM59_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9978267.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(72817..73119)
                     /locus_tag="IWM59_000203"
     CDS             complement(72817..73119)
                     /locus_tag="IWM59_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9978268.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(73144..75237)
                     /locus_tag="IWM59_000204"
     CDS             complement(73144..75237)
                     /locus_tag="IWM59_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9978269.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(75726..76490)
                     /locus_tag="IWM59_000205"
     CDS             complement(75726..76490)
                     /locus_tag="IWM59_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9978270.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(76601..77470)
                     /gene="mdcB"
                     /locus_tag="IWM59_000206"
     CDS             complement(76601..77470)
                     /gene="mdcB"
                     /locus_tag="IWM59_000206"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9978271.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(77495..78571)
                     /locus_tag="IWM59_000207"
     CDS             complement(77495..78571)
                     /locus_tag="IWM59_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9978272.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(78595..79188)
                     /locus_tag="IWM59_000208"
     CDS             complement(78595..79188)
                     /locus_tag="IWM59_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9978273.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(79192..80868)
                     /gene="mdcD"
                     /locus_tag="IWM59_000209"
     CDS             complement(79192..80868)
                     /gene="mdcD"
                     /locus_tag="IWM59_000209"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9978274.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(80858..81157)
                     /locus_tag="IWM59_000210"
     CDS             complement(80858..81157)
                     /locus_tag="IWM59_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9978275.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(81259..82905)
                     /gene="mdcA"
                     /locus_tag="IWM59_000211"
     CDS             complement(81259..82905)
                     /gene="mdcA"
                     /locus_tag="IWM59_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9978276.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(82912..83823)
                     /locus_tag="IWM59_000212"
     CDS             complement(82912..83823)
                     /locus_tag="IWM59_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9978277.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            83943..84800
                     /locus_tag="IWM59_000213"
     CDS             83943..84800
                     /locus_tag="IWM59_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9978278.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(84987..85349)
                     /locus_tag="IWM59_000214"
     CDS             complement(84987..85349)
                     /locus_tag="IWM59_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978279.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            85695..87134
                     /locus_tag="IWM59_000215"
     CDS             85695..87134
                     /locus_tag="IWM59_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9978280.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(87141..89693)
                     /locus_tag="IWM59_000216"
     CDS             complement(87141..89693)
                     /locus_tag="IWM59_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9978281.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(89807..91138)
                     /locus_tag="IWM59_000217"
     CDS             complement(89807..91138)
                     /locus_tag="IWM59_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9978282.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(91104..92582)
                     /locus_tag="IWM59_000218"
     CDS             complement(91104..92582)
                     /locus_tag="IWM59_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9978283.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
BASE COUNT        26843 a        19546 c        16068 g        30160 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttaca tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcatg tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    26641 tataaaaaga atattatgta ctaaaataat aaaaaaaagt tttataaaaa ggtataaaac
    26701 ctttttatta aactatttaa tcttgtttag tatatatttt tagaaaaagg agtggggttt
    26761 ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag ctttggtcgt ttcgaagctt
    26821 taaaagatgt ctctttcacc gtacattcag gtgaagtcgt tggatttatc ggaccaaatg
    26881 gttctggaaa atccacgacg attcggacac ttttagggat tataaaaaaa gacggcggaa
    26941 cggcaacgat ttttgggcaa gatgtatggg aagatagttt atctatccat gaacgtcttt
    27001 cttatgttcc aggcgatatc tctttgtggg gaaatttgac tggaggagcg atcatcgatc
    27061 tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa agatgaactg attcaacgtt
    27121 tctctcttga tcctaagaaa aaagcaaaaa actattcaaa aggaaatcgc caaaaagtgg
    27181 gcttgattgc tgctttggct gttgattcgg atctctattt atttgatgag cctacttctg
    27241 gtttagatcc tttgatggaa acgattttcc aagaagaagt tgaaaaattg aaagaacagg
    27301 gcaaatccat cctactttct tctcatattc tcagtgaagt cgagcgacta gcggataaag
    27361 tcgtgatcat acgcgaagga acactcgatg aagaaggaac actcgatgaa ttgcgtcatt
    27421 tgactcgttc gaccatcact atagaaacag aagcagagaa tagtcactta gcacaattga
    27481 acggtgtcca tgactttgtg caaaaaggtc acctcgctca atttgctgct gataacgaag
    27541 ctttcgatgt gattttagct gaagcgtcca aattaggtgt gaaaaaattt gaatctattc
    27601 caccgactct tgaggattta ttcatgagac attatgaagg atgaggagga agcgaaatga
    27661 ctgataaatt ttcacgctgg tccacccttt tttctcaaaa ccttagccgt gactggaaaa
    27721 agatcctgat atggctagtc ggtgtcactg ctttttctgg tggttttgtc cctgcgtttg
    27781 aagaattgac ttccggacaa ggattagctg gattatacga aaccttgcaa aacccggcaa
    27841 tgattgcaat ggtcggacca actccagctt cgaatgctgc tgagtatact ttaggagcga
    27901 tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc tgctgtgatc tctgttttac
    27961 acgtggtgag ccaaacgaga aaaaaagaag aacttggtct gattgaactt attcgttcct
    28021 ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc tgaaagtatt ctgatcaatt
    28081 tgcttttagc aatatcgatc agctgtacga tgattttttt ccaagcggat tcgatcactg
    28141 caaaaggtgc ttggcttttt gggctatctg taggtagtat ggggattttg gggagtgccg
    28201 ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag ttcgaatggt ctgtctttag
    28261 cgtttttagg tggactttac cttctacgag caataacaga tgtagctgat tcctcccttt
    28321 cgatgtggaa tcctttaggc tggtcttatt tgacctatcc atttacaact aacaactgga
    28381 ttcctttgct ttatttgttt ttgttcagtc taatcttgat aatcagttca tttgttttag
    28441 aggaacatcg agatttgaac accggctata tccccgaacg tgaaggaaaa ccgtatgcta
    28501 aacgttctct tttatccttg cgtggtttat tattctatct aaataaaggg atgacaatca
    28561 gttggcttat cacttttggt ttgatggggg cggcatacgg ctctatctac gggaatatgc
    28621 aacagttttt agaaagtaac gacttaatga aacagatgtt tatccaatct ggcgtctcaa
    28681 ttgaaaaaaa ctttacaagc acgatcatgg tagtcttgat ttgtctagct gctattttac
    28741 caattgctag catcaatcgt ttatttacag aagaaaccca cggacgtatg aatcagctgt
    28801 tcgcaacaaa aatttctagg aaccaacttt attggaccaa tatgttacta gctattctat
    28861 ttgctatatt ggcaatcctg attagttctg gtgcactagg gctttctgct ttggcttcga
    28921 tgaaggatgc gaaagacatg gatctattga tgtttcttaa agccgggttc aatttcctgc
    28981 ctgccgtttt agtgtttatc ggaattagtg gctttctatt cggatggctt cctcgaatcg
    29041 gtaaacttgc ttatatctac ttagcttact cctttgcact caattacttt ggcggaattt
    29101 tggatttacc agaatggttt tccaaaacag ctgtccctag ctgggttcca cggatgccaa
    29161 acgattcttt tgatctttcc gtgttcctta cgttatcatg tatcagcctt gcactgttcg
    29221 tccttggttc tattggttat aggcgaagag acttactaga aggataataa aaaagcatgt
    29281 acaatttcat gctagcctcc aaatctgggt caaaaatcca acatttggag gtcatttttt
    29341 tatttacaag tcttctaact gctctttatc aatgatccag acttctcgat tctttctttt
    29401 tacataacca ttctcctcta ataatttaaa ttttctagat aaagtttcgg gggtcgtacc
    29461 gataaatgca gcaagttctt tcattttcat tggcaggaaa aagtgatcct tccctgtcgc
    29521 tttagctaag tcaagcagat agacagcgac tcgttcttct actttttcca tcatcaaaaa
    29581 agttgtctgc tgctgtactt ttgttaattt ctcaacgttg attgctaaaa gtttcagact
    29641 aagggcggga taatgcagta agagttccga gaaatctttt tgtctcaata tgcaaatttc
    29701 cgttttttct agtgcttcgc caaataaaga ctcatttgga acgcctagca actggctctc
    29761 tccctcatat ccgcccggct ctattactcg taaaagctgt tcctttccgc tagcagataa
    29821 ttgatacact ttcatactgc cggaggccac gatcactaat tgaggttctc cattcggcgc
    29881 aagaatctgt tctccttttt ccacatagtg atgtttgaca agtgcgtgta ctttttcttg
    29941 atcttgctgt actaagtgat tgaacaaagg aacaaatgaa acacataaat gatcatgact
    30001 cataatctct cctccttcta aacagctatt tctaataaag aaacataact tcccttattc
    30061 caactgataa ttttctttta tcatagcata aacgtcaagc gaaccagata ccagcaaata
    30121 gagtggattt ctttttgtcc cttgctttat tttccttctt ttgagacctt aagtaattga
    30181 ttcctatcaa ttttttaaat atttactttt tgaataatca aaaacaaacc ttcactcgca
    30241 agccgagagt aaggtttgtt tttcgcattg cttatttcgt gttgtctatt gaaatttttc
    30301 cgcacctagc ttttgatata gtggtgctag ttcttgtaag gtttcagcgg ttatttgctc
    30361 aacgtgttcg atttcttgcg gatagaagaa tagtttaccc atttgaaagg ttcggtctct
    30421 catcgctttt ttcaactctt cttgtttctt tgtatcggac aaatagtcag acaaagctaa
    30481 gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa taatccttga tatcgactgt
    30541 tttgctccac tctttcagta tcgcaagcaa ctggttatac gctgcaatgc taccatatcg
    30601 ttcttcgctt ttataatgtt ggtacatcaa atagatttgg tattgttttt gattcaacaa
    30661 aactgctaga caggcattct cgtcttgacg agattcgctt cggtacgcgc tccaaaaatg
    30721 actgcgtaaa ttccagccat tcgtccagct ttcgatttta ggtttgctca tgccgtacat
    30781 atcaggcaaa agtgcagctg tctggagctg aatctctttc catttttgcc agtgttcttt
    30841 atattctttc ttgatttgtt caatcttatc tgttggatgg ctctctttga gctgtttgaa
    30901 tgcgtagatt ggtcgatcaa acaactgata atctgatttt ccaagttcca ttgtttcgcc
    30961 tctttccgtt atcttttgta ttctacctga attttgcgat atggacaaat agaggttgtg
    31021 aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca aaaaactgcg atgagcatca
    31081 ccgcagtttt attgaactta aggttgattg atcacatcta aaagcgttga aaataaaatg
    31141 gcactagaag cagctcctgg gtcgatatgt ccgattgcac gctctcctag ataggaagct
    31201 cgtccttttt tagctaccaa gactttggtg tgttcttttg cctggtcaat gatttctttt
    31261 gttaaattcc ctgccttcaa tgcctctgtg actggcagcc aaacatctaa catcgtttta
    31321 tcgtcagctt ctgcctttcc tcggctttga atacccgctg ttccctgctc gatcacttcg
    31381 cctaattttt cttgagaatc aatagaatcg acctcttttg tcgctttcat catgttcatg
    31441 aatgcagatc cgtataacgg accagaagcc ccaccgactt tcgatatcat cgccatagag
    31501 aatactttga atgtatcgct gattgtttca ggatgatcgg cttgaaaagc ttcttcataa
    31561 gctgaaaccc ctcttcccat gttatttcca tggtctccat cgccaatcgg ggtatctaga
    31621 tcactgagat attgtttgtt ctgattgatt gcctctgcaa attgcgtgag ccattcttgg
    31681 atttcttgaa cggttagttt catattatca cccttttatc ttgagatttt ttaccatgaa
    31741 atcgtctgta cattgctttc taaagaatct ttccattgtg tctccgtcaa gtcgatcagt
    31801 gttagagaaa gaccttgcat atctagtgag gtcataaaat taccgacttt tcggaaaact
    31861 acattgacgc ctctttcttc caacagagct aaaacatcat tcatgaacac gaactgttcc
    31921 atcaaaggcg tcccgcccat tccgttgact agtacacctg cttcttttgg taaattcgag
    31981 taatcatcta aaatcttagt aacaagttcc tttgccagta ctttagaagg ctgcatcttt
    32041 tctcgacgat agcctggctc accgtggata ccaacaccat actcgatctc atcttctcct
    32101 agttcgaatc ctggtttccc tacttctggt acagttgctg ctcgaagcgc tagaccgatc
    32161 gtcttagttg cctgtacgac cttttctccc agttctttca actcagctag ggaagcaccg
    32221 tttcttgctg catcgcccac gattttgtgt acaaggacag tacctgcaac tccgcgtttt
    32281 ccagcagtat acgtactatt ttcaacggcg atatcatcat ctacgatcac actttctact
    32341 tcaatatcgt ccatagcagc taattccttt gccatatcga agttcaatgc atcccctgta
    32401 taattcttaa cgatcagcaa aactcctttt ccactatcgg cttccttaat agccgtctga
    32461 atctgatctg gagttggaga agtaaacaca tcaccaagca ccgctgcact taacatccct
    32521 tcccctacaa acccggcatg ggatggttca tgtccgcttc ctccaccaga aaccaaacca
    32581 acttgttccg ctttctgatt cttggctaca acacgggaag attctacacg atgaacaagt
    32641 tctggatagc tttttactaa tccttccagc atttcttcga caatattgct tggatcattc
    32701 atgatttttt tcaatttatt cccatccttt ctttccggca ttttgtgctt ctgttaagat
    32761 ttgctgagta tcatctgtta taccggcagt gatggctgct gcaaaagctc cttccactaa
    32821 aggcgcatcc accagatgat aacgttgctg ctgctcttct tccaacatgt cttttgccag
    32881 ttccgagttc aataccgcgc taccgatatc cgcaaaaaca aaataactag ctgcatcgct
    32941 tgattcattg actgcttcta cgattttcat cgggtctgat ccgatctcgc catttattcc
    33001 gccgagagaa aaaatttgca cctcttcgga tttttgcatc tgctcaatca tttctttaat
    33061 accatctgtt actttttgac tatgagaaat aagcaaaata ctttttttca caagaatctt
    33121 cctttctttt acatagttgc taacgattgg tttcgctttc attaatgaac aattcaagta
    33181 tataataaat ttctaatttt gggaaaatat gtgtagtgct tcttgaagtt tgttatactt
    33241 taattaaaag agaggagtga tcgaatgatt acaacaacaa cgaacagtgt agagggaaaa
    33301 gccgtagtag cttataaagg gatcgttttt ggtgaggtca ttactgggat ccatgcattt
    33361 aaagacttgg aggcggggtt gcgaaatatc tttggcggcc gctcaaaaag ctatgaaaac
    33421 gaactaatgg gtgcaagaga agaagcactt gccgaaatgg ccgaacgtgc aaaagcatta
    33481 ggagctgatg cgatcattgg cgtaaaaatg gactatgaag tattaggcag cgacaatggc
    33541 atgctgatgg tcacatgtag tggaacagct gtctccacga gataagtagg ggacatctgt
    33601 tttaataact aaagagagat gggacatgta gtggcactca agaattagat ttttcagtct
    33661 aacttcgagg tactctacct tcccatcctc tcttttttat ttctttttgc ggatgattcc
    33721 gtaagctgct gcgactaaca cacccgcccc tgctaaggaa taactgacag aagctgtttt
    33781 ttcatttgtt tttggcaaag tctgacttgt tgatttggat ggctctgtcg tttgctgaac
    33841 tgtttctgtc gtgcttccag aggtcactga tttttctcct gccgaatgca aaatatcttt
    33901 tattcgttgg aaactagctg ctaagtcagc tttttttgct tcaaaatcat tagcaactgt
    33961 ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc gctgtgatat ccgcttgagt
    34021 ttgacttagc tttccagaag ctgtttgagc aacctgattt aattgctgat tgaattcttt
    34081 tgcttgttgg atcttcccgt caagatctgt ttggacagct tccgcttttg ctttcgcttc
    34141 ggcaagcttt ttctcaagtt gtgcacggtc atttcccact ttatttttaa gatcagctaa
    34201 atcttgatcg attgctgctt ggtcttcttt caatttgttt tttgcagctt ctaatgcttc
    34261 ttcgagttct tttcttttat tttccaagtc ttgcaaatga tcgatttggt cagaagaggt
    34321 agaactattt attgatgtat cttctgaaga tgaaatcgtt tctcctgaat tatctgtcgg
    34381 tacattggaa gtcatttctg tttccgaggc accgttttca gcaccagaag agcttatggt
    34441 tgttgaaggt tctgtttgag caggttcaga agtttccgca aaagctgtct gtcccaataa
    34501 gaaaatactg gaaacagcaa ttggccagat taacggcttc attctttttt tcatcacttt
    34561 tcccatcctt actacttttt aactaatttc ttattataag caaaaatgtt tggaagaacc
    34621 ataaacacaa caggctgttt gtctaaaaaa cggtgcatct tttatttttt tgtacgttcc
    34681 gggcttttcc atgtcccgct tccataacct aaaatcgttt tgactgctgg aatgaaagtc
    34741 gtcacgatcg ttatcgcgtt cactatccag aacaaaagca tatatagtgg tgcgaaaaga
    34801 aggtatttca attttcttcg tcgatcatct acaatcagcg aagctagcaa ctggaaaaat
    34861 cctgcgacca tttcaaagca gatgaacaca aaactgaatg taaaagtaat ataaatctgt
    34921 tcgtagtttc cttgatatac ataatgcccg attaatccag caaaaagaac tacggaaata
    34981 caaaagaata ttgaccagac aatactcaat gtttgatcaa tgaagatgat cgttcttcca
    35041 atatgtttaa aaggatggag catcactttt ttgaaattcg tcagccagac ttctgttcca
    35101 ccttttgccc aacgttttct gtgtcgatat agcatcttca acgtcacagg tacttccatg
    35161 aaaaagataa tctcaggagc aaaaacagat aaccagtcat ttaactgatg atcccaagca
    35221 atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc ccacatcgat caaggcttct
    35281 tttcgataca tcgtatttgc tccactgtac gcataaagac caccgaaaac agctgactgt
    35341 gttcgtttaa tgatgcccac gatacttgag aattcaaccg tttgagattt tgcaatcaat
    35401 ttggtacgat tttggacatc catatttgca gtgaccgcag caatatgtct tgcaccaggg
    35461 cgaatgaaat aattcacata cctaatcagt gcatctggct ccggtaccgt gtctgcatca
    35521 ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga tattaaacgc gtgggctttt
    35581 cctttatttt tttcgatccg aaccacacga agattagcgt attttttcat cagtctagct
    35641 aatatttctg gtgtttggtc cgttgaacca tcgtctgtca ctaatacctc atagttgtga
    35701 taattcaatt tcgtcatcaa atactcgatt gtatattcga tcacaatttc ttcattatgg
    35761 gctggcacca tgatcgtgat gaacggttcg acactcaagg gaacatctac ccattctttt
    35821 tgcttatgtt tgtataaaaa cacataacac cagacaccaa taaaccatac aaatccgcct
    35881 aaaatcggat aagaaacgag aaaaaaatac ataatagaca aacaagtatc taagaataaa
    35941 ttacctgtat gggtgattga gatcatttca gttccacccc attttcttta tacaaatctg
    36001 caatgaatgt cgtatctaga tttttctctt cggagacaga ataaaaacgg accgtttctc
    36061 tctcttcttt tgttccaaaa cgttccgtat acacttcttc aagtaattct tgtctttttt
    36121 tcaatttttc atcttcatat tcaaaagatt tatttacttt tttgtataga tttcgattgt
    36181 tccacctagt caatacaaat gaggtaatca ggaaactgaa aaaaatcaat acaaaaaagc
    36241 cgattaagaa atacagcatt ttcttttctg ataaaaaagc gtacaaggaa aaatccttgg
    36301 ctctttctgg tctcgttaat gggaaaaaga cccatataaa aggaagaaca acgatcatcc
    36361 atcctaaaag agccactaac gtttgccaaa tctttaacca gtaattacct tttgaaaaat
    36421 acttatcttt caccaaaatt gatgcaactt cttctcttgt tacttttttc atatcctcac
    36481 ctcacgtttt tttgttacga cgtaggagtt taccatacat ttcatttctt ttcacataaa
    36541 gtgttcctaa atttttccta agaaaaagaa caaaacattt ctcatcagca aatgaaaaat
    36601 attttgttct ttttatcagt tgttcttttt attttttatc tcttaattat gttgctcttt
    36661 ccaatacgac aatgcttttc attcctctag atatcgccgt gtacatttgt ctgcgtcttc
    36721 tttgctcatc tccatcgttt tcaaatggat ataagattac atgatcaaat tccagtcctt
    36781 ttgctagact caaaggaatg atttggcagt tttctaatcc atatgaattt tttttaatcg
    36841 attcttgtag cgtttgggtt tcctcttctg tttgggtgat aactgccgct ttttctcctt
    36901 ttagagaaac caaaatctca gctagtattt tcaacaattc ttcagaggat cgaaaagagc
    36961 ggtattcggg ttcttttcct tgcggtcgga tcgatacgat gtcgatcttc tcttgatcca
    37021 cagcataggt tttgaaaagc tctgtgattg ctccgctaga acggtaacta tttctaagat
    37081 cataccgatg gatcggcaga tggtagtcgt cgaaacaacg cataatatca gcaaaagtag
    37141 tggaagaatt aaagattgcc tgattctcgt cacctactaa agtaaagtga gtttttggaa
    37201 ataattcgat caatagacct aattgtgcca gggtataatc ttgtacttca tcaatcaata
    37261 gatagcgaaa tgctggtaca tttacctttt caacaagtaa atgacgaata gctaatataa
    37321 tgactgcttc atcaacagta cgtgtgggtt gccaagcata agctgagcca tatcgttttt
    37381 cataaattct ttcaaacaac tgatgctcat ccacccatgc catttcttcg atttgtctgg
    37441 ttatttttcg atattttttt tgtaaaagtt tccttgcata agctacaata ctttgttccg
    37501 aatcatcaga aatcaattta ccgaataatt ccaactgcct atcttctgtc aacgagagta
    37561 cctgattctg cacagcagga ctttttgctt gtttcaaaag atgattcttc cattcgctca
    37621 tcagtgcctt tttcgttgct tgaagcctat cgatcaattt aggatgagca ggaactttct
    37681 catatatttt ttcgatcgtt tttttagaaa ggactgtttt tccattttta gtaagtccac
    37741 ggataaaatt cggatgatct aaaaacaaag gatctgattg ttttagatta tcgataaact
    37801 ttttgctacg caaacgttct gtctgctccg atacattttc tcccgtgata cgtttgaaat
    37861 aagcctcttc accttctatt tcctctgctg aaagctgtga aaccagctgt atgatggtta
    37921 gattcagtgg atttctttca cctaatgatg gtaggacatt cgaaatatag tcgataaacc
    37981 gatgattagg tgatagaatc agcaagtcat cggatgtcat cgtatctcga tataaataga
    38041 gcagatacgc aatacgctgc atgatagcag aggttttacc acttcctgct acgccattta
    38101 ccaagatatt ttgacttttc acatcccgaa tgattgtgtt ttgttccgat tggatcgtag
    38161 cagtgatagc cttcatttca ttggtctcat cttggcctaa gacttctaat aatacgtcat
    38221 cttgaatagc tacagacgta tcaaaaaact tgaccaaacg atctctttcc aaaagaaatt
    38281 gtcgtctttc tttaataacc gtatcgattt tattttgacg aaccgtataa gaagatggac
    38341 caatcatgtt attgtaaaaa agttcggcaa ttggtgaacg ccaatcataa acaagatttt
    38401 catctgcatc atctgtgaaa tttgctgtac cgatataaaa ggattctttt ggctcattat
    38461 ccagaaagtc tacaacgact tttccaaagt agggactttt caataaacgt tcgatctttg
    38521 ctagctgatt ttctgcgttt ttctgtttga tattcatctg atcgatttct ctatttttca
    38581 tttcaatcat cgaaaaagta tccaggttat ctaagtaact gtcaaaattc aatcgaagct
    38641 cgccagccat ttctttcaac gactggtttc cttctatttt tgccgtatcc atcgattccc
    38701 gcagttcgtt ttttttatca attagctttt gatacacgtt cttcagatat gcttcttcta
    38761 actgtttttg tgtgttgacc atacacattc tcctcttttt ttaaagtggt ggaaccgatt
    38821 agtttacctc ttttaagagt cgaatgcaag ctttttaacg agtatcattt gttaggatat
    38881 atcagttctt caggtcggac agtttgatat aaagaaaatg caccatcaag gttttttacg
    38941 tggtagccag actgcttcaa gattctttcc gctagatagc tacgtaatcc gctatgacag
    39001 ctgacgatat attcttgttg gctgtctaat tcatttaatc ggtcccgaag ttcattcaaa
    39061 gggatatgtt tggcttcctt gaatttgccc ttttccaatt cttctgctgt tcgcacatct
    39121 aataaaattt ttccttttgc tagttctgtc ggcaattcat gccattgtac ggtttcacta
    39181 agcccttctg caatgttcat tgctgcatat cctagcatgt ttactggatc tttggctgag
    39241 ccaaatggcg gtgcatacgt caattctaat tccggtaaat cgaagatcgt caaatgccct
    39301 ttgattgctg ttgctaaaat atcgatccgt ttatccacac cttttgcccc aacaccttgt
    39361 gcaccataaa tctcgccagt ctctggatgg aagatcaact tcagtaaaat atctgttgct
    39421 tctggataat agcttgcatg atcttttcct gatacatgaa tgacttcaaa agacagttcg
    39481 ttttctttgg ctgttctttc acttaaaccg gtggatgcgg cagtcattcc aaacgttcgt
    39541 ataattgctg ttccaatacc gccttgattt cttcgagaaa ttccggaaat cgtgtccgca
    39601 acttgtctac cttgacgatt ggctggtgaa gcaagagaaa taagtgcatc ttttccagtg
    39661 atttgctgtt ttacaacgat agcatccccc accgcataaa tatcaggaat attcgtttga
    39721 taacgttcgt ctactaggat acccccgcgt atgcctaatt cgattccagc ggtcactgct
    39781 agctggttat caggacgaac accaatggat aaaataacta aatcagcagg gatggtacgc
    39841 ccatcatcca gtcgcaatcg tttcccgtgg tcttcaaatc cagcgacagc atgagaagta
    39901 atcacttcta ctccttgatg agacaattct ttttctataa aagctgccat ttcttcatcc
    39961 agtgttggaa gaatatgtgg tgcttgttcg atcaccatga cttctaaacc tcttcttttt
    40021 aggttttctg ccatttccaa tccgatgaat cctgcaccaa cgatcacggc acgctttggc
    40081 tgtttttcta atgcatgcat cactttatcg atatctggta catttctaat agaaaaaaca
    40141 ttgtcggctt ctgttatccc tggaatcgat ggaacaactg gttttgcacc aggagataaa
    40201 accaatgcat cgtactgttc aatgtgtttt tgaccctcat gtacgacttc taccgttttg
    40261 ttttcgggaa agatggctgt cacttcatga tttggacgca catctaaaca aaaacgagcg
    40321 gataatgatt ctggcgtttg gacaagaaga ttttcccgtt cactgatttc tcccgaaaga
    40381 taataaggca agccgcaatt tgcaaaagaa acatacggtc ctttttcaaa aacaacgatt
    40441 tctgcatctt ccattaatcg gcgaagccgt gtagcagcag acattccacc tgctacacct
    40501 ccgacaataa cgattttcat cttatttccc tcctactgtt cgatcaaacc attgattcat
    40561 gccaccacgt acattcacta catcataacc agatttcttt aattctttag ctgcttgttt
    40621 actcctcatc cctgattgac aaatcacata gacggtcttt tctttgtctc catcatatct
    40681 gtttatactt tgtaatggaa catttttagc tccttttata tgcccgcccc ggtattctgc
    40741 aggtgttcta acatcaagta acgctggatt ttccaaaagc ttttccttta attctgggac
    40801 tgttatcgat tttattgatt gaaatagcaa attcattttc tgcacctcat ttacattttt
    40861 ataaatacct catggggtat attaaacagg agctttatta atatgtcaac cagaatgact
    40921 cttcattttt ttcactactt tttatatttg cataaaatac cgataagggg tattatctaa
    40981 ttataaaaat agaggagaat gtgttatgga gattgataat aaaaatatcc tgaaccgttt
    41041 gaaacgtaca gagggacaaa ttcgtggtat ccaaaaaatg atcgaagacg aaaaagaatg
    41101 tatggatgtc atcactcaat taagcgctgt tcgttctagt attgatcgcg taatgggcat
    41161 gatcgttgca gataatctga aaaattgctt tgagaaacct gatagtaatc cagaagaaca
    41221 agcggctaaa ctagaacaag cgatcaagat gattattaaa aagtaaagga atgatttcaa
    41281 tgaaagtcgt tttccatatt gatgaattag aaaaatggtc agaaacaggg aaaaacgtaa
    41341 aaaacctgat aaaagcttct ccagaaacga cgattgtcgt atctgtaaat ggcatcgcga
    41401 ttaccggtta tctagactct gccaatgcag aattcttaga tttgcaagga gtcgtttttc
    41461 acgcttgcgc caatgctatg cgtgctaatc atatttcaga aagttcttta cctgaacaag
    41521 taatcgttgt accagcaggt gtccttgatt tagttgaact gcaatcccaa ggttatgctt
    41581 atatcaagcc ttagtcagta gaggttgcga cagaagtgat cagcttcaag aaatttcggc
    41641 ttatttttcg aagaagctac ttctggaaaa ccgtgaatca aaggttgtga aaacgctgtc
    41701 ctgcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt cgtttggttg
    41761 ggcttattac cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag
    41821 cggtttgacc taagtattag agaaaaaaac gggaaaatag ctcctcatat tttgccgttt
    41881 tttgagctaa taccgaaggt cagctgattg aacaccgttt aaggaagaga ttgtgaaaaa
    41941 gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat ggatttctac atttttgcat
    42001 aaatttggct taataccgag aaactggctt ttgaataccg tttaaggaag aggttgtaaa
    42061 atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagctgct catattttgc
    42121 cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaccaa gaggttgtga
    42181 gcctaccatt taactccgaa tcacaagaaa taatttcaaa aacaacttct cctgtttttg
    42241 gttcggttgg cttattatcg agaagtagct actgggagtc tatttatcaa aaaaaggaat
    42301 gcgacatagg tcacacccct tttttatttt ctttgaaaca tctttcggta ctcgacagca
    42361 tacaagacaa aagattgtaa aagaccgtaa gtgatgacgg caaaaatgac tctcacccac
    42421 agcgttggta attctgggaa aagcagcaaa aggatcatgc caacatagat gacagcagtc
    42481 gctactttgc cataccactt cgctccactt aatttgatat ttttagatag cccgattaat
    42541 ccagctacaa acataaagcc ttcttttatc aagaatagac caaacaacag ccaaagccac
    42601 ggtcgatacc atgccataga caaaatcaga aaaagttgtg tcaatttatc tgcaaacgga
    42661 tcaagcacct tccctaagtc tgttacctga ttatatctcc gagcaatgac cccgtctaga
    42721 taatcagaca aacctgagac aacaatgacc gctcctgcta tataaaatga ctgtctcagc
    42781 acaaaataaa gataaatcgg cagcaaaaaa atacggaata tcgataaaag attcggaatc
    42841 gtttgtattt ctttacgcca gtctgtgctt aatttcattt tatcgcccta cctcctcagc
    42901 actcttttct ttttatttta gcttacacgg ggaataaaac caatgatttc actgttttta
    42961 tttttcttta cagacactga tattatgagc taatttgaga taatcacttg ctgttttcat
    43021 cgtttactct tctttcttcc tgtcgtttgc gcatttcttt tactagtact ttatgagctt
    43081 tttttgatcc tcgctctagg tcacgctgcc gtttacgtgt tttataatcg ataaacagac
    43141 tgtctaaatc gtaacctgtc gggtacagat cttctgctga acgcaccaga cgaacacgct
    43201 gacgaggcac gtggattatt tttttgtcta aatatacatc aatcgtatct tcacctatat
    43261 cttcataaac taacgcttct ttttggtatt cattgacaaa tatccgatca ccttttgcaa
    43321 agagctgacg ttctttttta ttctccttcc tatttgtaac tgctgtctta ggaagaaatt
    43381 cttttttctg tgtccgatag gctttttccg ccaagtaacg ctctgcttga tggatcaatt
    43441 tcatcgacat atccatttta tgagcaatcc acaaagcttg gctttctcct gtttcttcca
    43501 acccgcaact gatatttagg acgaagtgct tcccgatcga aagccatagc agccggcaca
    43561 aaatcttcat gatctccggc gaatttcttg atttctccat aatgggtcgt ggcaatgatc
    43621 aatgcccctt gctcatacat agattccatg attgcgatcg ctagtgcagc cccttcattt
    43681 ggctctgttc cactgccaat ctcatctaaa agtaccaacg tattcctttt tatctttttc
    43741 aaaatagcag cgatgttttg catatgacca gaaaatgtac tcaacgcatt ttctaaattt
    43801 tggtggtcac cgacatcgac gaaaatctca tcaaagacag caagctctgt cccttctttt
    43861 gccggaactt gcataccgaa catcgccatc aaagtcaata aaccgactgt cttcaacacg
    43921 actgttttgc ctccggcatt tgctccggtg ataatcaagc ctcgatagtc tttaccgatt
    43981 tcaacatcca atggtaccgc atgctcaggt aaaaatggat gtctgccttg cttgataatg
    44041 atatgttccg atttattgat tctaggtgtg cttccattga tttctcggct aaattttgcc
    44101 cgagcaaaga tgatatctaa caccgtgatc gtttcgatca ataaatcgat cacttcttcg
    44161 ttttctgcga tagcaccagt caatgccgct aggacttgat attcttcact gatttcttca
    44221 gcttttaaca actgatagtg ttcattcaat ttagaaatca cagttggttc aataaaaacc
    44281 gtcgttcttt tatttgattc atcgataatc gttccatcta ttttattttt atagctggct
    44341 ttgataggaa tcgtatagta ttcgcctttt tgaacaatca ttgcttcttg gatcatttct
    44401 ttgtttttcg gatgacgcaa gaatttcagt aattttgact ggatttcttt ttcgattagt
    44461 tgtaattgtt tgcgtacttt acgcaaatta cgtgacgcgt cgtcactgac tttattattt
    44521 tttattttct ggtcaatcaa ctcttctaca tttatcaaat caggaagatg tttgctataa
    44581 gcaaacaaca agggtgtctg gtattgattc ttatcaaaaa actttgtgat catccgactg
    44641 cttctaagaa aatccgcata ttcgatcaaa tcggttggct gcagaatcaa cccttttttg
    44701 acttgttctg tcagtgcatc gatcctcggc aaccccataa atggaacatg ctgactgctt
    44761 tctaagatca agcgtgcttc tcttgtttct tcttgacgat ccaagacagt ttgtaggttt
    44821 gtcgagaccg tcatatcgct gattcgtttt ttgctatagt ttccaattgc tcttgcttgg
    44881 atttcctttt tgatccgatc aaattgtaat tgactaatca tttcgttgtc catattctct
    44941 ccttttcttg aagagatacc tgtcccaaaa taaataaggg cctagagata aataaaagga
    45001 tagaacacag cttaaaaaag tcatgtccta tccttattag aatacaaaga tattgttttc
    45061 ctgacaggta ccgtttcata agcacaccaa ataaactttg tttattggct attcgactta
    45121 ctgattagat accagctaca ctcacaaaac ataccccatt cttctaggtc ttatttattt
    45181 tccacttaac tatagcaaga cacattttgg ttgtcaaact ttagtatgtt ttatagattt
    45241 acaccattaa tgaatagaca cctaccgcga taattgctcc gatcattgga cctgcgattg
    45301 gtacccatga atatgcccaa tctgaagttc ctttattagc tattggcaat accgcatgtg
    45361 ctaatcgagg accaaagtcc cgagcagggt tgattgcata tcctgttggc cctcctaatg
    45421 aaagaccgat ggctaagatc aatccaccta cagcgaatac gttcgttcca ggtgcaaatt
    45481 cgttttgtcc aaaagctagc aacccaagaa ctaatacaaa tgtaccaatt aattctgtga
    45541 tcacatttgc tggataattc cgtatagcag gacctgttgc aaacgttcct aaaatcgcgc
    45601 tttcatcttt cgttgcattc caatgtggca aataagaaag ccaaacaaga atggcccctg
    45661 caaaagctcc taaaacttgc gcaatgatga aaggaacgac aagattccaa ctaattgccc
    45721 ctgtcatcgc catagctaga gagactgcag gattcaaatg agctgggccc atgaatccag
    45781 aaatatagac agccattgtt acagcgagtc cccaaccaaa tgcgatgaca atccagccag
    45841 aagcctgtgc tttacttttg tttaaattca cagccgcaca cacaccatct ccaagcaaaa
    45901 ctaggatcag tgtcccaata aactccccca tgatttgagt catatcactg ttcatttatt
    45961 tctccccctt tagttcccgc agatctgatt ctgcaatcaa tgcttctaaa cgtttagtct
    46021 gttgcgcctt ctcctcttct gtccattcaa aatatccagc aattgcatcg atcacaggtt
    46081 gcttgatctc atcaagctga tctcgttcaa acaacagatg atttgtacga cgaatgagat
    46141 aatcgcctgg ggccaaaacc atttcttctt ctaaaccata acgtaaccga gctgactcag
    46201 cgagactcaa accaggataa ggtgccattt cttttgctaa ttcaaagata cgtcgggcat
    46261 tagtcccgta aaaatcagcg atataagtag catcttcttc ctctaaaccg gctgctactc
    46321 cttcttttgc taattctgtc actgtttctt ctaatttagt tggatcgaag tttccacctg
    46381 aaatctgata ttttttagag tcgatttctt tcgtctctat tccgtattct tcttttaata
    46441 attggcgtat caatcgcaaa gctccttctg ccatcttacg gtaatcggtg attttcccac
    46501 cagataaagt cagcaaacca tctggttctc tttctaaaga actacctctg gaaatcgtag
    46561 aaggtgcttt ttcatcacgg ctgttttcca aatggttcaa cacgtcttct acttcagcac
    46621 gagaaacttt gttttcctta tattcactta ctgtatcgac aactttattg aagctcttgt
    46681 ctgaaatcga tccattatct ccaccattgt aatcagaacc agaattacca atcaacagtg
    46741 gacgaagtcc tgcccagctt gcttcgatat cattcaatgt gatatttgct tctggatagc
    46801 gatgattgat cacatccaat agataatcta catcttcttg tgtgactttc ggatccgtaa
    46861 aatctccttg gtaatccgta tctgtcgtac caaagtaagt cttgttttct cttggaatag
    46921 caaaaaccat ccgcccatct tgctttcctg tatcgaagta tgtgggttgc ggtacaggca
    46981 gttttttcgc atctacaact aaatggatcc ctttagttgg acgcatttta ggagagactg
    47041 gacgcgtaaa attcaagttt cgtactttat ctacccaagg accactcgta ttgatcacta
    47101 attttgcttt gatttcgatc acttcatctg tcagcaggtc acgagctttg acgccaacga
    47161 tttgatcgcc atcatataaa aagccaaccg ctttcatttt acttactaga taagccccat
    47221 cttcggcagc ctttttgata ttatcaatca ctaaacgggc atcattgttg cggaaatcta
    47281 gatagacacc cgcgcctttt agcccttctt ttttcaaaaa cggttctctt tccaatactt
    47341 cttctggtgt cagcgtataa ttctcatatt tggttcctgt cacatttgcc agtttgtcat
    47401 aaaggtccat tgctactttg accgagaaca tattgaaggt tgttgctcct tcgccctcat
    47461 agattggcaa aagcattgga tctggttttg ggatgtgtgg ggcaatactt tgtacgactg
    47521 cacgttcacc aactgtgtca gctactactt ccacatcaaa tgtcttcaga taacgaatac
    47581 cgccatgaac aagtttggtc gagcgagagg aagtcccttc tgcaaaatcc tgcatttcga
    47641 tcaatcctgt tttgattcct gatgctgctg actggactgc cacaccggca ccagtgattc
    47701 cgccaccgat gatcaacaga tccaattctt cttgctgcat tttttgaatg ctatcttgtc
    47761 ttgtcttgtt tgaaaacata tggatttcct ccttattcaa atgcttgggt agcttttact
    47821 gcttttttcc aaccgttgta aagtttttct cggcgctctt cctccatttg aacttcaaat
    47881 agcttgccag cttcataaaa ttcgcggatc tcgtctgtat ctttccaata accgacagct
    47941 aatcctgcta aaaatgctgc ccctagcgcc gttgtttcca agttttctgc tcgttggatc
    48001 ggtacattca agatatctgt ttggaattgc atcagatatt cattgtttgc tgcaccgcca
    48061 tctactttca atacaggaat cttgattcca gtgtcatctt gcatcgtatc gatgatatct
    48121 ctcacttgat aagcaatcga ctgtaaagta gctttaatga tatcttcttt tgtcgtacca
    48181 cgtgtcaagc caaacatagc ccctcgtgct gcctgatccc aataaggagc acctagccca
    48241 acgaatgcag gaacaacata aacctcatct tcgcttgtag agcgtttcgc tgcgtcttct
    48301 gaatcgctag ctttttgcaa catttgaagt ccatctctca gccattgaat cgaagaacca
    48361 gccacaaaaa tacttccttc taaagcatag taaacctttc catttatgcc gtaaccaatt
    48421 gtcgtcaata aattattttt ggataattgc ggtttttcac cggtattcat gacaataaat
    48481 gaacctgtac cataagtatt cttgaccatg cctggttcga atgccatttg tccgaataat
    48541 gcggcttgct ggtctcctgc catccctgaa attggtgctt cgcttccata aaaatgataa
    48601 ccttgcgtat aaccataaac ttctgagtta gaagtgactt ttggtaacat cactcgagga
    48661 atatttagta aatctaaaat ctcctgatcc caatctagct gatggatatt gaacaacatc
    48721 gtccgactgg cattcgagta atcagtaaca tgtgcttgtc catcggtcag tttccatact
    48781 aaccaagaat cgatcgttcc aaacatcaat tcgccttttt cagctcgttc ttgcgcgcct
    48841 tctacatgat caagaatcca gcgtatcttc gttgctgaga aataggcatc gatcactaac
    48901 cctgtttttt cgtggaaaaa ttcttgatag ccttcatttt ttaagttatc tgcaattcca
    48961 gctgactgtc tggattgcca aacaactgca tgatagattg gacgtcctgt atctttttcc
    49021 catattactg ttgtttcacg ctggttcgtt atgccaatcc cagcaatctg atttggtttg
    49081 atccctgatt cgatgaacgc tccggcaata actgactgta cagaattcca gatttcattt
    49141 gcatcatgtt ccacccaact ttcttgcggg aaatattgcg tgaactcttt ttgagaactg
    49201 ccgatgtgtc ttcctttctt gtcaaagata attgctcgtg agctagttgt accttggtca
    49261 atcgccatga tatacgttga ttctgtcatt gtgattccct ccattatatg caaacgcttt
    49321 ctttaactac tcttatcata ctactgtttt aaaaaaagaa actgtcaaaa ttttattctt
    49381 tttttctttt tttgacagtt ttttattatg aagctatttt ttaatcaatt cttttctttt
    49441 ttcagcgtct ctattcttct tttcaatcga ataatgtcgt aaggtgacgc aaattctgac
    49501 agataatatt cacactgttt tttgtttctc tggcaaaatg tgatcaccaa atcatattct
    49561 ttaccttctt cataacttcc aatctcaata ccgccgattc ccctcagttc tctgatcaga
    49621 tattgttgaa aagcaatacg ataaatcggc agactttcta aatcgattcc aatcactact
    49681 ggttttgcaa tataaaaatc gatcatcaaa agaacattgg catactggat catcaggtaa
    49741 ctaagctcac tatcttcagc tcttttcctc ggcaattgct gcaccgctgt ttcctgtaat
    49801 ttcgccagta actgtgttaa ggaatgcccg agcattttct gctgctgttc caaaagccga
    49861 tcacgctcat agacttcgat ctttccaaca aagaaaaacc attcattgtt gatctgggaa
    49921 atttggtagc ctactgcctt ttcttctttg attttcaaac gacgaggacg ataatgccat
    49981 aacatcgttt cccgaatgta agtatctaaa acagcggttg gtaacttttt gctccgtgtc
    50041 aaatcatact gataaaatat ttcttcgtct actatcgaaa aagaaacaaa aaagctgtaa
    50101 aacatcattg tttcgtatac attcagttct gctgctgttc gactaagatg catcacgatg
    50161 attggatcga ttgcttgata aagccggtcc gactgataga gtaatttctt ttctttcaaa
    50221 gttgcgtacg tacttttttc attcaatagt ctttttttcg taatcccgag ccagcaagca
    50281 atctgtgctt ctgctgatgc cgtaaacgtt gtttctaata cacggtccaa tcctagaatg
    50341 aacggtcttt tttctggtgt attttgcaaa aaaagcggtc gctgatcttc cgggatatat
    50401 tggaatagtt cataataaaa atatcgtatc tgtagctctt ctccgtataa ttgaccgttt
    50461 ttgatttgta atccaaattc ctctaagagc tggttcaatt ctttgatctt tcgaaaaaca
    50521 gaagactcac tgatattaaa cgctgtcgct aatcgaacga tggatacttg ctgatgttct
    50581 aataaataga cgagcatctt aaacttcaag ctttcctgca ataaaaacaa tagaattttt
    50641 tccaaactta catcttcgtc caacacaagc agcgcctgat actcatttcg ctgtacttct
    50701 actttttttt ccatcattcg gccaatcaat tcaatatctt ccaaatattg atcaaacgag
    50761 gcttttgaca gttggacatg ctctctcaaa ccattgatag aggctgttcc cccttgcaga
    50821 acaaggtttc gcaaaatcag gatctgtaac tgctcttttc tttctaataa tgcttcaatc
    50881 ttcataggca gtattcgcca cctttgacca caccatatct ggcacacggt attctggctg
    50941 gaaaaatagc tcatgttctt ctaactgttc aaacgtccat ccttcttcta ctgcaattgt
    51001 cagttggtca agaaggtgga acatctcttt tttgctttgg agctgtgctc caagaatctt
    51061 tttttcagaa tcaaatagaa atttgagttt aactttgctt tgatttattg ctgtaagctt
    51121 gtagtctacg tatcctgttt caattttttg gggataaaaa atgctctctt cttctgtcaa
    51181 acctacgctt cctaagaaat agccaaacca ctgattgccg actggacgat aggttccctg
    51241 atcctttgtc tgtgagccac taattgtctt gctgacattt cgtgctgtac ggacagcgtt
    51301 atttactaga gaaacataca ttttttcatt tgttggacga aaagttactt ggatcgcatc
    51361 gccaatcgca taaacgtctt tttgagacgt ttgcaagtag tcatctaccc agatcgttcc
    51421 atcatcattc aatgttaatt gttcttccca catctgattg tcgggacgag aatgattagc
    51481 cagcaaaaca agatcatagt tctcctttct tttttccgtc tctacaatag cggtttcatt
    51541 ttcggtcaaa gacacaactt gttcttctaa aaaaagtgaa agcccttgat ttttcatttc
    51601 ctttactaac ggttctgtca tttcaggatc tagataacga aaaagaattg actttcggct
    51661 ttcgtaaaga tggatttgtt tttttcttga tgccaaccct tcagctattt cgattcctgc
    51721 ttgtcctgca ccaataattg caattttctt tgcattcatg atcttttgct gaagagccgt
    51781 tacgttttgt atctttttaa ctgggtgaat aagcgttgat tctgcagaaa tatttcgaaa
    51841 cttttgattt gaaccagtcg ctagaatcaa acgatcaaaa tccaattttt ttcctcctgc
    51901 taaaacgact tttttttctt tcaatcctat tactgtcttt tctgtgtaaa catggattcg
    51961 atagcggtca gtcagttctt ctttcgtgat ccaagaaagt tgtttttcac tggtaacctt
    52021 tccttttaat atcaaattga acccgctggg aatccatcct aacttcgatt gcttttcaat
    52081 caaatatatt tcacttgttg gcatcaattg gcgaagataa atagccgcat gaatccctgc
    52141 aaacgacccg ccgacaatta caatgttcat ctgcattcct ctttcttata aggtgttcta
    52201 ctgctaactt ctcaatcaga aaacacagta ttcataactc gctttcaacc ttatgataca
    52261 tagcgatgca gcagaatcaa ccctctccat cataccgtgt tctaggtctg cttctcaata
    52321 atgtactagt ggacctaatt gaagagtatc atttctgaag agatgtttca aaaaatgtac
    52381 tcttataaaa gaaaaaaact cgccttttac gtacctctgt ccaaatagga aacagaaaag
    52441 aacgaaaaga cgagttttag cgtgtgtaat gtaatagtta gaatagacac atcatttatt
    52501 tttaattctt taatgcttca acaactgctt cgcctaattg atgagcagat tgaggatttt
    52561 gccctgtaag aagccgccca tcaatttgaa ccacttctga aaaagctgct ccagactgat
    52621 agtttgctcc tttttctttc aaagcatctt ctgctaagaa tggtacttca ttcgttgtac
    52681 catttgcttc ctcttcttca ttactgaacc cagtcactgt acgtccagct aaaatagatt
    52741 ttccagactc atctgtaact ggcaacaatc ctacaacacc gtgacaaaca gctgagacca
    52801 gcccttcatt tcgatagatt tgtgaagcga ctttcccaag atctattgct tctggaaaat
    52861 cccacattgt tccgtggcca ccagcataat aaaccgctgc ataatcattt gcatccacat
    52921 catcaggacg taaggtattt cccaaggccc gatttctgaa atcttcatct tcataatagg
    52981 cccaatcgat ttcatccata ttcaaaacac tttgcggttc taaaggtaca tatccgcctt
    53041 taggactgac aaaatctgca tcgatcccat tttttactaa tacatcataa aaatgagtca
    53101 gttcacttaa ccacaaacct gtaggacgct cgatcttttc gtacttttct acatttgtta
    53161 atacgatcaa tactttcatt ttcccatcct ctttctataa aaattgcgtt attgataacg
    53221 tgctttcaag aaattatata atgggcttgg caaataaagc tctttatatg cttcttcaaa
    53281 aggtttcgtt acaaattcat cctcacggtt ttccaataat ttctctgccc agtcaggatc
    53341 gacgatcatt tgctgtccaa ttgctgcaag ttctgcatat ttcaatacct gctctacatc
    53401 ttcgcgattg cggatcccac cgacaccaac taaagggatc tttccttgaa tcgtgttatg
    53461 gaccacttca agaatcgatt tatcgttgta tttatcagaa cgagctattc gatcatacat
    53521 atttaatgaa acatgcagat aatctagttt tgactcacgt aatcgttcaa gaagccagat
    53581 cgtgtcttca aaacggatac ctggtgtttc atattcttct ggtgagaaac gataccctac
    53641 gatgaatggt tttttagcat attgttcaac cgctgagaaa accgttgtta ataattcttc
    53701 aataaaacga taacgatttt ccaagctacc gccccattga tcctctcgtc tattcgaatg
    53761 cggagagaaa aattgttgga tcaaatacgt atttgctcca tgcaactcga ttccatcaaa
    53821 tcctgattca atcgctctac gtgtggcttt gccaaatgct tggattgttt caacgatttc
    53881 ttcttcactc attgctcgtg gtgtttccgc atcaggacgt tttgctgcaa ctgcacttgc
    53941 agatacaggc tgttctccag ataaagttgc tcgacttgtc attcgtccac cgtggaaaat
    54001 ctgaataatc gcttttgcgc cttctccttg gattgctttc gctagctcac ttaatcgagg
    54061 aatcatttca tcatgagcga tgctgagttc acctggccag ccttttccga tatcttggac
    54121 atttgcagca ccagtaataa cagcaccaag acctttagaa cgttgtttat aataagtgat
    54181 ctcatcttcg gtgatcactc cattgaaaaa gctgagttgt gtcgtcatag gcgacagcat
    54241 caatcggttc ttcaattgaa ctcctgaagg taaagtaatt gattgatcaa attgtgacat
    54301 tgtcattcct cccataattc gctctgatta acctatcgag aatcatctat cattaaacca
    54361 ctcgattagt tgtacatgca actaatttgc ttaaaaaatt ttattttttt tgtaacttat
    54421 ctgttgcatc attcattgtc tgggctaatt ctttacttgc ttcatttccc aaaactgctt
    54481 tgtatgactg atgaagttct tcccagctac catcgacttc cttagcaaat tggcgtcctt
    54541 gttctgtaag ataaactaat gacaagcgac cttcgctaac tgttttgacg tatcctttat
    54601 attctaattt ttcaacaaaa cgagcaatcg ttgatggggc ggaatagagc atttgtgcaa
    54661 tatctttttg tggtattcca tctttttctt ttaatatcaa taaaataaaa ccataagttg
    54721 gtgccagacc tgtccttcta aacgcttcat cagctaattt attcaacatg cgatctagct
    54781 ttttcacgct aaagaaaaga cattcatcta acgctttttc aattggcttt tccatttcat
    54841 cccctccatt ggcgtacata tcatagtatg atttttattt tttgtcaaaa aacacggaat
    54901 aatgtccgcc tcctctgtct agatttgaaa aaaagaagta gccatcttca aagaatttcg
    54961 gcttgttttc gacaaaactc cttctggaac accgtgaatc agaggttgtg aaaaagtcgt
    55021 ttagttttga gtatgaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
    55081 tggcttaata ccgaaaaact ggcttttgaa caccgtttat atcagaggtt gtgaaaaagc
    55141 cgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat tttgtcgttt
    55201 tttgagctaa tgccgatggt cagctgattg tacaccgttt attgagaggt cgtgagcctg
    55261 ccgttgaact ccgagtcaca aggagcaatt tcaaaaaaca gcttctcatg ttttatgact
    55321 atggtgactt gtgatcgagg agttggcagg agaatactgt ttattcgtaa agaaggggct
    55381 gtgacaagac ttttgtcaca gtcccttatt tccttttatt ataggatttc gccatttgat
    55441 tggatgactt gtctgtacca atcaaatgat tttttcttta accgtttctt tgtgccattc
    55501 ccttcgtcat cttgatcaac atagatatat ccataacgtt ttgacatttg tgaagtagaa
    55561 gcgctgacga tatcaatgca gccccacgac gtataaccaa ataaatctac tccatctttg
    55621 atagcttcct tcatctgctc aatatgctgc ttcaaatagt caatgcggta atcgtcttga
    55681 acggttccat cttcttctac tttgtctata gctccgagtc cgttttcagc tatgaacagt
    55741 ggtttttgat acctatcata taattgattc aaagcaattc tcaaaccctt aggatcaatc
    55801 tgccagcccc attcacttga tggaagatac ggatttttaa ttcctccagt tattaagttt
    55861 ccttggaccc cttcccaatt ttctggatga gcactgactg cactagacat gtagtagcta
    55921 aaagaaacaa aatcaacagg gtaggctgct aagatttctt catcacctac ttctattttt
    55981 aagttgacac ctttttcttc aaacattcgt tgcgtatacg ctggataata tcctcttgct
    56041 tgaacatcac taaagaacaa agaagttctt tgctgctcaa ttgttttttg aatatcatcc
    56101 ggatcacaag aatacggaac aagaagctga tagctgatca tacaaccgac ttgtgaacct
    56161 ttgataattt catgacaagc ttttgtagct aaagcacttg ctataaactg atggtgtgca
    56221 ccttggtatt tcgcttgttc caaatgggga tggttttctt caattactcc tacgctaaca
    56281 aaagggtgat gttttacaca gttgatctca ttgaaagtca tccaatattt tactttattt
    56341 ttatagcgtt tgaatactgt ttcagcgaaa cgaacgtaaa actgaataag ctttcgatta
    56401 ggccatccac catattctgt aaccaaatat aacggcattt cataatggga aagagaaacg
    56461 actggctcaa tcccatattt tagacattcg tcaaacactt gatcataaaa aaataatccc
    56521 gcttcattgg gtgtttcttc atcaccatga ggaaaaattc ttgtccatgc gatagacatc
    56581 cgatagcatt taaaacccat ttcagcaaat aaacggatgt cttctttaaa gtgatgatag
    56641 aaatcatttc cgtgtctttt aggataataa tacttgctgt tgggatcctg cgccgctttt
    56701 acttgctgat gcgtcatcat atgccattga tctgtccatt tttctttagg taaatcgctg
    56761 tcgaatgtat acacatcaga cacagataat cctttttcat ctacatccca tgcaccttca
    56821 cattgattgg ccgctgtcgc tccgccccat aaaaatcctt ctggaaattt tttcatatcg
    56881 atccccctaa acttgctagt tttcttgttt gattattttt cttatgacta gtaatgatat
    56941 ttttgaatct actactattt gtaacaataa ccattgtggt cggatcatat ccctctttga
    57001 ttaattcttg caaatctact tccatcaata aatcgccttt acgtactttt tttccatcag
    57061 atactgcact ttgaaagtat ttgccattta aattcactgt atcaataccg atatggatca
    57121 gtatctctgc tccttcttca ctgactaccc caattgcatg tttcgttgga aaaactgttg
    57181 aaacttcccc gtttacagaa ctgtagattt ttccgtccgt tggaatgatc gcaaaccctt
    57241 ctcctaaaga tccactagca aatgttggat cgtttacttc agataaatca attatttcac
    57301 ctgctgcagg agtacttaac actatatcct ttgaggattc ttcttcgatt tcatccttgt
    57361 agccgaaaag aaaagttagc atacaggaac tgatcatcgc taagaaataa ctagcaacta
    57421 gccaaacaaa tccttcacca atataaatcg ggaaagacaa aatacttctt gtagcaactg
    57481 ccaaagcacc ggctcctgcg gcaccagcta ctccaccaaa tatagctcca catatacaag
    57541 caatgtaaaa tggtcgttta tatttaagag tcacaccata aacagcaggt tcagtgattc
    57601 caaataatgc tgtgataaat gcagataaag ataaactttt tactttctta tttttcgttt
    57661 tcaaaaatac cccaagtact gctcctgctt gcgcagctac ggcagtcata catgctggac
    57721 ctagcgtatc acgaccgtag aaagaaagat tgttggtcat tactggtaca agtccccaat
    57781 gtaacccaaa aatcactaac acttgccaaa atccgccgat aacagcacca gataaaacag
    57841 ggctaaagtt ataaacagct aaatatccag ctgcaatcag ctgacttgca taatctgcaa
    57901 ttggacctac aactaataaa ataattggga ccatcacgat gatacaaatg aatggtgtca
    57961 ataaatttcg aatactttcg tgcaagcttt tgcgaatctt aggctcaata taagaaagaa
    58021 cccatacagc taaaattgct ggaataaccg tacttgtata ccttgctaga atcacgggca
    58081 tccctagaaa tcgcaaggtt atgctatcac tatatgcact gacaatcgta ggataaacga
    58141 gagctgctgc aataaccata gcgatgaaac gatctgtatt gaactttttt gcagcagtat
    58201 atgccaaaaa tataggcaag aaatagaaaa atgcatcagc tgctgcattc aaaatgatat
    58261 aagttcctga ttcagtagtt aataaattca aactcgtaca taaggcgagt actcctttga
    58321 tcaaaccact tcctgccaag acgcctaaag tcggggcaaa aacagcggcg atcatgtcta
    58381 taaaagagtt taaaatgcct tttttctcag ttcctctctt tccactagat gcatcatctt
    58441 ctagtttcat ttgtttggat aatacgtcat acacttcatt tactgcattt cctactacga
    58501 cttgaaacat tcctccagct tcgactacag tgatcacacc ttctagttgc tctaccttct
    58561 ccttatctgc cttcttatta tcttttaggg taaagcgtaa ccttgttgca caatggtaaa
    58621 cattgctgac attttcttcg cctccgacaa atcggacaat atctttcgct aattgtgaat
    58681 agtccatttt tacttccccc ttgttaatta atgccgtagc aatccttctt acaagataag
    58741 tatagttctt agaaaacggt ttctccattc aattagtacc ccttctaata gcgaacgaaa
    58801 ctgtacaata gtgtgtatgg tttacttagc cgagcaaatc actcactgct tgaaagaaaa
    58861 caagtatatc gtttctctta tagtcaaagg agacaaaaaa aacacgcttg atcttccttt
    58921 gctgatgtat aagtggtatc tcaagctgat actctgtgat ttttgtttct acttggcttt
    58981 tgataagaca gcacaacaaa gtctcggcat ttaacggaag cataactagg tcagaactag
    59041 gagagattag ttgctgaatg gaatctatct ttttatggat ttttatttga ttttcttctt
    59101 tgtagtaatg atcaagcagt tttgttttct ttttttcttc aactatggtc agcttttcac
    59161 gcaaaatctg ataaatctcc caagattcaa actcatctag aacactaggt agataaatca
    59221 aatcatccca ctcaaaaata agctcttgct cagtagtcaa aattgcatca tattccgtta
    59281 ttttgtttga ttgatttttg aaaagataca ctggttctat ccggcattct ttgatccaca
    59341 tgttcatact ttgttgaatc tgttttttta gtgtattcaa aatactaatt ggctggttac
    59401 taacaatcag tatctcccct tgttttcgaa aaggattcaa cgcttcggca agatacaaaa
    59461 caaaataagc gctttcagct aaattgggac gtaattgaaa atgtattggg aagaaacgcc
    59521 gaattaaata aatttctaaa ggataacggg agatgatctt ttgtacaaaa tgattcatgg
    59581 tattatgtcc ggcatccatc ctcgttttca tttgtttgat atgggttagc aaaaattctg
    59641 tttcatggaa caataacgaa acaggaagat ctaaaaaacg aataatcgtt tgctcaaatg
    59701 ctcttagatt cttctctatt tctttgtttt tttcatcatt aatcatcaaa taatttaact
    59761 ctaatagtcg attttcaatt gcttgttttt cttgattgga aaaagaatag ggtagttctg
    59821 atgataactt ttttgtgagg gtctctacca tcggaataaa taatgtatac ctgtttgttt
    59881 tttctaaaag ttcttctctt cgaagaatgg tcaaaacgag ataccgagaa aatcgtatga
    59941 agtcctctcc tgaaacaata taattatatg atataagaac ctcttttaat gtcttttgga
    60001 cagtcggcat gatattggaa aaaattgtac tgatttgttg aggaaacctt gcgtgttgga
    60061 tcaccgtttc tgttccaaca agagataaga aaattctttt tgaattttct tcagcaatga
    60121 tttttagtcc ttttgtcgac gaaacttcca gttctaattt aggattgcgc tcgatccaac
    60181 gcaaaatcgt ttttatttgt aatgatacgg ctgtttttga aagaaagaaa atgtctgcta
    60241 tcttttgcat actgacatac cctttttgaa acaataagta cgtcacaata gttgaagctc
    60301 ggaaatccat gctggaatag attttatgtt tcgtcacctc ttgtatgatt tgctgtttag
    60361 tttcagattc aattgattct aaaaaataac ctgaacgttt atttgactgg ataatgattt
    60421 tcttctcgta gatagcaagc atatttttga tttcagtttg gatcgttttc ttgttggtac
    60481 ctaaagcttc agctaattct tcgcttgtta tccaatgatt cttatttctt aatactacaa
    60541 gaatttggag ttgtcgttta tttaagtcca tttgcttaca cctcctcttt gttataaaca
    60601 tacaggaaaa acctctcaaa tgacaatagt cacttgcaga ggtttaaaaa tcatttgaag
    60661 aaagatctat attcaaggtt tttaccatta gactggataa tttgtttata atattcaaaa
    60721 ctatcttttt tcaatctcct aaagcttccg ctcccatcat tttgtctatc cacataaata
    60781 aagccatatc gcttcctcat ttcccctgtt cctgccgata cgatatcgat tggtccccac
    60841 cataagtaac caatgatttg gcaaccatct tttattgcct cagcaatttc ctgaagatgt
    60901 atttttagat actcacaacg ttttggatcg tggattcgat tcaattcatc tggtgcttca
    60961 tctagtccaa tgccattttc aactatgaat aatggtaatt cataacggtc tgtcaattca
    61021 ttcatgacat aacgaaaccc tagaggatca attggccaac tccaaggttc gggagatttt
    61081 tccttcaaaa agggattatc gaaaccattt gctccgccag tatctacgcg catttctgct
    61141 tcttttttat agacagcgct acgataataa ctaaatgcaa tataatctgc cgtgttagct
    61201 tgcaagcact gttcgtctcc atctaacatt actggtgcac agtcttcttc cttccatgtc
    61261 ctgtaaatat aagctggata atgccctcgg cacatgacat cggagaaaaa ccatgaattg
    61321 cgtttaaatt gcagtgaacc aaatacattc tctggatcac agtcatatgc ataagtcgct
    61381 aatgcagaaa agctacacat aatacccatc ttagcatttg gatcgatttt ctttaataat
    61441 tgagtagcac gggcgttcgc tagaaacata tgatgacagg cttgataaat cacttttttt
    61501 gttagtgtag caatgggttc aagtttatct ctagcaggga atgtaaccaa tccagcagct
    61561 gcataaggca tacgaaaagc attattgatc tcattaaagg tcatccaata tttcactttc
    61621 ccttggtagc gttcaaagac ggtctgcgca taaaattcaa aaaagtcgat taattttcga
    61681 ttttgccacc ccccatactc tcctaccaat gctaaggggg tttcataatg actaagagta
    61741 attactggct cgatatcata tcgatttaat gtattgatta gtctgtcata aaatacgaga
    61801 ccagcctcat ttggcttctg ttcgtctcct cttggaaata ttcgtgccca tgagatgctt
    61861 gttcgaaaag cttttaaacc taactccttc agtaaacgaa tatcctcttc aaatcgatga
    61921 taaaaatcaa tcgcttcatg agaaagataa tggagtgatt cgtctaaagc aatctcccaa
    61981 atcttttttt cttcattcca ctgaatacta ggatgctggt tcatgatccc gataagcgta
    62041 tctgtcacat taggtaattt gccgtcttcc aaccatgcac cttcacattg attagcagct
    62101 actgctccgc cccataaaaa attttctgga aatatttcct gatccataac tatctctcct
    62161 cgctatcaac aatttcttac ttgaagtata gctcgattca tcaaaacaag atattctaac
    62221 tatttcggca gaagagaaga aaaaaattga atgctactct tcatttttaa tatttatata
    62281 taacaaatac ataagacgat ccccgaaaga aaacactcgt taaccatcta agcaaacgat
    62341 ttattttact tccaacacaa atgcttcata aggtcgtaaa tcaactttgc cgtctggtaa
    62401 ctgtttcaga ggataatttg acacaataac ccttaaagct tcacgtggaa tagtgaacgt
    62461 ctgcttttca ctggacatat tacaaataac gagccattgc tggtcttcat atatgcgaga
    62521 ataggcaaag acatgctcag gcgtcttttc cactaattca tagtctcccc agacgatgat
    62581 gtcatgtttt ctacgcaaat cgatcaattt cttgtacata taaaaaacag aatcttgatc
    62641 tgctaacgct tcttctgcat tgatttcttt ataatttgga ttgactcgaa gccatggaat
    62701 tccagtagta aacccagcat gttcacgggc gttccattgc ataggcgtcc tagcattgtc
    62761 tcttcctttc gccttgattg attccaagat ttcctctttt gtaaatccag aagaaatcct
    62821 ttcgttatac atattgattg tctcaatatc ttttgcttcc gcgatatcat cgattgggca
    62881 attcgtcatg cctaactctt ctccttgata gatataaggg gtgcctttca tcatatggag
    62941 caagatagca aacatcttgg cactttcttt tcgatacaca tcgtcatttc cccaacgaga
    63001 aacaattcgc ggaagatcgt gattattcca aaataaacta ttccatcctt catcacctaa
    63061 agatgtttgc catttggata atactttttt cagttcagag atactcaagg gtttcaaatc
    63121 ccatttgtct ttgccttctt gctgatctaa tcctatgtgt tcgaattgga agaccatgga
    63181 taattcattt cttttaggat tggaataaag tttagcgatt tcggtagttg ctccccatgt
    63241 ttcgccaaca gtcatcagat ttttgtcacc aaaagttgct tgattcattt cctgaagata
    63301 ctcatgcagt ttaggtccat tacttgtgat ttctttatcc ggttctttgc caatcatatc
    63361 gatcacgtcc atacgaaagc ccccaatacc tttatcgatc cagaaattca tcatttgata
    63421 aatctcttgg cgaacctgtt tatttttcca attcaaatca ggttgacgct tgctatacaa
    63481 gtgcagaaaa tattgccccg ttttttcatc caactgccaa gcagaaccac tgaatattga
    63541 gcgcaatcta ttaggctctt ttccattgac cggatcacgc caaatataat agtcccgata
    63601 aggattttct ttatttttac gggcctcaat gaaccacgga tgttcatcag aagtatgatt
    63661 caccaccaga tccatgatga tacggatatt tcttttcttt gcttcttcaa tcagttcttc
    63721 catttcttcc atcgtaccga attctgccat gattgcttga taattactga tatcataccc
    63781 attgtcatca ttcggagact cgtatactgg agaaagccaa attgcatcaa tacctaaata
    63841 cgccagataa tccaacctgg aaataatccc ccgcaaatct ccaataccat cttgatttga
    63901 atcttgaaaa cttcttggat agatttgata aacaactgaa tggtgccacc atttttgttc
    63961 catgattttc ctcctataaa tggatgtttc tattttttgc ttaatcattt attttagtct
    64021 ctctcactaa aaattgtaac gctttcaaat agctattgca ccttgttatc ggtaacagaa
    64081 taaaacaaaa aaacagctga ggaaattccc tcaactgttt tttgaaagga ttgatttatg
    64141 cagggaagaa tgctgatttc acaacttctt tataaacggt gttcactcag ctgtcccttg
    64201 gtattagctc aaaaaacggc aaaatataag aaactatttt ttcgtttttt tctctaatac
    64261 tcaggccaga acgctgattt cacaacctct ttatttcaca ttcaatatcc acatgaatcc
    64321 cacaaataca aagaacagac catacatgat tggagatact tcttttcctc ttttagctgc
    64381 taacatggta attggataga agataaaacc taatgcaatc ccatctgaaa tactataagt
    64441 caaaggcatg ccgatcaaga tcaagaatga agggatcgca atctctaagt ttccccattc
    64501 gatttgtcgc aaagattgtg ccatcaatac tcctaccata atcaatgctg gtgcagttac
    64561 ttgtgaagta acaactgata gtaatggaga aaagaacaag cctaacaaga agaaaatccc
    64621 tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg ataccagctg atgattctac
    64681 aaatgcacca acaggagaag taccaaataa ggaacccgca agcatagctg ttgaatcaga
    64741 agctaaagct ttccctacac gaggcatctt gttgtctttc atgaaacctg cttggtttgc
    64801 taatccaact aaagttcctg ctgtatcaaa gaaagtcaca agcaagaatg tcagcactac
    64861 gacccacatt tgaagagaat tgatgtcccc tacatgtttt aatgcaacta aaaatgttgg
    64921 tttcaaactt ggtgctgcgg caacaatttc agaaggcatt ggaatcaaac ctgtgaccag
    64981 acctaaaatc gttgtagctg ccatcccgat gaagatgcca cctggtacac gccgaacaag
    65041 caagatagca gttacgacta atccaaaaat cgtcaaccaa gtagaaccaa cgttgagtga
    65101 tcctaatcct actaatgtag attcattcga aacgatgatc ccaccttcgc tcagaccaag
    65161 gaaagcgata aatagaccga taccgccaga aatcgcatat ttcaaatctg ctgggatcgc
    65221 atcaatgatc atttcccgca gcttgaagat tgtgatcaaa ataaaaatca aagaagcaac
    65281 gaatacgccg gctaatgctg tttcccatgg aatccccatt cctaaacaaa cagaataagc
    65341 aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt ggataacggg ctaatacccc
    65401 cattaaaaca cagccgatcg cactggctaa tgcagtcgca gtaaacactg ctccttcatc
    65461 cattccagaa gcacctaata cagttggatt tacaaataag atataagcca ttgagataaa
    65521 tgttgtaaat cctgccagta cctcacgttt taccgtggtg ttcaattttt ctatttcgaa
    65581 gtaggaaagt attttttcct tcatcttcca tccccctaaa tccgaacatt ataaataaag
    65641 tccgttaaaa tttcgattta ttaataaaac aacgtgtttt atcatatact aaaacaaaac
    65701 ataagtaaac aaaaaattcg gattttaagc aaaatccgaa cgttccatct gattaaatcc
    65761 ctttatctca aagttcatga acattcactt tggattacaa gaatttcttt attgtgttaa
    65821 taaagtttct tcattctagt ctcaaaaatc aatagtgtga tttgatttta ccatttcgtt
    65881 tctgttcttc tttgatgaat tcttttgtat cgacaggatt cagattattt agtccgccaa
    65941 aacctgtatc gaccattttt ccccagcctt ttgtcttctt ttttcctgat ttgagattct
    66001 ttgctcgaag cttctcacta tgttttgctc gccgatactt caaataacct tctaatcttc
    66061 ccattagatc cactgttttt cctccttttt attcaatcag acgactcagc ttttcctata
    66121 acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca agatgccatt gttatctgtg
    66181 atttctcctg gttcttccat gttctcgtag atagtttcaa attcatccgt aaaaaatact
    66241 aaagaaggcg gtggtcctaa tacctctggt gaaaacttct gcacaaatac ttttgggaat
    66301 acgcctaatt ctaattcttt tgagatgctg agtacaatac tcttaaattc ttctggcaat
    66361 tcgattgttt cagaaatttc tgcaccaatt ttttctacaa agaatcggca aatcatctct
    66421 acgtcattga cataaagcat cacacgtgtt ttttgtatca tcatttgacc cttctttcta
    66481 tttgattttc tttcctgtct ataaactgat tccattatat cacgaaagcg caaacacctt
    66541 gtgtttgata ctattcaaga aaaataaagc aatagtttct aggattgctt tcctccacaa
    66601 acttttttta caaaaaaaaa acggcaatga atgccgctct tcttatttcg attcttttat
    66661 aattctgctt ctgattcatc tttggttagt gtgtgtctaa cactagttac gccaaatgtt
    66721 tgatcgattt tttccttcaa cgcttcgatt gcctgtgcgc cacgtgccgc ttgttcagca
    66781 ttgatggatt caatgaccag caccgctgct tgtgtttctt ccgtcagcat cgttctttgg
    66841 gcttcacgcc agttgagaca gcgacagatc gctccttgtt catcatagta gatgatttct
    66901 tccggaagtg ccggagcatc ggaatctgca cctaaaggca ggaatgattc gccaccttgg
    66961 gcttttccta aatgcagatt tcctgcgatc ttttcgatat cttctccgcc gcaaggtacg
    67021 ccaaaagaca aggaaatact attatagata tctacaagcg gattgattgg gaaaaattcc
    67081 cgttcttgat ttgcacgttt cagtaaagct tcaatagaag aacgtgcacc ttttttagtc
    67141 ttgaatgtgg taaatgcctg acgccactga gcaatgactt cgttttggct gaatgtttct
    67201 tctgtaaggt aatttcttgc ttcttttgct gcctcattca ataattgatg atacgtttct
    67261 tcactttctt ttgttacgtg gttatcgata ccatttacgg ataagatttc gatggttgct
    67321 tcaggaaata cttcccagaa attggtatcc actattactt ttttcatctg ttcacacctc
    67381 ttgattagaa taattgcttg atgaatagat agtctgtttg gggatcatct cccatataaa
    67441 aaacatgttc gctgtatttg ataaatcctt ttttacgata aaaagcgagc gcaacatcgt
    67501 tgtgttccca aacaccgagc caaatggctg ttttttgctt ttcttttgct ttttcaatag
    67561 ctgtatctag taaaaatgta ccgatacctt gatgtttaaa tttttttcgt acatagattc
    67621 gctcgatttc taaaaaatga tcagccattt cctcagattg actcgttcct gtatttagtt
    67681 tcaggtaacc cgcaggctct tgatcgatac tcactaggaa aaaatcggtt tctttatctt
    67741 ttagctcttc tgtaagtttt tctagtgcat aagcttgtgt caagtaatca gctaaatgct
    67801 ccggtgaatt atcttttcca aatgtatctg taaatgtttc gatactaatt tgttgtagta
    67861 agtctacatc tgaaggcgtc atttttcgaa tttcgtattt attcacggct tcttcctttc
    67921 aatagatcct tttattgcct tttttgacaa aattccaatc ttcgctaaca ttcttgcata
    67981 ctttatttaa aagatattcc aattgttttg cttcttcgtc agaaagacca tttaatgcga
    68041 ctgcattgga gtagttgttt tctcgaatga tgaaaggata gatttctgct ccttttttag
    68101 tcggatagag tttcttgatt tttttatttt ctttatcttc caaacgttcg atcatcccat
    68161 tggattctaa tttcttgatt gctcgggcag tcgttgtacg atctactttg atcatttctg
    68221 ctagtttttc ttggatgatt ccaggatttt cgcaaatacg tactaaataa agatactgtc
    68281 ctcttgttaa atcgacttct ttgaattcga tattgctaat agaatctagc gctcgggcaa
    68341 tcattccgat gtctcgaagg atttcagcca tttcatccca cctttatttt gtttgtaaga
    68401 ctagttcacc tctttttgtt gcaaatgcaa ctattttcaa taaaaaaatc aagttctttt
    68461 ttaataacta gtaaagtata ttcttttctt cttttacgaa aaagctcaat cacaatagat
    68521 ctggtttttg ttttctaaaa taagtaactg tttcttcgtt gttcgctttt ctgatagctt
    68581 tggcaactct tctatactaa aaaatcccat gttggaagtt tcattattct caataaactt
    68641 ggcctcttca ccgtggatcg cacaagcaaa aacaagttta taatactgaa aaagctgtgg
    68701 aatatctttt tgtttattcg tatcaaaaac tgctcgcaac tgagtagttt ctactgttaa
    68761 tcctgtttct tcatagactt ctttacgcac attttcttca ggagacaatc ctatttcagc
    68821 aaatccacca ggaagtgacc attctttcgt ccgtttatct tcgattagta ggaccttgcc
    68881 atcttttttg atataggcac gaacatctac ttttggtgtt ggatgtcctt ctccaatcgt
    68941 tgtgagcgct tttatttctt cgaaagtttc ttttcctatt tcactgatca gttccaatga
    69001 aatcgtccgg atctcctcaa aacgctcttt atcaaataca tcttttccat aatatagacc
    69061 agcttgagaa agtgcgagta gtcttttgta ttgagccaac ttcttgttca ttctcttccc
    69121 ttctttttta gcaaatgtat gactatgtaa tcaagtatag aagactggtt taattacggc
    69181 aaataaatat aatgaaatct gcaataaata aaagaatgtt aaaaaactcg agaacaaaag
    69241 acattcgact tctctggggc tgtgaataaa tggcgttcaa aagcttgcat ctgcggtaat
    69301 aagccccacc aaacgaaaaa tatgacgagc tattcttgtc tggctgttct tattaattga
    69361 ggcaggacag ctttttcaca acctccccac taaacggtgt tctcctacca actcctcggt
    69421 cacaagtcac aatattcatc aaacatgaga agctgttttt ttgaaattgc tccttgtgac
    69481 tcggagctga acgacaggct cacgacctct cggtaaacag cgttcaaaag ccaatttttc
    69541 gatattaagc catttctttg ttacttattg tcattgattt ctttttctaa tttatccaat
    69601 agtggagtgt aatccacttg agtatagtct gttacatcaa attcatatag tgttcctaac
    69661 tggaatttgt catattttcg gtcggcgatg atctttttga atgcttcttt cgtaatatga
    69721 ttggtccctt tcgttcgatc ttccaatacg tcaccatagt gataatgatc catgatatag
    69781 ctcaaatgac gatctgattc acgatcacgt ttgtaccgac gttgccataa cacttcaaag
    69841 tctgctgtta ggcgaatcgt gatcacctga tatccgtatc tatctgctaa ttcttggagt
    69901 cgtgcttttt gcttatcact aaaaggatac tcggacacaa tcactttctt tcctgcttcc
    69961 atatacaaat caagtgcttt gtaatagaaa ggataaactt tcgtcctttc ttgttccgcc
    70021 cgttcttcca aattgttaaa cccgatggat tctgcatagt attccttgat ttcatcgagc
    70081 gtgatggcat acatatctgg aaatcgttcc cgtagtttat tcataagata tgtttttcct
    70141 gtaccaggac taccagccag aagaatgaaa tactttttca tctcatttat ttcctaagtt
    70201 aattggactt tccaagttca taaacagtcc aatcttatgc ttgacaattt cttttactgc
    70261 ttcatttgct ggaatcacat tatggcgaag agacttgttg atttctgttt cactaaaggt
    70321 ttctttagct actttcgtcc agttgactaa aagctccgtt ccaacattga atttgcggat
    70381 cccgttattg atcaattcag gataatcttc ttctttcaca cctgttcctc catggataac
    70441 tagggggaca tcaactactg catcgatttc tttcagcaag ggaatgttta cttctgtttt
    70501 tgatttgaat tgtccatgat tcgtaccgat agcaatagct aatgcatcca cgcctgtagc
    70561 ttctacaaat ttcactgcgt cttccggtct tgtatatacc ttgtcatttt cagcgacatg
    70621 gattccttct tctgtaccgc cgattgttcc aagttctcct tctacggaaa caccgtgcgc
    70681 atgggcatat tctactactg cttttgtctt caggatattt tctttgaaag gcagatggga
    70741 cccatcatac atgacagaag tatatccgct gtcgattgct tctttgatat catcaaagtc
    70801 tcgggcgtgg tctaagtgca agactgcatc aaccatttct tcttctgcaa tagctgtcat
    70861 tacattgact aacacatgca tgccgatata tttagcggta tcgacacttg tttgaataat
    70921 gatcggcgaa cccatttctt ttgctgcgcc tagcatagca ggaagcattt ctaggttatg
    70981 cgtattaaaa gctccaactg taaaattcaa ctcttctgct gtttttgtta cttctttcaa
    71041 tgttgtatac atcttttatt gccaccttat ctttttattt gtttcctttg atcgttactt
    71101 gttttgcaat ttgtcgcatc tcatccattt tgcccatgta gtaatcgatc ccttgatcat
    71161 caccagcacg agcagcatct gcacgcttct ggttatagag gctcatcagt tttttatacc
    71221 catctccgat cgtctgttta ttttccaaac tcttttctaa atattggatg gcttgatcgt
    71281 tcacatacaa ttcagaaaat ttaagtccaa cttgttcata aagtttcgct aatttatgct
    71341 tatcttgggt agtggaaatc tcttgtttca attgtgtaat ctctgattga atcttttcgg
    71401 tcgcttctgg cgatagggct ttttcttctt ttgccagaac ttttttcggt tctttttctt
    71461 ttttcttaaa aaatccaaac atcattttcc ctactttcaa ttaaaatgct ttcaaaactg
    71521 ggcttaagat ccaggcatat aagagtgctg cggctactgt atctacatcg gttgctgtag
    71581 cattgttgaa tcccatcgaa ttaaagatcg ttactagcaa agcgggcaat aatgtaataa
    71641 agaaaccatg agcaataccg ccaatgatcg ctcctcggcg tccgccaact gcgttgccaa
    71701 atatccctgc tgtccctccg gcaaagaaat tggttagcat accaggtaaa atcatcgcta
    71761 agccgaacat gggcaaagta aacatggcaa taatcgtacc aatcgttgtc gtaatgaaac
    71821 ctaaaatgac agcatttggg ctataaggaa agaaaactgg acaatctaag gcagggatcg
    71881 catcaggaac taatttcata gctatccctc gaaatgccgg cacgatttca cctaacagta
    71941 gacgtacacc agctagcaat acatacacac ccactacgaa ttggatggct tgtaaaaagg
    72001 caaacatgat atagttttga tctcctgata attcagaagc gaatggttcg cctgcaaata
    72061 aaacagtgat gatatatagc gggaccatca cgaccatgac agacaaataa gtatcttgca
    72121 gaaattcgaa atgtgtcggc aacttgatat cttcgatcga ctttttattt tcgcctttct
    72181 caccaaaaag cttagcaaca ccggcttcaa ataagtaccc gatcgtacaa aaatgaccta
    72241 aagcaatatc atttgatcca gtgattttac gaatgatcgg ttgcgctact gccggcatag
    72301 caatggcgaa acaagctccg ataaaaccac cgaccaagat caatacgatc cctcgcagac
    72361 cggcaaaata gccaaagact gtcgtcatcg ttgccatcca taaaattgct tgaccagtta
    72421 aaaaaatgta cttccatttt gtgaaacgag ccaaaataat attgaataca aaaatggcta
    72481 agaacgtcag tgcaatatct ctacctaaac ccaaatcatt cattgcttga ccattgatag
    72541 attcaataga agggatgatc ccttgcatat gaaatccttc agtaaagatt ttcccgaaat
    72601 aagttaaact tccaacgatg atgctcgaac cagcactcaa cacttgaaat cctaggagtg
    72661 tctttaacgt gccagagatg acttgtcctg ctgatttctt ctgcagtagt agacccaaca
    72721 tagcaatcaa tgcaatggtg atcgatgcct gagtcaaaat attttcgatg ataaaattga
    72781 taacgcccat gtttttaccc tcttttcttt ttgtatttag ataactccct tatcttgcag
    72841 aactggggtg attttttctt caatttcatt ttttgaaaca atatttttca aatagatcat
    72901 cgccgtttta ggatcaactg cgaacttttc gaactgcgaa cgaaaatttt ctgctgtgat
    72961 gatcacatct ggtttcatgg aaactgcgga agaaatatca caatggtcca gtttggcttc
    73021 tactcctaat ttgttcaaaa catcctcagc agacatctgt gctgcaaaac tacttcctaa
    73081 acctgcaccg catacgaata aaatgttcaa tttgttcatc tttgttctcc cccgtatcat
    73141 ttattatttc tcaactactg aaaaaagcag ttgtttgaaa tcttcgatat ttgtgcattc
    73201 aatcaatcga tccaacttct cttcgttgtt gatcaattca attagttctt tcatgatgtt
    73261 cagatgcgag taggagtcta ctgctgccaa acaaaaaata atctttactg gatccatttc
    73321 aggattaccg aaatcgattg gttgctcaat cgtagctacg ctgactccta atttattgac
    73381 cccatcttct ggtcgggcat gggctagtgc catgtgtttg ccaatcacga tatacggtcc
    73441 gtaatcattc actgcacgaa tcatcgcttc gacatagcgc tcttcgacaa tgttttcttt
    73501 aaccaatggt ctagcagcta gttcaatcga ttcctcccaa gtagtggctt tttctttgat
    73561 catgatattg ctgtccgtca aaatatcttt taacataggc tggatctccc ttttgttgat
    73621 ttctaaattg tttgtttcga ataatttttc taacttgcca tagatgattc gatctactcg
    73681 gccaccactt tctttcatga catctattat ttgagtgaat aactccgtcg attcatgact
    73741 gttaggctct aaccgctgta gttcttcatt tcttttcaaa aagtcttcta taaccaagat
    73801 attcgtctca gttaaaatcg gatcgacaac taaaagcggc ttatttcgat aattcaattg
    73861 aaaggtgctg aaaaccagat tgacatccaa tttatctact aattcgattt cttttgaact
    73921 taaaactgca gtaatctcga tctgaggaaa cttttctttc agattctctg ctaacagatt
    73981 tccagtagcc atcccatggt tacagacaac gactgattta taaacataat tcaagtctcg
    74041 cttgattaca ctggcgatcg tcgaaaagtg gatgacaaga aaagctatct cgtcctccag
    74101 tatcttgcca ccaattactt cttcgatcgt aggaacaaaa tgagtgactg cgcggtagat
    74161 tgggaaataa tttgttcgga tgttttcttt caatggattc atgacttgga tatgattctt
    74221 gattcttaca atcagtgcag ccatgtgctt atagagattt tcgaataaag cttcttcctt
    74281 tagatggaaa ggtacgtgtg tctcgttctc tacaaattgg atcaattgga tcgttagtaa
    74341 ttgagcttgt acccactcga ttgaattgga gatatctcta gcattgaacg tctcaatagt
    74401 gaaacgaaga taatttcttt ctacttgcgg gacattgtcc aattgaaatt cctcgatcat
    74461 gtggtcaata aatgcagaaa agctactgtc ttttatttct gtatttttcc aattaatggc
    74521 agagataaat tcatgtttcc tgattcgctg gatccagatc aatgtaaaaa gcaggatttg
    74581 ttttttataa atatcatctt cttgtttcaa caacttattg gaatagatct cctgtatttt
    74641 tttgatttcc accaaaggaa aataatggag aaagattttt tgagccggtg ttaattgtgc
    74701 atcttgattc ataaagaagt cggtcattcc cagattctga ttgataaaat catagatcat
    74761 cgtacggata gatcgttctg aacctttata aaccagtcct tgctttccat aactgacgat
    74821 ctctatccca taactgacca gtgttgctcg taaccgtctc atatcttcat ctaatgtact
    74881 tttagagatt tgaaattctt cttctttttt gtttagatgg atcgggattc ggctgtaagc
    74941 aacggctaaa actagatcaa aaatacgttc gcgccgattc aaaaagccgt ctttcacatc
    75001 ttttatttca gtttctaatc gagcttgctt ttccttttcc agcttcaaca aaaaaccttt
    75061 gttgcgaatt gtacggatct ccggaagctt tttactgctc aaccagatat tcgcttcaag
    75121 aacttcctga cggatcgtcc gctgactgac attgaactca cttgctagat gttttgcttt
    75181 tactggatat tgggctgtca tgattctgtg aagtatttca attgttcttg ttttcataga
    75241 aatgatctcc ttctgatttc aagtatatgg ttccgcttac ataataaaaa gtagatattt
    75301 ctgccacttg aagtggaaag aaagtaagca aagcaaacaa aaaataagcc atatgctttt
    75361 tcatctcctc aacgaaaaag acatacgcct attattacaa aaattttgcc catctataca
    75421 aaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacaacta aactaaaata
    75481 gtgcttccta tttttcgttt ggttgttctt attatcgcag atgcaagctt ttaaacacca
    75541 tttagtggag aggttgtgac agagatgttc agccttgagc gataagaagc gattttgaaa
    75601 aatcgcttcc catattttac aaaatcgtgc cttattgccg ataggctatc tctggcacac
    75661 cgtttattca gaaaaaggag ctgcgacaag gcttttgtca caactccgtt cataaatatt
    75721 tcctgtcaat ccagcataat cggctgaata aaatcggctt gccatctttt ttctttctca
    75781 gaaaaatcaa aataccatat ctgagaaaaa tactgtcgtt gtgtcttact tcctgagatc
    75841 aatcgcccat cttttcgatc gactgaataa tcacagcatc tgaaatcaat tcggatggca
    75901 aaactatttt cgtggatctg tcgataattt cctacttcct gaatagttat ttttttagta
    75961 tgatttctta caccgtctgc tttgtttttt tgcagaactt tctgatgttc ggcaaacagc
    76021 ttgtctgtat aacagcttct ggctttttca ggagtttctt cttcccaagc ggattggatc
    76081 ttcatgaata tcttttggat ttccagaaac tgtgctttct tttctttagc cgttcctgac
    76141 atgcgggaca atatctccgc tttcagcctt ttcttttgtc ttttattctt gacgatagaa
    76201 aaagcagaaa cccctcctac tgacaaaaca gctaatgcaa gaaatcccgt ttgctcataa
    76261 ctagaaggac ctgtacctgg accataatac ccataagaat ctctgccgta agaacgactg
    76321 ccaaaataac tgcccccata tgaacgacca ccaaatgaac ctctgcccga acttcgaccg
    76381 ctagatgtac ctccaccatg accacccgcg acagcgaatc cttgggtcgg cagagcaaac
    76441 aaacataggc ccactaacaa aatgattgct aaataattga tttttttcat tgtcttcctc
    76501 gttatctttt attacaaaaa actattttta tgatagcctt gctaaaagga agagtcaata
    76561 accagatgca taaactgttt ttttagcaat tttgtcttat ttagtcattg agtaacatcg
    76621 gacaaatgta tgtgtcggca gtccataatt cctctatctt cagcatgaac aagcttgcag
    76681 acaagagatc tgcgcttcct ccaggagaga tttggttttc ctgacaaaag tgcaacagtg
    76741 ctgtaaatgg acgatttggt aaagcacttt gactagcagc atgagcaagt ttttgcattt
    76801 ccactaacgt tttctgatca ctgcgatgca atacgcatgt gtcatctagc gaactcatca
    76861 aaactaacaa catatgcatt tgtttctgta catatgaatc attatctagc tccttagctg
    76921 cttgaattgc aagcctgata tgcggatacc ctaatatcgc ttcttcatat gcaccattta
    76981 cgccatattt ttgcttcacc ctatgaccat gagtggtttt tttatgacga tattttgtat
    77041 caggaaaagc agcaagcttg ctgacatcat caagtatttt cgataaagaa aaaggctgtt
    77101 ccatactgat tttttgtgca atgacactga tcaatagccc cattgcccaa atcgctcctt
    77161 tatgggtatt gatgccattt gtagcaatca gcattgcctt ttccgctttt cgcccaatct
    77221 cagcaattct ttctcttaac tcctgatcaa tagcatgatg ccatgctatt tgagccattt
    77281 cttcaaaaaa aggagttagc gtcttagcag acttaagaaa taaagcatag tccatatcgg
    77341 aatgtgcacc cgcatccagt gcatccacta atccaggttt tggacttaat gttacttctg
    77401 cgatcaatgc ttcctctacc aacgcagcga gttgctgagt cactgctttt tttcgttcgt
    77461 catttatcat gacactcact ccttcttcct actattactt gctgaaacct tttttgatag
    77521 ctacccaaat gatatgaagt aaccagctta aagcaaatgc acaacttgcc gtcattagat
    77581 aagctacaag aatcatgact cctgaaggta actgcagcca actatcttta aacaataata
    77641 acagtacttg gaaaacaaat acattggcta aatacgtacg ataagcataa acagacaacc
    77701 atttgaacaa tggcaatttt ggcgaatcgt ttacaatcat tttggaagcg cctatgaaga
    77761 cagcaaagat gatgactaaa ctgtataaac tttggatcgt attcaagtaa ggtgcattcg
    77821 caaagctcaa gtctcctgga tgtgcgagca atgaacggac agaaaagaac gcgataacta
    77881 aggcaacagg taagaaaaga taacgtactc tgtccaaata ttgatagact ttttcgtgat
    77941 aaacaaatgc ggctacccca taaatcatga aactagcaac aaaccgatct aataagtacc
    78001 agctgtcatg ataaggccca gtcaatactt gtgtttgata gaacagatac catgcgacat
    78061 ataaaatcgt tgaaccaatc aatactggcc atacatattt tttattagca aatactttgt
    78121 aaccgagcca aacaaaaaac ggcatgaaca attggatctg cagcatcata acggtatacc
    78181 ataaatgagg cgctccatca cctgtgacat atttcagtaa aaagcttgca gtatccgtat
    78241 acggagtttg ttgttggact gctggaaata gccaaagata agccgtcgtc catagaagat
    78301 acggaacgac tagttcaaaa aacttttctt tcaaaaattc ccaataagac aaatgctgac
    78361 ttgtttttac catattataa acaattgcaa aaataaacat tggtgctgtg tatttaacga
    78421 tgacgtaaaa ggtaccaaga aaagctgtat ttgttgcgct gtggctgttt gccaaagcaa
    78481 agctcaatat cgtttgcatc ataacagccg ttacagctga tactttctgg aagtcactta
    78541 cgttgatttt gatttttttg ttttcaaaca tctctttctc tctctttttc tttattacca
    78601 aagttctttg gttaatttag gtccttcgac tgttttcaat agaatttttt tctctggctc
    78661 tctcaaatac tcttttagtg aaaaaccgcc ttgccgagtc actacttggg tatcgaattg
    78721 ctcaaaaaaa gcaggatgcg attgaattac ctgaattggc aattcaatcg gtgaatttgc
    78781 gtacagcagc aaatcaaaat cactggtttc ttttactgcc ggaatcccag tcgataattc
    78841 aaatcctaag ctgccgccga ttccccattt acatcctgat aatatgtttc tagcagcatg
    78901 aaattgctga taaacaggat aatccatgat tttctcttgt ctatacgatt cttgttcgat
    78961 gatgtcccac ggatgaatga cttgtttgac ggcatgtttt gagatttctg ttgaccatct
    79021 ctgattgcgg gcaggtccac gtaaaccaat cggtagtatt tctgcgtgat ccgtcgacgc
    79081 acgacgaact gttccgaaca tcttttcagt tagaacgacc cattcaggca aggtgaaccc
    79141 ttctatttca tgtttatcaa aaaggacaat atcatgtgct tgaatcattt tttatgcatc
    79201 ccattcttcg tccattttgg cacggacttt atttgtttcg acacgtccga atgttttggc
    79261 tgtttcgttt gtgtatcgat ttgtcagcat cgtgtcattt gtattgcgga catcttgaat
    79321 ggcttcattg attgcttcga tgactttgct gacattttgt tctgaagcag cttggtcggt
    79381 gatatcttct aggaaatgat agatcgctcc tagttttgta taattattga tatcgtaagc
    79441 gatagctgga actttttcag cagctgcttc gatttcagca accgaacgtt ttgtcacacg
    79501 tgcagcactt tctttagaca tcgcttggac ggtaatttct ttattgttta aagcaatcaa
    79561 gcggcttgat tgtaatccat gtgctaaaaa tgcacctgaa atagcatttc ctgcaatcaa
    79621 tccaataact ggatgtccag cttgacggag ttttgcataa gcagcagcac tattagccaa
    79681 agccacatgg ataccgatca attcttcgtt atagccgtat gcttggcttg gtacatcgat
    79741 cacaacgatg attggccgtt tcactttttt gtctttatct tcttcgtaaa cgtgatccaa
    79801 aatttgtccc atgcggaaac cttcaactaa accaacttca ccgtttcgta cacggtacat
    79861 cgggttttta ggatcaggta cgattgatgt cacgatggct tcttctatga atttacttga
    79921 tttaccataa tacacagtac taatagaaga agttggattt ttcatgccag ttagtgccgc
    79981 aaaccaatcg taaccaacac ttgctgcagc tggttcattt ccttctgcta cttctggtaa
    80041 gtcaattgat tttgcttgat ggttagcaat tgcttcattg taatcgtcaa tggccattgg
    80101 ttttgaaaga tctaaagaat caagtaatga taaataaaat gctgagcttt cactgcgatg
    80161 cgtgtctttt cgttcgtcca ttgctgcgat gacagcagct ttgattgctt ctacactatc
    80221 agtcactaat tcatcaatga tttttgattc tacccgttga cgagatccta atgtattcca
    80281 aatcaagtct ttgtcactag aatcaaattc tcgaacgcct gcttcttgtt caataacttc
    80341 tggtccattc aaaccgactc tcgctttttt tgttgcgatt aaataactca ttaatgctga
    80401 agtaatcgac ataccgccaa atgatcctac acgtcctgga atcaaaccga tgactggtac
    80461 atattgtttt agagcaatga ttacatttcc gatttctgaa atagataaca gtccgtagtt
    80521 tgcttcttgc aaacgaacac cacctgtatc tagtacaatg actggaaaga tctcatttcc
    80581 ttttttattt tcttctaaca catgttcaag tgcagcaacg atttttgcac cggaaacttc
    80641 accgataccg ccaccttgga attttccttc catagcgatt actactgctt gtttgccatt
    80701 gatcgtgcca cggctgacaa cgatcccgtc atcactttca gggacgattc cttgtgctgg
    80761 taaacttggc gcgatgattt gatcgaatgg atcaatcaat tcacgtccat tgtcaagtag
    80821 agcgttcact cgttctctgg cgtttaattc tacaaaacta tttttcatct gaaagttcct
    80881 ccattgcttg cgtcaatcgt aggtacacaa ctcctggtgt agcaccgaag tcattgatca
    80941 cgatatctgc tgaaattgga taacgatcaa aaaatctcgt taatacattg ttccatactt
    81001 cgccgaagcc atcgcttcct gtacaaattt ttacagtaga ttctgttttt tctgttggaa
    81061 acatcaagat ttccaaatct cctgaaccga ccactcctac atggattttc ttattgatcg
    81121 gctgtttcgt tggatattta taagctaatt gttccatgga tctctcccct ttttgtaaaa
    81181 cagagcatac acgttctgct tattcgtctt tcttcttcca tctgggtaaa tggcgacaaa
    81241 aggtttttct cgccaatttt atgaccaagt tctgaatttc attggtggtt catacaggcc
    81301 atctgaccat tcgatcaaat catcgatggt ttgtgcagct aataatgaac gtttcgcttc
    81361 actacgtttg atgcctagat cttctggtaa tttcacgatg ccggctgcac gcaagctatc
    81421 tagtgattct ttcgtgctag tcattccaag aggtgtaaca ccgccaattg ctgcaattgc
    81481 tgcttgtcgt tcttcttttg tttttgcttt gtacaaataa gcaattcctt cttctgtcac
    81541 gatatgtgta gtatcttcgc tataaatcat cactggtgca ttggctaagc ctgcttgttt
    81601 ttgaacagct agtgcatcta attcttcgac aaagactggt tttttatttg aaccatatgt
    81661 ttcaaccatt tgaacaacta atttttggcc tttccctaat ggatcatcat tgtcgcgaag
    81721 tgattgccat gctggtgtag aatgacgacg accgccagga ttatgtccca tgttaggcgc
    81781 accgccaaaa ccagataaac gtccttttgt tacggtagaa gagtttcctt cgtaatctaa
    81841 ttgcaaggtt gaaccgataa acatatcgat ggcatattgt cctgcagctt gtgataatgt
    81901 tcggttactg cgcatcgtgc cgtctttacc gacaaaaaag acatctgggc gagcagcaat
    81961 atatttttcc ataccaactt cgccaccaaa actatggatg ctttcaaccc aacctgattc
    82021 gatggctgga atcatcgttg gatgtgggtt caatacccag ttcttcgcga ttttcccttt
    82081 taaccctagt gattcaccat aagttggcag taacaattcg atagctgctg tattgaatcc
    82141 aataccgtga ttcaaagatt gtacgccgtg ttctgcgtag attcctttga ttgccatcat
    82201 tgccattaga atttgcaatt ctgtgatgtt ttgaggatct cgtgtgaata aagcttctaa
    82261 ttgataaggt ttatctgctt tcacgacaac gtctacccag tcaccaggga tatctacacg
    82321 tggtaaagaa tcgacaactt cgttaacttg aacgatcacg ataccatctt tgaaagctgc
    82381 agcttcgatg attgttggtg tttcttccgt attgaaacct gtgtacaagt tgccgtcttt
    82441 atctgcttta tctgcagcta ctaaaacaac atttggaatc aaatcgatga ataagcgtcc
    82501 ataaagttcc aaataagtat ggatatcacc taatttcatt gtgccgtctt caatcatttg
    82561 ggcgacacgc aagctttgtg caccagcata agagaaatcg atcttgcttg cgattccttt
    82621 ttcaaaaata ttcaaatgtt ctggtcgtga gatactagac atgatcaaat gcagatcatt
    82681 cactttttct gcatctactt tttcaagact ttctgaaaga aaacttgctt gtttttgatt
    82741 gtcgccttct aatacaactt tgtcgccggg agcaatcagt ttttccatta cttctacgat
    82801 tttttcagtt tctacatact tgccattgag caagttgctg attttttcca atcgctgcgc
    82861 ttttttttcg cgtttagaag cccagttgcg gtttggtttt tccataatta gtcatctcca
    82921 tttttgatat agaaacgtgg cgtcatcaat accatgttgg tctagattga tcgtacggat
    82981 ccctttatct acaccaaatt ttgtatgaat cagctttgtc aaagtattgc cggggggaaa
    83041 ttcaatcgtc agatccattc ctaattcttt tgccaagtcc atcatttggt tccactgcac
    83101 agggaaagct aggttttcca acaaatcttt acgaatctct tctgcgtcct gagtggctct
    83161 tccatccgta ttttttatat acaaacatct agcatcagat agatggacag actgcatata
    83221 cggcttgaat ttcttgaccg tatcattcat caagagacag tgagatggca cagggacttt
    83281 cagcagcttc gctgtttgcg cttggtgccg tttggcttct gccaacgtaa cggataaacc
    83341 ttcgatactc ccagataacg tatgttgcat cggacaattt tcatttgaca gataaactgg
    83401 atggtcagga tcaaatgttt ttgctacaat ttcctcagct tcgcttctcg tcacacctac
    83461 tactacgccc attgcataac cgcttggata agcttctttc attaagactg cgcggtgata
    83521 gactagttcc gctgcattct caaatctcaa agatcgacaa gcaacagctg cacaaaaagc
    83581 accaatcgaa tgtcctgctg caatatcagg ctgaataccg tttttttcca tttcatctaa
    83641 ataaaatact gcttttgtca gtaatgctag ctggataaaa accgtatctt gatagttttc
    83701 ttctttatca actaaccgat accccgtcac attttctaaa cgttccatgt acatttcagg
    83761 gatatccgct aacatcccta acttctgact gccttgtcca ggaaataaga aacctgtact
    83821 catgagcctc gcctcctcag cacttacttt ttccactgct atcttagtca atttctgcta
    83881 taataaaaaa tgccaaaaaa agaaaaaacc ataacaaaaa ggtatggatg ggatggataa
    83941 ttatgaaatt gcttcatctg cagtatttta aaaaagtatt cgaaacaaaa aatgttaccc
    84001 aagcagccaa agaattattt atttctcaac ctgccttgag ccgtgcgatc aaacatttgg
    84061 aaaacgaatt aggtgtccct ttgttttacc atagcggcag aaacattgag acgaccgttt
    84121 atgcagaaga attttatcca tatgcagtca agacattaga cacattagaa gaaggaatca
    84181 aattattgtc ttcggtcaat gagaagatcg ttacttccgt catattgtat ttagaggtag
    84241 cctctgtttc tattccgaac ttggtccgaa tttttatgga aaagcatcct gatatccaat
    84301 taacgatcat gcaacatcat ttaccagatg atattaccga tcctgtactg tacatcactt
    84361 cggagatgaa gcctggcatg accaatattc ctatcatgaa agaacctgtt tatgtcgcca
    84421 ttcctaagaa acatcctctt gctaaaaaag aacatctgca attagaagat atccaacgca
    84481 cccccatctt gatgctgtca aagagcaacg ccttccgtca tacactggat gcagcacttg
    84541 aagcgaaaag tatcgaattg acgatcagtt cgacgacaga tgatcctgcg acattacgtt
    84601 ctattgtcaa gcaaggatta gctgttagct ttttcccaaa agtttcttgg tcttacgata
    84661 aaaatgatcc tttcgtcttg cgggaaatca aagatttgcc cttgacacga acaatttatt
    84721 tatcttctag cttcacagaa gatcatcctt tgatcaaaat gattgcaaaa acgatgcggg
    84781 aattttattt aggaaaataa aaaaaaaaga gctgggacaa aattcttgtc gcagctcttt
    84841 tttcgaacaa acggtgttcc agaagtaatt tcttagaaaa taagccgaaa tttcccaaaa
    84901 atttgagaag caattttcgt aaattccttc ttatttcttg aagctaaaca tttctgtcac
    84961 aacctcttct ctattgtcct agacattcat tgcgttgatt taaaacttcc atcaatcaaa
    85021 tctgctagtt catctagctt ttctatttta cgtagatcaa tagagatcca atgttcttta
    85081 ttcatgtgat aggctggaaa atacccctct tttcctcgca aaattccgcc aagttcagga
    85141 tcgaccttca aatcaagaat attgatgatc ctatggtcac ttgcatcatc atagattttt
    85201 tcttctggaa cagacatcag gaccccaaac cattttttgc ttttttgatg tctgaatacg
    85261 caataatcag gaaatttttt aaaaatatat tctggagaca aattgtatgt ttcattgatg
    85321 tgatcgatta gttctttttt cgttagcatc tgcttccatc tctttcttat tgattcgctt
    85381 tattcaaaga taccacgtaa gaatagaaaa atgaatacaa gtagttgcta atcaaatact
    85441 tcgatatttt tcttttttat caactcttaa atgttcaatc cgcctgtgtt ggtcttgcta
    85501 ccatctacta ttaaaatgtt tattattgtt tgcccaccta ttttttgatt ttagtggtat
    85561 catttttcaa gttcatgatg aaattcttat catcagtaaa tgctccagaa aaataaacaa
    85621 tgattgaaaa atcagttgaa acttgaccac cgtttatttt ctccgtattc ttagaataga
    85681 agggagtctg attgatgtat tcactaatga aaaaaatcat taccgaaaaa gatattcgac
    85741 gtcatgtttc tttagtagaa caattgctga atcatacaaa gataaccgta aatgaattag
    85801 ctgaaatcat agggacaacc gaacgaacga ttttttctga ccttcaatct attcgctctc
    85861 agctgcccga aggctgggac atcatcagcg atcaagcagg aatcagtttg caaaaccagc
    85921 aaaatttatt aacaaatgac ctttgggaga tttttttcaa acaatcagtc agtgtcgaac
    85981 tgctgaaaaa tctgcttttt acaaaaaaag ttgctgtccc tgattttcta gccgaccatg
    86041 ggctttctta tgggacattg aaaagacatg tgactaaaat caatcagcgt ttagcaagtt
    86101 acgacttgca gatcgatttg acgaaatata cagcttgtat tcttggaaaa gagcgtgcga
    86161 ttcgtacttt ttatcatcga ttgctgattc ctttcactca taacaactat tttttcgagg
    86221 attattcgat ccatgaatcc cattattttc aatttttaag aaacctcagt caaactgaac
    86281 tagcggttga aacagaagag attttcggaa catgttggtt ctttatcaat acgatccgta
    86341 taaaagcgaa ttgccgatta gattcttcta tccataccaa cagcacactc tcctccctgt
    86401 atgactctgc attgaaaaaa ttgtatttaa cggaaggtat ttatcttaaa gacactgagc
    86461 tttcttttgc ttctttctgc tttctggaaa gttggaatta caacaataac tatggacaag
    86521 aaaccgctcg ctgcctgcat cattctccat ttttagaggt cctcgaaacg tttgtagagg
    86581 aactagcttc agagctgtct cttgatcaac taaaaaaaac atctctgact gataacttgg
    86641 ctctcctcat attaaaatat catgaatcgc cgatattgat tgaagcattg gaccgtcaat
    86701 accatcgttt tttagaaaca tatgatcagc aatatgcaca cttgtatcaa cagaaagaga
    86761 atttactgaa cgatctaaaa aaagaaatag aaatcaatga accaaattac tttctccaac
    86821 tcttgtctct actgatccaa caaacgattt tttctatcaa accgaatttg ttcaatgtct
    86881 attttatttt ccaaggagaa ccttcatgga aggcgttctt gcagcaggaa ttaaaagatt
    86941 acttaggaaa acgtgtctgc ttcttaccta tcgaactaac cgatctttcc actatttctt
    87001 ttgaaaaaac agatatcttg atttccaatt tccctctaga ttctctagat atccctattg
    87061 tatacatttc ttctattcca acgaaaaatg agctcaatcg tttgactgaa ctcactttca
    87121 aaactttttt atagtaacgg ctagcgactg tattctctag ctgttttttc ttttttgcga
    87181 aacagtttgt tcatgatccc tttttctatt tctacaacta agaatagaag aatcacattt
    87241 gctccaatca ataactgttg tgtcatcgtt aaacttactg taccgatcag ctgctggccg
    87301 actggtagaa aaataaccat cccttgcaat aaagcaagga tacctaatga aagaaaaagc
    87361 gctttgtttt gcaaaagacc ttggttcaat gacggcttgg acaactctct gcaattgatc
    87421 atgtaaacag cttgagctaa gacgatattt tgcagaagaa tggtttgctg taagctttgc
    87481 ccttcaaact gcatagccaa taaataggca ggaaccatga tcagtaggga gacatagatg
    87541 atccgaaaca aatgataacc agataggatt ccttgctttg gatctcttgg tgggcgcttc
    87601 atgatttctg gatctggcga ctcgaaccct agtgcataag ataaagtgat tgtcgttacc
    87661 atattgaccc ataaaatctg tactggggac aaaggtagcg ggcgattcag cataagtgcc
    87721 caaatcaaga tcaatccttg agctaatgca gttggcaaga agaaattgat ggttttttgc
    87781 aaattatcca aaatccgccg gccctctttt acggcttttg caatcgtatg gaaattatca
    87841 tctcctaaaa ccatatcagc tgcctgtttg cttacttcac tacctttgat tcccatagca
    87901 attcctacat cggatctttt caaagcaggt gcatcattta cgccgtctcc ggtcattccg
    87961 acgatttctc catttttctg caaagcttta acaattcgca gtttatgttc tggtgtcgtt
    88021 cgagcaaaga catcgactat tggcacttgc tgaatcaact cttcatcaga cataagatcc
    88081 aaatcgatcc cttctaaaac tttggcagta tgttttaagc caatttgttc cgcaatcgct
    88141 tttgctgtgt ctttgtgatc ccctgtaatc attttcactg agatccctgc ttgttggcat
    88201 tcctttacag ctttgactgc actttctttt ggcggatcga tcaatccggc gatccctgca
    88261 aaagttaatt gggttaaagt ctcatgcgtg agttcctgtt tcgaatctac ttttttataa
    88321 gcaaatccta aaacacgctg accttttctc gcaaattctg cagctgtctg actccatgct
    88381 tcttgttctg cagaagaaag agttgattta gcaaataata cttctggtgc acctttgacg
    88441 taaatcaccg ctcctttttc ctccgcatgc atcgttgcca tatatttgta ttcagaactg
    88501 aacggaatct ttgcttctat ttttttcaaa ggtagattgg ctgtttctgt gtgtcgaatc
    88561 agcgcaactt ctgtcggatt gccacttaaa ttttctgttt tctgttcttc ttttgttttt
    88621 aattcctgac agtttttcat aatggacaac acgcaggctt cttcttttgc agctacatcc
    88681 acaactgtca tttcattttt tgtcaatgtt cctgtcttat ctgaacagat cactgtcata
    88741 gaacctaatg tttctaccga aggaagtcct ttgatgatcg ccttttcttt tgccatttca
    88801 tgtactccaa cggatagaat catggtcaaa acagctggca atccttcggg taccattgac
    88861 accaccaaag caatcgtagc cgaaagaaga atatgccaat ccataccata tcgaaatgtc
    88921 gtaaaaataa ctaagaatag gctcaaacaa agaatgcctt gaaaaatttg tttgtttaat
    88981 cggttgattt ttttaatcag aggcgtcgtt tgttgttcga ccgattgcaa tgcttgattg
    89041 attttaccga tttctgtcgt gtttcctgtt tctacaacga caccgactgc agaaccagat
    89101 tgtacaagcg ttcctgaaaa acccatattt ttctggtcgc cgatcgattc ttcttctgtc
    89161 aaaacggcat gacttttttc agatgcttct gattctcccg tcaaaatcga ttcttccact
    89221 aataaatcat gtgcttcgat taatcggata tctgctggaa cgacatctcc ggcacgtaag
    89281 acaatgacat ctccttttac cagttctttc gcagggattt tttcttgcat cccgtttgat
    89341 agtactatcg cttcttgtcc catcagctgc ttcaaaccat tcaatgattc ttccgctttt
    89401 ctttcttgcc agtaactaac gagtccattg acgatcacta cgaataaaat aatacttcct
    89461 tcaatatatt cactagaagc aaatttcaaa aaagaggcaa tgatcaacac gatcatcagc
    89521 agatcagtaa aatgctttgc tatttttttc catcgttttg tctgctgcct tgcttttatt
    89581 tcattatatc cttgatcagt caggcgtttc tgtcgttctt cttgcgacaa gccttctgtt
    89641 gatgtttgat agtttttcaa tacatcttct accgtcaatc tgtaccagtt catcctatcc
    89701 cctcctgttt aattgatact gttattatac gcagggaagt tttttcaggc gtatcgatta
    89761 tttcaggggg gtctgaaata attcgttttg caaaatgtat ctgggcctaa ctgatatttc
    89821 tattgcttat tcttttatat acaaaattta agaatacaaa ccagataacc aataaaaaag
    89881 gaataaagcg tgtcgtgctt tcgaataaca agctgaaagc aattgaagta aaaaacacca
    89941 acaaagcaag atctttatat ctttgtgtag tgttccgttt cttttcttta ataaattgaa
    90001 catgtgcaca caagatcgaa gaccacacaa atagaaaaca actcgttgtc atgcctgcca
    90061 aaagctgaaa aaacaatcga ctatcagcta caaaaaaatg tagacaaaga ccggcaaaaa
    90121 ataacattcc tgtaattaaa ataccgtgta aaggtacgcc tttcttagaa agcgcacaaa
    90181 accaatgggg caaacttttt tcttttgctg tttgatataa aatcctgcta gtactgtaga
    90241 ctgcactatt tgctgaggac aaagaagcaa ccaaaatcat tagattgaca attgctgctc
    90301 cgccttggca atggatcact tgcagcatag tcactaaagg actttctttt aaatctaaac
    90361 aatgccatgg aacaacaaat aaaatcgaca aaatcgttcc tatatagaaa aagagaatac
    90421 gccaaggcag acagttgatt gcttttttca aattcttttg cggatcactt gtctcgccaa
    90481 cggttaagcc gatcatttca attccagcaa aactgaataa aaccatagga aatgagacta
    90541 ccattccagc aaagccaaac ggtagtaatt catcgatctg tccaaaattt tctttgattc
    90601 cgatagactg ggtatgaaaa tctgagacac tcaatcctat acccaataag ataaaaagga
    90661 aaatccccaa gatcttgatg attgagaaaa aagattccaa ttcaccaaac cacttgactg
    90721 caacaaggtt gactgccatt aagatcatta gtgcgctacc tccgcttatc aaagctggta
    90781 catgtacaaa ccagaaatgg atatacaatc ctattgctgt taattccgca agtccagctc
    90841 caagccagca aagccaataa gtccaattga caaaaaatgc ccactttttg cctaaataat
    90901 agtcaacgaa atcactgata gagttaaatg tactatttgc taaaaataaa tcacccatgg
    90961 cccgcatcaa accataaata gccaaaccgc atatcaaata aacgagaata acagctggcc
    91021 ctgctgatgc aattgccttt gctgaaccta aaaataatcc tgttccaatt gcgccaccta
    91081 atgcgattaa ctggatattt cgattagtta gagtccgttt cattgccggt ttttccatca
    91141 agttcacctt tttctttttc aagcagcatg gcgacttcct gttcccaaga aggtttcttt
    91201 attttttcaa tacagagtgg tactacaaac aaaatgacac tgatggctgt gactgcaatt
    91261 agattgatcc atgttccagc tttcatcgtc agtccgacag aaaatccgat tgccgcaaat
    91321 aaaacaataa cgacagtcca aactccaaga ttgctctttt tctctttttt tccaattcta
    91381 aatggtcgat tcaactttgc atgtgtataa cgcaatctca aaactgcgat ccccatgatg
    91441 acatagacga tacagtagat aacagtagtt gaatttacga gcatcaaaaa tgcttgattg
    91501 acgccaggaa tcagcaaata aaaaagtgca aagattgaga taatgacaga ctgagtcaaa
    91561 ataacggatt tggatacgtc aaattgattc gtcttccaaa aattgaattt tggtggatac
    91621 gcaccttttc tagcactttc tgtcactgtc tttgctggtc ctgaggccca agcagataat
    91681 tggactgcta caccaatgaa gacaagtaaa ctaaacagat tcacaataag atgtggcaaa
    91741 ccaagaattt ggcaataaat cgaaatagat tgcgcgatat tattcaattc catttgtcct
    91801 tttggtacga catttgctac aactaaagca ttcaaaatat tgacgaaaaa tataaaaatt
    91861 aacgcggcaa atactccgcg aatatacgtt ttaactggat tttccaatcg agtgatatac
    91921 acagaggaca tttcgatacc agtaaaaata aacatgatcg gcgcaaaata aacaaatgag
    91981 gatgcagtcg tagtatctgg tattaatttg tcccatgaaa aactgcctaa tgttccggtt
    92041 ggtacgatcc caactttgat ccacgcagca aaccctaata agagcatcat cgtcaaaggc
    92101 acgtacaatc ctaaccaaac accgactttt ccgccgattt ttgccatatc aaatttcaaa
    92161 ttcaaaaaag tgatgatcca ataaacgact aaaatgacaa ctaatgtaaa tttgctgttc
    92221 aaaccaagcg gcacattatc gataatatta ccaaacaacg gagcgagtgc tgaagcgacc
    92281 atcaccatcc caggaaacat ttggacccat aacagccatg agacaacgaa cccccatttt
    92341 tttcctagag cattcgatac ccaaagttct ggaccacctt tttggggaaa cattccagca
    92401 aattcaccag agatcaatga gattggaaaa gcgtacaata atactgcaac cagcatgtaa
    92461 aaaatcatcg tccagctcgt tgctgctaca tctggaatat ttcgtacact tgctacaagg
    92521 gcgcaggtca ttccgataaa ggtcatggtc gtgatttgct ttgatgaacc agtatctttc
    92581 atctttattc cttctttcaa tagcgacata tgttttt
//
LOCUS       DACDYM010000003        76090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDYM010000003 DACDYM010000000
VERSION     DACDYM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.003"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..411
                     /locus_tag="IWM59_000219"
     CDS             <1..411
                     /locus_tag="IWM59_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9978284.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="IWM59_000220"
     CDS             509..697
                     /locus_tag="IWM59_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9978285.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="IWM59_000221"
     CDS             complement(755..1108)
                     /locus_tag="IWM59_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9978286.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="IWM59_000222"
     CDS             1639..2061
                     /locus_tag="IWM59_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9978287.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="IWM59_000223"
     CDS             2058..2288
                     /locus_tag="IWM59_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9978288.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="IWM59_000224"
     CDS             2786..2986
                     /locus_tag="IWM59_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9978289.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="IWM59_000225"
     CDS             3013..4206
                     /locus_tag="IWM59_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9978290.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IWM59_000226"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="IWM59_000226"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9978291.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="IWM59_000227"
     CDS             complement(5898..6821)
                     /locus_tag="IWM59_000227"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9978292.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="IWM59_000228"
     CDS             7017..7622
                     /locus_tag="IWM59_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9978293.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="IWM59_000229"
     CDS             7930..8946
                     /locus_tag="IWM59_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9978294.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="IWM59_000230"
     CDS             9087..10388
                     /locus_tag="IWM59_000230"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9978295.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="IWM59_000231"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="IWM59_000231"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9978296.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="IWM59_000232"
     CDS             11117..11515
                     /locus_tag="IWM59_000232"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9978297.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="IWM59_000233"
     CDS             11581..12663
                     /locus_tag="IWM59_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9978298.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="IWM59_000234"
     CDS             12875..14437
                     /locus_tag="IWM59_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978299.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="IWM59_000235"
     CDS             14430..15563
                     /locus_tag="IWM59_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9978300.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="IWM59_000236"
     CDS             15563..16519
                     /locus_tag="IWM59_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9978301.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="IWM59_000237"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="IWM59_000237"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9978302.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="IWM59_000238"
     CDS             17808..18626
                     /locus_tag="IWM59_000238"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9978303.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="IWM59_000239"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="IWM59_000239"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9978304.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="IWM59_000240"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="IWM59_000240"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9978305.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="IWM59_000241"
     CDS             complement(20506..21087)
                     /locus_tag="IWM59_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9978306.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="IWM59_000242"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="IWM59_000242"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9978307.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="IWM59_000243"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="IWM59_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9978308.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="IWM59_000244"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="IWM59_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9978309.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="IWM59_000245"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="IWM59_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9978310.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="IWM59_000246"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="IWM59_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9978311.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27136..27444
                     /gene="rpsJ"
                     /locus_tag="IWM59_000247"
     CDS             27136..27444
                     /gene="rpsJ"
                     /locus_tag="IWM59_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9978312.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27475..28104
                     /gene="rplC"
                     /locus_tag="IWM59_000248"
     CDS             27475..28104
                     /gene="rplC"
                     /locus_tag="IWM59_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9978313.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28132..28755
                     /gene="rplD"
                     /locus_tag="IWM59_000249"
     CDS             28132..28755
                     /gene="rplD"
                     /locus_tag="IWM59_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9978314.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28755..29045
                     /gene="rplW"
                     /locus_tag="IWM59_000250"
     CDS             28755..29045
                     /gene="rplW"
                     /locus_tag="IWM59_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9978315.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29084..29917
                     /gene="rplB"
                     /locus_tag="IWM59_000251"
     CDS             29084..29917
                     /gene="rplB"
                     /locus_tag="IWM59_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9978316.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29960..30238
                     /gene="rpsS"
                     /locus_tag="IWM59_000252"
     CDS             29960..30238
                     /gene="rpsS"
                     /locus_tag="IWM59_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9978317.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30261..30608
                     /gene="rplV"
                     /locus_tag="IWM59_000253"
     CDS             30261..30608
                     /gene="rplV"
                     /locus_tag="IWM59_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9978318.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30622..31278
                     /gene="rpsC"
                     /locus_tag="IWM59_000254"
     CDS             30622..31278
                     /gene="rpsC"
                     /locus_tag="IWM59_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9978319.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31281..31715
                     /gene="rplP"
                     /locus_tag="IWM59_000255"
     CDS             31281..31715
                     /gene="rplP"
                     /locus_tag="IWM59_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9978320.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31705..31893
                     /gene="rpmC"
                     /locus_tag="IWM59_000256"
     CDS             31705..31893
                     /gene="rpmC"
                     /locus_tag="IWM59_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9978321.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31918..32184
                     /gene="rpsQ"
                     /locus_tag="IWM59_000257"
     CDS             31918..32184
                     /gene="rpsQ"
                     /locus_tag="IWM59_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9978322.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32242..32610
                     /gene="rplN"
                     /locus_tag="IWM59_000258"
     CDS             32242..32610
                     /gene="rplN"
                     /locus_tag="IWM59_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9978323.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32647..32955
                     /gene="rplX"
                     /locus_tag="IWM59_000259"
     CDS             32647..32955
                     /gene="rplX"
                     /locus_tag="IWM59_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9978324.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32983..33522
                     /gene="rplE"
                     /locus_tag="IWM59_000260"
     CDS             32983..33522
                     /gene="rplE"
                     /locus_tag="IWM59_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9978325.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33541..33726
                     /locus_tag="IWM59_000261"
     CDS             33541..33726
                     /locus_tag="IWM59_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9978326.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33762..34160
                     /gene="rpsH"
                     /locus_tag="IWM59_000262"
     CDS             33762..34160
                     /gene="rpsH"
                     /locus_tag="IWM59_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9978327.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34192..34728
                     /gene="rplF"
                     /locus_tag="IWM59_000263"
     CDS             34192..34728
                     /gene="rplF"
                     /locus_tag="IWM59_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9978328.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34889..35245
                     /gene="rplR"
                     /locus_tag="IWM59_000264"
     CDS             34889..35245
                     /gene="rplR"
                     /locus_tag="IWM59_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9978329.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35266..35766
                     /gene="rpsE"
                     /locus_tag="IWM59_000265"
     CDS             35266..35766
                     /gene="rpsE"
                     /locus_tag="IWM59_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9978330.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35781..35960
                     /gene="rpmD"
                     /locus_tag="IWM59_000266"
     CDS             35781..35960
                     /gene="rpmD"
                     /locus_tag="IWM59_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9978331.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36004..36444
                     /gene="rplO"
                     /locus_tag="IWM59_000267"
     CDS             36004..36444
                     /gene="rplO"
                     /locus_tag="IWM59_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9978332.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36444..37739
                     /gene="secY"
                     /locus_tag="IWM59_000268"
     CDS             36444..37739
                     /gene="secY"
                     /locus_tag="IWM59_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9978333.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37801..38448
                     /locus_tag="IWM59_000269"
     CDS             37801..38448
                     /locus_tag="IWM59_000269"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9978334.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38639..38857
                     /gene="infA"
                     /locus_tag="IWM59_000270"
     CDS             38639..38857
                     /gene="infA"
                     /locus_tag="IWM59_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9978335.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38890..39006
                     /gene="rpmJ"
                     /locus_tag="IWM59_000271"
     CDS             38890..39006
                     /gene="rpmJ"
                     /locus_tag="IWM59_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9978336.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39024..39389
                     /gene="rpsM"
                     /locus_tag="IWM59_000272"
     CDS             39024..39389
                     /gene="rpsM"
                     /locus_tag="IWM59_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9978337.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39417..39806
                     /gene="rpsK"
                     /locus_tag="IWM59_000273"
     CDS             39417..39806
                     /gene="rpsK"
                     /locus_tag="IWM59_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9978338.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39886..40824
                     /locus_tag="IWM59_000274"
     CDS             39886..40824
                     /locus_tag="IWM59_000274"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9978339.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40852..41232
                     /gene="rplQ"
                     /locus_tag="IWM59_000275"
     CDS             40852..41232
                     /gene="rplQ"
                     /locus_tag="IWM59_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9978340.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41504..42343
                     /locus_tag="IWM59_000276"
     CDS             41504..42343
                     /locus_tag="IWM59_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9978341.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42319..43188
                     /locus_tag="IWM59_000277"
     CDS             42319..43188
                     /locus_tag="IWM59_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9978342.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43178..43975
                     /locus_tag="IWM59_000278"
     CDS             43178..43975
                     /locus_tag="IWM59_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9978343.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44178..44924
                     /gene="truA"
                     /locus_tag="IWM59_000279"
     CDS             44178..44924
                     /gene="truA"
                     /locus_tag="IWM59_000279"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9978344.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45257..47926)
                     /locus_tag="IWM59_000280"
     CDS             complement(45257..47926)
                     /locus_tag="IWM59_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9978345.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48164..48352)
                     /locus_tag="IWM59_000281"
     CDS             complement(48164..48352)
                     /locus_tag="IWM59_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9978346.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            48622..48984
                     /locus_tag="IWM59_000282"
     CDS             48622..48984
                     /locus_tag="IWM59_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9978347.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49036..50718
                     /locus_tag="IWM59_000283"
     CDS             49036..50718
                     /locus_tag="IWM59_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9978348.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            50835..52871
                     /gene="recG"
                     /locus_tag="IWM59_000284"
     CDS             50835..52871
                     /gene="recG"
                     /locus_tag="IWM59_000284"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9978349.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            52920..53921
                     /gene="plsX"
                     /locus_tag="IWM59_000285"
     CDS             52920..53921
                     /gene="plsX"
                     /locus_tag="IWM59_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9978350.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54043..54285
                     /gene="acpP"
                     /locus_tag="IWM59_000286"
     CDS             54043..54285
                     /gene="acpP"
                     /locus_tag="IWM59_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9978351.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            54553..55557
                     /locus_tag="IWM59_000287"
     CDS             54553..55557
                     /locus_tag="IWM59_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978352.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            55558..56502
                     /locus_tag="IWM59_000288"
     CDS             55558..56502
                     /locus_tag="IWM59_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978353.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            56502..57464
                     /locus_tag="IWM59_000289"
     CDS             56502..57464
                     /locus_tag="IWM59_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9978354.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            57491..58405
                     /locus_tag="IWM59_000290"
     CDS             57491..58405
                     /locus_tag="IWM59_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9978355.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            58431..60212
                     /locus_tag="IWM59_000291"
     CDS             58431..60212
                     /locus_tag="IWM59_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9978356.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            60560..61246
                     /locus_tag="IWM59_000292"
     CDS             60560..61246
                     /locus_tag="IWM59_000292"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9978357.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            61266..64847
                     /gene="smc"
                     /locus_tag="IWM59_000293"
     CDS             61266..64847
                     /gene="smc"
                     /locus_tag="IWM59_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9978358.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            64856..65665
                     /locus_tag="IWM59_000294"
     CDS             64856..65665
                     /locus_tag="IWM59_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9978359.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            65676..66674
                     /gene="ftsY"
                     /locus_tag="IWM59_000295"
     CDS             65676..66674
                     /gene="ftsY"
                     /locus_tag="IWM59_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9978360.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            66861..69128
                     /gene="gshAB"
                     /locus_tag="IWM59_000296"
     CDS             66861..69128
                     /gene="gshAB"
                     /locus_tag="IWM59_000296"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9978361.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(69217..69669)
                     /locus_tag="IWM59_000297"
     CDS             complement(69217..69669)
                     /locus_tag="IWM59_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9978362.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            69819..70769
                     /locus_tag="IWM59_000298"
     CDS             69819..70769
                     /locus_tag="IWM59_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9978363.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            70762..71721
                     /locus_tag="IWM59_000299"
     CDS             70762..71721
                     /locus_tag="IWM59_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9978364.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            71718..72473
                     /locus_tag="IWM59_000300"
     CDS             71718..72473
                     /locus_tag="IWM59_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9978365.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            72496..73449
                     /locus_tag="IWM59_000301"
     CDS             72496..73449
                     /locus_tag="IWM59_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9978366.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(73972..74268)
                     /locus_tag="IWM59_000302"
     CDS             complement(73972..74268)
                     /locus_tag="IWM59_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9978367.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            74623..74979
                     /locus_tag="IWM59_000303"
     CDS             74623..74979
                     /locus_tag="IWM59_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9978368.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            74989..75888
                     /locus_tag="IWM59_000304"
     CDS             74989..75888
                     /locus_tag="IWM59_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9978369.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        24796 a        13751 c        16323 g        21220 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt
    26821 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
    26881 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
    26941 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
    27001 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
    27061 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
    27121 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
    27181 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
    27241 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
    27301 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
    27361 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
    27421 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
    27481 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
    27541 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
    27601 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
    27661 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
    27721 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
    27781 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
    27841 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
    27901 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
    27961 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
    28021 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
    28081 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
    28141 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
    28201 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
    28261 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
    28321 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
    28381 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
    28441 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
    28501 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
    28561 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
    28621 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
    28681 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
    28741 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
    28801 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
    28861 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
    28921 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
    28981 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
    29041 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
    29101 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
    29161 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
    29221 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
    29281 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
    29341 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
    29401 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
    29461 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
    29521 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
    29581 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
    29641 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
    29701 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
    29761 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
    29821 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
    29881 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
    29941 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
    30001 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
    30061 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
    30121 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
    30181 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
    30241 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
    30301 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
    30361 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
    30421 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
    30481 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
    30541 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
    30601 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
    30661 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
    30721 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
    30781 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
    30841 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
    30901 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
    30961 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
    31021 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
    31081 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
    31141 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
    31201 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
    31261 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
    31321 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
    31381 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
    31441 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
    31501 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
    31561 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
    31621 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
    31681 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
    31741 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
    31801 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
    31861 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
    31921 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
    31981 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
    32041 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
    32101 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
    32161 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
    32221 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
    32281 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
    32341 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
    32401 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
    32461 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
    32521 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
    32581 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
    32641 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
    32701 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
    32761 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
    32821 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
    32881 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
    32941 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
    33001 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
    33061 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
    33121 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
    33181 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
    33241 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
    33301 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
    33361 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
    33421 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
    33481 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
    33541 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
    33601 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
    33661 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
    33721 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
    33781 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
    33841 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
    33901 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
    33961 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
    34021 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
    34081 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
    34141 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
    34201 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
    34261 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
    34321 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
    34381 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
    34441 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
    34501 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
    34561 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
    34621 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
    34681 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
    34741 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
    34801 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
    34861 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
    34921 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
    34981 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
    35041 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
    35101 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
    35161 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
    35221 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
    35281 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
    35341 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
    35401 gtagggtttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
    35461 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
    35521 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
    35581 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
    35641 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
    35701 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
    35761 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
    35821 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
    35881 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
    35941 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
    36001 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
    36061 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
    36121 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
    36181 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
    36241 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
    36301 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
    36361 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
    36421 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
    36481 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
    36541 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
    36601 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
    36661 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
    36721 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
    36781 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    36841 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    36901 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    36961 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    37021 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    37081 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    37141 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    37201 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    37261 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    37321 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    37381 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    37441 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    37501 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    37561 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    37621 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    37681 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    37741 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    37801 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    37861 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    37921 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    37981 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    38041 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    38101 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    38161 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    38221 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    38281 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    38341 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    38401 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    38461 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    38521 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    38581 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    38641 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    38701 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    38761 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    38821 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    38881 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    38941 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    39001 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    39061 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    39121 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    39181 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    39241 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    39301 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    39361 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    39421 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    39481 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    39541 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    39601 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    39661 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    39721 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    39781 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    39841 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    39901 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    39961 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    40021 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    40081 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    40141 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    40201 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    40261 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    40321 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    40381 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    40441 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    40501 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    40561 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    40621 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    40681 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    40741 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    40801 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    40861 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    40921 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    40981 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    41041 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    41101 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    41161 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    41221 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    41281 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    41341 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    41401 tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt
    41461 ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc caattattga
    41521 attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt
    41581 ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa
    41641 atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt
    41701 gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatgat
    41761 ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg
    41821 tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga
    41881 gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa
    41941 acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga
    42001 agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat
    42061 caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa
    42121 tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga
    42181 aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa
    42241 actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga
    42301 aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg
    42361 aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt
    42421 tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat
    42481 gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat tcagccggat
    42541 acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca
    42601 gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt
    42661 ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg
    42721 gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta
    42781 gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca
    42841 ggacttgatc cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa
    42901 cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat
    42961 tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt
    43021 caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca
    43081 gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta
    43141 gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg
    43201 tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc
    43261 cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt
    43321 ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg
    43381 aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac
    43441 aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat
    43501 gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac
    43561 gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa
    43621 agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt
    43681 gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga
    43741 ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt
    43801 atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta
    43861 caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat
    43921 ggttatcatt gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt
    43981 cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt
    44041 gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc
    44101 tgaaaagaaa gaacgaaaaa attttataga aaaatcatta ttccagataa ccttttagga
    44161 acaaaggaga gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt
    44221 ttaatggatt ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat
    44281 tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg
    44341 gagtacatgc gatcgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac
    44401 gaatgcgttt cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa
    44461 ttgtttcaga agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag
    44521 tggatatagg gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc
    44581 cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca
    44641 cctctttttg tgcatcagga agctcgatag aggataaaat acgtacgatc tacgaggcta
    44701 agatggaggt aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct
    44761 tgtataagat gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc
    44821 ctgaagatag tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag
    44881 cacaccctga aggactttat ttgtatgaag tgaaatatga ataagaggtt atgatagaag
    44941 tggtcagctt caagaaataa gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa
    45001 tttcggctta ttttcgaaga agctacttct ggaacaccgt ttaagaaaga ggtcgtgagc
    45061 ctgtcattta actctgagtc acaaagagca atttcaaaaa acagcttctc atctttgatg
    45121 aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt acctattttt
    45181 tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttggtgcgga cagtctgaac
    45241 aggttccatc gtttctttat tttctacgtc ttcttctgtg tgtgtttcgt ggttgttctt
    45301 ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg gtcaaggtac cagacataaa
    45361 gaataaacaa tgcaatacat aggacaaaaa ggattgcacc tatcgtaaat ccttcaggca
    45421 agtaactgaa agtgacagta tgtttacctg ctggaacttt tatcatcaag aatgcgtctt
    45481 tgaaattgct gaccttgact ggtttcccat caattttagc tttccatcct ttgtcataag
    45541 gaatagttgt caccaacatg gaatcttcag cggtatttac agtcccagaa gcttttctgc
    45601 cagtggcttc catttcgaca cctttttctt tgatctgatt cattgcggct tgataggcta
    45661 caacatctag accaacgact ttaggctcca tgaaacttat ttctttcgtc ccataaaaac
    45721 ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc aagatcatag tattgaccag
    45781 aaatgttgat ttgcgttttt cgcgatgtac cattgacagt gatcgtcgca ctagaacttt
    45841 ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc ttgtgtattt gcaggcacat
    45901 ggacttccca agtgagctct tttggctggt tatctgctgt ttctgagtaa gtgacgccag
    45961 tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa agtaggtgta tagaatgtaa
    46021 aatactgttc atttagacca gacaatgtat tgatcaagtt tgtctgactg gtcaaattat
    46081 ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc agtaaatgct agtggcaatg
    46141 cattgacatt ctgatacagt gtatagtcgc cgctttgacc agcttggcgg aagccatatt
    46201 tactgaacgt gctttcgttt ttagaaatgt tatatttgat tcctgtaaac ccatccatca
    46261 gcaaagtatt attgttataa cggatattca aatttgtccc tcttgaacgg aaacctaatt
    46321 tatccaggta agaggaagaa ttacgatttc ttatagaaga aaacaaactg ataccactgt
    46381 aaccatagtt gatcgaatcg tttgcagata ctggatccag attttccaag cggtaaaacg
    46441 agtcggaatc tttttttgtt tcatttacaa gagctttcaa gtcaggataa ggatcagtat
    46501 ataaactgcg ggaagcataa ttccaatcat caagaatgcc atggatcata ctgttcgtat
    46561 tgaccagtgc ttcagcagac atgatcaata ataacaatat actcagatga ttgaatgaaa
    46621 gttttttatg ctgataaaat cccacagcaa aagcataaat ccctaagaaa accactgtca
    46681 atacaaagga agtcatcgtc acataggtgt agcttccttc aggtttgatg ccccatgcca
    46741 ccgcaaagag agcagctacg accaaaatga ttcctaacaa cagcccacca tcgtcttgtt
    46801 ctatcttttc ccatcctttt cctgcaagca ggagcactaa gaaagagaac agataggcat
    46861 aacggaataa aaacatattc ggtgcatgca taccatgcca gaacaaattc aatggaacaa
    46921 gataaaaact tgcgataagt atggcgaaaa gactgccgta caaaaattta tttttcaacg
    46981 gtactttccg actggcaaaa taaaacagac agaggactaa tggtagcaga cctgcgtaaa
    47041 cgaacggaat cgatccgtat tttgtcgtgt cataaacacc aatcatattt ttcatgatta
    47101 gatccagata agaagtcgct tctgttttca acgtagtgat ctgactcagt gcctcgccat
    47161 ttgaccgaag atccagcact gctggcaata caatgatcat tgaagcgccg cctgccagta
    47221 aagaagtgat accataagga acaatcgttt ttttatattc tgaccagttt gtcaatagac
    47281 gggcgataaa atataagaaa gaaaatacgc caatcataaa acccatataa aaactggata
    47341 aaaacaagag taaataactg ataaataaaa gtttcggccg tttaagatcc ataacacgat
    47401 gaatccccaa aatcactaat ggaagataaa caaatgcatc cagccacatg atgatctccg
    47461 aatgtgcagt gataaatgag atcaatgcgt aactgacgct aagtgcaaca tgcccccagc
    47521 gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa tccggcacag ccgaccttaa
    47581 gcaaagtcag aacatacagt gcatctggca tcatctggtt tggaaaaaag aacaccagtg
    47641 gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa ataattcaat cctagagaag
    47701 cattccatgt ataaaaaata ctttgctttc caagaagaac atttcggaag ctggcatgaa
    47761 aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact tctgctgctt ccaggataga
    47821 ttccgatact tagataaaca agtgctaaaa tcaaaaacgg aatcaggaaa ctagctgctg
    47881 cgtagcgtcc gttttctcta aagaatgact ttaaattttt tttcatattt attttccacc
    47941 aaatttctat gaagttgtat tcagtttatc atatccttgt ttttctctgc ttaaaaagag
    48001 tctagttttc acaaaaaaat aaaatttaga ataaataact tcataatttg ttcgtataac
    48061 aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca cccttaaaaa tttttattaa
    48121 ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg cgttcaactt
    48181 ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg tcgaccataa
    48241 cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata gcgtgtgaac
    48301 ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc atggtaattt
    48361 cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac taacatatca
    48421 tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg agaagaataa
    48481 ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac tttgggtaac
    48541 tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg ggagtttttt
    48601 acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg aaccattgaa
    48661 attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt gttcggtgtt
    48721 gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt gcgtaaagaa
    48781 aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt agatgtttat
    48841 acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca agaaaaagtg
    48901 aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt ctttgtacaa
    48961 ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca gtcgatctga
    49021 aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca ggaaatggtc
    49081 caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt aaatgtattc
    49141 cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag tggagctaag
    49201 gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct ttcaaaagga
    49261 ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt tcgtggattt
    49321 tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc agccttcact
    49381 catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac gattttgaca
    49441 gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga tgatgtcgtt
    49501 gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac tccagacttg
    49561 cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact gctgtttgtt
    49621 tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac atatgaacct
    49681 tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt ccaaggtcaa
    49741 atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa aatcggagaa
    49801 ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt agatggtata
    49861 ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt gcatacagaa
    49921 aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa aatcaaagtc
    49981 gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt gaacgagttt
    50041 gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc tggtaaaggc
    50101 gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg acaaacaatg
    50161 aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga taaagtgatt
    50221 gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga agtagcagat
    50281 atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc tatgctttca
    50341 ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat gttgtctagc
    50401 gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg tgtagagatc
    50461 aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa cccagatatc
    50521 acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga gatcgtaacg
    50581 atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga gaaactaaca
    50641 gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt ctatccttat
    50701 ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta ttcattcata
    50761 aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt tgttaaagga
    50821 gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca ggagtcggag
    50881 aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac ttactgactt
    50941 attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc caggatcaag
    51001 aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga tttggttata
    51061 aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac gtttcttttt
    51121 ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc gtatacggta
    51181 aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct caaacaatgg
    51241 atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca ttagttgatc
    51301 taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg ccaaacaacc
    51361 ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg cattttccaa
    51421 aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag ttctttttat
    51481 ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat ggtttggaga
    51541 tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt gaactgacaa
    51601 atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg cagcatatgc
    51661 agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg attgcgttat
    51721 acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa atcttggcac
    51781 aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc atcgcattga
    51841 ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta gagcaagggg
    51901 aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa tttcagaatt
    51961 taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg aaagtacttc
    52021 gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt cctagaacat
    52081 tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt ccagctggac
    52141 gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta ctggattgga
    52201 cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg attgaagaat
    52261 cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt gaactatttg
    52321 caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa aaagaagtaa
    52381 tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact gtaattgaag
    52441 taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat cgatttggat
    52501 tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct tcctactgta
    52561 tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc atgacagaga
    52621 ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct ggtgaagtat
    52681 ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg acggatggaa
    52741 atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag gattggcaga
    52801 tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag gataatcgtt
    52861 atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga gtggaaacaa
    52921 tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt gtagaaggcg
    52981 tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga aaagaagcag
    53041 aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca gacgaaaaaa
    53101 tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca tcaatggtcc
    53161 ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca gggaatacag
    53221 gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc gaacgccctg
    53281 gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat atgctggatc
    53341 ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt ttaggttctt
    53401 tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta aacaacggaa
    53461 cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt cagaatgaaa
    53521 caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt gtggctgatg
    53581 tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa ggaacagcaa
    53641 tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa ggaaaaatgg
    53701 gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat tattccaaac
    53761 atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat ggagcaacag
    53821 gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag acagatgtag
    53881 ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat atctgaagcg
    53941 atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa ttagccatgg
    54001 tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg cgaagaagta
    54061 tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga taaagtgacg
    54121 gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat ggaattcgtt
    54181 ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga acaaattgaa
    54241 acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca aataaaagca
    54301 aagaaggata aaaagctgca tgcagctttt tatccttctt tttttaatct ttttgacacg
    54361 taaaagtcat ttttctgata attctggcga taaacagaaa ttatgttgca aagtaaagcg
    54421 gttttctata taattattaa taattcatta cacaaagata agaataacct ttgttataaa
    54481 acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta ggaagcagag
    54541 aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca tacaggattc
    54601 cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt agacaaaaac
    54661 gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc tactacgatt
    54721 atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata taacgatctg
    54781 aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga tatcggaatg
    54841 attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga ccagatcaaa
    54901 gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg tgcactagaa
    54961 ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata tccccatgaa
    55021 ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg caaacctcca
    55081 atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc acagattctt
    55141 gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat cactcatgac
    55201 ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg acagttcgtt
    55261 gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac acgctcttta
    55321 ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat cgaaggtgtc
    55381 gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg tattccatgg
    55441 ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc gaaccacttg
    55501 gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga ggtgtagatg
    55561 acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag tggattcttc
    55621 aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat cgaaaaagga
    55681 aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg aaaagccgtt
    55741 gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga tgtaacgaag
    55801 cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt ccaagattca
    55861 atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc catccgaaat
    55921 tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct agatatcgtt
    55981 gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg acaacgtcaa
    56041 cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc cgatgaacct
    56101 gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa acgaatccag
    56161 caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt aaaacacatg
    56221 tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac aagagaagac
    56281 atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat tccgcaaatc
    56341 gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca agagtatatc
    56401 caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg tacgatcaca
    56461 actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa acgattctgc
    56521 gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc ttcttattag
    56581 cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag gatccggcag
    56641 tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc cagtacttcc
    56701 gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac aaacgctctg
    56761 ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc ttgacagttg
    56821 tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat caaaattcaa
    56881 tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct ttatttatct
    56941 tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca acaagtggat
    57001 cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt ttgaaatata
    57061 ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc caatatctgc
    57121 gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt tccaaagggg
    57181 taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg ccgatggctt
    57241 ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc gaaaagattt
    57301 ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg gactataccg
    57361 tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca ttactttcag
    57421 atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa tcagaaagga
    57481 gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc ctcctatggg
    57541 gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt cattaggttt
    57601 gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc aagacaaagt
    57661 gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga cacaaggaca
    57721 aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat tgatcatagg
    57781 tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga tcatcggggt
    57841 tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct tgatgcggat
    57901 cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg tatcgattat
    57961 ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt gggtagctaa
    58021 agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg tcagtgcctc
    58081 aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc cgaatctaag
    58141 ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta tcgagacaac
    58201 tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta cattgatcgg
    58261 ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac ccgcatcaat
    58321 ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca agcgttctgc
    58381 agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga atgaagaaaa
    58441 cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc gggggcggag
    58501 gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag gaattaactt
    58561 ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta gaagctgcgg
    58621 tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa gattcatatg
    58681 atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac gatttcagaa
    58741 tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa gcgacaatca
    58801 aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac gatgtgattt
    58861 tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac gacactttcc
    58921 gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc tccggaatca
    58981 aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca agcatgcttc
    59041 aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa ggtgtagcaa
    59101 tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt ggcccttatt
    59161 acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag cactactgga
    59221 atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca agtgcaactg
    59281 aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat acttacgata
    59341 cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct tatacttatc
    59401 taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac aatccagatt
    59461 caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat aataatgcag
    59521 taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt cctccagtat
    59581 tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac aaagcgaaaa
    59641 aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga gaagacaaag
    59701 atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca gcgcaaccat
    59761 tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa tacacaacag
    59821 gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat cctgaaattg
    59881 atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga ctatggggac
    59941 caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa ttgatcaacg
    60001 atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat caaaaatggc
    60061 aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat gaagtattgc
    60121 cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac ccatgggcaa
    60181 atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa aaagggcgat
    60241 gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag gtcaaggcgc
    60301 tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa
    60361 atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa
    60421 acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg
    60481 accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc
    60541 cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa
    60601 catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa
    60661 tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt
    60721 tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa
    60781 attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga
    60841 atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac
    60901 gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca
    60961 gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg
    61021 tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg
    61081 tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt
    61141 ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga aatcgaaaaa
    61201 attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga
    61261 aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag
    61321 tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc ggaaaaagca
    61381 atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg
    61441 gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag
    61501 aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac aatgagatca
    61561 gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct
    61621 gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa gaatcgttct
    61681 caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg
    61741 ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac
    61801 agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg
    61861 aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa
    61921 aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag acagcaaaaa
    61981 aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat
    62041 ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa aatgcgcaag
    62101 ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc
    62161 agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc
    62221 aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc
    62281 aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa gctgaagcaa
    62341 atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattattgg
    62401 cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta ggaaacgaac
    62461 tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag acgttagcca
    62521 agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa gaattgacac
    62581 aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc
    62641 aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat
    62701 taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa
    62761 actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg
    62821 gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg
    62881 aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc
    62941 aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga
    63001 caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt
    63061 ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa
    63121 acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa
    63181 cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat
    63241 ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag
    63301 aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa
    63361 aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc
    63421 gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctacaaaaca
    63481 tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg
    63541 aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa
    63601 cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa
    63661 gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag
    63721 atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag
    63781 tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga
    63841 cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat
    63901 tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag cagcgagata
    63961 gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa
    64021 aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt
    64081 tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc
    64141 aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag
    64201 aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca
    64261 gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat
    64321 tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg
    64381 aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg
    64441 aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac
    64501 ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga gcacttacag
    64561 ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt atcttagatg
    64621 aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt
    64681 tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag
    64741 atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga
    64801 tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat
    64861 caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga
    64921 tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac
    64981 aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg tagatgaagg
    65041 tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat
    65101 ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt
    65161 ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag
    65221 gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag
    65281 cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt
    65341 agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac
    65401 aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt
    65461 attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg atgaggaaaa
    65521 tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg ctgtgccttt
    65581 cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt
    65641 gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat aaaatcaagc
    65701 gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag tacgacaagg
    65761 gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt gccaattttc
    65821 gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt gcagatgtag
    65881 gttttgaaac agcgatacga attaccgaag cattacgtca agaagtgaaa ctcagaaatg
    65941 cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat ctctatgaag
    66001 cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta acagttattt
    66061 tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt gcccatcagt
    66121 tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc gctggagcaa
    66181 tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt ggcaatgcag
    66241 gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca gagcaggcag
    66301 atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg atgaacgaat
    66361 tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa gaaacacttc
    66421 ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa ttcaaagaaa
    66481 caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag ggcggtatcg
    66541 tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt ggtgaaggaa
    66601 tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc aaaggattac
    66661 taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg cagctccttg
    66721 tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta tacttatttg
    66781 taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata attagattca
    66841 tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat gtcaatgcgc
    66901 gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg gttgatcttg
    66961 caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc tatcacccct
    67021 atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt accgattcta
    67081 ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt tctataccga
    67141 aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa gacgaagaga
    67201 tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat ttagcaaaag
    67261 aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa tttggcgatg
    67321 agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc gaattcaaaa
    67381 cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg atcacttatt
    67441 tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca catccgcaag
    67501 aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca aatgtgaaag
    67561 tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc gaagaaggga
    67621 aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga aagaaagtcg
    67681 cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat ttgaatcctt
    67741 atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc ctgatgtaca
    67801 tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt acgactcgaa
    67861 ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa gaaggtagag
    67921 aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat tctctttatt
    67981 tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca gcgaaactct
    68041 atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga catcaatatt
    68101 ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg gaactttcta
    68161 ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta ttagatgagt
    68221 cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa aatgcaaaca
    68281 tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca gtaacgaaaa
    68341 aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca tccatggaag
    68401 aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa cctaaatcaa
    68461 ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa gattatcaag
    68521 caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa tttatgccgg
    68581 gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg ttgcgagtcc
    68641 ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa gaaaaaaata
    68701 gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa ttaggtgaat
    68761 tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc caagccaatc
    68821 aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg atcgatatga
    68881 ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa gcactaggag
    68941 ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct acaactgata
    69001 aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg catgtttatc
    69061 cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta tatcctgaag
    69121 tattttaaaa gaaaaagctt ttgacagatt gtcctatttg atgtcaatct gatcaaaagc
    69181 tttttttgca tgtaaaaatt tatgtgtttt ttcgttttaa aataaacgat cccagaagct
    69241 ttttttcttt tttggttttt ctttttcagc tggcgaagag ttttcttctg ggtatttttt
    69301 ttcgatatca atcacaggtt catttactgc ttcttttgct gttaagagca atccgaattc
    69361 ctcaggattt gcagtgacgt aatcgttgac tacagtgaaa cggctattgt ttttcgttgc
    69421 tagctggata tattggtttt ggacagaaag gggtagagcc ccattcaaga gtatggaagc
    69481 atctggatgt tttttgagtt cttttaaaaa gttttctttg ttttttggtt ggatcatttc
    69541 agcaacagtc atactaagat agcagcgttc cctgaatgtc cctaaatatt tatgctgttc
    69601 ttcagggttc actaaaggtg taccataaag tcccttatcc aaatggcttt ttacatcatc
    69661 ttgtgccata aaaaataccc tccttcttct ctttgtcttt cttacgatta tagcatattc
    69721 ccaataatac ctaaaacata tgaaaaagcg gttgcttttg ttcaacgatt cacttaaaat
    69781 aagaatgaaa atcattctca attagaaagg tggattggat gcgctacgga cgactagtag
    69841 cattattagt tattttaagt agtatttcat tgttcatcgg agtatcgaat gtacaattgt
    69901 cagaactgct ccagttagat gggcaaagct tacaaatctt gctagtgact cgtttgccac
    69961 gaacgatttg tttgatcctt gtgggagcga ccagtagtat ttgtgggttg atcatgcaac
    70021 atttgaccca aaataaattt gtttcaccaa cgactgcggg cacgatggat agtgcgcgtt
    70081 taggtatttt agtggctatg attttcctac ctggtgcatc cttgattgtc cgatccttga
    70141 cagcattttg ctttgctttt gcaggaacat tgcttttttt atcccttacg cgattgttcc
    70201 tccagaaaaa tcaattgatg cttccgttga ttggagtcat gttagggaat atcattggtt
    70261 cagttgctac tttttttgct tatcaattcc agttggtaca aaatatgact tcatggcttc
    70321 aagggaattt tgcgactgtg atacgaggaa attatgagtt attgtatgct gttgttccat
    70381 tgttgctctt ggtttggcta tttgcttacc gatttacgat cattggtctg ggcgaatcgt
    70441 ttgcagtaaa tctgggaata gattaccaga agatgcagtt tttaggcatt ggcttagtat
    70501 cattggctag tgcagtcatg ttgatcatgg taggaagcat tccttttatc ggagtgatca
    70561 ttccgaatct tgtctctaga atatatggag atcaagtgca caaaacaatt ggcgtaactg
    70621 ctttagcagg tagtttgttc ttagtatttt gcgatcttgt cgctcgcgtg ttgatttttc
    70681 cttatgaaat accagtcagt gtgatcgttg gaatcatcgg tggggctatc ttcctttatc
    70741 ttctgataag gggacgacga tatgcttaaa aaaaagccat ctatttttat tggtatgctt
    70801 gttcttgttt tatttgttac aggaatgtac ttgacgatcc atacatatgg aaactggcat
    70861 tttgcatgtg cattgaggag taagaaaatt tttgcattta tccttgtctc gctagctgtc
    70921 agcagtgcca caatcagctt tcagacaatt acgcataacc agtttttgac tccttcagta
    70981 ttgggacttg agtcgttata tgtccttgtc caaacggtcc ttttcttctt tatcggagga
    71041 gtgacgatgc ttcagcagac aggtacagtt tttttcttat tgaacgttgg gctgatggtc
    71101 gggctgagtc tattgttatt tttctttctt ttgaaaaaaa tgagagcgaa tcttttttta
    71161 ttactgatgt cagggatgat tttaggtacg ctttttggta gtgtcagtac ctttttacaa
    71221 gtcatcatgg atccgaatga atatgatttg cttcaaggca gattatttgc aagctttggc
    71281 aatatccaga gtcagtatct tttgcctgct tctttgatca ttatcggctg ctggtacctg
    71341 ttatttcgat atcgacatgt gctagacgtt ttccatctcg gaaatgaaca agcagtaaac
    71401 ctcgggatag acacagatcg ttttgaattt ttgattttat gttgtgtcag tcttttgaca
    71461 gggacatcaa cggctcttgt gggtccagtt acttttttag ggtttattgt cgcaaatatc
    71521 agttatcgtg tcttttctac ttatcgtcat ggtcagctat ttatcggaag ctttctggta
    71581 gctatgctgt ttctgacagg aggacagctc gcagtagagc agattttcgg ctggaataca
    71641 acagtgagtg tcgtcgttga atttgtcgga ggaatttatt ttgtcacaaa attgattcgc
    71701 gaaaggaaga gtatcctatg attgatttac aaaaggtcaa taaactgtac catggaaaat
    71761 cagtggttaa agatgtacag ttaacgattc ctgaaaagaa attgactgct tttatcggtc
    71821 caaatggtgc tggaaagtca acggtgttgt cgatgatcag tcgtttgata ccaaaagata
    71881 caggtgaaat ctatttagac cataacgaag taaaggcttg gaaatccaat gaattagcca
    71941 aaaaattatc gatcctacga caatcgaatg aaataaatgt ccaaatcacc gtgaaagaat
    72001 tagtagaatt tggacgattt ccttatacga aaggaaaatt gacgacaaaa gacgaggaaa
    72061 tcgttcagga atcactggaa caccttgggt tagaagaaat agcggatcaa tcgattgaca
    72121 ctctttcagg cggtcaactc caacgtgcgt atattgcaat ggtgctagcg caagacacgg
    72181 attatatttt actggatgaa cctttgaaca atctagatat gaattttgcg gttcaaatca
    72241 tgcagatttt aaaaaattta gtacaagaaa tcgggaaaac aattgttatt gttcttcatg
    72301 atatcaactt tgctgcaagt tatgcagacg aaattgttgc aatgaaagat ggacagttat
    72361 ttacgcaagg aacgacccaa gaaatgataa aaaaagatgt attggatgaa ttatacgaaa
    72421 tggacatacg gatttgtgag attgaaggcc gcagattctg tctttatttc taaaactaac
    72481 attaggagag aagagatgaa aaaattagta gtaggtatct tgattctagc atcatttggt
    72541 ttagcagcat gtggaaatag caacaacacc agtcaggcag acacaaaaaa aagcagcaca
    72601 caaactgaaa ctacattgac gataacagat agtaatggag accaaatcga ggtgccaaat
    72661 aatccagaaa aagtcgtcgt attcgacaat ggttctttag atacgatgga tgctcttggt
    72721 gttggagata aggtagttgg agcagctaca agcagccttc ctgagtatct ttcatcctat
    72781 aaaaaagtag aatcagcagg tggaataaaa gaaccagact tagaaaaaat taatcagctt
    72841 cagccagatc taatcatcat ttctggccga caaagagatt ttcaaaaaga tctaagtcaa
    72901 attgctccga ccattttcct ttctttagat gcgaaaaatc catgggaatc tttccagcaa
    72961 aatgtcactg ctttagggga gatctttggt aagcaagaag aagctaaaac tcaattagag
    73021 gaattatcta gtgcaatcga tcagacgaaa aagaaagcag aagctactga caaaaaagca
    73081 ctagtcacac ttgtgaatga agggcaatta tcagcctacg gatctggttc tagattcggt
    73141 ttcatccatg atttatttgg ctttgaacaa gcagatgatc aaatcgaagc atccactcac
    73201 ggtcaaagtg tttcttatga atatgtgctg gaaaaaaatc cggatatctt atttgtagta
    73261 gacagaacaa aagctattgg tggagacgac agcaaagatg atatttctgc caatgaactg
    73321 gtagcacaaa cgaatgctgg taaaaatcag cagatcatct cccttgaacc agatgtctgg
    73381 tatttaagcg ggggcggttt agaatcaatg aaactgatga tcgaagatgt gaatcaagca
    73441 tttaaatagc ataaataaca gaggattaaa ctctcatact atgtaacaat aatgtttctt
    73501 acattcttaa atagcaagag cctatcctca aagaagttat caaaaaccat ccgtaaaatc
    73561 gtattgatga ttttacggat ggtttttatt tatttgagaa atcttttttc aacgtgaatt
    73621 cattatctac atcatacggg ttgattctag aatggggagt ggcaaaaaat agtgtgatcc
    73681 attttttcag catcaagatc gcctcctttg atttgaatca aaggtacaaa gcgaaactta
    73741 gataaaactt aaatttagat atttttattt tgtaccgatc tcttctttaa acgatgttcc
    73801 atctgctgct tctcggcaat aagtccacta gactagaaaa tatgagaagc tattttctgt
    73861 ccagcgttct tattactcaa agcaaatcgc agttgtcaca atctcttcta aacaatgttc
    73921 aatcagctga ccttcggtat tagctcaaag cactgatttc acaacctcta tttaggcatg
    73981 tgtctcagca agcggttgat agttgatgat ctgcattttt cgataaaaat cggcgggaag
    74041 cttactccac cgttttaata attgatagaa ataactctct tctgctttga ttttatactc
    74101 gcttaatatc aatccaaaac tattcgtttt ttttgaagct aattttttat aaatatccaa
    74161 tgctttggtt ttatcttcat tgacaaagag tgtttttact tcaatataag aaccctcgat
    74221 attttgtgtt tcttcgataa aatagatgtt ttttatatat tccttcatag tgattcctcc
    74281 aaagaagtct tttatttgat aagtttactc taccgaagag ttgttaataa tccgtgaaca
    74341 aaaagtaatt ctttttccaa tagttgttaa aggtttttta ttttttatag tattttttgt
    74401 tatttaagaa aggaataagg ttaaacaaat aacaggttgc tttttgggtc tttttccgtt
    74461 caagaaactg aacgaagaaa agggaaagtt gtctcttttg tgataagaga aatcataata
    74521 aacgaatgac ttactttcca ataaattgag aacagtcaga accagacaaa ttagttgcat
    74581 ctgatgaata aagtgattat tctatgacat gaggtgatac agatgaaatt agaagtgaca
    74641 aaagaagcac aagaagtatt gaagaataaa ttagaagcag acgatcaact tttattagat
    74701 atcgaaaacg gtgacggtcc atttgcagaa agccaagtca gttgccaatt ggatacggca
    74761 tttcgattga tcattgtcaa aaaagataca caaacagatc taagtcttta ttctgtagtg
    74821 gctgatacac cagttggacc aatcaaaatc aaagacagtg caatacttta catggatgat
    74881 ccatccacac ttgctttaga accaacttat aatagtttac aattaaaagg accaagcggc
    74941 ttgagaaaag gcaacttgca agttgttcga aaagactaga gggataaaat gggaaaattg
    75001 gcgatcatca gcgacctaca tgtggacatc aataaattcg gcgaatcaga gctgatgcag
    75061 ctttggcaag ttttgcaaaa gcacaagatc actcggcttc atctagccgg agatacggct
    75121 aataaagtag accgttgcct agctgttgtc gaatttttcg tcaataaagg attatctacg
    75181 acatttcatt tcggcaatca cgagttggca gatgtgccca atgaacagat gatggaccat
    75241 tttccagacc cacatttttt aaatgaacgg tatattgctt taaatgagca gaccgttctt
    75301 ttagggatga atggctggta cgactatcag ttttccgaat tgaaagatta cgaggcaatc
    75361 gtacgaatga agcgtttgta ttggtatgat cggttcatcc agcgtgggaa aacagatctc
    75421 gaggtcaatg cagaaatgct tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa
    75481 aaacttgagg tgattttatc cactcatttc gttcctaaac gggcatttat catgtatcaa
    75541 tctgctcctt atgagcgttg gaacaaatta aatgcatttt taggatcaga atcattcgga
    75601 gaattacttg accgttattc aaatatcaaa caagtagttt ttggccatac gcatcgtcgc
    75661 tttgaagata aatccatcgg tcacacgatt tacagctgca gaccttttgg ttattattat
    75721 gaatggcagc tgacccgaga ttttgtcttg aaagaacagt tgctagacag ctatgatcct
    75781 atgaaattac gtgtcctctt gcgtgcccat cacccagctt ttcaggccta tcaaacacaa
    75841 catttgcaag aggaatttga acaagcgatg acaatcgttc cgtattgaga cagcattagt
    75901 aatgtgtaaa agaaaatacc ttgtaaaaca taaggataag taagagcagt agatccattt
    75961 atcgtggata tgctgctttt tttgcggaaa ataatcagcg aatgtttatt tgatcggatg
    76021 atttgtaact cgcagagtac tagatgcaag cgtgtattct ttttttgaac gataatcaca
    76081 aaaaaaaata
//
LOCUS       DACDYM010000004        61717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDYM010000004 DACDYM010000000
VERSION     DACDYM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.004"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(57..185)
                     /locus_tag="IWM59_000305"
     CDS             complement(57..185)
                     /locus_tag="IWM59_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9978370.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(188..1561)
                     /gene="rlmD"
                     /locus_tag="IWM59_000306"
     CDS             complement(188..1561)
                     /gene="rlmD"
                     /locus_tag="IWM59_000306"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9978371.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1672..2727)
                     /locus_tag="IWM59_000307"
     CDS             complement(1672..2727)
                     /locus_tag="IWM59_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9978372.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2737..4167)
                     /gene="gatB"
                     /locus_tag="IWM59_000308"
     CDS             complement(2737..4167)
                     /gene="gatB"
                     /locus_tag="IWM59_000308"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9978373.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4164..5636)
                     /gene="gatA"
                     /locus_tag="IWM59_000309"
     CDS             complement(4164..5636)
                     /gene="gatA"
                     /locus_tag="IWM59_000309"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9978374.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5636..5941)
                     /gene="gatC"
                     /locus_tag="IWM59_000310"
     CDS             complement(5636..5941)
                     /gene="gatC"
                     /locus_tag="IWM59_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9978375.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6047..8083)
                     /gene="ligA"
                     /locus_tag="IWM59_000311"
     CDS             complement(6047..8083)
                     /gene="ligA"
                     /locus_tag="IWM59_000311"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9978376.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8100..10334)
                     /gene="pcrA"
                     /locus_tag="IWM59_000312"
     CDS             complement(8100..10334)
                     /gene="pcrA"
                     /locus_tag="IWM59_000312"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9978377.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10489..12048
                     /locus_tag="IWM59_000313"
     CDS             10489..12048
                     /locus_tag="IWM59_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9978378.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12159..14090)
                     /locus_tag="IWM59_000314"
     CDS             complement(12159..14090)
                     /locus_tag="IWM59_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9978379.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14108..15022)
                     /gene="pfkB"
                     /locus_tag="IWM59_000315"
     CDS             complement(14108..15022)
                     /gene="pfkB"
                     /locus_tag="IWM59_000315"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9978380.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15019..15771)
                     /locus_tag="IWM59_000316"
     CDS             complement(15019..15771)
                     /locus_tag="IWM59_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9978381.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15930..16139)
                     /locus_tag="IWM59_000317"
     CDS             complement(15930..16139)
                     /locus_tag="IWM59_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978382.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16150..17478)
                     /locus_tag="IWM59_000318"
     CDS             complement(16150..17478)
                     /locus_tag="IWM59_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978383.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17843..18430)
                     /locus_tag="IWM59_000319"
     CDS             complement(17843..18430)
                     /locus_tag="IWM59_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9978384.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18513..19763)
                     /gene="clpX"
                     /locus_tag="IWM59_000320"
     CDS             complement(18513..19763)
                     /gene="clpX"
                     /locus_tag="IWM59_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9978385.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20010..21299)
                     /locus_tag="IWM59_000321"
     CDS             complement(20010..21299)
                     /locus_tag="IWM59_000321"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9978386.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21453..22385)
                     /locus_tag="IWM59_000322"
     CDS             complement(21453..22385)
                     /locus_tag="IWM59_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978387.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22542..23201)
                     /locus_tag="IWM59_000323"
     CDS             complement(22542..23201)
                     /locus_tag="IWM59_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9978388.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23204..24229)
                     /gene="sppA"
                     /locus_tag="IWM59_000324"
     CDS             complement(23204..24229)
                     /gene="sppA"
                     /locus_tag="IWM59_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9978389.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24242..24532)
                     /locus_tag="IWM59_000325"
     CDS             complement(24242..24532)
                     /locus_tag="IWM59_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978390.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24942..26747
                     /gene="glmS"
                     /locus_tag="IWM59_000326"
     CDS             24942..26747
                     /gene="glmS"
                     /locus_tag="IWM59_000326"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9978391.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26819..28174)
                     /locus_tag="IWM59_000327"
     CDS             complement(26819..28174)
                     /locus_tag="IWM59_000327"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9978392.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28198..29367)
                     /locus_tag="IWM59_000328"
     CDS             complement(28198..29367)
                     /locus_tag="IWM59_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978393.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29364..30251)
                     /locus_tag="IWM59_000329"
     CDS             complement(29364..30251)
                     /locus_tag="IWM59_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9978394.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30440..34120)
                     /gene="nifJ"
                     /locus_tag="IWM59_000330"
     CDS             complement(30440..34120)
                     /gene="nifJ"
                     /locus_tag="IWM59_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9978395.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34318..34839
                     /locus_tag="IWM59_000331"
     CDS             34318..34839
                     /locus_tag="IWM59_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9978396.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35538..36103)
                     /locus_tag="IWM59_000332"
                     /pseudo
     CDS             complement(35538..36103)
                     /locus_tag="IWM59_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36282..36938)
                     /locus_tag="IWM59_000333"
     CDS             complement(36282..36938)
                     /locus_tag="IWM59_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978397.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37506..38441)
                     /gene="whiA"
                     /locus_tag="IWM59_000334"
     CDS             complement(37506..38441)
                     /gene="whiA"
                     /locus_tag="IWM59_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9978398.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38475..39473)
                     /locus_tag="IWM59_000335"
     CDS             complement(38475..39473)
                     /locus_tag="IWM59_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9978399.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39470..40354)
                     /gene="rapZ"
                     /locus_tag="IWM59_000336"
     CDS             complement(39470..40354)
                     /gene="rapZ"
                     /locus_tag="IWM59_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9978400.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40489..41220)
                     /locus_tag="IWM59_000337"
     CDS             complement(40489..41220)
                     /locus_tag="IWM59_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9978401.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41224..42489)
                     /locus_tag="IWM59_000338"
     CDS             complement(41224..42489)
                     /locus_tag="IWM59_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9978402.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42752..45568)
                     /gene="uvrA"
                     /locus_tag="IWM59_000339"
     CDS             complement(42752..45568)
                     /gene="uvrA"
                     /locus_tag="IWM59_000339"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9978403.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45578..47572)
                     /gene="uvrB"
                     /locus_tag="IWM59_000340"
     CDS             complement(45578..47572)
                     /gene="uvrB"
                     /locus_tag="IWM59_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9978404.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47831..49333
                     /locus_tag="IWM59_000341"
     CDS             47831..49333
                     /locus_tag="IWM59_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9978405.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49335..50072
                     /locus_tag="IWM59_000342"
     CDS             49335..50072
                     /locus_tag="IWM59_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9978406.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50440..51606)
                     /gene="dnaJ"
                     /locus_tag="IWM59_000343"
     CDS             complement(50440..51606)
                     /gene="dnaJ"
                     /locus_tag="IWM59_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9978407.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51757..53586)
                     /gene="dnaK"
                     /locus_tag="IWM59_000344"
     CDS             complement(51757..53586)
                     /gene="dnaK"
                     /locus_tag="IWM59_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9978408.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53637..54200)
                     /gene="grpE"
                     /locus_tag="IWM59_000345"
     CDS             complement(53637..54200)
                     /gene="grpE"
                     /locus_tag="IWM59_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9978409.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54294..55337)
                     /gene="hrcA"
                     /locus_tag="IWM59_000346"
     CDS             complement(54294..55337)
                     /gene="hrcA"
                     /locus_tag="IWM59_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9978410.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPIILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55572..56738)
                     /locus_tag="IWM59_000347"
     CDS             complement(55572..56738)
                     /locus_tag="IWM59_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9978411.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56754..56981)
                     /locus_tag="IWM59_000348"
     CDS             complement(56754..56981)
                     /locus_tag="IWM59_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9978412.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57368..57624)
                     /locus_tag="IWM59_000349"
                     /pseudo
     CDS             complement(57368..57624)
                     /locus_tag="IWM59_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57967..58990)
                     /locus_tag="IWM59_000350"
                     /pseudo
     CDS             complement(57967..58990)
                     /locus_tag="IWM59_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(59628..60302)
                     /locus_tag="IWM59_000351"
                     /pseudo
     CDS             complement(59628..60302)
                     /locus_tag="IWM59_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            60825..61415
                     /locus_tag="IWM59_000352"
     CDS             60825..61415
                     /locus_tag="IWM59_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9978413.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT        17122 a        13252 c        10689 g        20654 t
ORIGIN      
        1 aaaagactaa gaaacaataa caaaagttaa cacttcttag tctttcttta gcacgtctat
       61 ttattaaaga ttttagggcc ttttctcatg tttttacgat cattcttagg tgtttccatt
      121 tgtttggatc ggcctccacc ttgtttcttt ttgatcaatt ctttcatttt ttctttttgg
      181 tccattttta ttctccttta acgaatgaag cgactaattc gatatgatgg gtttgcggga
      241 aaagatctac aggctgaact ttcttcacgg cataacctgc ttctgcaaat aacttagcat
      301 ctcttgcaaa agttgccgga ttacatgaga tatagacgat tcgttctggt gacatttcga
      361 ttgctgattc aataaaatga tgatctaatc cttttcgtgg agggtcaact acgatcacag
      421 atggtttgat ccctttttct tgccattttt tcataacttt ttctgctttt ccgacttcat
      481 aatgaacatt ttcgatttta ttatttaaag cattgaactg tgcatcttca attgcttctg
      541 gaataacttc catcccatac accttatcca ctctgtctgc taaagacaag ccaatcgtac
      601 ctattcctga ataagcgtcg atcacaatgt cttttttatc taactcggca aactcgatgg
      661 ctgtttggta cagatactct gcttgttgag tatttacttg ataaaatgac tttgctgaga
      721 ttcgataggt ttttcctaat agctgatctt cgatatagtc tctgccatat aaaacttttt
      781 ctttgtctcc taaaatcaca tttgtttttt cttcattgat attttggatg accgaaacca
      841 cttctggcaa ttcttcatga atagcttcag caatttcctt ggatttaaag aatttttctt
      901 tacgagtaac taaaacgacc atcatttcat gggaataatg accacgacgg ataatgatgt
      961 gacgaagctg tccttcattt ttttcttcat tatatcctcg tacttggaac cgttgcaaga
     1021 tatctctgac tactttgatt gcttgatcga tgacaggatc ttggatataa aaatcttcga
     1081 tagggactag atcatggcta ttctttttgt aaaaaccggt ggctaactgt ccatcgatac
     1141 ggcgaacagg gatctgtgct ttattacggt agccgaatgg ttctgccatt ccgaccgttg
     1201 gaagtacagg aacttctggc atcttagcaa tcttcgccat tacatgctct acttgttttt
     1261 gtttgaagac aagctgttgt tcatacttca aatgggctaa aggtgcgata cctatgcgaa
     1321 gtaatgcatt gttttctaca ggctgacgat caggtgattg tgtaataaat gtttcgactt
     1381 ttccatatcc aaacttattg cctgctttta ctactagtat ttctacttct tctccaggga
     1441 gtgcattttc gataaataat ggataaccat cgatttttgc tacccccatt ccttcataac
     1501 tcaagtcgac gatttcgact gtatatcgtt cgtttttctt tactatttgc tgttctgtca
     1561 tattttgctc ctttaagcct atttttcaat cctactgaac tctactataa atcagctaat
     1621 tacacaacta aaaccggcac tgaccttact acgttcagca ccggcttttg attaattctt
     1681 ttgattaggt gtttcgtctg ctatttttcc gtcaccgtcg atatcttcat ccgttaaacg
     1741 ttcgacttct tttacgaatt ctttgctgac aacctctaat tcttcttcat ctgtacctgt
     1801 gattgcttca tcaggaatct gatcggtatt ggcaaagaac tcaatgtgtt gatgaagatt
     1861 ttggaaatgc atcggtgctt ccccgccgta ttcaccatct aagttgatca ttactggtcg
     1921 attgatatca tcttctgttt caacgaacaa actgcttgtt ttggtataga ttattcttgg
     1981 atcatcgaca tgtcgaccgc cattcagcat gagtgctgca atatgcaaga tctcaaagat
     2041 atttgctgtc ttcacaatga ttaaagaaaa cttcccatca tccaatttgg catctggcgc
     2101 gatctgttcg aatcctccaa cagagttcgt cagccctagg aaaaacatcg aagcatttcc
     2161 gatatattcc ccttcatcat acttcaagcg cattttgatt ggctttactc gagggagcat
     2221 ctctgctcct ttcgcaagat aagcaagata accaaaaata ctctttagtt ctgatggaac
     2281 ttcataagtc aattctgtca aatagccacc tgcagcgata ttgatgaagt aactgttatc
     2341 agaaaggccg atgtccattt ttacggtttg gtgttttttt atcacttctg ctgctgcttt
     2401 aatattatct ctaggaattt ttaatgctcg agcatagtcg ttcgttgtac cagctgggat
     2461 gatggccatt ttaggacggt gttcaagcgg agcgattcca ttaaccactt cgttgattgt
     2521 gccatcgcca ccagctgcca ctactagatc aaaacctagt tctgcgactc tacatgcttc
     2581 attcttggct gaatctggtt ctggtgtagt tgcataggcg ctggcttcaa atcctgcttc
     2641 ttctaatgcc tgtaggatat ccgccaaatt ttttttcaat aattcttttc ccgaggtggg
     2701 gttataaatt acgcgtgctt ttttcatttt cagcctctat cgtttcgcta attcatcttt
     2761 aagtagctta ttcacgacac caggatttgc ttgtccttta gttgctttca tgatttgacc
     2821 gactaggaag ccaaccgcac gatcttttcc atttttgaaa tcatcgacag attgttggtt
     2881 gttatccaaa atttcattaa tgattggcag tagtttgttc ggatcagata attgcaccca
     2941 accatttttt tcaactactt cttgggcttc tccgccattt gtgatcaatt cacggaagac
     3001 ttttttggcg atttttgagc tgatcgttcc atcggcgatc aaacgaatca ttcctgctaa
     3061 gttctcaggt gtcaattttg tctcagctaa ttccagtttc tcactgttca agtaagcaga
     3121 aacttcaccc atcaaccagt tagatgcttg cttggcatct gcaccatttt ccaaaacagc
     3181 ttcaaaaaaa tcagacattt cttttgtaag tgtcaatacc attgcgtcat actctggaag
     3241 tccaaactca cggatatagc gttcgcgacg agaagctggc atctcaggca aactagcttt
     3301 tactttttga atccattcgt catcgatcac gaatttcggg atatcaggct ctgggaagta
     3361 acgataatca ctagaccctt ctttgacacg catcaaaact gttttattcg ttgtttcgtc
     3421 aaaacggcgt gtttcttgtt ggatttctcc tcctgaaaga agaacttttg cttgacgttt
     3481 ttcttcaaaa gctaaacctt ttttcacgaa agataaagag ttcaagtttt tcaattctgc
     3541 tttcgtaccg aactcttctt gtccataagg gcgaagagaa atattggcat cacagcgcat
     3601 agagccttct tccattttca catcgctgac ttctgtaaat tggataattg atcgcaatgc
     3661 ttctagataa gcgtatgctt cttctggtga acgcatatca gcttcagaaa cgatttcgat
     3721 caaaggcgtg ccttgtcggt tcaaatcaac gtaagaataa ccgccaatac catgcatgtt
     3781 ttttccagca tcttcttcta aatggacacg ttcgatccgg atttttttct ttttaccttc
     3841 tacttcgatt tcgatccatc cgtcatgacc gattggttga tcaaattggg aaatttgata
     3901 tgcttttgga ttatctgggt aaaagtaatt tttccggtca aaatgagtat ctttagagat
     3961 ttcacagttt aatgccaaag ctgctttcat cccgaattcc agcgcagctt tattcattac
     4021 tggcagcacg cctggataac cccagtcgat cacgtttgta ttgctgtttg gttctgcacc
     4081 aaaatgagct ggcgctggag agaaaatttt tgagttggtt tttaactcga cgtggacttc
     4141 tagtccaatg actgtttcaa aattcattcg tctttccctc ctaaaatagc tggttgttgt
     4201 ttatggaatt ctgttgcttg ttcaaacgca tatgccgctt gatacatcgt tttttcatca
     4261 aaataattac cgatgatctg caatccaact ggtaatcctt ctgataaacc tgccggtaca
     4321 gacatacctg gaagtccagc caagttgaca gggatcgtta agatgtcact catgtacatt
     4381 gtgattggat cgttaatgtt ctcaccgatt ccgaatgcaa ctgttggtga agatggcccg
     4441 atgatcaagt cataatctgc aaagactttt tcaaaatctc gtttgatcaa tgtacggact
     4501 tgtcccgctt ttttgaagta agcgtcataa taaccagcac ttaaggagaa tgtacctaac
     4561 atgatacggc gttttacttc ttcaccgaat ccttctgaac gtgagttgac atatacatca
     4621 tctaatgatt tcacattttc tgagcgataa ccgtagcgga taccatcgaa tcgttgcaag
     4681 ttggaacttg cttctgatga ggcaatgata taataaacag ccacaccgta ttttgaatga
     4741 ggcaagctga cttcttccac aacagcacct aatgaacgga atgtttctgc tgctttcaat
     4801 acagcttctt ttacgccagg ggccacacct tcgcctaagt attctttagg taaagcaatc
     4861 ttcattcctt tgatgtcgcc tgtcagtccc tctgcaaaat caggaactga aacgcctgaa
     4921 gaagtaccgt ctttttcgtc ataaccgctg atagctgtca atgctaatgc attatctttc
     4981 accgtacggg tcattggtcc gatttggtca agagatgaag caaaggcaat caaaccaaaa
     5041 cgtgagacac ggccataagt aggtttcatc cctacgatgc cgttaaaagc tgctggttgg
     5101 cggatacttc ccccagtatc actacctaaa gaaactggaa tttgtccagc tgcaactgct
     5161 gctgcagaac caccagaaga gccaccaggt acttttgttg ggtcccaagc attttttgtt
     5221 ttcttgaaat aagaagtttc tgtacttcca cccatagcaa attcatccat gtttagctta
     5281 ccaacaggga tcatatctgc ttgatagatt ttatccatga ccgttgcatc ataaattggt
     5341 tcaaaattgt aaaggatctt agatgcagcg gttgtcaaga tatcttttgt tacgatatta
     5401 tccttgatac caattggaat accagcaagg acgttgcttt catcaatccc tttttcatct
     5461 aaagcttttg cctgttccaa tgctttttct tcgttcaatg taatgaatgc ttcgacctca
     5521 ggctctgttt ctttgattcg ttcaaatgtt gcttttgtca aatcttgtac agtgatttct
     5581 ttcgatacaa gcatatgatg cagttcttct agagaacgat tgtgtaattc tgtcattatg
     5641 cgccagcctc cccattatcg atgattgcgg gtactttgat aaagccgtct tctgtttccg
     5701 gcacattttt caataattca tcgcggttcc atccagcttg cgcccgatct tgtcgcatca
     5761 cgtttactgt ttcaaccaca ttcgacgtaa atggaacacc ttctgtatct acttcttcca
     5821 gcatttcaac catatcgatg attttaccta attggtcggt aaaacttgca agttcttcat
     5881 ctgcgaacgc aagttttgct aatttggcaa cgtgtttcac ttgttcttca ctaatcgcca
     5941 tactacccct tctttctaaa cgagttaaaa actttgctct ctattttaac acaagagtgc
     6001 cgtgtctgaa attgtttatt gaaatttgtc tgaaaaaact catttttcat tcttctactg
     6061 agcgactttt agccaatgca tctgccattt gatcttctgt ccaaacttcg attcctaatt
     6121 cttgtgcttt ggtcagcttg ctgccagcat cttctcctgc taccacgata tccgtttttt
     6181 tcgatacgct accagtcact ttcccgccaa gattttcgat cgtctctttt gcttcttcac
     6241 gagtaaatcg tgttagttta ccagtaagga ctactgtttt ttctttaaaa ggagactcga
     6301 cttcttgcaa ttgggcattt cgaagccctt tgtattcgaa attgacgcct gcttgtttta
     6361 attcattcat caattcatgg acttcttcgt tactgaaata agtgactaca ctgtcggcaa
     6421 tgatatcacc gatcgtatca agcgcaataa tcgactcata gtcactttta ctcaatgttt
     6481 ctagatcacc gaaatgttct gctaaaattt ttgctgcttt cgctccgaca tgtcggattc
     6541 ctaacccgaa aatcaaccgc tcgaccgaat tatctttgct gttgtcgatt gctgtcaaaa
     6601 tattatttgc tgatttttct tttattttgt ccaacgtcaa taactcttct tctgtcaatt
     6661 tgtaaagatc agcaacatct gctactagtt ttttatcata catctgttct agtacgcgcg
     6721 gccccaaccc atcgatattc atcgcatttc ttgaaacaaa gtggttcaaa ccttctttca
     6781 tttgagcagg gcatttagga ttgatgcacc gtaaggctac ttcttcatcc aaatggacaa
     6841 gttcacttcc acaaactgga caatgtgtag gcaattggta ttcttcgctg tctttaggtc
     6901 gtttatctaa cacgacttgc gaaacttctg ggatgatgtc tcccgcttta taaatcagaa
     6961 ctgtatcttt caagcggata tccttcaact taatataatc accgttatgc aggctggcac
     7021 ggcttactgt cgttcctgct actcggactg gtgtcatgat agctgttggc gttactaccc
     7081 ctgttcgacc gactgtccat tcgatgtttt cgattagcgt ttcaacttct tctggcggaa
     7141 acttgtaagc tgctgcccat ctcggtgctt tgacagtaaa accaagttga tcttgtagcg
     7201 agaattcatt cactttgatg acgattccat cgatttcata gggcaaatcc acacgtttgt
     7261 catgatattc ttcaatgtat gaccacactt catcaatcga atgacataga cgtttttccc
     7321 gattcgtgtg gaatccaatt ttttctagct cttctagcgc atcatactgg gtttttgctt
     7381 gcataggacc gaaatcagct actgtgtata aaaaagtgtc cagatttctt ttcgctgtga
     7441 ttttagaatc taactgacgc aaagaaccag ctgcggcgtt ccgaggattc gcaaagatat
     7501 cttttccctc tgcttctctg tcttggttca attggacaaa tgaccgtttt ggcatgaaac
     7561 attcgcctct tacttcgatg ttcattggtt cttttaattt gattgggacc gaccggacag
     7621 tttttaggtt ttcagtgatg ttttctccta cagttccatc cccgcgagtc gcacctctca
     7681 cgaaatttcc atcttcataa cgcaaagaaa cggaaagtcc atctattttt agttcacagc
     7741 aatagtcaac tacacgtccc actgcttttt gtacacgttg atcaaaagct atcagctctt
     7801 ctttgctgaa aacgtcgttc aaactgtaaa gcggaatatc atgagtgact ttttcaaaac
     7861 cttctaatac ttttccgcct actcgttgag tgggagaatc agacgtgatc aaatcaggat
     7921 attgttcctc aattgctact aattctgcgt attctttatc ataaacatag tcttccactg
     7981 ttggtttatc ttctacgtag tattcgcgag accattggtt cagtcttgta cgaagttctt
     8041 ctgctcgttt agcagcttcg tcaatcgtca tttcatgttc catcatttac cctccactac
     8101 taagcttttt caattggcgc aaaggcagcc aaaagacgtt ttactccttg acttgggaaa
     8161 gccacatcta gttctagatc cttcgacgtc ccgcttactc ggacgactgt tcctagaccc
     8221 cattttttgt gtttgacttt atctccaggt ttccagctgt tattttcccc tccagtagca
     8281 cttttagagg gaacaaccgc tgtctctggc tgtttatagc tatatgctgt ttttttagcg
     8341 aatacaggtg tttttttagg cgtaggacgc atgccttcga tttccagtaa ctcctgatcg
     8401 atttcttcta caaaacgaga aggtcggtta tattgcgtcc gtccatataa tgttctggag
     8461 aaagcatttg ttaaatacaa cgcttcttct gctcgcgtga tccccacata tgcgagacgg
     8521 cgttcttctt ccagttcgct ttcttccatc aatgctcgag ataaaggaaa aaccccctct
     8581 tctagaccaa tcaggaatac aactgggaat tctaatcctt tggctgcatg caaagtcatc
     8641 aatgtcactt gggaagcatc ttcttctaaa ttgtcaatat cggaaacaag tgccagatca
     8701 tttaagaaga ccgttagttt ttcttctggt gcatcagcgt cttcttcatt ttgctgttcg
     8761 aattgtttgt cgaattcttg tgtgaccgtt aaaaattcct ccaagttttc gatgcgtgct
     8821 tgtgcttcta atgtattttg gatttttaag tcttctagat atccgctacg atccaaaacc
     8881 tctttcgtca attctgtcac tgtcaagtat gggatcatct gagtgacttc ttggatcatc
     8941 tctccaaatg cacctaattg ctgtcctgct tttccaccga tatttgccag atccacattt
     9001 tgcgctgctt caagcagtgg ccattcatga agagaagcaa aactgcgtag tttttcgata
     9061 gatcctggtc cgatgcctcg tttgggcgaa ttaacgatac gctcaaaact gattgaatct
     9121 tgaggatttg ccagcacatt caaatacgct aaaatatctt tgatttcttt acgatcgtag
     9181 aacttgtgcc ctccaaccat tttataagga atatttgctt tcaacagagt ttcttccatt
     9241 acacgagatt gtgcgttcgt ccggtacaag atggcaaagt cgccgtaatt ccgatgattg
     9301 cttcgaatct cttcttgcat ccgatctacg ataaaacggg tttcgtctcg ttcattatct
     9361 gcacggtagt aagtgatctt gtttccttct ttgttttctg tccacaagtt ttttggtttt
     9421 cgattgctat tgttttcaat gacttggttt gctgcgctaa ggatgttttt cgtggagcga
     9481 tagttttgtt ctagcaaaat tactgcagca tctggataat ctttttcaaa atccaagatg
     9541 ttctgcatat ctgcaccgcg ccagccgtaa atactttgat ctgcatctcc cacgacgcac
     9601 aggttcctaa atctacctgc caataaattg actagcgtat actgtgcgtg atttgtatct
     9661 tggtactcat ctacatgaat ataatggaat ttgttttgat aatatgtgag tgaatcagga
     9721 tgttcttcaa ataatcgaat cgtattcatg atcaggtcgt caaagtccat acattgatta
     9781 tattgaagct ctttttggta agcagcataa cattttgccg caatctcttc gtaataactg
     9841 ccttgcattt ttgcaaaatc ctgaggggtc tgcaggctgt tttttgcttg agagattgtt
     9901 cctaatatac ttcgaggatc atattttttc ggatcaatgt tcagttcttt tagtatccgt
     9961 ttcatcaagg tcagctgttc agaagaatcg atgattgtaa agttccggtt atacccgata
    10021 aaatcaacat ctcttcttaa aatacgcaca cacatcgagt gaaaagtcga tacccagaca
    10081 tcttcgcctc cagatgcgag aatcgcattg acacgttctt tcatttcttt tgcagcctta
    10141 ttggtaaaag taatcgctag gatgttccaa ggattgactt ctttttcctc aatcaaatag
    10201 gcaatgcggt gtgtcagcac tctcgtcttt ccacttcctg cacctgccat tagcagtaat
    10261 ggtccgtcag tgtgaagaac agcttctttc tgacgggggt tcatcccgtt taataattca
    10321 gctttggatg acattaacaa catcctttct tttatcaatc ttgttaatta tagcattttc
    10381 ttggctttca ttaaactagc tagttcaaaa tcatttgttt ctttttatgg atttttcctt
    10441 gtcaattcgt tgatttcgcg ctaaattaaa agaaagcgag gcgtttgaat ggaaaaaaaa
    10501 ggaaaaatca agaaattaga taagactcag atatatttta ttctaaaagg aatatttgtt
    10561 ggactgctga caggaatcgt cgttagcctg tttcgattat ctatcgaaaa gctaagcgat
    10621 ttagttcgaa gtatctatga aatgagtcgg gaacagccga tttacttaat tggcctagct
    10681 atttgctgca tccttgcagc atttttcgtg ggttatctgg tcaaaaatga accggatatc
    10741 aagggaagcg ggattccaca agtagaggga cagcttcgcg gggaattatc aatgaactgg
    10801 ttctctgttt tatggaaaaa gttcattggc ggtgtacttt cagttggtgc gggccttttt
    10861 ctaggtagag aaggaccttc tatacagctt ggcgcatcca ttggacaagg agctggacaa
    10921 ttgtttcgga gtccatcttc agaagaaaag attttaattt ctagcggtgc aagtagcgga
    10981 cttgcagcag cattcaatgc tcctatcgca ggattactct ttgttttaga agaaatccat
    11041 cactcctttt ctccacttgt ttggctgaca tcatttgctt cagcaatcac tgctaacttc
    11101 gtctctcttt actttttcgg cttgagacct gttttatatc taggtgacct tccttctttg
    11161 ccgttgaaat attatggttc cttagtgatc ttaggagtac ttctaggcat cttaggtttt
    11221 atttatcaga aggtcttgct ggctttgcca aaattttaca accgattgcc tttaccagct
    11281 tattttcacg ggttcgttcc tttcttgctg attttgccaa ttggttattt atggcctagt
    11341 actctaggcg gaggcaatca aatcgtttta gcttttggac atgcttcctt gccaatcatt
    11401 atgattatcg gtttgtttgt tcttcgattc gttttttcca tgatttctta cggtggtggt
    11461 cttccagggg ggattttcct tccgatattg acattggggg ctttaatcgg tagcttttat
    11521 ggaaacatta tggttgattt cgggatggat ccaatctaca tcaaggattt tgtcgttatt
    11581 gccatggctg gttatttcac agcgattgga aaagcaccgc tgacagcaat tattttagtg
    11641 acagaaatgg ttggctctct taatcatctg atgcctttag gattggctgc tcttgttgct
    11701 tatatcgtca atgaccttct cggcggtaat ccaatttacg aatcattatt ggaacgtcta
    11761 cttggaaata ctcagaccgc ctcactaaaa ggtgcaagga caacttttga ttttccagta
    11821 acggcagaga gttctttaga cggattaatg gtacgagact tcaattggcc taaaaatatg
    11881 ctgctaatct ctatacgcag aggaaatcag gaaatcttga ctcacggtga tacgatcatg
    11941 aatgtaggag atattttggt tatcttgact gatgaagcaa acttgccaag aatcaagcag
    12001 gaaatcgagg ctaaatcttc tatcacaaat ttgaagcaat cattttaacg aacatggaaa
    12061 aactatttta caacaactat tcatctacac tacttgattc tatatgattc tataaaaaaa
    12121 gaagagactg ggaaaaacca gcctcttctt ttttatttct atgcttctgc tgctgcagga
    12181 gcttttctca ataaaccata aatcacacca gatacgactg ctccgatagc aataaacacc
    12241 aaatacatga ccgggtgact cagtaagaat acaacgaaaa tcccgccatg aggagccagt
    12301 aatttgatcc cgaaagctcc gactaatccg ccagttaaag cagaaccaat aataaaactt
    12361 ggaatggcac gaatcgggtc agctgctgca aatggaatcg atccttccgt gacaaacgat
    12421 agtcccatca cgatgttcgt caaaccagca tcttgatctg ttttgctgaa tttattctta
    12481 aacaaacgtg tagcaacgaa aattgccaat ggaggtacca ttcctccagc cataacagaa
    12541 gccatcacga cacttccgcc agttgcaaca gtcgtagcta aagttgctgt accaaacaca
    12601 taagcggctt tgttgattgg accgcctaaa tctgccgcca tcattccagc taataatgca
    12661 ccaagtaaag cagcattcga accactcata cttgtcaaga aaccgttcaa tgcatcattg
    12721 attgctttca tagggacatt gaccagcaac atcaagaaac cagtaatcag taagccaaac
    12781 actggataaa agaggatcgt tttaattcca tccaatgatt ttggtaagtt cttgaataat
    12841 ttacgtaaaa atacgatgac ataaccagct aagaatccac caatcagtgc acctaagaat
    12901 cctgcaccgc ctgtattagc taatgcacca gcagcaaatc caacgatcaa accagggcgg
    12961 tcaccgatac ttgaagcaat aaatcctgct aataccggca gcatgaaacc aaatgcagct
    13021 tgtccgattt ggttgaacca gctagcagct tgattgtaat tccctagatt tgataattga
    13081 tcatgtggca cgccaataaa ttggtcgatc atgaaagaaa gcgcaatagc gattcctcca
    13141 ccaataacga aaggtaacat gtgtgatacg ccgttcatca aatctttgta aattctttgt
    13201 ccgatagtac catccgcatt tccttcttct gaactgcttt cttttccatc accatggaat
    13261 actggtgcag taccactaag ggctagattg atcagctctt ctggtttacg gataccatca
    13321 ctgacaggac gattgatcag ttcttttccg tcaaagcgat tcatctctac ttttttgtct
    13381 gctgcaacga ttacaccttc tgcacgggcg atgtcttcag cagtcaatcg gtttttgatt
    13441 ccttctgaac cgttcgtttc gactttgatc tccacaccca tttctttcgc ttttttcttc
    13501 agagcatctt ctgccatgta agtgtgagcg atccctgttg gacaagctgt aacggcaaca
    13561 acgaattttc tttctggatg agcagaagct tctgcagctt gtgtttcttg ttctgctttt
    13621 tcttctgctt ctttttgctc ttcagctgtt tggaacaagt cttggacttc ttctggtgta
    13681 tttgcttctt ttaattttcc tacgaaatct gcatcaatca gcaatcgaga tagtgctgct
    13741 aatgcttgca aatgtgtgtc gtttgcgcct tctggagctg cgatcatgaa aaacagataa
    13801 gttggctgtc cgtctaaggc ttcataatct actccttttt ggctttttgc gaataaaact
    13861 gttgcttctt taactgcact gtttttcgcg tgaggcatgg cgattccatc accaagacct
    13921 gtagaagttt gtgcttcgcg ggctagaatt ccttctttat atgtttcgat atcggaaatc
    13981 cggccggcat caaacatttt ttggaccatt tcatcgattg cgcctttttt atcagtcgct
    14041 tgcaaatcca tgatcatcgc ttctttgacc attaaatcct tgatattcat gattatcctc
    14101 cttgaaacta atcaattttt gtgatttcaa cttctggcag caactcatca atcaattcgc
    14161 gagctgccaa gtcatcagaa aaagctgttg cgcttccgca agcaactccc catttaaagg
    14221 cttcgattgg atctttcgta cggctgtaag ccccaataaa gccggcaatc attgaatccc
    14281 ctgcacctac tgaatttttc aatgggcgaa ctaatacatt cgttttataa gttccttgtt
    14341 ccgtgaagag caaagcaccg tctccagcca ttgagatcag cacattctgt gccccttcag
    14401 ctagcaattt tttaccatac ggaatgattt cttctacaga ttggaaggtt gtattgtaaa
    14461 gctcagctaa ttcgtgattg tttggtttga tcaacaatgg tttcttaaca agtgcatttt
    14521 ttaggtcatc acctgttgta tcaatcacga aatctgcacc tttttctcga atgatatcaa
    14581 tcagttcttg ataaaatcct tttcgcaggc tagccggcgc gcttcctgac aatacgacga
    14641 tatctccttg tcctactgtt cctaatgctt gcttcaactc atcgatttct tcatctgaaa
    14701 tagatgggcc caaaccgtta atttcagttt cagtatctga ttttagtttg atattgattc
    14761 ttgtatcttc ttttacggtt gtgaacaaag actggatttg ttctttttca agccacttcg
    14821 aaataaattc gccagtgaac cctcctaaaa accctagtgc tgtagattga gaatcgatcc
    14881 gtttcaaaat acgggaaaca ttgatccctt ttcctccagg taacttgaag tcttccttca
    14941 ttcggttcaa atcgcctagt tttaacccat ctacgcgtac gatataatca attgatggat
    15001 taagtgttac tgtgtaaatc attgtattgc ctccttaatt gttgtctttt cacggaattc
    15061 attaacgaat tctaatggac aacgctcggt tatgatcgtg gctgcttcca aaggtgcaac
    15121 agaggtgaag gacaactggt tgaatttggt atgatcaatc aatacaaatg tctcttcgga
    15181 atttttcatg gctaaacgtt taacagctgc ctcttctggg tccggagtcg tgaatccttg
    15241 ttcaagatgg gcaccgttca ttcccataaa tgctttatta aaatgaaaat tttttagttg
    15301 ttctatcgca ttagatccta aaacagcatt agttgccagc tttatcgttc cacctaaaat
    15361 aatggtagca agctccaaat caactaaatt tgccgcatgt tgaacggaat ttgtcactac
    15421 tgtgatctgt tttccttgca gaaaaggaat catttctaac gtagtagagc cagcgtctaa
    15481 ataaatgaca tcttcgtcat taatggtttc agctgccgat ttggcgattt gctgtttttc
    15541 gtgaacgttt ttgagtgatt tttcactcat attctgctca aaattcaata attgctcttt
    15601 cttagctccg ccatgaactc tttctagaaa cccactattc tccagctctt gtaaatcacg
    15661 tcttatcgtt gattcagaag cattaaaaag gtttacaaga tcttgtgatt tcacgatttt
    15721 ctgctgttca agcaagtcta ggattttttg tctacgttcc tctgtaagca ttttcacgat
    15781 cacctcaata caaataatag catatttttt ttctactcgc aatataaatc agtcaaaaac
    15841 gttcataaaa attataaatc agtcaaaaaa aaataaaaaa ccagtcaaaa agactggttt
    15901 tttatttttt tgtctatcat caagccgttt tatcgttttt tattttcgct tcttgttttt
    15961 ttcatataaa cagaagcagg ttttctttcc ttatgaagaa gataataata aagtcccatc
    16021 ccgccaaaca tgaccaaaaa accgatgact gatcgaatag gaaaattagg aacaaaaaag
    16081 agacagacgg ataataatga tgccggaacc attattcgat aatagagttg tttatccatt
    16141 ttcttcacct catatttttt gtaaagtaat caattgttct aattctcggt aatctaaaaa
    16201 ttgcacacgc agatgctgct cacctttctt gacatcgaat tggatacttc gccgcttttt
    16261 ctttattcgt ttgaaggtaa catcagagag tctatgtttg atttccttta acggtaattc
    16321 tgacagggat tctgaaggaa catagattct taaggataaa agatactctt gatatttttc
    16381 atctgttacc tctacttcta gatattccct aatataataa caataagctt cgattccatt
    16441 atccaaagca acagtcggat cgataacttt atagcacatc atgcgcgagc gattctcgtc
    16501 aacccaagga aaatacgcct gagcatttag tgttccgcga acacgccttt ctttataatc
    16561 tccttgttga atatcataac tttttgttac aaaaaatgaa gcattctttt taggaacagg
    16621 ttgcatttgt gcattctgat actggacaga ttggatcgta tggtcagtgt aaatgtattc
    16681 aacgaatggc ggtttcaata aaccgacacc attgtcccaa gcaagatgat caaacccaag
    16741 ctggtcccta aaataggatt gaaacatttg attgtttggc ttttgccagt taaaggggtc
    16801 tcgataaagc tggatacata tgacttcgtt ttctaattcc ttcacttttt ctacttgttg
    16861 gatgtcgaga aaaatcggtt tagggatgat ttgatgataa tagcgactat aatacatttc
    16921 ccaacacgaa gtgaaacata aaccttggta aaaaagatcg tatcctggaa attggttgca
    16981 atcgactact acttccccgt tttgttgttt cattttaggt aaatgttcga tttcatcctt
    17041 tttctcaaac aatagccgtt gagagatctg ttttgtattg tgatagataa actcgctata
    17101 agctcgcata taggcgaaca ccccatgagc aatcattttt ttctgaatga agtctcgaat
    17161 caaaaattgc ttttctttga aaacagagat agacaataca ttttttatca ataattgatt
    17221 atcttccaag cgaaaactac attcgttctc gtcatttcct acaactaacc aactttcgtc
    17281 tcttaccgat gctagcatta ttttttcttg aagctttcga agatcatacg gatccgcttg
    17341 ttgtgtgaaa gagcggataa atgttggctg gatcaacaga cgtgtcaaaa tagcccaatc
    17401 gcttatttcc tgtttttttt cttcttcatt taagtctgtg agctggatag tcatcaataa
    17461 tttatcttcg ccaatcattt tctcacctct ttaaaatctt tttataattt aattataacc
    17521 aacgataaag aaaaactaaa actttatgtc ttagaaacca atttggacga ttacatgata
    17581 gactaagtaa aaagaaacaa agaggtgttt acaatggggt tgaaatttga acgtacagat
    17641 aatttagata aacttttcga atcatttgct gtcgatccgg aaaaaacagt tgcaccaaaa
    17701 gaagagatcg atcgatttct aaaaccacag ccacaaaatg aaaagaaaaa agacaagaaa
    17761 caaaagtaag ttaagctcaa aaaaagactt accagccaaa cttttatgaa agagtttgac
    17821 tggtaagtct tttttattct ttttatgatt caattgcttg ttcgattgct gcccacgctt
    17881 cttcttttcc ctgttttgta acggaagaaa agagaataaa cgtgtcattg cgatcaaaat
    17941 ccaacgcttt tttgatttcg ctctcatgct tgttccattt accacgaggg atcttatctg
    18001 ccttagtggc aacaacaatt acagggatat cataatactt caaaaattga tacatctgga
    18061 tatcttcttg agaaggctta tgacgcaaat ctactaaaga aacgaccgca cgtagctggt
    18121 ctctagtcgt taaatatgtc tcgatcatct ttccccattt tgctcgctca gttttggata
    18181 cttttgcata cccatatccc ggaacatcga caaaatgtaa tgcgttctcg atcaaataaa
    18241 aattcaatgt ctgggtcttt cctggcttac cggaagtacg ggctaaattc tttcgatcaa
    18301 tcaatgtgtt gataaaagaa gatttcccta catttgatcg tcctgccaga gctatttctg
    18361 gcaattgtgt atctggatat tgttttggtg ccactgcact tatgataatt tcagcttgat
    18421 ggactttcat ggtctctctc cttaacaagg tgatgaataa agcaagcggc tgtcgcaaaa
    18481 gccaaaatga cttttacggc agcctctttt catcaacccg cttttttctc tgaatcttta
    18541 tagattactg ttggctcacc agacaattca gctgcttctt ttgtgataat cactttttcg
    18601 atgttttcat ttgaagggat atcaaacatc acatccatca tgatttcttc aatgatggaa
    18661 cgaagtcctc gagcaccggt attgcgttcg attgcttttt tcgcaatggc tctcaacgct
    18721 tcaggttcga attcaagttc ggtattatct agagaaagaa gtttttgata ttgtttgact
    18781 agcgcatttt tcggctcagt caagatccgt accagatcat ctgttgtcaa tttctctaaa
    18841 gctgccataa caggtaatcg tccgataaac tcagggatca atccgaattt tagtaaatct
    18901 tcaggaatga tatgctgcat gacactttcg tcttcagaaa gtttttggtt ttgtgtacca
    18961 aatccaatcg tcttttcgcc catacgattc ttcacaatcg tttcgatgcc atcaaatgca
    19021 ccacctacga tgaacaatat gttcgtcgta tcaatctgaa taaattcttg atgtggatgt
    19081 ttacgtcctc cctgaggtgg cacgcttgca acagttcctt ctagaatttt cagcaaggct
    19141 tgttggacac cttcgccgga aacgtcacga gtaatagata cattttcgct tttacgcgca
    19201 attttatcga tctcatcaat atagatgatc cctttttctg cacgttccac attgtaatca
    19261 gcagattgaa gcaatttaag caaaatgttt tctacatctt cccccacata accagcttct
    19321 gtcaaacttg tcgcatctgc gattgcaaaa ggtacattca atgtttttgc taatgtttgt
    19381 gctaaaaatg ttttaccaga ccctgtaggt ccgatcaagc agatattgct tttttgcagt
    19441 tcaacatcat ctgtgttttc ttgtgcttgc tgattgacac gtttatagtg attgtatacg
    19501 gcaacagcta acgagcgttt tgcgcgatct tgtccaatca cgtagttatt caatacttca
    19561 agaatttcta gtggtttagg gacttcagtg aattcgcgta cagcttcttc ataaaattct
    19621 tcatcaatga tttctttaca taggtcgata cattcattac agatgtagac ccctggacca
    19681 gctacgatct ttttcacttc ttcttgggtt ttcccgcaaa aagaacagcg tacggtctca
    19741 ttgctacttg gattgtcata catggtcttc accccttaac ttgcaaaaag tatgcaagaa
    19801 actatcaaag actttacagt cttttactat cataacatag tttagtaaaa agaaaaagtg
    19861 tttaacaaag gatcaactga cctatacctt tgcatttttt actcttaaca aaaaataagg
    19921 aactgtgaca aaatcgtgtc acagtccctt atttcgaata aacaattttc tagaagtagc
    19981 ttcttcagaa atgatttatt acttgatttc tattctttcg cttctgtcgc tgtacctgtg
    20041 atcaattcga ttgctttttt cattgtcaca tcatgttcca gcatatcttc tgtcaacacg
    20101 cgtttgattt gttcgattgg catgttgtat gtttctgata attcttggat ttctgcattg
    20161 atttcttctt ctgttgcttg aagattttca gcagctgcaa ttgcttcgat cacaagattt
    20221 gttcttgtgc gcatttctgc ttcaccttcg aattgtttat gcaagtcttc ttctgtagaa
    20281 ccagttaatt gatagtacat ttcaggagag ataccttgac gttgcatgtt gttcaagaat
    20341 tcgtccattg aacgatgtac ttcatcatga accatcacgt gtggcaattc aacgatttct
    20401 gcgttttcaa cagccatacg gatcgctgct tcatcttttg catcgtccgc tgctttttct
    20461 tttgtttcta ctaattcttt gcgatatttt tctttcaatt cgtctaatgt tgatacttcg
    20521 tcatcaacat cttttgcgaa ttcgtcatct aattcaggca cttcttttgc ttttacttca
    20581 tggattgtca ctttgaagac tgcttctttt ccagcaagat cttctgcttg ataatcttct
    20641 gggaaagtta catttacatc tagactttca cctgatttat gaccaactaa ttgatcttca
    20701 aaacctggaa tgaatgagcc tgaacctaat tcaagtgagt aattttcgcc ttttccgcca
    20761 tcaaacgctt cgccatctac aaagccttcg aagtcgatta ctactgtatc gccattttca
    20821 gctgcagcat cttctttgat aactaattca gcttgtgctt cttgttcacg tttgatacgt
    20881 gcatccacgt cagcatctgt tacttcacgg tcttgttttt caacaactag atctttatat
    20941 tgtcctaatt ttacttctgg tttcactgtt acttcagcag tgattaccca gtcttgacct
    21001 ttttccatgc tttcaacatc gatttttggt tgagaaacag gatcgatgcc agcttctttg
    21061 acagcagctt cataagcttc tggtaataca gcattcaatg catcttcata taaagcttct
    21121 tcaccgtaca tacggttgaa tacttgacga gaaactttcc ctttacggaa tccaggtacg
    21181 ttcaagcttt ttttcacttt attaaacgca gttgttaatc ctttttggat ttcgtcttgc
    21241 gcaatcgtaa attttaaaac accatcattc gtgccttttt tttcaaatgt tgcagtcatt
    21301 taattccctc cgagtttaga aattcatttt atacatcaaa aacgtttcat gcataccttt
    21361 aaatagtaca ctaacccttt caaattgtaa acatattgaa tggatagaat ccttgttttt
    21421 cataacattt ttatcttttt cactgataaa atctaatgga agaagatcat ttgctgcaaa
    21481 attttttctc ttttttttaa aaaatcttgt ttttcatctt ctgtaccttc atacatcgtt
    21541 tttcctgtac acgcctctac agtcagttga aaccagcgct tcgggtcttc gatgtatgta
    21601 tcatagacag gataaacaat tgcagattgc aacgtgaatt cttcatttaa cacggaacta
    21661 agcagcgcat cttcattacc gtaacgatct gaaaatacac ataattgctg gtaacttgcc
    21721 tgctgctgcg gctttggcag atcttttggt atgactttga ctaacttttc ttcaatcgtc
    21781 aaatagaaaa caggatggca ttcgttcaat tgcctaagtg tctccagtaa ttttgccctt
    21841 gctaaaatat ggacctctgg cgatacaata agtttactcg ctgtttggat aaactcatga
    21901 taaggaagct gttcgatctc atcaatagac gcaagctgac gtgcaggttc caacgaagga
    21961 agttcctccg atttattgtg gagtgccttg atcttctgcc tctccatttg tccggataat
    22021 tcttctgcaa attggatttt ttcaatgacc cgctgctcga aagcttcata aaaatctgta
    22081 cttgctatca attttctagc atataaaaaa tcttgtgtac tgattaacaa gtcaaaataa
    22141 aattcagcaa actcctcatt ttgaagatac tgtttttcat gcaagagtgc ttgcttcaat
    22201 gcttcttttt tctcgtccaa ctcgtaaaga caaaaaacaa gcaggctatt agcctcaaag
    22261 ctttctttca actcgtaagc tcgttgaaat aattcttttg cctgctgata ttgctggttt
    22321 gctagtgctt gttgtgctac tcgcattaaa ttctcatatt tttctggaaa atcaatcggc
    22381 ttcacgcagt gtcctcctct tgctcatgtt cagctcatgt tcaatagtag caaaacaaaa
    22441 aaagcatgac aattaaaaat aactgaagca ccctctaaaa gctagatttt agatctgact
    22501 tttagaaggt gctttcaact gttactgctt tttattgctc atcagctgtt gacttcctgg
    22561 ttgaatgctt tgatcagatt gatcgtaaca acacttgtat tagtgaaaaa atcgcctaga
    22621 tgttgctttt tctgattaaa gattgctgga agatacccta acatcagcaa aggaaatttt
    22681 agaatgaacc ggcaagcacc ttctctaact aaaacggttg accagctaag ttcttcttca
    22741 tcaaagctga tgactcgaat accaaagatc attttcccaa tcgtttgacc atgattgaat
    22801 ttcgtcaata aaataaaata cgctagataa ataagtaagg ctgtgaatcc gtagatactc
    22861 agataagaat ctgaaaaatt aagccaacct agatttttgg caataccaag tgttaaacgc
    22921 gtaatagctc cgatacacaa aagatcgacc aagaaagcaa acattcggat aaaaaaacct
    22981 gcatagaaat acttaggaaa atcatttgct tttggcggtt catttcttgt gtccgtatac
    23041 tttttccatt tacgttgttt ctttttgatt tcttcgggcg tcaaaggctc tctttcaaaa
    23101 gagctcagta ccttttggga aaagttggag tcaggtccta cagcaggctg ctgttgctta
    23161 tctttttctt gttccttatt ttcatttgaa agctctgaca tgcttactca cctccgtaat
    23221 aatacattgc ttttggtgat tccgctgtcc ctagattctc aagaacggat aagatctgac
    23281 tagtctcaga aggcttcaat ccctggaatt ctgctagttt acttcctaac catgtactag
    23341 caaacccaga tggcgtggaa gaatattcta ctagttccgc atcttcaagc tgttgttcct
    23401 ttcgcatagc ttttaaagca tcttcaggaa atcccaattc atcgactaaa cctacttctt
    23461 ttgcttggac accatcataa atccttccat cagcaatttt tttgacttct tcttcagatt
    23521 tattccgtcc ctcgctgacg attgctacaa aacgactata tgcgctgtcg atatatgctt
    23581 gaagcaccgc ttcttcttct tttgtctctg gtcgcgttcc tgaacccata tctttcaacg
    23641 ctccgctttt aactgttgta tcttccacac ccagtttttc tagaagtcct gaataattca
    23701 atcctgacat gatcacacca attgagccgg tcactgtttc ttctgttgca aagattttat
    23761 ctgcttgagc agaaatatag tagccaccgc tagctgccat gtttttcatg ctgacataca
    23821 ttgggatatc atgttcttta cgaatcgtat ccaatttctt agcgatttcc gcactttcgt
    23881 agacaccgcc accaggagaa tttacttcta aaaagatacc tttgactgtt ggatcttctt
    23941 caatcgcctt cagttgttcc ataaaatttt tatgattgta accttcagaa gcaaataaac
    24001 ctgaagaccc tcccgcagca atcgttccat ctactgataa ttttacgatt ttttgcgtac
    24061 tgtctccctc ttccaacaca tttgaagcta gttcttcatc cccatataac caactgttca
    24121 atccctcaat ttgatcgttt tcctgaggct tagtcaaact agctgaaact aacgaaacca
    24181 caaataatcc tgcagcaatc agaactgctg cccaacgtct cttattcatt ctttctcctt
    24241 tctaatacaa gttttttatg acgatcacag tttctgactg ctcagaagtg tcgaaccgct
    24301 ctgctttttc ttcccgcgca tacccggaaa acgactcttt tgtcaattca tcttctaccg
    24361 taaactcacg agcttcagca acttccaagg catcaggtgc gatttcttgg atgctatccc
    24421 agccagtatc taaaaatatt ttcgtatcat ctgatacacc agctgatgct tcgaatttgg
    24481 acaattgttt ttttaacatt cccagtgtca tgatctcaat ttttgaatcc atttttactc
    24541 ctcctctatc cgattacttt ctattgtacc gaactcctga aaaagatgat agataaaaac
    24601 ccattatttt ttgacataaa aagagcattt gacaaagcat ttgatatata ccaatttatt
    24661 ttttattctt tttttgttga caagaacatt cgcaaaaggt tatgatttta aatgtttcca
    24721 gataaaaaac aaaagaggag gatagttata ccttttagga tagcgccagg tctgtgtaaa
    24781 cagatgacga ggagagggtt tatcgaaaga ttcggcggat ggccctaggg actgcactct
    24841 acagaaaatc cacaaaaaat atctgcaaag ataaaacaaa aggattttcc tgcagctgga
    24901 agcattcata cgaattcatt atagaaagta ggatgttttt tatgtgcgga attgttggaa
    24961 tgattggatt aaaaaatgta acaccaggat tgattgacgg tctagaaaaa ttagaatacc
    25021 gtggttatga ttcagctggt atctttgtct cagatggcac aactgattat ctggtcaaag
    25081 cacaaggcag aattcaaaat ttgaaaaaca aaataaatat tgatactaca ggaaatatag
    25141 gtattggtca tacacgctgg gcaactcacg ggcaaccttc tgaagaaaac gcccatccac
    25201 atacttccca aagtggacgc ttcgtgctcg ttcacaatgg cgtaatcgaa aactttgaag
    25261 aacttaaaat tgcttattta gcaaatgacc actttattgg tgagaccgat actgagatta
    25321 tcgctcatct aattgaaact ttcgcgaagg atactacaac gcaagaggcg tttttaaaag
    25381 ctttgcgggt catcaaaggt tcttacgctt tcgctttgat tgatcggaca gcacctgatg
    25441 ttatttacgt cgctaaaaat aaaagtccac tattaatcgg actcggcgat ggattcaatg
    25501 tgattgccag cgatgcaatg gctatgcttg ctcatacgaa ggaattcgtt gagattgaag
    25561 atgaagagat ggttaccgtc acatcagaaa aagttattat tcaaaatttt gcaggtgaca
    25621 tcgtagaacg ttcttcattc gaagcacaag tagattcttc agatatcgag aaaggtacct
    25681 atccttttta tatgctgaaa gaaatcgacg aacagcctat tatcatgcgt cgtatcgctc
    25741 aaaaatacgt aacagaagac aatcgtgtct ctcttgatca aaagctggtc gatacattgt
    25801 ctgatagcga tcgaatctat atcgttgctt gcggaactag ttaccatgcg gggttagcag
    25861 gtaaacagac tctggaaaaa ttgacacaga tccctgttga ggttcattta gccagtgaat
    25921 ttggctataa cacacctctt ctttcaaaaa aaccattctt tattttcttg agtcaaagtg
    25981 gcgaaacagc agatagccgc caagttctgg taaaaatcaa ccgtctcggc tacccttcac
    26041 ttacgattac aaatgttgct ggttctactc tttcgcggga agcatccttt acactgctat
    26101 tgcatgctgg acccgaaatc gcagtagcat caacgaaagc ttatactgca caaatagctg
    26161 tactaacttt attagccaaa gctatcggtg ataaaaaaga atttgaaact tctcttgtat
    26221 tcgatatcac tcatgaattg agtttagttg ctaatgcgat ggaatcggtc atcgcccaaa
    26281 aagattattt agaagagttg gcagaagtat atctcagcga tacaagaaat gccttttaca
    26341 ttggacgtgg cgctgactac aacgtttctc ttgaagctgc tttgaaattg aaagaaatca
    26401 gttatatcca ggcagaaggt tttgccgctg gcgaattgaa acatggaacg attgctttga
    26461 tcgaagaagg aacaccagtg attggaatca tcagtgaaga agtgactggt gcgcatactc
    26521 gaggaaacct gaaagaagtc gaaagccgtg gcgctaaaac actagttatt gtctcagagg
    26581 gattagaaaa ggaaagtgac cagctggtgc taccaactgt ccacccttat ctaactactc
    26641 taaccacagt cgtaccgact caattacttg catattacgc aacgctatta cggggattcg
    26701 acgttgacaa accacgtaat ttagcgaaat cagtgacagt agaataataa aagaacaagg
    26761 accagtgtca gacatctgac actggtcctt gttcttttat tttttatgaa aatatctctt
    26821 aatccagtcc gatttcttct ttgactactg ccgcgatttt atcgacatag taattaactt
    26881 tctcatcagt aggcgcttct gccatcactc gaagtaatgg ttctgtaccg gaaggacgaa
    26941 ctaggattct tccttctgat cccatttcag cttctgcctc ttcaatcact tttttgattg
    27001 ccggaacatc catcgcaccg tttttatcac ttactcggat attcactaat ttttgcggat
    27061 agattgttac ctctgctgca agctctgata atttcttacc tgtctgtttc atgatattca
    27121 gtaactgaat tcctgaaagc atcccatctc cagttgtgtt aaaatctaag aaaatcatgt
    27181 gcccagactg ctcgccgcca aagttataat cgtttttgcg catttcttct acaacgtaac
    27241 gatcaccaac ttgtgtaacg acatctttca aaccgattcc ttccaccgct ttgtggaatc
    27301 ctaagttact catgacagtc gtaacgatcg tatctttttt caatcgattt ttttctgcca
    27361 agtatttagc acagataaac atgattttat caccgtcgac aatgtttcca agttcatcca
    27421 ccgcaataat acgatcgcca tcgccatcga aagctagtcc agcatctgct cctttttcaa
    27481 cgaccatctc agctaaacgt tctggatggg tagagcctac gccatcattg atatttaacc
    27541 cgtctggatt tgtgcccatg gtataaaaat ctgtttcaag atcggcaaac aaacgattaa
    27601 cacttgttga agttgctccg tttgctgcat cgacacaaat ggtcaaaccg gaaaggtcac
    27661 cgttgatcgt ttgtacaagg aattgagaat atttcaataa gccttctgga aattcttcta
    27721 cagttcccaa tccttcagct gatggacgag gcaattcatc cacttcagca tctaataatg
    27781 cttcaatttc tagctcttgg tcatctacta gtttaaaacc atctgcaccg aaaaatttga
    27841 ttccattatc ttgtgctgga ttatgagaag cagaaatcat gacccctgcg cttgcttttt
    27901 gcaaacgtgt cagataagca acacctggtg tagagatcac acctagctgg aacacttcga
    27961 tacccaccga taaaagtcct gaaatcagcg cttcttctaa catttgacct gagattcttg
    28021 tatcccgccc tactaacacg cgtgggcgtt ttccttcttc ttcatgctgg cttaggacat
    28081 aacctccgca ccgtcccagc ttaaatgcaa gttctggcgt aagttccttg ttggcttcac
    28141 cgcgtacgcc gtctgttcca aaatatttac ccatagttgt aaaattactc ctttaactta
    28201 attttctgag gaatttgttc tgctcatttg tgttgattcg cttgtttctg atgtttcttc
    28261 tgtacttgtg gtcgagctgc ttgtcccaga ttccgaactg gaagaaatgg ttgttgtcga
    28321 tggttccgtt cctgaatcag cagatgaaat acttctactc gtacttgtat tttcttgtgt
    28381 ccgactgctc gtcgaagcag agctactgct tgacgtttgc aacacgggtg taatctccac
    28441 actaactgat tcgggcttga tacttaaccc ctcttctact ggtatttcaa ttttacgagt
    28501 tgttcgatga ttgatacctg agacattgat cggtacagca atactgtcac cgatcgatgc
    28561 aagcaaatcg ctagagccgc ttacttctgc ttccagtttt gaaaaactga aatcatagct
    28621 ttctaccccg gcagctggtg ttccttgctg tatcccataa agtcgtaccg acttgcttgg
    28681 cttgatcgaa tcagctctca ctgttacttg cactggatca gagacgatac ttaatacttc
    28741 ccctgctgca ttcagcgcct gtattgggac ctcactatta atcccatcat ctgtgaccat
    28801 cgaagcgtcc acatcagcta ctactcgttt gatttctgcc aatgttttgt cacctgtagt
    28861 gatagagact tttttaggac taacggaagt atcatcaagt tcatatccag atggtgtgaa
    28921 cgttgaagga atcaacgttt ccacatcgaa atcttttatt acctttttct caaccgttac
    28981 tgtaatagtc gatggttcga tctttgctgt gacagcactg ctcaaatttt gaatttttag
    29041 cggaacctca tgtgttcctt cgcccagctt agtgatatct gctgtgacgc ggaacatccg
    29101 tgtatcttcg tttgcctctg cattcaactg aactcggttt gcactggaaa gtttgacagt
    29161 cactgcattt tcatatccat ggatatagta gcgctttgaa tcataagaaa tcttgattgg
    29221 caccccagta actgtttgat tatacgcttc atttccagaa agcgtatttg gaacggactg
    29281 gccatttgca ttgaagtaca gcgcaatact gaacagcaat gcaataaaac tgtaaagaat
    29341 gcttgtccgt ttctcttttg agatcatttg cgatcccctc ccttagtcat accgtctagg
    29401 aaagattgaa tcacgttttt cttgtttttt gttttctctt cagcaatcag ttctctacgc
    29461 aaaattgcca aatattcttg ctgggataag ccagccatca gttcattatc taatgtaatg
    29521 ctgacatccc cagtttcttc agagacaacg atggtaatcg cgtcgctgac ttcgcttata
    29581 ccgacagctg cccggtgtcg agtcccgaat tcttttggaa tcaacatact ttctgataga
    29641 ggaagatagg cgcttgctac tgcgatcttt ccttctttta caataactgc accgtcatgc
    29701 agcggtgtat taggaataaa gatattgatc agcagctctc cagtgatatc tgcatctaat
    29761 gcaattcctg tctcaatata ctcatctaat ccagtatgcc gttcgatagt aattagcgca
    29821 ccgatttttc gcttggacat atattggatg gctttgtcaa aagacaagat catcttctca
    29881 tcttcctgct gttcgctttt tgattgtttg aaaagcgaac tgcgtccaag atgttccagt
    29941 cctctccgga tttccggctg aaaaatcacg atagcagcta tcaccccgta cgtaatgact
    30001 tggttcatca gccaagacaa ggtgtgcaga ccaatgattt ccgctacgat tcggataaca
    30061 ataaaaatag ctacaccttt cagaagctga atcgcttttg tgcctcgcag cagttggatc
    30121 agtttgtata caagatacca gacaaccaga atatcaagta tgttcaccaa aaaattcaga
    30181 gagaacaagt caccggaaaa catttgctgc caatagtcca aattaaaaag ttccccgatt
    30241 tgaaatgaca taaaaagcct ctcttctctt ttgagatttc ctctctagta tatcatatgt
    30301 tttaatttta gaggctgatc tgctcgtatt tttcttgaga aaaacgcata attttcagga
    30361 aaattgcaag tatgcgtttt atttttctct gcaataaata tttttaaact tcagagaatt
    30421 attcattata ttttttctgt tatttttcct ttttttcttt tgtagcttgt tccatttttt
    30481 tacgtcgttc tgctctggca gctcttcttt cttctgcttc aaggtccgtc gttttttctt
    30541 ttctagtctt aacgggtaca tcgatagatt cctctggttc aagtcgtttc ttgatttttt
    30601 ctaattctcc cgtcagtgca gcatagttgt aaaaacgtgt tttagcgtct tggatagtct
    30661 tttcaaataa agcttttgca agatttggat ttgcgttctc tagcgaggaa aagcgaactt
    30721 gtttcctcat gaaatcaggc atagcagaga aatcaggttt tttgtagtcc aaagtcattg
    30781 ggttcttgtt tttttcgacc attgcagggt tatatcgata taatgaccaa tagccagaag
    30841 aaacagcttc ttttgcttct ttaagcgatt ctttcattcc accaagcaaa ccatgtgtga
    30901 tacaaggcgt ataggcaatg attaaagaag gtccaggata cttttctgct tcttcaaaag
    30961 cctttatcgt ctgcatttga ttggcacctg aagcaatttg cgccacataa acatttccat
    31021 atgtcatcgc catcattcct agatctttct ttgaagcata ctttccacta gccgaaaatt
    31081 tagcgattgc cgaagctggt gttgcttttg aggtttgacc gccagtattg gaatagactt
    31141 cgttatccaa aacaagaata ttcacgtctg ctccgctggc aagtacatga tcgatcccgc
    31201 caaaaccaat gtcgtaagcc catccgtctc caccaatcat ccattggctg ttttttacga
    31261 atagatcctt tctcttgtat atatcctcca gttcagggac aattgatcgt tcgctttcga
    31321 tcgcagcttg cagttttgct gctctttgct gcgttccttc gctctcaaac atgtgtacca
    31381 accagtcttc catcaattct cgtagttctt ctgatacttt tggcaatact tctttgattg
    31441 aacttgccaa agcctcgcga cgagtctgtg ccgctagaag cataccataa ccaaattcag
    31501 cattatcttc caataaagaa tttgaccatg caggcccttg accacattca ttggtcgtat
    31561 aaggagaagc aggagaagca ccgccccaaa tagaagaaca gcctgtcgca ttcgcgatca
    31621 tcattcgatc accgaacatt tgtgtcaata attttacata aggtgtttcg ccacagcctg
    31681 agcaagcgcc cgagaattca agaagcggtt gattgaattg tgtagacaag acagtattct
    31741 ttcctttgac tggatttgct ttttgtctta atgtcattgc aaaagcccaa ttcacagctt
    31801 gttctttctg ttcttcataa ggcttcatca ctaaagcttt ttcttttgcg ggacaagcct
    31861 ctacacacag accacaacct gtacagtctt ccactgaaac ttggatacgg tattttacgc
    31921 cgtctgcgcc tcgcatttct ctcacgataa atccttctgg cgcttcttcc atttcctctt
    31981 catctgctaa aaatggacgg atggcagcat gaggacaaac aaaggcacat tcattacaca
    32041 tcgtacagcg atcactaatc cactcaggta cttctaaagc gacgccccgc ttttccatag
    32101 aagccgttcc taaaggcatt tcgccagttg tcattccatt tgctacaagt gtaccaacag
    32161 agagttgatt tccttcctgt gcattcactg gttcaagtat ttcttgtata taaccagtgt
    32221 tccttttatt gattttttca ggcatctcca acgttctcca atggtctggg atcgcgactt
    32281 gatgaagcag ttcggtcgtc cgatcgattg cttgtatgtt tttttctacg atagccattg
    32341 atttatgtcc atagcttttc agggcttctt cttttagcag cggaagcacc tcatcaaaag
    32401 gaatgatgtc agctaattta aaaaaagccg tttccattgc cgtattgatc cggcgtccca
    32461 aaccgacttc ttgggccaat ctcatggcat tgatcgtata gaatctaatg ttattttcag
    32521 ccaagtaacg ttttaatttt aagggcagat gcgtttctaa ttgttcgtcg ctccaaagag
    32581 tattcaataa aaaaatgcca cctggcttca atcctttgac caagtcatag ctatgcaggt
    32641 aagctggtgt gtgacaggca acaaggtccg catgctcgac aagatacgct gaacgtatcg
    32701 gcgtatctcc aaaacgaaga tgagagacag tcagaccgcc agattttttc gaatcataat
    32761 aaaaatagcc ttgcgcatat ttatcggtgt gatctccaat aatcttgata gctgatttat
    32821 ttgctccaac tgttccgtca gatccaaatc cccaaaactt ggcctggaat gtttgcggtt
    32881 ctgtcaaatc cagactttcc tttggttcca aagattgata agtcacatca tccacgatcc
    32941 ctatggtaaa ccgttttttg cgtatgcttg ggtctttttt caattcatca aaaactgctg
    33001 agatttggtc gggagtcaca tctttagacc ccaatccgta gcgaccacca ataacagccg
    33061 ggcgaagttc agaatcatat aacgcacttt gaacgtctaa taagagaggt tctccacctg
    33121 cccctggttc tttacttcga tcaagaacag caattgtttt tactgtatga ggcaactttt
    33181 gcacgaatgt ttccactgga aaaggacgat aaagatggat attcaaaaaa cctgttttcc
    33241 gtccgtttgc attcaagtaa tcaatcgttt gttcaatcgt ctgtgcaact gatcccatag
    33301 aaacaatgac ttcttccgca tcgtctgctc cgtaataagt caccaagtca taagaagttc
    33361 ctcgtaatcg gttgatctct ttcatatatt tttgaacgat ttcaggtaat ttatcataat
    33421 aggggttgat cgtttcccgt tgttgaaaat ggatgtctgg attctggttc gtaccactaa
    33481 cagtcggatg attgggattc atccctcgtt tacgaaatga agctaatttc tcctgattga
    33541 tcagaggagc taattcttca tattccaaaa cttcgatttt ttgaatctcg tgactcgtac
    33601 ggaatccatc aaagaaattc aaaaaaggta cttctccttc aatagaagca agatgggcaa
    33661 caggtgctaa atccatcact tcttgtacac tgctttcagc taacatagca aatcctgttt
    33721 gtcttgcagc catcacgtct ccgtgatctc cgaaaatatt caacgcattt gtcgtcactg
    33781 cccgactagc aacatggaaa acacttggca gtagttcccc agcaatcttg tacatattcg
    33841 gtatcatcaa aagcagccct tgagaagcgg tatatgtagt tgtcagtgca ccagttttca
    33901 atgatccatg gaccacacca gctgctcctg cttcggattg catttctact acttttactg
    33961 gttgaccgaa tacattttct tttcctgctg ctgaccattg atctactaat tcagccatgg
    34021 tcgaactggg tgttattgga tatattgcag caatctcggt aaatgcataa gaaatatatg
    34081 cggctgccgt attgccatcc attgttttca tttttcgcat gattcatcat ccttgttcat
    34141 attctctctt attatctgtt ccattataac gtaaaacatt ccattttttc ttttatttat
    34201 aaaaatattt cacatatgtc tatttgaatt ggtcatctaa tttttagaca aacagtctaa
    34261 atcgttgcct ttttttgtca gacatgtata aactataagc gaaagaggtg ttttaacatg
    34321 gatatatcag tgattgatgc aacgaaaaca aatacacaaa aaggaatcct ttatggttca
    34381 tcgaatgctc ctaaaaaaat ggtcgaattc atcaacctat cttgtccgta ttgccgacaa
    34441 tggtttgaag aatcttatga gctattagaa gaagcagtcc aaagcgggca attgcagcga
    34501 gtgatcaaat tgttcgataa agaaaaagaa agcttgcagc gaggaaacgt gatgcaccgg
    34561 tatctcacaa tcagcgatgg tcaaaaagcg atcaaagaga tcaaacaaat attcgatact
    34621 caagacgagt ggaaacatct ttctctccaa gaagtagcgg attttgcagt agataaacta
    34681 aagctgacag aacaaaaaga cgaacagctg agccaagctg tcatcaacga agctgagcaa
    34741 gcacatattc gatttgttcc gactgtcatc ttgggtaaag aaatctttga tgaatctatt
    34801 tcaatagagg aattaaaaga attgatccaa acaaaataaa aaaataagga gcgtaggaca
    34861 aaagtaaaga acacttttgt ctcacgctct aaacacctat aaacggcgga agcagaagca
    34921 actccttctt atttcttgga gttaaacgct tctgtcccag cctccttatt ccgaataaat
    34981 ggtgtccaaa agcttgcatc tgccagatta tctcatttct tatgacaagc tgagcaaatt
    35041 atctccacca aataatttat tcatattttt gacttgcgca tactaactaa ttcttaaatt
    35101 ttcatacaaa atttctcaaa ttcccttaac gagtaatatt tatgtacggt gattcacact
    35161 agtatcccaa tcacttttta agttatcccg atttcaattc tacttcaaaa atagttgctt
    35221 ttatgtatgc tgttagttga ttagatggtc gtttgttgag ctaggtcgtt tattttaaac
    35281 aagttgtttc tttagcttat acctatctcg tctaccaaac gcaagatggc aaacacaaat
    35341 gcatacgcat ctgtatttgc cactttgatt cgtataaagc taacaactcc taactcctct
    35401 catttaatag ttgctctctt ttttattcag atacattttt gctgatcaca gcctagtatt
    35461 tcaatggttt tgaacactaa accaaattgt tccattacaa cattcactgg ctgatgaaat
    35521 ggattaatag atttcgatta ttcaaagcga ttttctattg gtggattgtg ttcccaacta
    35581 acaagacgga ttccaaagtc attatataac tcttcaagag ttaacccttt cctgttccca
    35641 atagatgtat ctctcccacg ctcgatataa aatatggagt ttcgaatgcc aagacctggc
    35701 aaatctttcc ttgttacagt tacactgcta ctaacttccc catataaaat ccagctcctc
    35761 caaaatgata atatattttt cgaccatcat ctaactcatg taacaataat tgttgtgccc
    35821 tcttgtcact cgccaaatgc aaacgccacg tgcgatacca tgccatgcta cctgcatctg
    35881 cagaaaatcc atcaaataat gcaacaattg tatcttctat cacaatcctt tccccatcta
    35941 cttcatattc taatctaaaa tcaaactccc ctcgggtaat cgcaggtctt ggtggcttag
    36001 gacccaatat tattctactt aaaaccgcat tcgttcccag atgaaacaca gctataacaa
    36061 caataatgat taatattttg tttctatttg ataaatttct cattattcgc aaatcacctc
    36121 tacattctca catactagtt cgtaatactc ctttaatatc aaaatatcta ctgatcgtaa
    36181 taatattcgc tatgaattcc tactactcgt ataagaaagg ccaaatcatc agcttttact
    36241 tgctgatgat ttggcttgtt tctatttatc gtaattgttt tctaatcacc aaaattgtta
    36301 acaatcggtt cagatatttc ccaattcact agccttatat tatattgatt taacaattct
    36361 tcttcattta ttgtattttg atcaagcgga ccaaattgtt ctaataattc tttgtcatgt
    36421 aaaatatccc ttctagtaat gcctctttcc ctttcaatga aagaagcact aggaaaagta
    36481 tgattataac cattcaacct atctcccatg taataattag caggtcctgg aaaataaaat
    36541 atttgcttat tatcactaac ttctaacagc aacactaaat cttctccgct ggctaaacga
    36601 cttgtccatc tacgatatct ccctatgcct tcgttcattc caaatccatc aaattcaact
    36661 attaacgtgt cttctatcac atgtaattct tcattcaatt catactcaag tctaaaagga
    36721 aattctccat atgtaatctc tggcaatggt ggtgctggag ttctccaaat aaatacaata
    36781 actgtgaata gaaaaaatcc aacgatcgat ataaatgtta tgagacctgc tcgaagtaaa
    36841 acccgatata ataaaaatgc tgtatttcgc tttgctatat ctagaaaaat aaaaagtact
    36901 gcaatcataa tgatagtaaa agttgtaaaa aaacgcattt ttgccctccc tttataaaca
    36961 tcgattctca aattctaaac caaatcttgg tagttcaagt tcaggtataa cgtattccaa
    37021 ccatacatct caattactat ttatgaactt atgattatca ctgaataaat cactccatcc
    37081 ccttgttaaa acttctacat taattacacc tggttgtttt cgactagcta atccaatgaa
    37141 cttatcaacc atccttatcc accaaccacg agaatcttga ccagaagtaa gaagagcagt
    37201 tctcaaggat gatcgagcag ttccttccag ttttttaatt aatcaggcca gttatagata
    37261 aatacttgat cttatctata tcaggacttc tatattaagc aaaaatttat gtacaaaact
    37321 aaaatcttca ttcagttgtc tccccgcttt ttatacgatt gtattaaaaa caatagaagc
    37381 ctttccaaat tatgttactc cagtaaagat ggaattatag tttgacggaa gaaaagcaag
    37441 tataactttt aaaaaaaact gatacattcg catctcctgc ttcatgtatc agttttttct
    37501 ttggattaaa ctgcttcttc acgcagtttt tccgcaaatt catttatttt gcgaattcgg
    37561 tgattaattc ccgattttga tatcgcacct gagggaatca tttcccctaa ttctttcaag
    37621 ctgacctctg gatgttgaag ccgaagttct gcgatttcct gaagtttttc aggaagggca
    37681 tgtagaccga cctttgcttc gatcaattcg atgttttcga tttgtttaga agccgcatca
    37741 atcgtcttgt tcagatttgc tgtctcacaa ttgactagac gattgaccga atttctcata
    37801 tcccgtacga ttcgaacatc ttcaaatcgg agcattgagt tggttgcacc aatcaatgtt
    37861 aagaaatcag caattttttc tgctcctttt aaataagaaa tatagccatt gcgccgttca
    37921 agcgttcgag catgtagacc gtaataattt aacatatcgc aaatgtcttg gttatgctgt
    37981 tcataaatag aaaatatttc caaatgataa cggctcgttt ccggattatt gactgaacct
    38041 gaggccataa aagcaccacg taagtaagaa cgcatttttt gcgaatttcc cataatctcg
    38101 tccgaaacgt gtccattgaa catcatacca tccatgatat caagatccat caacacatct
    38161 tttgtgcctt gcttcaaccg gacgatatag acattgttct tttttaattt cattttttta
    38221 cgaacgagta gctcacttcg tacctgataa tgatctttca aaagagaata gatacgtctg
    38281 gcaattgcag cattttctgt ttggacgttc aaaataaact gctggttcac aatacctaaa
    38341 gagccgttca ttcgcagcaa agcagctaat tccgctttcg catgttctcg atgaacttcc
    38401 aaaccagtta gttctttctt cacatctgca gcaaatgaca tcctattctc ctcctttctc
    38461 caacaataaa ttgcttaata ttttgaaccg aaaactaaac ggaacaattc ttctactact
    38521 ttgtctccat cgtggaagac accaccatct ttcaattcca aaaagtcggt ggaaatcact
    38581 cgacatcctt catttctcaa tccgttgaag tcgtgtgcga cttgtactag atactcatca
    38641 tagatctctg ggtccatata atcttcaggc actttttccg tattgaccaa tactgtatcg
    38701 ataaaaggct gattcaaatg cttgtgtaaa acacgtatat gatcggcatc tgtgaaatgt
    38761 tccgtttccc ctttttgggt catgatatta cagatataga cggtttcagc agttgtcttg
    38821 agcattgctt cgccgatttc tggtatcact aaatttggaa ggatactggt gaataaactt
    38881 ccgggtccca aaacgaccat gtcggcgtcc ataattgctg aaacgacttt tcgagccgct
    38941 ttggcttcac ttttatcatt tgtgttgcag acatacacac ggtcaatggt tttacggtcg
    39001 atcgcgattt tagattctcc tactgcagtc gttccgtctt taaagacagc atgcaaggtc
    39061 aaaggagttt ctgaagaagg ataaacatgt ccatctacat gcatcatttt tgctaataat
    39121 tgaatcgcct cataagtgct tccccgcatc tcggaaactg cggcaatgat cagattaccg
    39181 atcgtatgat tggctaaaaa ctgatcttcc tttttgaaac ggtattggaa aatatcttca
    39241 tagaattgtg gcatatccga taacgccact aagacatttc tcaagtcacc aggaggagcc
    39301 atagcgatcg attctctgat tgcaccgctg cttcccccat catctgcaac agtcactact
    39361 gctgtgatat ctgctccttg atttcgcaaa cttttcagga taactggcag accagtgcca
    39421 ccgccgacca ctacgatctt tggttttctt atccggtagg ttttcatttt cacgaccggt
    39481 tcaccgtctc tttcctttta tccttgtccc gatgtgtcac attgacttta tagtttttcc
    39541 ctaaagcatt tgccaaacgt tcagtcaatg ccacagaacg gtgttgaccg cctgtacagc
    39601 cgattgcaac tgtcaagctg ctttttcctt ctttaatgta tccaggcaat acagtttcca
    39661 gaagatccaa aaatttatgg tagaatgatt gcgtttcatc aaaggacatc acataatcat
    39721 agactggctg atcttttccg gtcaatggtc ttagttccgg gatataatga gggttgggca
    39781 aaaaacgcac atccatcaca atatccgaat cgatcggcaa cccgtattta aaaccaaatg
    39841 atacaaattc gatccgaaat ccttggtctc cctttgattt gaatgactca ttgatttttt
    39901 cacgtaattg tcgtggtgtc aatgtcgtcg tatcaatgat cacggaagct tgggctttca
    39961 agtcgtctag tatcgcccgt tctttacgta tcccttcagt gatcaaaccg tccatcgcca
    40021 ttggatgtgc tcgtcttgtt tctttgtagc gagccactag ttcttcgtct gaagcatcta
    40081 aaaataagat agaagtgtcg atgaagttcg tattttcgat ttcgatcagc atgttttgga
    40141 tttcttcaaa gaaggaacga gaacgtaaat ccactcctaa agccattttt gtgactttgc
    40201 ctgattcttt gattaattcc cagaattttg gaatcaatcg aggcggcata ttatcgatac
    40261 aaaaatagcc catatcttca aaactttgga tagctactgt tttccctgca ccgctcatac
    40321 ctgttatgat gactaattgc aaattatctg tcataattac gaactcctct cacattgtgc
    40381 atagtaaaat tatatcattc cgggcgtatc attaccagtt ttctttttct tttacaaaaa
    40441 gatttggacc aaatatcttt taggatattt gccccaaatc caattttttt acttttccga
    40501 tgctgtatcc aatgccttgc ttctttcagc tagcgctcgt tcaatcgtcc gatctgctaa
    40561 aatagattgg gcatcgatca ctggataatg gttatcttca gcaaattcat gcatcaatga
    40621 caattctgtg caacctaaga taactttttc acagtttagg cgctctaccg cttcttctaa
    40681 gatttcgtaa tatagctctt gattcaaata atccgattcc ttgatctcat gatagatcag
    40741 ataattgatt ttttcttgaa gaacagtatc aggaatcact gtttcgaatc caagattttt
    40801 gacttcgcgt tcatagattc cagctttcat acttccttct gttcctagaa tcgctactct
    40861 ccctgtcgta tgctgacgga caagttcatt tgctgcttct cttggcatat gaaggatagg
    40921 aatatccgtt gccgcttgta attcttcaaa aaagtagtgt gcggtgttac aagtcaacac
    40981 aatgaagttc ggctgcaata aattttgttt ctcaatatca tccagcagaa acggcattgg
    41041 attttcttct gatcgatcta aaatataagc tgtacgatca ggaactgttg catgattgaa
    41101 taagacataa ttcaaatatt cttgatcttt tgtagctttc gttcggtggt tgatcaagcg
    41161 aacaaaactt tctgtcgcca tcgtgcccat tccgcctaaa atactgaaaa aattctccat
    41221 gaactagcct tctttcacat gaaaatactt tttgaatctt ggaatatagt tatggaacat
    41281 atatttcatc aacaaccagc gcttgattga ccggtctttt tcataaaaca cagtagtacc
    41341 gtaacgattt tctttgatca tttgctcagc tagttttttg tcttcatttt cccgagcata
    41401 ttctaaaaag atttttttgg gcacacccat ccataatctt gctttatctg attgattcgt
    41461 tccataggtc gtttcaacaa agggtttgtt aaacactcga tcttctgtga cgaaacgagc
    41521 cagattcaag ccgttcaatg tcacaaagaa actgctacga ccttgtctca aattgatttc
    41581 aaataattta tattctccat ctctaggatc atacttcata tcgaaattgg caaagcctgt
    41641 atattcgatt ttttccaaaa atgccttaat ggtttgatag attttttcgt tataatctgg
    41701 catgatcaca acgtaattac caatcgatgc tggtgtagga tcttctaaca gtggatgacc
    41761 aagacacatc atccgaactt gatgatcttc atctacatac gcatttagca cacgcatatt
    41821 actatcatca cccgggataa aatcttgtac aatcatttca cttttataac cggcttcgta
    41881 tatccggcct aaaattaaat cgaactcttc tctagtctct aaaataaaag ctttttttct
    41941 tccttcaaat tggacagaaa gatactccac actgtttgca ggtttcaaag caactgggaa
    42001 ttcaaaaggc aattcctgtt ccaaatgtcc atttactaac atttcttctc tgacgatcaa
    42061 tgttttagga tacggaagat catattcttc acaaacttca taaaagctta ctttattgat
    42121 cagtctttca aataaagaat aatcaatata tggacagata aaatattctg acagctcttg
    42181 tttatgttgg gagatcaatt ctgcatatcc atcaccacaa gcaatcaaaa gatatttttt
    42241 actcttatca gaatatcttt cttttgccaa acgaagcatc gtctcgataa acacagggtc
    42301 tttatcaaat cctggaataa cttctacatt gacaatttta ctgtagcggg taggggctaa
    42361 ctgatccgaa gcatatgctt ggcagatttt gccgtatgct tcattaaaag aacgtgccat
    42421 accataaacg ttcatgtcgc ttcctaataa tatagggata aattcttcat tttcaatact
    42481 gttcatcatt atctctcact tactcataat ctcttgtttt tcttttccaa ctacacaagg
    42541 aatatcatac cataaagaaa aaagcaatca aaggaaactc ctccgattgc tcaattcttt
    42601 tttgaacgga ttgaacttat aggaagtttt tgtctatacc aatcagtgta gaaagaacta
    42661 ccatgtaatg attctagaac actgatatta ataaaacaag caaaaattca tttttttatg
    42721 tttttttaac tatctcagct atctatcctc tttataccat tatttttttc aaatagtgac
    42781 ctgtataact ttcttttacg ttaatgattt cttctggtgt tccagtcgca aggatcgttc
    42841 ctccaccttc tccaccttct ggtcctaaat caatgatata atctgctgtt ttgattacat
    42901 ctagattgtg ttcaatcact aaaactgtgt taccagcatc tactaatctt tgtaatacat
    42961 gcaacaagcg ggcgatgtca tcgctatgaa gtcctgtcgt tggttcatct agtatataga
    43021 aattctttcc attagagatt ttgtgcaatt cactggcaag tttcatccgt tgtgcctcac
    43081 caccggacaa tgtcgttgct ggttgcccca tcgtcacata acctaagcca acatcaacaa
    43141 tcgtttgcag tttgcgatga atctttggaa tgtgcttgaa gaattctaca gcatcttcta
    43201 ctgtcatttc caaaatatca gcaatgcttt ttcctttgta atgcacttct aatgtttcag
    43261 agttatatcg tttgccatga catacttcac aaggaacata gacatcaggc aaaaagtgca
    43321 tttcgatctt aataattcca tccccgcgac aagcttcaca acgaccgcct tttacgttga
    43381 aactaaaacg ccctttctta taaccccgca tttttgcctc gttcgtttga gcaaataacc
    43441 cacggatatc atcaaataca cttgtataag tcgctggatt acttctcggc gtccggccga
    43501 ttgggctttg atcgatatcg atgatctttt cgatactttc gtagccggaa attgtcttga
    43561 atttacctgg cttagcagaa ttcttattta atttttgcgc taacgatttc tttaagatac
    43621 tattgaccaa tgtactcttt ccagaaccag acacacctgt gactgctaca aattctccta
    43681 atggaaattc aacactaata tttttcagat tgttttctga agcaccagtg atcttgattg
    43741 ctttcccatt acctttacgg cgttcttttg gtacagggat tgattttttc cctgaaagat
    43801 atttccctgt caaagaattt tcatcattag ctacttcttc aggagtgcct gcagccacga
    43861 tttgtccgcc ttgttctccg gccccaggtc cgacgtcaat cagataatct gctgcccaca
    43921 tcgtatcttc atcatgttcg actacaacga gggtgttgcc aagatcacgc attttcttta
    43981 atgagccaat cagtcgatcg ttgtcgcgtt gatgcaatcc gatcgatggt tcatctaaaa
    44041 tatacaaaac tccagaaaga ttcgatccaa tttgtgtagc caatctgatt cgctgcgctt
    44101 ctcccccaga taatgttccg gaagcgcgac tcaaagttag atagtctagt cccacatttt
    44161 ccaagaatga aagtctgtca tttacttctt ttaaaatcgg tttggcaatt actttctctt
    44221 gttcagataa cgttaagttt gagaagaaat ctactgcttt ttgaatagac aactcatttg
    44281 tttgcccgat attcgttcca ttgatttgaa cagatagagc ttggggattc aatcgataac
    44341 ctttacatgt ctgacaagtt aattctgtca tataaagacg catttgctca cgcgtgaaat
    44401 cactgttcgt atcatgataa cgacgctcga tattcttcaa taccccttcg aaagtcgttt
    44461 ctacatcgcg aacgccacca aaatcatttt cataatggaa atggaaaaga tcatctgtcc
    44521 cattcaagat gatatcctga tgctcttccg gtaattcttc gaatggtgta tctaaatcga
    44581 taccgaaact ttcacaagcc tgcgctaaca tttgaggata atactgagag ctgattggat
    44641 tccatggaac gatagcacct tgactaagtg tcttcgttct atctggaatc actaaatctt
    44701 gatcgacttc taatttgaca cctaaaccat cacagtctgg acaagctcca aaaggcgcat
    44761 tgaatgagaa cagtcttggt tctagttcgc caacagtaaa tccgcaataa ggacaggcat
    44821 agtgttcgct aaacaacatt tcttcttcac cgatcacgtc aaccagagca tagccgtctg
    44881 ccaatcgcag agccgcctct aaagaatcaa atagacgaga acgaatccct tctttcacaa
    44941 tgatccggtc gatgacgatc gcgatatcat gttttttgtt tttctccagc tcaggcactt
    45001 cacttaaatc atagatctca ccatcgactc gaacgcggac gtatccctct ttttggattc
    45061 gttcgaatat ttttttatgt tgtccttttt tctttaccac gatcggtgcc aacacttgga
    45121 tctttgtgcg ctcaggcaaa gccattactt gatctgccat ttgatctact gattgactgg
    45181 taatctcgat atgatcgttg ggacaaatcg gatgtcctac acgtgcaaac aacaagcgta
    45241 aatagtcatt gatttccgtc acagtaccta ctgtcgaccg aggattttta cttgttgttt
    45301 tctgatcaat agaaatcgcc ggactcaatc cgtcgatgct atctacgtcc ggcttatcca
    45361 tttgtcctaa aaactgacga gcataagcag aaaggctctc tacgtatctc cgctgccctt
    45421 cagcgtacag cgtatcaaat gctagggaac tttttccaga accggacaaa cctgtaacga
    45481 ccacgaattt atctcgtgga atcgttacat ctatattttt caagttatgg gcacgtgcac
    45541 cgtgaatcgt aattttatcg ttagccatgt tgtcctccta ctttgaagct tttaattcta
    45601 aaatcatatc tcgcacatta gcggctgttt caaaatccaa tgctttcgcc gcatttttca
    45661 tttctttttc taatttcatc aataacgtat ctttttcttc tttcgtcatt tcttcatacg
    45721 agacttgtac cgcttctttt gtatcatctt ccgattcttt cgtaatagaa atcaaatctc
    45781 ggatttcttt aatgatcgtt tttggaacga tcccatgttc ttcattgtac ttctgctgaa
    45841 tcgtacggcg tcttgatgtt tcatccatcg ctagacgcat agaatctgtt actttgtctg
    45901 cgtacatgat tactttacct tcttcattcc gagcagctcg tccaatcgtt tggactaagg
    45961 aacgttcact acgtaaaaag ccttctttat ccgcatccaa gatagcaaca agggaaactt
    46021 caggtacgtc taatccttca cgaagcaaat tgattccaac taacacatca aattcaccta
    46081 atcgaagatc tcgaatgatt tctgttcgtt ccagcgtttt gatatcacta tgcaaatatt
    46141 tgactttgat ccctaattct ttgaaataat ctgtgaggtc ttctgccatt tttttcgtca
    46201 atgtcgttac aaacacccgc tggtcttttt cgactcgttc atttatttcg cctactagat
    46261 catcgatttg tcccatgatt ggacgaactt cgatcactgg gtcaagcagc cctgtcggac
    46321 ggatgatttg ttggataacg gtatctgttt gttcatgttc atatggtcct ggcgtcgcag
    46381 agacataaat gatctgattg acatgttttt caaactcttc taaccgaagt ggccgattgt
    46441 ctaaagcaga tggcaaacgg aaaccgtaat ctactagcat ctgttttctc gctcggtcac
    46501 cattatacat tcctctgact tgaggcatgg ttacgtgcga ttcgtccgcc acgatcaaaa
    46561 aatcttcagg aaagaaatcc aatagcgtat aaggcggttc accttcttta cgtccgtcca
    46621 tatgccgaga ataattttca attcccgacg tgtagcccat ttctaacatc atttcaatat
    46681 cgtaattggt tcgctgttct aaacgttgag cttccagaag tttgttttca tttcgcaaga
    46741 cagttaaacg ctgttctaat tccgctttga tattcgctac ggcatgctcc atatgttcat
    46801 cattcgtcac aaagtgagtc gccgggaaga ttgccacgtg ttccgtttct cctaaaactt
    46861 cacctgtcag tgcattgact tctcggatac gttcgacctc atctccaaaa aactccacac
    46921 gtaaggcacg ttcatctctt gaagccggaa aaatctccac gacatcaccg cgcactcgaa
    46981 aacgaccgcg ctgaaaatct atatcatttc tttcgaattg gatactgact aagtcacgaa
    47041 tcaactgatt gcgatctagt tctgctccct gtcgaatcga aactacttgt ttttggtact
    47101 caaaaggcga acctaatcca aaaatacatg aaacggatgc aatgacgatc acatcatttc
    47161 tttctaataa tgaactagta gccgaatgtc gtaatttgtc gatctcatca tttacacttg
    47221 aatctttttc gatatacgta tcgcttgaag gcacataagc ttcaggctga taataatcat
    47281 aataagaaac aaaatattca acagcattgt tagggaaaaa ttccttgaat tccccgtaca
    47341 gctgaccagc caatgttttg ttatgggcaa taattagtgt tggtttattc actttctcaa
    47401 taagattgga aatcgtatat gttttccctg ttccagtcgc acctaataag atttgtgctt
    47461 ttttcccgcc gactacgcca tccactaatt ggttaatcgc ttcaggctga tcaccagccg
    47521 gttggtattt cgagacaagc tcaaagtgtc gggaggtatc tctctcaatc attcttcgtc
    47581 ctccttttca ttcattcctt ttcattttac gaccaataaa aagaaaagga aagcaaaggc
    47641 attccttact aaaaaattat accacaccga acatactttc gccacttttt tatcttatta
    47701 tgcatgttac gttttataac ataaaatgtt agaaactcgc atttttttca ttttttcgag
    47761 cttacagtct tgtctgattc agcacttttc gcctataata ataaaatatt ggaaatttag
    47821 gaggttgatt atgaaaagaa agcatttatt tcactcattt gttttcttaa tgacaggact
    47881 tgtgacactt gcgtttggac atgttgcaca agctgacaac actacaacgg cagagtcaag
    47941 cacaagcgag acaatgcaaa aaatagagcc caaaaaagac gtatacgtaa ttgccagtga
    48001 ctctgctttt gctccatttg aatttcaaaa cgcaaagggc gagtacgaag gcatcgatgt
    48061 aaaattgatg gaaaacatcg ccaaactgca aggatttaac atccgaatgg actaccgtgg
    48121 tttctctgct gctttgcaag ctttggaatc aggacaagca gacggaatga ttgccggaat
    48181 gactgtaaca gatgaacgaa agaaaagcta tgatttttct gattcttact ttgatagcgg
    48241 gatccaatta gctgtcaaaa aaggagatac ttcgatcaag tcttatgaag atttgaaagg
    48301 aaaaacagtc ggagcaaaag ttggtaccga aagtgctgaa tttttaagcg accatgaatc
    48361 tgaatatggc tattctatca aatattttga tgctgctgat caattgtacg acgggttaaa
    48421 agttggcgca atcgatgcaa tgatggatga ttatccggta atcggttatg cgatcaaaca
    48481 aggacaagaa ttggaaacac cgatcaaaaa agaatctggc ggaaaatatg catttgccgt
    48541 taaaaaagga caaaatcctg agcttcttaa tatgttcaat gaaggattga aagaattaaa
    48601 aagaacaggc gaatacgata agattattaa agaatatgta gcggatggtt cagaaacttc
    48661 cagtgagtct tccactgctg atgaatcaac atttcttgga cttatccaaa acaactatca
    48721 agcattgttg aaaggacttt ggagaacaat tctcctaact ttgatctcct ttgctttggc
    48781 tatagtaatc ggtgtgattt ttggattatt cagtgtagca ccaatacgcg ctcttcgcac
    48841 aattgctagt atttatgtcg atatcatccg aggaattcca atgatggttc ttgcgttctt
    48901 tattttcttt gggttgcctg gtatcgttgg atttacgatt cctgacttcc ttgctggtgt
    48961 tatcaccttg actcttaacg caagtgctta tatcgcagag attgttcgag gcgggatcaa
    49021 cgccgttcct gtcggacaaa tggaagcttc cagaagttta ggtctttctt ataatcggac
    49081 aatgcaaaaa atcattttgc cgcaagcgat acgcatcatg atcccatcat tcgtcaatca
    49141 gttcgttatc tcattaaaag acacaacgat catttccgca atcggtgtag tcgaattgct
    49201 gcaaactggt aagatcattg ttgcacgtac aacacaaagt acgtatgttt acttgatcat
    49261 tgcgatcatg tacttgattt tgatcacagc attgacaaaa ttagcaaaag ttttagaaaa
    49321 gaaggtgaag taagatggca gaaaaaatct tagttgagca tttagtcaaa aaatacggcg
    49381 ataacacagt attaaacgac atcaatgtgt ccatcaacga aggagatgtc gtctgtatca
    49441 ttggcccttc aggttcagga aaaagtacat ttttgcgttg cttgaatcgt ttagaagaac
    49501 cttctgacgg tgaaattatt attgacggcg cccacttgat ggataaaagc acagatatca
    49561 acttggtgag acaacatatc ggaatggtat tccagcactt taacctattc cctcatcttt
    49621 ctattttgga aaacatcatt ttagcaccga tggacctgaa gaaagaatct agagaagaag
    49681 cagagaaaaa agcaatcaaa ttgctgcaga cagttggtct agaagataaa aaagatatgt
    49741 atcctgaaat gctttccggt ggacaaaagc agcgtgtggc tattgctcgt gctctagcga
    49801 tgaaccctga catcatgctt tttgatgagc ctacatctgc actcgacccc gaaatggtcg
    49861 gtgatgtctt gaacgtcatg aaaaagttag cagaacaagg tatgacgatg gttatcgtta
    49921 cgcacgaaat gggcttcgct aaagaagtgg ccaatcgtgt catgttcatt gacgacggaa
    49981 actttttaga agacggaacg cctcaacaaa tctttgaaaa tccgcaaaat gaacggacaa
    50041 aagatttctt gaataaagta ttaaatattt aataaaaacc aaaaatgccc ggtcgcttca
    50101 tgacgaccgg gcatttttag tttcttgttt tttcacttaa ctgtaccagc cgcatcgctt
    50161 gttctcctgc acaaacaagc aatcgctcta cctgttccat cgcataagaa agtaccatcg
    50221 gttcattgac gatatcaagt acatagtcga taccaccttc agaaactatg atgattatct
    50281 tattctaaag ctggcttttt tgttggtaca aacatgacaa ctacaaaaaa accaggcgaa
    50341 cgtatgattg ttcgtctggt ttttaattat taatgactta gcgtttttac tagaaataga
    50401 aaatttgggc aagtctactt tatattttta ctagaaaaac tacttccgct ttttcccgcc
    50461 aaaagcatct ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc
    50521 gccagcttca gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt
    50581 gatcagtttt actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc
    50641 ttttccacgt aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac
    50701 atctccatgg acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa
    50761 aggcaattca tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg
    50821 aaaaaccaca tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc
    50881 taaacgcatt tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc
    50941 tttcttctca tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt
    51001 gccatgacaa acatcacaag tttgacggct catcactcgt ccaagtggcg tttggcgttc
    51061 tacattgatc gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt
    51121 tgcaccgttt ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc
    51181 tacaccaaaa atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg
    51241 acgaggcgca tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc
    51301 ttcaaaacca ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc
    51361 tccaccagca ccgtaatttg gatctgtgcc tgcatgcccg tattggtcgt atgcagcgcg
    51421 tttttgcggg tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc
    51481 atcaggttct ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt
    51541 tatttcatct tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt
    51601 tgccataggc tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat
    51661 attttgtttg aaacaaaaac cgatacttca aactattttt caaaagaaaa agccaaagta
    51721 gcaactttgg ccttctctcg aaattcagtg aactgattat ttatcgtcgc catcgacttc
    51781 ttcaaaatca gcatcaacga catcatctgc tccgccttga gctgcttctg gattttcttg
    51841 agcttgttgt tgtgcagctt gttcgtatag tttaacagtc aagttttgaa cgatttcgtt
    51901 caatgcatcg cgtttagttt tcatttcctc gatgttgttt gcttcaacag cggcttttaa
    51961 ttcgtcacga gctgtttctg cttttttcac ttcttcttcg tccactttgc cttctaattc
    52021 tttcaatgtt ttgtccacag agaatagcaa tgcgtctaca tcattacgta ggtcaacttc
    52081 ctctttacga gctttatctg cttcagcgtt tgcttctgca tctttcacca tacgttcgat
    52141 ttcttcgtct gttagacctg aagaagattt gatcgtgatt ttttgttctt tttgtgtacc
    52201 tagatctttc gcagatacat ttacaatccc gtttttatca atatcgaatg ttacttcgat
    52261 ttgtggaata ccacgaggag ctgctggaat atctgtcaat tggaatcttc ctagagtttt
    52321 gttgtcagca gccattgggc gttcaccttg taatacatgg atgtctactg ctggttgatt
    52381 atctgccgca gttgagaata cttgtgattt acttgttgga atcgttgtat tgcggtcaat
    52441 caatttagtg aacactccac ccattgtttc gatacctaat gacaatggtg taacgtcaag
    52501 taaaacaaca tctttcacat caccagtgat cacgccacct tggatagcag cacccatagc
    52561 aactacttca tcagggttaa ctgatttgtt tggttctttt cctgtttctt tacgtactgc
    52621 ttctacaact gcagggatac gagtagatcc accgactaaa ataacttcgt cgatttctga
    52681 ttgtgacaga cctgcatctt ttaacgcttg acgtactggt acttttgtac gttctactaa
    52741 gtcagctgtt aattcatcaa attttgcacg tgtcaatgtc atttccaaat gcaatggtcc
    52801 agattctcct gctgtgataa atggcaagct gatttgtgtg cttgatacgc cagataaatc
    52861 ttttttcgct ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt cttgagacaa
    52921 gtcaatgctg ttttcttttt tgaattctgc taccatgtag tcgatgattt tgttatcaaa
    52981 gtcgtcccca cctagatggt tgtcgccagc tgttgacaat acatcaaaga caccgtctcc
    53041 tagttcaagg atagatacgt caaaagtacc gccaccaagg tcgaatacta agattttttc
    53101 gtcgcgatct gttttatcta aaccataggc aagagcagct gcagttggtt cattgacgat
    53161 acgttctact tctagaccag cgatcttacc agcatctttt gttgcttgac gttgtgcatc
    53221 attaaagtaa gctggcacag tgataactgc tttttcgact ttttcaccta aatagtcttc
    53281 tgcgaatcct ttgatatatt gaaggatcat tgctgagatt tcttgtggtg tatatgattt
    53341 accttctaca tctactttgt aacctgcttc accgatatgg cgtttgattg atgaaatcgt
    53401 atttgggttt gtcacagctt gacgttttgc aacttcccct acttggattt ctccattttt
    53461 gaatgataca acagaaggtg ttgtacggtt tccttctggg ttagcgatga tttttgcttc
    53521 gtttccttcc aatactgcta ctgcagagtt agtcgtacct aagtcaatac cgataatttt
    53581 actcataatt aacgtctcct tctttaattg aaagtgtaag ttttatatcc gcacaattat
    53641 tgtgcaacaa tgaccatagt tggtcttaac acgcgatcat gtaatttgta gccttcttgt
    53701 agtacttcta caatcgtatc ggctggtgta tcttcagtcg ctggaacagt ttgtactgct
    53761 tgatgaagat taggatcaaa ggcttctcct ttagcaggga ttttttcaat tccttcttct
    53821 tccagtgcgt tacgcaaact ttccaagacc atttccacgc cttttttcaa acctgcccct
    53881 tgatcatctg acacttctgt ggctaatgca cgttccaggt tgtcaattga tggaagtaat
    53941 tttttggcta aatcttgtga acggtatttt tgtaattgct cacgctcatt ttttcctcga
    54001 tttgccatat ttgcgatttc agcacgagcc cgcaagtatt tatcttccat ttcttctaac
    54061 tcagctttta atttcgctgt ttcgaattct tccgcttcag cttctgaaat acccgaatct
    54121 tctgcagctt cgacatcgat ttccggttct ggctgagcat ctgtcatttc tttatctagt
    54181 tcttcttgat tttctttcac gatacagtct tcctttcatt attcagtcag ctgtttgatt
    54241 gctcttacta agaaagagag aacgatcagc tgcaaatagc ttcgttattc acattacgag
    54301 tttgacaagt caagcgagcg gtagtaatca gcaagtttag ctgctagttc tcttcgataa
    54361 acatcgacta gaccgaacat ccgagaataa ggcatactag taggccctaa caaggctatt
    54421 gtacctcttc catgaccatc tatctcgtaa cttgcttgaa tcatactcat attctgcaat
    54481 aaatcattgc ctaattcagc accaatcttg atatgaatat tgctatccat cggagtgatc
    54541 atttgtgtaa gtccgtctga gtctttcata aaagaaaaca tcgatttgaa ctgatcaaga
    54601 tcttgacggg gctcataatc caagagattc atttgtccac tgacataaac cttttcttca
    54661 aaaacatgac caagaacggt gtcaaataac tgagacatcc cttctgtcgt ctggaaatat
    54721 ttatgaagaa tgatcggaat ctccgtacga agtttatggt acaccgtcat cagaggatct
    54781 ccgactagac ggtcgtttac aatctgtacc attttttcta gttcctgact tcccatatat
    54841 tcgggaagag tgaacacttg gctttctaca ttccccatat ctgttacgac gatagcaata
    54901 atctgtcgat tattcaaggg tacgatacga aagccagtca gtcggcggtc ttttatttca
    54961 ggtcccaatg aaaaagtcgt atagcttgtc agctcagata atatctcagc agattgctta
    55021 ataatctcat tgatttcatg aaattctctg cctagagact gacgaatcag cagcaaatca
    55081 tcttctgcta cgcgagcagg ttttaacaaa tgatccacgt aataacgata gcctaaagca
    55141 gatggaatcc gcccagaaga agagtgagtc ttcaacagca atccttcatc ttctaatgct
    55201 ttcatatcat tgcgaatcgt tgctgagctg gccttgactc cctcagacat taatgttttg
    55261 gaaccaacgg gcacgccact actcgtatag tgttggatga tcagacgtaa aatgtcgttt
    55321 tgtcgctttg ttaacatctt cctcacctct cattagcact catatattct aagtgctaat
    55381 tcacttataa atatagcaag aagcccttct cctgtcaaga gaaagactcc attttttagc
    55441 actctaaaca caagagtgct aattctaaat aattgatgta aatccagtta tatcaatact
    55501 tcaatacctc attgtcaaac tttctaaact ttttcttttc aagtttcgat aaaaaacaaa
    55561 tattcttcaa actatttttc aaataaaaag cgctcaaaca cattgttccc caaaaaagtc
    55621 cctcttggtg tcaaataaac acggtcttct tcattaacaa gtaacccttc ttgctctaat
    55681 tcccttagca catcaccgta caccgattca attgtttttt gatagcgatc atagaaatgt
    55741 tttttagtaa tccctgtttt ttttcttagt cctaagaaca tttcttcttc tatttgattt
    55801 tttcgtgtca atttttcttc ttccaatacc gggcgttcat ttgctcgtaa tgggcgtaaa
    55861 tagtgttgga ttggtccttt gttgcggtaa cgaatatctc caagataacc gctagcacca
    55921 gctccaaacc caaaatagtg gtcattgttc cagtacatga ggttatgttg agattcatgt
    55981 ccggagagcc caaagttact tatttcatat tggtgacgtc catgcttttc cattgcttca
    56041 atcgtctctt caaacattct ggtttccgtt tcctgatcag gcagttcgag tcttccttgt
    56101 cgtacccagt tcatgaacat tgttttgttt tcgaggatca atgagtacaa agaatagtga
    56161 ggcagatcca aagccaaagc tttttccaaa gtatcccgat atccttctag tgtttgtccc
    56221 ggtaatgcat aaatcaaatc gatgcttaca ttggtaaaat tttctgcttc taaaaattgc
    56281 attgtttgat acacatcttc tgcagtatgt ttacgtccaa tttttttcaa taagcgattg
    56341 tcaaatgtct gcactcccat ggataaacga ttgacgccat agtttttcat cacttgaagt
    56401 ttttcacggg tcaaatcacc aggattcgct tctacagtaa actcattacc attttgaaaa
    56461 ggcaggatct ctctagcacc tgataatagt cgatcaagtt gtttagcaga taaagaagtt
    56521 ggtgttccgc ctcctacata aagtgtttcc aaattcgctg taggatgact ttccagcatt
    56581 aatctcattt cattcagcag catttccaca tactcatcta ctggctgccc ttctaaaaaa
    56641 actttattaa agtcacagta atagcagata tgctcgcaaa atgggatatg aatgtacgca
    56701 gaaaccttgt tttctgcggg atttattgtt tctatcattg aaattctttc tccttacgtc
    56761 gaaaagtcgt gcctgttttc gaccttctta aaatcgcgtt caagcaccaa ctcattgaaa
    56821 taagccgcat atttagaatc aaggaaacct agctctaaaa atgcttgctt attgtcatcg
    56881 gagctacacg gtgcttttac agcctctcga taaacggtaa tcgcaaagct gaccttcggc
    56941 attaagtcaa aatctgcaaa aatatgaaaa gctattttca cagattcctc cttaagtgct
    57001 cagaccacaa cgctttgctt acaacctctc atatacgcat actaaacgta tttggcgaac
    57061 aaatccacta aaaaatccaa gaatcgactt cttggatgat ttttttacac tcatgattct
    57121 tcctaaacaa ttttgtcctt attttattca taaaaaaatc tagtcaaatc tctcacttca
    57181 agagttttaa ctagattcca attattattt ttttgtaaaa ataaacagtc tcttaatcat
    57241 agacatttct tcggtgccct acttgaatag cgataacaac gaattgatta tcttctatct
    57301 ctaccaatac acgtatttgc caatcagagc tgcttctgaa tttcgggcgt gaattttaaa
    57361 cggcaggtca ttccagccca ccaaattcat taaggattct tccatgaata ctgttgtttt
    57421 cccacttctt atccaatgtt tatgggcaat ttcagccgtt tgacgattgt attcatcttc
    57481 tagttgttcc atcgaatagt tttttagaag ttcagataaa gtaatcccat aaaattcagc
    57541 catataagaa agccattctt ttttggcgtc acttacacga accgtaatca tagccctata
    57601 attgcctctc ttaaagaatc tcatgcattt cctgatgtga ttttagaaaa atttacttgc
    57661 taaaaaactg tgaaaatgta ggagctagca tccttgtagt aaatgatcga gctctttatt
    57721 atacattaaa aaagttcaga aattttgatg aagtagccaa ctactttttc atgagtaaag
    57781 caacactaga aaaagaatcc tccagtactt agaatttact ggaggatgta cttttgaaga
    57841 agcctatagg ttatttaaaa aatattacta caggaaggaa cgagaattac ttcgttactt
    57901 ataatagaac ttagtcttat atagaaggca tagctcacat tagcttattt gtcctataaa
    57961 aataaattac ggtattttag aaaagctatt ttctcttttt tcaacgatct tcagcaattt
    58021 ttctttatcg tcaggtaact caagttcatg aattctagca tgacaattgg gacataacgc
    58081 gatacagttt tcaatcacat cttttccacc ttgcgacaaa tattctatat gatgacaatg
    58141 taaataaggt acaccgtctt ttataaatgg ccctttttct tggcaaagtt gacaaatacc
    58201 ttttgcaagt cgtttaacat attcagatac gaagggatta cgtttatata tttttgtttt
    58261 tgagaagcaa aactcctctt cgttaacata ttttgcagcc atttttttat tataattact
    58321 aacttttctt gcttcttcct ctaatttagc attagataaa ttacgaatat gtctagtttg
    58381 attttcttcc tggttatata tctttatctt caatcaattg ctcttgattt attaaagcta
    58441 atggaaattt atatactttt ctgctgttgc ccgaactatc tttttcatta acagtatata
    58501 tttgtcctgc taaatatact tctcccagat atgtgtgttc ttttggttta aatgtttcaa
    58561 ataaatgaat ctttacaaaa ttactttttg aattggctaa aactttattt tgattcttat
    58621 ccaatacttg atcccctttt agccccatac cggtatattg taaaatacca tttaaccact
    58681 tatctttata aacattactt cctgtttgat cactaaacag tacaagagta ttagtttgat
    58741 gagatcttct cattccaccc tgtttggaac atttaaactt ctctgaaatt tctttgttag
    58801 tatatactct accaacttct aactcaccta atatgttttt agaccaattt tctcgccatg
    58861 taataatctt atcaagatta aacatttctc cctcttttgg catgccattt ctaattaaat
    58921 tagctaatac accagtagaa atatctaatt tttttagtaa ttctttctta gtaatcaatg
    58981 ttggattcat aacaatgcag ctcctaatga tatcaatata attgcattag ataacttgaa
    59041 aaatcgaaaa aaactcattt tgtgtcaagt tatctgtaat atataaaaat atcattatta
    59101 tacctcatct atgtgaattt ttactattct taccttagca tattctttat aggtatatca
    59161 gagctaatca aactaaaaaa tacccgaagt cttcttatgt aaaaacaccg gttattactt
    59221 aaatatgact taggtaaaac aatgagtgat ccaattataa aattttatgg cgaataattt
    59281 agtaagcgct acatattgaa tgatttagaa attttgaaaa agtagctagt tccttttata
    59341 ccagaaaagt aaacctaata aaaaacacaa ctaattttta gctgtgtttt ttaagttaca
    59401 ataattatct atttaatagt cttaattcat taaaataaaa aactcaactt tacaaggata
    59461 tggtttaaaa aatttattta tccaacccaa ctagttttcg ctgcttacga tttgcttcat
    59521 ctgctacgta attcattact gttttaaatt ctttcgctga ggcgttttct gataaatcag
    59581 cgatactttt attatacttc ttcacgattt gttgtacttg ttctttctca gtcatgtaat
    59641 tcctcctcca actcttttga agtatattgt attggtagta ctaattgaat attatctgct
    59701 gcataatgca cttcagaaat gtcgttttga taatccttaa tcattatgtg aaggactact
    59761 tcaggatgtc tatttttgac tttttcaata ttctctctgg acttaaaaaa cgcattgata
    59821 aacatttctt ttggcacatg acgtccttca gctagttcac gttttcgagt gaaatcccag
    59881 gcaactttgg gatcttggta aacatagaaa atcgtaacgc gaaagttatt tttaagggct
    59941 cgaataatat ttttctctgc actaaggata gcaaaagtat cctctagtat gaaagaatag
    60001 ccatgttcca gtacatattt taatgattgt tcaactaacc aagaagaagc tttctgaaaa
    60061 acacttgagt tctttccatt gtattctgga aattgtgctc taaaatagtc cgcatcaatc
    60121 acaacatggt tgtcataatt ttaagctaaa cctagtgcaa cctctgtttt acctgctcct
    60181 gggcttccag ccatgaatac cgcatctttt tcatcatcag ggatttttcc cttgatgatt
    60241 gtttcaataa atttatcttt attgacccta gcgtaatcta aatacttgtc tttaggttcc
    60301 aagattgcac cttctttcct tcttctatat tcatactaat tatacgatat ttatataaaa
    60361 aatataacgg tcattttaac catattaaca tttagtcaca gtaatagcag atatgctcgc
    60421 aaaatgagat atgaatgtac gcagaatcct tgttttctac ggcattcgct tttttttcat
    60481 tggactagtt atatatttct agggttgttt tcatgtctga gtgcccgata tgatttattt
    60541 ttcgtttcta taattacttt ttgccttcca gtttctttta agattgtact caagcaagtg
    60601 ggaattggtt cttttgtcat ccatgtatgc aaaatgttat aatgctggta aacaaaaatg
    60661 attacctgtc atttttattt agttcgtatt taattaagtt gactgtctgt atactctgtc
    60721 taaaacttaa gaattgttgt tttcgctgtc gctatccaaa tttaatgaat gtctgtatgt
    60781 taaaactgtt gtccttgtca tagatagtaa aggagagctt gaaaatgaaa actgcgatta
    60841 cgtttctatt agaggaatat gccgattggg aaggtgctta tattactagc acactaaacc
    60901 aaaaagaggg atggcaaaac aaaactgctt ctatccaatc agaggtcact tccattggag
    60961 gattaaaaac aaaaatcgat tacttaatcg aacaaatacc tgaaaaagtt gaccttttta
    61021 ttatgatcgg tggcaattca tgggacataa aaaatcatca tttatatcaa ataatcgata
    61081 aattgttaaa aacagacgta ccagtagctg ctatttgtgg ggcagttgat tatttagctc
    61141 agagcggatt attgcaaggt tacaaacaca ccggaaatgc gcaatttcct tggttagaat
    61201 cggaaaatta tcagaataaa gaagatttta tagaacaaca agctgtgaaa gatcgtaatt
    61261 tgattactgc gaatggaaca gctcctatag aattcacaga cttgattctt caagcagtcc
    61321 attttgaaac agaagaaata agagaaaaaa agattcgtct atacaaactt ggattctatg
    61381 attactataa aaagtacgga gatcctttct cctaatcatt ctcttcaatt aaatactcct
    61441 tatcaatagt atgacaagta attttactga tggttatttg tcacactttt tctgacattt
    61501 atcaaagaag atacactcta tttatattca tagatggaat ggcaacagtt tttttgacaa
    61561 attttataag gtgcagaact tctttccgta tgctattccg attgtccttg acaatgagcc
    61621 tcctcggatg tgacgatctt cggcaggagc taacagttaa agttagactg cctcgctagc
    61681 gttagcacat ccgagctcat tatcaaggat tcgctgc
//
LOCUS       DACDYM010000005        57516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDYM010000005 DACDYM010000000
VERSION     DACDYM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.005"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IWM59_000353"
     CDS             complement(61..2424)
                     /locus_tag="IWM59_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP9978414.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="IWM59_000354"
     CDS             2872..3093
                     /locus_tag="IWM59_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9978415.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IWM59_000355"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IWM59_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9978416.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IWM59_000356"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IWM59_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9978417.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="IWM59_000357"
     CDS             complement(5894..6205)
                     /locus_tag="IWM59_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9978418.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="IWM59_000358"
     CDS             complement(6202..6495)
                     /locus_tag="IWM59_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9978419.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IWM59_000359"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IWM59_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9978420.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IWM59_000360"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IWM59_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9978421.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="IWM59_000361"
     CDS             complement(8333..12685)
                     /locus_tag="IWM59_000361"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9978422.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="IWM59_000362"
     CDS             complement(12897..14606)
                     /locus_tag="IWM59_000362"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9978423.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IWM59_000363"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IWM59_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9978424.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="IWM59_000364"
     CDS             complement(16103..16903)
                     /locus_tag="IWM59_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9978425.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="IWM59_000365"
     CDS             complement(16900..17712)
                     /locus_tag="IWM59_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9978426.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IWM59_000366"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IWM59_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9978427.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="IWM59_000367"
     CDS             complement(18467..19189)
                     /locus_tag="IWM59_000367"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9978428.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="IWM59_000368"
     CDS             complement(19325..20206)
                     /locus_tag="IWM59_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9978429.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IWM59_000369"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IWM59_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9978430.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="IWM59_000370"
     CDS             21446..21925
                     /locus_tag="IWM59_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9978431.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="IWM59_000371"
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="IWM59_000371"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9978432.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23226..24011
                     /locus_tag="IWM59_000372"
     CDS             23226..24011
                     /locus_tag="IWM59_000372"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9978433.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="IWM59_000373"
     CDS             complement(24357..26048)
                     /locus_tag="IWM59_000373"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9978434.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IWM59_000374"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IWM59_000374"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9978435.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IWM59_000375"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IWM59_000375"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9978436.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IWM59_000376"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IWM59_000376"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9978437.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            30332..31033
                     /locus_tag="IWM59_000377"
     CDS             30332..31033
                     /locus_tag="IWM59_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9978438.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="IWM59_000378"
     CDS             complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="IWM59_000378"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9978439.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="IWM59_000379"
     CDS             complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="IWM59_000379"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9978440.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="IWM59_000380"
     CDS             complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="IWM59_000380"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9978441.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(33615..34439)
                     /gene="tsaB"
                     /locus_tag="IWM59_000381"
     CDS             complement(33615..34439)
                     /gene="tsaB"
                     /locus_tag="IWM59_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9978442.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(34528..35508)
                     /locus_tag="IWM59_000382"
     CDS             complement(34528..35508)
                     /locus_tag="IWM59_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9978443.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(35532..37016)
                     /gene="galT"
                     /locus_tag="IWM59_000383"
     CDS             complement(35532..37016)
                     /gene="galT"
                     /locus_tag="IWM59_000383"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9978444.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(37028..38017)
                     /gene="galE"
                     /locus_tag="IWM59_000384"
     CDS             complement(37028..38017)
                     /gene="galE"
                     /locus_tag="IWM59_000384"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9978445.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            38266..38433
                     /locus_tag="IWM59_000385"
     CDS             38266..38433
                     /locus_tag="IWM59_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9978446.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(38495..40306)
                     /locus_tag="IWM59_000386"
     CDS             complement(38495..40306)
                     /locus_tag="IWM59_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9978447.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(40303..40668)
                     /locus_tag="IWM59_000387"
     CDS             complement(40303..40668)
                     /locus_tag="IWM59_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9978448.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(40831..41226)
                     /locus_tag="IWM59_000388"
     CDS             complement(40831..41226)
                     /locus_tag="IWM59_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9978449.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(41244..42206)
                     /locus_tag="IWM59_000389"
     CDS             complement(41244..42206)
                     /locus_tag="IWM59_000389"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9978450.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(42206..42418)
                     /locus_tag="IWM59_000390"
     CDS             complement(42206..42418)
                     /locus_tag="IWM59_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9978451.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(42439..43137)
                     /locus_tag="IWM59_000391"
     CDS             complement(42439..43137)
                     /locus_tag="IWM59_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9978452.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(43157..43699)
                     /locus_tag="IWM59_000392"
     CDS             complement(43157..43699)
                     /locus_tag="IWM59_000392"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9978453.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(43831..44835)
                     /locus_tag="IWM59_000393"
     CDS             complement(43831..44835)
                     /locus_tag="IWM59_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9978454.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(44832..45821)
                     /locus_tag="IWM59_000394"
     CDS             complement(44832..45821)
                     /locus_tag="IWM59_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9978455.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(45818..46624)
                     /locus_tag="IWM59_000395"
     CDS             complement(45818..46624)
                     /locus_tag="IWM59_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978456.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            46790..47746
                     /locus_tag="IWM59_000396"
     CDS             46790..47746
                     /locus_tag="IWM59_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9978457.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(47823..48341)
                     /locus_tag="IWM59_000397"
     CDS             complement(47823..48341)
                     /locus_tag="IWM59_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9978458.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="IWM59_000398"
     CDS             complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="IWM59_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9978459.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            48806..49252
                     /locus_tag="IWM59_000399"
     CDS             48806..49252
                     /locus_tag="IWM59_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9978460.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="IWM59_000400"
     CDS             complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="IWM59_000400"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9978461.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(51667..52641)
                     /locus_tag="IWM59_000401"
     CDS             complement(51667..52641)
                     /locus_tag="IWM59_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9978462.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(52977..53064)
                     /locus_tag="IWM59_000402"
     tRNA            complement(52977..53064)
                     /locus_tag="IWM59_000402"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53030..53032),aa:Ser,seq:cga)
     gene            complement(53209..53775)
                     /locus_tag="IWM59_000403"
     CDS             complement(53209..53775)
                     /locus_tag="IWM59_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9978463.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            54030..55361
                     /locus_tag="IWM59_000404"
     CDS             54030..55361
                     /locus_tag="IWM59_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9978464.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            55327..55677
                     /locus_tag="IWM59_000405"
     CDS             55327..55677
                     /locus_tag="IWM59_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978465.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(55769..56188)
                     /locus_tag="IWM59_000406"
     CDS             complement(55769..56188)
                     /locus_tag="IWM59_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9978466.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(56189..56533)
                     /locus_tag="IWM59_000407"
     CDS             complement(56189..56533)
                     /locus_tag="IWM59_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9978467.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            56883..57479
                     /locus_tag="IWM59_000408"
     CDS             56883..57479
                     /locus_tag="IWM59_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9978468.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT        16181 a        12117 c        10052 g        19166 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgga acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt
    30121 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
    30181 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
    30241 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
    30301 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
    30361 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
    30421 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
    30481 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
    30541 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
    30601 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
    30661 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
    30721 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
    30781 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
    30841 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
    30901 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
    30961 cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
    31021 tatgagagaa taacgcgaac aaaaataagc agtgaccctg atatttatca gggtcactgc
    31081 ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg ggcaagactc
    31141 aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgata
    31201 gattaactag aggaagcgtc ttgtcctcat ccgacctaga ttattcgata ttcctcgaca
    31261 ataaacgatt aatcagtgag ggcaatcaga ggaacggata atgatgaata agctgtaaaa
    31321 aatagggctt tgacaaaagc gtgtcacagc ccttatttcg attaaacggt gtgtacctgt
    31381 caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt
    31441 tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa
    31501 gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg
    31561 gctgttctta ttacttgatg cagggcagat ttttcacaac ctctttattc agataaaacc
    31621 aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt
    31681 gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt
    31741 tctacctcag ggagtttagt tgccaaagca tcctgcaatc cttcacgcaa cccttgattg
    31801 gccgctacac ctccagctac aatcagttgc ttgactggat aattttgaca agctcgaagt
    31861 gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg
    31921 tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat
    31981 ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgtggaaa atggtaatta
    32041 tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat
    32101 actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt
    32161 tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt
    32221 gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata
    32281 tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata
    32341 ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct
    32401 tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg
    32461 tgatggcggc tagctacttc aggaactacg ccaccaaaac gtttatgact gtttatttga
    32521 gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca
    32581 tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta
    32641 actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac
    32701 ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta
    32761 cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat
    32821 gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt
    32881 catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca actaaaagca
    32941 gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac
    33001 catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa
    33061 aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca
    33121 acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaaaccta
    33181 acttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct
    33241 cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt
    33301 tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc
    33361 ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa
    33421 gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga
    33481 cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt
    33541 aacgacgatc gatttgttcg attgtttttt ctgtataaat cattttatct tgaaaaaagc
    33601 ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc
    33661 aaccattttt cttctgcttc tacccgtttt aaatagcgag gaagaaaagc ttgtttctct
    33721 gcaactggtt cagctaaagc acctaattga gccacagtta caccacttgg aatatcccat
    33781 gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcata gaagaatttg
    33841 tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa
    33901 tcagaagcag aaatatgtac atcttcaagt acaatttcta atctattttc tttccagcgg
    33961 tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aattttatcc
    34021 acaccgatgc aattagccgc gatggttttc agactagata ctcctactag ctctttatct
    34081 aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg
    34141 ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac
    34201 aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt
    34261 tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct
    34321 attcctaatg taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta
    34381 gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca atgatacatg
    34441 attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac
    34501 ttcatttccc cacagctttt ccatctttta tttcgttgat tctcgaataa tcaactctgt
    34561 ttccacaata atctttcttg aaaccggtgc tttggaatta cttaattgaa gaatcgtctc
    34621 cactgctagc tctcccatcc attcggtata aacttttaca gtagaaagag agggcgttac
    34681 atatttcgct acactgacat cattgaaacc catcacagaa atatcttctg gtaccactaa
    34741 acctttttct tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag
    34801 cgcatcagga acatgcggat tcgtttttaa atactgcttc actgcttgat atcctgactc
    34861 gacagaaaaa tctgcttcga taattggacc ttcgtcgtag cctttttctg ccaaaacttg
    34921 ttgaaatgcc ataattctgg gatcttttag ccgctgatgg tttttcttcg tatattctgt
    34981 tccagcaagc atagcgatat gttgcgcttt atgggagaca aaatgttcaa tgactttttg
    35041 aacacttgac ttaaagtcga caactagaga gtcgattcct tgagaagtgc cgtcagaatc
    35101 taccaaaagc aaggcatttt gggatccttt catttctaat aactgagctt cgtcgaattt
    35161 tcctagtgcg atcgttccat ctgtcggaag atcactaatg gaatcccacg tttctttcaa
    35221 taaagtaacg tttgcttctt ctgccttttt ttcgattcct agacgaatag ataaataata
    35281 aagatcttca agctcttccg tatcgtcgta ccactggatc aggcgaagcg tttgcttagt
    35341 atttgctgca tttttacgat gtttcgtata atttaagcgt tctgctgctt caaaaatttt
    35401 ttgctttgtg gctaagccaa cggaaagatc aggatcatag ttgagaactc ttgaaacagt
    35461 agcaggagaa actcctgcca actgcgcaat gtctttaatt gttgccattt gtctctcctc
    35521 cttttttacg tttataaaat tgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct
    35581 cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac agatttttgt
    35641 acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc
    35701 cacggacgat gatattcagc catctggttt tcttctccta ataagaaatg ctctacttct
    35761 actagttctg gtttcaaacg aggtggtaaa acagcaagcc ccattacttc gatcaaccca
    35821 atgttctcct ttttgatatg ttggacatct ttatgtggat gaaaaatccc atcaggatgt
    35881 tcttctgaaa cattgttgtc tcgaaggaca aggtccaact caaagtaatt gtcccttctt
    35941 cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc tctgatagat
    36001 actgtttcat ccgaatatgc tttccacttt tccaatactg agttagctgc ttcaatcaac
    36061 aattttttct ctgttgattg caaacggatc acagacatcg gccatttcac gactcctgcg
    36121 atgacttgcg gataatctgc tagtttgata agtttttcta tcggtgcttt tgccatagga
    36181 aattcatgac gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc aacaatcggc
    36241 aagtcagcat tcgaaccaac aaagtaatga ggcaagactt cagtaatccg aagcagtcta
    36301 gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt
    36361 tcattgtaat aagcataagg agaatattgg aatccccagc tttcaccgtc tagattcatt
    36421 cgaataatcc ggtgattggt gcgggctgga tagttcaagc gacctctata tccttcgttt
    36481 tccatgcaaa gcatgcaggt aggatagtct acttttaccg cctcgcgttc tgctgcgatt
    36541 tgttttggat ctttttctgg cttagataaa ttgattgtga tttccagttc accatactcc
    36601 gactctgccg gaaatttgat gttttttgcg atggctcttg ttttgatgta gtcatttttc
    36661 ttacttaaat taaagaaata ctcagttgct tcctctgggc ttttcgaata gtgctgtgca
    36721 aagaatgcat tgacgactga aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa
    36781 atctcttgtt ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt agcgacaagc
    36841 tgatccaata agtctacaga ttcttcctta actggtctta cttcaatgct ttctagagca
    36901 tcttctccta ccatccctaa aagacgattc tgtaaatata agcggtccat ttccatccat
    36961 ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat tgacatatct
    37021 cattccccta ttcttcataa ccatttggat ggctgacatg ccaatcccat gctgttttga
    37081 taattgcttt gatatctgtg tattcaggtt tccaacccag aacctctctc gctttttcac
    37141 ttgaagcgac taatcggcta ggatctccag ctcttcttgg tgctatttct gctggaattt
    37201 cttttcctgt cacttctcta gctgcttcta gcatttcctt gactgagtat cctttgctgc
    37261 tgcctaaatt gaagaagttg ctttcatttc cttctttcaa gtattccaaa gctaaaatat
    37321 gtgcagcaat caaatcttct acttgaacat aatctctgat acatgttcca tctggtgtat
    37381 cataatcgtc tccataaaca gccaatgctt ttctttgacc aagagctact tgtaaaatga
    37441 ttgggacaag atgcgtttct ggtgtatgat cttctccgat agatgcatct gcttttgctc
    37501 cggctacatt gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg tcacaccact
    37561 tcatcatttt ttccatcatc aatttacttt caccatacgg atttttagga ttagctggtg
    37621 tatcttctgt gattggagac tcttttggtt ctccgtaagt tgctgcagta gaagagaaca
    37681 cgattttatt cacattgaac tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt
    37741 tgttgttgaa atacatcaat ggtttttcga cagattctcc tactaaagag cttgcagcaa
    37801 aatggattac tccttcaatc ggttcctttt caaatacgga acgtagaaat tctttatccc
    37861 ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga cctgtgagca
    37921 aattatctac cacagctact tgatacccct tttgaactag ctggtctaca gcgtgcgaac
    37981 cgatataacc ggctccgcct agtactaaaa ttgtcataaa aaacactcct ttgattagta
    38041 aaattgtgtt tgttgattat atataattta aaaaaacgaa gtttactttt atggcgaaaa
    38101 caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac
    38161 attttcaagg attttttgca aaggtttaca aacaaacgta aaactagcct ttttttctct
    38221 tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa aaaatttgta
    38281 tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt ggtcgcttct
    38341 gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga agaaaaccgt
    38401 aaaaaagcaa tgcgcaaacg cgtttctcgt taataattat ttaaaaacac gcatgaagac
    38461 ttgtgaaaga acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg
    38521 ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag
    38581 ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc
    38641 ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac
    38701 ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta
    38761 tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc
    38821 gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat
    38881 caaatggcgg ttcgaacaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg
    38941 tccgaatgac tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata
    39001 gtatttcttt gtattccttt gcagaaaaaa cgtgccattc aatagcctgc cggattggat
    39061 cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt
    39121 ccgcttcttt tatctgccct tcaataaatg ctcggctagc ttctgcaaag gactcatcct
    39181 gataggtaag aggaatttct aaatacgacc aatcccatct ataagggatt agtgctggat
    39241 gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg
    39301 gaatcggcaa atgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt
    39361 tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt
    39421 tttcttttgt tagtaaccgt ggccatgcca ttgatccgcc ttgcttttgg tttcttcctc
    39481 gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg
    39541 gatgatagac aagttttcct ttttcgttct cgaagcgccc ttctcttgat actgtaaaca
    39601 atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg
    39661 gtatcttcga aagatcgaca tctgcaggat tagaaggaag ctgatacagt aaatcatttt
    39721 tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc
    39781 ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat
    39841 cttttctttt gctgactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt
    39901 cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt
    39961 gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg
    40021 tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg
    40081 ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag
    40141 aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg
    40201 gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg
    40261 ctatacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat
    40321 ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac
    40381 aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt
    40441 ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg
    40501 tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc
    40561 taactgcgct ttttcttttg aaattcctcg tggccagaac ctatccacga gtattctata
    40621 gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt
    40681 ctttctgcct tttcgcttag cttatatatg tagataatag cacaaaataa aaaaaatgaa
    40741 cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat gattgattcg
    40801 gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga tttttccaga
    40861 ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt tacgtaattt
    40921 atttgctgta cggatgctca agcttttctt ttgatcgtaa atatagacag gctggtcttt
    40981 tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat
    41041 atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt ttctcattcc
    41101 ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgc ttggccatta ctcgtagata
    41161 gacttcccaa aagatgattg caagcacaat caatagcaaa atgatattaa ttacccataa
    41221 aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc agcaccaatg
    41281 acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct tacttgcggg
    41341 aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc tgctgccgat
    41401 actcctccac ctaacacaat acttgacgga ttcaatgtat tccccaagtt ggcgcaagct
    41461 aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc gtcttcttga
    41521 gccatttcaa agatatcttt acttgttact tcttcaccgt tgtctaacat tgctttcagt
    41581 tttgaatctc ctgcgtattc ttctgccaag taacgaccta gacgtaccac accagtagcg
    41641 ctagacacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc
    41701 acagtgatat ggccgatctc tccgcctgct ccgctcacgc cgtgaatcaa atttccgcca
    41761 gcaacgatac caccgccaac acctgttcct agagtaacga atacaacgtc tggatcgttt
    41821 tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcatcatt atcgatagcg
    41881 aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc tgtccagttt
    41941 aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg ggtacccatg
    42001 ccgattccga taaagtcttc ggctttcata ttatataaat tcaaacgatg gttgattgat
    42061 tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc gatgctccat
    42121 ttttgttgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa
    42181 tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt tcttcaattc
    42241 ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca atcaaacggc
    42301 tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg cgtgcaccca
    42361 catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag ctctccatct
    42421 ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca gtaaaataca
    42481 tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta tcgaatagct
    42541 tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa ttagcccacc
    42601 gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg tcaacacaat
    42661 aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc
    42721 taagataaac aacgcaccga acaaaccgaa tatcgaagta ctcgcaccac cagacagaac
    42781 attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga acatataaat
    42841 caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa aatacagtat
    42901 cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg tcactaaacg
    42961 ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat aagggatctc
    43021 aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata
    43081 ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat aattcatgaa
    43141 aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca aagctctccg
    43201 gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt ttatgaaaat
    43261 tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg gctctattgt
    43321 agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc ttctctgatt
    43381 gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc atttcataaa
    43441 acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt tctttttctg
    43501 ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc gtccctatca
    43561 cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc tgttcttttt
    43621 cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc cggcgtaatc
    43681 ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt ataggaaaaa
    43741 aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa gaagacctag
    43801 aaatgattgg ccttcctttt ttaataaatt tcaactgttc agcgcgtgtt tttgtctgat
    43861 aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc ctgtaggtac
    43921 ttccccactt ggtagaatca ctctagcaac catatctgca ctaagcagaa aaatggaacc
    43981 gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta gcttcgcagc
    44041 atgaggagct agtagtccta caaaagataa actacctgtc accgctacac taccgcttgc
    44101 caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa ggcctaatcc
    44161 ttgcgcgcgt tcttgaccaa atgatagcaa tccaatcctc tgtgcataaa gtcctcctag
    44221 cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc tcgcacccca
    44281 gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata attggatcgt
    44341 gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca ataagattcg
    44401 attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc aagcagctaa
    44461 aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt gcgaaaagaa
    44521 acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata tactagaatc
    44581 tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa gacaagctcc
    44641 tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca gcgccaaaga
    44701 agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc
    44761 aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat agaacgtcca
    44821 atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg aacgccaatc
    44881 aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct cgctgtaaaa
    44941 tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac ataacgatag
    45001 tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa tgaaacaggt
    45061 ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac aatcaaccgc
    45121 gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg aatcaaagat
    45181 attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg aaacaatact
    45241 gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc aagatcctgc
    45301 tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga actaatagct
    45361 acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg caaaaaacca
    45421 aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac agaaaaatca
    45481 gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc cgcgttgatt
    45541 cctagtaagc ctgaatctgc taaaggattt cttgtcactc cttgcatcaa tgcaccgctc
    45601 aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg aatatttcgt
    45661 atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca aaaatctgct
    45721 tttcccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa
    45781 cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc tcctgctctt
    45841 ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc gtgacaatcg
    45901 ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct tcaggtgtac
    45961 cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa cgtgaagcat
    46021 gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg atttcagtca
    46081 ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga
    46141 tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg gtttgtccac
    46201 cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca tccaacgccc
    46261 actcgattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga taagggtatc
    46321 gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta gtttgtgcta
    46381 ataatgtaat tttttttgca agttcttttg aactgtatgt agagatatca gagttattga
    46441 caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat aaagtagatt
    46501 ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc gtaacagaca
    46561 tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct cttaatctcg
    46621 tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc
    46681 gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt cgaaatatca
    46741 ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa tgaaattaaa
    46801 aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga gcgcttgctc
    46861 cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat ccaatagtac
    46921 tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc ccaaacggat
    46981 catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta tcgcccagtg
    47041 gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg atgtcccaac
    47101 gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt tgctgttgat
    47161 ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac
    47221 ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag atgtcggcaa
    47281 agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa aatccatcaa
    47341 gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag tgttgtgggt
    47401 gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa tcgtatacga
    47461 cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag ccacttctga
    47521 ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca tcatccttgt
    47581 gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc ttaaagcagt
    47641 gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata acggacctat
    47701 tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt aactaaaaaa
    47761 agaatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta gacgcataga
    47821 ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg aaaacgatgg
    47881 gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc gaagtctgaa
    47941 aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag
    48001 atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga aacatagata
    48061 tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat tacttccgca
    48121 ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg ataaggaagt
    48181 gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg atccattgta
    48241 tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc atttttgccg
    48301 atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc tccttatgat
    48361 tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa ttgagaactt
    48421 taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt
    48481 tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata gttacgttct
    48541 ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc catttttaca
    48601 agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt ctagattgct
    48661 agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac ggttttactt
    48721 ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat atagattatg
    48781 ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc ctgcatcaat
    48841 gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg cattattaga
    48901 tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa aaaaatggta
    48961 tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac
    49021 ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga
    49081 atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt
    49141 tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac
    49201 tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt gaacatatgt
    49261 aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct
    49321 ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg ttttttgaaa
    49381 ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat tgatatttga
    49441 ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt catctacaaa
    49501 gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt gctcttcttt
    49561 acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa aaaattcttt
    49621 tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt ctttcaacca
    49681 agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt ccggaagatg
    49741 tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata aaaacttact
    49801 tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc ggacttccaa
    49861 agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca tccattgatg
    49921 aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt tctcgtaatg
    49981 aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta acacttctga
    50041 tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg caggagcaag
    50101 tgattcatat aaatggatcc gtccatggaa atttttcatt tttccgattt tacgtcctaa
    50161 attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac ggatgaattt
    50221 tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg ccccttctcc
    50281 agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca aaggtacgca
    50341 agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg ctttgaacga
    50401 catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg ttagttttct
    50461 ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag aatgatctgg
    50521 tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag aaccaacttc
    50581 tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga ttttttcgat
    50641 ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat attgctgaat
    50701 gtctagtttt ccatctttat acgtataata atggccttca ggcaaacgat agacatcttt
    50761 gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt tcaacgcttc
    50821 tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa
    50881 cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt ggtctcttgc
    50941 accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc cgcggatctt
    51001 ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg tatctgcatg
    51061 tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt aattatagat
    51121 ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct gagtaccgcc
    51181 ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat cgatatattc
    51241 tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa taattgtttt
    51301 tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt cacatcctaa
    51361 tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat agcggacgaa
    51421 tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca
    51481 gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta ccttattctc
    51541 ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaacagcaaa aaaagacacg
    51601 acctaaagat cgtgtctaat tatgactgta tcagaaaata tgataccaaa cggatctatt
    51661 cattaattaa ttgacatagt ttatctgttg ctcttttatg actggataac tgaccaattc
    51721 atgacgatcc aagtcttctt tatttatatt tacagcagta atttgactgt cttcatacgt
    51781 acgataataa taaatccctt tatcaacgtt acagcaagaa gaataaatcg tatattcgta
    51841 ttttccttct ccagtatcgc atactccttt ttgttgctcg actgagccta gtatatgaaa
    51901 aaattgactg atactttcag attctgaatc acctgatgca gcattcattt tagtaaatgt
    51961 ggcttttaca aatcgagaaa cagaagagag atctccaggt aagcccaatc ctcccatccc
    52021 acgactataa acattcaagc tgatctggtt tgaaaaatga ttttctggtg ttccatttga
    52081 tagcgagcga taattgttca gattaaataa ttgataatca aatggcggat tatttgtcaa
    52141 cacaccgaca ggattatcat ataaatgaag tccatctttc atactttcaa ttactatcga
    52201 tttttcttta tcagctaata accagtgtaa aggtgacaag ggtagttttt cactaaaatc
    52261 gatattgact agattgatag tagccaataa ttctttagct tcgtctatgg ttgagcattg
    52321 ccctagtatc catggaataa actcaaatgg tgcaacgtta tcttttcctt cttgtaattc
    52381 tttataatca gcattgccag gaaaattcag tcctgccata ctcaaccctt tttcatttgt
    52441 agcctcataa tagagtggat agttggatac cccagctgct attcctatca ttgcataatg
    52501 tttatctaaa ttttttacct ttctaaaatc gaaacgataa tttcttggag taatagtgac
    52561 tacttcatta taagacatct cataatcaaa attccttcca aaataatggt ctttcgttgc
    52621 ataagtgatt gcggtacaca tcgttttccc tcctaaaaca aatcgttttt acacttttat
    52681 cttacatcta gtattttttt ttatcaaaaa aaaggccaaa gaagattgat ttactagcat
    52741 ttataaaaaa tagtactcag ggggaatcaa aaaatattgt aggttaaaag tacaacaata
    52801 aggggctgtg acaaaagtct tgtcacagcc ccttattccg aacaaacggt gttcaaaagc
    52861 ttgcatctgc ggtaataagc ccaaccaaac gaaaaaatag gaaaagctat ttttgtttgg
    52921 ccgttcttat tacttgatgc aggacagctt cttcacaacc tctaatcgtt gatataagga
    52981 aggtacagga tttgaacctg cgcgccggta ttagccggtt cgccggattt cgagtccggt
    53041 gcattaccac tctgccaacc ttccgtaagt actattttta aacttttttc acgaaatgtc
    53101 aagcctattt ctaatagata aataacagaa atcgcttttt ttagcaaaaa agtccattga
    53161 cagtttgcag ccaacggact taccgatcaa attcatactg atagaacact atctgattat
    53221 ttcaggatta tctaatgcta aaatcgagtc aattgtccgt ttgatcagtg caatgatttg
    53281 ttcttctgtt ggatgcatga tgtttgatga catcaatgac gctaagccag tcactgctac
    53341 ccatgtacca gtatgcagag catcgacata ttcatccgat aattgattgt atctttcatc
    53401 ttctttaact atttcacgga aataattgta tgaaaaatca tgcattttct ttccgtcccc
    53461 atgttcatca atgtacaatg ccatgtaaag actatgatgc ttttttgcaa agtagatata
    53521 gttcaaaccg agatcaatga ttttatcacc agtacgttca atagggaaaa catcttcttt
    53581 tagttgttca aagatagaat ccagcaacgt attttttaaa tcttccatgt ttttgaattc
    53641 cagataaatc ggctgtgtcg aaatccccat ctttttagca atatttctcg cagtaaatcc
    53701 tttaaaccct tcctttgcaa caacatcata agcagctttt agaatttgat ccttggtata
    53761 gacttttctt ctcattaaaa ctcccccact tctttactgc agtatatgtt gttttattgt
    53821 accctatttt tagcattttt tgcaaacatt ttactcaaaa cgttatattt taaatttttt
    53881 tcagtcttct ttaacttttt tttgcaaaat taaaaaaatt tggttaaatt caatgggttc
    53941 ccttacattt ttagttttga cctaaatgat agttgtccaa atctttcatt tcaagttata
    54001 gttaaagaaa atgaaaaaga aggtgtgtaa tggaaaacga aactgacgtt atttatattc
    54061 atcctcaaaa acgaatcgtt tcacagaaaa gaaagtattt ttatcttggt tttacaggtg
    54121 ttttttttct ttttattggc ttgctttcta atactcctac tgataattgg agtggattgc
    54181 taactattct tacctcgcca agcaatcttt tgacagatta ttttgcttta ggtgttttcg
    54241 gaagcgcatt cataaatgta gggattttga ccttactttc tgtgctttta gcttatcggc
    54301 acaaggtgat tttaaacggt cccttatttg cttcgattct aacagttacc ggtttctctt
    54361 tttttggcaa aaacctttat aattctatct caatcatttt aggtgtgtat ctatacgctg
    54421 tatttgtcaa taaacctttt tctcagtaca tcatgatcgg actgtttggt agcgtattat
    54481 caccggtcgt tagttatatc acttttggta tgcgttttcc tttactgata ggtattttat
    54541 tggggaattt agctggcata gcaattggta tacttcttcc gcccttagct gctcaaactt
    54601 tagttttcca tcgtggattc acactttata atattggatt cacctctggg ttgattgcga
    54661 tgacttttac tgctgttttg aggttattca gctattcaat cgtcgaaaat acattggttt
    54721 tcaatgaata tcactttcct ctcatttgga ttatttttgg ctttttttcg ctgacagtcg
    54781 gaattggttt ctattataat tcctttcggc tttcggggat tcgagaaatt ttcgattcat
    54841 ctggaaaatt gacaactgat ttcattgcta attctggtat tggtgctact ttgatcaaca
    54901 tgggcttagt tggtctgatg ctgtcttctt atgttttgct ggttggcggt cagttaaatg
    54961 gacctgtcat cggtgctatt ttatctgcgg tgggtttcag tgcttttggt tgtcatttaa
    55021 aaaacagttt tccgatacta gtcggaatat ttattgcttc tttgtttgga acttttcatg
    55081 agatcaccag tactggcatg cttgttgcag ctgtgtttgg gacaggtctt gcgcccataa
    55141 gcggttttta cggcagtttt tacggtgtaa tagctggtgt gctacatatt gctcttgtcc
    55201 ataacgtcag cactttgcac ggaggactaa atctatataa cagtggtttt tcaactggtt
    55261 tcgttgcagg tatccttgta ccgatactag ataactttac cgctgtcaga aaggaaaaaa
    55321 agacacttgg aaaaagaatt atcaaaaaga atcatcgatg agcttcttac ttatttatat
    55381 tcccatgaaa taactgaaat acgtattgga gtagaatttg ctgaggaagg gcttttcata
    55441 gagatacagg gtacggcaga aactcagcca gaagatttag atgtacttgc tgatttgctg
    55501 aacaatcctc gggaccctag tatggaaatc tactatcaag aactattagg taatccccga
    55561 catgatcggg aagattatca tctgttaggt agtttgattg atgacgtgga aatcatgtat
    55621 gcccagccga ttatcaatat taagatcttt cgacgtgctt ctcattccta ttattaaccc
    55681 tattttctat ttataaaaga ctgaatcaaa aaagcagctc ctgaaagttc gattaaaatc
    55741 tactttcagg agctgttcaa gcttgacatt attgattttc caatccttca tacacttcat
    55801 ctgttacagg ttctaaaaac tctgaattac cagcagtgat cgccaaatgt tcaaaccaac
    55861 tgtcttttgt cgcaccatgc caatgtttaa tccctttgtg cgttacgacg acatctccag
    55921 ccattaatcg tcttgctggc tttccttctt cctgatacca gccttctcct ccggtcacaa
    55981 gtaagatttg atagccgtct gtatggacat gccagtgatt gcgacaacct ggttcaaaag
    56041 ttacattacc tacaccaaca gaaacttcgg gatcattcac taacatattc agatagcttt
    56101 gcccggtaaa tacttgagcg taagcttcgt ttttttcacc tgctggaaaa atcacgccat
    56161 tgtgtatttc ttcgtgtttt gtcatcattt acgcctcttt cttatcttcg ctattttctt
    56221 ctaaatagat ttcttttgct aaactaaaag ctgaccaagc ttttggccat tctacataaa
    56281 aagctaaatg ggtaatgatt tctgctattt cttcactagt aatgccattt tgcttgccaa
    56341 tcttcagatg agctggcaat tgttcaaaat ttcctcctga aattaatgca gatatggtga
    56401 tgatactacg ctgatgagca gataattctt ctctggacca tacttgtcca aataaaacat
    56461 catcatttaa ttctgcaaat tttggtgcaa aatctcctaa ttgatctcgt cctgcggttt
    56521 gtttttctgc catttctctt attctccctt cacgactgtt aggtgattca actatggttc
    56581 taaggcaact ttattttcta ttgtttcttc gcatttcatt ttaaatgtag tctttcagcc
    56641 tgtccaatgc ttagtttatt cagtcgatat gctttttaag catagttgct aacgcttttt
    56701 ttattttaaa aaaaacaagt tattaaataa gtctttagtt ggatgttttt ttgagatcaa
    56761 tcttttatac ttatgatgaa cttacaaaat tgtttgtttt ctgtcatatc aaaaaatggc
    56821 gcagttagtt gcttttctag taaagtatag ttaacaaaga acctctaagg agttgatgaa
    56881 taatgaatat tgctgcctct cgaaaactga taaatttcat atccattatc ggattaggcc
    56941 tatcaatcgc gctgacgatt tattttatta atttaggtgt tttcaaagat ttgaatgcgt
    57001 taagaggatt agtaggtgat tccatcattt taggaccgat tatttttgtc ttcatccaaa
    57061 tcctgcaagt agttattcca atcattcctg gagggatcag tactgcagct ggtgtcctga
    57121 tatttggccc ttatgctgga tttatctata actatattgg aatctgcatt ggttcgatta
    57181 tcatttttct gctagggcgt cgttatggaa agccattcat actttccatg attagcgata
    57241 agacatataa caaatatatt ggctggctgg ataatcaaaa ccgttttgag aagctgtttg
    57301 ctctagctat ttttcttcca gttgctcctg acgatgcgtt atgtttgatg gctggattga
    57361 caaacatgtc agtgaaacga tatacactaa tcattttgat tgctaaacca ctgtcgattt
    57421 tcttatatag tatggccttg atttatggag gtcaatatct aagtggtctt cttggataat
    57481 atttattttg taaaattagt cattgattga caatgt
//
LOCUS       DACDYM010000006        56586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDYM010000006 DACDYM010000000
VERSION     DACDYM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.006"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            81..196
                     /gene="rrf"
                     /locus_tag="IWM59_000409"
     rRNA            81..196
                     /gene="rrf"
                     /locus_tag="IWM59_000409"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            527..1372
                     /locus_tag="IWM59_000410"
     CDS             527..1372
                     /locus_tag="IWM59_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9978469.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1653..2873
                     /locus_tag="IWM59_000411"
     CDS             1653..2873
                     /locus_tag="IWM59_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9978470.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2870..5590
                     /locus_tag="IWM59_000412"
     CDS             2870..5590
                     /locus_tag="IWM59_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9978471.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5574..6518
                     /locus_tag="IWM59_000413"
     CDS             5574..6518
                     /locus_tag="IWM59_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9978472.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6587..7597)
                     /locus_tag="IWM59_000414"
     CDS             complement(6587..7597)
                     /locus_tag="IWM59_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9978473.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7777..8121)
                     /locus_tag="IWM59_000415"
     CDS             complement(7777..8121)
                     /locus_tag="IWM59_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9978474.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(8114..8548)
                     /locus_tag="IWM59_000416"
     CDS             complement(8114..8548)
                     /locus_tag="IWM59_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9978475.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8686..9423
                     /locus_tag="IWM59_000417"
     CDS             8686..9423
                     /locus_tag="IWM59_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978476.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9416..10627
                     /locus_tag="IWM59_000418"
     CDS             9416..10627
                     /locus_tag="IWM59_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9978477.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10834..11613
                     /locus_tag="IWM59_000419"
     CDS             10834..11613
                     /locus_tag="IWM59_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9978478.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11683..12345
                     /gene="trmB"
                     /locus_tag="IWM59_000420"
     CDS             11683..12345
                     /gene="trmB"
                     /locus_tag="IWM59_000420"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9978479.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12443..14197)
                     /gene="ade"
                     /locus_tag="IWM59_000421"
     CDS             complement(12443..14197)
                     /gene="ade"
                     /locus_tag="IWM59_000421"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9978480.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14329..15258
                     /gene="cpaF"
                     /locus_tag="IWM59_000422"
     CDS             14329..15258
                     /gene="cpaF"
                     /locus_tag="IWM59_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9978481.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15215..16264
                     /locus_tag="IWM59_000423"
     CDS             15215..16264
                     /locus_tag="IWM59_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9978482.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16261..16539
                     /locus_tag="IWM59_000424"
     CDS             16261..16539
                     /locus_tag="IWM59_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9978483.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16514..16984
                     /locus_tag="IWM59_000425"
     CDS             16514..16984
                     /locus_tag="IWM59_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9978484.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            17001..17303
                     /locus_tag="IWM59_000426"
     CDS             17001..17303
                     /locus_tag="IWM59_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978485.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17320..17691
                     /locus_tag="IWM59_000427"
     CDS             17320..17691
                     /locus_tag="IWM59_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9978486.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17663..18022
                     /locus_tag="IWM59_000428"
     CDS             17663..18022
                     /locus_tag="IWM59_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978487.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            18194..19201
                     /locus_tag="IWM59_000429"
     CDS             18194..19201
                     /locus_tag="IWM59_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9978488.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19226..20410
                     /locus_tag="IWM59_000430"
     CDS             19226..20410
                     /locus_tag="IWM59_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9978489.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20847..22430)
                     /locus_tag="IWM59_000431"
     CDS             complement(20847..22430)
                     /locus_tag="IWM59_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9978490.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(23008..23151)
                     /locus_tag="IWM59_000432"
     CDS             complement(23008..23151)
                     /locus_tag="IWM59_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9978491.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23374..23853)
                     /locus_tag="IWM59_000433"
     CDS             complement(23374..23853)
                     /locus_tag="IWM59_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9978492.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23926..24210)
                     /locus_tag="IWM59_000434"
     CDS             complement(23926..24210)
                     /locus_tag="IWM59_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978493.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24288..24482)
                     /gene="ssrS"
                     /locus_tag="IWM59_000435"
     ncRNA           complement(24288..24482)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWM59_000435"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24554..25840)
                     /locus_tag="IWM59_000436"
     CDS             complement(24554..25840)
                     /locus_tag="IWM59_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9978494.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25978..26649
                     /locus_tag="IWM59_000437"
     CDS             25978..26649
                     /locus_tag="IWM59_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9978495.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26615..27103
                     /locus_tag="IWM59_000438"
     CDS             26615..27103
                     /locus_tag="IWM59_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9978496.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            27116..28063
                     /gene="prmA"
                     /locus_tag="IWM59_000439"
     CDS             27116..28063
                     /gene="prmA"
                     /locus_tag="IWM59_000439"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9978497.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            28064..28828
                     /locus_tag="IWM59_000440"
     CDS             28064..28828
                     /locus_tag="IWM59_000440"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9978498.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            29061..31274
                     /locus_tag="IWM59_000441"
     CDS             29061..31274
                     /locus_tag="IWM59_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9978499.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31285..31731
                     /locus_tag="IWM59_000442"
     CDS             31285..31731
                     /locus_tag="IWM59_000442"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9978500.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31902..33161
                     /locus_tag="IWM59_000443"
     CDS             31902..33161
                     /locus_tag="IWM59_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9978501.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33250..34197)
                     /locus_tag="IWM59_000444"
     CDS             complement(33250..34197)
                     /locus_tag="IWM59_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9978502.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34591..35883
                     /locus_tag="IWM59_000445"
     CDS             34591..35883
                     /locus_tag="IWM59_000445"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9978503.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35898..37667
                     /gene="aspS"
                     /locus_tag="IWM59_000446"
     CDS             35898..37667
                     /gene="aspS"
                     /locus_tag="IWM59_000446"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9978504.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37776..38651
                     /locus_tag="IWM59_000447"
     CDS             37776..38651
                     /locus_tag="IWM59_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9978505.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38917..40800
                     /locus_tag="IWM59_000448"
     CDS             38917..40800
                     /locus_tag="IWM59_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9978506.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40875..41159)
                     /locus_tag="IWM59_000449"
     CDS             complement(40875..41159)
                     /locus_tag="IWM59_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9978507.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(41149..41883)
                     /locus_tag="IWM59_000450"
     CDS             complement(41149..41883)
                     /locus_tag="IWM59_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9978508.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            42012..42635
                     /locus_tag="IWM59_000451"
     CDS             42012..42635
                     /locus_tag="IWM59_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9978509.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42827..43948
                     /locus_tag="IWM59_000452"
     CDS             42827..43948
                     /locus_tag="IWM59_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9978510.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43955..47083
                     /locus_tag="IWM59_000453"
     CDS             43955..47083
                     /locus_tag="IWM59_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9978511.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            47102..50296
                     /locus_tag="IWM59_000454"
     CDS             47102..50296
                     /locus_tag="IWM59_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9978512.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50442..51149)
                     /locus_tag="IWM59_000455"
     CDS             complement(50442..51149)
                     /locus_tag="IWM59_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9978513.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51914..52312
                     /gene="spxA"
                     /locus_tag="IWM59_000456"
     CDS             51914..52312
                     /gene="spxA"
                     /locus_tag="IWM59_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9978514.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52644..53297
                     /locus_tag="IWM59_000457"
     CDS             52644..53297
                     /locus_tag="IWM59_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9978515.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53523..54521
                     /locus_tag="IWM59_000458"
     CDS             53523..54521
                     /locus_tag="IWM59_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9978516.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54697..56505
                     /gene="pepF"
                     /locus_tag="IWM59_000459"
     CDS             54697..56505
                     /gene="pepF"
                     /locus_tag="IWM59_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9978517.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18619 a         9768 c        11735 g        16464 t
ORIGIN      
        1 agaactcgga aaaaaactta tgtgacttca aatccagttt tgagtgaaca aaatttactc
       61 aataaaagat aacaccacag tgtggtggcg atagcgagaa ggatacacct gtaaccatgc
      121 cgaacacaga agttaagctt cttagcgccg attgtagtga ggggttgccc cttgtgagag
      181 taggacgtcg ccacgcaaag gcaaaaagat gaactagaaa tagttcatct ttttttgttt
      241 aaatcttttg ctaagttgaa ctaatagagt cttttatgaa tatcgaagtt agtttttcac
      301 ctaacttcgt tttttatagt tggttattat aaaatagtaa tatagctgat ttggattcaa
      361 ttcatattta tttataaatc aaaaatacag agaatagtat ataaatgtcc tttcctattc
      421 ttaaaatgat gtttgtactt acttaaatta ggtataatta gtatatgttt tgtgagaaat
      481 ttagacttac aataagcagt gaatttataa tcaacgtata ggagacatgg atgaaatgaa
      541 actaattggt atagatttag acggtactct tttgaattct gaccagagca ttagtcaaaa
      601 aaatgcaaga acaatgaaac atttaccgga agactgtttt ccattcattt gttctggaag
      661 agaagtagaa gacattaata atattctcaa aaaatctggt ttaaggatac cagcagtagg
      721 gttaaatggt tctgttggat ttgatggaga aaaaaagata tttgagtctt ccttcgataa
      781 aaatgatgta aaaaaaattg attctgtcct atcgaaattt ccaaccaaaa tatatacaaa
      841 tctcggaagt tatgaatctc agaattataa aaataaaatg aaaaaaattt ttcacgaggt
      901 agggagtgaa ttttctatca aagagctaaa ctatgagctt gagtatgaaa aatctatttg
      961 ttcaatccct tataataata ttgaggaaat tatagaaaag gatgctatca gcatttacaa
     1021 attttttatt tttattccta atcgtcaatt gaaagctaat atatttaacc atttgaaaaa
     1081 gatgctaaat gtgtctgtta ctgaatcggc acctgtaaat attgaaatcg tacctcaaaa
     1141 tgtatctaaa ggtgtggtgt ttgatcgttt ggaatcaatc tacaatttaa agaaaccttt
     1201 gagaatcgcg atcggagata gtttaaatga cttatctatg tttgaaagtg cagatattag
     1261 ttttgcaatg ggaaatagtc atcaagaaat catgaaaaaa gctgattatg tcactgtttc
     1321 aaatgataaa gacggagtat ctcaagcatt tgaaagaatt cttaccatat aaactaatta
     1381 aggtagtata gaacatagaa ttctaaatac ggaaaaatgg agcataaatt ccagtacaaa
     1441 tagtacttct tcagtgtcaa acgaagcgaa tataacatag atatttagga ttgtgacaaa
     1501 aatcatgtca caatcttttt ttcgaataaa cttatttttt gaagtcggcc atttatcgta
     1561 atttctttgc ataatacgaa caaattttcg gttattgatg aaaaaaaaca ttgaaaatgg
     1621 tatgataaaa gaaaaaaagg aggaaatttt taatggtgaa atttatccat gcggcagatt
     1681 tacatatgga tcggtcattt gaaggactga caactcttga taaaactgta caggaaaaac
     1741 tattgaagac gaatctgacc gttttatcaa atatcattga acaggcaata ataaataacg
     1801 ttgattttgt tttacttgca ggagataatt ttcatcaaaa tcgtccttca ttaaaaatac
     1861 agaaacattt ttcagaacag atgaaacggt tagaaaagaa ccatatacct gtatttataa
     1921 tattcggaaa tcatgatttc tatcaaaaag agcggtattg gttctccttt cctaaaaatg
     1981 tccatttatt tacatccgaa acggtagaga caaaaaaaat aacgataaaa agtggagaaa
     2041 ctgtctctct cagtggattt agttatcgtc aaccgtggat tcaaaaagac aaagtgatgg
     2101 aatttccttc tagagaactc acagattacc atattgggtt gtaccacggg gaacctggcg
     2161 tctctcaaaa aggaaactat gctcctttcc aaccctcaaa gatgcaagaa aagggatacg
     2221 attattgggc tttagggcat atccatgtgc caacggtctt aaatgaaaag caaacaattg
     2281 tttatccagg tgcgcctcaa ggtcatacta aaaaggaaca agcttctaca tctattctac
     2341 tagttgaatt aagtaaagga agttgccaaa tacatccgat taaagtagca gaggtctatt
     2401 ggaagacaaa ggaaatatca ttgaggaaag caagaacgac aaaagaggtc attgttcaca
     2461 ttcgtcagca gctttcaaaa gtagaggatg gatttagttt aattgagata aaattaaaag
     2521 attatgacca tctcaataca gatgttttgg aacggataca atcaggagag ctattagact
     2581 atttgttaga ggaattctca gataggataa atgatttctt tatttggcgt atttctctca
     2641 tagagaatac tttttatacg aagaccccac ttgctgcttc tgctaaattg atggaacagt
     2701 tatttcaata ctatcagaca cctcaagatt tccaacaaat tttaaatgaa gtatatagtc
     2761 atcaagaagc ggctagaata ttgagcgaac tgccggaata tcaagaagaa acacttgaaa
     2821 aagccaagca gctgcttaat caagattttt tatttgagga ggatcaggaa tgaagatttt
     2881 aaggatcgag attgctgctt ttggaaaatg gcgtcaaaaa agttttgact tttattcagg
     2941 caatcagctg atctatgggg gaaacgaagc aggaaaatca acgatttatc aatttattca
     3001 agcaatatta tttggtttcc cttcaaaagg ccgtaaaaaa aaggattata caccaaaaga
     3061 cggctcagcc tatggcggca aaatatggct taaacatcct gtatatggtg aatttgctgt
     3121 cgagcgctac aagcagcaaa atcgcggaaa atcgaaggtg tggctggggg atcaagtggg
     3181 aagtgatgag ttactagaaa aactgatcgc acctttgacg aaagagttat tccagcaggt
     3241 atttactttc cagcaagagc agttgatgga actagatcat ttgagagaaa aagagctaca
     3301 tgatgcgctg atttctttag gcatcacggg cagttctcaa gtattccaaa agcggcaaga
     3361 atattatcag aaagcacaac aattattcaa gaaaaaaggc caaaaattac taatcaatca
     3421 aaaattaaat gaatggaaag agctgcaaga aaaaattcat caaaaacaag aacaggaagc
     3481 ttattttggc aagttgatcc aacaagaaca ggaatacgag tcgcaacagc agcagttatt
     3541 ggcacaatta aaagaggcga acaaacaatt ttcattagcc agacagcaag aacttaattt
     3601 ttctttatat gaagaatggc aatctctcaa aaaaaacagc actacaggtc ttcctgatga
     3661 gcaacttctt ttagatctgg agtctttctc caaacggtat cagcaaacaa atgagcgttt
     3721 ggaagaatta gaagcaacac tggaaaagca tagcgggatg gatcagcagt cagccagata
     3781 ttatttttat ttagagcagg agcaggaact aaaagatctc cagcaacttc aatttacagc
     3841 ggaacaatta aacgaagaat ggcaaagatt acaaattgag actagagaaa caaattcaga
     3901 attgaagatt ttggaacaaa ggtggcattg gcagaaaaat ccgttaccgg aagaattttt
     3961 atatgaagaa gaatggaagc atcttctggc tgaagaaaga aggattgctg atctagaaag
     4021 ccagatttct gtgcgtctcc aaatagctaa agaacagcaa gagatgattg aagcagaagt
     4081 gaatgacttg gaagagacct atccagcatt attacaaaat cagtcagaga tagcaccagt
     4141 ctcttcatct aaaccgtata tgctgatggc tggcggagca ataatcattg caggtatatt
     4201 tgtaccaatg cccttgaaag tgatcctttt actaattggt gcaggcatga ttggctattt
     4261 cttctacaaa aggcaaccaa aaattaaaac taaaaatgat ttctctgaga taaaaggaat
     4321 gtggcaagaa aaactaggac aacttgatct agccttagct caattagctg aagcagaaga
     4381 aaaacaaaag agaactggag aagaaaaaca gattcttcag caagaagtat atcgttttgc
     4441 ggatcatcgg cacttaggaa agatggatac gctcaaactc atgaaagaat atggacagga
     4501 agtcatagat tatcagaaat tatatggcgt atttgagcgg aaacataaaa gagaacagga
     4561 aatacaagaa aaactacgac aaatcgacca aaaatttgag tttgttaaag aatggttacc
     4621 gatacaagat aagacaattt cagaaaaaat ggtattgctt tcccgtttta tacaagagat
     4681 ggaacagatg aaatttgcca gaagctatca gcaaaatact ttattgaagc aagaaatcaa
     4741 tcaattgaaa aagaaacaaa aagtatggat acaagaatac agcgaacaat tgactcaagc
     4801 aggcttatct tatccttcgg aagtacctgt ctttttacaa aaagccaaac aagccgaaca
     4861 gcagatgaaa agatacaatg aattatcgga aatgcttact ccactatttc ctgaagagct
     4921 gacaaaagag aaattatctg taagactgcg ccaactgaaa gaaaagcaat cccaactcca
     4981 gcaagaagaa aaagaagcaa gtgaaaatcg gcaacgactc caacttcaag tagaagagtt
     5041 gcaaagagac ggcacattgg acgaacttta tcaacaagct agccggcaaa aagcaaaatt
     5101 acaggaatta ctggaggaat ggggagcact tgaaatcgcc gggacattct taggtgatct
     5161 ttcaacagag atgagtgaaa gacaattacc ccagttgtta agagcagcag gtgattattt
     5221 aggtatttta acaaaaggaa attatcagaa gatattactg aaaaatgata cactgacttt
     5281 aacagacgga tatacagaat ttcccatcta tgagttatct acaggtacga aagatcagct
     5341 gattatggcg atgagatttg cttacctctc attagaagct aaccgttcga tatgcccgat
     5401 catcattgat gatggctggc ttcattatga tcatcagcga aagtatcaat tagctgaact
     5461 tttagctaaa tttggagaaa agtatcaaat aatttgtttt tcttctgata aagaaatggt
     5521 aagctattac catgagatga accaaatcgt aaaacaatta gaaggagcaa ataatgaaaa
     5581 aaattcgtga tttagtaatc gatgagcagt ttgaacaatt tgtcctgatc aaaagtgcag
     5641 atgtaagaat cgctaaaaac ggaaaaaaat ttattgcttt tactttccaa gatacttcag
     5701 gtactatcga tggtaaatac tgggatgctt cggaggagga aatcaataaa tttacagccg
     5761 gcacagtcgt ttttctaaac ggtaaaagag aggtttatca aggaaaccct caagtaaaga
     5821 ttcttcatat gcgcttaaca agggtagatg agccaggaga gcctagttta tacatggaaa
     5881 gagccccttt gaagaaagaa gaaatggaag aagaaatcaa tcaattgttg tttgagatca
     5941 ccaatgcgca ttggaatcgt attgttcgct atctacttaa taaatatcaa aaacaatttt
     6001 ttgagtttcc ggcagcaaaa agaaaccatc atgcatttgc cagtggatta gcttatcata
     6061 ctttgacaat gcttcactta gggaaatcaa tcgccgatga atatcctgag ctgaatcgag
     6121 cattgttgta tgctggtatc attttgcatg atttaggaaa ggtgattgaa ctttccggtc
     6181 ctatgtcgac agaatatacg cttgcaggaa atttgatcgg tcatctggta ttgattgacg
     6241 aagaaataac taaagcctgt cttgcgttga aaatcagcga agctgacgaa gatgttctcg
     6301 tattacgtca tatggtttta gcacaccatg gcttattaga atatggctca cctgtacgtc
     6361 caagaataat ggaagcagaa atattacatc aaatcgacaa tatcgatgct tcgatgcaga
     6421 tgatgttgac ttcgattcgt caaacagaac caggaaaata caccgatcgg attttcggta
     6481 tggacaaccg aagtttctat gtaccaaaag atttatagca aaaatttatt tttttcattc
     6541 aaaacaaaaa aagcgagaca taagtcttcg ctttttttgt tttcttttat tttgatgaat
     6601 cagttgtttc ttcagttgaa gattcttttg ttgaatcagt tgattttgtg tcagaactct
     6661 ttgttgaagc tgtagtttca cttgagtctt tcgtacttga tgaagttgtt gtgaatgcac
     6721 tcaacacatt ttcaaaggca tcatctttga tttttacatt tgcatctttt aattcttcac
     6781 cgattacttt agttgtgaat gcattgtcac ttagtttagt ttcagtagcg atatctttta
     6841 gttgatcttt gtatttatcc atgtcattgc ctttattttg gtttttcacc attttgacaa
     6901 cgtagtattc tgtcgcataa gaagtaggat ttgttgtagt aataacatcg gagatctcac
     6961 cgtctttcaa tttgaaagct gcttctttta cttctgcagg aatagttgtt gtcgttgagt
     7021 cgaatttaac tttaccgcca tcttcttttg tctctgtatc agttgatttg tctttggcta
     7081 atttagagaa atcatctcca tcatccgctg attttttcac atcttttgct tcatcttcac
     7141 ttgataattt gatgatttgt gcttctactt ctggatggaa agatttccaa gcagttttca
     7201 aatcgtcatc tgtaatatct acgtgagctt tcaagccagc ttcaaaagct aaattgttac
     7261 gaatatattc tttataagta tctttcgtat atccagcagc ttctaattgt gattcaaaag
     7321 tatcacctaa tgatttcgct tgtttgtcgt attctgcatc cacttgctta tcggttactt
     7381 tatcgccata tttgttattg aatactttat aaatgatcat ccgttgtact aaggattggt
     7441 tcgatgattc aagttttgct tcatcataga aatcagatac agtgatcgtt ccgcctttca
     7501 tcgttgcgat atctttatta gtatcgcctg aacaagctgc tagagttaaa acagccaacg
     7561 cactagtagc tgccaaaata attgattttt ttttcattaa aatttgcact cctgtaatta
     7621 gttattattt ttatatatta actataccat atcccatttc ctttaaaaac gttgtcgtgg
     7681 tagtttcaaa caaaattcaa aatttcatca cattcctagg aaatacgcaa acttttctca
     7741 cttatatagt taaatctgtt gagaaaataa tcattattat atttgttcag gatactctgg
     7801 cagatcagct tttagttttt ctacttgttc attgatttgt gtgatacgtg gttcggcttg
     7861 gaatttaaaa tcttcgatat cttttttgat tgcttcttgg aaagcaggaa tcacagattc
     7921 agctgtttct tttgtttcga tcaatgcgct tttaaaatga ttcaggctgt tatttaaatc
     7981 atttgtcaga gttgttgctt cttccaattc ttcgatcagt ttctttcttg tttcattccc
     8041 gctcctgggc gccaataaca gacctccgac agtgccagca gcagctccaa ataacagtcc
     8101 tttgaaaaag tttttaatca tcgcaggaca cctgctttac gattgtttct tgaatagctt
     8161 ttagttcttc cgatccataa ctatcttgat gattgacaaa gtgaatagaa aaatcatcct
     8221 ctttgctgta gcgagggact aaatggatat gtgaatgaaa aacagattga taggctagtt
     8281 cacggttgtt attcaagata ttaagacctt tcatttctgg aaaagctttt tccaatgctt
     8341 gagcaatctt aggcacacgc gaaaaaacct ctcctgcaag ttcgggatca tattcaaaaa
     8401 tatctgctac atgacgttta ggcacaacta atgtgtgtcc tttcgttgct tgagagatgt
     8461 ctaaaaaagc atagaccttg tcgtcttcat atattttata gcttgggatt tcttgattga
     8521 tgattttgca aaaaatacag ttttccatat aaaacatcct ttctataaag aataaatact
     8581 tcagcttaac tttaccataa gttaaagcta ttgtaaggaa aaaaacaaaa taccgtcatt
     8641 ttcttcgtgg tataataaat aaaaataaat ggcggaggaa aaagaatgag tttaattatc
     8701 gatcatgtca caggggggta cggacatctc cctgtgttaa aagatatcag tttcgatgta
     8761 cagcctggag agatagtagg gttgattgga ctgaacggag cagggaaaag tacgaccatc
     8821 aagcacatta tcggtctgct ggaagcttca aaaggaacga tagaaatcaa tcatctatcc
     8881 ttaaagaaaa atacggaaga atatcgcaaa caaattgggt atattccaga gactccttct
     8941 ttatatgaag aattgacatt gagagaacat atcgaagtca ctgcgatggc ctacgatata
     9001 cctatggata tagcaatgaa aagagcagaa aagttgttgg agacttttcg gttatctaat
     9061 cgtcttgact ggttcccagc taatttttca aagggaatga aacagaaagt gatgatctta
     9121 tgcgcctttt tgatcgagcc ttctttatat atcatcgacg aaccattttt aggcttggat
     9181 ccactagcta tcaatgcatt gcttgaattg atggtagaaa tgaaaaaaga aggagccggt
     9241 attttgatgt ctacgcatat tttagctact gcagaaaaat attgtgatcg ttttgtggtc
     9301 ttacataacg gagagatacg agcagacggt tctcttgaga tgttgagaaa agaatttcag
     9361 ctgccggatt cttctttgga cgaaatctat attgctttaa caaaggagca ggcacatgag
     9421 tagttttttt caaaaacgtt tagcaagaca tcaaaagaaa atgatgaaat atatgcgtta
     9481 tgtattgaac gatcattttg ttttagtttg tcttttcttg attggcggtg tgggattata
     9541 ttattctaat tggttgaaaa ccctgcagcc accgtttgca attggagggc tcataatctc
     9601 tgtattttgg atgctctgtt tattcgtggg taagatggct acttttgcag aggcagcaga
     9661 tattgttttt ttacttccta aagaaaaaga aatgcaaacc tatttggaac gagcttttcg
     9721 ctattcttgc atttttccgt tgatcttttt gatattggca tgcggattgg ccatgccatt
     9781 agtggtggtt agtacggagc aatccttttc gatgtttttc gtctatctga tcatattatg
     9841 gagtttgaaa ttcagtcatc tgcaaatcaa gagagcagaa ttgtttcgac tagatcaaca
     9901 agttattcgt tattggaaat taggctggtt tttttcttct ctgatctttt tgtttctatc
     9961 attttattgg ctaatatggg caggaatagc aggtgcatta gtacaggcaa gtctatatta
    10021 ctggtttttg tggaagcaac aagaaaaagg attggattgg gagaaaatgg tgcatgtaga
    10081 agaacatagg ttgcatcaga tctatcaatt tatcaattta ttcacagatg ttcctgaaat
    10141 tacggcaaag atcaaaagaa gacgctattt ggatcctata ttgactcata ttaaaagaga
    10201 atcaaaaaac acttatcttt acttgtttgc aagaagattg gcaagaggtt ctgattttag
    10261 tggtctttat ttccgattag taattatagg tgggttattg attgccggag tagtcgactt
    10321 ttattttagt ctgattatcg gtttcttatt tttatatttg atcggtttcc aactgttacc
    10381 tttagcgaac cagttccgct atatggttct cgttcagctt tatccaatcc ataagaatca
    10441 gaagaagcag gcgattcagt atctactcac atgggtatta attatcgcgt cggttatttt
    10501 tggtttgatt gctgcagtcg tattagatgg gcagcagcga tgggttccgc tagcggctta
    10561 tttgattgtt actatgctct ttgttaaact atatgtacca actcgtttga agaagatgtc
    10621 agactgaaaa caaagctgtg tagggtaaaa atgagaatga aaaatagagc gttgacagta
    10681 agaatgctgt tcgttctatt ttttttgaga aaagctggtt ttttgaatgg caattaaatg
    10741 aaaaagaacg tatatgtgct tgacgtgtag tgttcaggcg actagaataa taagtactgc
    10801 aaaacaaaga gtaagaggac gggagaatgc atgatggagt ttcagttaga ccaagagtgg
    10861 cggttacgtc ccatcaaagg cgacacagga aaaacataca taggcatgcg tgatgaggac
    10921 aaagtcttta ttaaacgaaa cacgactccg atgttggctg ctctttcaag ggaaggaatt
    10981 acaccaaaac ttgtttggac aaaacgaacg gggaatggcg atactttgac agcacaagag
    11041 tggctggaag gtcgcttgct gactcctaat gagattggtc aaagaaacga cgtgattgat
    11101 gttttgtacc atttacatca ttcaaattct cttaaaacca tgctgacccg tatcggtggt
    11161 cgtgtcatga cgccggagaa gatgctgcaa aaatatgaaa aagaattacc ggaagaactc
    11221 cagaaaaatc aatttctgca aattgtccat cgttttcttc gtgagcagat cccgtcaata
    11281 gataaggaaa acttatcagt agtacacgga gatgtaaacc atcgtaattg gttagtatgt
    11341 aaaaattatt tatatctagt cgattgggat tcaatcatgt ttgcagaccc tgcggtggat
    11401 attgcaacga ttttaggaaa ttatgtgcca ctgtctagct ggaatcagtg gctgatcagt
    11461 tatggcatac gcccgactga cgaaatgctt gaaaaatttc attggtatgc ggccatgaat
    11521 ttgctccaag aaatcaccag atattgcaaa cgtgaagatc atcgacggat gaacgaagaa
    11581 attttacaat taaaacgaat tttcagtggc tgaggtttgg cccaaactct gcggagattt
    11641 ggccagacct ttttaattag aggaaaggaa tggagataaa taatgcgtgt ccgaaatcgt
    11701 ccgggagcag cggaaatgtt agcggctcac ccgaattttg taattagtga tccaacattg
    11761 tggaagggaa aatggaatga attgtttgaa aacgatcatc caatccacat tgagattggt
    11821 atggggaaag gccagttcat cactggaatg gcaaaagccc atcctgaaat caattatatt
    11881 ggagtagaaa tgcaggtcag tgttgtttcg attgcactag acaagttgat cgaacaaccg
    11941 cttcctaacc tgaaacttct acatgtggat ggatcagcgt tgacagagta tttcgctgac
    12001 agcgaagtag atcaaatcta tctgaatttt tccgatccat ggccaaaaaa acgtcatgaa
    12061 aaacggcgat tgacatataa gacttttctt gctgtagatg aacagattct tcgtccaaat
    12121 ggagaaatcc atttcaaaac agataatcaa ggattgtttg agtattctct ggcaagtttt
    12181 tctcaatatg gcatgatcct caaacaggta tggttagatt tacatcaaag tcaatttgaa
    12241 ggcaatatta tgacggagta tgaagaaaaa ttttcttcaa aaggtcaacg tatttatcga
    12301 gtagaagctc aatttcaaga taagaccaaa aagaaaagtc tgtgaaacta actttctgct
    12361 ttcataaaaa cattaatatc cataaataaa tcccagaaaa tgaacattac agatgtaatg
    12421 tattttctgg gatttatttg atttaaaatg tatttatttc atcggttttt ccacaaggtg
    12481 gaatcaattg gaagcgagaa aatgaaaata atcctttgtc ggtgatcttc aattcgggta
    12541 tgacagataa tgttaagaaa gaaagtgtca aaaaaggatc aaaggtttta gctgctccta
    12601 gttgatgtgc tttagttatt aattcattca actgttcata gacgatcgaa aaaggttcct
    12661 gtgtcatgat tccgcctata ggaagagaaa cacaagctat ttcctgctgt ttgttgacaa
    12721 cggtcatccc gccacctttt tgaatcaaaa gattagcagc atacagcata tcctcgtcgt
    12781 ttgtcccaac gacaactaga ttatgcgagt catgggcaat cgttgtcgct atcgctcctt
    12841 gtttcagctg aaatcctttc actatgccta atccaatgtt tcccgtttga tggtgacgct
    12901 cgatcaccgc aatctttaat tggtcttttt cgattgaagg aatgaatcgt tcgccttgtc
    12961 ggtccacttt ttctaccaaa tctttagtca ctagactatt cgggataatt tcgatcacat
    13021 gggcttgtga agaagaaaga agcagctcta acgaatgtgg atttaatggt tgagcgttta
    13081 gttttggcag acaacacgta tatgaatttg aatgatcagc aaataaatgg tcttggagtt
    13141 gcccagattg aacaactaat gttcccttat agaaaacttc atgaattggc agcttggtaa
    13201 gatcgtctgt taagaagaaa tcagcttgat acccaggggc aatcgcacct agtgttttca
    13261 aatcaaaaca ttctgctgca ttcaatgttg ccatctgaat ggcttgtaat ggatcgattc
    13321 caagtgcaat agcttgacgg acgatatgat cgattgatcc ttcttttact aaatcattga
    13381 tcagtttatc atcggtgaca aagaagcatc ttcttgaatt ttcaggtgtg atcgccggta
    13441 gtagcgatgc cagatctttt gcaacggtcc cttctcgaac cattaaatac ataccaagat
    13501 ccaaacgttc tttagcttct tttgcagttg tagcttcatg atccgtccga atacctgctg
    13561 caggataaac atttaactct tccatgccga taccagctgc atgcccgtca atttttttct
    13621 tatgctggtg catcaattgg atcttttcta ggatgtctgt ttcattattt gctactgctt
    13681 gatagttcat gacttcagct aagcctaaaa cttcgggttt ttgtaaaaaa ggggctaatt
    13741 tttttgcatt taaatttgca ccattggtct caaaaggggt tacaggtaca caagaaggta
    13801 gcataaaaaa gatattcatg agtgtttgtc ttgcatcttc tatcataaac tcaagacctg
    13861 tactcccagc aatgtttcct atttcatggg gatctgtcac tacagtagta actccatgaa
    13921 gcaaggatac ttttgaaaat tcgcttggtg ataaaagtga gctttcgata tgcacatggc
    13981 catcgatgaa tcctggcact agatattgcc cattagcatt atgtacgctt tttccttcgt
    14041 attcaccgat tcctacgatc attccttcgc agattgctac atctttttcc atgatttctt
    14101 tcgtaaagac gttgacgatc ttagcatgct tgatcactag gtcagcaggc ttttttttac
    14161 ttgctacttc gatatatttc cttaatgttg tttccaatgg agtgcctccc aagataaatt
    14221 tttataagta taacataaat tcggtttttg ccaatatttt tttgtaaatt cattttattt
    14281 gaaacaaaaa gatcaaaaag acgtatgttt tttgtggagg ggagaataat ggaaataaaa
    14341 cgattatcgg atcaaatgat aagtgaaagt tttgccaaac atgcccaaga cgtatatata
    14401 ttgccagctt cagaagtatt ttacatctat tttcgctatg gaaaaaaacg aacattctgg
    14461 aagaaagtct cttcggcaga tggacagcaa ctgatctcaa gatttaagta tttgggacag
    14521 atggatatcg gagaaaaaag aaaagctcag ttaggtgcat ttacttattc tttagaaaaa
    14581 caggaagttc gtctgcggtt gtcttctgta ggagattacc ttcaacgaga gagtttagtg
    14641 attcggctat tatacggtac ttttgaaaca ccccatcgtt gctttagttc tcgagatttt
    14701 gcaactattt tacagaaaac cgtcagacgt ggattatacc ttttcagcgg accagtcgga
    14761 tcaggcaaga cttctctaat gtatcggttg gctttggaag aaaaattgca agtgattact
    14821 atcgaagatc ctgtagagat agaagaaccg ttttttctgc agctacagat caatccgaaa
    14881 atagcacaga cttatgatac acttttgaga ctcgcattac ggcatcgtcc agacttgcta
    14941 atcattggcg agataagaga tgaatcaact gcacatgctg cggtaagagc agcactaaca
    15001 gggcaccggg tttttgcaac cgtacatgca cgcaatcttg aaggtacatt ggtcagaatc
    15061 catgaactga caaagaaaaa agaagagcta tctgaatgtt tagctggtgt catttatcaa
    15121 gaacttttgg aagatgccaa tcaccatcct tctgttttat ggagttatca atttatggag
    15181 gaagaatcag taaaaaaatc atgggaggaa agttatgaag aagctcagga aaatatttgg
    15241 cagtcagaac tattctaaag gattaacaag aagccagcaa aaccagtttg cacaaaccat
    15301 gggagaactg cttttatctg gttttagtct gcaagaatcc ctttatttat tactcacgat
    15361 ccgttcgttt ccacctgtta ttttggcaaa tatccaacat cagctaaagg aagggcaagc
    15421 gtttagtgag gtactttcgc aaataggata tggagcagag caactggtac agatcgagtt
    15481 agctgaacgt catgggaatc tgccgacaac tttgttggaa attgccaaac agtttcggct
    15541 tgttgaagga tttcgcaatg atttgaaaaa gctgcttgca tatccgatgc tattgctcac
    15601 ctttttgcta ggtatcttgt ttagtatgcg gttgttcgtt ttgcctcagc ttattggttc
    15661 aggaatggtc atgcaagagc attggggtat tcagctgata caagtctttc attggtatgt
    15721 tctactattc atcctagcgg ttactctatt ggctttacta gtaaggtatc gtctgatgaa
    15781 gttgtctcct gttgatcaga gtgaatggat aagcagacaa ttattgattg gaccgatata
    15841 ttccttgtat caatcggctt atttgtcact agagattgga aagctttttc atgaaggttt
    15901 cgaattaagg caaatcatag tctgcttaaa ggaaacgaaa caagaaagtc tgattcagca
    15961 tcttgcctct aagttgattc aaggattgga ggcaggtatg tcattggctg agcagttcca
    16021 gaattatcgt tttctatcag aagaattcag taaaattgtt ttacagggag aggcaaaggg
    16081 aagtttaggt aaagaactga ttttttacag cgaatggaca agaaaacagc ttttcaaacg
    16141 tcttcatcac tggatgcatt ttattcagcc aatcattttt ttgggcgtag cggctttgat
    16201 tttattagtt tatgccgctg ttttattacc tgtttacgga agcatagagg aggttttgcc
    16261 atgaaaaaga gaaaaatatc ttattatagc ggttttacat tgatcgaaat gctaatcgtc
    16321 ttactgatca tcagtgtatt agtgttgttg ttcgtcccta atttgtcgcg ttataggaat
    16381 catgtagacc aagaaagtcg tgaagcgatc atccagttgg tagacacaca aaaagagctc
    16441 tatgcgcttc aaaataatgg aagagttcct actgtggagg aattgttgaa tgaaggctat
    16501 atcaaacgag aacatgcaga aatctatcag cggccttgaa ggtaaggttt tagcatcagg
    16561 atatacgttg tttgaatcac tcttactgat tttattactt acaacgatcc tttcttttcc
    16621 agttctcgca ttttctgcat ggaagaatga attggcagtc tatcaatttt tcggccaatt
    16681 tgaaaaacga atctattcta cgcaaaaaat cgctattgtg aatcagatcc aaacaggctt
    16741 ttattggaat agtgaagaga atcaaatcat ttttcatgtt ccgaatccag ataaaataaa
    16801 ttgggaagta ttagatattc ctgatgagat taccttgaaa cgccatgctt ctattacttt
    16861 tgctgctaga accgggaatg aaagttcact aaaagcttat caattttatt gggaagaaaa
    16921 agagcggacg attacgtatc aattccagat agggagtggt cgctatacaa aaagaattga
    16981 ataaaacagg atttgttttg ttggagtccc ttacagcgct tatgatcgct tctgtctgtg
    17041 tctacttagt gagctttggc cagctgtttt tgcttcaggc agaaaagaaa agtggggagg
    17101 aactgctatt tattcgaatg atttatgaag atatcaagac gcagcggatg tatcacaaaa
    17161 gaccagagcg aagggaagaa atgatcccat acgatatcca aattgtcgat aataatggag
    17221 taatcgaaaa agtgtttgtc aatatggaga aagaagcgat cagtattgaa aaaacaagta
    17281 attccagcat ttacgctgat tgaatgtcta attggactga tgatattgag cagtctattg
    17341 ctcacattca atcttttgat tcagcaaacc atccaagtaa gtgaattttt gcagcgtaaa
    17401 gatcaaaaag agtggctaat atttttagca caattagaag aagaactcaa gagcagtcac
    17461 aatgtctctg tgaaaaatca gcaactgcac tacacgatcg aggataaaca gtatatcatc
    17521 gaaagatatc aggcaatgat acgtaaaaga agaacttccg gcggacatca acctatgtta
    17581 acaagtatca ctgagcttca attattggaa aaagaacaca caatttcttt ttatgtacac
    17641 tttgaaaatg gagaagatgg atatgccata tggacaaaaa acgatcagta gaaaatgtta
    17701 taagggctct attttatttt ctgtcttgct tttatttctt ttatgtacgt ttttattgat
    17761 ggcagcatta gaaagctacc gtttgtcaat ggatcttaat atacgaatca aaaattatta
    17821 tatagcaaaa ataatgaagc agatggcttt atcggaacag tctacgcttg ccgaaagttc
    17881 ggaaggagtt ttttactaca caactggatc tgtaacatat cagtgggtac aacagtccct
    17941 attcttagag gtggaagtct caccgtttat ctttcggttc acagaagaag taaaaaatga
    18001 tacagatact agtacagaat gatggaatgt tcgtgaaaac gccctctttt ctgttataac
    18061 actgttttta ataaaaaaat gtttgccgtt tcacaaataa aaaacagtga aaacgattga
    18121 aattttgtca attgttcgat agaattaaac aggtgaaaaa attgttataa tatatctaga
    18181 aatgaggtgc accatgttac cagaaaaaat ggaacaaggg tttaatcaaa acctggaagc
    18241 tattcagctt ttacagaatg cattgggaac ttccttttta gaagcctatg tcgaaaatgc
    18301 agaaaattta atagatgatt atcaagtacg tgtggtggat ggagtgccga caaaagagac
    18361 aacgcaacgg atcacagctc tttatgaaga gttgaagaaa ctttctttcg agccagaaga
    18421 atggcgcaga ctgtctcaat tacttttatt gaagggcagc cagacagaac atttgcaggc
    18481 gaaccatcaa ctcactcctg atagtatcgg ttttttattt gtttttctta ttgaacaact
    18541 atacacaaat aaaaaagcac cggttaagat tctggatatt gcggcaggaa tgggcaactt
    18601 gttattaaca gttcttttaa acctatcgaa tgcggggtac caaacagaag gaattggtgt
    18661 ggatattgat gatacattgt tggctgtcgc agcttctaca agtgatttga cacaagcaaa
    18721 tgttcaatac tttcatcaag acgggttgca ggaattatta atagatcctg tcgattttgc
    18781 aatcagcgat ttgccaatcg gttattatcc gaatgacgaa aaagcaaaag agtttttaac
    18841 aagtaccgaa gaaggccata gttatgcaca tcatttattg ctagagcaat caatgaaata
    18901 tgtgaagccg gatggttttg gtttgttttt gatgccgagt ggatttttag aaacggatca
    18961 aagtgaagaa atcaaaaaat ggtttaaaga agaaggttat cttcaaggaa tgattcaatt
    19021 gccagatgaa ttgttccgta ataaacaatc gcaaaaaagt attttgatct tacagaaaaa
    19081 aggaccacaa gcacaacaag taaaagaagt cttgcttgtg aaacttgctt cattaaaaga
    19141 accagagaag gtgacggaat ttttcaacga gtttaaaaac tggaaatcat ctgctctgta
    19201 atcattcaac gagaaggaga gtcatatgtc taaaacaatt gcaatcaatg ctgggagttc
    19261 aagtttgaaa tggcaattat atcaaatgcc agaagaaacc gtagttgcga aagggatcgt
    19321 cgaacgtatc ggtttacaag attctatttt taccatcaag tacggcgata atcaaaaatt
    19381 cgaacaaatc atggatatcg aaaaccacga agtagcagta aaaatgctgt tagatcagtt
    19441 gattgaactg caaattttaa gctcttatga tgaaatcacc ggagtagggc accgtgttgt
    19501 tcacggagga gaaacttatg gggattcagt agtgatcgat gagaaagtaa tggaccgcat
    19561 tgctgaatta gcagaatttg ctcctttgca taatcctgca aacttaatgg ggatcaaagc
    19621 gtttaaaaag attttacctg acattttgag tgtggctgta ttcgatacat ctttccactc
    19681 aacaatgcca aagcaaaatt atttatatag cttaccggtg tcttattatg aagatttcaa
    19741 agtaagaaaa tatggcttcc atggcacaag tcatcgttat gtgtcggaac gtgcggcaaa
    19801 attattgaac aaaccgatcg aagaactaaa aatcatcact tgccacttag gaaatggtgt
    19861 atcgatcaca gcagtcgatg gaggtaaatc catggatact tctatgggat tcacaccatt
    19921 agctggggtg acaatgggta cacgttcagg tgatatcgat ccagctgttc tcccatattt
    19981 aatggaaaaa ttagagattg atattgatga aatgatcaat gttttgaaca agaaatctgg
    20041 tctgctaggc ctaacaggat tgtctagtga tatgcgtgat cttgaaaaga actatgaaaa
    20101 agaacatatt cgtttagctt atgacatttt cgtggaccgc atccgaaaat acatcggcgg
    20161 ttatgtgaca acgatgaatg gtgttgatgc aattgtcttt actgctggta tcggcgagaa
    20221 tgacggacat gtacgtagtg aaatcatcaa aggtatgtct tggtttggtt gtgaaatcga
    20281 tccagcgttg aatgaattgc gtggtgaaga agtagatatc tctacaaaag aatcaaaagt
    20341 aagagtatta gtgatcccaa cagatgagga actaatgatt gcccgtgatg tggagcgtct
    20401 gcgcggctaa gccaaagcat tcgttaggat aaaagatgaa acgcatgcaa gagggaattc
    20461 ttgcatgtgt tttttttgcg gagaagaccg caaaatggta atttaacttc aatttccctt
    20521 cttttttaca ggatcttctt agggaatttt gcttggaaaa gtggtcaaaa gggaaaagac
    20581 tggcatttta tttattgatt ttcaggaaaa tcccaatatt ttattttcat tcatctttat
    20641 aagggacatt acatatgtaa aaagaaaaaa catttttaga tcagcctcgt gggctgtcga
    20701 ctttattctt cgctttatgt ttagctagaa aaccgatccg aaacattcat tgatcgaata
    20761 ataaaaactt gattttccac ctgtactttg cctgaatcgt gaaagcacag gtatgaaaat
    20821 caagtttttt gttgcgaaaa aacgtcctag tcaatcagtt ggccgagcgg ctggcggctg
    20881 atctgctcct gatcaataac gagtgcttct tggaagtgtt gctgcaaaaa gtagcggtca
    20941 tggctgactg ctaggagcgt tccttgatag tcttgcagca tttcttcaat ttcttctcga
    21001 gcatagctgt cgaggtgatt ggttggttca tccaaaatca ggacgttgat tttttctaaa
    21061 aagagtttca gcaacgccaa acggattttt tcaccgccgg ataaatcttt gatccgttta
    21121 cccacatcgc tttgataaaa gccacttttg gctaattgcc gtcgagcgac ttcttcgttt
    21181 ccagtcaatg tgatggcgta agccagcaaa cggctatcgg gatcggcaaa ctgcagctgc
    21241 tgcggcaaat aaccgatttg caagctacta cctaatttga tggtgccacc gtaaggcttt
    21301 tcctcaccga gtaataattt gagtaaggtt gattttcctg caccattgtc gccgatgatg
    21361 gctaaatgat ctccacgata gactgcaaaa ctgctgtctt tgaatagtac ttgatcagcg
    21421 tactgctggt gcaagttttg aatgatgaaa acttctttgc cagattttcc accattagac
    21481 aagctttgca gccgattttt cgggggaggt ggcggtttga cgagtgtcag tttcgccagg
    21541 cggcgttcca gttctttggc ttttttgaag aaggactcat tgtctccttc atgtgcccac
    21601 tgacgaaaac gacgaatcat gactttcaac cgctggactt ctttttgctg cagttcgtaa
    21661 ttttttgtca gctcagccaa gcgagcttct ttcaagactc gaaaacggct gtagtttccg
    21721 ggatactcaa tcaatgcacc ttcttccagt tcccatgttt ttgtggtgac ttgatcgaga
    21781 aattgccgat catgagaaac gaccagaacc gatttttttg tattctgtaa atagttttcc
    21841 aaccagcgaa tactgttaag atctaaatga ttggtcggtt catcgagtaa aagtaaatcg
    21901 gcatcggctg ctaaaatttt ggctaaagcg actttaactc gttcgcctcc gctaagttct
    21961 gttaaccgtg cttcctgacg gtgtggaatc cctaagcctt gcaagatcgc tgcaatccgg
    22021 tcttctaata cgtaaccgcc tagttcttca aagcgatgct gcagctcacc gtaacgggcc
    22081 agacacttct ctaagtttgt atctggttct gtcatcaatg cttcgtagcg actcatttgc
    22141 tgtttgaggt cctgtatctc agcggcagaa tggagcaagt agtggttgac ggtcgcttcg
    22201 gaaactgtaa gttcttgttc gaccgtcccg atctttaagc cttttttgcg actgataaca
    22261 ccgctgtcga caccttctct gccggtcaag atttgtaaaa aggttgattt gccagttccg
    22321 ttgataccga ctaaggcaat tttttcacca gcttcaattt gacaggataa atcctcaaat
    22381 aatggattcc cgccgattgt ttttttaata gaaattgctt gtatgatcat gatctctctc
    22441 cttgagacca ctcaaaaagg ccatggttat ccatggcctg tgttatttaa actcagatta
    22501 tcatgagaaa aataccttga gatggccatt gattttacgt gaattcaact gttttggaca
    22561 aacttgtccc gtcaacagca actgtaaaac caattgcatg gctaaatgcg atatggtatc
    22621 gttggtaatt ttcattactt gttattttag tcatgcgcat catagtattt tccctccttc
    22681 gtgtagttgt cttttccagt gtacctgaaa gtgtcagaaa gttcaactag cgctgacatt
    22741 tacttaatat ttatcgatgt gtaattctgt cagagagctt tttttatctg actgagggta
    22801 atgtctcgtg gaccacatca ttgtattctg agctaaacca gcgttcaggc gaaatcatat
    22861 ggtgtttgat aaaaaacttt gtctacatga cttccttttg ccatttgctt aataccgttg
    22921 cattaaacag ctcttctctt tggaaatctc accaacatcg cgttcaggag tatcgttgtg
    22981 gttgcaatgg caagcggtag cctttcatca attcttgaat gatttaccga atgtagcact
    23041 tgccttctcg cggtacccga acccgaaata aaattttagt ttggcgataa aatcggtatc
    23101 cgccatcgat acttcatatt ttttcgtatc agattcatcg gaaacttcca cattgacgaa
    23161 ccaataagct ttggcatgct taggtcgttt taaaccacac cagggcgact aatcgccctg
    23221 gtgttcactt ttttttattt tacttgaact cattgactgt tttcccttta ttttaaacat
    23281 tataaagacc gatgagtgct tgccgcgtct tttgttttta tcacggtttt ttgtgcaaaa
    23341 aaacctgccg atagttggca ggtcgtctac gtgttaagct ttggtgtggt ctttagctgg
    23401 caattggttt tctaaatcag ttcggacaac aagcacatcg caagctgcgt ttctaataac
    23461 atattcagaa acagaaccaa taaataaacg ttctacagca ttcaatcctg tggctcctaa
    23521 cataatcaaa tctacttgat tatcttctgg gatttctctg gcaataattt gttttggtga
    23581 gccatattct acgacagagg ttacattatt caagccagct ttcttcgcat tgctttcata
    23641 atctgccaat gtttgctttg ccatttcagt agcttgttct gcaagcattc catcaaagga
    23701 tgaaacggtt tggaaagaac gtgtgtcaat gacatgtgct aatagaagtt ctccgttatt
    23761 tcggatagct acattgaccg cctttttaaa agctaattca gcttcatcag aaccatctac
    23821 ggcaaccatg atttttttgt attgttgtaa cataagcatt cactcctcaa gtcaatcgta
    23881 ttgtactctt ttattgtaga acactttagg gaaaaagaga attatttact gctttggttc
    23941 agcaattttt tttgcaaatc ggatactgaa ggtggcagaa acaataatta cgatcagtaa
    24001 aaagaccaag gcaataaagc gttgattgaa aaaagctgtt aaaataccca ttacgcctaa
    24061 aacagcatat gcagctccgt aaaattgtag gaatccttga tttttatcat ttttgttgat
    24121 caatacaaaa aagatatcct gtttattaaa taaataatag ctaacgaata ataatactaa
    24181 agtggcgata atcattaaaa tcctaatcat gaacaaatcc tccaaaataa gcaacaaata
    24241 gaaaaagcaa tttgccaaga gcaaattgct ttattcttta aaagatggta atctccgtta
    24301 atgccgttgt ttgtcctgta gtattgatcc cgcgaattca agcaggtggg tcccatggtc
    24361 gtagcttcga actaccaaat gataaggcat gttaccagca gtgacacaag acgggtccca
    24421 agatatcaat aaaaatgttc ggtcaacaca caaagaggac aattcgaaca tcacagattt
    24481 cccacctcta tagtagcata tgaatttaaa aagagcaatg agagagctcg cagaaacaga
    24541 accgttttct tgtctagttt tttggaggat gctttttcca ctcttttata cggtaatact
    24601 gttgacccag agcctgttca tatttccctg tatcttttgg ctgataatac tgagcattct
    24661 tcagtttatc tggtaaatat tgctgattga cccaagcctg atcaaaatga tgaggatact
    24721 gataacccac gccacggttg agtgatttcg cacctttgta atggctatct ctcaaatgat
    24781 caggaacatc tccagctttg ccctctcgaa tatcagcaag tgctgcatct aatgccatgt
    24841 aggcagaatt ggattttggc gacaagcaaa gatcgacgac gacgtctgct agtgggatac
    24901 gtgcctcagg caatcccaat ttttcagcag ctaatacagc gttcactgta cgagccgcag
    24961 cagcaggatt acctaaacct atatcttcat aaccgataac catcagacgc cggcaaatac
    25021 ttgccagatc acctgcctca actaaacggg cgagataatg aagtgcagca tccacatcac
    25081 ttccacgaat cgatttttga aaagccgaaa taacgtcata atgagcatct ccatttttgt
    25141 catgggtaag agcttttctt tggacacatt cttcaatgat cgacagtgta agatgaatcc
    25201 ttccttcttt atctgaagga gtcgatagtg tagccagttc cagcccattc aatgcactcc
    25261 gcaaatcgcc gttcgttgca cgcgaaaggt gaagaagagc atcttcgtct aactggattg
    25321 cttgttgtcc aaggccgcgt tctttatccc ttaaagcatg ttcgactgcc aactggatgt
    25381 cttgttctgt taatggtttg acttcaaaga tctgtgtacg gctgcggatc gctggattga
    25441 tggtaatata aggattctca gtagtcgctc cgatcaagat gattcggcca ctttctaaat
    25501 gaggaagcag aaaatcctgt tttgttttat ctaatcggtg tacttcatcc aataagagga
    25561 tcactgtacc actcattttt gcttcttccg ctacaacttg tagatccttt tttgaatctg
    25621 tagcggcgtt tagcatgcga aatgcataat tagtagagcc ggcgatcgca cttgcgatac
    25681 tcgtttttcc agtaccagga ggtccatata gaatcatgga agagagcatg cgagcatcga
    25741 ccatgcggcg tatgattttt ccttccccga ctagatgctg ctgaccaacg acttcatcga
    25801 tcgttcgagg tctcatgcga taagccaatg gctgttgcat aggattttcc tccttcttgt
    25861 ttttacgtcc cttaattata tcatatttag caagatgcct agaaagaaca gccttctgtt
    25921 gttttgtttt catagcttat ccaactaata ttgtgtatga taaagtaggt gagaaaaatg
    25981 acggataaac aaaagataga tgaatttttt tggaataaaa cgacgccaga agtggcacgg
    26041 gatttattgg ggatgtattt ggagcatgac acaccagaag gaagattagc tggatatatt
    26101 gtagacgcgg aagcatacct aggtccagaa gacgaggccg cccatagcta tggtttaaga
    26161 agaacaccgc gtgtgcgggc aatgtatgaa aaaccaggta cgatctacct ttacacgatg
    26221 catactcatc ggattttgaa catcattact cagccagaag ggattcctca aggagtaatg
    26281 atacgtgcaa tcgaacccgc cgcaatgatt gatcaaatga gtaaaaatcg tggcggaaaa
    26341 actggtccag atatcagcaa cggtccggga aaattagtgg aagcattagc aattcctcaa
    26401 gaactttatg gtcagtctat agcagatagt tcgttacgct tagtttttga gaaaaaaaag
    26461 acaccaaaaa agatcaatgc tttgcctaga atcggtatcc caaacaaagg tgtatggaca
    26521 gaaaagccgt tgcgttttgt tgtgtcggga aatccttata tctctttaca aagaaaaaac
    26581 caaatcgaga aagattgggg ttggaggaaa gaaaatgaaa aagaaggatc tattaacata
    26641 tttagatgaa ataattgaaa aacaagtgac agactatgat gtagctttag attgggatac
    26701 aaaaaatcat acgatcgaga tcgttgttcg cttgtttgca gaaaataaag cacaatttga
    26761 gattgatgat gcagaaggga tcgtttcgca agaaccaatt atcgaatttg aagacggcgt
    26821 acttcttttt aatcctcaga aatctgtttt tgatgaacgc aattatctag cagtaatccc
    26881 ttatgaagga aaaaaagggt tggcaaaatc agtagctgat tctttagtgg aatatctaaa
    26941 tgaagtatta gctcaagggc aaagcgattt gcttgatttt ttggatgaag acgatgacga
    27001 agctgtattc gagcttcact gggataatca gcaactagca gaattagtag aagtaaaaca
    27061 gcaaaatgaa gcagatactt atttaccgta cccaagttat taaggagtga cagatatgaa
    27121 atggaatgaa gtaaaagtag aaactgaaag tgaagcagtc gaagcagttg ctaatatttt
    27181 gatggaggca ggggcgagtg gtgtcgcgat tgaagattct ttagatgttg aacattttca
    27241 gtcggatcaa tatggtgaga ttttagataa aaatgatttt acatcgctga aagaaggcgc
    27301 actagtcatg gcttattttc cggaaacgac tttcttaccg gaaatcttgc cttttatcaa
    27361 agatcgtatc acacaattac ctgagtttgg tctagctatt ggaaaaaata aagtaacagt
    27421 tagtgaagta gaggaaagca actgggcaac agcctggaag aaatactatc atccggtgcg
    27481 tattacgcga atgctgacgg ttgcgccaag ctgggaaaac taccagacag aagatcctct
    27541 agaaaaaatt atcacactag atcctggtat ggcatttggc acaggcactc atcccacgac
    27601 ttccttgaca ttgacagcac tagaaacagt cttgcgtggt ggtgaaacat tgctagatgt
    27661 gggaactggg tcgggcgtgc taagtattgc agcaaaatat cttggtgcaa aagatgtcta
    27721 tgcctttgat ttagacgaag tagccgttcg ttcggcgaaa gaaaatatgg atatgaatga
    27781 aatagccaaa gatgtccatg tatcagctaa cgatcttttg acaggaatcg agattgaaag
    27841 tgatgtaatc gttgcgaaca ttttagcaga tatcatctta ttgatgatcc cagatgcttg
    27901 gcgtttactg aagcaaacag gcacgctgat cgtttcagga atcattgaag agaagaagca
    27961 aatggttctg gatgccatgt tagaacaagg gtttatcgtg gatcaaattt tccagcaaaa
    28021 agactggtat gcaattattt tgaaaaaacc agaggtggaa tagatgcagc ggtatttttt
    28081 aaaagaatca tatgaatcaa aagactattt tgttttagaa ggggaaaact atcaccacat
    28141 tgtacgggtt atgaggatgg aacccgagga tgaagttttt ttggcattca atgatcaaat
    28201 agccattgtt gcagaaatca cggagattac tgaagaggct gtttatctga aagaaattaa
    28261 aaaagaagaa aatcaaaagg aactgccaag ttcgattacc atagcgagtg gctatcctaa
    28321 aggggaaaag ctggattgga tcgttcagaa atccactgaa ttaggagcgc aagcttttct
    28381 tggtttcccg gcatcagcct ctgtcgttaa atgggatgca aaaaaacgta tgaaacgaca
    28441 acaacggctt gaaaagattg ctcaagaggc agcggaacaa tcccatcggc agatcgtgcc
    28501 atcagtagag ttattggaaa cgaaacaaga tttatttgag cggctagatg attatgacgt
    28561 ggtcttggtt gcttatgagg aatcagcaaa gcaaggcgaa aaatcaaatg cagccaaaat
    28621 tcttagtgaa acaaaaccag atagtcgaat cttagctgtc tttggaccag aaggtggatt
    28681 atcccgcgat gaagtcgatt attttgtaga acacaaaggg atactttgtg gattaggacc
    28741 aagaatcctt cgtacagaaa cagcacctct ttatttatta agtgctgcta gctatcaatg
    28801 ggaattaatg aatccttctt tcagctaacg ttcttcatta ataaaagagt atcgacagaa
    28861 agaattgaaa aaatagtcaa caatttgtat aatagaaaca attatttata caatggatac
    28921 gaaaaatcgt ttgaaaaagc ttttaaaaag cgaacatttc cgtaaaaaaa cgtataactg
    28981 cacggatttt gggacaatta tcaaacagat atttgtcccg attcccgttt taccgaaaaa
    29041 agaaaggggg atcgattcct gtgacaaaag aagaaatttt aacgggaccc ggtgtcataa
    29101 agctcgttag tcaatacatg ggtcccgaac atgtagcatt tgttgagaaa gcatgcgaat
    29161 acgcgactgc tgcacatgac ggacagttta gaaaatcagg cgaaccctac attattcatc
    29221 ctatccaagt cgcaggtata ttagcagatt taaaaatgga tccccataca gtggctacag
    29281 gcttcttaca tgatgttgtt gaagatacag aaatcacttt agaagatctg agagaagaat
    29341 ttggcgatga cgttgctatg ttagtggacg gcgtaaccaa attagggaaa ataaaatata
    29401 aatcccacga agagcagcta gcagaaaacc accgaaagat gctgctagca atggctcaag
    29461 atttacgagt tatcatggtc aaattagctg atagactaca taacatgcgt acgttgaagc
    29521 acctgcgaga agataagcaa agaaggatcg ctcaggaaac tttagaaatc tatgcgcctc
    29581 ttgcacatcg tctagggatc agccggatca aatgggaatt agaagataca gcacttcgtt
    29641 atctaaatcc aaaacagtat taccgtatcg tccacttgat gcagacgaag agagaagaac
    29701 gtgaaaaata cgtaagtggt actgttgaag atattcgaat agcgacagaa gagctgggga
    29761 tttttgcaga aatctatgga cggccaaaac acatttattc aatctatcgt aaaatgaaag
    29821 atcagaaaaa gcagttcaac gaaatttatg acttgctagc tattcgagtg atcgtagatt
    29881 caatcaagga ttgttatgct gtattaggag caatccacac aaaatggaaa ccaatgcccg
    29941 gcagatttaa ggattatatt gccatgccaa aagctaatat gtaccaatct ttgcatacta
    30001 ccgtgatcgg tccggcagga aatccggtag aaattcaaat ccgaacacag gaaatgcatg
    30061 aaatcgctga attcggggtt gctgcacact gggcctataa agaaggaaaa aatgaaaaag
    30121 tagaaccaga tggtatgacg aaacaattaa gctggttcca tgagatactc gaacttcaag
    30181 acgaaagcta tgatgcttct gaatttatgg aaggcgtaaa aggagatatc tttagtgata
    30241 aagtctacgt cttcacacca aaaggagacg ttactgagtt accaaaagga tccggaccat
    30301 tggactttgc atacagtatc catacagata tcggtaacaa aaccactggt gcaaaagtaa
    30361 atggcaaaat ggtgcagctt gattacaaat tgaaaaacgg agatatcatt gagatcatga
    30421 cttctccaaa ttcatttggc ccaagtcgcg actggttgaa attagttgct actagcaaag
    30481 caagaaataa gatcaaacgt ttcttcaaag cccaagatcg agaagaaaat gtgatcaaag
    30541 gccacgaatc cgtggtcaaa tgtattacag atctaggatt tacgcctaaa gatattttga
    30601 cgaagaacaa actgcaagaa gcactcgatc gttttaatta tcaaacagaa gatgatctct
    30661 atgcagctgt agggtatgga gaagttagcc ccttgacgat ggccaatcgt ctgactgaaa
    30721 aagaacgtaa agaacaaaaa atcgagcagc aaaagcaaga agcagaagaa atcatgaatc
    30781 agccgaaaaa agaacctgac aaaatgaaag tacgtcatga aggtggcgtt gtcattcaag
    30841 gggtagaaaa cttactaatt cgtatcagtc gctgctgtaa tccgattcct ggtgatgata
    30901 tcgttggtta tatcactaaa ggcagaggga tatccattca tcgtcgagat tgtccgaatg
    30961 ttcagcctga caaaccaaat gtagcagaac gtttgattga agtcgaatgg gaagatacat
    31021 cgaatacacg aaaagagtat gatgcagatt tggaaattta cggctataat cgttcaggct
    31081 tattgaatga tgtacttcaa acagtcaatg cgctaacgaa aaatctcaac agcgttgaag
    31141 cacggacgaa taaagataaa atggcgacga tccatttgac ggttggtatc cagaatttat
    31201 cccacctaaa gagtatcgtg gataaaatca aagcagtacc tgatgtctac agtgtacgcc
    31261 ggacgaatgg ataggaggac agatatgaga gcagttgttc aacgggtaag taaagctgct
    31321 gtgtcagtcg atcagcaaga aattggaaaa attggctacg gcctacttgt tttattaggc
    31381 attcatgaaa ccgatacgca aaaagatgtg gactatctgg taaaaaaaat tgcacaaatg
    31441 cgtatttttg aagatgaaca aggaaaaatg aatattagtg tagaagatgt gggaggacaa
    31501 attttaagta tctctcaatt cacactgttt gcggatacga aaaaaggaaa tcgaccaagc
    31561 tttgtggaag ccgcacgacc agaaacagct attcctttat acgaatcatt caatgaagga
    31621 atcagaagtc gcggcatcac agtcgctacc ggacaattcg gagcagatat gtcggtttct
    31681 ctcgtcaatg atggacctgt aacgatcatt attgattcta aaaacaaata aagtaatctg
    31741 gttttcacta gtaaaaacta tgaaacacgt cgcttaagtt agactgataa actctaactt
    31801 aagtggcttt tttgtctgca aaaagcgctt cttttgtcag cagtcggaaa aaaagttaag
    31861 cttaaattat acaagttatt ttttatagga ggttgctgaa gatgaaagaa gtaccagtag
    31921 tagatatcaa aatcacagat ttaaaaaaat tgtatcaaaa aggatacaac atgttggagg
    31981 agttacgcca tgaagcggac gctcccgtcg tcaaagcaaa gattttcaat aaagaagcta
    32041 tcacgatcta cggatcatca gcagcaaaag tattttatga tccaaggaat ttcaaacgaa
    32101 aaggtgctat gccaaaatta gtattgaaga cactttttgg tcaaggtgga gtacagactt
    32161 tggatggagc tgctcatcac catcggaaaa atatttttat ggatctaatg acaccggaaa
    32221 gaatggaaga ttatcatcgt atcttagata agaatctgac acaagcactt gaagcacagc
    32281 atggtcaatt tgaactattt gatttaagta agatggtttt ctttacatct atttgcgagt
    32341 gggcaggtat caatttatca gcgatttcta aagatgaggt cgaaaaatta gcagaatatc
    32401 aaatctctat gatcagcgga acgtttactt ctccgattga tcacataaaa ggtgtcgaaa
    32461 atcgcaaaaa atcagaaaaa tgggcgcaag gcttgattga agaagctcga caaaatcctg
    32521 tggctggaaa agaaaatgtg gcattatatg cttttgccaa tgccacagat ttagatggtc
    32581 agcttttacc tctggaagtg gcagctgttg aattattgaa catcatccgt ccaacggttg
    32641 ctctaactgt gtgggcggca ttgatgggac atgcactttt cagtagacca gatttgtatc
    32701 agcaattaaa aaatgacttt tcgacgttac aagatccgtt tattcaagaa atgagacgct
    32761 attatccttt cttcccaatg ctacctgcga tttcgctgaa agaagtagaa gtagatggtt
    32821 atcgtatacc agaaggcagt tgggttattt tagatttata tggaacagac catgatgaac
    32881 gaacagttga agcgccagat tcctttatga tcaaacggta tgtagggaaa gcaaaagata
    32941 tttcctataa agaagaatat gagatgattg cacaaggcgg gggaaatttt cgacaaatgc
    33001 atcgctgtgc aggagaatgg atcactcttc atagtctacg tgtgttttcc gatcagctag
    33061 taaataagtt tgagttctct gtacctgaac aagattggac gattccattc aatcagtttc
    33121 cgacttatcc gaatagccga gcgctacttt acaagaatta gattagcata aacttatcga
    33181 aaaaaaggaa gatcaaacag tgctgatctt ccttttttca tattactata aattgataag
    33241 gtcaattgat caaaatccac gaaatgcttc atcaatggtt tgataatctt cattggataa
    33301 atgcaaatct aaagcttgga cattgtcaga tacttgctct ggacgcttag ctccaggtat
    33361 aactacagac actcgtggat tcttcatgta ccaagctaaa acaagttgcg caggagtggc
    33421 ttggtatctt ttagcaatgg ggcgaagtac atcgacagcg gttaaaattt ctctgaaacg
    33481 ttctccttgg aaatctggat ttctttttcg aggatctcct tcaccaaatt gtttttcctc
    33541 accaagttca tattttcctg tcagcaaacc ggaagcaaga ggaaaatagg gaacaaaact
    33601 gattttgttt ttttcaagat aagggaataa ttctttttca gcctgccgat ggatcaaact
    33661 gtatttatct tctactacat cgacatatcc atctgcattc gcttctttca attgttctaa
    33721 cgtgaagttt gacacaccga ctgctcggat ttttcctgct tcttttaatt cgtgcaatgt
    33781 agcaactgat tcattttttg gtgtgctttc atccggaaag tggatataaa aaatgtcgat
    33841 gtaatcagtt tgcagacgtt ttagtgcatc ttccaccgat tgtttgagaa actcaggaga
    33901 attatcaaat gttcgccctt tatttggtac atgagcagct tttgtagcga tgacgacacg
    33961 tgagcgatca tattcttttt ccttcagtac ctcgccaatc agttcttcgg aacggccgtt
    34021 tccgtaagca taagcggtat cgatcaaatt gatcccgcta ttcaaagctg tacgaactac
    34081 ttgttttcct gtctcatctt ccaaaccgtc aaaaagatta tgtcctccaa cggcatttgc
    34141 tcctaatcca agctgttctg cgtagacttg tgaatgtccg attcttattt ctgccatagc
    34201 tgattcctcc tttttgtcta gtctaaaaca aaataccaaa aagctgaact aaaacgtttt
    34261 tgtgagggga aatagtcaga tatctcttga ctttgagact ggtttttagt aggttataag
    34321 taatatcgaa tgtcaatgaa agagcgagta agtttttgaa actttacaag agaggatcat
    34381 cgaggctgaa aatgatctaa agtttcatta gcctaaagac actctggaga tggacgagtg
    34441 aaaagccgtc ccgcgtacga gcgttattcg taagaggaaa gagaaatctt tcaaaattag
    34501 gtggtaccgt gtatgaattt cattcaccct tgttcatgat gaacaagggt gattttttta
    34561 tttacatagc taagaaaggg agagaaaact atgagtttcc aaagaccaaa aggaacaaat
    34621 gatattttgc caggcgaatc agaaaaatgg cagtttgtcg aagaaacagc acgtttatta
    34681 ttcagagatt atcagtataa tgaaatgcgt acaccgattt ttgaacacta tgaagtgata
    34741 tcccgaagtg taggggatac aacggatatc gtttcaaaag agatgtatga tttttatgac
    34801 aaaggggaac gccatgtgac gcttcgacca gaaggtacag caccaatcgt tcgttctttt
    34861 gttgagcata aattgttcgg cccagagtat gcgaaaccgt tcaaaacttt ttatatggga
    34921 ccaatgtttc gctacgaacg ccctcaaaaa ggacggttac gtcaattcca tcaaatcgga
    34981 gtagaagcat ttggatcaga aaaccctgca actgatgtgg agagcatggt gatggcactt
    35041 gatttcttca aacagttggg gatcacgcaa atccggctag tcatcaattc attaggtgat
    35101 aaagaaacgc gtcaagccta tcgtcaagca ttgatcgact atcttttacc ttttgaatca
    35161 gagctgagtg aagactctaa acgccgcctt catgaaaatc cattacgcgt attggacagt
    35221 aaagacaaac gagatcaaaa atttgtggca gaagctccat ctatcttaga ttatctaagc
    35281 gagcctgctc aaaaacattt tgataccgtt gtttccatgc tggatgcatt ggctgttcct
    35341 tatgaaatcg acagtaatat ggttcgcgga ctagattatt acacacatac gatttttgaa
    35401 atcatgagtg acgcaccaaa aatgggggca caagcaacga tttgcgcagg cggacgttat
    35461 gacaatctag tggaagaact aggtggtcct tcaacacctg gcttcggatt tgcaatgggt
    35521 atcgagcgat tactcttgac tatggaggca gaggaagtag ttatccctgc atttaatgag
    35581 ttggatgctt acgtggtcgc actcggagac gaaacaaata tcgaagcatt gaaagtggtc
    35641 caagcgattc gaaactttgg tttttcagct gaccgcgact tcatgaaccg taaagcaaaa
    35701 gcgcaattta agacagcaga taaagcgaat gccaaattag tattggtgat cggtgaagat
    35761 gagcttgcta gtcaaacgat taatgtcaaa gcaatggcaa gccgaaaaga aaaaagtttt
    35821 ccattagaag agatatatga acattttgat gaagtctatg atgaaatgac tttattcgag
    35881 taggaggaaa aaaataaatg gcacaaagaa cgatctattg cggattagtt tccgaacaac
    35941 aattagaaca aacagttaca ttaaaaggat gggtacaaaa aagacgagac cttgggggag
    36001 taatctttat cgacctacgt gatcgtgaag gaatcgttca aatcgtcttt aatcctgaaa
    36061 tcaacaaaga agcatgggaa atcgcggata aatgtcgcag tgaatacgtg atcgaagtga
    36121 ctggggaagt tagaagacgc ggagaaaatg cagtaaatcc taaaatggcc actggtgaat
    36181 tcgaagtgat tgcaagtgaa atcgtgattt tgaatcaagc aaaaaccccg ccatttcaaa
    36241 ttgaagatga tcaagcaatc agtgacgaaa tacgtttgaa atatcgttat ttggatctgc
    36301 gtcgtccaaa aatgactaaa aacatcaaat taagaaatgc gacaacaaaa gcgattcgtc
    36361 attatttaga tgataatgac tttctcgata tcgaaacacc ttacctaggg aaatctacac
    36421 cagaaggcgc aagagactac ctagttcctt cacgtgtcca tgctggacat ttctatgcgt
    36481 tgccgcaatc tcctcaattg tttaaacaat tgctgatgaa tgctggattc gaccgttatt
    36541 atcaaatcgt tcgttgtttc cgcgatgaag atttacgtgg agaccgtcag cctgaattta
    36601 cgcaagtaga tattgaaaca acgttcttaa cagcagaaga aatccaaact tacacagagg
    36661 gattgattgc gaaggtaatg aaagaagttc gcggtatcga agtcacattg ccatttcctc
    36721 gtatgaccta tgatgaagca atggctcgct acggcagcga caaaccggat acacgttttg
    36781 atatggaatt gatcgatcta agcgatacag tcaaagaagt agaattcaaa gtcttccaaa
    36841 tggctcttga aaacggcggt gtcgtgaaag cattgaatgc taaaggcgct gcagatcgct
    36901 actcacgaaa agacatggat cagttaggtc aatatgtagg acaattcgga gctaaggggc
    36961 ttgcatggct gaaagtagaa gaagacggat tgaaaggtcc aattgctaaa ttcatgggag
    37021 aagcaacaga agcaatcatt aaagcaacag atgcaaaacc aggtgacttg ctgatgtttg
    37081 gcgcagataa aagtgaaatc gtcgctgctg cattaggtgc tatccggaca cgtttaggaa
    37141 aagaattagg actaattgat gaatcgaaat tcaatttctt atgggtaact gattggccac
    37201 aatttgaatt ttcagaagaa gaaggtcgat atgtctctgc tcaccatcca ttcacgatgc
    37261 caaaagaaga agatatcccg ttattggctg aagaccctgc aaaagtctat gcagaagctt
    37321 atgatatcgt cttgaacggt tatgaactag gcggaggttc tatccgtatt catactcgtg
    37381 agcttcaaga aaaagtacta gaaactctag gctttacaaa agaacaaatg gaagaacaat
    37441 ttggtttctt gttaacagct ttagattacg gattccctcc acatggcggt attgcattag
    37501 gattggaccg ttttgcaatg ttattagccg gagaagataa tattcgtgaa gtcattgcct
    37561 ttcctaaaaa cggaaaagca gcagatgtga tgagtgatgc accaagtatg gtttcgcctt
    37621 tacaattgtt cgaattgaat attgatgtaa cgaatataga agaatagccg ataaaaaggt
    37681 aaaaaggatt tgtcgaatac gcttcttttg acaaccttga tttaattgaa aaacacttta
    37741 taatgaagcc ataacatata aagcggggct tttccatgaa aaaaacattc gaaaattggg
    37801 cttatcatga ttacacgacc aagctttcgg tatctatcgt ttatgcgttg atcgcatcag
    37861 tcgcattgaa tttcttttat cagccaggac atatttattc aagtggtatt actggtctag
    37921 ctcagatttt gacgactttg tcaaaatcac tgataggatt cgaagtaccg gtatcaacaa
    37981 ctttgtattt actgaatata cctttgtttg tattggcgtg gttaaaaatc gggaagaagt
    38041 ttacaatttt cacttttatc acagtcactt tgacctccct ttgtatccat attttaccgg
    38101 aaacagtcct atctaccgat ccaatcatct gtgcgttgtt tgggggggct gtgatgggac
    38161 taggtattgg gctagcgttg agaaacggcc tatcatcggg cggtctagat atcgtgagta
    38221 tcacgattcg taaaaaaaca ggaaagtcga ttggttctat ttcgatctac ttcaatgcat
    38281 tgatcgtttt tgttgccgga attctatttg gctggcaata tatgttttac agtacgttgt
    38341 ctattttcgt cagtggaaaa gtaactgatg tcgtttatac taaacaaaag aagatgcagg
    38401 taatgatcgt aacgaaacat ccagaaaaag tagtaaatga aatccaaaac tgtatgcgta
    38461 gagggatcac gatcatcaat gaagcagaag gtgcttataa gcatgataag cagacagtat
    38521 tgatgacgat cgttacacga tacgaactgc ctctacttag agaagcaatg aaagcttctg
    38581 atccacaagc ttttgtaagt attgcagaaa acgtccagat acttggtcgt ttctatgagg
    38641 atggcattta gatcgagttt gacaaaaatt catgaaatac tgctgtagac agacgttcgt
    38701 ttgtctgcag tttttttcta ccatagtaga atcagcaaag aaaaactgct tttttatttg
    38761 aaattttctt aaagttgaaa aaaagcatgt ttattttttt aaatttatgg gatgatagaa
    38821 aaaaatagta gagaaaggac acgattctac ttgctatttt cacttaatac ccgtatatta
    38881 cgaatgaaaa aagtttatga aaagttggaa tattgaatgg aaaaacaaaa aaggttaaaa
    38941 aacagccgct acatcactgg gtttgatggg ctccgttcat tagctgtggt gggtgttatc
    39001 ttctatcacc ttctgccaac tagtatgaaa ggcggctatt tgggcgttcc gatttttttc
    39061 gtcgtatctg gttatttgat cacggatctg ctgcggcagg aatgggaaca aaatggaaaa
    39121 atcaatattt ggcaatttta tgtcagaaga atgaagagac tctatccggc acttgttttt
    39181 atgcttgtga cagcttcggc ttatatcact ttgtttcagc gagggctgtt aaacaatctt
    39241 cgaggagttg tggtcagcag cttactgtat gtcaacaact ggtggcaaat caataacggt
    39301 ttatcttatt tcgatcgatt tgctaatgag tcaccgttta cgcatatctg gtctttagca
    39361 gtagaaggtc aaaactattt ggtttggccg atcttgtttg tattgttgat ggtatttgtt
    39421 cgtaaaagaa aatggatctt ttatactgtg ttaggtacat ccttcctctc cgccatcttg
    39481 atgatgatct tgtatacacc aggaggcgat ccaacccgtg tttattacgg taccgacact
    39541 cgtctgtttt ctatatggtt gggaagtgcg cttgcgtttg tttggccaag tacgcgttta
    39601 aagaaaaaca tcccgaaaca agcgaaaaat attctgaata tcgctggagg aatctcgctt
    39661 ttggcactga ttctttgctt cttcttttta gatgatcatt acaatttcat ctattacgga
    39721 ggaatgctcc tagtgagtct gttatgcgta gtgctagttg cagtgacagc acaccccgga
    39781 gcaagtttga atcgatggct gacaaatcca gtatttactt ggattggcaa acgaagctat
    39841 ggcatttatc tgtatcagtt tccagttatg attttttatg aagcaaaggt gactgatatt
    39901 gccgatcatg ttttgttgca tactttagtg gaaatcacat tgattctaat catcagtgaa
    39961 ctttcttatc gatatgtgga gcgtccgcta gcacgttttg attatggtcg tacattccaa
    40021 gtgatgaaag gatggttcag caaaccgatc atcagtaaga gcaaaccatg ggttgttcca
    40081 ggcacattac ttgtagcaat tgcactcgtt ggtttcgtta cggcaccgaa aaacgctgta
    40141 acagccgatc aaaaacaact gcaggcaaaa atcgctgaaa gtaaaaaatt agcagaagaa
    40201 accagaaaca attcagaaaa caccactagt tcggttgacc aagcgatcct tgataagtac
    40261 gagttgtcca aggcacaagg gaataaggca caaaaattag aactaacggc atttggtgat
    40321 tcagttatgt tggatgctgc aacggatcta caagaacttt atccaaaagt agtggtagat
    40381 ggagatgtcg gacgacaatt atatgccagt gtaccgtaca ttgaaaaatt aaaggaacaa
    40441 gatttgttga aagacactgt tctgatcgga ctaggaacga atggctcatt ctcggaagct
    40501 cagtttgata gtgtgatgga tgcaatcggt gatcgaaaag tatactgggt caatgtccga
    40561 gtaccgacga agcgctggca aaatgacgtg aatgcgatgc ttgaacagat gacgaaaaaa
    40621 tacgacaata tgacgctgat tgattggtac agctacagta atgaccatag tgactggttt
    40681 tatgatgatc aagtccatcc aaatcctgaa gggatgacac attacattca cttagtcagc
    40741 gaagccttgc taggaggaag ttcaagtacg aacagcagtg agacaaacgt gacagaatga
    40801 tagctacaaa aaacttcagg caaaaacatt tgtttttgcc tgaagttttt ttatatttac
    40861 cgtgttttta acattcatat agattcagga agcaaaacgg ttgagtgtgt ctgtaaataa
    40921 cgttcttttt gttttcgggt gagttttttg aatgcagctt ctgcttttgt tgcttcgctt
    40981 cttgaagaaa atacttctgc atggatcatc ttaacaggtc ttcttttttg aagacgagta
    41041 tatttagcac cagtaccatt gttatgttcc tctaatcgtc tagaaagatc ggttgtatat
    41101 ccaccataaa atgtctgatc tttacagaat aatacgtaaa aataatgttc atttgccata
    41161 gagcatctca cgaatctctg cagtgtattc tccatccata ttgtaagtga tcaatggtgg
    41221 agcgactttg aatccgtcta atttgccttc tttgattcct tcaactaaca aaatatttgc
    41281 ctcttttcca ggttttgggt aaacaaattg gatcctttta ggtgcaattc gatagtcttc
    41341 catgacctga atgatttcta agaaccgatc aggacgatgg accatagtaa agcgtccatt
    41401 tgtttttagt agcttacttg aaatatcgat cacttcttct aacgtcgtat gaatctcatg
    41461 acgggcgatt gccaggtgtt tattagggtt cgttttattt gttggcagcc ctttgaaata
    41521 aggtggatta cagacgacta agtcacaaga attatgtcga acgacagata agctgtcttt
    41581 cagatcgatg gtgtggacag tcatttgttc atttaaatgg ttaagcgcga tactacgtct
    41641 tgccatgtct gctagacgag gttgaagttc gatagcgtcg atagccgcct gggttcgttt
    41701 acttaaaaaa agtccaactg cgccatttcc tgcacaaagg tcaacaatct tccctttttt
    41761 aggtacacgg gaaaagtgag caagcagcac agcatctaaa gaaaaagaaa acactttgga
    41821 actttggata atcttgatat cttctgcgta taattggtcg attcgttcgt cttcgtgaag
    41881 catggattcc ctccaaaatg aaaaaaatag tcgtctgtat tatactattt ttattaaaaa
    41941 gaaccaaggt ggcttgtcaa acctctattt tttatgcata ctatgaccat aagaagggaa
    42001 aggaagagac tatgttttat cgatttatgc gcggggtagt tcgagtacta ttagccttag
    42061 ttaatggaaa tgcccgctat gagaataaag aagttttacc gcaagatgag aattacatac
    42121 tagttgctcc ccatcgtact tggtgggacc cattatatct ggcggtagct gcagcaccca
    42181 aggaattcag ctttatggca aaagaagaac tgttcaaaaa tccagttctg cgatttatat
    42241 taactcgatc caatgctttt ccagttaaaa gagaaaatcc aggaccaagc gtcattaaaa
    42301 cacctgtaaa gattttgaaa tcgacaaatt tgagtttgat catgtttccc agtgggacaa
    42361 gacatgctac agaacttaaa ggcggaatgg cattgatttc cagaatgtct aaagcgaaaa
    42421 tcgtgccagc tgtatatcaa ggtccgttga cacttgggga tctgttcaag cgtcagagag
    42481 tgacagtgcg tttcggcgaa ccaatcgact tatcagatat caagaaaacg gataaagaag
    42541 gtatggcgga gatcgaacga cgaacacaag cagcttttga caaattggac aaagaagtga
    42601 accctgattt caaatatgaa gtcaaaaaaa aataaaatga taagtttatg cgtgggcggt
    42661 cttttgaatc tactgtcctg ttcagttcag actttttcaa tgaaaacgag ggagagacgt
    42721 caagtctgcc ctctttttgc gttccaatat gtaaaataga agacaggtgt catgtaatgg
    42781 atcaaagtgt tataataatt agaaggacat tttttgaggt gattgaatgc gttttttaca
    42841 tacagccgat tggcatatcg gaaaaaaact acaaggttat gacttgattg aagaccagcg
    42901 tcatgtacta tcttctatac tagatatcgc gaaagaagaa aaagtagagg caatcgtgat
    42961 tgccggagac ttatatgata ggagtgtccc agctgttgat gccgtagaat tatacaatga
    43021 attgatggtc gattggaatt taaaggaaaa gatcccgatt tttgctatat caggaaatca
    43081 cgatagcagt acgagacttt cagcgggtac gccatggttc agttattcga actatttttt
    43141 gcgaacacag ctagcagaag catttgaacc agtcgagttt aaaaacgtac aatttttctt
    43201 tcttccttac tttgaaccaa ttgcagcgag attatatttt gaagatgaca atatccgaac
    43261 gatcaagcaa gcggtagcca aggtgataga taaaatgcag gagctatttg attcagaaaa
    43321 aaaacaagta ttgattagcc atttttttgt aatgggaagt gtaaaatctg attcggaaac
    43381 gaaaatcgaa gtaggcgggt tggattcgat ccctggtgaa ctacttacac cgtttgatta
    43441 tgtagcacta ggacatttgc ataatcaagc tgctttgaga caggataatg ctcgctatag
    43501 cggttcccca ttgaaattct ctttatctga gatcaaccaa aaaaaaggcg tatggatcgt
    43561 tgacctgaca acaggcctgg agttgaattt tagggagatc gaacccctgc atgatattga
    43621 agaaataaca gcgagtttca aagaattgct ggatcctgcc ttttatcagt ccgtcaatcg
    43681 ggagaattac atctatatct atctcactga tagaacgatc atcccgaata tgatgaacca
    43741 gcttcggcaa atatatcctc gtattttggg gattgaacgg gcaaacggtc gagcagaaca
    43801 aacaaagcgc aaacgaaaag tactagaaat gaaagatcct caaaaattag cagaagcatt
    43861 ttttagtgaa atgacggaag agcagctaac tgcaaaccaa gaaaaatggc tcggagaaac
    43921 gttgcaagaa atcatggaaa tggaatgatc aagaatgaaa ccgaaaaaat tacaacttaa
    43981 aaattttggt ccttttatca atgagaccgt agatttttca cgtttgacag aagcaccgct
    44041 ttttttgatc agcgggaaga caggagctgg caaaacgacg atttttgatg ggatcacctt
    44101 cgcacttttt ggtgaaactt ccggaagact tcgctctgga aaagagatgc gttctttgtt
    44161 tgctacacca gacgaagaaa caagcgtgac tttttctttt gaacatcagc agatgagcta
    44221 tgaaatcgaa cgaaaaccag aacaagtttt agcaaaacga aaaggaaacg gaacaaaaaa
    44281 acaggcagcc aaagtaacgt tgaccatttt tgatgggaaa ggaaaagaaa taaaacaaat
    44341 cacaaaacgt acagaagcag atcagttgat cagagaactg atgaatctcg atgcgaaaca
    44401 gttttctcaa atcgttttac taccccaagg agaattccgt aattttttgg tttcatcaag
    44461 cagtgataaa gagactgttt tacgtcattt attcggtact caatttttcc aacagttcaa
    44521 tgagcgatta aaagaaaaag caaaacaaca gcaacaaaaa ttggatcatt tagagcaaga
    44581 gctgcatctt ctgcaaaaaa ggtttgtttt agtcaaagaa gaggaaccag cagcagggtt
    44641 tgaagccgtt ttagcccaat ggacaaacca tcaggcaata ctcgaaaaac aaatcacgga
    44701 agaaaaagaa gctctaacgg aacttcagga aaaacaaaag caactagaaa attcctacta
    44761 tcgttttgaa agcttgaaaa aaagttatga agaaaaaacg atattaataa acaagcaaaa
    44821 ggaattagct gctcaaaaag acgaaataga ggaaaagaaa cgatggattg ggtattatga
    44881 atttacgcaa cgattagtcg aaccaatcca gcgcctacaa gaaataaaag ttgaaaaaat
    44941 agaactgtca caaaaaagta gtgagcaaac aaagaaacta gcgatgacta cgcagaatta
    45001 tgaagagtgg caaaaaaatg aagcaaaccg cgtacgccga caaacaaaaa tcaaagaagc
    45061 tgctcaaaaa ctgcaaaaca aacagatgat ggtgccaatt gtcgaatcct tacgtagtaa
    45121 aaaacaagac gcagaacaat taacaatttc attggataaa gaagaattga atgccgagaa
    45181 gctgtatcaa aatctcgcta tctatcagga aagaactcga attcttcaag aaaatttact
    45241 ggatcaggaa aaactgcaag aagatcgtct tgcttttgga cagttaaaat atctagatga
    45301 aaagcagcaa gaaatagaaa aacaaaaagc aaaagacact gggtcgctcg cacagataag
    45361 agaagaactc aaaaaagcaa ctgaagaaat aacaaaaaat agtcagctat tgaaagaaca
    45421 gacagaacaa gcagaacagc tgaaaagcca atgggcaaag cagcaaattg ctcgtcttca
    45481 gctttttttg cgtccagggg agccatgtct tgtttgtgga tcaacggaac accccataca
    45541 acaattaaaa cacgaacagc caaataagga agatatctta aaagtagaac aacaactggc
    45601 agaagctgag tctgctgtag aaagaactca gcaaatcatt gccaaaagtg aagcacatga
    45661 aaataatctt caacaacaat tatcagattt agaacaaaaa atcatggcaa cgaatataca
    45721 attggctgaa caagaagaac acttgcaaca ggctttgaaa aaggcatttc ctgagataac
    45781 agaacaaaaa acaaaaacaa ctcttgtagt aatcgaacaa tctttagata gacgaaaaag
    45841 caagatggcc aatgcaagaa tagagctgga agaaataaga caacaagcag aaaagctcga
    45901 acaacaatta gctgtctaca aacagcagca gacagaaaaa aataatcagc tccagcaact
    45961 gcaaggagaa atcaaaagtt tagaacaaca attgcaagat acggatagta actgtttaga
    46021 agaggagata cgacgacttg aaaaacagtt agaagaagac tcgaatcaac tagctgttga
    46081 gcagaaagca ggagtgcaat tgttgcaaga tcttcatacg cttcggacac agcttgatct
    46141 tcaacatgaa caaatagaaa aaatagatga aaaaatagaa aaggaacaag aaagaatagc
    46201 taaaaagtta gaaagccagt cgtatttttc aacaattgaa gaaatctgtg catttgcaca
    46261 acagcaagca caatataaag aaaaccaacg tacagttact tcttatcatg aagagcaatt
    46321 agtgacagct gaccggctgc gtcaattaga aaatatccag ctaactgaga cattcccaga
    46381 tacagaagag atcagccaac agttagaaga aataagacgt atgatcgaaa taaagcaaga
    46441 aaagatatac ggtttacaag aacaaaaaat tagtaatcaa aaaatctacg atgaatgtct
    46501 ttcgatttat cacacaagcc agacgaaatt agatgagctt acgcaattac agcagctttc
    46561 gcaaacattc aacggagaaa atcctaagaa gactagtttg gaacggtatg tgcttcaggt
    46621 ttatcttcaa gaagtgttac aagtagcaaa tgatcatctc caacgtttaa cgaaaaatcg
    46681 ttatcaattt gagctagcgg ataccattgg cagttatcga gggaaaaccg gattagaaat
    46741 caatatctat gatgatcagg caggtatgtc tcgaagtgcg cataccctgt ctggcggtga
    46801 gagttttatt gcagcacttt ctttagcatt agctcttgcc gatgtgatcc agacacaagc
    46861 tggtggtgta gcaatcgatg cattgtttat tgatgaagga tttgggtcat tagatgaaga
    46921 agctttggaa atggcaatgg aagctttaga aacgatcgaa agtgaaggaa gaatgatcgg
    46981 catcattagt catgtaagag aattgaaaga acgagtatta caacaaataa gaattgatac
    47041 agaaggtagc ggacaaagca aaatacgcta tcaacttgga taaaaaggga gattggtaac
    47101 tatgaaatgg agtattccag aaaaagtcat tgcacgtgga cgtcaatact tgcaagaagg
    47161 ccgagtgtta tcggttgtcc ctgaccaaga agaaaacgtg tggcatgcag aagtcttggg
    47221 cagtgaattg tatatggttg atttggatgg tacagcaaaa gagatagatt actgccaatg
    47281 tccctattgg gaggaacata aatattgtaa gcatacagtt gctgtggaac tttatttacg
    47341 gtcgaaaaat ctatctcgta tcatgaaaaa agaccagcca atgctagaac cggtaaaagc
    47401 aacaagtgag ggagaaatca ttgcaaaagg tttttctcgt ctaaaaacac aaaacaatcc
    47461 tcaagcggtg caacctttga ttattgaata ccaagtagaa acgattgaga ctaatcagta
    47521 tcacccagag ctttctattt tggcacttta cttgcgtatc ggttcattgg catcaccgaa
    47581 aaaaacatat atcgtaaaaa acatctatga ttttttacaa gcttacacag aaaaagaaac
    47641 ttacactgtt aataaacagt accagtttcg tctagataaa cgggcgtttc aaaaagaaga
    47701 tcaaaaaata ctcacgcatc ttgcaggaag tgctcagacg catcaattat taggtacgaa
    47761 cggcttacaa gtcaaaggaa aactagataa aagatatctg cttctcccag tagaacaagc
    47821 ccgcttcctg ttagagcaga tgaatcagac tacacgtttg catctgcaga taaatgaaca
    47881 aaacgatcaa ggaatacaat tttccgatca aaacaaacct ttatcattca aagtggaaaa
    47941 atttggagaa gattatttgc tgcaagcatt gaatgatttt gattttttct ttatgcatta
    48001 tcagtgggga gttatccaag ggacatttta ttctctgacg aaatatcaac aaacggttta
    48061 tcaaacttgg aaacaattga ttcgccgatt ggagaaacca gaagtagtgt tcaagaaaaa
    48121 agaattaccg tctttgttta aagagattat ccctctgctg tcagagatcg gcgacgtagc
    48181 tattgcccct gcagtagaag aggaagtagc agatatcccc gtcgaattta ttttcttttt
    48241 ccgaaaagca aaaggaaaaa tccaagctcg agtcgatttc gtctatggcg aagtcatcta
    48301 ttcgactgac ccgaagcatg aggtctcgac agaacaggct tttcaggtgt tacgtgacac
    48361 agtagaagag aaacgagtac ttgatctatt cagaatgtat cgctaccaga aaaatgaaac
    48421 tggttatgaa cgtccattgc caagcgggga agaactatat gcctttttcc gcacagaact
    48481 atctgtcttc cgtcagtttg gtgaagtcag acttggaaga aaattgcgag agttgtattt
    48541 agatgcgcat aaacatcaac cgaaaatcga agtaaccgag accggttcat ggctcgatat
    48601 tcgattcgat gtgacaggga tagaggagca agaaatcgat catgtcctgc aaagcttgct
    48661 gagaaatgat gctttttata cattagaaaa tggtcaggtt ctttcttttg attcagaaga
    48721 atttcaacaa acaagccaag tgttgcaaca attacgggaa tcgatacgta cagaagaagg
    48781 aacgatccat gtacctaaaa atcaaggatt gatcattcaa aatcaattgg aaaaaagcaa
    48841 tgctactttt agtgaatcgt tccaaacgat ggtacaagat ctcattcatc cagaaagata
    48901 ccaggcacag cttcccaaag gattgaatgc aacgatgaga gattatcaaa agcaaggttt
    48961 tcgatggttg aagatgttag gtcattatca gtttggcggt attttagccg atgaaatggg
    49021 acttgggaaa acactacaga caattgcctt tttactttcg gaaaaagaag aacggaaaag
    49081 cttctctgct ttgatcgtag cgcctgcgag cttaatttac aactggcagg cagaggtgag
    49141 aaaattcgcc ccttctttaa gtatccaagt aatcaatggg aataaaaaag aacgtgaaga
    49201 attactggct aaagacacag atatccgagt gacttcttat gccagcttaa gacaggatct
    49261 agctaattat cagtcgcaaa aaattgatta tttgattctt gatgaggcac aaatggtcaa
    49321 aaatagcagt acgaaaactg cacaagcatt gagagaatta gctgtacctc agcggtttgc
    49381 actgagtggg acaccaatcg agaataattt agaagaattg tggtcattgt ttgcaacgat
    49441 tatgccagga tttttcccga ctaaaacaaa attcagggaa cttgccgcag aagaaattgc
    49501 gcaaatgatt cgaccgttta ttttacggcg agataaacaa actgtgttga aagacttgcc
    49561 ggaaaaaaca gagatgaatc tatattctgc acttacagaa gaacaaaaaa ctgtttattt
    49621 agcttacttg cgtcaaatgc gtgaagaaat cacatcaatg gattctgaag cattcaagaa
    49681 aaaccggatc gggatacttg ctggtctgac aagactgcga caaatttgct gcgatcctcg
    49741 tctgtttatt gaagattacc aaggcggttc cggcaagctc gaacaagtaa aagatctact
    49801 ggtagctgcc aaagagaaca aacgaagagt tttgctgttt tctcaattta cagggatgct
    49861 gactatcttg caagaagagt tagccgagct agggatctcg acattttatt taagaggcag
    49921 tacgaaacca caagatcggt taagtatggt agacgcattt aatgctggcg aaaaagatgt
    49981 ttttctgatt tccttaaagg ctgggggaac tggattgaat ctcacgggag cagatacggt
    50041 catcttgtat gacctttggt ggaatcctgc cattgaagaa caagctgctg gacgtgcaca
    50101 tcggatcgga caaaaaaatg tggtagaagt gtggcgtatg atctctgaag gaacgattga
    50161 ggaacgcatg gattcactcc agcaagaaaa acgcgaactg ttccaaaaag tgatccaagg
    50221 taatgaagaa cagttgacga aactgactga agaagacatc cgcatgattt taagtgtagg
    50281 agaaatcgag gagtaagaga ctcgaaaaag cgttcagtaa tgggtaagaa agcctttcta
    50341 aagaagcgat tgtttatttg atttaaaaaa tagcaaggag gctgggattc cattttatcc
    50401 cagcttcctt gctatcattt ttttatttct atttttaaag gttatacaaa gattgtcttc
    50461 attgtctcta acagctgatc aaaagaagaa atctcgatat cgcaaattgg agtctgttca
    50521 attttgcgtt ctcgatggtt gaaccaaagc gcttgccagt tagcactttt tgcacctaga
    50581 acatcattgt cgtaattatc gccaacgtat aaggtctctt ctggaagcat atgaaattct
    50641 ttctcagcta gctgaaaaat ctctttttct ggtttttgga aacctgttgc ttgagaaatg
    50701 agcatattgc gggaaggaat ccaattgttc aattgaagtt gcttcagttt ttttgtctga
    50761 tggtcagtcg gcccatttgt gataattcct aatgggacat tcttttcctt caagaaatcg
    50821 agggtttttt tgacctcctc atgcaagcag atattatcaa gttcttcctc gtaaattttt
    50881 tgaaataaga ggccatcttc ttccgtaata tgagggtagt ctaaatcttt tagtgattgg
    50941 ctgatccgat gtgctcgcat atattctagt gtccaatctc ctgccattac ttttgggaag
    51001 ttttcatcac tatgatggcg gaaccggata tatagtggat gcatatctgc atctgaaaca
    51061 agcggaacga ctcttttgac cgcgtttcga aatggctgct gctgatcata catcgtatca
    51121 tcaacatcaa acacaactga tttaatcaaa attttacacg cctttcggtt ttcattgatt
    51181 ttttcacaaa tccttgacta ttgtaacaga aaaaattgtc taaaaaagct gataaataaa
    51241 ggtttttctt actatatttc tatttccttg acagaataag gtttttatat ttcagttttt
    51301 ttcactaaaa atcgtgaaac acgtcaataa gtttgatgaa caaattaatc attatggtag
    51361 aagtcgattg atttatttta atgcaatctc tgtggaattt tcgaaagatt tattttttta
    51421 atacagtctc aatataacta ttcaaacata aaataacgtt atttgattta tttgtttata
    51481 gtaaatttta tttagtaatc attgctttaa aatactacgc ttatataaca gagaaaataa
    51541 gcgttataat aaagaacagt attatttaaa atgacagaaa acattactat taaagaaata
    51601 aaaaacataa aaaatcttaa gaaatcggtt tcatttagct tttgagataa aataatagta
    51661 cagttttata cgctgtttca tatgtttaga aaatttgaat tagatttgaa aactctaatt
    51721 tttaaatgat aaaaaaagcg agaatgctgg ttttactgta tattaacctt agataacttt
    51781 cattttttta aaaaaattat gaaaaaaagg tagctattta aaagttgatt atgtataata
    51841 gaaacagatg ggagattatt atgatcttct gttacaaaaa agtgattctt atcacgaaaa
    51901 ggagtggtgc acgttgttaa cattatatac ttccccaagt tgtacttcct gcagaaaggc
    51961 tcgtgcgtgg ttgcaagaac atcagattcc atttgttgaa cgtaatattt tttcagaacc
    52021 attgaacagt tctgaattaa aagcgatttt acaaatgaca gaagacggaa cagaagaaat
    52081 catctcgaca cgttccaaag tattccaaaa actgaacatg gatttagatg atctgccgtt
    52141 acaagaatta ttggaacttg tgcaaaacaa tcctggattg cttcgccgtc caatcatgat
    52201 tgatgataaa cgtctgcaag ttggttttaa tgaagatgaa attcgtcggt tcctaccgcg
    52261 cgatgtacga caattagaat tacgtcaagc acaattgatg gctggattat agaaggataa
    52321 tacgaaactt ggaaaagact attacaaaga tagtttttcc aagttttttt gtttttacaa
    52381 aaagtattct tgattagatg tataaaaagg tagtagaaga tgatgattgc acgaaaaata
    52441 gagagggaaa attagctgaa aagtcggcta tatagtattc gtctgccaaa aatgttatag
    52501 ttaaataaga tagcttgaaa ggttgaaaaa tccgctggtt ttattaaatt cgcttaacag
    52561 cgagattttt tttctttaca tttaagctga tttcgctaca atgaatctat attaaacatt
    52621 gtggcaaagt gaggtgtagc aaaatggaaa tggaacatat taatgaaaat acgatccgtg
    52681 tactaattgg tcatgaagat ttagaggaac ggggcgtttc atttttggat ttacttggca
    52741 atcacaaaga aatcgaaaat ttcttttata gtattttgga agaagttgat atcgaagagg
    52801 agttccgagg aagcgaagct gtcactttcc aagttttgcc aaaaggagac ggattagaac
    52861 tgtttatcag taaaaatctt ccggatgaag aaatttctca atcagaagat tctactgttg
    52921 attcagaaga tgtgagtgac tttcttcgtc agcagattat tggcagcgat ccggaggaag
    52981 aattatctgg aagcgcccta gaagaacagg aaagacaagc aacttttgct tttgaagatt
    53041 ttgaacaagt gatccaactt gcaacagatg tcgaactaga gtctgcttgg actgatttgt
    53101 atttattcga agagcgctat tacctagtcg ttcacttctg gatggaaaat ctcaaccaag
    53161 cggatgtaga gaatcagatt gctcgaattt tggagtttgc tgaaaagagc gatcgtacac
    53221 cagaaacatt gaatgaatat ggacaatgtc tgatggcgcg taatgctatc gagcgaacga
    53281 aattttattt tgtataaatc agcattatag taagagactg tgacaaaaat tgtgtcgcag
    53341 tctttttttc gtataaatgt gttcaaaagc ttgcatctgc ggtaacaagc ccaaccaaac
    53401 gaaaaatatt gggaactatt tttgtttagc tgttcttatt acttgatgca gaatagcttt
    53461 ttcacgccct cttttttcgt gtaaataaat tcttagacgt aaataagttc agagggagaa
    53521 aaatgctgct tgcaatgaat gaatataata agtgctgttt tgctaaacat gcagaaaaag
    53581 gaaagaaata ttattgtcgt ggctgtcgaa aaagagtgat tttgaaaaag ggaaagaaaa
    53641 aatgtgctca ttttgcccat caaaaatcag ataactgttc ggtctttagt gaaagagaat
    53701 caaaagagca cttacagtta aaggaatgct tcatggattg gctgggacag tcagtagaac
    53761 cagtattttt ggaagcctat ttgccgaggt tgcggcaaag gccggatatt ttgttggcga
    53821 atctagcaat agaaatccaa tgttctagac tgtctcacca gagatttatt gaaagaaccc
    53881 aaaattattt gaataacagt taccaagtat ggtggatact tggtaattct tttcttggac
    53941 aatcgcaatt ctcattgata gaaaaaagtt gctgctacta taatagaaaa agaggagtgc
    54001 attgctggaa agctgatctc aagaaacaaa agctttattt gtatcatcat attacagaga
    54061 ctgtatcagg gcatatttcc ttttgcagta gctgctggac tttttctagt agggacctta
    54121 aagaaatatt cactagtaac gaaattaaaa tgaaccaaat gaaaaaaata gaaaaagtga
    54181 gtgaagatgg gaagaaatgg ctgacacgcc aacttattca caagcaaaag aatacagtat
    54241 ccatacaaga acaatgctac ttgcgtcata aacatctttt gcatctatca tcgtggatct
    54301 atcaaaaaag tcggtttttc ttttatttac aagaacaggt ttttctttat cgaatgttgt
    54361 acgaagaagc cttggaaaac cagaaagttc ctgatttcaa ctcatggctt tgccaaataa
    54421 aagaacataa aagggaatgg ctttttccta tgattgatga agagatggtc tatcagcagt
    54481 tttttaacga atgcatacac ttgtcttctt tgaaaaaata gtaagtataa aaataggcta
    54541 ggaatcggtt ttctatgtaa aacaagattt agttatgcta aaattgatgg aagcagacac
    54601 atgaatgagt agaaaagtta gatgctgtga catgtggatg ttagactatg atctattttg
    54661 atagatagag ctaagggaag aaaaggagtg gatttcatgg aagtaaagca gttgccaaaa
    54721 cgagaagaat tgcctgaaaa tttaacttgg gacttgacca agatcttttc aagcgaccaa
    54781 gagtttgatg agaaatattt ggaattatca gaagagttaa aacaatctga aaaacacaaa
    54841 ggaacacttg atcaaggcgc ttctcaattt ttaaatgcga ttgaattcgt attgagggtt
    54901 tatcgccaaa ctgaagtcat ttatgtatat gcgcacctta aaaacgatca agacactgga
    54961 aatacagatt accaagcgct ttatgcaaga gcaagcagtc tgttttcgaa agttagtgaa
    55021 gccgtttcct ggtttgaacc agaaatattg caattgtcag atgaccagat ttggcaatat
    55081 ttcaaagaag aaccaaaatt ggaagtctat cgccattata tccagcaaat agtagataat
    55141 cgagcccatg tcttatctgc tgagcaggaa tctcttcttg ctggagcagg tgaaatcttt
    55201 gatgcttcaa gtgatacatt tgctgttttg aataatgcag atctagtttt tccaacgatt
    55261 gaaggagaaa atggtgaaat agtccaatta tctcatggcg tgtatggtca gttgctagaa
    55321 agcacggatc gaagggtgcg cgaagcagca tttaaggggt tgtacagtgt ttacgaacaa
    55381 tttagaaata catttgcttc tactttaggc acacatataa aaggacataa ttttaaagcg
    55441 aaagtccgta attacagctc tgccagagaa gcgtctttga gcaataatca tattcctgaa
    55501 agtgtatacg atactttggt agacgtggta aacaagcatt tgcctttgtt acatcgatac
    55561 atggaattac ggaaacgttt attagaagtg gaaaaactgc acatgtatga tctttataca
    55621 ccggtcttag gggaagctcc aattaccttt acgtacgaag aagcaaaaga aaaagcttta
    55681 gaagcactga aaccaatggg tgaagaatac atggccatcg tagaaaaagc attctctgaa
    55741 cgttggatcg atgttgtcga aaataaaggg aaacgaagcg gtgcttattc ttcgggaagc
    55801 tatgacacaa atccatatat tttattgaat tggcatgata cgctggatca gctatttacg
    55861 cttgtccacg aaatgggaca tagtgttcat agttatttca ctcgttcgaa ccagccttat
    55921 gtgtacggcg actactccat ctttttagca gaaattgctt cgacaacgaa tgaaaatatc
    55981 ctaacggagt atttattgga aacagaaaaa gaccctcgtg tacgggctta tgtactcaac
    56041 cattacttgg atgggtttaa aggaacagtt ttccgccaga cacaattcgc tgaattcgag
    56101 catttcatgc atacggaaga tgaaaaaggt gtgccattga ctagtgaata cctaagtgat
    56161 agttatggta aattgaatgc aaaatattat ggtccagcag tcgaagaaga cccagaaatt
    56221 aaattcgaat ggtcacggat accgcatttt tattataatt actatgtttt ccaatactcg
    56281 actggctttt ctgctgcttc agcactggcg aagaaaatac taaaccagga accagaggca
    56341 ctagagaact acttggctta cctaaaagca ggtaacagcg attatcctgt ggaagtaatg
    56401 aaaaaagcag gagttgatat gacacaggct gcatatattg aagatgcaat gtcgatgttt
    56461 gaacaacgtc taaatgaatt agaagaattg atcgatcgtt tataaggctt tctatttttg
    56521 attttctcga atagaaacga aaaaaatacg tcggctctct tgtaagggag tcgacgtatt
    56581 ttttta
//
LOCUS       DACDYM010000007        55273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDYM010000007 DACDYM010000000
VERSION     DACDYM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.007"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(136..2289)
                     /locus_tag="IWM59_000460"
     CDS             complement(136..2289)
                     /locus_tag="IWM59_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9978518.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
     gene            complement(2583..3431)
                     /locus_tag="IWM59_000461"
     CDS             complement(2583..3431)
                     /locus_tag="IWM59_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9978519.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IWM59_000462"
     CDS             complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IWM59_000462"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9978520.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5932..6111)
                     /locus_tag="IWM59_000463"
     CDS             complement(5932..6111)
                     /locus_tag="IWM59_000463"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9978521.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IWM59_000464"
     CDS             complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IWM59_000464"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9978522.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8259..10538
                     /locus_tag="IWM59_000465"
     CDS             8259..10538
                     /locus_tag="IWM59_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9978523.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10631..11278)
                     /locus_tag="IWM59_000466"
     CDS             complement(10631..11278)
                     /locus_tag="IWM59_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978524.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11457..12176)
                     /locus_tag="IWM59_000467"
     CDS             complement(11457..12176)
                     /locus_tag="IWM59_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9978525.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12307..13458)
                     /locus_tag="IWM59_000468"
     CDS             complement(12307..13458)
                     /locus_tag="IWM59_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9978526.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13675..14415)
                     /locus_tag="IWM59_000469"
     CDS             complement(13675..14415)
                     /locus_tag="IWM59_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9978527.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14419..14742)
                     /gene="rsfS"
                     /locus_tag="IWM59_000470"
     CDS             complement(14419..14742)
                     /gene="rsfS"
                     /locus_tag="IWM59_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9978528.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14799..15392)
                     /locus_tag="IWM59_000471"
     CDS             complement(14799..15392)
                     /locus_tag="IWM59_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9978529.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15376..16023)
                     /locus_tag="IWM59_000472"
     CDS             complement(15376..16023)
                     /locus_tag="IWM59_000472"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9978530.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16036..16347)
                     /gene="yhbY"
                     /locus_tag="IWM59_000473"
     CDS             complement(16036..16347)
                     /gene="yhbY"
                     /locus_tag="IWM59_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9978531.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16363..17472)
                     /gene="yqeH"
                     /locus_tag="IWM59_000474"
     CDS             complement(16363..17472)
                     /gene="yqeH"
                     /locus_tag="IWM59_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9978532.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17469..17996)
                     /locus_tag="IWM59_000475"
     CDS             complement(17469..17996)
                     /locus_tag="IWM59_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9978533.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18176..18961)
                     /locus_tag="IWM59_000476"
     CDS             complement(18176..18961)
                     /locus_tag="IWM59_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9978534.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18954..19823)
                     /locus_tag="IWM59_000477"
     CDS             complement(18954..19823)
                     /locus_tag="IWM59_000477"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9978535.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19824..21203)
                     /locus_tag="IWM59_000478"
     CDS             complement(19824..21203)
                     /locus_tag="IWM59_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9978536.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAKQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21211..21630)
                     /gene="fabZ"
                     /locus_tag="IWM59_000479"
     CDS             complement(21211..21630)
                     /gene="fabZ"
                     /locus_tag="IWM59_000479"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9978537.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21655..22131)
                     /gene="accB"
                     /locus_tag="IWM59_000480"
     CDS             complement(21655..22131)
                     /gene="accB"
                     /locus_tag="IWM59_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9978538.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22134..23369)
                     /gene="fabF"
                     /locus_tag="IWM59_000481"
     CDS             complement(22134..23369)
                     /gene="fabF"
                     /locus_tag="IWM59_000481"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9978539.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23388..24125)
                     /gene="fabG"
                     /locus_tag="IWM59_000482"
     CDS             complement(23388..24125)
                     /gene="fabG"
                     /locus_tag="IWM59_000482"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9978540.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24137..25054)
                     /gene="fabD"
                     /locus_tag="IWM59_000483"
     CDS             complement(24137..25054)
                     /gene="fabD"
                     /locus_tag="IWM59_000483"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9978541.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25161..25385)
                     /locus_tag="IWM59_000484"
     CDS             complement(25161..25385)
                     /locus_tag="IWM59_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9978542.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25438..26400)
                     /locus_tag="IWM59_000485"
     CDS             complement(25438..26400)
                     /locus_tag="IWM59_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9978543.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26410..26850)
                     /locus_tag="IWM59_000486"
     CDS             complement(26410..26850)
                     /locus_tag="IWM59_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9978544.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27156..28121
                     /locus_tag="IWM59_000487"
     CDS             27156..28121
                     /locus_tag="IWM59_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9978545.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28178..28408)
                     /locus_tag="IWM59_000488"
     CDS             complement(28178..28408)
                     /locus_tag="IWM59_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9978546.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28494..29381)
                     /locus_tag="IWM59_000489"
     CDS             complement(28494..29381)
                     /locus_tag="IWM59_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9978547.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29470..30693)
                     /locus_tag="IWM59_000490"
     CDS             complement(29470..30693)
                     /locus_tag="IWM59_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9978548.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30710..31789)
                     /locus_tag="IWM59_000491"
     CDS             complement(30710..31789)
                     /locus_tag="IWM59_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9978549.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31920..33647)
                     /gene="ptsP"
                     /locus_tag="IWM59_000492"
     CDS             complement(31920..33647)
                     /gene="ptsP"
                     /locus_tag="IWM59_000492"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9978550.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(33647..33913)
                     /locus_tag="IWM59_000493"
     CDS             complement(33647..33913)
                     /locus_tag="IWM59_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9978551.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34392..36626
                     /locus_tag="IWM59_000494"
     CDS             34392..36626
                     /locus_tag="IWM59_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9978552.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36774..37094
                     /locus_tag="IWM59_000495"
     CDS             36774..37094
                     /locus_tag="IWM59_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9978553.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37299..37523)
                     /locus_tag="IWM59_000496"
     CDS             complement(37299..37523)
                     /locus_tag="IWM59_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP9978554.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37721..39301)
                     /locus_tag="IWM59_000497"
     CDS             complement(37721..39301)
                     /locus_tag="IWM59_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9978555.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39702..41078)
                     /locus_tag="IWM59_000498"
     CDS             complement(39702..41078)
                     /locus_tag="IWM59_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9978556.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41206..42324)
                     /locus_tag="IWM59_000499"
     CDS             complement(41206..42324)
                     /locus_tag="IWM59_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9978557.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42466..42900
                     /locus_tag="IWM59_000500"
     CDS             42466..42900
                     /locus_tag="IWM59_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9978558.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(42978..43511)
                     /locus_tag="IWM59_000501"
     CDS             complement(42978..43511)
                     /locus_tag="IWM59_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9978559.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(43605..44783)
                     /gene="mutY"
                     /locus_tag="IWM59_000502"
     CDS             complement(43605..44783)
                     /gene="mutY"
                     /locus_tag="IWM59_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9978560.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(44776..45573)
                     /gene="recX"
                     /locus_tag="IWM59_000503"
     CDS             complement(44776..45573)
                     /gene="recX"
                     /locus_tag="IWM59_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9978561.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            45680..47062
                     /gene="rlmD"
                     /locus_tag="IWM59_000504"
     CDS             45680..47062
                     /gene="rlmD"
                     /locus_tag="IWM59_000504"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9978562.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(47236..47814)
                     /locus_tag="IWM59_000505"
     CDS             complement(47236..47814)
                     /locus_tag="IWM59_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9978563.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            48136..48606
                     /locus_tag="IWM59_000506"
     CDS             48136..48606
                     /locus_tag="IWM59_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9978564.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(48714..49940)
                     /locus_tag="IWM59_000507"
     CDS             complement(48714..49940)
                     /locus_tag="IWM59_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9978565.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(50694..50894)
                     /locus_tag="IWM59_000508"
     CDS             complement(50694..50894)
                     /locus_tag="IWM59_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9978566.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(51028..51636)
                     /locus_tag="IWM59_000509"
     CDS             complement(51028..51636)
                     /locus_tag="IWM59_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9978567.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(51721..52191)
                     /locus_tag="IWM59_000510"
     CDS             complement(51721..52191)
                     /locus_tag="IWM59_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9978568.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(52239..53153)
                     /locus_tag="IWM59_000511"
     CDS             complement(52239..53153)
                     /locus_tag="IWM59_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9978569.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            53299..54102
                     /locus_tag="IWM59_000512"
     CDS             53299..54102
                     /locus_tag="IWM59_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9978570.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            54280..55227
                     /locus_tag="IWM59_000513"
     CDS             54280..55227
                     /locus_tag="IWM59_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9978571.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT        15906 a        11668 c         9444 g        18255 t
ORIGIN      
        1 cttttttgcc gagtaaacgg tgttcaaaag ccagtttctc ggtatgaagt caaatttaag
       61 caaaaatgtg agaagctatt ttcacaaaat ttttcttcat actcgaaact aagcggcttt
      121 ttcacgacct cttttttatt ttgccatgaa catatcataa tacgcgtcca ttatttcttt
      181 agcaatcgtc aagttcatgt ggtcttgttc gtctaataag ttcggcaaga caacactgat
      241 ggcaatttcc ggattgtctg ttggtccgta tgccacgata ttactattga ctgtcgtaat
      301 atctggatga ccttccgcaa ccgtttctgc agtccctgtt tttgcggaaa ggtccatttt
      361 cgcactggct aatggtttgg ctgctgtgta aggatcggta ccatggacaa cttggtggaa
      421 tccttcacgg aggatcgcca tattttctgc agaaatattg accgtatttt ctactgttgt
      481 gccaatttct ttcttcactt cacccaagtt tccattttcg tcattgccat agatcgcttt
      541 gactaggtgc ggctgtactc gtttcccgcc attagcgact gttgaagcat attgagctaa
      601 ttggattggt gtatatgtgt caaactgtcc aaatgccaaa tcggtaaagt tacctggagt
      661 ataccattct ttgccattat ccgcattgaa cttcttcata taatcaacag aacgtgagat
      721 cccaggagat tcattcggca aatcgatccc agtcgttgta ccaagaccaa actctttgaa
      781 agagtctctc aattgctgat aagctttagc ttcttcatct aaactaggta gtcccattcc
      841 aggtgtatag tctagtccta acatctttaa cgccactttg atcatatacg tattagaaga
      901 caattccaat gctttgactg catcaagagc aacttgtccg cttcgattaa agacagagct
      961 tttttctgaa gcaccttgca gacgaatcgg ctcatcaatc aaaacttgat ttccgctgat
     1021 cgcgttactt gcccagcctg ctgtcaaagt accagccttg actacagacc ctggagcaaa
     1081 ggcgctagta atcgtgccta aagcattctc ggtgatttct ttggaacctt gttcatgact
     1141 gaaaccagtc attgctagca cttctcctgt ctgaggattc atagccactg cataagcacc
     1201 aggcgaatac tgtgctttgc cgttgttgat caatgtttga taattccttt tcaaaatttc
     1261 ttctactttt gattggaatt ctgcattcat agataacatt aaattagaac ctttttcccc
     1321 agagaagatt tctttttgat tcgaaacatt tccttcatta tcaagggaaa cttcatattg
     1381 ggttttggtt ccttgcagga catcttcata ttgtttttcc aaataacttt gtcctacacg
     1441 gtcattccga gaataacctt ttgctaggta tttctctgct tcatctgctg gaagtccttg
     1501 cttttcagtc gtcacactgc caagaatact tttcagtgaa cttgctgcat tatattctcg
     1561 tgtccagtct gtgccagtcg aaactcctgg aagttcagaa gcgcgttctg ccacaacagc
     1621 taactcttga tcagtaacac cagagttttt gatatagaca gtgtttaatt catatgcact
     1681 attcatccgt ttaaagatgg tagcaatttt taattgctcc tcgtttaaat taatctcatc
     1741 agctgtgact ttattgacca tcgcactata ttgttcgctg ttccctaatt gcttttcttt
     1801 tgccgaaagt ctttttgtgg cttctttcaa atgtttggga tcagccagcc aaaaatcttt
     1861 tttatctcta tctgtcagat tttcatctac cggaacatcg atcagcgagt tcagcttcgt
     1921 tgcgatttca agcaaatctt tagccgtaac ttgtgttcct ctcgtatatg tgatggcctg
     1981 attcgcttta ttcgtaacta aagcttttcc tgtggagtcg tagatcattc cgcgaggagt
     2041 actttcttgg accgtaatcg tagaagtaga cttcactcgc gcctccatct cttcgccgtt
     2101 cacgatctgc aaataaccca atctagcaat caacgcaaca aataacgtaa atataatgaa
     2161 aaaaagaaaa tttaagcgaa aaggaacatg ggaccggcga aacgttttgt tcttaggttc
     2221 ttgtgcacct tttcttattt tttctaacca agaagtattt aatatttttt tcataaaatc
     2281 tttcatcatt tcctatcctt ccgcctattt acattcatgc ttcattgtac ccaaaaactt
     2341 cttaataaaa aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa
     2401 tcaacttttt gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa
     2461 aaaataaact tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca
     2521 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
     2581 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
     2641 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
     2701 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
     2761 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
     2821 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
     2881 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
     2941 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
     3001 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
     3061 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
     3121 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
     3181 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
     3241 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
     3301 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
     3361 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
     3421 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
     3481 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     3541 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     3601 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     3661 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     3721 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     3781 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     3841 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     3901 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     3961 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     4021 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     4081 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     4141 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     4201 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     4261 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     4321 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     4381 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     4441 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     4501 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     4561 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     4621 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     4681 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     4741 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     4801 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     4861 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     4921 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     4981 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     5041 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     5101 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     5161 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     5221 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     5281 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     5341 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     5401 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     5461 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     5521 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     5581 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     5641 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     5701 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     5761 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     5821 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     5881 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     5941 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     6001 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     6061 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     6121 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     6181 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     6241 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     6301 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     6361 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     6421 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     6481 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     6541 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     6601 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     6661 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     6721 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     6781 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     6841 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     6901 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     6961 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     7021 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     7081 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     7141 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     7201 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     7261 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     7321 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     7381 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     7441 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     7501 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     7561 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     7621 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     7681 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     7741 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     7801 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     7861 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     7921 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     7981 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     8041 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     8101 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     8161 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     8221 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     8281 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     8341 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     8401 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     8461 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     8521 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     8581 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     8641 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     8701 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     8761 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     8821 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     8881 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     8941 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     9001 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     9061 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     9121 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     9181 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     9241 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     9301 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     9361 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     9421 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     9481 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     9541 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     9601 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     9661 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     9721 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     9781 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     9841 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     9901 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     9961 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
    10021 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
    10081 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
    10141 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
    10201 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
    10261 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
    10321 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
    10381 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
    10441 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
    10501 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat
    10561 taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat
    10621 ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa
    10681 gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc
    10741 tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt
    10801 ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac
    10861 gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc
    10921 tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag
    10981 cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca
    11041 gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta
    11101 aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga
    11161 atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta
    11221 ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat
    11281 tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc
    11341 ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta
    11401 gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag
    11461 tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca
    11521 atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc
    11581 atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata
    11641 ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg
    11701 cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca
    11761 aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc
    11821 ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc
    11881 aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag
    11941 tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt
    12001 acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca
    12061 ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta
    12121 ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt
    12181 tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt
    12241 gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc
    12301 aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa
    12361 ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat
    12421 tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa
    12481 tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt
    12541 cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac
    12601 ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc
    12661 tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg
    12721 ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc
    12781 ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat
    12841 ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt
    12901 acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact
    12961 taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg
    13021 aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc
    13081 ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga
    13141 agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac
    13201 tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac
    13261 taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg
    13321 ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat
    13381 ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat
    13441 aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga
    13501 caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa
    13561 cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat
    13621 ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc
    13681 tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc
    13741 agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa
    13801 tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg
    13861 ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact
    13921 atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc
    13981 atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg
    14041 atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc
    14101 agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc
    14161 cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc
    14221 ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc
    14281 tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga
    14341 aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa
    14401 tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga
    14461 ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac
    14521 atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc
    14581 atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc
    14641 gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa
    14701 agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc
    14761 tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa
    14821 ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat
    14881 gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca
    14941 tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg
    15001 tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct
    15061 gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt
    15121 gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac
    15181 tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca
    15241 ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc
    15301 ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa
    15361 gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa tataatcaat
    15421 tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt
    15481 cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt
    15541 tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt
    15601 ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc
    15661 cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc
    15721 taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt
    15781 gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg
    15841 catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg
    15901 tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa
    15961 gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt
    16021 catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt
    16081 tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat
    16141 ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga
    16201 tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt
    16261 gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa
    16321 acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg
    16381 tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt
    16441 gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg
    16501 aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac
    16561 atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt
    16621 tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc
    16681 gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa
    16741 cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat
    16801 aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa
    16861 ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat
    16921 gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg
    16981 tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc
    17041 actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca
    17101 ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa
    17161 aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc
    17221 aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg
    17281 caaaaagtcg tcatccgtca atgagacatc ttggatctca ttataatggc gtaatctgaa
    17341 acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt
    17401 gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa
    17461 ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag
    17521 gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata
    17581 atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata
    17641 atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg
    17701 ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca
    17761 atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca
    17821 tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca
    17881 atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca
    17941 ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg
    18001 tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact
    18061 gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt
    18121 acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat
    18181 attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt
    18241 ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact
    18301 gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg
    18361 gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa
    18421 tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt
    18481 catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga
    18541 tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc
    18601 cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg
    18661 gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat
    18721 ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga
    18781 tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg
    18841 caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca
    18901 agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca
    18961 cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat
    19021 tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg
    19081 aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg
    19141 atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact
    19201 cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt
    19261 ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt
    19321 gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat
    19381 aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc
    19441 atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca
    19501 tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc
    19561 ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa
    19621 gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct
    19681 tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta
    19741 tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat
    19801 ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc
    19861 ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga
    19921 ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt
    19981 aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt
    20041 gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact
    20101 cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca
    20161 aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact
    20221 acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact
    20281 aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt
    20341 tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca
    20401 taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt
    20461 gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa
    20521 tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata
    20581 tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct
    20641 gcttcttgct tagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga
    20701 atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct
    20761 gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg
    20821 acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt
    20881 gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta
    20941 ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg
    21001 ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt
    21061 gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg
    21121 gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta
    21181 gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc
    21241 ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac
    21301 tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt
    21361 tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa
    21421 tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc
    21481 ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat
    21541 ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg
    21601 aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt
    21661 ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat
    21721 ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat
    21781 aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc
    21841 gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa
    21901 tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac
    21961 attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact
    22021 tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag
    22081 cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc
    22141 tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt
    22201 gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga
    22261 tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt
    22321 gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg
    22381 ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc
    22441 tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt
    22501 acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta
    22561 ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt
    22621 ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca
    22681 cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt
    22741 gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct
    22801 tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc
    22861 ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca
    22921 cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt
    22981 ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga
    23041 agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta
    23101 tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata
    23161 tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca
    23221 cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc
    23281 gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact
    23341 ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg
    23401 accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa
    23461 actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc
    23521 gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc
    23581 gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat
    23641 cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca
    23701 gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg
    23761 tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct
    23821 gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg
    23881 atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag
    23941 atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct
    24001 tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag
    24061 gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca
    24121 tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa
    24181 cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg
    24241 ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga
    24301 aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta
    24361 tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta
    24421 ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga
    24481 catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta
    24541 ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata
    24601 cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca
    24661 tcactgcaac cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt
    24721 ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat
    24781 attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc
    24841 gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac
    24901 gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat
    24961 caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg
    25021 caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat
    25081 atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc
    25141 ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga
    25201 accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg
    25261 atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat
    25321 ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat
    25381 accataataa tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta
    25441 caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt
    25501 ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata agatagggat
    25561 gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat
    25621 ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata
    25681 atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa
    25741 atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata
    25801 gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc
    25861 tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc
    25921 tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt
    25981 ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct
    26041 taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc
    26101 tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt
    26161 tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc
    26221 cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat
    26281 acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt
    26341 agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat
    26401 taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa
    26461 ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa
    26521 atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg
    26581 gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc
    26641 agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt
    26701 cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg
    26761 agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata
    26821 atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat
    26881 caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc
    26941 atttttttac tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca
    27001 gcatttataa tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt
    27061 attttctgtg aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt
    27121 atagtctttt tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc
    27181 tccccatgct gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg
    27241 gtaaagcaaa acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt
    27301 caaaaaaagt attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca
    27361 cggaattaga tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata
    27421 accgacaaat gaattatttc agtaattata attatgtaca tattaccaat aagggatttg
    27481 tattaacaga tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc
    27541 cgtctatttt gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt
    27601 tgttagcttt gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa
    27661 aggaactcca agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa
    27721 gacaacaaca tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata
    27781 tcgaattagt tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac
    27841 catcgctcac cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg
    27901 tttgttatgg tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct
    27961 attatgaaaa gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg
    28021 atcctgtaca aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg
    28081 aactgttaaa caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt
    28141 aggcatcttc aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt
    28201 ccatcgctac gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta
    28261 attcaaatgt atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt
    28321 caaaacggcg aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta
    28381 tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat
    28441 tgtatcaaaa gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat
    28501 tttttttctg gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca
    28561 ttctcaaaac ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc
    28621 aatacaagat ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt
    28681 ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa
    28741 gaccagttag ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg
    28801 tttttttgtg catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc
    28861 tgattagcca tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta
    28921 ccaaattctt tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg
    28981 atcgtcagtg taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca
    29041 tctgcgcctt tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta
    29101 gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag
    29161 tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct
    29221 gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt
    29281 gcttttgtcc gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca
    29341 gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa
    29401 tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat
    29461 gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc
    29521 ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat
    29581 catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg
    29641 atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt
    29701 ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc
    29761 cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga
    29821 agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc
    29881 cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa
    29941 ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat
    30001 gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa
    30061 agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc
    30121 ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg
    30181 aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac
    30241 tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat
    30301 cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat
    30361 cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt
    30421 atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc
    30481 tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac
    30541 atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc
    30601 atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg
    30661 aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga
    30721 cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat
    30781 ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt
    30841 gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt
    30901 ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac actggaatat
    30961 ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct
    31021 cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc
    31081 cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga
    31141 tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca
    31201 tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac
    31261 aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca
    31321 ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac
    31381 ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg
    31441 ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga
    31501 taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt
    31561 ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga
    31621 tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt
    31681 tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat
    31741 ataataatac cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac
    31801 tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa
    31861 aaagaacaaa aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt
    31921 tattttacat attcatgaac aagttcaacg acttcttcca tcgtatcgca ttctttcaat
    31981 gcacgatcag caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt
    32041 ttaagaatag atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga
    32101 acagctgttt gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt
    32161 gctgcatcaa ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag
    32221 taagaaacgc gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta
    32281 cctacagaga agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga
    32341 atctcgatca tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat
    32401 ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc
    32461 atagggaaca tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt
    32521 gtacggaaca tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag
    32581 aatggattca tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc
    32641 attgtacgaa caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca
    32701 tattggtcat cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac
    32761 agaccaactg cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg
    32821 tttgcagcta attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt
    32881 tcccattctg ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt
    32941 tcatctgtag gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct
    33001 tctttgactt tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt
    33061 gccatgatag cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa
    33121 ttgcgatcta attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct
    33181 tcgttgatca ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta
    33241 gcaacgtcac gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg
    33301 aacataccga tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg
    33361 tctttaatat tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg
    33421 gcatcaaata cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc
    33481 tgcaattctt cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta
    33541 tcttctactg ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca
    33601 acggctacac cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc
    33661 cttctttctt cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg
    33721 cagtaatagt aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt
    33781 tcaagtttac tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg
    33841 tttgtactaa tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt
    33901 ctttcttttc catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt
    33961 cttttttagt aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca
    34021 ttttttttcg ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt
    34081 ccggattctt tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc
    34141 ttgtcgcttt ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat
    34201 atgaaacgaa ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga
    34261 atggtcaata gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat
    34321 tcttttacct catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac
    34381 gtgaatgatt catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg
    34441 ccactgtcaa tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca
    34501 aaaatcaaca aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca
    34561 gtttggacga tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc
    34621 aaacaccacc aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag
    34681 gcaatacaga aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag
    34741 gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa
    34801 atcgccgaac caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag
    34861 ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca
    34921 aagaagtcat tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat
    34981 ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat
    35041 tcatagatga agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg
    35101 ctggtaatat tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta
    35161 ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg
    35221 tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac
    35281 gttatgaaga ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt
    35341 tatccaatcg ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg
    35401 aaacaggttc taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa
    35461 aactgcaaga agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag
    35521 cagcttatta tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg
    35581 aagaggaaac acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag
    35641 gtattccagt gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg
    35701 acttgaaagc tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc
    35761 gccgaaaccg tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg
    35821 gaccaactgg tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt
    35881 cacaagattc aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta
    35941 aattgattgg ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa
    36001 aagtacggag aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag
    36061 atgttttgca tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa
    36121 gaacggtcag ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag
    36181 tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc
    36241 aattaaacaa ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca
    36301 gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc
    36361 aaatgctggc agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag
    36421 ttgacttagg ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac
    36481 aaatcgaaga tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag
    36541 caaaactgga taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag
    36601 aatcagccga agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg
    36661 agaaatgccg tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag
    36721 aaattataat aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat
    36781 tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg
    36841 cccatctagt caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg
    36901 aacataatga aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag
    36961 atgctaaaaa ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca
    37021 aagtaatcga attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta
    37081 cattagccgt gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaag
    37141 aacacttttg tctcacgctc taaacacgta taaacggcga aagcagaagc aactccttcg
    37201 gaaataagct gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc agcttatttc
    37261 ttggagttaa acgcttctgt ctcagcctct ttttctttct agctttttga tttttctctt
    37321 ttcttttgta cttttctctt ttcattttca gttctacgcg tgtctttatt tttaactttt
    37381 tcctctttat ggatgattga tcgtactttt tgtgcaattt gctcgttttt gtcccagtct
    37441 tttacatcta ataatcgaca taatcgtaac atctcttctt ttttctgttt ttttctttca
    37501 cgtctttgcc attctgtttc catgtgtcac actctccttt ctttaatcat atgtacgcaa
    37561 aataattttt atattttttt gattagacat ttctatgatt ttaatatagt aaaaagagcc
    37621 gtcacggaaa atccgtcacg gctctaaaca aaatatgaga ggctgttttt gtttggctat
    37681 tcttattact tgatgcaggg cagcttgtgt caagacttct ttacatcaaa ctttttaatt
    37741 ttacattggg atatttatcc gcaaaccagc gttctgcaaa ttggttctcg aataagaaca
    37801 acggttggtc gaaacggtca cgagccaaga tattgcgact tgaactcatc ttctcatcca
    37861 aatcttctgg atcgatccag cgagcgattt tgtgtcccat tggagacatg attacttcgg
    37921 cgttatactc atttaacata cgatattgga atacttcgaa ttgtagctga ccaacagctc
    37981 caatgatata ttcttccgtt agatatgttt tataaagctg aatcgctcct tcttgaacta
    38041 attgatagat tcctttgtgg aaggattttt gtttcatgac attttttgca gtcactttca
    38101 taaacagctc tggagtaaat gaagggagtt cttcataagc aacttttagt tttccttcga
    38161 acaatgtatc cccaatctga taattgcctg tatcatatac accgatgata tcgcctgcta
    38221 ctgctttttc gacgttttct ctcgaatctg ccataaattg tgtgacattg cttaatttca
    38281 gttttttatt tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt ccagaacaga
    38341 ttcggacaaa agcgattcgg tcacgatggg ctggattcat atttgcttgg atcttgaata
    38401 cgaaacctga aaattcttcc tcataaggac ttaccatctg atcatcttgt gttttgtgtc
    38461 cataaggagc tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca ccgaagttag
    38521 tcaatgcaga accaaaaaat actggtgtct gatcgccacg agcaattttt tcttgattaa
    38581 atgcatctcc agcctctttg attagttcta cttcttctaa gacttgttca tataaggaat
    38641 tttcagttag aggatgattt tcgggaagca gtccatcttc atccaattct actaatcgtt
    38701 catcctgata attttccgga cgataaaact ctacacgatt atggtagata tcataaagac
    38761 cttctagccc tttacccata ccgattggcc aattcatcgg ataagattcg atatctaata
    38821 gctcttctaa ttcttctaac aattccaatg gttcacgacc atcacgatct agtttgttga
    38881 taaaagtaaa tatcgggatg ccacgttttt ttacaacttg gaataatttc tttgtctgtg
    38941 cttcgatccc ttttgcacta tcgattacca tgaccgcact atccacggcc atcaaagtac
    39001 gataagtatc ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt actcgtttcc
    39061 cttgataatc aaactgcata acagaactag taacagaaat cccacgttgt ttctcgattt
    39121 ccatccagtc tgatttggca aaattacctg tttttttgcc ttttacggtc ccggcttgac
    39181 ggatagcccc accaaacaat agtaattgtt ctgtaatcgt tgttttccca gcatccggat
    39241 gggaaatgat cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt ggattattca
    39301 taacctaact ccatttcttc cattcaatta tttatatttt ctctaggcac agattgtccg
    39361 atctcttaaa gaaagacaat ttcatatagg gctgtgacaa aagccgtgta gcagtctctt
    39421 tttccgagta aacggcgttc tagagacagc ctatcagcaa taagcccacc caagccaaaa
    39481 atacgaaaag ctgtttttgc ttggctgctc ttattactta atgcaggata gctttttcac
    39541 aacctttgtc aactactaca actgaatcag tatagcatgg attcagcgaa tgaaaaagcc
    39601 accaaaccat aagagtcaaa tatttttttg tcagctgact aaaagaaaag acgtgttgct
    39661 tgtttattca agcaacacgt ctttcataac caagtttcca attatcttct tggctcgtca
    39721 tcttcgaatt cttttctgaa gtatcttcgt tcttcttcag gttccgcttc tgtttcttcc
    39781 ggatcatgaa aaatgacacg gattttaagg attcgagaac cttccatttc ttcagaaacc
    39841 agcgtaatat tatcgacatc aaacgataac ttttcgcctt catccggaat catgcctaat
    39901 gctgtaatca aataaccagc catcgtatct acatcactca tatgcagatc actgtcaaaa
    39961 gcttcattga actcatcaat cagcatacgt ccttggatga tatattcgtg ttcatcgatt
    40021 ttttcataaa gattctcaac ttcatctgat tcatcatcga tttctccgac gatttcttct
    40081 aaaaggtctt ctagtgttgc taacccaacg acgccgccat attcatctaa taaaatagcc
    40141 atctgattct gcgtcttttt catttcatat aacagatcat caataaagac cgtttcagga
    40201 acgaataaag gctcctgcat gattttcttg atatctaaat tatcaaagcc gaatttatgg
    40261 gcagctttta gtaaattctt tgtatgaagg attccgacta ctttatcttt gtcttcgtta
    40321 taaacaggga ttcttgaata gttttcggat aaaacttcat tcacattttc ttccacagtg
    40381 tcgttgatat caatcataaa tgcatccgtt cgtgggacca tgacttctcg ggcaactttc
    40441 gtatcaagag aaaaaacccc ttggagcatt tctaattctt cattattcaa tacgccttcg
    40501 ttttcaagca tataacgcat ttcatctctg gtcatttttg aatcttcatc atcaaaagtc
    40561 atcggcgtga tacgcgccaa taaacttgtg gaagcagaaa gcagccagac gaagggctta
    40621 gctactacac caatcaagcg tatcagccct gaagtgaact ttgcgacttc ttctgattta
    40681 tttaaagcaa tacgtttagg gtacaattct ccaaaaacaa tcgatacata ggtcaaaata
    40741 gccagcacaa tgatatttgc aatatttttt gccgctgtgc cgccacctaa taccggagct
    40801 aaccgttccg ataaagtatt agctagtgaa gcaccagaca aaatgttgac caaagtgatt
    40861 cctacttgga ttgtcgatag gaaattattt ggattctgca atatttttaa aagcttttga
    40921 gatttcacat ctccttcatc agctttttgc tcgattcgat tcttattgat cgaaacaaca
    40981 gcaatttcgg aagctgctaa aaaagcattg atcaatgtca aaacgatcaa taataaaatc
    41041 tgcgctaaca gcgactgact ctcagggtca gcattcataa tcgcattctc tccttataaa
    41101 attaattttt agtttacgat agaaaaaaac gtggcactat agccacgtta aaatggtatc
    41161 acaaagcaat tttctttgca ataaaaacaa gtgtactaaa actacctacg aggtcgtagc
    41221 aattttcttc ttgttttcat tttgcttgtc ttctttgacg attttgatta tataggaaat
    41281 gatcgtcttg cagattgcgt aaaaaggaac acctaagaag ataccaagca atccggcaat
    41341 atttccagct actagaagaa cgataatgat cgttaatgga tggattttca gtgatttccc
    41401 aattacatta ggataaatga tattcccatc caactgttgg acgattagca caaccacaca
    41461 acaaagtaat gcttttaccg gatcattgaa gactgttact agaaatgcag gtgctagtcc
    41521 tagataaggt cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc caaataagaa
    41581 agcataatcc acaccaatta ccatatatcc taaaaatgtg aaggtaccaa cgaataaaca
    41641 ttcgattgct tgtccgctaa tgtaattagc caatgtttta ttcagttgcc ccaataactc
    41701 cacaatttgg tttcttcttt tttctgggaa aaactgttgg atgtttggca ctagtttttc
    41761 tccgtctttc aacatataga acaagatgaa tggtgctgta atgattacta cggttgccga
    41821 agcgatcgtt ccaacgatcg aacctaaact attggatact ccactaagga attgctgaat
    41881 gattgtcccg taagaaatgt ctaattgctc aaaatattta ttgatatcta cttccttgaa
    41941 caaaggcaat tcggctacct cttttaacca gctttctacc tgtttgatga aatcaggcat
    42001 attagatgcc aatgaggaaa tttgctgtac tagacttggg atgacactaa gtaaaatcca
    42061 aataattgct gcaactaaaa ggagcagcac aattgcgaca gcccatattc ttttgatttt
    42121 tgctttcatc aacagcttga caataggatt caataaatag taaagaaagc ctgcaatcag
    42181 tacaggtgca aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa agtttatttc
    42241 tgatgaaatc atgatcaatg cagctatgat caataattca agcgaccaaa acattagttt
    42301 agactgtcta agtttttcaa acaatagatt tcctcctttc tttcttacta gtatatatta
    42361 tattcgttaa aaattctttt attctttttt tatttttgaa aacttgccat actttttatt
    42421 tgttttattt tccagtatac tatacgaaag gaaggtggct agaccatgaa agttgtacaa
    42481 acaaaagata caatgagcaa tatttactta gatgctgtac ggatcaggca tcaagttttt
    42541 gtagtcgaac agggtgtgcc tttgtctcga gaaatagaca aagatgaagc ccattgtatc
    42601 cacttcgtcc tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta
    42661 gaaaacggaa aaatgaaatt acagcggatg gcgattcttt ctgaatacag acaccaaggg
    42721 ttaggaaaaa tattgattga agaagcagaa aactttgcta agaatcaagg ctataatacg
    42781 attttattag gggcacagtc cacagctgaa actttttacg aaaaactagg atatacagct
    42841 tacggcgatc cttttgaaga tgctggcatg cctcatattt atatgaaaaa gactttataa
    42901 aaatttattt cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc tcactggctt
    42961 actgttcttt tttgtgttta cttacgcgac aattgcttgt aacggttata ccaaatatca
    43021 atatagcctt ccgagaacgg gccatctcca ttattgatcc agtcaaccag aatcttcaca
    43081 ttttctttta ggatgaagtc aatatcgttc gggtaattca tcattttgct gtgcatctca
    43141 tattcatcga catccaatag tcgtttctcg ccgtcaggaa ataccttgat atccaagtca
    43201 tagtcgatat atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag attacaataa
    43261 taggaaaccc ccttttctct gatcattgct attacattga accagtattt tttatgaaaa
    43321 taaacaatcg ctggttcgcg cgttacccat cggcgtccat cagattcagt gactaatgtg
    43381 tgatcattca cgccgatcat agagtgttcg cttgttttta ataccatggt atctcgccac
    43441 gttcgatgca atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc tccttctttt
    43501 ggaactccca tagcaaccct tccttaagca gcattttgtg ctttgtgttt gggtgttcat
    43561 tacaccgaca tcataattat tatatcaata aaaatgagct ttgcctagaa aggattccta
    43621 ttgtttcgat ttttctctaa ctgatctacc agtttcattt ggaccttagg aaaaacgacc
    43681 gaatcaaatg catcaggctt caaccacttc gaagttttgt tctcggtaaa ttcttgttct
    43741 gctccctcgg ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg actaaaaata
    43801 tgagtaactt ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt ttcaaatggg
    43861 agattttgct cttcttctgc tactaggtca tcaaacagat cgatttctgg ctttgtttcc
    43921 cattcttttt tcaatcgctc atactcctct tgtgtcactt ccatcattgg aaatgtccac
    43981 atattcgcca gcaatttttg gctatctctt tcttcaaaat aatacgctcc agaatgattt
    44041 tgaagagctg cactgatata ataaacgtct tttggctttg ctttttttgt ttttacagga
    44101 aaactcgttt ggattcctcg tttgttagct aaacaaaagg cttggatcgg acaagtctca
    44161 cactttggag aagttggtgt acagatagct gaacctagat ccatcattgc ttggttgaat
    44221 tcacctggat gcttttcatc aatgattttc cgcatcgctt catcaaagat tttcctgcta
    44281 gaagcttttg cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg catcacgttg
    44341 ccgtcaactg caggttctgg aagtccaaat gcgatacttg caatcgcgcc tgtcgtatat
    44401 ggtcctattc ccttcaatga gctaatttct tcaggcgttt gcggcatttc cccatcaaat
    44461 tctgacatga tctgtttagc agcagcttgg atgttccggg ctcttgagta ataaccaagg
    44521 ccctcccatg cttttaaaag cttttcttct ggagcatttg ctagctcttc aattgtagga
    44581 aaccattcca tgaaacgata aaaataatcg atcactgtgt ctactctggt ttgctgaagc
    44641 atgatctcag agatccagat acgataagga tcacgattat agcgccaagg caggtttctt
    44701 ttttcttgtt cataccattg aatgaactga tcttgaaatt cttttgtttc tttatctgtc
    44761 catttttccc attctttatt cactttcctc ttccttttcc gtaatgaatc gctggatcat
    44821 atccaaatca aatccttttt gatataagct ttgctttact tttagatttc tttttttagg
    44881 ttcaaacctt aaatttcttc gccatagttt atctccttgt ttgactaaat aatcatattc
    44941 tatttcttga tctgcttctt gcggaagatc agctatcgct tgttggatca cttcttgaga
    45001 aaagcctttc gtcatcaatg tttgttggat ttttctcaat aattcttttt ggctttttcc
    45061 gtgatttttt ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt ggtcttcaaa
    45121 tgtatattgg ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt gctgcatctg
    45181 ctgcgctaat ttctttggtc ctttgtcact taagcgcata ttcgtgcgca cataactttc
    45241 cccatagacc aaatcatcga caaggtccaa ttcttttaag cgggctacga ttttatttcg
    45301 atcttcttga aggatttcct tttcttttaa gtatgttcgg atttcttttt ccgatcgcaa
    45361 ctgataactt aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc tactcttctt
    45421 gatctcttgg atagtttctt cgtctaattc ccgtcctttt aataatcgat agcgtactaa
    45481 tacatcttca gatacttgta aagcttctcc ttcagataaa tctacgcgat agaaagggcc
    45541 tttgccttta ctgattctcg taacggttaa cataaacatc ctcctcgaac atatattctc
    45601 tatcatacca cagaaaatcc ccgtaatcag gtattatatg ttagaataaa ccacagaaaa
    45661 aagaaaaagg gagatactta tggcagaaac aattgtcaaa actggacaaa cggttccttt
    45721 gaaaatcaag cgtttaggca taaatggtga aggaatcggt tatttcaaac ggttgatcat
    45781 atttgttcca tatgcactac caaaagaaga agtactagtg aaaatcacga aagcaactcc
    45841 tcgctatgca gaaggagaat taataaaagt caaaaaaaca agcaaagatc gcgtagtggc
    45901 accatgtcct gtttactatg aatgcggtgg ctgtcaatta caacacttag cttatcaagc
    45961 tcaattggat tttaaaaagg atctgctttt gcaagcattg gaaaaattca aaccagcagg
    46021 ctttcgatct tatcaacttt tgccgacgat cgggatggat gagccttggc attatagaaa
    46081 caaagcacaa tttcagttac gaaaaaataa gcagactcaa aaaattgaag ctggtttata
    46141 cgaagcaaac tctcatcagt tagtccctat aacagactgc ttggttcaag aacctcgaac
    46201 acaaaaagta atgaatactg ttgttcaact attgaataaa tttgatgcac ccatctatga
    46261 tgaacggaca aacagtggaa tcttccgcac aatcatggtt agaatcggtg ttaaaactgg
    46321 ggagttacaa gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa acgcaatgat
    46381 ccgtgagatc aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg tccaggcaca
    46441 aaaaacgtct ctcgtgatgg gggatgaaac gatccactta tggggaaaag aagccattga
    46501 agaacaaatc aatgaagtga ctttcgatct ttctcctcgt gccttctttc aactcaatcc
    46561 tcagcagacc gaattgcttt atggcgaagg aatcaaagca ttagatgtcc agcctaatga
    46621 aacgatcgtg gatgcttatt gcggagttgg gacgatcggc ttaagtgtag caaaacaagc
    46681 caaagaagta cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa aaaaaaatgc
    46741 ggaaagatta ggatatcaca acactcgata tgaagtcggg acagcagagc aagttctgcc
    46801 taaatggttg aatgaaggtt ttcagcctga tggtatcatc gtagatccac cacgtacggg
    46861 tttggataac gcattgatcc aatcattatt gaaataccct ccaaaaaaac ttgtttatat
    46921 ctcctgtaat gtttctacac ttgccagaga tctggttaat ttaagtaaaa agttcaagat
    46981 tgaatattta cagtccgtcg acatgtttcc tcagactgca cgttgtgaag ttgtcgtgaa
    47041 gatgacgaga ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc tgtatcaagt
    47101 aataagaaca gccaaacaaa aatagctctt catgttttat gaatactgta cttgtgaccg
    47161 aagagttaac aggaggacac tatttattta aaaaaagggt tgtgacatag tttttgtcac
    47221 agcccttttt gattattatt ggatattcac gtctatcact tgataaaatg catttgtcgt
    47281 atcgctgata ttccatactg ctaaaatcac attatatcct tttcgatcct gcggaagtgt
    47341 gatcgtttgg ttgactgttt ttggtggaac agttgctttg tcgtctattt ttgtgatcaa
    47401 ttcaaaatca ctaaatttca aaggttgatt aggattccaa gttggtttgg taatgtagta
    47461 atcccaagta cttgtccgat gttgtgctgt taaattccaa gtaatattta atgttcctgg
    47521 attaaccatg tttttatgcc atctgttaga tgtctgttca tctaaaggct caaagccgct
    47581 gactcctgca ctagcaattt ttccatcaat gaatgtattt ttggtagctt cgatactttg
    47641 cggttcccat tgtgctcttc cgacattttg atttaagttt cctgctgctg atgttcctag
    47701 gtatgcacgg cttcctggag aagtaatata accatgcgca aaacctggaa tgctaaaacc
    47761 gaacaaccct aaactaacta acccaataaa caataacgta ctaatctttt tcatgtagaa
    47821 acctccttca tttgataacc acattgtaaa cgctttattg tattaacaca aacattatgc
    47881 ttttataatt ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga ttataacaga
    47941 agtagacgag aaaagtacaa atagaaaaca tgagaatcaa atcatatgat ctttttcaaa
    48001 aacatcattc atataaacaa atgttcaact ttttaataac aacaattatc aatttttttc
    48061 ttacttcaca aactaaatac aggtcttgct gtgagccttc atggtaaact aatattatag
    48121 aaaggagtta gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg tgttatagga
    48181 attgtcatat taggcttttt atcagggttg cttagcggaa atcccggaaa atattactat
    48241 tctttgcaac ttccaccatt tgcaccacct agctggatat ttgggccgat gtggacctta
    48301 ctgtatatat tgatgggtat ttctctttat ttgttactta atcacaacaa caaaaaacag
    48361 cgaaacaatc ttgtcggtct ttttgtgatt cagttcattt ttaattttat atggtctgct
    48421 ctatttttta atttaagaaa tattttcatt gcagcaatcg atattacact tttagttatt
    48481 ttcttgtcag tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat gtggctcatg
    48541 attccttatt atttatgggt actattcgca actctattga attattcaat ttatttttta
    48601 aactaagcgc taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg ccacctaagt
    48661 tagatttttt gtctaattta ggtggcactc tttattaacc aaactatttt tttctagtac
    48721 ccacttgttg ttccatactc attattctgg tcataaccat aaccattttg ttcctgtcca
    48781 gcgtaaggat cataactttg agtatcttga cttggtgtat agtactgact tgtatcttct
    48841 gtttgctgat aataggtatt cggatcttgg tactgatcat atgtgttttc tgaataggaa
    48901 gaataatcgg tgtacccttc actattcgat ccatcaccgt ttgtcgaatc gccatcttcc
    48961 gaatcatcaa agaacatcat tgaagcattc tcatttttca aattaggtaa tgtatcgctt
    49021 gtttttatat ttaattgttt cttcagttca ttttggatcg aaagaagatc attttttcct
    49081 aaaatttgat agtagatatc attgatcatt tgtccttcgc cttgcaactg gtcttgtttg
    49141 attgtttcaa acgccggggt gtagttcgtc gctacatcaa gcatgtcatc ccaatctaag
    49201 tccgtcgtca cattgttctc aactgctttt aaaatttttc tataattgct tacgccatct
    49261 aaacttaaaa ctttattgac gattttcgtc actacttccc gctgacgacg ttgtcgaccg
    49321 atatcgcctt ctggatcttc gtgtcgcatt cgagaatagg ctaaaccttt ttctccattc
    49381 aatgtttgtt cacctactgg tacatggaca ccatccaaag taaagtcaat tttattattc
    49441 actttgatgc caccgaccgc attgatcaaa tcttccatgc catccaaatt gatcgttaca
    49501 taatgatcaa taggaatatc gagcagttgc tcaattgaat ccatggccat ttcgacacca
    49561 ccaaatgcgt aggcatgatt taatttatct accgttccat aacctacgat attcgtataa
    49621 atatctcggt ccaaactgat gatcgttgat tttttttgtt tcggattaac tgttactacc
    49681 atcatcgtat cagaacgtcc ttgttccgtt cttcctaatc caccggtatc taaacctaat
    49741 agcaaaaccg aaaaaggttc tttgtcatca atgcttactc tctcagaacg tttggaggat
    49801 attcgattag agtttttaga aattttgttt actgtttggc tcgcttctcc cataaatctg
    49861 attccgtatg ctgataatcc ggcaatcagc aaaacgaata cccctaacaa ggttaataga
    49921 actttttgcc atcgcttcat aatacacctc tgtttcacat tttctaaaat attgtctcat
    49981 ataactgttc aatcagcaaa ttcacttttt aatcataaga aaactttaaa aaacattttt
    50041 gtgtaacgat tattattttt taaaatttct caaaattctt aactatcata ccataccgtg
    50101 aaaaagatcg aaagaccgaa tacacgcagt tcgcaatcgt tttatctttt tttatgtatt
    50161 ttttccagaa aaagcgaaca acaaggtatt ttccaaataa tcgtgcagta aaatgatcgc
    50221 acataccttt cccatctttt taacagtctt tgcctaccgt tcagattatc attcgatttc
    50281 acgacaattt cttcctatga ctggtccttt tgttaaaccc atcttctatt cgttttattt
    50341 ccagctcact gcatggctct taaaatgact cgaactagtg attgcattac catcatcttt
    50401 taatggatag atatgctcct cttcaaagta aaacgatttt cttttaaagt aatagaattg
    50461 acatggtaat gagtatgtat actagtggtt tcacaaatgg tcttttattc aacatccaaa
    50521 aactgaaatt tctttaaaat ccattactaa cgaagaaaat gttccttgct tgataattag
    50581 ttaggacatg tcaagtgaac aaatgcattt acttagccaa aaaatctaaa aacttcataa
    50641 atagacaaaa agacacgact ctctaagaag tcgtgtcttt attcaacaaa ttattataat
    50701 ttagtcactt cagcagcttg aggtccgcgt gcaccttcaa caatcgtgaa ttctacttct
    50761 tgaccttctt ctaggctctt gaagccgtct ccttgaattg ctgagaaatg aacaaatacg
    50821 tcatctccgc cgtctactgt aataaaccca aatccttttt cattgttgaa ccatttaacc
    50881 gttccgtgtt ccataaatgt atagcctcca aaaaagttct tgcaagtaac cttgcaagaa
    50941 cattataccc aatttagaaa acgctagcaa gctttcgctc ttttattttc caaattattt
    51001 ttaagactga tatgacggag gaaatggtta ttttgctgct tcgaaacgtt tgtttacttc
    51061 atcccaatta ataacattcc agaatgctgc aatataatct ggtcggacat ttttatattt
    51121 caaataataa gcatgttccc aaacgtccaa acctagaatt ggtgtctttc cttccatcaa
    51181 tggagaatct tgatttgcag tagaggtaat agctaatttt ccattttcca ttacaagcca
    51241 agcccatcca gaaccaaatc gtccagcggc tgcttttttg aattcttctt taaaagaaga
    51301 aaaatcacca aaagcttcat taatcgcttc ttttatttct cctgtaggtt cgccaccagc
    51361 atttggtgcc atgatttccc agaaaaatga atggttagca tgtccgccac cattgttacg
    51421 tacagctgtc ttgatatctg ttggaatagc gtccatatca gataataatt cttctattgt
    51481 tttttcgcct aattctgggt atttctcaat tgctgcattt aaattcgtca cataagtatt
    51541 gtgatgctta tcatgatgca gatgcatcgt ttcttcgtca atataaggtt ccaatgcgtc
    51601 atatgcataa ggtagatctg gtaatgtgta agtcataaaa aaattcctcc tcaatgtttt
    51661 cttaataatg tctacattac aaagagtaac aaagagaagg aaattcttca attgttatgc
    51721 ctagtcgtct ttttcacttt gacctacttc ttcttcaaaa acctcagttt ctttaaaaat
    51781 aaacaacttg ctgaaaatat agttcgcaag gataacaata atttgagtga tgattttcgc
    51841 cacatactca ttgctgttca taacattgat caacaatacc attgccccca tgtccaatcc
    51901 gtaagaaagg atacggtaga agaagaactt accgcattca ataagtgcat gtgtaaaatt
    51961 cggtgatttt gaatggaata cccaaagttt gttcgttaca aaagcaaaca atacagaaca
    52021 aaaccacgaa atagaattgg ataaggccat acttaaatca aaatattggc gtgcgagagc
    52081 aaaactaaca aaatttacaa tcgtagccaa accaccaaaa aagagataga ctagtatctc
    52141 ccaatatcct tttgcttcta aatatccttt taactgtcga tactgcttca tgagccaacc
    52201 taccttatta aaaattctta tttatcatac tctatatttc aataactttc atctttattt
    52261 aaaaaaatta attttttttg atcctttgcc tttgtctctg cttggatcgt cacatgtgta
    52321 ataccatatt ttaattttaa taaagaaatt atacgaggat atatcttttc aatctcgctt
    52381 agtagctgat cttcaaaatc aatatgtccg tcgaaaataa ttatcttttc gctatactgc
    52441 caaatatgga catgatgaac attctttacc ccatcgattg cttcaatttc ttgttccatt
    52501 ttttcatagt caatagcagg tgcactttgt atcaaaatag cggcagcttt tttgatcacc
    52561 attatcgctt ctatcaatat atatatcgaa atgatcagtg taataactgg atcgatccag
    52621 tagatatcga aaaaacggat caaaattgct ccgataatca cacttaccga agacagtgta
    52681 tcacttaaca aatgcaaata actagatttt atattcaaac tttcatctgc ttcttgtcgt
    52741 aataataata ctgagaacaa attcgcgagc aatccaataa ctgctactgt tagcatcaaa
    52801 tgactattta tcttttctgg tgaagtgaac cgcttgaatg cttcaacggt taaaaacaag
    52861 gataatatga tcaatatgaa tgagttgatg aaagcactca atatttctaa acgctggtag
    52921 ccatacgtac gttttttatt tgcttctttt cgggccattt tttgtgcccc gtaagaaaaa
    52981 ataattgcca atgtatcact aaggttatgg aaagcatctg aaacaagaga caaactccct
    53041 gacataatcc ctccaataaa ttctgataaa gtgatcaccg cattcaaaac cactactcca
    53101 aatagttttt ttgatgatct ctgtggctgt gtaggcacta ttccttcctc cattttattt
    53161 atattggata gtctttagac aagaataaca tttttttgat atttcaccaa tcattcagat
    53221 gtttccctaa agcacttgta tttcacttga tacttcgcta gaattgcaag tgatgacact
    53281 actagatgag gagtacttat gacaacaaaa caattaatta tcagttcctt tatacgattc
    53341 aaagacctaa aggagacgag aaatgtccca ttttggaaag ccattgtcta tcttttagtt
    53401 ttgagtataa ttatggcttt gccaatctct tttcaagttt ttcaagtctt aaacaacatt
    53461 caattggatg ggcaaaagat tgctactcgc attcctgatt ttacaatcca aaacgggcag
    53521 cttcaaaccg aagaaaaaga aggattcatt taccaaacaa actccattat cttcacattt
    53581 gatccagagg gaaaacgaac agaacaggac atctctactg atttgatggg gaattttgtt
    53641 agtgtcggga tgttaaaaaa caaacttatc atagctcttc ctaatacagg cacgacttct
    53701 gctttgttga acaataacca attagaactt ccctacacca atgaatcttt aaaaaactta
    53761 acaggtaagg agctccgttc ttttttaagt aaagcatcta taccactttg gataaaagtc
    53821 ctgacattta tattttctat ttatcctagt tttctgaatc tgctgatcac tttgctattt
    53881 accaacattg ctgcttttct ctatgcacgc tttcgactaa caaaagctac ttttcttgat
    53941 tgtttaaaaa cattgatcta ttcaatttca ttgcctacta taattgcaac aatcttgatg
    54001 gtctttcttc cttcttttga cacaagcgcc ttcattgctt ttgcaggaat ctttattttt
    54061 gctcaagctg tcaaaggatg ggcaaaaata tcgatcaatt aagaaataaa gacaactcgt
    54121 tatttctact tcaatagacc cttttagagg ttgtgacatt tttttgtcac agcccctttt
    54181 ttatttgaaa aatatttctt tctcaaaaaa caagcaaggc gcttcaaagt tcttcttgta
    54241 tgcgctacac ttagagtaaa gaaaggcggg aaattaacta tgtcagaaag aatcatttat
    54301 gcagatcgtc catttcgtga tgaatttatc gaagaaatcc atactatagc gcctggttat
    54361 atctttaaaa cagaattaca accagaggat ctacaaaatg tagagatcag tttgggctgg
    54421 aactcagact accaagaaaa actattagcc tcttctacct tgaaatgggt ccaatctatc
    54481 tcagctggtg ttgatcagct accccttgag actttttcaa agaaacatat tttactttca
    54541 aatggtagtg gcatccacag tgaatcgatt acggagcatg tcttaggaat catccttggg
    54601 tattctcgtg gtctttttca agcacaaaaa gcacagagtg acatgaaatg gttaggttca
    54661 tcggtccatt accaatctat ccaaggaaaa aaaatactaa tcattggaac tggccatatt
    54721 ggaaaaatga ttgcaaaaaa atgcaaagca ttagacatga actgctatgg aatcaataca
    54781 tccggtcatc cagcagaagg aatggaaaag actttttctt taaatcaact aaaggaagtt
    54841 cttcctgaag tagatttcgt agtcaatatc ttacctctaa cggaggaaac aactgggctc
    54901 tttgatcagc atatgtttga acactttgat ccaaaatctg tattcatcaa tgttggccgt
    54961 ggtgcttctg ttaagacaca agatctggtg caggctttaa ataaccagca actttccttt
    55021 gcagcattag acgtttttga ggaagagcct cttccacaag atcatcctct ttggaaaatg
    55081 gacaatgtac tgattacttc ccacattgca ggtttgacac ctgattttca aaagaaatta
    55141 atggctattt ttttaaccaa tctgaaaagc tactttgcca cccatgagct tcaaaccaat
    55201 caagtaaaat tgtctgcagg atattaatag ctgaaaacaa tattttcaaa agaacaaaga
    55261 gaaggtgaca aaa
//
LOCUS       DACDYM010000008        54726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDYM010000008 DACDYM010000000
VERSION     DACDYM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.008"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            156..1415
                     /locus_tag="IWM59_000514"
     CDS             156..1415
                     /locus_tag="IWM59_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9978572.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            1421..2002
                     /locus_tag="IWM59_000515"
     CDS             1421..2002
                     /locus_tag="IWM59_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9978573.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(2084..2959)
                     /locus_tag="IWM59_000516"
     CDS             complement(2084..2959)
                     /locus_tag="IWM59_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9978574.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            3107..3439
                     /locus_tag="IWM59_000517"
     CDS             3107..3439
                     /locus_tag="IWM59_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9978575.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            3551..4759
                     /locus_tag="IWM59_000518"
     CDS             3551..4759
                     /locus_tag="IWM59_000518"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9978576.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            4913..5368
                     /locus_tag="IWM59_000519"
     CDS             4913..5368
                     /locus_tag="IWM59_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978577.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            5730..6005
                     /locus_tag="IWM59_000520"
     CDS             5730..6005
                     /locus_tag="IWM59_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978578.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            6018..8096
                     /locus_tag="IWM59_000521"
     CDS             6018..8096
                     /locus_tag="IWM59_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9978579.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            8256..10142
                     /locus_tag="IWM59_000522"
     CDS             8256..10142
                     /locus_tag="IWM59_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9978580.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            10154..11101
                     /locus_tag="IWM59_000523"
     CDS             10154..11101
                     /locus_tag="IWM59_000523"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9978581.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            11121..11648
                     /locus_tag="IWM59_000524"
     CDS             11121..11648
                     /locus_tag="IWM59_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9978582.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(11711..12364)
                     /locus_tag="IWM59_000525"
     CDS             complement(11711..12364)
                     /locus_tag="IWM59_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9978583.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            12496..13338
                     /locus_tag="IWM59_000526"
     CDS             12496..13338
                     /locus_tag="IWM59_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9978584.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            13496..14392
                     /locus_tag="IWM59_000527"
     CDS             13496..14392
                     /locus_tag="IWM59_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9978585.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            14395..15108
                     /locus_tag="IWM59_000528"
     CDS             14395..15108
                     /locus_tag="IWM59_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9978586.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            15128..15646
                     /gene="msrA"
                     /locus_tag="IWM59_000529"
     CDS             15128..15646
                     /gene="msrA"
                     /locus_tag="IWM59_000529"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9978587.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            15643..15870
                     /locus_tag="IWM59_000530"
     CDS             15643..15870
                     /locus_tag="IWM59_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9978588.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            16022..16573
                     /gene="lepB"
                     /locus_tag="IWM59_000531"
     CDS             16022..16573
                     /gene="lepB"
                     /locus_tag="IWM59_000531"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9978589.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(16656..17198)
                     /locus_tag="IWM59_000532"
     CDS             complement(16656..17198)
                     /locus_tag="IWM59_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9978590.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(17435..17833)
                     /locus_tag="IWM59_000533"
     CDS             complement(17435..17833)
                     /locus_tag="IWM59_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9978591.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            18093..18953
                     /gene="ylqF"
                     /locus_tag="IWM59_000534"
     CDS             18093..18953
                     /gene="ylqF"
                     /locus_tag="IWM59_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9978592.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            18946..19713
                     /locus_tag="IWM59_000535"
     CDS             18946..19713
                     /locus_tag="IWM59_000535"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9978593.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            19769..20626
                     /gene="dprA"
                     /locus_tag="IWM59_000536"
     CDS             19769..20626
                     /gene="dprA"
                     /locus_tag="IWM59_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9978594.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            20748..22826
                     /gene="topA"
                     /locus_tag="IWM59_000537"
     CDS             20748..22826
                     /gene="topA"
                     /locus_tag="IWM59_000537"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9978595.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            22795..24171
                     /gene="trmFO"
                     /locus_tag="IWM59_000538"
     CDS             22795..24171
                     /gene="trmFO"
                     /locus_tag="IWM59_000538"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9978596.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            24369..25274
                     /gene="xerC"
                     /locus_tag="IWM59_000539"
     CDS             24369..25274
                     /gene="xerC"
                     /locus_tag="IWM59_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9978597.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            25311..25859
                     /gene="hslV"
                     /locus_tag="IWM59_000540"
     CDS             25311..25859
                     /gene="hslV"
                     /locus_tag="IWM59_000540"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9978598.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            25873..27273
                     /gene="hslU"
                     /locus_tag="IWM59_000541"
     CDS             25873..27273
                     /gene="hslU"
                     /locus_tag="IWM59_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9978599.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            27291..28082
                     /gene="codY"
                     /locus_tag="IWM59_000542"
     CDS             27291..28082
                     /gene="codY"
                     /locus_tag="IWM59_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9978600.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            28164..29039
                     /locus_tag="IWM59_000543"
     CDS             28164..29039
                     /locus_tag="IWM59_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9978601.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(29171..29809)
                     /gene="plsY"
                     /locus_tag="IWM59_000544"
     CDS             complement(29171..29809)
                     /gene="plsY"
                     /locus_tag="IWM59_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9978602.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            29936..30370
                     /locus_tag="IWM59_000545"
     CDS             29936..30370
                     /locus_tag="IWM59_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9978603.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            30536..32581
                     /gene="parE"
                     /locus_tag="IWM59_000546"
     CDS             30536..32581
                     /gene="parE"
                     /locus_tag="IWM59_000546"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9978604.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            32593..35043
                     /gene="parC"
                     /locus_tag="IWM59_000547"
     CDS             32593..35043
                     /gene="parC"
                     /locus_tag="IWM59_000547"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9978605.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            35320..37551
                     /gene="pflB"
                     /locus_tag="IWM59_000548"
     CDS             35320..37551
                     /gene="pflB"
                     /locus_tag="IWM59_000548"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9978606.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            37795..38556
                     /gene="pflA"
                     /locus_tag="IWM59_000549"
     CDS             37795..38556
                     /gene="pflA"
                     /locus_tag="IWM59_000549"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9978607.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            38641..39567
                     /locus_tag="IWM59_000550"
     CDS             38641..39567
                     /locus_tag="IWM59_000550"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9978608.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            39749..40642
                     /locus_tag="IWM59_000551"
     CDS             39749..40642
                     /locus_tag="IWM59_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9978609.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            40776..40901
                     /locus_tag="IWM59_000552"
     CDS             40776..40901
                     /locus_tag="IWM59_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9978610.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(41035..41667)
                     /locus_tag="IWM59_000553"
     CDS             complement(41035..41667)
                     /locus_tag="IWM59_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978611.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(41815..42357)
                     /locus_tag="IWM59_000554"
     CDS             complement(41815..42357)
                     /locus_tag="IWM59_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9978612.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            42492..42881
                     /locus_tag="IWM59_000555"
     CDS             42492..42881
                     /locus_tag="IWM59_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978613.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            42885..43649
                     /locus_tag="IWM59_000556"
     CDS             42885..43649
                     /locus_tag="IWM59_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9978614.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            43778..44725
                     /locus_tag="IWM59_000557"
     CDS             43778..44725
                     /locus_tag="IWM59_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9978615.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            44855..45595
                     /locus_tag="IWM59_000558"
     CDS             44855..45595
                     /locus_tag="IWM59_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978616.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(45886..46554)
                     /locus_tag="IWM59_000559"
     CDS             complement(45886..46554)
                     /locus_tag="IWM59_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9978617.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            46688..46975
                     /locus_tag="IWM59_000560"
     CDS             46688..46975
                     /locus_tag="IWM59_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978618.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            47391..48929
                     /locus_tag="IWM59_000561"
     CDS             47391..48929
                     /locus_tag="IWM59_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9978619.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            48926..49891
                     /locus_tag="IWM59_000562"
     CDS             48926..49891
                     /locus_tag="IWM59_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978620.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            49878..51200
                     /locus_tag="IWM59_000563"
     CDS             49878..51200
                     /locus_tag="IWM59_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9978621.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      51263..51367
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            51441..52184
                     /locus_tag="IWM59_000564"
     CDS             51441..52184
                     /locus_tag="IWM59_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9978622.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            52199..53191
                     /locus_tag="IWM59_000565"
     CDS             52199..53191
                     /locus_tag="IWM59_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9978623.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            53203..53940
                     /locus_tag="IWM59_000566"
     CDS             53203..53940
                     /locus_tag="IWM59_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978624.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            53930..54652
                     /locus_tag="IWM59_000567"
     CDS             53930..54652
                     /locus_tag="IWM59_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9978625.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT        18977 a         9337 c        11449 g        14963 t
ORIGIN      
        1 ggaaaaagtt ctttttttgg cagaaaaaag caaaaaaata cttgttttag aaaaaatagg
       61 tataatagac ggataccaac atccaaacga aaaagaatta ttgagaacaa tgacggtatt
      121 tcaacaaatc gtcagtgaga taagagggaa ttgaaatgaa tacaaatagc gaaaaaatgt
      181 tgcaagcttt atcagatgaa gacttgtcac aagcacaaat ctttttagaa aaagcattaa
      241 aagaagaccc agcagatata ttagctgaac taggcgaaga attgctggct attggctttt
      301 tgatcgaagc aaaacagatt tttgaacaac tgatcaacca atacccagaa aatgacggac
      361 tgaacatccc gttagcagaa attgcaatcg aagataatga gatcgactta gcttttgaat
      421 acctggaaaa agtggataag caaagtgatt actatccgca aagcttattg gcgatagctg
      481 atttgtacca agttatcgga atccctgaag tgagtgaagc aaaattgaaa gaagcagctg
      541 ggttgcttcc tgatgaacca ttgatccagt ttgcgctagc tgaattgtat ttttcagttg
      601 atcgtttcaa agaggcagca gtggtttatg aaacattgtt ggcaagcggg ataaatgaaa
      661 tttcaggaat ctcgatgcag gaacgtttag gacaatcctt aagtatgcaa ggagaattcg
      721 aagcagctat cgtaccttta gaagaagcac tgaaagaaga aaggacagat gatcgactgt
      781 tccagctagc ttttacgaat cttcaattga aagaaaatca gcaggcaatc aactatctgg
      841 aagaacttcg agaagttaat ccgcattatc aatcgttata tctatatcta ggacaagctc
      901 ttcaagaaga agaaatgatt gaagaagcac aaacagtgtt agaagaagga atcaaagaaa
      961 atccttacca agtcgaactt tatcacctgg cctcggaaaa tgctttccgt ctgcatgata
     1021 gacagcgagc agaagaactt ttactgaaag ctttagaagt aggagacaaa caagatgaaa
     1081 cattacttac tttaagtaat ctttacttgg atgatgaaag atatgaagat gtgatacaag
     1141 tgatcaatca gatggaagaa actgctaatc cttatgcaga atggaaccta gcccatgctt
     1201 ataatgaatt agaagacttt accgttgcag ctgtacacta tgaacaggct tatcatgaac
     1261 tatcacatga accagatttc ttgaaagagt atgccttttt cttaagagaa gaagggcagc
     1321 tgaaaagagc gcaagaatta ttgacttatt atttaacact tgaaccagga gatatggagg
     1381 cactgtcatt attagatgac ctaacagaga ggtgagaaaa atgctaattg atgtacggga
     1441 aaagaagaat tttttaacgt ggatggtcaa tcatgtatct tttagccgaa gagaagtttt
     1501 ttggatcttg aattatcttg ccaatcatga agcgatttta agcaatgtcc actttgttga
     1561 aggggcgaat tttgcggatc gaggactgca gatcagtgat ctatctgttg aaggggaacc
     1621 catgaaattg tttttgcagg gaaaagaatt tacggacaca gaccagattt ttcatgaaat
     1681 ccgtttgaat tggaaaaagc ctttatatgt ggaatgtata ttcgaaaatg cttggcagac
     1741 aagagaatat ctatctatct tagaagacaa tccattttct tcttgggatt cttcagtcag
     1801 tgaagaagat actgaattaa ttgaacgata ttttcaagaa aaagaagaag aggcaaaact
     1861 aaaaatgctt tatgctcaaa tcgatcaagc actagaagat ggagataggg atgctttttt
     1921 gaaattatct gatgaagtta accgaaagat ccttttgcag acacaaaaga aaagtaaccg
     1981 ggtaagtgaa cataacaatt agttaaaaat agtatagtac taaaaaagac aaaggtcagc
     2041 tggatgtgga atctttcctc atagcctatc tttgtctttt ttcttatagt tttttcagta
     2101 atcttgtctg tggcttcaaa taagtaaagc cttgcccttc cacttcatgc acattgatga
     2161 ttgaaaggaa agctttttcg tcaatctcgt ggacgatcct cttagcttca gttaattcac
     2221 tagagctaac gaccatatag atgatttttt ttgaattacc agagaatgcg ccttctccag
     2281 aaaggaaagt cacacctctt tgcagaccgg tcatcagtag tggtgcaatc tcttcgcttt
     2341 tgttggagac gacaagtaga ccttttgcag aatatccgcc atctaagatc gaatcaatca
     2401 cacgactgaa aacatacgaa acaatcaacg tatacatcat acgtttcaag tcgatatagg
     2461 ttaatgaagc aagtaagacg atgatatcaa atgccaatag ggaacgcccc atagtgatgc
     2521 cgtaattttg ttctaaaatc cgagcgataa tatcactgcc gcctgttgtt ccacccatgc
     2581 ggtagacgat cccgcttcct acacctgcaa ctaaacccgc aagtagagaa acgatcagta
     2641 gatcatgctg taaattgatt tctagtggaa ccctttgcca gatccataaa aagaatgaca
     2701 aagaaagtgt acctagaaca gtataggcca aggaacgttt accgaggatc tttcctccta
     2761 gtaaaatcaa tgggatgttg attaataaag tcgtataagc tggatcaata ccaaataaag
     2821 ctcttaagat caaggtgatc cctgagactc ccccttctgc taaatgatta gcgatgttca
     2881 agtaaacaag accaaatgca taaatacttg ttcctataat gataaaaaga acttctttta
     2941 ctgtttttcg tttgaccata tgaattccag ctttcaatta tgtattttca cagaatttcc
     3001 atttagaaat tccttcctaa atgtagcatg gaaattcgat tttctcaatt gctcttatct
     3061 aggatttgcg ttaaagtttt ggtaagatag ttttggaggg aaaaagatgg ataaaaaacg
     3121 ttctttatac agtatgcagc aggaagtgga tgaatatatc cagcaattca aggtggggta
     3181 tttctctcca ttagctcaga tggctcgatt aactgaagaa gtaggtgaat tggcaagaga
     3241 agtcaaccac acttatggag aaaaaagcaa aaaggcttct gaaccagtta actcggtagc
     3301 agaagaactt ggtgatgttt tattcgttac gatgatcatg gctaattcat tgaatatcga
     3361 cttaactgaa gtttttgaaa aaaacatgga aaaattcaat caaagagacc ataatcgctt
     3421 tgagcgtaag gacgagtaaa caagatgaag tggtcctggc cttgaagact ctgaaacgga
     3481 cttgtcaaac agtcaccatg caactgagtc tttatctcag gacaagaaac acgcaggaaa
     3541 ggttggaaaa atgaaattaa aacaattacc tctggaatac caaaaggcaa ttccagttct
     3601 gaaaaaatta gagaatgcag gatatgaagc ttattttgta ggtggaagtg ttcgtgatat
     3661 cttgctgaac cagccaatcc atgatgtgga tatcgcaaca agcgcatttc cagaagaaat
     3721 caaacagctt tttccaaaaa cgatagatat tgggatcgaa catggaactg tgttggtatt
     3781 agaaaatgat gaacaatatg agatcaccac atttcggacg gaatcaactt atcaggacta
     3841 ccgtagacca gatcatgtcg aattcgttcg ttcgttggca gaagatttga aaagaagaga
     3901 ttttacgatc aatgcgtttg ctttgaaaga agatggagaa atcatcgatt tgttcgatgg
     3961 tctgcaagat ctagaagaac agatcttgcg agctgtaggc aatcctcatg agcggttcca
     4021 tgaagatgcc ttacgaatga tgcgcgggtt gcgttttgtc agtcaactag ggttcaaatt
     4081 agaggaagaa accttctacg ccatcaaaga aaaccacgag ctattaggta aaatatctgt
     4141 agaacgtata aatgtggaat tcatcaagat gctcttggga aatgaacggg ccgcgggaat
     4201 ccatgcattt attgaaacag agtgctacat ttgctgtccc ggtttgaaag aagaaggcga
     4261 agcactttta cgttttgctg aattaccatc cgtcccattg aaagaagaga gccaagcatg
     4321 ggcattattg atcagccaac tggatctaaa agaaaatgag attcgttcct ttttgaaatc
     4381 gtggaagtgt tctaaccaaa tgattaaaga cgttcaagca cttgtccaag gactacgaca
     4441 acgagaaact ggagctttgg aaccattcga attgtacttc ttaggcaaaa aacatgcact
     4501 gcaagtagaa gaattgatga tctattttga cgaaacacct cagatggatg ccgtaggaga
     4561 atcctatgat cgtttaccta tcaagtcatt aagcgaatta gcagtagatg gtaaaatctt
     4621 gctagaagaa actggaaaaa aacctggtaa gtgggttagt gaagctttgc aattagctga
     4681 aaaagccgtc gtagaacaaa gaataaaaaa tgataaaaaa caggttttgg attttttatc
     4741 gaaagaaaaa ttgatttaaa aaacactaga ctgtatctgc tcagctttgt tggctactta
     4801 aagtgggcag atacgtcatt agaagaaaaa acataagatg tgaccactta cataaatttg
     4861 tgctaaactg aggtagtaca agaataaatt acatcataaa ggagtctttt taatgagtaa
     4921 agaaatgaca gcgctaaaat tttattttag aaatggagaa acctggacga tcaaccgccg
     4981 tcatatcggc gatctttgga tcaaacaaat cacaacaagt tttggacgaa tcaatggcag
     5041 tgaatttgtt gaaatccatc cgtgtgctgg atttaaaatc gaaatcttcc atgaagggga
     5101 cgcagttgcg acacatgata tcaacctagg cggactggaa atgggcatgt ttaatcgcgc
     5161 attgaaatac gaagatatcg agcgaatgga aatcctttac cgaaatggca cacctgatct
     5221 tgtgtacttc ccatatctag ataaaggaac agaaggatta gacaatcaat atcaatcaac
     5281 aaaaatcagc gaaaaaacag gtaacttgta tatcgtgatc aatccagatc aacgtgtaga
     5341 agatgtatac ggggaatttt tcgaataaat cgatttttac gcaaaaatga ggctgtgaaa
     5401 gagatgtgtc ttattactac tgataggctg tctcttccac accgtttatt cagaataagg
     5461 gactgtgaca agatttttgt cgcagcccca tttttttgtt gtgataaaag aacaagaaag
     5521 gcaggaatgg gaaagtaaac atccttttgt cagaaacttt tatctttctt aagttttact
     5581 tgatatctga tactatttgg aatagaatga ggcgttgaaa aatttatgga aatttctaac
     5641 tagcaataaa cgatgaaaca atgaaatatt tttcatccaa aaagaacttt gctataatga
     5701 tggaagtttg tgtataggag atctggaaaa tgagagagag ttttgaacaa caaaaaaaac
     5761 tgttgcatga tcgctatggc gccctttcga tggatgaccg ccgtcaaata ctttgtaagt
     5821 tgcgtaaaag aaatatattg atgtatcgtc agttggaacg tctgaaacat gatctgcttc
     5881 gtttggagtc aaaacgtgta cagtgtgaat tagaggggaa ccaaacacag gttgaagtag
     5941 tcgaaaccaa aattttaaag aaaaaagaac aatttttaaa aatgcttaca caaaataaaa
     6001 aataaagggg atggacgatg gaatttgctg agttagttat tgtatttgca gtaacgatca
     6061 ccttatcgaa catagctagc cgcattttgc caatgattcc agctccactg atccaaattt
     6121 tccttggagt ggtattaggt ctgacagagt ggggacaatc aatcgatttt gaacctgaat
     6181 tgttcttagt tatgattatt gcaccattgt tatttagaga aggagaaaaa gcagatattt
     6241 cttcgattct caaaaatttc gggacaattt tgttcttggc atttggagga gtcattttga
     6301 cccttgtcgg ggtaggtgcg acgttatcat tcctgttgcc aagtgtccca cttgctgcct
     6361 gctttgcttt tggagcagcg ttaggtccga cagatgcggt agcagtcagt tctctttctg
     6421 gacgagtgaa tatccctaaa aaggcgatgc atattttgga aggagaaggt cttttgaatg
     6481 atgcatctgg tgttactgcc ttccagtttg ctcttggtgc gctgatcacc ggatcttttt
     6541 cagcagtaaa cgcggggatg agtctagtag tttctagtat tgggggagct ttgatcggct
     6601 ttttgcttgt ctggttcaaa caaaaaataa ttcatttgat cgaaaaagca tcagcacaag
     6661 atgtcacagg gtatctgctg atcgaacttt tgcttccttt tctagcctat gtattggcag
     6721 aggcatttga tgtatctgga atcattgctg cagtggcagc aggtattctc caagcttctg
     6781 gttttaggaa aatctctgtg tttgatgcag aattgtcgag cttgtcccac agtacatgga
     6841 caactattgc ttttacactg aatgcattag tatttatatt tttaggaatc gaattgacgc
     6901 aagtgttctc gccagtatgg ggagacggtt tatatccaaa cggtctttta ttagcgatta
     6961 tcgtattaat ctctgtcatg ctttttgtga tcagattcat ttctatcagt cttttttatg
     7021 tattcaaaga tggcagtaaa aagttcaaaa aacagttgaa cgagatcttg atcttaacat
     7081 ttggcggtgt aaagggaaca gtcagtttgg ctacgatttt tattttgcca ttttcaatca
     7141 ataatatgat gttttaccag cggtctttgc ttttgtttct gacggcgggt gtcatcttag
     7201 tgactttggt catagggatc attgtcttgc ctatgctgac ggaaacagag gaagcgaaaa
     7261 gcacggattt gaatgctttg atgattttag aagaggtcgt tgaagttttg cgaaaagaaa
     7321 taaaggaaat tgatcgtaat acaaaagaat ttttagccac tgaagcagtg atagaaaatt
     7381 atcaagaacg gatacgtgat ctgtacttgg aagatttaac cgatgatgaa aagcaagaag
     7441 tacaagaaat acaagcactg attttgtcga tcgaacggga tggattggat gaaagttatc
     7501 gttcgggcaa gctttcttct aatggttatc gtttttactc ccgttttctt tctcgattcg
     7561 aacattctat cacaagccag attttgtctt ttatcggctt ttggttcatt gttgtccgta
     7621 ggttaatgcg aatcgttctc catccgaaaa tgttttggca aagaagatat gctgatcgtc
     7681 agacatttat tagtgatgaa gatatccaag aaatcagaga gacatatcaa aaaaatactc
     7741 agttgattat tgaaagttta gataacttga ctgatattta tgatgaaacg atggttcaat
     7801 tttttatcca gcaacgcaaa cttgaaggaa taaaaatgaa ctcggggaat ttgatttctt
     7861 cgtggatgat ccagcaagat agtctgttta cgaaaaaaat gctaagaggt tattatcttg
     7921 aaagaaaaat cattgatgaa tacgaagttg ctgaaaaaat cacgacgttt tctgcgaaca
     7981 gctatcgcag gaatatcaat ttgttagaat cttatacgat gaacaaacca tctgataact
     8041 tttctttccg atttgcgttt cgagcaaagt caaaaaaaac gagtccaata aaataaaaag
     8101 gtaaaaattt tcaagtttta taaaaaacaa aacatatcag aacaggatcg aatcggtcct
     8161 gttctttttt tgagaacaca aaaagaacaa ataccaaaaa aattttcctg aagttcacta
     8221 tttttatgat acaatatcca agataaggag ggaacatgtt gaaagaatta aaagtaactg
     8281 acctcaaaaa aacttatggt gaaaaagatt tgtttgacca gatttctttt ttggtccacg
     8341 aaaaagaccg tatcgggttg attgggacga acgggacggg taaaacaagt ctcttaaata
     8401 ttattgccgg tatcgatagc agcgacggtg atcggcaaac agtgttttat ccaactgatt
     8461 accgtattgg ttatctttct caagcagctg aattttccga tgaattaact gttctccaag
     8521 cagtctttca aggaaacagc cccttgatcc aaactgttcg agcatatgag gaagcattga
     8581 tcgaactagg ggaaaacgga gaagaccctg atgtgcaaaa acgttatgct aaagcagaag
     8641 aacaaatgaa taaagaagat gcttggacta cagataccaa cgcaaagatt attttgcaaa
     8701 aattaggaat cagtgaatta gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac
     8761 gagttagtct ggcgcaagtg ttgattgatg aacccgattt actattacta gacgaaccaa
     8821 caaaccattt agattatgaa gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg
     8881 gcgcactatt gatggttacc cacgatcgtt attttctaga tagagtagca aaccgaatct
     8941 tcgaactttc atttggaaaa ttatacgagt ataaaggaaa ttatgaagct tacgtcttag
     9001 aaaaagcaga acgtgatcgt gtagaagtag aacaagaaga aaagcgcaag cggctttata
     9061 aacaggaatt agcttggatg agggctggcg tacaggcacg tggcaccaag cagcaagcac
     9121 ggatcaaccg tttcgaagat ctaaaggaga acttgtacca agtaaaccaa gaagatgatt
     9181 tagagctgaa cctagcgaca caacgtttag ggaaaaaagt actggaaatc aaagatggca
     9241 gttaccgtat aaatgaccaa acactgctag aacatttaga tttattgatt caatctcggg
     9301 aaagactagg tattactggt aaaaatggtg cgggaaaatc gacattgctt aatattctag
     9361 ctggcagaat tcccctggat agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt
     9421 attatacaca agaaaatgaa gaaatggcac cagataaacg gatgattgct tacttacaag
     9481 aagctgctga agaagcaaaa acagcagatg gcagtcaaat cggagtagca gaattacttg
     9541 aacgattttt atttccaaga tttatgcatg ggacactgat aggcaaactt tcaggcggag
     9601 aaaaacgccg gttatttttg ttgaaactgc tgattcagca acctaatgtt ttattattgg
     9661 atgaaccgac caatgatttg gacatagcaa cattgacgat tttggaagat tattttagaa
     9721 gttttccagg tgctgtgatc actgtttccc atgaccgtta tttcttggac aaggtagcag
     9781 ataagttatt agtattccaa ggaaacggaa aacaagaact ttattacgga aacatgtcca
     9841 gttatttact gaagcaaaaa gagacccagc agcctgctga aaaagcgaaa ccaaaaatac
     9901 agagcaagga acctgctgaa aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa
     9961 cgattgaaga tgagattgca gaacttgaag aaaaaatctc attgctccaa gaagagatga
    10021 atcatcaagg agataatttc actcgattac aagaattgca aaacgacgta tctgaaacag
    10081 aagcacaact tgaagaaaaa atggcacggt gggaatactt gagtgaatgg gtagaagatt
    10141 aggaggaaaa aggatggaac aagcgtatct tgacttagga agaaaattgt tggaagaagg
    10201 gcatcgtaaa gaagacagaa caggcacagg cacattaagc ttattcggat accaaatgcg
    10261 gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa cgagtacctt ttggattgat
    10321 caaaagcgaa ttgctttggt ttttaaaagg agatacgaat atccgctatc tattacaaaa
    10381 taaaaaccat atttgggatg aatgggcatt tgaacgctac atcaaaagtc cagactataa
    10441 tggaccagat atgactgatt ttggccatag atgtttgaca gatcctgaat tcaatgaaga
    10501 atataaaaag cagagcaagc ttttttgcga gaagatccta accgatgatt cctttgctga
    10561 aacatacggc gatcttggac acatctacgg ctatcaatgg cgtcactggg aaacaaaaga
    10621 cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc
    10681 tagaagattg atcgtttctg catggaatcc ggaagatgtt cctagcatgg ctttgcctcc
    10741 ttgtcatacg atgtttcaat tttatgttca agagggacgg ctaagctgcc aattgtatca
    10801 acgcagtggg gatgtgtttc taggcgttcc gttcaacatt gcaagttatg cgttgctgac
    10861 tcacctaatt gcacatgaaa caggtttagc ggttggcgaa tttgttcaca cattaggaga
    10921 tgctcatctt tatatcaatc atatcgaaca aatgaaagaa caattgagcc gagaaatacg
    10981 cgcatttccg actttagttt tgaatcagga aaagcagtca gtatttgatt ttgagatgga
    11041 agatatccaa ttagaaggat atgacccaca tccggcgatc aaagctccta tcgctgttta
    11101 gaatcagaaa ggatgagaaa atgttagctg caatttgggc acaggatgaa aacggattga
    11161 ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa
    11221 tgacagaagc aaatacacta gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc
    11281 cgttgccgaa tcgtcagacg attgttttga cgagagattc ttcctataaa gcagaaggcg
    11341 tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga aaaagaaaca gacggtatct
    11401 ttttcatcgc tggagggtca gctgtttatc aagaatttct gccattttgt acgatattat
    11461 accgtacagt catccatcat gtattcgatg gagatgctta ttttccacct gttgactggt
    11521 ctgattggtc actgatcaat actagtcaag gagaaatgga tgaaaaaaat cgttatccgc
    11581 atcaatttga aacgtatcag cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga
    11641 cgatataaaa aagctatgga tagactagct gttatttaga gctgtcccat ccatagcttt
    11701 atttttttta ttaagtaaag aataaaacag agaagtacat aaaacctgca cctaacataa
    11761 caaacaaatg ccaaacaaca tgcatatagc ggatagaatt caagctataa aagaatgcgc
    11821 ctatcgtgta agaaagcccg cctgctacca tcaaaataac accagtcatt ccaagagagt
    11881 gataaagagg aacagcagcc acgacacaga gccaccccat aaaaatatag atgatcgtag
    11941 atacttttga tactctctcc cgtttatgca acgtcaacgc tttatatata atgccaagaa
    12001 tcgcaaacaa ccagattaat acaaataaag tccaacctag ccatcctcgg atactcagca
    12061 aacaaaaagg agtataactt cctgcaatca aaagaaaaat cgaactatga tcgaatactt
    12121 ggaacacctt ttttgctttt gtaaaaatca atgaatgaaa caaagtagac gttaaaaata
    12181 aaagaatcat catcgatcca tagatagcat aagagacaat atggagggag gaatgcaagt
    12241 gggcaccttt tactaaaaga accactagtc ccgcgatact caatccagtc ccaattccat
    12301 gggtgactgc attaaacact tcatttaata tttcatacgt tcgattaaat ttcgtttttt
    12361 ccatgaaaac ggctccttta ctaggtgtaa ttaatgaaaa atcattattt gtctgttata
    12421 ctaaaaatag aaattgtctt acatgtagtt taacatgaat aaggcatctt gttaaagaaa
    12481 gagagagaga aaataatgac aaatgtaaaa atcgtaacag attcttcatg tacgatggaa
    12541 aaaaatgtac gcgatgcttt aggcattcgt gtcatgccgc tatccatcat gatcgacgga
    12601 acagtatatc ccgatgatga ccaactagac ggcgaaagat ttatggaaat gatggcgaaa
    12661 gcaaaaaatc tgcccaaaac aagccaacca ccaattggag aattcatctc gatgtatgat
    12721 gagctaggag aagacggcag tgaagtgatt tcgatccata tgacaaaagg cttgagtgga
    12781 acagtagaag cagcaagaca agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac
    12841 agtgatacca ctgaccaagg attatctttt caggtaattc gagcagctga attagcaaaa
    12901 gaaggtaaaa cagtagagaa aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa
    12961 ttatacatcg gaatatccac actagataac ttggtaaaag gtggaagaat cagccgaaca
    13021 actggtttgc tgtctagtgt tttgaacata cgcgtggtga tgaatttcgc ccattctgaa
    13081 ttaatccctg taactaaggg gcgaggaaag aaaacgttcg ataaatggtt taataatttg
    13141 aaaaatgaac tgaaagaatt gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca
    13201 aaagaattgg cgcaagaatt caaagagggt ttgcaagaat tattcccgga catgcatatc
    13261 cctgtactgc atacgaatcc ggttatcgct acgcatactg gtaaagacgc gtttgcaatc
    13321 atgtattatg tagattagtc gaaaagagga taagccctca gcctgaaagg cttgcagctt
    13381 attctctttt tctttataaa attaggaaaa taattgtttt cttttttcat aaagactacc
    13441 tttttgataa aatttttgat aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa
    13501 attaacgaaa cgacatttga tcctcgtttt gttcccatta ttaattgcga tcatcatcta
    13561 tgcaggttta gctgcttcta taccgaaagc tggcccacta atgaaaccag taactactgt
    13621 gagagaagat ccaaataaaa aagaaaaact tcactacata gctatcggag attcgctgac
    13681 tgaaggagta ggagatcaaa ccaagcaagg aggattcgtt ccccttgtcg caaatgatct
    13741 aaaagaccgt tataatttga ccgcggttga aacagaaaac tatggtgtaa atggtgaacg
    13801 aagtgaccag atcttaaagc gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac
    13861 agctgatttt atcacactga ctgttggcgg taatgatttg atgaaagtta tccaaaataa
    13921 tttattcggg ttaacggtca attcctttaa aaagccactg aaaaactatc aggaaaatgt
    13981 cacagaacta ttggaagaat ttcgatccct caataaaacc gcacctattt acattttagg
    14041 catctacaat ccattttatt tgaactttcc tgagatcact gacatgcaga caattgttaa
    14101 caattggaac aaaggaacag aaacggtgac aaaaaaaatg ccgaactgct actttatccc
    14161 aatcaatgat ttgctttatc aaggattgaa tcaggaagta ggtgtcacag aagaaggaac
    14221 agatcaaaca actagcacga cagaaaatct aaatggcatc aaaaataatg tgctctatga
    14281 agaagatcat ttccatccaa ataatttagg ttatcaattg atggcaaatg cagtgagaga
    14341 tcagctgatc gagacacaaa accaatggtt agagaaggag gatgatcaat aaacatgaaa
    14401 acaagatctg acgtatcttc aaacaaaaca aagaaaacaa attactggaa attcgccttc
    14461 cttatccttc ttggggtagt acttggttcg accgtatttt taggcagccg tatttttgct
    14521 aatcgggaac cagaactccc agaaatacca gctcttacgg aaagacaagg agatcctgtc
    14581 ttaacgatca attcaaataa agaaaaagtc aatcaaatca tcagcttttt ccttagtgag
    14641 tatcaaaaag atagtgatat tgaatataaa ttttatttgg aaaatgaagc actactcaat
    14701 ggtactttcg aagtactagg ttttcctatc gatttctatt tatattttga tccgtatgtg
    14761 atggaaaacg gaaatgtcca actaaaagct agaagtcttt ctatcggaac attgagtttg
    14821 ccaatcaaag atgtgatgaa tatgatcaag agaaactata aattaccaga ttggatcgaa
    14881 gtcaatacag atgatctgac tatcatgctt aggttggatc aattccgaat gcaaaacggg
    14941 atgtatatta aagcagataa aatcgactta gtaaatgatg acattcggtt tagtctatat
    15001 ttgccaaaag atacctctga tgcttcaaac aactcaaata ccaacaaaac tagcaccagc
    15061 agtactgaaa gcaagacaaa agaaaataaa aaatcatcgg atgagtagta ttagaggggg
    15121 aatcataatg gaacgagcaa tatttgcagg aggctgcttc tggtgtatga tccagccttt
    15181 cgacacgctt cctggtatcc atacagtgat gtcaggatat actggagggc atgtaccaga
    15241 tccgacttat gaacaagtaa aaagccaaac gactggacat acagaagcag tggagattat
    15301 ctatgatcca acattgattt catatgaaca attagtagaa ctttactggc agcaaactga
    15361 tccaactgac gctttcggac aattcgagga tcgcggtgac aattatcgcc cggtgatctt
    15421 ttatttgaca gaagaacagc ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag
    15481 tggacgtttc gaacagccaa tcgttacaaa aatcgaacca gcccaaccat tttatctagc
    15541 agaagaagac catcaagagt attataaaaa gcatcctgaa aattttaaaa gaaaccatgc
    15601 acgccgggct gcctttatcg aagaaaattg gaggaacgaa gggtgaatcg aagctttttt
    15661 cattatttaa tgacactgcg aggcggtggc acacatgatg ctttatctgt tttcgctaca
    15721 gaagccggta aagacatcca atttcctaag cacagctcta gctacgaaga aatttctaat
    15781 tatctggaac tgaatgtcga ctatttgccg tccatggata tctttgacga agcatgggaa
    15841 aaatatttag aaaataatca tgacatataa gagatagttt tgtactttat gatcaaaaaa
    15901 gttttttgtc caatagccta attgaaagaa ttatttttat ttcagctatc tagtcgcatt
    15961 tttttgatat ttttcttata ataaagaaag aatgaaaaaa agagggatgg attgatagag
    16021 attgacaaaa aaacaacgct atattaatcg cttctggctt gtattcaaat acttacttgt
    16081 ttctgtattc cttgcgttca tgttgcgggg atttttattc atcccggtac cagtggaagg
    16141 caattcaatg gaaaacgtgt tgaagcaagg tgatatggtt gtgatggaaa aattttcgga
    16201 gatcagacga tttgatattg ttgtttttca attagcagat ggcacgattt atatcaaacg
    16261 agtaatcggt cttccaggag aaaatgtcag ctaccaaaat gatcaattga agatcaatgg
    16321 gaaagtagtt aaggaacctt atctaactaa aaacctgaaa tcagatcatg caaatgcatc
    16381 ctatacgacg gactttacac tacaagagct aacgggacaa tccaagcttc cggaagataa
    16441 ttattttgta cttggagata atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat
    16501 caataaaact gatattctag gtaaagcaag atttgtctat tatccacttg atgaaataaa
    16561 atggatccag tgagaaacaa taaagcgtgc agtctgttga gactgcacgc tttattgttt
    16621 aatccaaaat cagtttacta ccttagtacg taaaatcaaa cagctcgttt aaacactttt
    16681 ctagcctcta aagttttgat aaatgaataa acaaccatta tttgaatagg gatttggatc
    16741 atattcctga tgaatctacc tagaaaaatt actttgaaat ctgcaccaaa caaaacagac
    16801 atcagccaag aattgataaa aaggctgact gggatcataa tcaataattg ggcagcgatg
    16861 acggaaggca ggtttatctt tttatgatat aaaaataagc cattaataaa tccaccaagg
    16921 aaagccgtga taagaaaagc agcttgaaac gttcctgaac caaatatcgt gaaatttaaa
    16981 gtgttcgaca acatttcaag caaaccagcc cagatcggtc catatagata acccataagc
    17041 gccatactta aaaatccgaa tgtgatttga aaataagcag gaccaatcgc tatcttgtat
    17101 aagatgacat caagggctaa taaaagagca gaggtcgaca acaatcttgc attcatttcg
    17161 ggcaatctaa aaacgtcttt tgcaatcaaa gtgttcaaaa tattctctcc tttgtcagtt
    17221 aataataaaa ggataccata aaatcaccaa tgtgtcatga cacattggtg atttacatac
    17281 actggcatct aacaattaaa aaaagggtga gaagactgct tttataaact gtaaaaaaga
    17341 gattgtacga ataaaaaaaa tagaggcgga gaatacttat cgtatcatcc gcctttgttt
    17401 ttttgttata aaaaacgtca gattagttat ctgcttacat cttcatccat acgggttcta
    17461 atgtatttcc gtctagatct tggacttcta atgaaaacat ttcttcttca ggcattccca
    17521 tatctacttc gtggaaagta ccaccatttt cttttgcaat ttgagcaaat ttttttactt
    17581 catccgcatt ttcaacacta aaagaaatca aagcaccact tgttttttta ttatcagcta
    17641 tttctttatc ttttataaat tttttataaa aatcatgaga gagaagcatt atccaaaaag
    17701 tgtcatccca taccattgaa ccagcttctt cagtagaaaa ttcttcattt tttttaaaac
    17761 ccagcttttc ataaaaagca acggaacgat tgacatcagc aacaggaaaa ttaacaaaaa
    17821 ccatactatt catttgtttc gtctcctttc tgttttgttc gtctatcatc tcttatttag
    17881 gaaacgctgt caaaaattta gattcatata tagaaaccaa aataaaaatt tgcatcatca
    17941 aaaaagtgaa gatgtgatag aatgaggact ggttcacttt cacttaacta atagatggaa
    18001 tttctttata ataaaaaagt gaatttatat tgaaaaagaa aaaaacaagt aaagccgggg
    18061 aatattccgg tatgaaagga atggagagaa caatgaccat ccaatggttt ccgggacata
    18121 tggcaaaagc ccgacgagaa gtttctgaaa aaatgaaata tgtggacatc gtatttgaat
    18181 tggtagacgc acgattacct ttatcttctc gtaatccaat gctagatcag atcattcagc
    18241 aaaaacctcg ccttgtttta ttgaataaag cggatctggc agatccacaa cagacaaagc
    18301 tgtggcaaga atattttcgt aaacaaggcc atcttgcatt agctatcacg gcacaagaaa
    18361 gtaaaggaat taaaggtcta gtacctgcgg ctaaagaagc attgaaagaa aaacgggcaa
    18421 gagatgctgc tcgggggatc aagcctcgcg ctattcgggc aatggtctta ggtattccaa
    18481 atgttgggaa atctacgttg atgaatcgac tagtaggcaa gaaaattgca cagacaggaa
    18541 ataagcctgg cgtaacaaaa ggtcagcagt ggcttcgttt aggtaatgag ttggaattgt
    18601 tagacacacc agggatttta tggcccaaat ttgaagatga agaaatcggc aaaaaactag
    18661 cgctgacagg cgcgatcaaa gaccagcttg tccatttaga tgatttagcg atttacggat
    18721 tagacttttt tgcccgatat tattcaggaa gaatcagcga tcgttatggt ttatcaatcg
    18781 agcaagaaga aaaattggca ccggaattac tgatggatat tacagcaaaa cgtgggtata
    18841 aagaagacta tgatcgtggc agcgaattag tcgtgcatga tattcgtcaa ggaaaacttg
    18901 gacgatacac actggatcgt tgcgaagaaa tggagcaaaa aagtgatgac tgaatccatc
    18961 caaagtatca aagaacgatt gaaaagcata cagagaatag atgatcctac actagaatca
    19021 ttaagaaatg atccacgcaa aggcgtgcag caagcactgg cttcttttga acgacgattg
    19081 aaaaaggaag cattaattaa ggaaaaatat gaagaaatgt tttgctttga aaagcaaggg
    19141 tacgcacaag gtcaccaatg gattgccggc attgatgaag taggaagagg accattagct
    19201 ggacctgtcg tggcagctgc agttatcttg ccgaaaaatg agatcatatt agggctgaat
    19261 gactccaaac aattatcaga gaaaaagcga gaacttcttt atcaacaagt tcaagaaaaa
    19321 gccctggcaa tcgggatcgg gatcgttgat cagcagacaa tcgatgatgt gaatatttat
    19381 gaagcaagta aattagcgat gatccaagca gtagaaaaat tgtccattgt accaagctat
    19441 ttgttgatcg atgcgatgac cttggatttg cctattcagc aggaaaaaat aatcaaaggg
    19501 gatgcacgat ctgtgtcgat tgctgctgca agtatcattg ctaaagtata tcgagaccaa
    19561 ttgatgaaag aatatgacgt gctttatcct ggatatggtt tctcgcataa tgcaggttat
    19621 ggaacaaagg aacacttaga aggacttgaa cgacaaggaa ttacgccaat ccatcgtaaa
    19681 agttttgcac ctgtaaaaaa atatttacaa taaaaaacag ccgcttttac gtatcttatt
    19741 agaaagagag acgaaaggcg gttttatgat gtatcaaata gaagaaaatt tattgaaact
    19801 tgcttataca aaaggaatca gtagtcaagg aaaatgggca cttgctaact ggatgatgca
    19861 gtatccttat aaagaaatcg gatttgaaga catcattaag ataggtaaaa taaccaaaca
    19921 ccgggagctg tttcgcaaaa gttggcagga aattcacgct aactggggca aaatccaatc
    19981 gaaacagtcg tttatcactt gctttgatcc caaatatccc cctcagctgc ttcacttgac
    20041 ctatccgcca attgtactgt tttataatgg tgatctatct ttactttctt gtaacatgtt
    20101 aagcgttgtt ggtggaagac acacttctgc tttagccaaa aaaacagttt attatttact
    20161 aaaaccagtg atagaagctg gatatgtgat cgtcagtggc tgcgcgaaag ggatagatac
    20221 atacggtcac caagcagcaa tctctcatgg tggacggaca atcgcagtca ttggaacagg
    20281 gatagagcaa gtctatcctg ctgaaaatcg tgacttgcaa gaggtgattg caaatgatca
    20341 tcttcttttg agtgaatatc ctcctcaatc cggcccgaaa aagcattgtt ttccaatgag
    20401 aaatcggatt atcgcagctt tgtctaaagg aacatgtgtc tttgaagcca agaaaaaaag
    20461 cggctctctg ataacagcac agcaagcatt ggatcttgga agaacagttt tttctgttcc
    20521 aggaaatatt ttaacagaac attctaccgg atgtcatcat ttgatccaag acggtgcagt
    20581 ttgcacggtt tcgggtgaag atatcttggc agaatttaaa gaataacacc atacttttca
    20641 tttgcttttc ttgacaaacg ttttctgaat ggatatgatt agctacgatt tgtatgaatc
    20701 ctcttttaat agtaggaaca cgacaatgga aaggagtcca ttcagtaatg gcgtataaat
    20761 atttagtgat tgtagaatca cctgcaaaag ccaagacaat tgagaaatat cttggacgaa
    20821 actataaagt aatggccagt gttggccata taagagattt accgaaaagt aaaatgggta
    20881 tcgattttga aaacaattat gaaccccatt atatttctat acgcggaaaa ggcgatgtca
    20941 tcaaaagcct gaaagccgca gcaaaaaaag ctcaaaaagt ttacttggca agtgacccgg
    21001 atagagaagg agaagcgatt gcttggcatt tagcgtacct acttgggttg gatctgaaag
    21061 aaaaaaatcg ggtggtcttc aatgaaatca caaaagacgc agtcaaagca gcttttaagg
    21121 aaccaagaac gatcgatgta gatttagtag atgcacagca agctcgtcgt accttagaca
    21181 gaatcgttgg ttattcgatc agtcctattc tctggcgtaa ggtcaagaaa gggttaagtg
    21241 caggacgtgt ccaatctgtc gctttaaaaa ttattattga ccgtgaaaaa gagatccgag
    21301 aatttgttcc agaagaatat tggagcatcg acggtaattt taaaaaagct cgcaagaaat
    21361 tcaaagcaaa tttctgggga atcgacggta agaaaaagaa attaccagat gcacaaagtg
    21421 taaaagaagt cactgctaga attgatggaa cagacttcga agttaaaaaa gtagaaaaga
    21481 aagagcgcaa aagaaaccct gcgaatcctt tcactacaag tagcctgcaa caagaagctg
    21541 cccgtaagtt aaactttcga acaagaaaaa ccatgatggt agcacagcaa ttatatgaag
    21601 gaatcacttt gggtaaacaa ggaacagtcg gcttgattac atacatgcgt actgattcta
    21661 cccggattgc cgatacagca aaagcagagg ccgcagcatt tattgaagat acgtatggaa
    21721 aagaattttc tagacatgat caccgaaaga tcaaaaatgc tcaaggcgca caagatgccc
    21781 atgaagctgt gcgtccaacg agtgtgcttc gtacaccaga tgaactcaaa caatatttag
    21841 ataaagatca attgaaactt tatacattga tctggtcacg ttttgtggct agtcagatga
    21901 caccagctat tttagataca atgaaagtga cattagaaca aaatggtgtc atcttcattg
    21961 cgaacggttc aaaaatcaaa ttcaaaggat tcatgcaagt gtatgtggaa ggtagagatg
    22021 acggcaaaga agagaaagaa aatattcttc cagaacttga agaaggcgat gtagtccaat
    22081 ctgttgatat tgaaccaaaa caacatttta ctcaaccacc agcacgtttt agtgaggcta
    22141 cgttgattcg tgcactagaa gaaaacgggg tagggcgacc ttccacttat gcaccaacac
    22201 ttgatacgat ccaaagacgc tactatgtaa aattgacaca aaaaagattt gagccgactg
    22261 aactaggaga aattgtcaat tcattaatct gtgaattctt ccctcaaatc gtcgatatcc
    22321 atttcacagc agaaatggaa ggagatttgg ataaaattga agaaggcacg gaagcgtggg
    22381 tcaaagtggt tgatcgtttc tataaaccgt ttgaaaaaga actgacgaat gcggaagaaa
    22441 aaatcgaaaa aattcaaatc aaagatgaac cagcaggatt cgattgtgat gtctgtggac
    22501 atccgatggt gataaaatta gggaaatatg ggaagtttta tgcttgcagt aattttcctg
    22561 attgtcggaa tacgaaacca atcgtaaaag aaatcggtgt gacttgccca gtatgtcatg
    22621 aaggacaagt cattgaacga aaatctaaga aaaacagact attctatggt tgttcccgtt
    22681 tcccagaatg tgaattcact tcttgggata aaccagtggg cagaaattgt ccaaaatgtg
    22741 acggatatct ggtcgagaag aaagtcaaag gcggtaaaca agtagtctgt gtaaatggag
    22801 attacgaaga agacatccaa aaataataga aaagaggttc tttcattgaa aatggtaaat
    22861 gttgttggag cagggcttgc cggaagtgaa gctgcttggc agatcgcaca agcaggagtc
    22921 cctgtccgtt tatatgaaat gcgtccagtc aaatcgactg aggcgcatca cacaagtaat
    22981 tttgctgaac tagtatgttc caattcatta agaggaaaca gtttggctaa tgcagtaggg
    23041 ttattgaaag aagaaatgcg ccgattgaat tcggtagtaa tcacttcggc agatgaaaca
    23101 gcagtgccag ctggtggtgc cttagctgtt gaccgcgatt ctttttctga aacaattacc
    23161 cgaaaagtca aagagcatcc attagtaaca gtgataaacg aagaaattac tgaaataccg
    23221 gaaggaatca ccgtagtagc aacaggacct ttgacttctc atccattagc agaaagcatc
    23281 aaagcattca atggttcaga tggtttttat ttctatgatg cagctgcacc aatcgtagac
    23341 aaagctacga tcgatatgga taaagtatat ttgaagtccc gctatgacaa aggagaagca
    23401 gcttacttga actgtccgat gacagaagag gaattcaaag ctttccatga ggcactaatc
    23461 aatgccgaag tggcagaact taaatctttt gagaaagaaa aatattttga aggatgtatg
    23521 ccaattgaag taatggcaca gcgaggattc aaaacgatgc ttttcggacc tatgaaacca
    23581 gttggattgg aagatcctaa aactggcaaa cgtccttatg ctgttatcca gctgcgtcaa
    23641 gacaatgctg cagcttcttt atacaacatc gtgggctttc agactcactt gaaatggggc
    23701 gagcaaaaac gtgttttccg gatgattcct gggttagaaa atgctgaatt cgttcgctat
    23761 ggtgtgatgc accgaaacag ctttatgaat tcgccagaat tgctgaaacc gacttaccaa
    23821 tcaacgaaac gcgaagatct gttctttgca ggtcagatga ctggtgtaga aggctatgtt
    23881 gaaagtgcag cgagcggact acttgctgga atcaacgccg cacgcttagc aaaagaaatg
    23941 gaaccagtcg agtttccaca agaaacagct attggaagta tggcttacta cattactcat
    24001 gcggaaggaa aacatttcca accgatgaat gccaactatg gtctgtttcc tgaactgcca
    24061 gagcgcattc gagacaaaaa agcaagatat gaagcaattg cacaacgagc tttaaaagct
    24121 aatgaggcaa taaaaacaga attagaaaaa actgcaatcc caaatgagta aggaaactcc
    24181 atgaagaggg tgagacggat agtctcgctc tttttcttgc gtttttctta agaatttctc
    24241 aaacaaaatg tgaggaattg cgccaattgt gaacaaactt agaaatattg gttaaattct
    24301 gacaatttat ttgtcttttt ataaacgctg tgctaaatta ttctctggga caaaaggaag
    24361 gtgagaaaat gacagaagcc acgtgggaac aaaaattttt gcgttatttg attgtcgaaa
    24421 gaggatattc agaaaaaacg aaagaagcat atgaagaaga cattctccat ttcaaacatt
    24481 ttttggagga atctggagac gctgatctgc tgaaggcgga atattttacg gttcgtgtct
    24541 atttgagtgc attagctgat catcgtccta gctatagcag aaattctatc agtcgcaaga
    24601 tagccagcct acgttcgttt tatcagtatc ttctcaaaga agaagtgata aaagaaaatc
    24661 cattttctta cgttcatctc aaaaagaaaa atttgagact gccacgtttt ttttatgaaa
    24721 atgaaatgca agcacttttt gacagcgtag caggagatag tcctttggat ttgaggaacc
    24781 gcgctttact ggaagtcctt tacgggtcag gcatccgttt aagtgagtgt agcagtttgt
    24841 cggtatcaga cattgatttt gattcagagg tcatgttgat ccatggtaaa gggaacaaag
    24901 agagatatgc tcctctaggt tcttttgctc aagatgctct gcaagaatat ttcagggaag
    24961 gccgccaggt attgatgaca aaatatcacg aagagcatga tatcgttttt gtgaatcatc
    25021 atggaaaacc aatcacgcct actggaattg aatatgtctt gaatcaaatg atcaaaaaaa
    25081 gcagtttgga tagtgatatc catcctcaca tgctgcgcca tacatttgct actcatttat
    25141 taaataatgg ggcagatttg cggacagtcc aagaattgct aggacatgcc aatttatcga
    25201 ctacccagat ctatgcccat gtaacaaaag aaagtttaca aaaaaactat cgttctttcc
    25261 atccaagagc gtaaaaataa ggaaaagaac aaatttagga ggaaacaaac atggtcgaat
    25321 caccatttca ttcaaccact atctgtgccg tcgaaaaaga cgggaaattt gctatggctg
    25381 gagatggcca agtaacaatg ggtgaatctg ttgtcatgaa aggcactgca aagaaagtac
    25441 gacgtatcta caatggagaa gttgttgtag gtttcgccgg aagtgttgca gatgcgttta
    25501 ctttagaaga aaaatttgaa ggcaaactaa acgaatataa cgggaatcta caacgcgcag
    25561 ctgtcgaatt agctcaagaa tggcggacac agcaatctat gcaaaaactt gaagcgatgc
    25621 tgatcgtaat gaatgataaa gaaatgcttc ttgtttcagg tacaggagaa gttatcacac
    25681 cagatgatgg tatcttagct atcggatcag gagggaattt tgcgttggct gcagcccgag
    25741 caatgaaaaa ctatgctcaa aacgacatga gtgcgaaaga aattgcagaa aatgcgttaa
    25801 atattgccgc agatatctgt atctttacta atcataatat tatcgtagaa gaactgtaag
    25861 aggtgagtag atatgtatga attaaaacaa acaccaagac aaatcgtaag tgaattagat
    25921 gaatatatcg taggacagca cgcagcaaaa aaatctgttg ccgttgcttt gcgtaaccgc
    25981 tatcgccgtt tgcagttaga tgaaaaaatg caacaagata ttacaccaaa aaatatgctg
    26041 atgatcggac ctacaggggt cggtaaaaca gaaatcgcac gacgtttggc aaaaatcgtc
    26101 aatgcaccat ttgtcaaagt cgaagcaaca aaatttactg aagtcggtta tgtcggaaga
    26161 gatgtagaat caatggttcg ggatttagta gaaaatgcga tccaaatcgt agaaaaagag
    26221 cagtacagcc gtgtatacac aagtgctaaa aaaaatgcag aaaaacgttt ggtaaaaatc
    26281 ttggtacctg gtatcaaaaa agaacagaaa caatccacta atcaatttga acaaatgatg
    26341 aatcttttta atgctagtca gcaaaatacc gctgaaccgc aagaagaagt gacagaagat
    26401 attcgaatca atcgccgtac agtcttggaa caactacaaa agggtttact ggatagtcgt
    26461 gaagtaacca ttgaagttga agaccaaaag aaacctgcac caatgatgaa caatggattg
    26521 gagcaaatgg ggatcgacct aaatgaaact ctaggtgctt tgacaccgaa gaaaaaagtg
    26581 gaacggacgg taacagtcaa agaagcttta gagttattga tcaaggaaga atcagcaaaa
    26641 ttagtcaagg atgctgatat ccatagtgag gcaatcaaat tagcggaaga aagtggaatt
    26701 atttttatcg atgaaatcga taagatcact tctaaatcac agcaatctgg agaagtatcc
    26761 agagaaggcg tccaaagaga catattgcca attgtcgaag gttcacaagt aaacacaaaa
    26821 tacgggatca tccaaacaga tcacgtccta ttcattgctt caggagcttt ccatttgtct
    26881 aaaccaagtg atctaattcc agaacttcaa ggacgcttcc cgatacgggt agaattagat
    26941 gatttgacag ctgaagattt tgtgaagatt ctgaccgaac caaataatgc tttagtaaaa
    27001 caatacgtcg cgttgatcgg tacagaaaat gtgtcagtta cgtttacaaa agaagcaatt
    27061 gaacggatcg ctcaaatcgc tttcgatgtc aaccgtgaca cagataatat cggcgcacgc
    27121 agactgcata ccattttgga aaaactacta gaagatctat tatttgaagc accagatatg
    27181 caaatgggag aaataacgat cacagaagca tatgtggatg acaaacttgg ctcaattgcg
    27241 gcaaatgaag atttaagtcg ctatatttta taaaaaatcg gaggacgaca atgacagact
    27301 tgctaacgaa aacccgcaag ataaacacac ttttgcagca aaagaatact tttgacatgc
    27361 aagcagattt gccttacgat aaaatggctg tcacactagg tggtattctt gacagcaaca
    27421 cctatatcat aggaagtgac ggagtattac taggttataa tgaacgtcac gatgtgaaca
    27481 atgaacgaat caaaacaatg tttgttgaga aacaatttcc tgaatcttat actgatacag
    27541 tggcacagtt gatgaaaact gaagctaata tcccaattac tagtgatatt actgcatttc
    27601 cggtagagtc aagagagaaa tatccatttg gtctgacgac tgtcgttccg atctttggag
    27661 ctggcgaacg tttgggtacg attatcttat ctcgtatgga acaatcgttt gcagatgacg
    27721 atctagtctt agcagaatat ggagctacgg ttgtcggtat gcagatcctt tatcagaagt
    27781 ctcgaagcat cgaagcagat gtacggagtg caacggctgt ccaaatggca atcaacacat
    27841 tatcttatag cgaattgaag gctgtacaag ctatctttga agcattggat ggagatgaag
    27901 gtcgtctgac tgcttctaat attgccgata gtatcggcat cactcgttct gtgatcgtca
    27961 atgctttacg aaaattggaa tcagctggaa tcattgaatc tcgttcgtta ggcatgaagg
    28021 gaacttattt aaaagtattg aattcccgtt ttaaagacga gttagcaaaa cacagttatt
    28081 agaagtaagg ggactggggc agatagaaat gtttgttcca gccttcccat tttaaaaagt
    28141 aagtcgaagt tggaggaatg gcaatgactg ttattattca aaatgaccag ctgatcgccg
    28201 aaatcgctga acacggtgcg gaattgatca gtctgaaatc aaaagaacat ggtttagagt
    28261 atatctggca aggtgatcca gcgtattggg gcagacatgc accggtttta ttcccttttg
    28321 ttggacgttt aaaagatgat cagtatgttt atcaagggaa aacatatcaa atggggcaac
    28381 acggatttgc tcgggatatg gattttgaag tcattgaaca tggacaagaa atggctagtt
    28441 tcatcttaag gagtacgccg gagacattag aaaagtatcc atttgatttt gaattagtga
    28501 tcagctatga attaggagga gacggcatca cggttcatta tcaagtggca aataccggtg
    28561 aaaaagaaat gttcttttct atcggtggac atcctgcttt caatgtacct cttgaagaag
    28621 gattgtcatt tgaagatttc tatctggcat tttctccatt gaaatcaaga ttacgcctac
    28681 cattgaaagg cccattcatc gatatggatc aaaagacgtt aggtcagaca aatacgaatc
    28741 tggctttgaa tcgtgaactt tttgagcaag atgccttggt ttttgaaaca aggggtctaa
    28801 atgcgtacgc cattcgaagt gaaaaatctt cccatagcgt aactttaagc tataaagata
    28861 tgccttatgt agggatctgg tctccttatc caaaagaagc gcctttcgtt tgtatcgaac
    28921 cgtggtgcgg aatcgctgac acggtgaaca gtagtggaaa cttgatggaa aaagtaggga
    28981 tcaatcaact aggtgcccat gaaatcttca agacaaaata ttccatcaca gtgaagtaag
    29041 aggttgtgac cgagaagtta gcagggaaca ccgtttacca aggtgtgtaa aaaagctatc
    29101 ctgtaagtaa taagggagtg tgacatcgat ttgtcacact cccgttttat ctctttggaa
    29161 tgaaaaagta tcaggatttt ttcttgctgc tattcaaacc aaacgggatt cgactttcag
    29221 tatggtttct gatacgcttg atattgtctt tatgccgata aaagataaag gaagaaacta
    29281 aaaaggcaat gatcgtcagt aaccaattgg gcttagccaa aatcacagga gctacataag
    29341 gaaggacgat cgtagataaa gtgatcaata ctgcactgat catactgctc aaactcacca
    29401 tacttgtaca aaataaacta acgataaaga tgatacaaga ataaacaaaa aatacaggat
    29461 taaatcctaa aagcattcct gcacttgtcg caacagcttt tcctcctttg aacccagcaa
    29521 aaatcggaaa tgtatggcca agaatggcgc ataccccaaa ccaaagaggg ttaatcccag
    29581 aaaggtggaa gatcatcggc aaacaagtag ccaatgtacc ttttaaaata tccataagca
    29641 ggacaacaat cccggctgtt ttgcctaata cacgaaatgt attggtcgtt ccagtattac
    29701 cgctcccgtg ttgtcggata tcttttttga aaaacaattt gccaatccag acaccggaag
    29761 gaatagaacc taaaagatat gctaaaataa gtaataagat caatttcatt ttttaccctc
    29821 tttcaagttg aaaactatcc tttattttag catgaatagg taacaggaac aattgaaaaa
    29881 atgaaagata caagtataat agatggaata aaaagaaaag caaggggtgg cagaaatgtc
    29941 tgtaaaaaat ccaacacaag aaacgatttt caactatttg gaagaagcaa aaaatattgc
    30001 cgtcgtaggc ttgagcaata aaccagaacg aacaagttac atggttgcac aggtattgca
    30061 agacaatgga tataaaatca taccagtaaa tccattattg gctggtgaag aaattctagg
    30121 cgagaaagtc tatgcgaatt tgacagaaat cgatgatcca atcgatatcg tagatgtatt
    30181 tagacggagt gaatttttac ctgaaatagc aggtgatttt ttgaaaactg atgctaaagt
    30241 gttctgggca caattaggga ttgaaaacaa agaagcagct gagatgctaa gaaaagcagg
    30301 acgtacagat atcgtaatgg atcgttgtat taagattgaa cttggtaagt tgaaagaaaa
    30361 ccaaaaataa aaatgaccac aaagaagaga tgatcgatag aggggaatgt cgaaaaagcg
    30421 gttaggagat aattaaaatt gatttctcgt ctaccctctt ttttttcacg accttttcta
    30481 gtatgataat cgtggtgcgg ttttgccgca aagtattatt gaaggagcgt ttgccttggc
    30541 aaaaaaagta aacaacgaat acaatgactc gtccatccaa gtattggaag gattagaggc
    30601 agttagaaaa agaccaggga tgtacattgg atccactgac agccgaggct tgcatcattt
    30661 agtctatgaa atcgtcgata atgcagttga cgaagcatta tcaggttatg gcagtgagat
    30721 tgatgtgacc atccatgaag acaatagtat aactgttgca gatagcggac gtgggatgcc
    30781 tgtaggaatg catgcttccg gaataccaac agttgaagtg atttttactg tgctgcatgc
    30841 tggcggaaag tttggtcaag gagggtacaa aacttctggt ggactgcacg gtgtaggagc
    30901 cagtgtggta aatgccttat cgaaatggct aattgtaacg atcgttagag atggcgtaga
    30961 gtatcagcag aaatttaaaa acggcggtaa acctgacggt actttgaaaa agataggaaa
    31021 aacaaaaaaa gcaaacggga cgactgtcca ttttttacca gatgatacaa tattcagtac
    31081 aacgaaattt tcatatgaaa ttttagccga acggttaaga gaatctgcct ttcttctaaa
    31141 aggcgttaaa atctctcttt ctgatcttcg tggagaagaa ccagtcaagg aaatttttca
    31201 ttacgaagaa ggaatcaaag aatttgttga ttatctgaat gaagaaaaag atacattgac
    31261 accagttgtt tatttttcag gagaaaaaga aggtatcgaa gtagaggtcg cttatcaata
    31321 caatgacggt tattcagaaa atgttttatc ttttgtcaat aacgttcgga caaaagatgg
    31381 gggaacccat gaagctggta tgaaagcggc gatgacgaaa tcttataatg aatatgcgag
    31441 aaaagttggt ttgctgaaag aacgggacaa gaatctggaa ggtagtgatt tcagagaagg
    31501 gttagctgct gtactttcta tccgtgtacc tgaaaatctt ctgcaatttg aagggcaaac
    31561 aaaagaaaaa cttggtacgc cagttgctcg taccgtagta gataacgtca tcagtgaaca
    31621 aatgggcttt tatttgcagg aaaatagcga aatgagccag atgttggtcc gtaaagcaat
    31681 caaagccaga gaagcgagag aagcagcacg taaagccaga gaagaaagcc gaaatgggaa
    31741 gaagcgtaaa aaaggcgaat ctcttctttc aggaaaattg acacccgcac aatcccgaaa
    31801 ccctaagaaa aatgaattgt atttagtcga aggggattct gctggcggat ctgctaaaca
    31861 aggaagagac agaaagttcc aagcaattct acctttacga ggaaaagtca tcaataccga
    31921 aaaagctaaa atgcaagata tcttgaaaaa tgaagaaatc aatacgatga tctatacgat
    31981 tggtgcagga gtcggtcctg agttttctat cgaagactgt aattacgaca aagtaatcat
    32041 catgaccgat gcggatacag acggcgccca tattcaagta ttactgctta ctttctttta
    32101 tcgttatatg aaaccgctga ttgaagctgg gaaagtatat atcgcgttac cgcctttata
    32161 taaagtatcc aaaggacaag gaaagaagca agtcattgaa tatgcgtgga cagatgacga
    32221 attggcagca atgattaaaa aagtcggcaa aggatatatg ctgcagcgat ataaaggact
    32281 tggagagatg aatgcggaac aattgtggga aacaacgatg gatccgactt cccgtacgtt
    32341 gatccgcgtc cgtatcgatg atgctgcgca agctgaacgt cgagtaacca cactgatggg
    32401 tgacaaagta gaacctcgaa gaaaatggat cgaaaatcac gtgcaattta cgctcgaaga
    32461 agatggcagt atcttagata aaaaagagga tactgaaatc tctccttcag tttctaatga
    32521 tttattagat gaagaacgag cagataaaaa tgaaaacaat cagttatttg aagtagaata
    32581 ggaggtcacc tgatggaaaa tcgacaagaa gtacaagaat taactttaga agaagtgatg
    32641 ggtgaccgct tcggacgtta ttccaaatat attatccaag aacgtgcact gcctgatatc
    32701 cgtgatggat tgaagccagt gcaacgccgg atattatttg ccatgaacaa agatggcaat
    32761 acgtatgaca aaggattccg taaatcagca aaatctgtcg gaaatatcat ggggaattat
    32821 catcctcatg gtgacatcag tatttatgaa gcgatggtcc gcatgagcca agactggaag
    32881 ttgcgggaag tactgatcga aatgcacggg aataacggga gtatggacgg tgatccacct
    32941 gctgcgatgc gttatactga agccagatta tccaagttaa gtggagagat gctagcagat
    33001 atcgagaaag aaacagtcga tctcgtgtgg aattttgatg atacagaaaa agaaccaaca
    33061 gtccttccag ctcgataccc aaaccttctc gtcaatggat cgacaggtat atctgctgga
    33121 tatgcaacag aaatcccgac tcataactta gctgaagtga tcgatggcac aatctatatg
    33181 atcgatcatc cacaagcgag ccttgaaaaa ttgatggaat atattcctgg acctgacttt
    33241 ccaacaggag ggatcttgca aggaaaagat gaaatcaaaa aagcttatga aacaggacga
    33301 ggaaaagtta ttcttcgttc gaaaacaaag atagaggcaa tgaagggtaa taaacagcaa
    33361 atcgtgatca cagaaatccc atatgaagta aataaagcga ccttggtgaa aaaaatggat
    33421 gagatccgtt tgaataagaa aatcgatgga atagctgaag tacgagatga aagtgataga
    33481 acaggtctgc aaattgtcgt cgaattgaaa aaagacgcca atgctcaagg aattttgaat
    33541 tatttgttca aaaacactga gctacagatc aattacaatt tcaatatggt cgctatcgac
    33601 catatgactc cgcatcaagt tggactgaaa gacattttaa gaagttatat cgagcatcga
    33661 aagcaagtca taacaaaacg tagtcaattc gatttagcca aagctcaaaa aagacagcat
    33721 atcgtagaag gattgatgaa agctttatcg attctggatg aagtaatcgc aacgattcgt
    33781 gaaagtaaag acaaaaaaga cgccaaaaag aatttagtcg acgtctttca atttacagaa
    33841 gaacaagcag aagcaattgt cacattgcaa ttataccgtt tgacgaacac ggatatcaca
    33901 gagcttcaaa aggaatccga aagcttgatt gcgcaaatca cagagttgaa taaaatcctt
    33961 tcaaatgaca aagagctttt ctctgttatg aaaaaagaat tgcgagaagt gaaaaaaaat
    34021 tattcttccg ctcgtctaac gacaatcgaa gacgaaatcg aagaaatcaa aattgatacg
    34081 caagtcttag tggcacaaga agacgtaatt gtcagtgtga cgcgagaagg ctatgtgaag
    34141 cgaacaagcc ttcgttctta tagtgcttcc aaaccagaag aaatcgggat gcgagaaggc
    34201 gactacttgc tatactcagg tgaattgagt acattggacc atgttcttct gatcacaaat
    34261 aaagcaaatg tgatctaccg ccctgtccat gaactacccg atttgaaatg gaaagatgct
    34321 ggtgagcaca tttctcaaac aatctccaat ctgtctgtag acgaatcgat tttagctgtt
    34381 ttcccatatc aaaaaattca agcagagaaa acatttgtat tcatcagcaa gaatggattg
    34441 atcaagcaaa cacgaatgac agagttcgaa ccatggcgca cctataagag ccgtccgctc
    34501 agctgtatga agctaaaggc ggaagaagat gaactgattg ctgtgtatct gaaagaagat
    34561 cagccaggct tagatgtttt cttagtaacg catcaaggaa tgggtttgcg ctatccttta
    34621 acagatgttc ccgttgttgg agcaaaggca gctggagtga agtctatgaa tttgaaagat
    34681 ggagatagga ttatcaacgg gctgcttgtg ttagcagaag gtgatacgcc gatcgtaatc
    34741 gttactcagc gaggagctgt taaacgcatg cttgctcaag aaatcagcca aacttcacgt
    34801 gcgaagcggg gagtgacggt catgcgtgag ctgaaaaaaa atccgcaccg tattatctat
    34861 atgtcggaag gaagatcaaa agaaataacc ttgatgaatc aaaaagggca gcagttaacg
    34921 attgacccaa ctgatttccc tataggtgat cgaacatcga atggatcatt tgcgatggat
    34981 gagaaaaaag gtggagaagt catcgaagtt atcgatgctc ctgagatttt catcgagtcg
    35041 taactgttgt agtattttaa tgaagagacg ccatctgtat taatacaggt ggcgtctctt
    35101 tttcattcat ttttaataaa tccttatacg tttgcaaaaa aagatgaaag tgttcacaat
    35161 gcttaaatgt tggtatgtca acgttgattt aaatatatgt aaataaaaca taaaaattgt
    35221 taaataaaaa acaagaaaac ccttgcaaag atagggtgct gtgttatact aatcacgtaa
    35281 aaactgttac ataaaacaga aatttaggag gtccatacaa tggagcaatg gaatggattt
    35341 aaaggtacca aatggaaaga aggcgtagac actagagact ttattcaaaa taactacact
    35401 gaatatcgcg gtgatgacag ctttttggaa ggcccaacag aagcaacaga aaaattatgg
    35461 gatgaattgc aaaagttaaa tgatatccaa ttcgagcgta acggtgtcta tgatatggac
    35521 acagaaatcg tatcaactat tacttcacac gatgcaggct atttggataa agatttggaa
    35581 aaaatcgtcg gtctacagac agacaaacct ttgaaacaag cattcatgcc gttcggcgga
    35641 atcaatatgg ctaacaatgc gctaaaaagc aatggctata caccaaacga tgaattgaca
    35701 catattttca ctgactggcg caaaacacat aatcaaggtg tatttgacgc ttatactgct
    35761 gaaatgcgtg ctgctcgtaa aaacaaaatc attacagggc ttccagacgc ttacggtcgc
    35821 ggacgtatca tcggtgacta tcgtcgtgtg gcattgtatg gaatcgacta tttgatggaa
    35881 caaaagaaaa aagatcatga tatgactggt tatcatacaa tgagtgaaga agtgattcgt
    35941 ttacgtgaag aaatcactga acaatatcgt gcattagcgc aaatgaaaga aatggctgcg
    36001 aaatatggct atgatatttc taaaccagct gcaaatgcaa aagaagctgt ccaatggcta
    36061 tacttcggat atttagcagc aatcaaatca caaaatggtg ctgcgatgtc aatcggacgt
    36121 atctcagcat tccttgatat ctatatccaa cgtgatttgg ataatggagt aatcacagaa
    36181 aaagaagcac aagaattgat cgaccactta gtaatgaaat tacgtatggt caaattcgcg
    36241 cgtacacctg aatataacca attattctca ggattcccta tctgggctac tttatccatt
    36301 gcaggtatgg gattagatgg tcgttcatta gttacaaaga atgatttccg tctgcttcat
    36361 acattaacaa atatgggacc ttcaccagaa ccaaacttga ctgttttgta ttctgaacac
    36421 cttccagaag gattcagaac atatgctagc agaatcgcta tcgaaagttc ttcgattcaa
    36481 ttcgaaaatg ataatcagct tcgtgaacaa tggggttctg atgattgtgc gattgcttgc
    36541 tgtgtatcag gaacagttgt cggaaaagac atgcaattct ttggcgctcg tgctaacttg
    36601 gctaaaacac tattatatgc catcaatggc ggagtcgatg aagtgactgg cgcccaagtt
    36661 ggaccagaat atcgtccaat cacatcagaa atactagact ttgaagaagt gaaacataac
    36721 ttcatgaata tgatggattg gttagcagaa ctatatgtca atacgttgaa catcattcat
    36781 tatatgcatg ataaatattc ttatgaagca gctcagctgg ctttgatgga ttctgacttg
    36841 caacgcacat ttgctacagg tattgctggg atctctcatg cggcagacag cttgtcagcg
    36901 atcaaatatg caaaagtaaa agcaatccgt aatgaaaaag gaatagcagt tgattttgaa
    36961 gtggatggcg acttccctaa atacggaaat gatgatgacc gtgcagatga attagtttca
    37021 tggatcttgg aatacttcat gggacaaatc aaacgtcata aaacttaccg caactcaaaa
    37081 ccaacaactt cattattgac gattacttca aatgttgttt acggtaaaaa tacaggaaat
    37141 actcctgatg gccgtgaagc tggaaaacca cttgcgccag gagcaaatcc aagttacaat
    37201 gcggaacaaa gtgggttatt agctagcctg aactcaacag ctaaaatgaa ttacggctgt
    37261 gcattggatg gaatttcaaa tacccaaaca atcaatcctt caggtcttgg aaaagaacaa
    37321 gatacaagaa tcgaaaactt gcgtaatgtc ttagatggat acttctcaaa aggagcttat
    37381 catttgaatg tcaatgtatt cagtaatgat ttattgcgtg atgcaatgga aaatccaatg
    37441 aaatatccga acttgacgat ccgggtatct ggttatgcag ttaaattccg tgacttgaca
    37501 agagaacaac aattagatgt cttgatgaga acttcacacg acagaatgta aaaagttcgt
    37561 gaaaaaagtt tttcgactta gcgtcgaaag gacgttgaat agccatcgca tcaaaatcga
    37621 ataatcagta tgcttatttg aaagaaacga tttacagggg caggacttgt atgttccgtc
    37681 cctgtaaatt ttacacataa cgaagcggca attgcgaaaa attttgttca gaaagcttcg
    37741 cttcgacagc taacttgtac ttaaaatgaa atcaaagaaa agatgtgaac ttaaatggaa
    37801 gaaaaaacaa tcggctatgt tcattctatc gaaactttcg gttcagtaga cggacctgga
    37861 ctgcgctttg tcgtgtttat gcaaggatgc cgcatgcgtt gccaattttg tcacaatcca
    37921 gatacttgga atattggcgg agggaaagaa tacacagcag atgaactttt agataaagca
    37981 gaacgcttca gaccttactg gggagataaa ggtggtatta ctgttagtgg tggagaacct
    38041 ctattgcaga tcgatttttt gatcgaacta tttaaaaaag caaaagagcg gaagatgcat
    38101 actacattgg atacatgcgg aaaacctttt acgtatgaag aacctttctt ttcacgtttt
    38161 caagaattga tgaagtatac agatttactg ttatttgaca tcaaacacat cgataatgaa
    38221 gagcataaaa aactaacgca ttgggacaat gaaaatattt tagaaatggc tcaatatttg
    38281 tcaaaaatca acaaaccggt ttggattcgc catgtactag ttcctgagcg aagtgattat
    38341 gatgaatatt tgatccgact agacaacttt attcaaacct tgtcaaatgt agacagagta
    38401 gagattcttc cttatcatac gatgggaaaa tacaagtggg aaacacttgg tttgaaatat
    38461 ccattagaag ggatcgaacc acctacaaaa gaacgtgtcg aaaatgctaa acgattgctt
    38521 catacagaag agtatacaaa atatttaacg cgataggtcg catcggaaag aagataagta
    38581 ttgcttcttt ccgttttttt gctattatta aataggagaa atcacaaagg agcgaatgac
    38641 atgtcaaaaa ttttagtttt tggtcaccaa aatccagata cagatgcaat cggggcagcc
    38701 attgcgtttt cacacttgca aaatgaatta ggtaaagaaa cagaagcagt tgctctaggt
    38761 acacctaatg aagaaacaca atacgcatta gaccactttc agctatctgc accacgagta
    38821 gtagagtcag ctaaagcaga aaatgcagat gtcatgcttg tggatcataa tgaattccaa
    38881 caaagtattt cagatatcag cgaagtaaat attttagctg tagtagacca ccatcgtatt
    38941 gcaaactttg aaacagctaa tccattgtac tatcgtgcag aaccagttgg atgtacaagt
    39001 acgattattt taaaactata taaagaaaac caagtaacaa ttcctaaaga aatcgcagga
    39061 atcatgctct cagctatcat ttcagataca ttgttgttca aatcaccaac atgtacgcct
    39121 gaagatgaag cagctgcaaa agaacttgca gcaatcgctg aagtcgatct aaacacttat
    39181 ggacttgaaa tgttgaaagc aggaacaaat ctaagtgata aatcagctgc ggttcttctt
    39241 gatttggatg caaaaagttt cccaatgggc agttccaacg ttcgtatcgc tcaagtcaat
    39301 acagtggacc ttaatgaagt gatggatcga caagcagagc tagaagcagc aatggaagat
    39361 gaaatgaaag caaatggcta cgatctgttc gtattagttg tcacaaacat cttagacagt
    39421 gattcagaat tacttgttgc tggtaaagga aaagaaaaag tagaagaagc tttccaaaca
    39481 actttgacca acaaccgtgc tttgcttaaa ggtgtcgttt cacgtaaaaa acaagtcgta
    39541 cctcaattga cggctgtatt tagctaatca aatggtagta tgaagcattc aaaatgaatg
    39601 gataagcgat tgatataaaa agaaacatag aacatactaa aggccgaaga aatggaggag
    39661 aataacagtc aattatgact cttgttcttc ctccttttta cgtaaaagcc ttttaagtaa
    39721 aataaaacgg atagaaaaga gaacatatat gaactattat tcaaaaaata aggaaaactt
    39781 ttatcaaaaa ctgaccgatg atccgctttt ttcttcattg accgattatc tatatgaaca
    39841 tcgagaacaa gaaacgattc taagagaatt gaaaaaagaa ttttcgcaaa ataaattttc
    39901 ccattttctt gatttattga tcgatgcagg attaatcaag agggaagaac gaagatatca
    39961 tttgaacttt ccaatatttg atcctaaaga ttacttgcaa caagccactt cggctgctga
    40021 aaccatagct gaacaattga agagactctc tgttgatgaa caaaaattag cgatgggaga
    40081 agttatttgg gcgtattgtt tcgaagatga acggaaagaa gcgtattttt atggagtaag
    40141 aaacagccga gagacagaat tgctgaggac aactgcagga aatgagaagt accgttttat
    40201 cacactttcc tctatagaac acttcccgct tactttggct aattactttt ttgtacagaa
    40261 gaatcagctt cctgtaacga aggcattcaa agaattagcc gaacttattg gagacgtaaa
    40321 tgaatcgtac ttttttgatc aaatagaagt tatcgttgac aggatacgta agaataaata
    40381 taaaaatcgc cggcctagta ttttccatca gtctctactt gtgacgaata ctataaagga
    40441 agaagaatca ttcacattag aattacctat tgtcgaaaaa aacaattttg agattgaatt
    40501 accaacattg gatccttcat tgacaatgga ggaaatggcc tttcttaaac gacaaatctt
    40561 ttctgagctg agcaagaaat ttataccgca tgcattttct tatatcaaag aatatggaac
    40621 agttttagtg agtaaaacct aagagagcag taagcaaaaa ttatactgac tcttgcagga
    40681 ggagcctttc tcttccttta tggttaaaag aattatttga gtattttgcc tatctaccac
    40741 ctattagatt cactacatgg tctggaaaca aaggaatgca aaaaaatggt gagaaatgtg
    40801 gaatgacaaa ggaagtagtg ataagaaaag ttagattttt aaataatcaa tattatgatt
    40861 cagtcaagta tggcatttta tgggaagaac tagctgatta gattaggaag tccatttgat
    40921 aatccagaat cactaagaaa cgaatggcga gtcattaata atttatccaa tcttgggtta
    40981 ttcttttaga ttgaagtgtt taaaactaaa aaagcattta tgaagtcgta atagtcaggc
    41041 ttcaaaatgc tttttatttt gtttgttttt tctttgccac ataaatacac ctagactgac
    41101 gatgaagagc atatccaaca gatttcctgc gacttggatc gtcgaagggt tggtcagtgg
    41161 cattaaagca aggacaaaaa aggaaaacaa gctgtaaata aaacaggtga caggaactaa
    41221 tcgaaagaag agcggacatt tcaattggct aaaaatgagt gaaaaaataa aataagtaaa
    41281 aatggataaa atcacgacta aaccattcat gataaagaag aaaagcgtat tgtgattaaa
    41341 cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat gcaatcaata cgcttgaagc
    41401 ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca ccgaaataaa cagaaaacag
    41461 taagccgcaa agagaagaca aaatcaaaaa aaacggattt tcttcggctt ctgtgatatc
    41521 agaatgatcc aaaagcaacc agcctaatgc actagaaggc gcatgtgagg tcttaaggta
    41581 tgaataacat agaaaaaata agatcgtcaa agttgcaaaa aaagagccta aatgtaaaaa
    41641 attagtggtt ctacgttgaa tttccataat atctccaaat taaattgata agtatataaa
    41701 gtataataac aaattgatga gtatatgtcg atgtttttta atcaaaataa taaataaaaa
    41761 ctgtctaaca tttaaaattt aatttagata ttagacagtt tttaagtttg tgagttattt
    41821 tccttgtcgt tttctcactt ttttagtcca gaaaccacct gaaatatatt ttggctcata
    41881 agaaatgata aaagcacgct cttcgatttc tttgatcagg cgatacagtt cacgttcgct
    41941 ttttctagga gaaagtattt cgagaactag ccgctcacct tccagcccca ttgcagaagt
    42001 ctgtgtcaca ccataaccgt gttctctcag tatacccggc aaacttttat cttctgcgct
    42061 atttgctggc aaaatcgttg tgaccatgat gtatcctaat gccaatttgt cttcaatcaa
    42121 aattccgaca ctgataccta ctgcgtaacc taacgcatag acaaataaat tcagtggatt
    42181 atctaaacga tctaaaacaa gggataagcc gacgatataa atcgtgatct cgaccatact
    42241 gacaagtgga gcaatcagtc gatagccttt cattgtcaac atgaaacgta acgtgttcag
    42301 agtgatataa gcaaaattaa tgaggaagat catccccaac atttttggat caatcatttg
    42361 taaaactcct ttcatttata taacaacaat atcaaaaaaa atcgtaagtg aagaaaaatt
    42421 gaaactttat acatacaaaa aaatgataaa atgattataa tagaattata aagataggtt
    42481 aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc cagccacgtg ctaagtctcc
    42541 ttctcagtat atacttaggt gtctattcga ttcaacagcc aattgaggta gagaacattg
    42601 tttttcagat gtcgctgaca gatggtttaa ttttaagcgt ttttttcttt ttgatactgt
    42661 ttattggtaa tattttcggt agcgttgcca gttttttgaa tttttctatt tatccactcc
    42721 tttcgctagc attaggagtg gtgggacttt gttctctgtg cttatttccc gagccattca
    42781 gtcctgcatt tatattattt ggtacactaa acctgttcca gacaactgtt ggagcatggt
    42841 tgctatggcg atcaggaaat ttaatgaaaa taggtgagta acaattgaat ccaattatat
    42901 tgaaacgttc tgtccatcat attccagtgt tagaagttgt tctgaaagaa ttaagagagg
    42961 aacgtattcc cgttgtcatc tattatcacg gatggcagac aagtaaggag ttagttttga
    43021 cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat cctgccagat gcgatgaatc
    43081 atggggagcg caaacagccg gtttcttcta ttccatccta tacattttgg gatagtatct
    43141 acggaaatct gtttgaattt gacacactta tcaagcattt agaaaaaaga ggtcttctac
    43201 atgatcagct tgcagttggc ggggtttcga tggggggaat gacaacatgc gccttgttgg
    43261 ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg ttcccctgca cctttggaat
    43321 atggaaaaga aatcgtaagg cgcgcatcgg aatataatta tgtcgtacca aaagactatt
    43381 ttgatttgat ttcatgggtt aagagctatg atttgtcatt gcagccggaa aagttagctg
    43441 gacgtcctct gttcttttgg catggacaag ctgacgaaaa aatcccttat atacaaacgg
    43501 aacgatttgt agaaaaaaat cggcataagt cttacggaaa agaaattgtt tttcgttcct
    43561 caaaaacaca gcgacatatg gtacaaatac cactaatgga agaagcttcc acttttttag
    43621 caaaaaattt gataaaaaag tataaataat tgaaacggaa aatcactcat gacgatagag
    43681 gattttccgt tttttatttt gggctataat aaactctgtt aggaaaattt ggtataattg
    43741 atagtaagaa tgacaacatg ttagaaagga agagagcatg cccactttag cagatgttgc
    43801 aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc atcaatcatc cggaacaggt
    43861 gactgacgaa ttgaaagagt tagttttctc agcaatggct gaactggatt accgtccaaa
    43921 tattgctgca aaagcacttg tcacgaatcg gtcgcagatc atcaaattat ttatactgga
    43981 agaaatcgac acaactgagc cctattacat gaatttgctg atgggaatcg ctaaacggat
    44041 cggtaaagcg cattattctc tccaattagt tacaaatgat gccttcgacg tcggttcttg
    44101 tgatggatac atcatcacag gtgtcagaaa aaaagacttc gattggatcg accgtttgga
    44161 aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat gattatgtag acagcgataa
    44221 tgaatatggt acagcaaaag caacgcaata tgccttagaa agagattatc aaacgattat
    44281 ttatatcggt atcgaagtgg acgagccttt tgaaatttca agggaaactg gttatttatc
    44341 tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg tttccaaatc attcgacaga
    44401 agtagaaaga tatatgacag aaaactgggc agattttaca gataaaactt gttttgtatg
    44461 tagttcagat cggctagcac taggagcagt aagagggatt ataaatgctg gaggacactt
    44521 gcccgaagaa tttggtgtga ttggatttga tggggtattt ttagaccaaa tcagttcacc
    44581 taaattgaca acgatcaagc aagatattat acgtatgggt gaagtatgtg gagaaatgtt
    44641 gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat cgtcgttttt ttcctgaact
    44701 agttatcaga gagacaacga attaagtctt attactatat atatatttat gaaattcatc
    44761 gctattttct taaaataatc agcgcattgc tactgaaaac aatgaatctg tgctattatt
    44821 gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa gaaatcagac gaaaacattt
    44881 ccgttttcct gcgtacgatg atgagttagg cgtaaaatta accaacgaca agaagcgagt
    44941 attatttgac ggacaagaag atcttttgac tgaacagaat cagtccccgt ttgaacagat
    45001 gaaagaattt catttcacga acacgaacac cgagaaaatc cgtcgcaagc gtcgtgcaac
    45061 aaatggattg aagtcagctc cgcgtaagga aaatttggaa aagcataaag gaaaattacc
    45121 ggattatacc ggccattacc aatcagaaat cacatcgaca ggacgaaaaa aactttttgg
    45181 tgattcctct cgttctgtca gacaaagtga aacaaggaca gagtccaagc atactacacg
    45241 aaacgcatct gttgacacga aggtgcggga gcgttcttat tttgttccta aatatgtacc
    45301 cgcatcgatt atcccagacc ctagagaaag caaaatttct gaggatcatt taatcgaatc
    45361 gatgaaaaaa acggatgact cttatctgtt gttcgataca gaaccgacgc cgtatcaaga
    45421 aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga acaaaaacgt tagaggaaac
    45481 aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt atcttagaac gctcattaca
    45541 aggattgatc gatgatcaag gaaactcttt gggagaaaat agctatttta aataagtgat
    45601 atacaaattt atattcggct acagttggaa aggaaacgct gcggcgtttc cttattttat
    45661 actctatccg aaagtttata tcgtatcctc aaattttttt gagtttaagc gaattttttc
    45721 tcccaataag taagaaaata atggcaaaat aaaaaaacaa actattttcg cagatcgttt
    45781 gcaaaaatag tttatttttt tataaagaag attttactga ataatatcat tttagtttac
    45841 ataatgatat ttctgtaaat taaataaagt agtgaaaaat aagaatcagt gctctaactg
    45901 ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt tttgcttcca attggggcaa
    45961 aacagatcca atttccagat acttcacttt attcaatgta taagatagaa tagaacagct
    46021 aagtaatgta tacagaaaat gtgtgagatc caaataaagc aaggagacaa ctaaaatacc
    46081 aaaagtaatc aagaaaagac ttttgccaat cttaatatga aaatattttt caatggcttg
    46141 ataaatgata tctgttccgc ctgtgcttcc gccgaatcga aagacgagac cgcaaccaat
    46201 gccagaaaga atacctgtca tcaaactatt gatcagtaga ctatctacag caaaatgtat
    46261 atgaaggctc tcccataaat gtaaaaagaa aactagcgca aatgttccga cgatcgtccg
    46321 gagaaaagcg tgttttccaa aaagaaacaa tgagataagc aataaaggta aattaaaaag
    46381 cagtgaagtg acagaaggag cgaatccaaa aagattatgc aaaagcaaag caataccagt
    46441 aaccccacca tctgccagct tagatggaat attgattcct gtcacagcta atccgtagat
    46501 tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa tttaattttt tcatttctag
    46561 cctcttttct tttgaaaatt ttcagcaact tctttattat actgacttat actcttactt
    46621 caagaggaaa atgaaaaaaa attatttttg gctgtttctt tttatttatc aaataaggag
    46681 tggaaaaatg cgagcagcag acttattgac aattcttaca aaatcaccca aaaaaacaga
    46741 gtatcaagga gtttttttgg ctcaagaaca aaaactggtg cctgtgacaa tggtccagat
    46801 aaataaagca aatcaattta tctttttatt tgaacacggc aaagcaccac ttgctatgaa
    46861 agacatcttt ctttttttga tgaagaaccg tgagaaagct gtttattatc agtcaaaaga
    46921 agaactcatt cctctttatg gagtgaaaga gatagaaaat aaagtggtga tataagtaaa
    46981 aataagaaaa caagacgttt atgatgtcaa aacaaccatt ttttacattt ttggcaaaaa
    47041 agtgtcatga tacagtataa tattcgttga acaggccaat aaaggtgttc tcccccacga
    47101 gatttctact ttattggctg tttcttttta ttaaaataaa agttgtgtgt gtaaaaaaga
    47161 tatagctgat tccaagtgac aatttcgtat agatttttat ggtattcgta gtgaaaaaac
    47221 gagaaccaaa aagttgatcc gactttgttg cttggattct ttttttgtca gcatatttag
    47281 aggtattttt ttcttttttt tgttcgtgct gtaaggttaa ggcaagacaa gttagttggg
    47341 aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa ggaaggtcgc atgaataatt
    47401 ttatacaaac attaagtgaa agaaaaggcg agttgctgga agctgcattt caacatctgt
    47461 ccatttctct catttctttg ctgattgcag cactgattgc tattccttta gcgatttggg
    47521 cagccaatca taaaaaaatg gcagaagtct tattgcagat cacgggtgtg ctccaaacga
    47581 ttccttcact tgccttatta ggtttactta tcccatttgt agggatcgga actgttccag
    47641 cattgattgc acttgttatt tacgctttgc ttccgatttt ccaaaacacg tatattggtc
    47701 tagctgaaat tgacccgtct attgaagaag cagcggtagc atttggtatg tccagaatga
    47761 gacgcttgct gaaagtagag cttcccatcg cattacctgt cattatctca ggtatccgaa
    47821 ctgccttagt tttaatcatc ggtacggcta ctttggcagc attgatcgga gcaggtggat
    47881 tagggacatt tattttgtta ggtattgacc gaaatacacc tgttttgaca ttgattggtg
    47941 cgatcagttc tgccttgctt gcaattatat tcagcggact gattcgctgg ttgcagcata
    48001 agaaacctcg atattccatc attactttag cagcagtggt gtgtttattt ggcggcgttt
    48061 ctttatatca gtctgccttg tttaaaaatg aaaccatcac gattgctgga aaattaggag
    48121 cggaacctga aatcctgatc caaatgtata aagaattgat tgaagacaac acaaaaacag
    48181 aagttgaatt aaaaccgaat tttggcaaga cgagtttttt gttcaatgca ctggatcaag
    48241 gtcaaatcga tatctatcct gagttcacag gtactgtcct agaaagcttg gtgaaggtac
    48301 ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac atatgacgaa gcagaagaat
    48361 tattgaataa gcaattcgat atgacattat taaaaccaat ggcttaccaa aatacgtatg
    48421 ccttggcagt caagagatct tttgcgaacg aacatcaact taaaaagatt tccgatttgg
    48481 cagctatctc tgatcagatc aaagcgggat tcacactgga atttattgat cgttcagatg
    48541 gatataaagg aatccaagaa gcatatggct tgaatttttc aagtgtacaa agcatggagc
    48601 caagtttgcg ttaccaagca atcaataatg gagatgtcaa tattgtggat gcctattcca
    48661 cagatagcca gctcaaggag tacgatctag tgactctaga agatgatcga gggttattcc
    48721 cagcttatca aggcgcaccg ctgatgaaaa aagattttgc taaagaacat cctgaaatcg
    48781 ttaaagcact gaataaatta agcggaaaaa taacagagga tcaaatgatt gatatgaatt
    48841 accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc acgaatcttc ctgaaaaatg
    48901 aaggattagt aaaggaggaa gatcagtgac taaattaatc gagttcaaac atgtgcaaaa
    48961 aaagtatgat ggaaagtatg tcatcgatga cctaaatctg gcaatcaaaa aaggagaaat
    49021 ctttgttttg gttggtcctt ccggtagtgg aaaaacaact actttgaaaa tgatcaatgg
    49081 attatctaaa ccatcagcag gagacatcta cttcaaagga aaatcgttaa atgaatataa
    49141 tctgcagaaa atgcggtgga atatgggata tgtgctgcag caaatcgctt tgttcccgac
    49201 catgacagtc aagcaaaata ttgaagtcat ccctgaaatg ttaggttggg aaaagcagaa
    49261 aagagcagac cgtgtagatg aacttcttca aaaagttggc ttatctcctg atatttatcg
    49321 agatcgtatg cctagagagt tgtctggagg agaacagcaa cggatcggga tcatccgagc
    49381 tattgctgca agcccagatg tgattttgat ggacgagcca tttagcgcat tagatccgat
    49441 ttcaagaaac tcattacaag aattggtttt gtccttacac gaagaattgg gcacgaccat
    49501 cgttttcgtt acgcacaata tggaagaagc aatcaagcta ggtgatcgta ttgcatttat
    49561 gaaggatgga gaaattatcc aatgtgacac accagagcag ttattgatga atcctaaaaa
    49621 cgactacgtt cggcatttct ttgatgaacc gaaacagacg aaagagtggc gagtagaaga
    49681 tttagtcgtc aatggatatt tcttaaatga aataccagaa aatgctcgtc aacaagtttg
    49741 ctttgatacg cctatgaaag aagtatattc gcttctttcg gtgtatccaa gcattgtgat
    49801 agtagaaaag caaagaagta ttgggtcgct gacaagtaaa gaaatctttg cttttttaag
    49861 tcgagaggag gaaggaaatg aaaacatatg atgcaatcat catcggttca ggtgtgagcg
    49921 gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac agtactagtg gtcgaagaag
    49981 atctatgggg cgggacttgt cctaaccgtg gatgtgatcc taaaaaagtc ttgttaagtg
    50041 cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa aggttttaac gagatcccaa
    50101 ctgccaactg ggaagaactt caaaaattca aacgaacatt tacggatcca gtcccagaaa
    50161 gccgaaaaaa acaattagca gaagctgaaa tcgatcatct ttcaggaacc gctcgttttt
    50221 tggatgattc atctattgaa gtcaacgaag aagtcttcca tgcggattac ttagttttgg
    50281 caaccgggca aagacctacg atcttaccag tggagggaaa ggaatacttg aaaactagtg
    50341 cagacttttt atcattgcct gtactgccta aagaaatcat ttttatcggt ggtggttata
    50401 ttgcttttga gctagctacg attgccaatg ctgcagggag caaagtaacc attgtccatc
    50461 ataatcaacg gccattaaaa gaatttgagg cgtctttggt agaggaagcc gtccatcaaa
    50521 tggaagcatc tggcattcag tttgcttttg gtgttgaaac ccaaaagatc atttcagaag
    50581 gtactcgcta tcgtttagtc ggaaaagaga cggaattagt agcagatatg attttttgtg
    50641 cgactgggag acagccgaat acagaatctt tagcacttga gcaagcgaat atcgtctttg
    50701 ataaacacgg aattgcagtc aatgattatc tgcaaacgag caatccaaaa atttttgcgt
    50761 gtggagatat tgtttcaagg aaaaccccaa aattgacacc agtcgccact tttgaaggaa
    50821 attatgtagc taagcggata acggatgcaa caagcgaacc tatcaaatat ccgattatac
    50881 ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg agttacaaaa agtcacgcct
    50941 cttcgtcaga tcaagtggta gagatggatc ttaccagttg gttcacctat catcgtgtca
    51001 atgaaccagt tgctaaggct gaattgacct ttgaccagca aaattatttg attggtgctg
    51061 cagtgattag cgagcaagca gacgaattga tcgatgattt gacactcgtc atcaatcaaa
    51121 aattgactaa aaaagagttg gatagctata tcatggggta tccgacattg gcaagtgatc
    51181 tctcctattt attgaaataa tcagcatgat aactaaagcg cttgttgctt tttccttctt
    51241 gtgctacact ttaaaaaatg aatacctgtt tctggggatg tcgtagacga catgagagta
    51301 agttgtgaac ttagacccat tgaacctgtc agttaatact ggtgaaggga gaaacatagc
    51361 agacgcttga agcgggttac tatgtcgtgc ccatttcaag cgttttttgt tattcatatt
    51421 agaagaaagg aggtaacaaa atgaagaaaa atctttatcc agcagtcagt atggcattca
    51481 ttttaattat ttggcagatt gttgtcatag tttggaaaat acctgccttt attttacccg
    51541 gacctgctgc tgtattttct gcattagcgg cagatgcaca agtattactt tcccattctg
    51601 tcacgacatt aaatgaagca gtgcttggtt tggtaatagc tagcgttctt tcttttatta
    51661 ctgccttggc aatggattat tggaagtttt tagaaatgtc tttttatccg ttactagtga
    51721 tttcacaaat gttgccaatc atggtgttag ggccattact taccctatgg tttggtttcg
    51781 gtatgcttcc aaaagtgatc cttgttgtct tgataagcta ttttccgatc gtagtttctt
    51841 tttcggatac attgaggaaa gtatcaaaag agcaaatcat ctttttgaaa acgatggggg
    51901 cagatacagg gaagatttat cgaatatata aaataccagc gggtttatcc ggcttctttt
    51961 ccggtttaaa agtggctgct acttattgtg tcagcggagc agtcgttgga gaatggctaa
    52021 gcgctcaagc aggcttaggg tattacatga tacgcgtaaa aaacagctac cagatcgata
    52081 aagtttttgc tgcgatcatt tgtgtcattc tattgagttt attgctgaat ggcgcttgtt
    52141 cattattgaa aaaagggtat tatcgtatgc tttatatttt ttaagagaag gggatcaaat
    52201 gaaaaaatta tggcttttat taccgctact gttgctttta tccgcttgtg gcactgcaaa
    52261 agaaacaagt agcaaacaag aaataaagga tttgaaaaaa gtgacacttg ttttggatta
    52321 tgtaccgaat actaatcata ctggcatcta cctagcaaaa gaaaaaggct attataaaga
    52381 ggctggttta aatgtccaaa tcatcgaacc aggagataat tcgacaagta ttggtctagt
    52441 aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa gatgtcactt atgctcacgc
    52501 agatggacag aatataccag tcaaagcaat tgctacagtg atcaaacata atacttctgg
    52561 attcgccacc ctatcagaca gtaacatcca ttcaccaaaa gattttgaag gaaaaacata
    52621 tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa gctgtgatgg aaaaagacgg
    52681 cggggacttt tcaaaactga caatggtagg aagcaatgga gaagggccag aaagcttagg
    52741 aaaatcctca gatatccaat ggtactttga aggttgggat atgatcaaag caaaagaagc
    52801 gggtattgaa ctaaactata tcccattgaa agaattagat gaacggttag attactatac
    52861 tccggtaatt attacgaatg atcaactgat caaaagtgat ccagaattgg tacagtcttt
    52921 tatggatgcc acgaaaaaag gctatcagga agctattaag gatccaaatg acagtgctaa
    52981 actgttgcaa aaatatgcaa aagaaaatga tcggacgttt ttagaagaat cacaagcatt
    53041 cttgtcaaaa aattatacgg atgatcctga gaattgggga ttgatggaag aaaaagtttg
    53101 gcgcaattat acagctttta tgcaggaaaa cggattgatc aagcaagatg taccttcgca
    53161 agaactattc actaatcagt ttataaaata ggagtgatag atatgtcaat ccaaatcagc
    53221 cacttgaatg tagtcattga aaacctgaaa atattagaag acatccattt cactatccct
    53281 aaagatacct ttacatcgat tgtcgcgcca agcggagccg gtaagtcaac attgctgaaa
    53341 actttaacag gagtccagcc tatgacttct ggaacgatca aagtagacga tcaaaaagta
    53401 actggtctcc atacagcatt tagttatatg ccgcaagaag atatgttact tccttggctg
    53461 agcgtctatc aaaacgttac gttatatcaa aaaatcaatc atctaacaat caatgaagaa
    53521 caagtaaggg attatctgga tatctttggg ttaaacggct atgaacattt tttgccggaa
    53581 caattatctg gcgggatgaa gcagcgaacg gccttgttac gaacaatcat gaatccttct
    53641 tcttacttgc ttcttgatga accatttgga gcgttagatg ctatgaccag agggcagatg
    53701 caagactgga tgttgaagct gcctaaaaaa gcaaagcgga ctactttact tgtgacgcat
    53761 gatatcgaag aagcaattta tttatcagac aggattcttg tgctttccgc ccgtccggca
    53821 catgtgattg ctgagattca agtgcctgaa aaacgacggt cacgtgaatg gctactgcag
    53881 caatcagagt tgaagcaaac gatttatcag ttattggcag gtgaattaga tgttaagtga
    53941 tgctcattgt catttagatg ggagtcagag tttagcactc cttcagcagg aacacacgat
    54001 tttgacgatc atcaattgtg attcgccaga agaatggaag gaaaacagac agcttgcagc
    54061 aagtaagact caagccttaa gttacggtat ccatccttgg aaagcagata gctatacgtt
    54121 tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc ggtgaaatag ggttagataa
    54181 tatttggacg aatgtaccga tgactacaca aaaaaaagta tttgagcgac agcttgcttt
    54241 tgcagccata aatgagaaac ctgttgtact gcacacaaaa ggatgcgaaa aagagatctt
    54301 aaattatatc caagcttacc ctaatcacta tttgatccat tggtattctt ctttggacta
    54361 tcaacaagac tatattgatt taggctgtta tttcagcatt ggtcttgatt tgaaaaagaa
    54421 tccagcagtc tggcaactag ctaaagcagt cccaatcgac cacttactta ttgaaacgga
    54481 tggactacat gcagcagagt gggctttagg ggggattttt tcagaaaaag attatgtctc
    54541 actgcttgaa gaacagttaa agacaatcgc tgagatcaaa gagctatcag ttgaagaagt
    54601 taaaaaaaat acttatcaaa acttggaacg tttcattaga ttaggtaaat aaaaaagaga
    54661 tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt gtataaaagt aaactgtttg
    54721 attaat
//
LOCUS       DACDYM010000009        53131 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDYM010000009 DACDYM010000000
VERSION     DACDYM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.009"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(369..995)
                     /locus_tag="IWM59_000568"
     CDS             complement(369..995)
                     /locus_tag="IWM59_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978626.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="IWM59_000569"
     CDS             complement(1290..1625)
                     /locus_tag="IWM59_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9978627.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="IWM59_000570"
     CDS             complement(1612..1896)
                     /locus_tag="IWM59_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9978628.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="IWM59_000571"
     CDS             complement(1952..3475)
                     /locus_tag="IWM59_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9978629.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="IWM59_000572"
     CDS             complement(3468..4643)
                     /locus_tag="IWM59_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9978630.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="IWM59_000573"
     CDS             complement(4647..4832)
                     /locus_tag="IWM59_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978631.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="IWM59_000574"
     CDS             complement(4798..6492)
                     /locus_tag="IWM59_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9978632.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="IWM59_000575"
     CDS             complement(6489..6962)
                     /locus_tag="IWM59_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9978633.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="IWM59_000576"
     CDS             complement(7031..7186)
                     /locus_tag="IWM59_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9978634.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="IWM59_000577"
     CDS             complement(7320..7700)
                     /locus_tag="IWM59_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9978635.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="IWM59_000578"
     CDS             complement(7704..7919)
                     /locus_tag="IWM59_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978636.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="IWM59_000579"
     CDS             complement(7922..8329)
                     /locus_tag="IWM59_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9978637.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="IWM59_000580"
     CDS             complement(8595..10070)
                     /locus_tag="IWM59_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9978638.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="IWM59_000581"
     CDS             complement(10060..10908)
                     /locus_tag="IWM59_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9978639.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="IWM59_000582"
     CDS             complement(10945..11307)
                     /locus_tag="IWM59_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978640.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11607..12231)
                     /locus_tag="IWM59_000583"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="IWM59_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="IWM59_000584"
     CDS             complement(12228..12539)
                     /locus_tag="IWM59_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978641.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="IWM59_000585"
     CDS             complement(12583..12876)
                     /locus_tag="IWM59_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978642.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..13717
                     /locus_tag="IWM59_000586"
     CDS             13070..13717
                     /locus_tag="IWM59_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9978643.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13778..14923
                     /locus_tag="IWM59_000587"
     CDS             13778..14923
                     /locus_tag="IWM59_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9978644.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15362..16180)
                     /locus_tag="IWM59_000588"
     CDS             complement(15362..16180)
                     /locus_tag="IWM59_000588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978645.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(16280..17605)
                     /locus_tag="IWM59_000589"
     CDS             complement(16280..17605)
                     /locus_tag="IWM59_000589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978646.1"
                     /translation="MENIEETFLKVLSPYISDSILRFQEQEKNYKFGFDIKNYNDSLH
                     VVFQYNKTGKIGIKEVHIFQIFLAKVNIEASKSKLNYTTLLDTNALFFSKKLLPYLHS
                     YEWALRKLIYLLAPAYFSDNWAEESIPNEIISSIKRKQIQAIGKFDSKNLLQWIDLYD
                     FEEYLFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYSLWSEVFSKYM
                     DVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEKAFQKILAGDI
                     AGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKKSMESIGQTLS
                     VQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLSALSAQRAIMQ
                     NFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKSE"
     gene            complement(17774..18703)
                     /locus_tag="IWM59_000590"
     CDS             complement(17774..18703)
                     /locus_tag="IWM59_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978647.1"
                     /translation="MRSDSRFFVSNLQDDELIRQIDSLLETITESDKRIFLNYVELTR
                     HIIELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGEKLTEDQYYYQINALTINLISSA
                     KTLTESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLLHMRNYSQHGHLPVNIH
                     QQRVYFDLDEILAMPHFDFNGQLKKELDVLRKDIYNQFRDSPRISYVYTVAKFNLVIT
                     EIYFNYLKEIKPILIKLNDEKNELLLNTKFQLINSDGSSSDMLFYEFDGENYHCFNRK
                     DNSISMYADIKREVKKILKVEEQYYKEIEKRNQ"
     gene            complement(18951..19301)
                     /locus_tag="IWM59_000591"
     CDS             complement(18951..19301)
                     /locus_tag="IWM59_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978648.1"
                     /translation="MIFENESEEIGRMAEENKVVPFETLVINKEEFEQLFLDFFIQYD
                     RIYDFSSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFR
                     EFRNQIIRSSNIEN"
     gene            complement(19342..19836)
                     /locus_tag="IWM59_000592"
     CDS             complement(19342..19836)
                     /locus_tag="IWM59_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978649.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
     gene            complement(19829..20143)
                     /locus_tag="IWM59_000593"
     CDS             complement(19829..20143)
                     /locus_tag="IWM59_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978650.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(20359..21750)
                     /locus_tag="IWM59_000594"
     CDS             complement(20359..21750)
                     /locus_tag="IWM59_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9978651.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(21735..22346)
                     /gene="mobC"
                     /locus_tag="IWM59_000595"
     CDS             complement(21735..22346)
                     /gene="mobC"
                     /locus_tag="IWM59_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP9978652.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(22350..22547)
                     /locus_tag="IWM59_000596"
     CDS             complement(22350..22547)
                     /locus_tag="IWM59_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978653.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNEIESDFKCCSLEKNVRG"
     gene            complement(22561..25254)
                     /locus_tag="IWM59_000597"
     CDS             complement(22561..25254)
                     /locus_tag="IWM59_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HAP9978654.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADSIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAK
                     NSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQG
                     TKTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDV
                     RENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIID
                     SEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            complement(25535..26548)
                     /locus_tag="IWM59_000598"
     CDS             complement(25535..26548)
                     /locus_tag="IWM59_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9978655.1"
                     /translation="MEEIDKLIAQYAYSKDDVYAEKYLKYPIFLDKHKKYKKMKQSTK
                     KVYMYLKKQNEYSLENSKVDSKGRIFLIFAIEKIAEKSCLTKKTVISALRELEEYKLI
                     TIKKNGFNKKTGKNNPNFYYLLKPEFSEGDFYKKKCIEENLHLPTNQHYSPSEKNTPS
                     DKSTFDGDKSFPLPGEKFLQELNHSSKDFKDFKESQNADFIDSLSVENKKLDEILIES
                     LIEKEWLYQVWGDNIVNCLKLVGNNDYSQFYDWYKKFKYAIKSAEKEQKIQLKIIYEI
                     CNNKYFVDYVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGI
                     DKQ"
     gene            complement(26752..27033)
                     /locus_tag="IWM59_000599"
     CDS             complement(26752..27033)
                     /locus_tag="IWM59_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9978656.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
     gene            complement(27084..27290)
                     /locus_tag="IWM59_000600"
     CDS             complement(27084..27290)
                     /locus_tag="IWM59_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9978657.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            27454..28185
                     /locus_tag="IWM59_000601"
     CDS             27454..28185
                     /locus_tag="IWM59_000601"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9978658.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLFLAVSKTYSLDKSPSLKDNFVEMIIDDVYKKLLQTDKSDDQLILG
                     FIYICLDEKLSNRQKSLKSINYEVFNYLGVAFGLIDSEIKSTNAGNDEDLLNYLKELT
                     SFIDEIKTTTFNKKKYFQNQYDIEE"
     gene            28239..29384
                     /locus_tag="IWM59_000602"
     CDS             28239..29384
                     /locus_tag="IWM59_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9978659.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(29469..29774)
                     /locus_tag="IWM59_000603"
     CDS             complement(29469..29774)
                     /locus_tag="IWM59_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9978660.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(29799..30230)
                     /gene="ruvX"
                     /locus_tag="IWM59_000604"
     CDS             complement(29799..30230)
                     /gene="ruvX"
                     /locus_tag="IWM59_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9978661.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(30227..30493)
                     /locus_tag="IWM59_000605"
     CDS             complement(30227..30493)
                     /locus_tag="IWM59_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9978662.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(30748..31473)
                     /locus_tag="IWM59_000606"
     CDS             complement(30748..31473)
                     /locus_tag="IWM59_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9978663.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(31834..31904)
                     /locus_tag="IWM59_000607"
     tRNA            complement(31834..31904)
                     /locus_tag="IWM59_000607"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31870..31872),aa:Gly,seq:ccc)
     gene            32021..32773
                     /locus_tag="IWM59_000608"
     CDS             32021..32773
                     /locus_tag="IWM59_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9978664.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            32789..33268
                     /locus_tag="IWM59_000609"
     CDS             32789..33268
                     /locus_tag="IWM59_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9978665.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(33519..35099)
                     /locus_tag="IWM59_000610"
     CDS             complement(33519..35099)
                     /locus_tag="IWM59_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9978666.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(35092..35835)
                     /locus_tag="IWM59_000611"
     CDS             complement(35092..35835)
                     /locus_tag="IWM59_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978667.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(36011..36787)
                     /locus_tag="IWM59_000612"
     CDS             complement(36011..36787)
                     /locus_tag="IWM59_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9978668.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(37035..37871)
                     /locus_tag="IWM59_000613"
     CDS             complement(37035..37871)
                     /locus_tag="IWM59_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9978669.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(37882..38811)
                     /locus_tag="IWM59_000614"
     CDS             complement(37882..38811)
                     /locus_tag="IWM59_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9978670.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     CVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            39003..39356
                     /locus_tag="IWM59_000615"
     CDS             39003..39356
                     /locus_tag="IWM59_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9978671.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(39430..40086)
                     /locus_tag="IWM59_000616"
     CDS             complement(39430..40086)
                     /locus_tag="IWM59_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9978672.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            40312..42246
                     /locus_tag="IWM59_000617"
     CDS             40312..42246
                     /locus_tag="IWM59_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9978673.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(42332..43555)
                     /locus_tag="IWM59_000618"
     CDS             complement(42332..43555)
                     /locus_tag="IWM59_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9978674.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(43549..45447)
                     /locus_tag="IWM59_000619"
     CDS             complement(43549..45447)
                     /locus_tag="IWM59_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9978675.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(45441..46559)
                     /locus_tag="IWM59_000620"
     CDS             complement(45441..46559)
                     /locus_tag="IWM59_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9978676.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(46642..47475)
                     /locus_tag="IWM59_000621"
     CDS             complement(46642..47475)
                     /locus_tag="IWM59_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9978677.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(47468..48385)
                     /locus_tag="IWM59_000622"
     CDS             complement(47468..48385)
                     /locus_tag="IWM59_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9978678.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(48612..49736)
                     /locus_tag="IWM59_000623"
     CDS             complement(48612..49736)
                     /locus_tag="IWM59_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9978679.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            49919..50827
                     /locus_tag="IWM59_000624"
     CDS             49919..50827
                     /locus_tag="IWM59_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9978680.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            50968..52581
                     /locus_tag="IWM59_000625"
     CDS             50968..52581
                     /locus_tag="IWM59_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9978681.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        16204 a        10379 c         8244 g        18304 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13561 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13621 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13681 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13741 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13801 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13861 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13921 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13981 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    14041 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    14101 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    14161 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    14221 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    14281 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    14341 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    14401 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14461 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14521 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14581 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14641 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14701 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14761 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14821 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14881 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14941 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat
    15001 agtctattct tcttcttccg ccatgaagtt aagctcatta aagataattg acgatagttg
    15061 gtatttcaac gatttttgaa tagaactaat cactttatta agtctaattc accttaagtg
    15121 gttttttagt cgtattcaaa aacgtattca agttgcgtgg cgtgcttttg ataagaaatc
    15181 gtaagaacta gttaatcaaa cgacttataa ataatgtaac accactgatt aatcagctat
    15241 atccaaaaaa acaagcattc attcagatac atcaatctct atagaataca aagctttcat
    15301 tatctcaaac cattaccaca acttggcaag tcggttaatt gaagaacacc tcattctcca
    15361 cttatagcag tggagcaaca agttgagcta ttgttgcaac agaagctaag aattctgtac
    15421 tccatttaag tttttttaga ctatctaccg caaacctcag catagttttt ttaggctttt
    15481 ctttaatagc ttcatcttta atggtttcga tatactcctg agcctcttct acctcttctt
    15541 caggtaagga atcattcaaa ttctccaaaa gcaaatcaag ctttgataat aattccgaca
    15601 tatcatcaat agaaacaatc atattattat tgtggccttg ctgtatatta gtgttgtaac
    15661 tatttccaaa tatatttact ttattatata ttgaattgtt gctcatatag tcctccagtc
    15721 gtcctgctgc attacttgca tcttgtaacg tcacaaaaaa atctctccca tgttggtaaa
    15781 ttgattcaac gatttcaaat tctggatttc catattcgcg ccattcgtat tcttccttaa
    15841 agtattttcc gtaactatgg ttaaattgca cagaattttt accaattttc cttagaagtt
    15901 ttatctctct ctcagaaata aaattttcct caaaagctga ttcaactatc tgaaaatcac
    15961 taagaaatgt tcctaaacag aagattgatt cattaatagt ttgttgaaaa ttccatggta
    16021 ttttgttcca aaaaccattc ttaattaaag cctctttttt ttcaacaatc tgtgatttta
    16081 cattttctgt atatatcttc caattattga cgtcttcctt aatgtccgaa atcgattgac
    16141 cttcataaga agtccctcca ccaaaaagtc cttgaaccat tttatctcat cctctctata
    16201 ataattactc taaatccaat acttgctaca aatgatagca tttcgtgtgg taacatgatt
    16261 gccatcgttc ctgttttatt tactctgact tcaatacttg gtttgttgat gaaattttac
    16321 caattgattc catcgatcgt ttcatggaat ctgtatttac tattttcatt gattgtagag
    16381 attgatttat tccctctgtt gcacttctga tacctttagt atcaaagttt tgcatgattg
    16441 ctcgctgagc tgataaagct gataatgttt gacctattga ctctatcgac ctcttcatgg
    16501 aatctgtatt taatattttt attgattgta gagattgatt tattccctct gttgcccttc
    16561 tgataccttc agtatcaaag ttctgcataa tttcttgctg gactgataat gtttgaccta
    16621 ttgactccat cgactttttc atggaatctg tatttaatac tttcattgct gatagagact
    16681 ctgacaattt agtaacacct gctaaatcga tattactaaa ataatttcca atatatcctt
    16741 caaaatcatc tgacatatca ctaaggtcct caccagcaat atctccagcc aatatttttt
    16801 gaaaagcttt ctctagttga tttgtatatc tcttcaattt ctttattaat ttttggtata
    16861 aagccatttc tatttctttg ttatggctta caatattcct accttctctg attaaactca
    16921 tgtctgtttg aatttcttct agcttaacat ccatatattt actaaatact tcactccaca
    16981 aagaatatga atcagatata gtgtgactgt tatcttgaaa taacttcaat aattgatcct
    17041 gagatatttc tttatacctt acaatattct cttcattttc ttgtgtaact aaaatataat
    17101 tttctccaaa gagatattct tcaaaatcat ataaatctat ccattgtaaa agattctttg
    17161 agtcaaactt tcctattgct tgtatttgtt ttctttttat tgacgaaatg atttcatttg
    17221 gaattgattc ctctgcccag ttatcagaaa aatatgccgg agctaataaa tagattaatt
    17281 ttcttaatgc ccattcgtaa ctatgcaaat acggtaatag ttttttcgaa aaaaacaacg
    17341 catttgtatc taaaagggta gtgtagttta attttgattt acttgcttca atattgacct
    17401 tagctaggaa aatttgaaat atatgtacct cttttattcc tatcttacca gtcttattat
    17461 attgaaatac tacgtgtaac gaatcattat agttctttat gtcaaagcca aatttataat
    17521 ttttttcctg ctcctgaaat cttaagatag aatcactaat atatggtgac aacactttca
    17581 gaaaggtttc ttcgatattt tccattcttc cttttttagg ataatataaa tattcaacaa
    17641 cttgcttact aatatgtcat caacttcttt cctttcaaca attttaattt tcctattaac
    17701 ttgatatttg ctacataagc cacaactagc aaacagaaag agaacttttt ctttaccttg
    17761 ataactgtgt taattactgg tttctttttt ctatttcttt atagtattgt tcttctactt
    17821 ttaatatttt tttaacttct ctttttatgt cagcatacat agatatactg ttgtcctttc
    17881 ggttgaagca gtgataattc tcaccatcaa actcataaaa aagcatatca cttgagcttc
    17941 catctgaatt aattaactga aatttggtat tgagtagtaa ttcatttttc tcgtcattca
    18001 actttattaa aataggtttg atctctttta aataattgaa atatatttcg gttattacta
    18061 aattaaattt cgctactgta taaacataag agatacgtgg ggaatctcta aattggttat
    18121 atatatcttt ccttaaaaca tctaactcct tttttaattg tccgttaaaa tcaaaatgag
    18181 gcattgctag aatttcatcc aaatcaaagt acactctttg ttgatgaata tttactggca
    18241 gatgaccatg ctgagaataa tttctcatat gtaataaaaa cctatacgaa aaatgctcat
    18301 cataaggctt actcagaatt ttattcttaa aagatttaaa atctttttct gctaaaaagt
    18361 ttttcataca aacttcaatg gattcagtca aagttttggc tgaactaatt aagtttatcg
    18421 tcaaagcatt tatctgataa taatactggt cttccgttaa tttttcacct gtactttcaa
    18481 ttaaatcatt agtaaaaatt gtgaagtgtt tgagcaaatt agttaaatta tatctaaaca
    18541 cattaaaaag tttatccaac tctataatat gacgtgttaa ttctacataa tttaaaaata
    18601 ttcttttatc agattctgtt attgtttcta ataatgaatc aatctgcctt attagctcgt
    18661 catcttgtaa attactgaca aaaaacctac tatcacttct cataattata ttctcctaat
    18721 atgatttttt caaaataatc aatatgtaac atagaagtat actctattgt tacatacctt
    18781 atttcttatt atatcagtat ttctctgtga cacataaggg tgtaacataa ttattttaaa
    18841 cgaaatgact acaagtagtc attgaagcaa atctccataa gacatttgta aaatctttta
    18901 aaaagaagcg tcgaaagaag gaacttttct tcaacgcttc ttttattgac tcaattttca
    18961 atattacttg aacgaataat ctgatttcta aactctcgaa aaacttcttg tggttgataa
    19021 tagtctggat ctatttcaaa tgtttctgag aataattcat tgaatttttc tactgaaata
    19081 atgattttac cattcttctc tcgactgttt tctattattt ctgcttctaa ttcaaataaa
    19141 tctagtgacg aactaaaatc ataaatcctg tcatattgaa taaaaaaatc aagaaaaagt
    19201 tgctcaaact cttccttatt tatcactaat gtttcaaacg gaacaacttt attttcttca
    19261 gccattcttc caatttcctc actttcattt tcaaaaatca caaaaatgat attaacttaa
    19321 aagccactaa caaaataaga cttacaaagt aaagggtaat ccaacaaaat aaccattcgc
    19381 tctacgtact acgatttggt agtcctgcct ttcaatcggt aattcataat agtaatttac
    19441 tgtttcttct ttgtcacttt caatatactc tggattatct aatatcatag catactcata
    19501 aagctcttca ccatccttat tttcaatcga ccattgttgt ggacgtattg gtttttctgt
    19561 atcattttta agtgtatagg tcacttgtaa tacacgatca tgttgtgcaa attcatttct
    19621 tttttcggtc acttcaatat ttttaaatgt tagttcaacc ccatctgcct gataagtttc
    19681 acctattttt aaatctattg ttacaatttt tattggcttt tcttctggaa aatgtctggc
    19741 tttaattgtt agataacttc caaaaagaat caatcctagt agcactaaaa gaattccttt
    19801 aacgagccac tgaaacggga agtaaacttt aaacatcttt tttctcctcc gttttgtatt
    19861 tgttttttag agaatacgat aacttttgat caatgtcatc aagcttttca attacttcat
    19921 tacttatcat tgattctagt tctgaaattt tttgttcaaa tttaatgctt tcagaatcat
    19981 taaatttatt atttgatgga ctagctaaca ctggtaaatt tgaaaccata acacgaaaca
    20041 tatttgcctt ggaaccaaaa actcctttgt tttggttgta aaaattgttg aatatttctt
    20101 tagtttcttc tgaagccgag actttaaaat atggatagtt catttttttc ttccaccttt
    20161 ctttttttgg gagggagtct gtagggagct tgctccctca gagatttacc caccatctga
    20221 cttgccagaa agtagcctag cgtgagcggg gcggattgac tggcaagtag atgggtagct
    20281 ggcgtatgtc ctaaaaactg cttctttcca agcaactctt cataggcaaa aaactggaaa
    20341 gtccactaac aagcccattc agaattccat ttcaaaattt tgttgccttt tttgctgatt
    20401 ttcaatcatt tgttgtgtga attcataatc ctttttagca cgatacattt ttacatcgtc
    20461 attcattatg cgactgattt gatagtgaat ctgtttagca gaaggtgtat gagaacaatt
    20521 ttcaaaaccc tttgccggta gaaataactc tttctgatca gtctttttaa tagaaattgt
    20581 tttacttttc gcactacgta aagtttgagg attcacatcc gtatgaagtt taatcgtgta
    20641 ttcttgatca tcttctaaag acattgtgtt tacaaaatgc ttgtctacct ctcgcaagaa
    20701 gtcatcatat ttaatgaatt tttcatcgcc ttcacggtcg ctaaattgct tttgtgcgat
    20761 ggaatctaat tccacaaaag gtacatttaa ctcaccagtt ttgtaattta ctccactaat
    20821 tagcgacata acttgctctc tttccatatt aaaaaaattt tttatatgtg attcttctaa
    20881 attcttttca agttgttcag aaacagattc aaatgaatat cttttcccta actttcttgc
    20941 actaaaagta cgttcgacat actgactttt ttcttttcta gattgtttat ctcttcctaa
    21001 agtaattaac tcacctctac gctcatagaa atttacacct tgtttgtaaa gaacatagcc
    21061 aagtttgtta tttgtttttg aaaattgata aacttcaatg ttatattctt ttgccaaatg
    21121 atccgaaaaa tcttcaaaag aattcacttc gcatttttcc aacgttgctt cgatcgcatt
    21181 ttgcacaaac tcttgataat taatttttcc atatttcatc ttagaaggat cgtttgaata
    21241 aagattggtt ctcttttcta agttttcata gtactcatta ttcactgatt caagaccatg
    21301 ttcattgagg acttgatcgt taatttttcg cattagttct aaatctttag ggtcaatacg
    21361 caacctttta catgtttcca tgttaatatt attaatgaca agatgactat gaaaatgatc
    21421 tttatcattg tgacttgcaa caatgatttg cgaatttgga aaagctttct ctgcaaattc
    21481 gacagctatt tcgtgcattt tatcaatact taactcttta gaagtactct tatcaaacga
    21541 atgaataaca tgaaacgctt gtgctatatc tttagattta ttcatcttgg tacgtactaa
    21601 attcatttct tgcagtgctt cctcactatc atcactacaa tttattcctg agactgcaac
    21661 acatttttct ttttttgaag gattagctgc atattgtaat agcgaggata aatttttctt
    21721 gcgtcctttc gatgttatag tagctcccaa agttgtttca cctcatcttt taaacttgca
    21781 ataatctgaa ttgagtgaat taaattccga tcaacttttt gggaatactc ttctttcgta
    21841 cccggtctag tttgcttttc tatccaatat tcgaaatatt ctttttctac ttctgataat
    21901 ttaaaaaaag agggggcact ccgattggac tccaaaaaaa tgtcatctgg ataactaatc
    21961 atcaactttt ttgctatgct attcgcaagt tcatctttaa aaatgttgta acggctagca
    22021 tcaaaactaa aatgtaggct cttttctttt ttatcttcag attcttctct aaaatattta
    22081 gcaatgatat ttaatgcctt taccgcttga ttcaaattgt taccaatttg atttaactgt
    22141 ttggttaaaa ctaccctatc atcttcagct aacaacggcg gttcatattt gagtcccttt
    22201 aaccgctcac ggcaaacgtc tgctctagaa attccccttt gttttgcaat caatgtcagt
    22261 tcaagataag tctttttatc gacacgaaaa tttaaggcct cactatgttt gcgatttttt
    22321 tcgtacattt tttgtttatc atccattgat tatcccctca catttttttc tagagagcaa
    22381 catttgaaat cgctctctat ttcatttttc atctttaatt cttcttgaat catcagttca
    22441 atataattag tgaccgtgat tttttcattt gttgctttct ttactgattc acataattcg
    22501 tcaagttcta agaaatcatg tgtactaatc tgtagaatta attgcatgac aatcacctct
    22561 ttatttttta agtaaactat cgaaaagtgg attaggatca ttaagtgtat ctctgaataa
    22621 ctcgttgtct tcataggtaa accttcgtct tggttttgat cccttatcat tttcacggat
    22681 ttcactatca ataatcactg tttcatgatt tgaatcaaat tttttcgctg actttctgtc
    22741 ttcaacgata ttgatttttt gtgttttttc tttcatttct ccactatctt tttgaacgtt
    22801 ctcccgaaca tctactgttt tgaatccttg actacttgta gaactttgta cattttctcg
    22861 aacatctact gtttttggtt gacttgcact gctaccttgc acattctctc ggacatccac
    22921 tgatttaaac ccttgactac ttgtagaatt ttgtacattt tctcgaacat ctactgtttt
    22981 tggttgactt gtactgctac tttgcagatt ttctcgaaca tcaattgtct tagtaccttg
    23041 agcatttgaa gaactttgta aattctctct tacatcaaca ttccgtccaa catgaccatt
    23101 cagtgaacct tgaatatttt ctcggacatc tactgatcgg ccaccatgac tacttgtaga
    23161 attttgtgga ttttctttga ctaatttttc taatcctgta tttgaatttt tagcttcttg
    23221 aattctttct ttggtcaacg cttcttttcc gtcaatatta gcgcctgtcc gcccttctac
    23281 acgtttcctt aacattattt cctcgcgatt cattgcttca ggcgtgtatt tcgcttcatg
    23341 acgcttctga ttaattaatt cttcagcaga cttagcttga ccgtgcatag catttttacg
    23401 ttcttcaata tcttgttgga ctttttgctg aattgcactt acaggattat tattcgattc
    23461 agcattattt ttcattgctt cacgcatacg ttcagaacta gacataatac tatcggcatt
    23521 ttcaacctta gcaccatctt ttctatccga aagacgtgtt ggcatggaat cagcatattt
    23581 tccagtttca tcttctttat ttttagctaa cataggtttg ctatttttat taatagcgga
    23641 cacagtacca tcatctaaac cttctttaaa cttactcttg gtatcattag ctatatcacg
    23701 catactctta aatggttttg ttgctctttc actagccatt gtagatccat ctgaagctaa
    23761 acctgataat ccacgttcat gagcataccc aagcgctttc cccgttttat tagctttttt
    23821 agaaagtgat tgtccaaagt tgcgttcagg ttttcgttct tccccttcag cttgatcgtt
    23881 tccaccatta tttttcccac ctttagaccg gttcatacca cctgttcctt tacggaatag
    23941 cattgcaccc attccaaaag cagaagcaag ttgcccatag ccttctttga gtccaatatc
    24001 cacaccaaag tatctcatga ctgttccact gcctttgatt aaaacaaaca cagcacagat
    24061 ataagcaatt gtttttataa aaatatttgc tgagatacct tcgccagtat ttaggtaatt
    24121 tatgaacatt gcaaacatag ataaaccgaa cccttgaaat ccgactgtca aatagcactg
    24181 taaaatatct gacaaaactt gttttgatcg ttgtcctgtt tctaagtcag tagcaaatac
    24241 taaaactccg agtacacgtt taaatgctaa ctcgagtatt gctgtaatta taatgaaagc
    24301 actaaaaaca tacgctaccg ctaaagcaat caatccaaaa gaaattcccc acaggttcgc
    24361 ttgatagcga taatatcctg atttgatacc atcaacaaag gttgataaaa aattatctga
    24421 aaatttagta gcaacaaatt cattatcaga attttcaact aattcataac gcagattatc
    24481 agctgttggg ttctcttttt ccattttatc aattacatta tctgtcaata cttgcgtaag
    24541 atccgttttc cagaaatttt cttttgttag attatttcgg tcatttgttt tatcaattgt
    24601 cgaatattga tctgtcttat taatataagc taaatctgtc actccctgct caatcaatga
    24661 ccatgctaca ccgccatctt gattattgat actagttgcg tcattataaa gagtctttgc
    24721 aaacattatt cctgaaagtt tttctgttga tccaatcaaa gtcggcatta aaagaattaa
    24781 tgctacagac atcactacgt tcattccaac cgattttaaa tccactgttc cttttccagt
    24841 aatcattttg taaccaacaa acatcagtga aagtatcatt aaaccaataa caactgtact
    24901 taagaccgat tgataaactt tattcattcc cgtactttca ataaagctaa acaaatctaa
    24961 cacttcggga atcatatttt gaataccatc tacaattgca aacatcgatt ttataacaga
    25021 ccaccctatc cacaatccaa actgactgta aaaatttccg ttatattttt ttaaatacga
    25081 ttgatagaca ggattattca aaaattcagt ttgactttcc gtgtcatcaa accaattgaa
    25141 aatgcttgct tggacaacat taccggtgaa aacaacgata aacattaaaa caagtatgct
    25201 caaagctaac cagtgtattt ttctactggc aatcttttct ttaactattt tcattattac
    25261 ctccctcagc ccattttctg agactctcag agagcttctg agctgttttt ctgtcaatta
    25321 tgatatgagt tacacaattg tcattttcag atcttaaatc gagaactata gtgttgaagc
    25381 aaatagtctc gattaattcg agattatctg tattttgatc tagacaccaa acttgatcta
    25441 ttaaacgatt ttcaatcaaa ataacccctc ctatattcaa agttctcaaa tgaagcggag
    25501 atacccctcc acttcatttg actcaattct attcttattg cttatctatt ccttcactga
    25561 tttgcaattc aattagttct aacgctttat tttttagaga agcaaagata taatcgtctt
    25621 tttgtttgat acggtgatct gtttttgctc ttcgtatcaa gtcacgtaaa gtcattgata
    25681 gctgttctct aacataatct acaaaatact tgttattaca gatttcatag ataattttca
    25741 gttgaatttt ttgttctttt tcagcagatt taatcgcata tttgaatttt ttataccagt
    25801 cataaaattg actgtaatcg ttattaccca ctaatttcag acagtttaca atattatccc
    25861 cccatacctg ataaagccat tctttttcaa ttaatgattc aattaatatt tcatctaatt
    25921 ttttattttc aactgaaagt gaatcaataa agtcagcatt ttgagattct ttaaaatctt
    25981 taaaatcttt agaagaatga ttaagttctt gtaaaaattt ttcacctgga agcgggaatg
    26041 acttatcacc atcgaatgtt gatttgtcgg aaggtgtatt tttttcactt ggactgtaat
    26101 gttgatttgt cggaaggtgt aaattttcct caatgcactt ttttttgtag aaatctcctt
    26161 cgctgaattc tggtttcaat aaataataaa aatttggatt attcttccca gtttttttgt
    26221 taaatccatt ttttttaatc gtaattaatt tatattcttc tagctctctc aaagcgctta
    26281 ttaccgtttt ctttgttaaa caagattttt cagctatttt ttctatagca aaaatcaaaa
    26341 aaattcttcc tttgctgtca acttttgaat tctctaaaga atattcattt tgttttttca
    26401 aatacatata aacttttttt gtactttgtt tcatcttctt atatttttta tgtttatcta
    26461 aaaaaatcgg atacttcaag tatttttcag catatacgtc atcttttgag tatgcatact
    26521 gagcaattaa tttatctatt tcttccattt attcctcttc tttctaataa atgttaaaat
    26581 gtagtcaaat aaagttttct tagaacttta ttgtcgagtg tttcctgtgg tcgccaaact
    26641 gtcgggaaca ctccttttaa tttttttcaa agtattttta tattttctat acttcatcat
    26701 tattcaaaca aacacttcct tttttctatc aaaagcacgc cacgcaactt tttatacttc
    26761 atattctttg ataaaccttg ctaattcttc ttttctaata aattgacaat ctccgattat
    26821 gcttactttt aaacctttct ctctgataaa tttttttaat gtacttctgc tacagcttaa
    26881 atacttacaa gcttccccca atctaaaaac ttctttgctt agagatgttt caattcgaac
    26941 ttgatctact gcttcttttg caactcgata gaaatatctt cgcaattctt gatctaaatc
    27001 cgatggaaga ataacttgaa tctcagattt cattttcttt gcccccacta atttgactac
    27061 atgtattcaa attcaacttt tcgtcacttt tcattaaaaa aaatttgatc aattgtgata
    27121 ttttcaattc ctttttcttt cacaagatac ttcaaaataa tcatttcttc tggagaatag
    27181 tttgttttac cgctttcttt gtttcttacc gcttgtactg aaatgccata tttttcagca
    27241 agttcagatt gtgataatcc acaaaacttt ctaaaatctt taggcttcat gactcctcac
    27301 tcctccttct tttcaacttt tcgtcactaa aagaataaca tatatttatt cataccgcaa
    27361 cgtaaaattc aacttttatc tactttttca tttcattata tgagaatgta gtgtaaactg
    27421 tagaatagaa tagatgaagg tggtgaaacc attttgtttg ataaaaacat taattctgaa
    27481 attggaaaac gaatacaaga aatacgtgtt tctttagggt atactcttga agaatttggc
    27541 gaaaatctga cacctcccgc aagcaaaagc aatgtttcta gatgggaaaa aggaaagaac
    27601 atacccaaca aagcgcggct actaagtatt gctaaacttg gtggtgtttc tactgaatat
    27661 ctactgacag gtaattctca aaaaactaag aaatatatca agtatttatt caatgagatt
    27721 ttttttgaca acggtacctt ccataacaaa ttcaaagatt tcggtaaaga cagagatagg
    27781 cttggtctat atgacttatt tttagcagta tcgaaaacat atagtctaga taaatcacct
    27841 tctttaaaag ataactttgt agaaatgatt atagatgatg tatataaaaa actcttgcaa
    27901 actgataaat cagatgacca actcatcctg ggtttcatat atatttgtct tgatgaaaaa
    27961 ctttcaaaca gacagaaaag tcttaaaagt atcaattatg aggtattcaa ttatttaggt
    28021 gttgcatttg gtttaattga tagcgaaatc aaatctacaa acgcaggtaa cgatgaagat
    28081 ttattaaatt atttaaaaga attaacatct tttattgatg aaataaaaac aactacattc
    28141 aacaaaaaaa aatattttca aaatcaatat gatatagaag aataaagatt acattttcct
    28201 atcaaaagca cgccacgcaa ctgataggag gaaaaacaat ggcaacaatt aaacaatatg
    28261 aaaagaaaga cggaaccaaa gcttggatgt ttcaaacata ccttggaatc agcccgctaa
    28321 ctggtaaaga agtccgtact acacgtagaa attttaaaac aaaaaaagaa gctcaaatag
    28381 agcttaatag actacttgta gactttgaag agaatggact taaaagggaa acgaacgaaa
    28441 ctttccaaga agtgtatgat ctctggtatg aaagctataa aactactgta aaagaagtca
    28501 ctcaattaaa aattgagatc aagtttaata aatggattct tccaaaatat gggcagttta
    28561 gaattaaaga aattactaca aaacatgttc agaagatatt gaatcagtgg gctaaaacaa
    28621 cagatcaata taaagtccta cattctaccg taagccgtat tttcaaatac gcaatgactc
    28681 tagggctgat cactaaaaat ccctgtgagc tcgtaatcat gcccaacaga aatgttgaaa
    28741 aaaagaagga tactgtcaaa atttattcac gagatcaatt agaaacctta tttcaatatt
    28801 tggaatccaa aagaagtacg tatagggcag agtacgataa aactttactg cgctttctat
    28861 tttttagtgg ctgtcgaatc agtgaggctt tagccttaga ctggtcagat attgactttg
    28921 attcaaacac cgtcactata aacaaaacct taagccaaac aaaatatggg tacaaaattt
    28981 caagtccaaa aacgagaaat agctatggtt ccctatcttt agacaataaa accattaatt
    29041 ggctaaagaa atggcaaatc aatcaaaaaa gatatatgtt aaccctcggg attactgatc
    29101 caacaatgat tttttgtgga atctacaaag aaatcattac taaacacgct atctattctc
    29161 gtatgatcac aatctgcaag aattctggta ttccatttct aggtaaccat gtaaccagac
    29221 acactcatgc tagtatgctg ttagagtcgg gagtctccat gaaggaagtt caagaacgcc
    29281 ttagacactc atcaatcaaa ttaacaatgg atacctatgc tcatctaact aaagaaacca
    29341 aagaaaaaac cgttgaaaag cttgcagagc acctaaattt ctagcggaac gtattcaatc
    29401 ccgtattcaa ttcaaaacaa aaaaaagctg aacccttatc taccaagggt tcagctatca
    29461 tttacagctt attcttcttc ttccgccatg aatgtattga atacttcttc aatcatgtcc
    29521 cattctgcgt ctgtttcgat ttgttctaac tcgccttctg ttccatcttc gttttcgatg
    29581 tatgcataag cttgtaattc tacatcttca tcttctgaaa caccagcagg atataaaaga
    29641 acatagttac gtccaaattc ttcttgtccg tcaactgtta gtaggatttc atataatgtt
    29701 tcatttcctt ggtcatccac caatgtgatg tgttcgtgtc cttcatggtc gtgggaatgg
    29761 ttatgttctg tcataagatt acctcttttc ttttattatt aaaataattg cttacctgag
    29821 ccgtctaaat aattttgtaa gatcatcaca gctgctaatt tatcgatgac tttttttcgt
    29881 ttggctcttg aagtatttgc ctgctctact agcatacgtt ctgcctgaac agtggttaat
    29941 cgttcatctt gataatcaac tggcaaggag aatagttctt tgatcttttc tccgtatgac
    30001 atggaagctt ctgcccttgg tccgatggaa ttgttcatat ttttaggtaa tccaactaca
    30061 aaccggtcta cttcatattg tgctactaat tccttcaacc gatcaaagcc aaactcttct
    30121 tctgcttcat tgatccgaat gatctctact ccttgagctg tccagccaaa ggggtcactg
    30181 atagcaacac ctactgtctt agaaccgaca tccaagccca ttactctcat aaattcactc
    30241 catgatttga gaggtagtcc ttgacaatta cttccatgat ctcatcgcgt tcgtgacggc
    30301 gaatcaaatt tcgagcatca cgataacgag gaatgtaggc cgggtctcca gaaagcaagt
    30361 agccgacaat ctgattgatt ggattgtagc ctttttcttc caaagcccga taaacaatcg
    30421 ctaatgtttc gctcacttct tttttgcgac tatcgtcaaa accaaaacgt acggtttcat
    30481 ctgtaaaacc catgattcta cacctctttc ccgcaaggta attccagctt accttctaca
    30541 accttatttt actcgaagaa agggaaaact acaaacaaat attccaaact tccgaataaa
    30601 ttaagaaaca ctatttgtat gtttttttcc ttttttctgt agctttatca aaagtactct
    30661 tttcataaag tatacgctat atttctttga atgtatgttt ttacacaatt ttttcataat
    30721 attcataaaa aattcgaata gctgctacta attttttgtt tttgttgctt gtcctgtctc
    30781 ataattgatc gtgtagccat cttgaacatt gtagataaag acattgaatt caattttttt
    30841 gtcttcaatg ctttctgctt ccaattgcac tcccctggct accagttcat tcccctcaaa
    30901 atgaggagtg acacggtagc gtacatgatg atttgtttct ttgatatatg ccgcaatatc
    30961 attttcgtga gcaagcatgt atggagcatt caacgaacgt gtccctgtca tcaagttttt
    31021 aatgttgtta ttttgaccag tcagctggaa accaattaaa tggctgcgat tatataacca
    31081 ttgcccgtca cttaatttct tttgtttcca acctgtcggg tcaatataca aaggctctct
    31141 cttttcagta gggaacaaat cttttccaag catagcattg gcttgtccta ctctgttcaa
    31201 tttatcaata tttgaatagc tttcccatgt tcctttggaa aggctaagat cctcttttgt
    31261 aaattccggc tgattcccat tgatttctat ttcttgatgt tttccatcat attctggcac
    31321 atccgctact tgagaagctt gtggtgtgga atcaaaaata gttgtaatct gctgaaagat
    31381 atctgaatct ttttgtgtgg tggaaacacc gaatgcccct gcaatcacag cagcgacgat
    31441 ccccacgatc gccttggttg tttttttgct catttcttta ctcctttact aacagctccc
    31501 taaatcttaa catatgttcc cctataaata aatactagaa agaaatagaa acagcaaaaa
    31561 ataaaacctg ttttaagatg gcttaaaaat acaaaaaaga gacggattcc tcgtctctaa
    31621 tcagtcacca ctctatctgt aaggtattta ttattattca aaaattttct tcatactcaa
    31681 gacaatacac tgatttcaca accgcttggt aaacggtgcg caaaagcttg catctgcggt
    31741 aataagtcca tccaagttaa aaacataaga agttgttttt gcttggctgt tcttattact
    31801 tgttgcagag cagctttttc acaacctatt ttaagcgggt gacgggaatc gaacccgcga
    31861 catcagcttg ggaagctggg attttaccac taaactacac ccgcaataca aaaacagtat
    31921 acgcctcttt tattccttcc tcaagttttt ttattcttta cgcttctggt atgttttgta
    31981 gtaaaataaa actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga
    32041 tctgttgctg gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat
    32101 gtatcgggta gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt
    32161 cagctatgtc actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat
    32221 ggaacaaatt accaaccgtt cgattaattt agaaaaagta gtaaaagtaa atgatctttc
    32281 acgtaaatat gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat
    32341 cgaccgagac cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag
    32401 tggaacaatt atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat
    32461 tggaggattg ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt
    32521 atcggaattt ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg
    32581 cttaggaagc aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt
    32641 tcaaattacg aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg
    32701 cggaacagaa gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca
    32761 gcttttttat taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag
    32821 ttttttagcg actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg
    32881 ttgtggtctt tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg
    32941 atctgctgct tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt
    33001 ctctaaacgt ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt
    33061 ccctggtggt ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc
    33121 ggctcattat gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt
    33181 atcagaagtc tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat
    33241 gaaacgaaaa aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga
    33301 gacgttcagc cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg
    33361 agcaataaga agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt
    33421 ttacataaaa ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa
    33481 aggggctgcg acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac
    33541 gttcgttgat aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga
    33601 atattcacta accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata
    33661 atactgatga tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg
    33721 ttaaataatt ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa
    33781 taacgtagtg acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa
    33841 aacaaaggca gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc
    33901 tgcaatatcc atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa
    33961 gaacggataa acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc
    34021 tgattaactg ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta
    34081 ttatctaaat cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt
    34141 ggagttaaca aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga
    34201 gcattgtaac taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt
    34261 ttgaactcac tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt
    34321 acagcaagga aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt
    34381 tgagaaagtg gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga
    34441 tctgttaact cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat
    34501 gcgatcagca tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac
    34561 actgctgttc gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa
    34621 aatagagaaa acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc
    34681 catccggtta aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca
    34741 actaccaaaa tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact
    34801 tgcaggtcat ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct
    34861 aacataccaa aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata
    34921 gcaaacatag caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac
    34981 aaaagataga tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc
    35041 tgtggattaa catataaaag attaactcga attaattcta ataattgtcg cttatacata
    35101 cggtccatct cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc
    35161 atcaggtgag ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt
    35221 caaaatagcc agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat
    35281 gactgttttt ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg
    35341 aggatcaagt ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt
    35401 gccgatcaaa atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg
    35461 atgactatac atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt
    35521 gtctaattca taggcagctg aaatcaagtc ccaatattct ccagccgtca attgtaaaaa
    35581 aatatctggg gaatcaggca cataaccgat tttttgtttg atactcaatc gattttccgt
    35641 caatttcatt ccatctacta aaatcgttcc cgatgatggt tcaataatac taactaaact
    35701 tttaatcgta gttgattttc cggcaccatt atgtcctaaa aatccaaaaa tttccccttg
    35761 tcttatcgag agagacaagt cttttaatgc ttgttttttc ccgtaacttt tacttacatt
    35821 gatgaattca atcatttctg tactcctttt gtttgttatt actttttatt gtacccttcg
    35881 ttattggtgg agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa acgactaggc
    35941 tgctacttgg gttgttctct tctacaggaa aacactcttc taacaggtaa gatatgagtc
    36001 tttgttttct ttaaaatgat tcattttcta tcttttcttg cataaaattc tttagtttat
    36061 caatacttct ctttcccatc tctagttgtt ctccatttgc aaatgttact atttcgttta
    36121 ctctgctaat agatgtaatg ttttcagtat taatgatata agcttttaaa tctttacata
    36181 aatactcata ttcgagtgat tttttatatt ttttaattgt gccgtttacg agaatctctt
    36241 catctttcaa tactaggcct attttatttt tataacctgg catgctcatt agataaagga
    36301 tttcatcgta atggatatag tgatcgctat taccataacg gacagttgtc cacctttttt
    36361 tcgtagcatg ttgaacagga tagttttctt tttctttcaa taagtaactt tggatgcttt
    36421 ttttattttt ttcataagtg aattctttta tcaaataacc actaggcgta atctttcgat
    36481 taataacagt tattgcttta ttcggatgag acgttagata aacaatttta cacttttcat
    36541 tcttttctct aagcatttcg cctagttcga taccgctata ataggcgttc aaatcgatgt
    36601 ctaggaaaaa aatatctgag gaatagattt cattattttt aatgaattca acaaattgta
    36661 taacgttcaa tccctgaacg atctcaaatt tccaaggact taataaaatt ctggattctt
    36721 ccaaaatttc tactagcatt ttttcaaaaa ccaaactatc atccaaaata aatatacgcg
    36781 ttaccataaa aagtacctcc actcgatatt ttactatcaa aatcatttca tacaactttt
    36841 caacatacat gttaggaata aattgtaatt ctcttcacaa tcttttccaa cacctttgaa
    36901 accgatatca aatactttca ggatgctgct tctaaaataa atatcaaaca aatgaataca
    36961 tcatgcaaaa aaagccagac aaccgacgtc cttgcgtgtc agctgtctga gctatgttta
    37021 tttattttgt tcttttaaaa agaacgatac acatactgat ttttcggagc agagactttc
    37081 tcagcgtttg taacaactac agatgggatt tcacgcttga atggttgata atagtctgat
    37141 tggatggttc cttctatttt ataccattca tcatttttaa ccgttactcc ttccggcata
    37201 tgcgtcaaca aaccaaagac tcctgaatct gccacacaat ggataatgcc gaatcggaac
    37261 aagaagatat ctgtattgct atcgttcggt gtttggtaaa caaatccttc atagctgatt
    37321 tttttgccaa tgaattcgct agggtaatta taaattagct ccataacttc taaatagttt
    37381 tcatctgtga tcttgatagt ttgcttatcc ccatactttt ctttcaactc attcatttgg
    37441 tctaagtagt ctgatttgtt gaaataaata ctggtatccg gctttaagta ttgtgtctgc
    37501 atctccggat cccctactga ttctttactt actgggaaat taaatccttt ggcttctacg
    37561 atcgtagtgt cgagactaac tgttggaaac aacgtcccca cgattactgg caaagctagc
    37621 agtacataag cgattccacg ttgataaggt tttgacaacc catggtcatg atcgtgctcg
    37681 tgtgcctttt cttctgcttt tccgtttttg acccacaaat acagctgaac taaagccaaa
    37741 agagcagata aaatcattga aagaatagct aagtagcgat aatggacatt gatatattga
    37801 tcgagtttgc cagaaacttg caagtacatc atcaattcga aatacccaga taaaatcaaa
    37861 aaccgcagca tattttccct cctaaataaa taatgtgaat ccaataacgc taagggcaat
    37921 cagtccgatc agcgataaaa taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt
    37981 tttgatatcg accattgggc caaagaccaa aaaggcagct acaggtgctg tgccgaacag
    38041 acttaacaaa gaagatccta taaacgcatc tgcttctgag caaagagaca tcgtaaaggc
    38101 caataacagc atgatgataa tggctaaaat ctttgttgag ccgagcgtta gcataattct
    38161 agttggtaaa taagtctgca tcgcagctgc gattaatccg ccaatgatca aataacgacc
    38221 tgtatcaaaa aattcgtcga ttgaatgtgt caacacatgc ccagtttgat ggatcagctg
    38281 attttttggt tcattcttct cactttgttc ttcttcatgg acatgatgat ggtgattttc
    38341 tgctacttta gacgtgattt tttctttcaa tacagcgtct ttttgaaaat aggcaagcca
    38401 gacaccaatc acaagggcaa caaaaaagct gcctatcacc cgccagcttg ccatctgcca
    38461 agaattgcca aatgcgataa aagtggaaaa aatcacgatt ggattgatga tcggagcagt
    38521 cagcataaaa gcaaacgctg tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat
    38581 cggtacgatg ccgcattcac atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat
    38641 agagaagata cgattttcag gaagccatct cttcacacat tctggggtca aaaatacttg
    38701 taaggcgcct gagataagac agcctaataa tacgaaaggt aatgcttcaa taacgatcga
    38761 taagaaaatc gtactcatct gcaaaaccga atttggaata aaagaaaaca tgagtttcct
    38821 cctttttatt tctagttcat tcgctttgca aatatacgct tttattcaaa aaaaacaagt
    38881 tgctctgtaa atttttacac aactcttatt ttttaccaaa gcacttcaat tgactttttc
    38941 tgttattccc attaaattaa ggtctcatac atatcacttc tatcataaaa gggagaataa
    39001 aaatgaaaca aaccactaac ttgactgaag ttcaagacat actgcaacag tcatccgttg
    39061 ccgtcattta tctttcgatg cctaattgca gtgtttgtca cgctgttcgt ccacgactag
    39121 aggaacttct cacacattat gatattccgg cacttcattt ggatgcattt gaaacaccag
    39181 aagtagctag taaatttgaa gtgctgactg cgccagttgt cctgattttt catcaaggaa
    39241 aagaagtctt tcggcaagca cggttcatcg actttaaaaa aatccgttat ctactagatg
    39301 aactgacagc agaagatgat tccctttctt atgaagaaat cttccgaaca aaataatcgt
    39361 tgtccaaaca gaaaaaagcg attgaacaag tgaaagtttt gccttcattt gttcaatcgc
    39421 tttttatttt tattctgctg tattttcgga atgcgcattt gcgtcagaat gcaagaaata
    39481 gatcaaggtt tgcaattcat tcgtcaaatc aacattttgg atcaatacat ctgacggagc
    39541 attcaatcga gcagcggtaa agttcaagat tcctttgatt cctgcttccg ttaactgatt
    39601 gactacttct tgtgctcttt cagcgggtat cgttaaaatt gctacttcga tttgttggat
    39661 cttgatttgt tctgacatat cttccattgg ataaacgggg ataccgtcta cgatgcgccc
    39721 aacgatttcc tcattcacat caaaagcaca actgatacgt atgctattac tttgatggaa
    39781 tttgtatttt agtaaagcac ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa
    39841 ttgatcgtcg tttaatgtct ttgcaaaaaa gtgcataaga tcttctacat cataaccata
    39901 cccacgtttt cctaattcgc caaaataaga aaaatctcga cggatcgttg cactatctac
    39961 ttgaacggct tcacttaatt cagttgaaga cacttttttc tttccagcat tatttaaaat
    40021 ccttaaatag cgataataca aaggcagtct tttagctgtc gcctttggaa taatatgatc
    40081 tttcacgaaa tgacctccac gtattgtcta attacatctt tgatttctcc atcatagcag
    40141 tatttatagg aaaaaatcaa ttccattgct cagatattgt gaaattttat tctttattca
    40201 tttatttatc atttctgcaa atctcgcagt ataatggaat ttatggtaga ctactaattg
    40261 aaaaatagtt ttttatttga ggagaattca caaagtagaa aagaggattc catgatatta
    40321 ttacaagcca atcagattgc tcgccatttt ggtgcagaag tcttatttga aaacatacat
    40381 ttagaaatca gtacaggcgc tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg
    40441 actttattaa agatcattgc tggaattgaa gcacctgatg aaggaaagat cgctaaaaat
    40501 aaaacagcga ctctcggata cctcgctcag gacacaggac ttgattccaa tgaaacagtt
    40561 tggaacgaga tgctgaaagc ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag
    40621 ctagagattt tcattagcga aggtacgcca gacacacctg cttatagttc ttatttaaaa
    40681 gaatacgacc ggctgcaaca cgatttttca gatcgaaacg gctatggtta tgaaaatgaa
    40741 attcgttccg tattgcatgg gtttaaattc gattcttcat tttatgatca gaaaatcgat
    40801 actttatcag gcggacagcg cacacgttta gctttagctc gcatgctttt gcaacaccct
    40861 gacattttga ttctagatga accaacgaac catttagata tcgagacact ttcctggctt
    40921 gagaattatc tgcaaagcta tactggtgca ttattgattg tttcccatga ccgttatttt
    40981 ttagataaag tagtcaatga agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa
    41041 ggtaactatt cgaaatatct agatttgaaa gcagaacagt tagcaagtga atggaaggct
    41101 tatgaaaagc agcaaacaga aatccataaa ttagaagact tcgttgctcg gaaccttgtt
    41161 cgcgcctcta ccacaaaacg agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg
    41221 ctggatcgtc ctcaagggga tgaaaaacaa gcaaactttc tattcagtat agaaaagcct
    41281 tctggaaatg tagtcttgca aatcgaagat gccgctatcg gatatgataa tgtcacctta
    41341 tctgaaccaa tcaatctaga tatccgtcgt caagaagcga ttgctctagt aggtccgaat
    41401 ggtatcggga aatcgacgtt gctgaaatca atcattcacc agatcccttt tatccgtgga
    41461 aaagaaacgc tagggacaaa tgtcaccatc ggctattacg atcaaggtca agctgatctg
    41521 catacaaaca agacgatcct agcagaattg tgggatgagc atcctaccac tccagaaaaa
    41581 gagatccgaa gtgctttagg tgctttctta ttttctggcg aagatgtaga aaaaccaatc
    41641 cctctgctaa gtggcggtga aaaagcacgg gttgctttag ccaagttagc gatgaacaaa
    41701 gaaaattttt tgattttgga tgaaccaaca aaccatttag atattgacaa caaagaagta
    41761 ttagaaaatg cattgatcga ttatgacggc acactgcttt ttgtttccca tgaccgatat
    41821 ttcatcaatc ggatcgcgac aaaagtcgtc gaactctctg aaaatggcag caaagtctat
    41881 ctaggagact atgattatta tttagagaaa aagcaggaag aagaagaaat cgccgaattg
    41941 ttggcacaag atgagccaga atctgctcct gctcccacga acaaatctga ttattaccaa
    42001 agcaaagaac agcaaaagct tatccgtagc cttcaacgaa aaattactca ggttgaagaa
    42061 gaaatggcgc ggatcgatga atggatcgat cagttggaac aagaaatggt cgcgccagaa
    42121 aacctggaag atcacgtaaa actcaatgag ctgaaccaag atttagaagc tgctagacaa
    42181 gatcaagaaa caaaattgac ggaatgggaa gaactaagct tggaattaga agaatatgaa
    42241 aattaacgaa aaaaccaaaa cagaaaccat tcatgctgtt ttggtttttc tttttttatt
    42301 ttatcaaaca aaaattccat atttaccttt ttcaccaata agaagaccaa ttactagtca
    42361 ttctcactaa agcttctaaa taatagtaat caccccaaat acaaggtgta tttaccccat
    42421 tatttttggg cttattataa acagactgtg ttaaaaagcc atcttcttct tccgttgtag
    42481 catatctttc tttcaattgt cgcataattt tccaaacagc agactggtat aagttctttt
    42541 ctgttttgtc taatagacct gattctatta attctaataa tccacaaacc atgatcacca
    42601 tactagatgt atctttttct tctctgctag aatcatcaaa gatcaaatcc caataacaaa
    42661 tggtatcttc tggcaaccga ttcataaaat aattggctaa tattttgctt aaatcaaaat
    42721 atttttgttc tttcaaataa cgataactta atgcaaaacc gtaaatcccc catgcttggc
    42781 ctctagccca acaggaatta tcactataac cttgatgcgt acttccatat cttggttttc
    42841 catctttgac atccatataa aatgtatgat aagttgaacc atcttctctt actaaataat
    42901 ctgctgcatt ttgaatatga gcctgagcag cttttttata tttttgattt ccagtttctt
    42961 cactcgccca atacaataat ggaagattca tgcaacaatc catgatcatt ctaccttgtt
    43021 gtgaaggatc actaggatct ccccaagctt gtataactcc tgccttctcc caaaaacgct
    43081 gaagtaatgt ttctgcagca tcaattgaag cttccttcat ttttcgattt cccgttattt
    43141 tataatctgc tacggttgat aaagaataca aaaaaccaag atcgtgatga ttagtgataa
    43201 tttgacagtc aatcctgtgg ataaaatcta tatctgcttt ttcagctgtt tgttgaaata
    43261 aaggatcacc cgtcttctca aataacaacc aattaattcc tgtccaaaat ccttctgtcc
    43321 aaccaatatt gtctcttaat ggataacgtc cttttatagg acactcgctt gggaattgat
    43381 actgactgtt actgatacgt ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct
    43441 cattattcca ttctttttcc attaattctt tataacattc atttatccat ttttctggat
    43501 gtattatctc ttcaaacatc actttatcaa gcatttcccc accagccttt agcataattt
    43561 tagtcttttt ttattgtttt tattttggat agttagcccg gttgtcccat caaaatcaat
    43621 tttgggacga ttgttccata cttctaaact ataatcccga tcttgatgag caaccactcc
    43681 tgttccgagc caatgggttc cttttccaaa tgtaccctct aatccaggaa taaaagtgtt
    43741 ttcccatgtc atcaagtttg tattgggcaa acccataata gctgtggatt ttcgtttaat
    43801 agtatcgtca cttaaacaga taatacctga aaagccagct tcactcaccc tatagttttc
    43861 cttgtcttct tctatgatta ttccttcttt ctcaggagaa cattcaatag caaaccctgt
    43921 ctcatataga acatattctg tatctaattc tactttgtgt acgcgaatct gccagccttc
    43981 aaaaggaata agccaagttc tgatttttgc ctgtaaagta ggttcccatg tagaggcaag
    44041 tacctctttc ttacatacaa tgtctcgtac attatttcga acgatgaact gtccataggt
    44101 tttgatgctc tcattcttaa aactggtcgt gatatatgtg tccgctcgct gaatccctag
    44161 cattgaatcc attgccattt ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa
    44221 ttgactagaa taagcaaatt ttaaatattt ttcattggtc aatgtatgga aatttggccc
    44281 taattgtccc gcattcaaaa aaaatgtatg tccattatca tgttgtatga gcaaattggc
    44341 ttctgaaaga agtttagttc cttcacgaat aggcataggc atttctttag ccgtccaata
    44401 ctcatggtct tcaggtagcg ccaacaataa aaaaatctta ttcagccata atggagataa
    44461 cgtactatta tagggttcag tcaatgtcaa ttgagggtag gtataaccaa tattcaggat
    44521 acctttttca tcaaatattt ctttttgcat ccaccatctt atattccgat tgactaaccc
    44581 cttgatttca cctaatttaa aaggcaaaag atcggcccac aacatagctg accaaaagct
    44641 tgaaactgca aaacgataaa tcatgcttcg accaaaaggc acatttgcac catcttccgc
    44701 aaaaaaatga atatagtctc tagaaaaatc aatagctcgg ttcttatata tctggctaat
    44761 gggcttttct ggttctagta cgctataaat caaaccataa taatgaaatg caaaaggatt
    44821 ataataatct tggcgacctc ttgaactgtc tcgataccag ccattttgaa gataacaatt
    44881 ttctatatta tcaaatgctt ctttcagtgc tgattcttta actggataac caaatttttg
    44941 gataattgtt ccgacaatta tcttaaaaaa ttgccaatta ccatctgcac attcatattc
    45001 aaaaatttgc gagaaccaat gaataatctg gcttttttct cctgtatcta attttttcca
    45061 taataattgt tcatgatgat ataacgctaa taacattgcg ggcatttcta ccatacgctg
    45121 gtctctttca tgtatttctc cccaataatc gggatgggaa ggatctgttc cagcttttat
    45181 tcctgataga attaatttca accatcgttc atctaattgg tcaattgcgg gccccgctcc
    45241 ccataaaacc cgagagaatc cttccattcc tgcaatttta tttccatatc ctaccccttg
    45301 atgtatataa tcaatttgtg cgccacttgc tcgataaaac ggctggagtg gttgatttat
    45361 ctttttaaat aagtcaaagt aatcctgtct ggtccttaat agtggtactg gatgtttcat
    45421 tgtattttct cctcttttta ctaccaaata gctaattccg actgttctaa tcgaagtaag
    45481 ccttcaataa aaaaataatc agaatagatc agtggcacat tataagtcag ttctctatgg
    45541 tatgcttctg ctccgcctcc aatgatccca tcttcatcca tattccaatt tgcatagtgc
    45601 ttttctgtct tttggagtat attgatagca gcttcatgat acatttttga tcgtacagag
    45661 gaaacatatc ctgctaattc cagaagaccg catgcagcgc atactccagc actggtatca
    45721 atattcgaat cttttacagc aaaatcaatg agaggaatat aaccagtttt ttcgattttc
    45781 tgaataaaat ttactgctat cttttctgct atagctagat agtacggatt ttttgtgtaa
    45841 cgataactta acgcgaatcc atataaagcc catgcatttc ctctgctcca acaagaattc
    45901 tcattgtatc cttgcccgcc aagtaaccga atatatttcc ctgattctgc atcaaattct
    45961 gcgatatgcc cggttgatcc atctttcctc actagatact tagctgttgt atctgcatgc
    46021 tgccgagcaa ttttagagaa cctcggatca ccagtttccc tcgaagccca atacagtaat
    46081 ggtaaattca tcatactatc aataatcatc caaccaggtt tatggtcatt ccatgctact
    46141 aaatagttcc ctgaaatatt atagcgtcca gcaagtaccg tagctgcatg taaccctagt
    46201 tgtcgactat gtttgttttg agactgttga taatgtgcaa tagccgatgg taagaataaa
    46261 aaacctacat catgatgaac gtctgaaaaa tggtacaggg cagattctaa taattgttct
    46321 tgttgtattg ctgcctcttt gaacttttct cgttttgtct tgtgaaagag ctgccataaa
    46381 ataccactcc aaaaaccatt ggtccaccag tcgattcctc tgtcctttat tacgtctgta
    46441 tattttccgt tgtaagaaat atatggaatt ttattcccca ttctgtctaa ttccgcatct
    46501 atcttttcag aagctacatt caaaattttt cttgaccatt ccttcgtctg ttcattcatg
    46561 gatatcccgc cattctttta atttttatga atgtggagtt gctcctagaa cgaactagat
    46621 ggttaacatt cgttattttt tttacttatt tgcagctcca atgtcattct ccttaaattt
    46681 agactgcgcc cacacataaa tcaagaccat tgggatcact gaagttgcta aaattagcat
    46741 aattgcgcca taattaattg gctcacctgt actagaaacc gtcatatttt tgataattaa
    46801 gggaaccgta tatttatctt tatctgaaag catgactaat ggaaatagat attgattcca
    46861 acttcccata aagataagta aaccactagt aataatcgta ggtgataaaa tcggtacagc
    46921 gattttcgta aaaatcaccc actcattggc accatcgatt ctggcagcct ccatcagtgc
    46981 tgtaggaaat tgactgctat attgtcgcat cataaacact gaagttgtac tggcaatcga
    47041 agggatgatc acacctgcat aagtattcga taatcctaaa cgactcataa tgtaaaataa
    47101 aggaatcaat gtggtaaaaa aaggcaccat cctagatagc ataactgtat tgaacaacag
    47161 tttattcccc ataaaacgat actttgctaa tgcataaccc gccattgtag aactggctgt
    47221 agctaaaact gtactgatta cagcaataac aatcgaattc caaataatca agggataatt
    47281 atatctcgcc atgatggctt gccagttttt taatagcata tccccaaact tgaaattgat
    47341 tccgccactt aaaatttctt gattactttg cgttgaagct acaatcatat ataaatatgg
    47401 catcaaaaat attaaggcac caaacacaat aatgacgtac gtaaaaattc tgctttgtat
    47461 cttttgttta gtcattttta tcacctccga atttaaattg aatagccgat aatataacag
    47521 atacaaacac catcgtccag gcaatagcag aggcttttcc aaaatcaacc atttcaaaac
    47581 ttgttttata taaattgact cctacgacca aagctgaatt ttctggtcca ttggaagtcc
    47641 caaagaatat atttgggata ttgaaattac ccaagccatt gatagtggct aaaactgtgg
    47701 ccattaaaat gattggcttt agcatgggga tcgttaataa aaagaattgc cgaaatttcg
    47761 atgctccatc tatttcagca ctttcgtata tctctttaga aatcccttga agaccagcta
    47821 aaaataaaat agtgttatat cctacactaa gccaaatcat cgtgatgatc attgatattc
    47881 gtatccaaaa aggatttgtt aaccactgta caccgttaat tcctagcgca ttcaaaaatc
    47941 cattgaataa cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg
    48001 atgtaatggt tggaatataa tagacagttc taaaaaatcc ttttcctttt aactgtgctg
    48061 aattcaataa aacagcaaag taaatagcta atagcatcgt tattggaatt gatcctgcca
    48121 tgataatcaa aacattcgtg taagatttaa taaacaatgg atctcggaag atagctagat
    48181 aattgtcaaa tccaacaaat tcaaatgagt agccaatttt cataaaacta aaagcaaacg
    48241 agataatgat gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc
    48301 cataagcact ttttttatga gacgtatctg ttgctttttt cttgctgttc tttattgtct
    48361 tgcttggtaa cgaagattgt atcatcgtta agtcctccta atatttttta atcaccagtt
    48421 aattttacgc cgtacttatg agcaaaattt tctgcttctt ttagatatga attttcaaaa
    48481 tcacctgttt gccaatagtt atatgcagcg gtagtcaaat aagacgtagc atctgaataa
    48541 gtggctggta aaacagtatt tgatgtattt tcattgattt tattcaatag ttcatagtat
    48601 ttttgtcctc cttagtagtc aaactttttc tcagctgctt ctgtttgata ggccaatgag
    48661 ttagatgtag ccacgcccag ctccagtgcg ttggctaagc attcttcatc tgtcagcata
    48721 aatgtaagaa attgttgtgc cacttgtgga tttttggcat ctttagggac ataaaaactt
    48781 gatcctcctg atactgcacc ttttcctgga ttttcttttg aaaaaggaat cagtggagcc
    48841 atgcgccatt tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc ataccagccg
    48901 ccagcaacaa acattgcaga ttcttgaaat gctttctcag catcttgtgc accgtagaaa
    48961 tgtacaatat ctgagtcgat catttctttg attaactttg cagcatgttt cgcttcttta
    49021 gtattcagac ggatatttcc ttcttcatca agcaaagatt gattctgctg ggctagcatg
    49081 ttgataaaca attcttggtc cccagattct ggcaaggcaa ttccaaaact accagttttt
    49141 tcttttaatg cttttgccac ctggatatat tcttcccaag aactgatttc ttcaaaatct
    49201 acacctattt cagcaaattt ttttggttga taataacaaa gtgctggggt aaaatcacta
    49261 gcccaaggaa taatcgtttc attactcgtt tgttgacgca aaatattcaa tttttgatca
    49321 taaaactgat cgccatattt gtcataaaat ccatattcat cagcactata aaaagcatct
    49381 tgaaattttt ccagcacacc tgctgctgaa gtgtctgcca tggagataat atccggcata
    49441 tcttcgtgtg ttaccaattt tggggtgatc atttgtactg ttgctgttcc cgaagctaca
    49501 ttcacaattt ttaagtcaat atttgctcca gttttgcggt tatagatttt tgcagcattt
    49561 tcaacatagt ttgtcagttt gccgtttgcg acatatactt ctaatgtagt ggcttttttt
    49621 atcattggat ctgcgatgtt tgctgttact tcttcgttag tttttccaca tgcacttaag
    49681 ctgaataata gggtaatgtt tagcaaagct atcaccaatt tgtatctttt tttcatgtct
    49741 atcccttctt tcttttcaag ttcttatata aaacgctttc acttatgtat attaacggaa
    49801 ttagtaacac aaatatttat ataaaaaaat attttacttg tcgttttggc ttttcatttt
    49861 tgttataata gtcattaatt ataaatagaa agctttttat ataaaaagag aaaggaaagt
    49921 ggttcttttg ttaacaaaca accaatcctc caatcttgtc aaaaaaacac taacctattc
    49981 catagatacc catcttcatt tatctgctgc tccctatttt cgactactaa taaatgaaag
    50041 cgctaattta tatgctgaat ttgcacataa atctatagaa ctagataatc cttccctttt
    50101 attcattgat aaacagcaag aagtccaaat cttttctact caaaaggaaa cacaaacttt
    50161 tatcattatc cagttttatt ttgatgagca attattagca catattacgg gacaatcttt
    50221 ttctatagat caagaaataa catctatcat tacttttctg aaaaatgaac agttcaaaat
    50281 tgattatcta aaacatagta agccagctat ttcctatgat caggaaatca attctttttt
    50341 catactatgc caacagatga gcattcattt gctttcctta ttagttactt attgtctgca
    50401 aaaaattcaa ttgtcttcct taccaaggca ttatcaacag gaacttgctt tattgacaat
    50461 accttcaaat gagaaagtca ttcctaaaaa aaagcatatt ctttattcag atgaattagt
    50521 tgatccaatc atacgatatt tagaagaaca tctttcagaa aattttttgc ttgaacaatt
    50581 agcccaaatt ttttttatca gtccagccag cttaaaaaaa cattttagaa aagtgaatgg
    50641 aaaaagtgtt attagctatt ttaaagaact aaaaatggaa aaagcaaaac gtctcgtgct
    50701 agaaaatgaa ttaacctata cagaaattgc agatatatta ggatttcatt cgatccatca
    50761 tttttcttca gcttttaaaa aatatacagg gttatcccct tcaaagtatt atctatcctt
    50821 aaaatagaaa aaaattttta ggttttatcc acaaattatt tctctatatt ttctgtctat
    50881 tcaaaataaa caataatttt ccggcacaat tatttgctgt tttttaccat aacatagtac
    50941 gatcaacgta gaaaaggaga tgtttttatg aaagttgttg tcattggcgg tgtagctggc
    51001 ggtccttcat ttgccactcg tttccgtcga ttgaatgaag cgcacgaaat cattatctat
    51061 gaacgcggag aaaatatttc ttatgcaagt tgtgctttgc cttattattt aggaggtgtg
    51121 atcactgacc gtgactcgct gatcgaacgt acaccagaaa tattgaaaac aaaaaacaac
    51181 atcgatgtct ttactaaaca cgaagtaaca gcaatcgatc cttctactaa gcgattaaca
    51241 gttaaagacc tatccacaaa tgaagaaata aaaacagatt acgataagtt gatcatctct
    51301 tctggtgcta gaccagatta tccggatatt cccggagttt ttgaagcaga aaacggcttt
    51361 gtcctccgta gtgtgacgga tgcggatcga atcaaatcgt tccttgaaga aaaaaatcca
    51421 caacatgtcg tcattcttgg tgcaggtgtt atgggtctgg aattagctga gaatttcaag
    51481 catcgcggct tagacgtgac tttaatcgat caattgccac aagtcgcttt cccttatgat
    51541 ccagaaattg ctaatttagt ttatgacaaa ttgctgaaag aaggattagc cgttcattta
    51601 gaaacaagag ttactgagat ccgtgataaa ggtcgagaaa tagtattatc agatggctcc
    51661 gtcctttctg ctgatatgct aatttttgct gttggtgttt ctccgaagaa tgaagtggtg
    51721 aaagcaaccg gcatacaatt atcagataca ggtcagatca ttgttgatga ccagttacaa
    51781 accaatctgc cggacatcta tgcgattggc gatattatcg aaacgactag tgtagtgact
    51841 ggtcagccga tccaaagtat gctttccagt gcggccaatc gtcaaggaca tatgctggca
    51901 gatattttaa atggtacgcc tatgcgctat cgcggatata ttggtgcagg tgtcgcaaaa
    51961 atctttgatc atacagcaag ttatgcggga atgacagaac atgaattaaa agcatcaggc
    52021 ataacaaatt ataaaactgt ttttatcact ccctttgacc atgcctattt ctatccagaa
    52081 gctacaagat taaatctaaa gctgattttt gatgcagata gcggtcgtat tttaggtgga
    52141 caagcatttg gagaaaaagg tgtcgataaa cggatgggag aactttctgt agcaatcacc
    52201 ggaaacttga cagtctttga tttgcctgat ttggagttgc cttactctcc accgtattct
    52261 actacccgtg acccgttgaa tatagctggt tatgtcgcaa tcaatcaaat gacgaatatc
    52321 gtagaaacga tcaaagcaag tgatataccc gaaaacgatt tgaaagaagc gttcttttta
    52381 gatatacgtg aacctaataa agcaccttct ggcagtatct cggcaacaaa aaatattcca
    52441 atgaatgagt tacgagatcg aatcaatgaa atcccaaaag ataaaaaagt ttacattact
    52501 ttcagaagag gattgaatac ttatacttct gcccgaatct tggcaggttt gggtatcaaa
    52561 gcgattttga ttgaagaata aaaaaacgac cgcggtgggt agccgcggtc taggagttat
    52621 ttttacttga ggagtaaaaa tgaaaaagta tatagggttg ttggttggta tgtatataga
    52681 atagaagata aatgtgaaga aattgtgcat tatttcttca catttattag gttatagttt
    52741 aagaaagttt aaacattgcg caatttaatt attttagctg tttcttttca taaaaaaaca
    52801 tgttgtgact ctgagacaaa tcatagtcca tagtgatgtc tctagagtaa ctacgcctaa
    52861 tattacgagg catcatatta atctatcagc actagtacct aaactagcat ccctttcgaa
    52921 tattttttct tatataataa agtttgcttt cactcctcta atctcaccaa atcgactggc
    52981 ttgcgttgtg tgatactagg aactgattgg tttagaacga cttttctatt gtaatcatat
    53041 ttttcgccat cctcgttgtc ttctaatcct tcaataaatg atccctaatg tgtgtattaa
    53101 ataatttaat ttttttgttc ctatttattt a
//
LOCUS       DACDYM010000010        50718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDYM010000010 DACDYM010000000
VERSION     DACDYM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.010"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..189
                     /locus_tag="IWM59_000626"
     CDS             <1..189
                     /locus_tag="IWM59_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9978682.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="IWM59_000627"
     CDS             complement(1055..1585)
                     /locus_tag="IWM59_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP9978683.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="IWM59_000628"
     CDS             complement(1582..2199)
                     /locus_tag="IWM59_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9978684.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2781..3002)
                     /locus_tag="IWM59_000629"
     CDS             complement(2781..3002)
                     /locus_tag="IWM59_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP9978685.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3180..4712)
                     /locus_tag="IWM59_000630"
     CDS             complement(3180..4712)
                     /locus_tag="IWM59_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9978686.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="IWM59_000631"
     CDS             complement(4848..5933)
                     /locus_tag="IWM59_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9978687.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="IWM59_000632"
     CDS             complement(5948..6667)
                     /locus_tag="IWM59_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9978688.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="IWM59_000633"
     CDS             complement(6664..7974)
                     /locus_tag="IWM59_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9978689.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="IWM59_000634"
     CDS             complement(8001..8591)
                     /locus_tag="IWM59_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAP9978690.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="IWM59_000635"
     CDS             complement(8909..9970)
                     /locus_tag="IWM59_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9978691.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="IWM59_000636"
     CDS             complement(10538..11605)
                     /locus_tag="IWM59_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9978692.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="IWM59_000637"
     CDS             complement(11625..12104)
                     /locus_tag="IWM59_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAP9978693.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="IWM59_000638"
     CDS             complement(12121..12582)
                     /locus_tag="IWM59_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9978694.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="IWM59_000639"
     CDS             complement(12596..13846)
                     /locus_tag="IWM59_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP9978695.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="IWM59_000640"
     CDS             complement(14224..15675)
                     /locus_tag="IWM59_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9978696.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="IWM59_000641"
     CDS             complement(15996..16760)
                     /locus_tag="IWM59_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9978697.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="IWM59_000642"
     CDS             complement(16788..17486)
                     /locus_tag="IWM59_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9978698.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="IWM59_000643"
     CDS             complement(17498..18277)
                     /locus_tag="IWM59_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9978699.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="IWM59_000644"
     CDS             complement(18293..19237)
                     /locus_tag="IWM59_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9978700.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="IWM59_000645"
     CDS             complement(19283..20062)
                     /locus_tag="IWM59_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978701.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20442..22517)
                     /locus_tag="IWM59_000646"
     CDS             complement(20442..22517)
                     /locus_tag="IWM59_000646"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9978702.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IWM59_000647"
     CDS             complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IWM59_000647"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9978703.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IWM59_000648"
     CDS             complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IWM59_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9978704.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24783..25682)
                     /locus_tag="IWM59_000649"
     CDS             complement(24783..25682)
                     /locus_tag="IWM59_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9978705.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25697..26098)
                     /locus_tag="IWM59_000650"
     CDS             complement(25697..26098)
                     /locus_tag="IWM59_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9978706.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IWM59_000651"
     CDS             complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IWM59_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9978707.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26569..28761)
                     /locus_tag="IWM59_000652"
     CDS             complement(26569..28761)
                     /locus_tag="IWM59_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9978708.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28785..29756)
                     /locus_tag="IWM59_000653"
     CDS             complement(28785..29756)
                     /locus_tag="IWM59_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9978709.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30462..31841
                     /gene="brnQ"
                     /locus_tag="IWM59_000654"
     CDS             30462..31841
                     /gene="brnQ"
                     /locus_tag="IWM59_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9978710.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(31997..32443)
                     /locus_tag="IWM59_000655"
     CDS             complement(31997..32443)
                     /locus_tag="IWM59_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9978711.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(32470..32646)
                     /locus_tag="IWM59_000656"
     CDS             complement(32470..32646)
                     /locus_tag="IWM59_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9978712.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(32808..33236)
                     /locus_tag="IWM59_000657"
     CDS             complement(32808..33236)
                     /locus_tag="IWM59_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9978713.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            33328..34188
                     /locus_tag="IWM59_000658"
     CDS             33328..34188
                     /locus_tag="IWM59_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9978714.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(34282..34575)
                     /locus_tag="IWM59_000659"
     CDS             complement(34282..34575)
                     /locus_tag="IWM59_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9978715.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(34648..36375)
                     /locus_tag="IWM59_000660"
     CDS             complement(34648..36375)
                     /locus_tag="IWM59_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9978716.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(36589..37515)
                     /gene="trxB"
                     /locus_tag="IWM59_000661"
     CDS             complement(36589..37515)
                     /gene="trxB"
                     /locus_tag="IWM59_000661"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9978717.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(37660..39093)
                     /gene="ascB"
                     /locus_tag="IWM59_000662"
     CDS             complement(37660..39093)
                     /gene="ascB"
                     /locus_tag="IWM59_000662"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9978718.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(39093..40496)
                     /locus_tag="IWM59_000663"
     CDS             complement(39093..40496)
                     /locus_tag="IWM59_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9978719.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            40726..41628
                     /locus_tag="IWM59_000664"
     CDS             40726..41628
                     /locus_tag="IWM59_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9978720.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(41669..43555)
                     /locus_tag="IWM59_000665"
     CDS             complement(41669..43555)
                     /locus_tag="IWM59_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9978721.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(43652..44503)
                     /locus_tag="IWM59_000666"
     CDS             complement(43652..44503)
                     /locus_tag="IWM59_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9978722.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(44838..46622)
                     /locus_tag="IWM59_000667"
     CDS             complement(44838..46622)
                     /locus_tag="IWM59_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978723.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(46622..48373)
                     /locus_tag="IWM59_000668"
     CDS             complement(46622..48373)
                     /locus_tag="IWM59_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9978724.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(48515..48739)
                     /locus_tag="IWM59_000669"
     CDS             complement(48515..48739)
                     /locus_tag="IWM59_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9978725.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            48990..50576
                     /locus_tag="IWM59_000670"
     CDS             48990..50576
                     /locus_tag="IWM59_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9978726.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT        14903 a        10618 c         8065 g        17132 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatcttttt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttgatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac gacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt
    17401 tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct
    17461 gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc
    17521 gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt ccttttttac
    17581 ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcaaaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa
    19321 cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc tgctctattt
    19381 gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga ccgtctttca
    19441 gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta tcagcgaatc
    19501 ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc atattttcac
    19561 gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca tccacagctt
    19621 gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg atcttcagtt
    19681 ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag
    19741 cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt ttgatagaag
    19801 atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt aggaaaattt
    19861 tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg gtgtcttttt
    19921 gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc tgattattga
    19981 gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca gtagccaata
    20041 acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga taagtaaaga
    20101 tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt aaactaatat
    20161 attcatagaa atatttataa acaggttttc cctatataat cataccacat acagtttttt
    20221 tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatta taattaatcg
    20281 ttttcatttt tatttcctca cttttcatga gcgttttgaa aattcgaaca aagaataaaa
    20341 taacaaaaat tattaaaata gaattaataa cagctattta taacaaaaaa aacagagaca
    20401 cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt gatgatttga
    20461 tccaagcttg ctaatgacaa agccatggca gctatttgac ttagttgtgt tagtcggttc
    20521 ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt ttcgatcagt
    20581 ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct
    20641 ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg
    20701 tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac acgtgtcaaa
    20761 gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct
    20821 gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat gacatcatga
    20881 cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacttgt
    20941 tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat aacatcgcta
    21001 atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat gatccgaacg
    21061 acaccgtatg cttggcgacg taaggcataa ggatcgtttg acccgcttgg aatcatccca
    21121 actgcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac agaacctact
    21181 gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg aatcgctgtc
    21241 gctacagcag gtttctcgcc tttcagaaga gcatattttt cgcccatgat cccttgtagt
    21301 tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc agcagctcgt
    21361 tgcaaatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat acgggcaatg
    21421 acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa agtaacagtt
    21481 tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc
    21541 gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatc ttccaatttt
    21601 acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg atcattccgt
    21661 acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa
    21721 agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa attgttgact
    21781 tcttctagta aatcttcgtc tagatcaata acccagtgat ttttttctgc taattcgttt
    21841 gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa ttgttcattc
    21901 aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga
    21961 cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac ttcgtcatct
    22021 aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc
    22081 atcgttacag ggaaagtcaa acttttgatc acccctgcta agcctgtcaa tacttctttt
    22141 gcttccttac catgagtgta tttcgttaca tagacatatt ccacaccttt taattctcta
    22201 aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc tgctttcgtc
    22261 cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg
    22321 tccgcttgtc gttctggaat atttgttact ttgattgcta aacggcgcgg tgtggaaaat
    22381 gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct
    22441 aattgtttca tgcttggtgt gacaacatgg gcaggcattt cttccagccc gatttcaagt
    22501 aataagtctt ttgccatctt attcagcctc cttcacggtt tctggatttg ctgtgttttt
    22561 attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg
    22621 agccatgttg cggatgcgag aaagataacc ggcacgctct gttacggata ctgcaccgcg
    22681 ggcgtctaat aagttgaatg tatggctaca tttcaagata taatcatacg ccggatgaac
    22741 aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag
    22801 caagtcttgg tcgcttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa
    22861 gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt caacttcttg
    22921 gatataagaa gcaagacgtt ctaatccata agtgatctca gaagttactg gtttacaagg
    22981 cagtccacca acttgctgga agtacgtgaa ttgtgtgatt tccattccgt caagccatac
    23041 ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt caacaaaacg
    23101 gatgtcgtgt tctaatggat cgattcctaa aaggcgcaag ctttctaaat aaagctcttg
    23161 aatattctca ggtgaaggtt tcatcaccac ttggaattga tgatgctgat acaaacggtt
    23221 tgggttttca ccataacgtc catcagctgg tcggcgagat ggctctacgt aagcagcatt
    23281 ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt
    23341 ttctgtgtca taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt
    23401 taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttaa
    23461 agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct atgtctgatt
    23521 acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag attcggcagc
    23581 ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc
    23641 ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc
    23701 agttttcttg tttctataat agatcctatc actgaacgat ctatttaatc tatggttaat
    23761 aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta tgttttattt
    23821 actattcttc tttttcaggt gtctcttttt ctttttcaga catgtctttc tctgacgtct
    23881 ttaatacgtg tgcccaagac ttcatttgat tgatgaactt tttgcttttc aaatggatac
    23941 cgacatattc atcatacagc tggtcgatga gttcacggat tgcttgtttg gtttcttctt
    24001 tcagatggat tccactaatt ttatcataag aaatagctga aaacatccga acgaaataaa
    24061 cagctcgagg atctgcatga taacggtgat cgtccatcga ccaatgtctt tcgcatatca
    24121 caccaccata tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacgcagt
    24181 gtgtccagat tggtgcaatt ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga
    24241 tgatttctgc atcgtctcct tggtccatcc gttgcagtgc ttgttgcgta aactggaaca
    24301 aatgcgggtc gtactgatga tcttcgattg ccgcatctac tagattcaat aaatatgtgc
    24361 catagccgct caaaaagatg tcttcttgga tgtgtcgaaa cgcatggaca tctttgacac
    24421 tgcttaaaaa tgacaaacct tcacttcgaa aattcccgat ataaactgct tcagtaaaag
    24481 ggagcaaagc cggagctaag ggctgatttt gccgatggat ccctttgata taaaacatca
    24541 ttttgccaga agattccgta aagattttga ctaatttatc tttttctttg taattacggc
    24601 tagataaaat caagcctttt gattctgcaa gctggctcat attctccctc ctaaaaaata
    24661 aatctggaaa agcagattct gccgatccag atcatttatg ttgcgatctt ccgcagcttt
    24721 tctcaacacg cggattttta tccagtttat ttctatacct aacttacaag aagctcagaa
    24781 tatcaatatt cgtcttttcg gtagccgaag tcttgcagat agactttttt gtctcgccag
    24841 tctttttgaa ctttgaccca aagttctaga aagactttgt ctcctaaaag attttcgata
    24901 tcttttctcg ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatc
    24961 cctttttggc tatctctttc gacaataatc gtagcctgga tatggatttt atcattctcg
    25021 tttcgcttca tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa
    25081 aacaagactt tctcacggat caattcagaa acgatgaagt attcaggatg atcagtcact
    25141 tgatcatctg gaaaatattg tgggccctct ggcatttcac tgactaatac atccatcaaa
    25201 cgttcgacgt tgtttccttc tgtagcagaa attggaatca cttgtgcaaa cggcatctgc
    25261 gttgaataat cttcgatgat tcccaacaac tcgtctggat ggattttatc gattttattg
    25321 ataatcagat aaacaggaga atgcacgttt tttagacgtt caataataaa atcgtctccc
    25381 cgaccgcgtt tttgatcagc gctgatcata aataagaccg cgtccacttc tttcaacgca
    25441 ctgtaagctg cttctaccat aaaatctcct aaacgatgct ttggtttatg gattcctggt
    25501 gtgtcaataa aaatcaactg agcttctggt gtagtatata ctccttggat tttatttcta
    25561 gttgtctgcg ccttatcact catgatggca attttttgac ccacgattcg attcaataat
    25621 gtggattttc ctacattcgg gcggccgacg attgcgacaa accctgattt atgttctggc
    25681 atatacgttc tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca
    25741 acccaacgat gacggaaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga
    25801 tttttttccc aatcggatga aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa
    25861 cagtattgat gatctctact atccatacga gaaaaacagc taaaagcagc cataaccatt
    25921 ctatccgatc aagctggaaa atcacgccca taaacaaagc gaggattccg aacgcaacat
    25981 gtttcctcat gttgcgttct tcctcaaaaa cggttttgac tccttggacg gcaaattcca
    26041 cggacatcag gaaatgtttg tttttctcta cgtttttatc ttttaagtcc ataggcatct
    26101 aagatctctt tctgtaaacc aaacatttct ttctcatcct ctggtgtcat atggtcatac
    26161 ccgttgatat gcaagaatcc atgaaccgct aaaaagccaa gttcccgatc atacgaatgg
    26221 ccgtaagctt ctgcttgctc tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca
    26281 cgaggcaatg gattctctcc atcttcaaaa atcactggga tttcgtcttc gccctcttct
    26341 tccaaggcaa aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt
    26401 acttggatcg ctgcattgtc catgaaagta acagacattt ctgtttcttc tggcaactct
    26461 aaatatgatg ctgcaaattg cagcaagtcg tcgatttctt tcattttctc ttctgagact
    26521 tggtttgttt catccatgaa agtaatatcc attatacatc gcttctttct attcttcttg
    26581 ttcttctttt atcttctcaa cttccgattt cattttagga tatttgatcc gagaatggaa
    26641 ggtactgctt aaaccgctga tcaatgattt ttctacttta cggatttctt ttaacgtcaa
    26701 tccactgtcg tccaattgtc catcggaaat acgctcttca ataagattat ggacaaaact
    26761 ttcgattttt tcgctcgttg gatggtccat tgctcgtact gccgcttcac aactgtcagc
    26821 tatattgacg attcctgctt cacggctttg aggttttggt ccaggataac ggaattgctc
    26881 ttccgtgact tccgggttgc gttcctttgc cttaacgtag aaataacgca tcagcgtggt
    26941 tccatgatgc tgtctgcaaa tatcgatcac catttgcggc atattataat cttccaatat
    27001 tttggcacca tctgtcacat gtccaaagat aatatgttta ctgtcttctg gcaataagaa
    27061 attgtgcgga ttttccgctc ctgaaggcaa gttctcaacg aaaaagctgg cgtgtttgat
    27121 cttgccgata tcatgataat agcaagctac tcgagttagt aaagaacgtc caccgatttc
    27181 tgctacagca ttagcactta gattggctac catcatgcta tggtgatacg tgcctggtgc
    27241 ttcttccagc aactgtttca ataaaggatg attaggattg ctgagttcat ttaatacgat
    27301 cacactatca tcattgacca tcaattcaat ataggggtgc agacctatcg tcagcaagaa
    27361 tgagaacagt gcacctgtga atccgcatac tagcgttaac caagtcgttg catccccaaa
    27421 gctcattcct tggtagataa tcaaaatgac atccatcatc acaggaaaaa taatgaccca
    27481 catagcagcg gataaccctt gttctgagat ccgtttgcgt ctgacgatcg ttgccatgag
    27541 accagaaaat acataggaca tcaaaataac tgtcagaaag ttcgtgccaa tcgaatcata
    27601 aaaaatgaaa agtgcggata ctgcttgaaa aataccagcc atgatccctg ctcttcgatt
    27661 caagaaaaag cttaaaacaa gtggagcaaa agccgcgggg taaaacagcg gaatataagc
    27721 agcttgttct gtttggaaga gttggaaaaa tttcattagt acgacgctta ctgacattgc
    27781 cgtcacgtag aaaagtacgt attttgttct ttctcctgca ttttggaatt gaagtgaatt
    27841 ataaatcagt acaccgattt gaagcaaaac agctaaaacc atggcaatga gtgggaaaat
    27901 agaagtcgtt tgattcgtca atcctagtaa ttccaatttt ttcatcgctg aagcatcaat
    27961 ttgatttcct tcgcgaacaa taatttctcc ttggaagatc atcactggtt gtacagcttc
    28021 tcgtgcagat tgttttaatt cttctgtctt tttctcattc aatacatcat taactacaat
    28081 gccttgatcc actaagtagc gaatcatttg ttgctgctct gttgtgacat tcaaatattg
    28141 aatctgatct tctgcttctt gtttaaaagc ttccaattcg ctttcacgaa cttgtttact
    28201 catctgttcg tcaatgagct tcaaactttc atccctaacc gtgtgaattt ccgtttcagt
    28261 catttcaaat atcgtcgaat aaaaattgtt cggcagtttt tggtaaaacg caacgttttc
    28321 tgcattcacg ttttcaaaat ttttcttcag tgtagcaatc cgctcttctg tagtaggttt
    28381 cgggatattt tcatcttcct tcgccttatc taccttgtca ttataagatt tgttgatggt
    28441 atcattcgtc ttatcgatca attcgaataa ttgcttgatc cgattgttct ggtcatctgc
    28501 cagatctttt tgataagtat attctggagt cactgcttct gctgccagtt ttcttctttg
    28561 ttcggtttct tccgtgttct caatcgtttt attcgcacgg atactctctt ctgctacttg
    28621 gccttctctg aaatcattgg ccttttgttt gacactacta aatgtaacgc cgcagacaat
    28681 gataaaaaac aagaacagaa ggaaaggcaa tgctgccttt ccgagttttt tttgtagttt
    28741 ctgcagtgtt ttattcaaca tgtgcttatc ctcctaagca aagcttattc ttgatgaaga
    28801 tccgcctttt cataagcttt gatgatttcg gcaactacag ggtgccgaac aacgtcactt
    28861 gcttcaaaat taacaaaatc gatttttttg atttgcttta atgttcgttc tgcatgaaca
    28921 agaccacttg tcgtcccacg aggcaagtct atctgacttg tatccccatt gacaatcatt
    28981 tttgattgat aaccgagacg ggtcaagaac attttcatct gagcgactgt cgtgttttgt
    29041 gcttcgtcca agataacgaa tgcatcatcc aatgtccgcc cccgcatgta agctaaaggt
    29101 gcgatttcga tcacgcctcg ttccatcaat cggttcgtat gatccaatcc aaaaatttgg
    29161 tataaagcat cataaacagg ccgcagataa ggatccactt tttctttcag atccccgggt
    29221 agaaagccta ggttttcccc tgcctccaca gcgggccgcg tcaaaatgat tttttgcact
    29281 tcgccttttt ttagggcagc aattgctaat actacggcaa gaaacgtttt ccctgttccg
    29341 gctggcccta caccaaatac gatgtcatgt ttggctaccg attcaacgta tttcttttga
    29401 ccgctgtttt ttacccgtat aggttttcca ttacggtctt tgacgatctc ttcctcatac
    29461 atctcaacaa aataatctaa tgtacctttt tgccccatct tcaatgccgt cacaacatct
    29521 ggcgtactta cgtggattcc tcgtttgatc aattcctgta atgtccgaat gactgaggca
    29581 gccaaggaaa tctgggattg ctcaccaatg atctgaatga tttctccacg tgtatggatg
    29641 gttgtttccg ttgtttcctc gatcagtttt atatgcttgt cgtgtgcacc taaaagcatg
    29701 ctgacatcat cagcagctgt caatctaatc tctaaagaac tagactcttc cgtcaaaaat
    29761 agacatcccc tttttttcga ttcattcttt cctattttac catactcaga agaaactata
    29821 tagcaaataa gaaaaggttg taaaagagtc gtttaaccat acgtaccaag aaacgttaaa
    29881 aaatagccgt ttccattcgc taaaggtctc gttttgataa aaagaagctg agaatgtaag
    29941 ttatatgaaa gtggagaaca aaatattccg tttcattcag caggtattct ctaggtattt
    30001 ttcttgtttc aaaaaaagaa aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca
    30061 ggtgtattat tacgtcaaca aataaacata cagcaaagcg tcgaggaaaa cagtaggcta
    30121 ttcaacgatt tattcccagt gagtcatgta agtgagaaat gacaataaat cgaatagacg
    30181 aacatcattt ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc cattatcagg
    30241 ctagcggata actttatcaa aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa
    30301 ggtggtaccg ttctagaatc tagagccctt ttcccaagtg gaaaagggct ttttttatat
    30361 tttaaggtgg tggttttcat ggaatctgat attagtggca ttactaattg ctgcaacgtt
    30421 tatatcgtca atcaaaatga taattattgg aggaaaaatt tatggaagaa acaaaaactt
    30481 attcatggaa acaactattg atggtcagtt cacttatctt cgggatgttc ttcgggtcag
    30541 gtaatctaat ctttcctgtc cacttaggtc aaatggctgg cgcaaactgg gtcagtgctg
    30601 gcgtaggatt tgccatctcg ggcgctcttt tcccattact agctatctta gctgtagtag
    30661 tgacaaaaag tgatggtctg tatgacttag ctaaacctgt cggaaaatgg tatgctgcct
    30721 tgttcttaat ccttgttcat ttgacgatcg gaccattttt cggtacacca cggacagctg
    30781 ctactgcttt tgaaatggct gctaaaccat ttttgcctga aaaatataat tcattaggta
    30841 tgtttttatt ctctgctctt ttctttttac ttgcttattt ccttactgtc catcccacac
    30901 ggttggtcaa atacgtcgga aagtatctaa actccttgtt tcttgcattg ttagcaatca
    30961 tttttgtcgt ttctttattc cacccaatgg gaaatctaaa tcaatctgct gcagatactt
    31021 accaaacaaa cgctttaaca aatggtgtcc tagaaggtta taacacggtt gatgcagtag
    31081 ccttgcttgc cttaagtgtc acttttgtcc atgcagtccg tgaccttggt ttcaaagata
    31141 aaaaagtcac agaattaaca gcaaaagctg gtagcctgag cgttgcgcta gaagtcatga
    31201 tctattttgg tcttgttctt ctcggtgcga tgagtttgaa taagatgtct ttatcagaaa
    31261 atggcggaac tgcactttct cagatcacta atttttattt aggtaacttc ggcgctagct
    31321 tcttaggagt gatggtcaca ttaggtgtct tcactaccgc tatgggactc gttgtttctt
    31381 ttgcgcaaga tttccataaa ctgttcccta aagtcagtta tatgacatgg ctgcggttaa
    31441 cgactttcgt ctcttttgtc gttgcaaatg ctggtttaga caatatcatc caatggtctt
    31501 taccagtatt gatgcttttg tatccattgt cgcttgcgtt gatcttgctt tccttaacag
    31561 caaaattttt ccaaaagaca ccatttgtct atcaagtaac gatgttgttt gctgctgttc
    31621 ctgccgtatt ggatatgctg gcaaattcgc ctgctttagt gagtcaacaa agagttgtag
    31681 caagcatgct cgaattttat catcatcacg ttccttttgc tgcattagga cttggctgga
    31741 tggtcccaac gttacttggt tacgctggca gcctactgtt ttattacgcc tatcgcctat
    31801 cagggtataa acaagaagca aatgaactac cagaagaata accaaatcaa acacaattta
    31861 acataagaaa tattaaacag gaaaaaacca gctagatgaa agaatcctat tctttcgtct
    31921 agctattttt ttgttctctt ttttacgcgc acaaaaaaac tgcggacaat gattgtccgc
    31981 agtttaaatg ataaaattaa gattgtaata gttctttcac aatggcattt acttggtttc
    32041 catctgcttt tcctttgact ttaggcatga tcgcacccat gactttgcca aattctttca
    32101 ttgatgaagc accagtttgt gtgattgctt cttgaacaag ttgtcgaatt tcttcgtcgg
    32161 aaagttgttc cggcatgtat ttttcgacta tcacaatttc actttttact ttttcagcta
    32221 aatcgtcacg tcctgcttct tcaaactcat gcaaagaatc acgtcgctgt ttcatttcgc
    32281 gagacaacac cgttaattct tcttcttctg tcaagtcgtg accttcttta atctgctcgt
    32341 tttgtataga tgacttgatc attcggatga cttgtaaaga ttccttatct ttcgctttca
    32401 tcgctgtttt catgtcatcg tttaatctac ttagtagtga cacactacca actccaattc
    32461 atcacagaac tagaattttt tgcgttttct agctgcttca gattttttct tgcgtcttac
    32521 acttggtttt tcgtagaatt cacgtttacg agattcttgt aaagtacccg cttttgacac
    32581 ggaacgtttg aagcgacgaa gagcatcgtc aagtgattcg tttttgcgaa cgactgtttt
    32641 tgacatgtta attccctccc tccgagctta aaaagtaacc gtaattgtta ttgaaatata
    32701 tctcaaaaaa caagactgtc ctgtaaatat tatatctaat gctgttttga ccgtcaagga
    32761 aatcttgctt ttttttaaaa ttatcaaatg ttattttatt taaatcctta ttgacaattt
    32821 tcacatcttc cgaaaatctc gaatctatgc ccttcgatcg tacatccacc gagctgttct
    32881 tcgaaataat tcattggaca catatggatt tctttcgttg ttccgcacac cgtacagata
    32941 aagtgatggt gatggccgat ttccccttga cagcaatgaa aacgaaactt catctctcca
    33001 tttaaatcgg tttcttccag taaatcgatt tctgcaaaat catggagatt tcgataaatc
    33061 gtatcgtaac ttactccttt gaattcctga ttcatatagt caaaaacttc tttagcggat
    33121 acgtagcgat tgcgcttcac taaataagtt aacaatgctt ctcttttttt agtatattta
    33181 aaaccgttat ttttcaatgt ttctagtgat tgttcaatca atgtctgttt tttcatgact
    33241 tattcctcca aatcaaaatg attacgaagt acacattcta gagtatggta taataaacat
    33301 aaaaaaaatg caagtgaggt tttatccatg tcttccgatt ctacagaaat cgttactttt
    33361 tttgtcatct ctgactctgc tggagaaact gcgacaaaat tagcgcaagc cactatggcc
    33421 caatatccta ccgttgagtt caatttattc cgcagaacat ttgtgacaga tgagaaaaca
    33481 ttgatgcagg cactgcaaga tgcactaaaa gaaaaagccc ttgtcctgca cacattgatc
    33541 aatcaagaat tgatcgagat gacgcgttct ttttgtgaag aacatcaatt atttagtttt
    33601 gatgtgatga ctccgccgat atcagaaatc gagcgcttaa ctggtatcaa gcccattcgg
    33661 caacctggcg cccttcattt attgaatgag aactatttca aacggatcaa agcaatggaa
    33721 tttgctgtaa aatacgatga cgggaaagat cctcgtgggt ttctagaagc agatgtcgtt
    33781 ttgctaggcg tatccagaac ttccaaaacg cctttgagct tgtttttagc taacaaaaat
    33841 ttaaaggttg ccaatctacc tttgatccca caagcacata ttcccaaaca gctttgggag
    33901 atcgatccta aaaaaatcgt gggactgacg aataacccag atattttaaa caatatccgt
    33961 aaagaacgga tgcgctctta cggattaaat cctgatactg cttattccga tatcgaaaaa
    34021 atacgagctg aactcgattt tgcgaatgat ctttatgaaa aattgggctg cgtagtaatc
    34081 aatgtcgctt ctttatctat cgaagaaaca gcttcactta ttttgaatgc tttagaccta
    34141 gaagatcata gttattatgg aactgaaacc accgaagaaa cagactaaca gtttttcttg
    34201 tctttttctt aaaaaaactg gactgtgtca gcgaagaaga ttcgttgata cagtccagtt
    34261 tgtctctatt tgcacctatc gttattgatg ttccacatgt ttgagcgtct ggttcaaaat
    34321 atccagtacg tgagaatcat caagactata caaaatcgct tttccctcac gatgggactt
    34381 gacgacatgg ttttctctta ataaacgcag ttgatgcgat acagcagatt gttccatttc
    34441 tactgcttgg gtgattgctg tcacattttg ttccccgtct ttcagataca gcagaatttt
    34501 gagacgagtg gggtcactca acattttgaa tagttggctg actttctcta cttttgtttt
    34561 ttgcgtgtct ggcaatccat tcacctccaa cttgaaaaaa gactgacttc taaagtttac
    34621 cactttttcc atcagtctca agttttttta ttcttctgtc atttttttga taatcgcttc
    34681 ataagcggct actttttcag ctgcgtcttc tgaagagttt ccttttacac cgatataaaa
    34741 tttgattttc ggctcagttc ctgaaggacg gatagcgatc cagccatttt cttccagcgt
    34801 atacttcaat acgtcagatg gcggtgtcgt tagtttttct acgtttcctt cacggtctgt
    34861 tctcgtcaac tctttgaagt cttctgtttg gatgactttt gctccggcaa attctgtcgg
    34921 tgcttcttca cggaattttt ccatcaaagc agagattttt gcagaacctt cttgtccgct
    34981 cattgtcaca gaaatcgttt tttctgcgta gtagccatat gtttcaaaaa tcgtttgcaa
    35041 gccgtcatac aacgttttgc cttgtttttt atagtaagct gctacttctg ctaataagag
    35101 caatgcttga atcgcgtctt tgtcgcgaac gaaaggttta actaaataac cgtaactttc
    35161 ttcgaatccg aacataaaag tatgggaatg atcttcttca aattgttgaa ttttttcagc
    35221 gatgaatttg aatcctgtca atacattgaa catggtagcc ccatagtgtt ccgcaattgc
    35281 tgtaggcaat tcgctagaaa cgattgattt caataccgct gcattttcag gtaaagtgcc
    35341 tcgcgattgg tgtgcttcca aaatatattg agcaaacagg gctccgattt ggttccctgt
    35401 caatacttca taggagccat ttggcaaacg aacggctgcg cctaaacggt ctgcatcagg
    35461 atctgttgca atcagtaagt cagcgccttc tttctcgcca agtcgcatcg catactcgaa
    35521 agcagagtgt tcttctggat tcggtgattt gactgtagtg aaatccgggt ctgcgacagc
    35581 ttgttctgga actagtacga attgttcgaa tccagcttgt ttcaacgcac gttcgcccaa
    35641 catttttcct gttccgtgaa gtggtgtgta cacaagtttc aaatctttac ccattgtttc
    35701 caccaattca tgatcaatcg ttactgcttt gatttcttct agataagcgg catccacttc
    35761 ttctccgatg atcgtgatca atccgctatg ttcagcttcg tcgtcagata atacttcaac
    35821 ttttaacgga ttgctgactt cacggacata tttcgtcaat gcatctgcgt ctgcaggagg
    35881 catttgtccc ccatcttctc cgtagacttt atatccgttg taagctgctg gattgtggga
    35941 tgcagttacc atgattcccg caaacgtctt caaatgacgg actgcaaaag aaagctctgg
    36001 tgttggacgt aaactttcga atacataaga agggatattg tgatgtgcta atgttttggc
    36061 tgcttccatg gcaaattctg gtgattgatg gcgtgagtca taagcgatag cgacacctcg
    36121 gcgttttgtt tcttcacctt gtgtatccat gaaacgtgca agtccttctg ttgcttgtct
    36181 taccgtatag atgttcattc gattgatccc aggtcctaag attccccgca tgcctgcagt
    36241 accgaattcc aacggtgcat aaaaagcatc ttcaagtttt tcaggatctt cacccagatc
    36301 aattaattgt ttttttaagt tttcttctaa attttcttca tgtacccatt gttcatacac
    36361 ttgttcccaa gacatgaaaa taacacctct ttctgaaata tggttcttaa taagtatagc
    36421 attcacttat tagatatgaa agtacgaggt ctaaaaaacg cttaaatcaa gaaaggattc
    36481 ttttatcaag cacggttgca agtattcgca taaatcggat tcagtagatt tcacgcgaaa
    36541 aaaaagagta aaagaacaat tgaaaccgtt cttccactct ttttaaactt atgcagttgt
    36601 ttcttctaaa gattccaagt atttatacac ttgttgtcca gcgatcccac catcaccaac
    36661 agcagtagtg atttgtcgca aatctttttc acgaacatct ccgatggcaa aaacgcctgg
    36721 tatattcgtc ttcatttcgt tgtctgttgg aatccagcca gcttcgttcg tcaaccctgc
    36781 ttctctaaat ggtgcagtta atggatccag tccaacatag atgaatactc cgtctgctgc
    36841 caaatcaaac tcattgccgg ttttgacatt acgtgctttc acacctgtca ctaccatttc
    36901 attcccaacg atttcgtcaa cgactgtatc ccacataaag gaaatttttt cattcgcaaa
    36961 tgcacggtct tggatgatct tttgtgcacg taattcatct ctacggtgga cgatgactac
    37021 ttcagaagca aattgtgtca aatagatggc ttcttctact gctgagtctc ctcctccgat
    37081 cactactaga cgtttgttac ggaaaaacgc accatcacaa actgcacagt aagaaacgcc
    37141 acgtccagca aattcttcct cgccaggtac gcctaattta cggtggatac agcctgttgc
    37201 gataatgact gtttttcctt cgtaagtttt atcttcacaa ataacttctt tgtggctgcc
    37261 gtgatccttg atatcttgca cgattccata agcattttct gtcccaaatc ttgaaacacc
    37321 ttcatacatc ttataagcta gatcaggacc catgattgaa tcgaaaccag gatagttttc
    37381 gatttctgcc gtattgttca tttggccgcc aggggcgcct tgttcgatca tcaatacgga
    37441 taagtttgaa cgtgaagcat ataaagcagc agtcatccca gcaggacctg cgccaataac
    37501 gattacatca tacatatgta gattctcctt tacttaatca agtaagcata ctttacagcc
    37561 aatgcgaaaa aaagtctatt gttctgcttg cgtttctcga atagctttat tatactcgaa
    37621 aaatccgctg aaaaacaata gacttttaca aatcattgat caatcattcg aaagattttc
    37681 tccatttgaa tcaattactt ttttatacca gttgaatgat ttcttcttcg aacgggctaa
    37741 tgtgccattt ccttcgttgt cacgatcgac ataaatgaat ccgtaccttt tcttcatctc
    37801 acctgtacca gctgatacta aatcaataca tccccaagtc gtatagccta atagatcgac
    37861 tccatctaat tctactgtat ctttcattgc ctggatatgt gctgccagat agtcgattcg
    37921 atagtcatct tccacataac cattctcatc gggcgtatcg actgcaccta agccgttttc
    37981 tacaataaat agtggttttt gataacgatc ataaatatca ttcatcgtta ttctcaagcc
    38041 taatggatca atttgccaac cccattcact tgcttccaga tatggatttt tgacagaagc
    38101 aaagatattt cctgcagtct gttccaagag ttcgttgtca gtagtcgaaa ctcgagaaga
    38161 ataataagaa aaggagataa aatcaacagt atgttctttc agcaatgcgg catccccagg
    38221 ttccattttg atctgaatgc catttcgttc catttctttc aatgcataag cgggatattc
    38281 tcctcgtgac tggacatcaa tgaagaaata attttctcga tctgattttc tagaagccca
    38341 aacatcttca ggattgcaag tgtatggata atatgctcct gctgccagca tacagcctac
    38401 tttgttctcc ggatctactt catgagcaat cttcgttgcg atggcacttg caaccaactc
    38461 atgatgtgcc gcttgatatt tgatttgttc ttcattttct ccttcttcaa aatacagccc
    38521 tgcgcccatg aatggtgcat gaaggatcat attgatctca ttgaatgtca accaatattt
    38581 gacaagtcct ttatagcgat tgaagatgac tcggcacaga ttttcataaa aaccaacgag
    38641 ttctctagaa cgccacgcac catattcttt gatcaaatgc atcggacaat caaaatgtgt
    38701 aatcgtcaca agcggctcga tcccgtactt ttgacattct ttaaagacat cctcataaaa
    38761 tttcaaacca gattcatttg gctcattttc atcgcccatt ggaaaaatac gactccaagc
    38821 aatggacatc cgataagtct tgaaccccat ttctgcaaac aaagcaatat cttccttata
    38881 atgatgatac atatcgatcg caatttttgc tggataaaaa tgatctttat caaattcaaa
    38941 cattttcttt tttccagtaa tgacaggaaa acggtcttct ccgattggca caacatctac
    39001 gttcgctaac ccacgtccgc cttcatcata accaccctcg cattgattgg ctgctgtagc
    39061 accgccccat aaaaaatctt ttctgaatcc catcatactt cctccattac atgtgtattg
    39121 cttgacaatt ctcttgccag ttcatcttgg acttcgattc cttctgcttt taaccggctg
    39181 attgtttcct tgaattgcgg taaatactgc tgatgtgcaa taaacaactc atccatgatc
    39241 cgttttgctg tctttcctga tcgtataagc ggattgatcg taaacgcttg taaagctgtt
    39301 ccataatcac cagtcacggc tgcttcgatc gtcagcaatt ccatattctt cattacttgc
    39361 aaccagcctc tttcagcagc aggaagggaa ccaaacgcta catttcgtgc gccttgtgcg
    39421 ccaacatagg cagtgacttc tactacacaa tctgccggca gatcaggaac agctccatca
    39481 ttctttgtag acaccacgat ctgtgtttct ttatttccat agatcgaagc aatcgtttca
    39541 caggcagcat ctgaataata agcaccacct cgtttttgca attgttcagg tttatgatcc
    39601 aattctggat ctttgtataa ttcaaacagc tctgcttctg tttctttcac ttgttgcgct
    39661 cttgtaccaa tcgtttgata ttcttctaaa gcatgttcca acatttcttc ttcacggtag
    39721 taatagcggt ggtagccaca agggatcatg ttcatttgtt ttaattgttc tttgaaaaac
    39781 gggatgtcaa agatattttt cggcaatcca ttgtcttcat cgtataaacg atcaatcaaa
    39841 tctgctgtgc ggtcatttcc tttattatct gctactttgt gccaatggaa gtgatttaga
    39901 ccggcaaact tgtagatcag ctcttctttt ttcttgccaa gcattgctgg ttctgtcatc
    39961 gtcgccatta ctggcacatt gcataatccg atcactttat cccatttgcc atatctgata
    40021 actgcttctg tcaccatccc gcttgggttc gcgaaattga tcagccaagc atttggacat
    40081 aaacgcttca tgtcttcaac gattgccaaa accactggaa cggtacgaaa agctttgaat
    40141 atcccgccag caccatttgt ttcttgccca atcatcccat aataactagg gatccgctcg
    40201 tctttgattc gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa atccgcatct
    40261 ttcaatgctt cttcccgatc caaagttgca tagatcttga cttcatacgg agaagcatcc
    40321 cacatacgct gagacatctg actgacgatc tcaagctttt cttttccttc ttctacatct
    40381 acgagccaga tttctcgtat cggtaattcc tcatatcttt tgataaatcc ttccatcaac
    40441 tcgggggtgt aactgctgcc tccaccaatc gtagcgattt tgattccttt tgccatgttc
    40501 tcactccttt gctttttaca cttttattat cttttttgta aacaaaacaa acaatagaaa
    40561 ttcgtttctt tccagtaaca tttacaaaag ttagtgtttt ctactggatt tttgtaaata
    40621 tcttgtcaaa aaatgacttt attaagctat ataacataaa tccgtttaca aatcattttc
    40681 aaaaaagtta taatttacgt aaaaaaacta gaagaaggta tctacatgtt attacgtgaa
    40741 aaaatcaaag aaatctcctt ttctccttca gaaaatgaag tgattcagtt tctcttaagc
    40801 gattctccag cacttggcga aatgaccatc caagagattg ctgagcgatg ttatgtccac
    40861 ccgtccacac tcatccgtgt agcaaaaaaa atgggttttg atggctgggt cagttttcgt
    40921 aatgcttttt tagctgaaac agcctatctt gaagggaatt ttcaagacgt agatgcgaat
    40981 cttccttttt ccaccacaga cggtattatg acgatcgcaa ataaaatggc ttctttagaa
    41041 cagatgacga ttcaagatac actttctctc attcatcatg accaactgca aaaagccaaa
    41101 caactaatgc ttcaagcaaa aaaaatcgtg attttcgcca gtaactcgaa tactttgatt
    41161 tctcaagatt tcatgctgaa aatgaagcgc atcaaaatgg atgtcacgat tgtcaccact
    41221 tttggtgaat cgttctatga agcgtacaat tgcacgagtg atatgtgcgg gattctgatt
    41281 tcttatacgg gtgaaaacaa tgtgatcctt cgtaccgcag aaatattgaa acaaaatcat
    41341 gttccaattt tagcgttgac cagtatcggt gattcaacgc tatcctcaat gagtacagcc
    41401 gctttgcgac ttacgacacg cgaacgactg tattcaaaaa ttggtaattt tacgatcaat
    41461 acatcgattt gttatcttct agacgtgtta tatagttgtg tttttgcaga aaattatcaa
    41521 cagaatttag tccatctaat ttctgtagga caaaaatttg ataaacgccc tatcagctca
    41581 gccatcatga aagagccttt caattttgaa aataaagaag tggactaaaa agaatagtta
    41641 agtctattcc attctttcac ctaaccgttc aggtcagtgc ggtgataagt gcatcgtctg
    41701 gcccgaccgt ctccccttga ttttccaaaa tatccaaata atccatgtaa ttggtgacga
    41761 ttacaggtgt ttctaaactg tatccttcct tttcgatttt ctttttgtcg aagcggagca
    41821 acagatcgcc ttgtttcact ctttctcctt gctgcaccaa gctttcaaaa tatttccctt
    41881 ccaactgaac agtatccaat ccgacatgaa tcagcaattc cagacctgtt tcagaaacca
    41941 ttccgattgc atgtttcgtg gggaacaggg tcatgactgt tccgtcaaaa ggggcacgta
    42001 cttctccttt ttcagggtag atcaagattc ctcgacctag tgttccttgt gcaaaagctg
    42061 catcctttgc tgctttcagt ggagcgattt gtccttgtat cggtgttttt acaatttctt
    42121 tacctgatct tttctgtctg tcatctgttt cacctgcttc ctccttccaa aagaaaaaag
    42181 tcagcgcaaa ggataccacc atcgcaatgg cgatcccaat caatacatta atcacatctg
    42241 ttaaatcacc atctggtcta atatagttgg gaattgtaaa gatgcctagt ccaccttgat
    42301 tccaagaagt taaattcatt aacccactat acgcgccccc taacgcacca ccgacacaag
    42361 taaaaataaa tattttttta cgtggtaatg taaacccata aatagctggc tctgtaatcc
    42421 ccacaatacc agaaacaatg gctggtattg caattgcttt gtcttttgga tttctaagct
    42481 taaagtagag tgcggtaagt gctgcagttt gtccaaaaga cactactgca acaggtgtca
    42541 taatattttg ccaaccattc acagaaaatt gcatcaatcc cattggtacg attgcccaat
    42601 ggagaccaaa catcaccaga atttgccaga agaagcctac gattgctcca taaataactg
    42661 gcgaaagtgc taaaagtgtt tcaaatccag ccattaaaat atcagttgcg aatgtgaata
    42721 ctggtccaac gagtaaaaaa cctaaaggca ttgtaataag aacggtgaag aatggaacga
    42781 agaaattcgc gatcatctca ggtactaact ttttaattgg tttctgaatt tggctagcta
    42841 acaacacaat aaagataatg ggaatgacag aagaagcgta gttacgcgca atccagggaa
    42901 tgccgaatac tgtttgataa ataggtgctt caaaaatagt gccagaaaat atggtcgtta
    42961 gtggtgcttc tgctgcacca ctcaaagctt ccagcgacaa gctaggattc attaatgctg
    43021 cgccaagcaa catccctacg aattcattta ctttaaattt acgggcagct gttaaaccga
    43081 taataacagg cataaattgg aatacagaat caccgatatt atcaaacata acaaatgtgc
    43141 cagaaccgcg gtcaaacgcg cctaagaaga caaggaatgc tgtcagtcca cgaatcatcc
    43201 cgccagcagc taagatagca aggaatggtt ggaaacagcc cgatattcca tcaataagca
    43261 tatcaaacgg tctcattttt tctgttgaaa cagcctcatc aaaagtaagg tttcctgctt
    43321 ttataacctc ttcaaacacg actggcacat gattcccgat gacaacttga tactgccctc
    43381 ccgctcgcat taccgttaca ataccgtcca tatttttcaa gatctcatca tttgcttttt
    43441 cttcgttttt taatttgaaa cgaagccttg tgatacaatg tgtcaaacca ttgatatttt
    43501 ccttcccacc gacattttct acgatttttc tcgctaattc ttggtatttt cccatttcta
    43561 ttccctccaa tatattagtg gaaggtttag ccaactgaat gtcactaccc ttatttcata
    43621 cgttcttgtt aatttatcct ccttttttta tttatttaca acgattctca tgcactaaac
    43681 gcgcagtatg gatcgttaaa tacatttttt cgtccgtgga catctggtag ttgaacgtct
    43741 cggctaaaaa gtctgctatt ttctcaacgc aaccaaacgt atccttatat tttttttgaa
    43801 taacctgaaa caattcctca gaaacttctc ccacttctga tttttctcca tctaggacgc
    43861 gtgtcagaaa aaaacgtaag tgtgtgacga atcgaaaaaa atggactgaa tcttcatcaa
    43921 atgtaatctt atagtaatag cggacgatat ttagaatatc ctgtattgtt atagtaaaca
    43981 ggtaggtatt gttttgttgg ctttgaatgc cttgtgcatt gactaaatgc aatgcgataa
    44041 atccggcttc atcttctgat aacttgatgt ggtatttatc cgagattttc ttcactgttt
    44101 ttttcccgat ggcatactct ttaggaaata gttgtttgat atcatacagc aatacatttg
    44161 gtacaaatat tcccattttg tgacgttcga tagccgtata caaatgatcc gtcaatgcaa
    44221 tatacacaga atcattcaac tggacttgta gagcctcttt tgtatattca atgatttcac
    44281 aagaaatttc taagtactca agaggaatcg cttgtgccaa ttcttggaac tgttgtgaaa
    44341 gcccgtcatt tgtcaactga aattttttgt cgatgcatga gtcaaaaatc gggtcaccca
    44401 ctttcttttt gaatgccaaa ccttttccca tcacgatcat ttcctgattt ctttcatccc
    44461 tggttattac gacgttgtta ttcagtactt tctcaatcag catgttttgc tcctttccat
    44521 gatatatagg gctgtattca aaacaaaaag aaaaacccaa aaagccgtcc atggaaccat
    44581 gggagactca tcaggtttag cttgcatcag gcaatcacta tccaagttgt atcgttttct
    44641 ttatattatc gaaagcaaac gcttttgtca acgctttctt agtggctatt aggcactttt
    44701 gactagctgt cgtcgtcttt tttaaatcgg gtgactacgc cactaacgct cacagatgaa
    44761 gaatctggga agctctgttt tattttcact ctagcgctta ggacaaaatg ctactatgct
    44821 actgtcacaa cctcttttta cacaccactg ctttgcagtt tgtacatatc tgcatacaaa
    44881 ccattttgcg ctagaagtgt ttcatgattc ccacgctcta ctatatgacc tttgtctaaa
    44941 actaaaatca attctgcatc ccgaatcgtg gacagccgat gggcaatcgc aatcgttgtc
    45001 cgtccttgtc gcatttttgc caatccttct tggatcaatc cttcggtttc agtatcgata
    45061 tttgctgttg cttcatccaa tactagtatt ttaggatctg tcacgattgt tcgcgcaaaa
    45121 gtgatcagct gtcgttgtcc actagaatag ctggcaccgt gttcgatcac ttttgagtga
    45181 tatccttttg gtaattcatc gatgaacttg tttgcttgaa caaactcagc tgcatggcga
    45241 atctgttcgt ctgtgatagc tggattcatc atccggatat ttcctgcaat atctccataa
    45301 aacaagaagg catcttgcaa gaccaatccc attttcttac gtaactcttc cattggatat
    45361 tctcgaatgt catgatcatc aatcaagatt tggccttcat aaaattcgta aaaccgcatc
    45421 aatacgttga tgatcgagct ttttccgctt ccggtgtgcc ccactaatgc aacggtctca
    45481 cccggattag cgacaaaaga gatattgttc aatacattgt gctctccatc ataagaaaag
    45541 cttacattgc gaaactcgat cttacctgaa gtaatcgctt gatctgctcc aggattttgt
    45601 tgcggtgcat attcttctgt atccatgatt ttcaaaatac gactaccggc aaccatccca
    45661 tctgtaaagg tgctcaaaaa gtccatcatt tgagacattg gattaaaaaa tccctggaca
    45721 taggtcgtaa atgcatagat cagtccaact tctacgggag aatgcaacgc atcgatccca
    45781 aacaaagtca acgccaacgc aatcgccaat gcatagagaa aattgatgat cggacttaac
    45841 agaatcgaat tcgttttgat catcgcatag cgcgtgtcta aatactcgtt gttcacttgt
    45901 tcaaattctt tagctaaacg agcttcttgg cgaaactgct ggataatacg catgccggaa
    45961 atcgattcat tcaatttcgt gttcagctga cttaattttt cccgcatatt tcggtaaata
    46021 cgagaactga atttctggta ataccagatg acgattccta aaatcggcaa gaacaccaaa
    46081 gtccacaagg caatttctcc attgatttga aacatggcaa cgaaagaaga aacgactgca
    46141 aagatcccag tcaataccat caaaaagaca taccaaaatt caaatagcgt ttctgtgtca
    46201 ttcgtcactc gagaaacggt cgatcctgcc ggtgtctggt caaaataacg catacctagt
    46261 gtatgcagct tttcaaataa tcttacccga acatactgat aggtcttcaa tgaagccatg
    46321 gaatacaaat accattggaa aaaccaaatg atacttttga ctatgactcc aaacaagtac
    46381 acaccagcaa aaaacaaaat aacttgtgtc gtagctgact gtttagccag atgatcatcc
    46441 ataaaagttt ggatgatcct tggcaatagg acattgatca cagacagggc aaacgcaaat
    46501 aagatagcaa cgatgaacgt tcttctaaaa ggtttggcga acttcatcag tcgtttgatg
    46561 atcttcttct gttctttaag tggaatcgat tttgtccatt ccgattgata attttcatcc
    46621 atcaggcctc gcctccttct atcttcgctt ctaattgctg tttttcccac attcgttcat
    46681 accagccacc taatgcaagc agctcgtggt gggttcctcg ttcaatgatt ttcccttgat
    46741 cgacaacaat gatttctttt gcatgcatca cgctgcttaa acggtgagct gcaatgatgg
    46801 ttgttttctc ttttcttatt tcttttaaat tatgcaagat agcttcttct gttttggcat
    46861 ctactgcaga aagtgcatca tctaagatca gcaattcagg atttttgatc aatgctcgag
    46921 caatcgaaat cctttgtttc tgtccgccag ataaagagac tccacgttct ccaactaagg
    46981 tatcataacc ttttggcaat tccttgattt cattgttgat aaatgctaaa gaagccgcat
    47041 gctctacttc ttcttctgaa agttttggat tagaaaaacg gatattatct cgaatggtca
    47101 tcgaaaacag gaagtgatcc tgaggcacat aaccgattga tcctaataaa gcattcaatg
    47161 aatattcttt gatcgaacgt ccgccaaatt ggattttccc ttgataatca tcgtattcac
    47221 gcatcagtaa tttgatgatt gtcgtttttc ccgcaccagt tttcccaaca atacctaatg
    47281 tttctccttc ttggatcgca aatttgattt gttctaatgc tggtgattct tctttcggat
    47341 agttaaaaga ggaaatagct acattcagct cgccttttgc cggggtctgc actgcaccca
    47401 gttgttccac gatatgcgtc ttttcatgca aaagttcatt aacgcgatca taagaagcat
    47461 ttcctctttc caatacgttg aacaatcggc caatcgcaaa catcggccaa acaagcatac
    47521 cgatataact aataaatgag accatctgcc caatcgagat cgtgccttcc aagacatact
    47581 ttccacctaa aataattgtt gcaacataag acagaccgat aatcaaggta atgaatggat
    47641 caaataagga gtctaaaaaa ttgactcgtt tgtttttaat aattgcatct tcaatctttt
    47701 cttcaaaatc cgcgagatct tctttttctt gtccaaatgt tttaatgact ttgatcccag
    47761 tgatactttc ttgtgtctta tcattgatac tagaaaaagc ttcttgcgaa tcacgaaaag
    47821 catcgtggag ttttgacccc agcactcgtg aagtaacggc tagcaaaggc agaggaatca
    47881 gcgcaatcag tgttaagcgc caatcaataa acaaaatcat cgcaatgatc gttgttccgc
    47941 ccgtaatcac tgaatctgca aaggtcaaga ttccagctcc tgctacattc tggattgcat
    48001 tcaaatcatt cgttgcatga gccatcaaat ctccagtccg atatttttgg taaaagagat
    48061 tgtccatttc tgtaaaatgt ttgaatagct gctgccgaag ttctttctcc aaccgagctg
    48121 cacttcccca aatattcgtc cgccagatat aacgaaaaag atattgtaag aaagctgcaa
    48181 ggatcaatag taagatccac caagaaatga tctttattga taaattctta tcggcgatct
    48241 catcaatgat gatcccaatc acttttggag gaactaactg taccaaggcc actgcgaata
    48301 gggagaaaat cccaattaaa taatgtttct tttcctgacg aaaaaaccaa cctaattttc
    48361 taaaaatcga cattttactc ctcctttgtc tttatcaaga gcataggatt gtctgtcctt
    48421 gtcttttttg ctgaatagtc tgaatagtct aacaaattac gaaataaaaa acggactgaa
    48481 aaaatcagcc cgcaaaaagt ctttgggtca gaacctattt tttcccttgg ttcttcattt
    48541 gctgcatcat ctggcgaatt ttcttctcag atggtttttg ccccattgac atcatcattg
    48601 atcgcaacat gtcctcatta acgggaggat ttttttctaa atagtctttc atatatttac
    48661 gcgctaggaa aaagccacct actgcgccga ttagcaaagc aataatagca ataagtacga
    48721 caatacctgt tgataccata taaatctctc ctttctattt ccgaattgct ctcaatgatt
    48781 tattttacta aagtttattg aaaaaagaaa ggcgtattct ttagtttcat gcaaaaaata
    48841 gtggtttttt tagagaagaa caaaatttct ttcattaacc agcaagacac gccacgaaaa
    48901 aaaattaggc ataccgaatc tttttcttta attttttctt tctgaggtgt agaatgtaat
    48961 cgtagataaa taggaaaggt ggttttttct tgaataatca atccaacaaa caatttcata
    49021 agcatcgact tattgaacat acaaatggtt tatctttagc tgaagtaaat ggaacagtgg
    49081 atgtaccaaa gggaaaaagt ttttttaaaa cacttcttgc ttactctgga ccaggtgcat
    49141 tagtagcagt tggttatatg gatccaggta actggtccac ttcgatcact ggtggacaaa
    49201 atttccaata tttattgatg tcagttattt tgatgtctag tctaattgct atgcttttac
    49261 agtatatggc agcaaaatta ggaatcgtga cccaaatgga tttggcccaa gcaatccgag
    49321 cacggacaag taagacattg ggtgtcattt tatggatctt gactgaatta gccattatgg
    49381 caacagacat cgcagaagtc attggtgcag caatcgccct ctacttactt tttgatattc
    49441 cattggtaat tgccgtattt atcactgtgt tagatgtttt cgtcctttta ttgctgacaa
    49501 aagtcgggtt tcgtaaaatc gaagctttgg ttgtctgctt gattttcgtc attttattcg
    49561 ttttcgtcta tcaagttggc ttgtctaacc ctgattggca cagtttattt gctggcttta
    49621 tccctaacag tcatacattc tcagactcac ctgtcatagg tggtgaaact ccgctgactg
    49681 gagcgttagg gattattggt gcaacagtca tgccgcataa cttatatctt cattcagcag
    49741 tttctcaaac tcggaagatc gatcaccaag acgaagaaga tgtagcacaa gcggtacgct
    49801 tttctacttg ggactctaat atccagttgt caatggcttt tttagtcaat gcactgctat
    49861 tgatcatggg tgtcgctgta ttcaaaaccg gttctgtcca agatccttcc ttttttggtc
    49921 tgtatgaagc tttatctgat ccatcagcta tgagcaatgg tatcttgatt gctgtagcaa
    49981 aatctggtat cttgtctact ttatttgctg tagcacttct tgcttctggc cagaactcta
    50041 ctattactgg tacgttgacc ggtcaagtca tcatggaagg attcatccat atgcgtatcc
    50101 cgatttggct aagaagacta gtcacacgat tgctttctgt gattcctgta ttgatttgtg
    50161 ttttgtttac aagtacaaaa ggaacgatcg aagaacatac cgctttgaat aacttgatga
    50221 atgaatccca agtttttctg gcttttgcat tgccattctc gatgattcct ttactgatga
    50281 tgaccaatag ccaagcggag atggggagcc gattcaaaaa ttcatttgtt gtaaaacttc
    50341 ttggctggtt ctctgtgatt agtctgactt acttgaactt acgaggatta ccaggtcaaa
    50401 tcgaaagttt cttcggcgag caagccacct cagctcaact agcattggct gatcaaatcg
    50461 cctatgtaat cattgttctt gtccttctcc tgttggcatg gacgattggt gatttatata
    50521 aaggaaacaa aaaatatgaa cagcgtttgc aagaaacgat agaagaaatc gaatagaaac
    50581 tgattctttt acacaaagga ggttgtgaca gaagtgggca gcttcaagaa ataagaagga
    50641 atttaagaaa attgcttttc aaatttttgt gaaatttcgg cttatttccg aagaagttac
    50701 ttctggaaca ccgtgtat
//
LOCUS       DACDYM010000011        49016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDYM010000011 DACDYM010000000
VERSION     DACDYM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.011"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..591
                     /locus_tag="IWM59_000671"
     CDS             <1..591
                     /locus_tag="IWM59_000671"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="catalyzes the formation of 1-deoxy-D-xylulose
                     5-phosphate from pyruvate and D-glyceraldehyde
                     3-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9978727.1"
                     /translation="RTHQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPV
                     AIRIPTHGVEHGTAIANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIE
                     ATLINPGTITENDVETLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFG
                     AEKEFTDSVPVGQLYERYHLTPKQIVEDSLRVLNIQ"
     gene            675..1133
                     /locus_tag="IWM59_000672"
     CDS             675..1133
                     /locus_tag="IWM59_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9978728.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            1242..1925
                     /locus_tag="IWM59_000673"
     CDS             1242..1925
                     /locus_tag="IWM59_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9978729.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            2072..2686
                     /locus_tag="IWM59_000674"
     CDS             2072..2686
                     /locus_tag="IWM59_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9978730.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            2702..2908
                     /locus_tag="IWM59_000675"
     CDS             2702..2908
                     /locus_tag="IWM59_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978731.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            3447..4004
                     /locus_tag="IWM59_000676"
                     /pseudo
     CDS             3447..4004
                     /locus_tag="IWM59_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            4062..6101
                     /locus_tag="IWM59_000677"
     CDS             4062..6101
                     /locus_tag="IWM59_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9978732.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(6210..7361)
                     /locus_tag="IWM59_000678"
     CDS             complement(6210..7361)
                     /locus_tag="IWM59_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9978733.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(7515..8045)
                     /locus_tag="IWM59_000679"
     CDS             complement(7515..8045)
                     /locus_tag="IWM59_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978734.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(8144..8494)
                     /locus_tag="IWM59_000680"
     CDS             complement(8144..8494)
                     /locus_tag="IWM59_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9978735.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(8651..10180)
                     /gene="ahpF"
                     /locus_tag="IWM59_000681"
     CDS             complement(8651..10180)
                     /gene="ahpF"
                     /locus_tag="IWM59_000681"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9978736.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(10190..10753)
                     /gene="ahpC"
                     /locus_tag="IWM59_000682"
     CDS             complement(10190..10753)
                     /gene="ahpC"
                     /locus_tag="IWM59_000682"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9978737.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(10897..11559)
                     /locus_tag="IWM59_000683"
     CDS             complement(10897..11559)
                     /locus_tag="IWM59_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9978738.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            11743..13389
                     /locus_tag="IWM59_000684"
     CDS             11743..13389
                     /locus_tag="IWM59_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9978739.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            13380..14462
                     /gene="uxuA"
                     /locus_tag="IWM59_000685"
     CDS             13380..14462
                     /gene="uxuA"
                     /locus_tag="IWM59_000685"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9978740.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(14570..16123)
                     /locus_tag="IWM59_000686"
                     /pseudo
     CDS             complement(14570..16123)
                     /locus_tag="IWM59_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            16271..17412
                     /locus_tag="IWM59_000687"
                     /pseudo
     CDS             16271..17412
                     /locus_tag="IWM59_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(17538..18617)
                     /locus_tag="IWM59_000688"
     CDS             complement(17538..18617)
                     /locus_tag="IWM59_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9978741.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(18726..19166)
                     /locus_tag="IWM59_000689"
     CDS             complement(18726..19166)
                     /locus_tag="IWM59_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9978742.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(19223..19633)
                     /locus_tag="IWM59_000690"
     CDS             complement(19223..19633)
                     /locus_tag="IWM59_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9978743.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(19814..20248)
                     /locus_tag="IWM59_000691"
     CDS             complement(19814..20248)
                     /locus_tag="IWM59_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9978744.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(20348..21196)
                     /locus_tag="IWM59_000692"
     CDS             complement(20348..21196)
                     /locus_tag="IWM59_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9978745.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            21585..23351
                     /locus_tag="IWM59_000693"
     CDS             21585..23351
                     /locus_tag="IWM59_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9978746.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            23453..24712
                     /locus_tag="IWM59_000694"
     CDS             23453..24712
                     /locus_tag="IWM59_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9978747.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            24859..26160
                     /locus_tag="IWM59_000695"
     CDS             24859..26160
                     /locus_tag="IWM59_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9978748.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            26163..27014
                     /locus_tag="IWM59_000696"
     CDS             26163..27014
                     /locus_tag="IWM59_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9978749.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            27027..27842
                     /locus_tag="IWM59_000697"
     CDS             27027..27842
                     /locus_tag="IWM59_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9978750.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            27993..29159
                     /locus_tag="IWM59_000698"
     CDS             27993..29159
                     /locus_tag="IWM59_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9978751.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            29389..30036
                     /locus_tag="IWM59_000699"
     CDS             29389..30036
                     /locus_tag="IWM59_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9978752.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            30120..31064
                     /locus_tag="IWM59_000700"
     CDS             30120..31064
                     /locus_tag="IWM59_000700"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9978753.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            31199..32650
                     /locus_tag="IWM59_000701"
     CDS             31199..32650
                     /locus_tag="IWM59_000701"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9978754.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            32671..33507
                     /locus_tag="IWM59_000702"
     CDS             32671..33507
                     /locus_tag="IWM59_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9978755.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            33507..34166
                     /locus_tag="IWM59_000703"
     CDS             33507..34166
                     /locus_tag="IWM59_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9978756.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            34292..34981
                     /locus_tag="IWM59_000704"
     CDS             34292..34981
                     /locus_tag="IWM59_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978757.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            34987..35943
                     /locus_tag="IWM59_000705"
     CDS             34987..35943
                     /locus_tag="IWM59_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9978758.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            35987..36499
                     /locus_tag="IWM59_000706"
     CDS             35987..36499
                     /locus_tag="IWM59_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9978759.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(36755..37303)
                     /locus_tag="IWM59_000707"
     CDS             complement(36755..37303)
                     /locus_tag="IWM59_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9978760.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(37392..38573)
                     /locus_tag="IWM59_000708"
     CDS             complement(37392..38573)
                     /locus_tag="IWM59_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9978761.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(38809..40317)
                     /locus_tag="IWM59_000709"
     CDS             complement(38809..40317)
                     /locus_tag="IWM59_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9978762.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            40476..40667
                     /locus_tag="IWM59_000710"
     CDS             40476..40667
                     /locus_tag="IWM59_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978763.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            40870..41787
                     /locus_tag="IWM59_000711"
     CDS             40870..41787
                     /locus_tag="IWM59_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9978764.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            41780..42853
                     /locus_tag="IWM59_000712"
     CDS             41780..42853
                     /locus_tag="IWM59_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978765.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            43001..43519
                     /locus_tag="IWM59_000713"
     CDS             43001..43519
                     /locus_tag="IWM59_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978766.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            43500..43892
                     /locus_tag="IWM59_000714"
     CDS             43500..43892
                     /locus_tag="IWM59_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9978767.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            43968..44903
                     /locus_tag="IWM59_000715"
     CDS             43968..44903
                     /locus_tag="IWM59_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9978768.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            45036..45815
                     /locus_tag="IWM59_000716"
     CDS             45036..45815
                     /locus_tag="IWM59_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9978769.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            45808..46125
                     /locus_tag="IWM59_000717"
     CDS             45808..46125
                     /locus_tag="IWM59_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9978770.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            46369..47979
                     /locus_tag="IWM59_000718"
     CDS             46369..47979
                     /locus_tag="IWM59_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9978771.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            48136..48984
                     /locus_tag="IWM59_000719"
     CDS             48136..48984
                     /locus_tag="IWM59_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9978772.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        16365 a         8758 c         9459 g        14434 t
ORIGIN      
        1 cgaacacacc aaggaacatt tgacattcct tacttatcaa gtatcccgaa catcaaatat
       61 cttgctccag caacaaaaga ggatatgatc tctatgctag attgggcgtt cgaacaaaca
      121 gcgcagcctg tagcaatccg aattcctaca catggtgtag aacatggtac agctatcgca
      181 aatgattata gtattccagc ctatcaaaca gtaaataatg gaggaaaagt agcaatttta
      241 gctttaggcg gttttcttga attaggaaaa gaaacagtaa aagcttttag aaaaaaagga
      301 atcgaagcaa cgttgatcaa tccaggaacg atcacagaaa atgacgtaga aacattagat
      361 aaattaaaag aaacacacga attagtcatc acattagaag atggtagttt gtcaggagga
      421 tttggcgaaa atatcagtcg ttattatggt gattcttcaa tgaacgtatt aaattttggt
      481 gcagagaaag aattcacgga tagcgttcct gtagggcagc tttatgaacg ttatcattta
      541 acaccaaaac aaatcgttga agatagtctg cgtgtcttga atattcaata attcattatg
      601 ctcaccatcc agataattgt ttatactagt atctggatgg ttttttcatt ataaaaagca
      661 aaggggtgga tgttatgtat cagcaagtag aagaatatgt caaaaagttc caagaaaaat
      721 atcctagtta tcaatcagaa aaattaggtt tcttttcatt tggggataca gaaaaaatgg
      781 cagacgagtt gagcgaacta gttgtaaaaa agataaagac tggtacatca agtggttacg
      841 atctgtataa acaagagaat gagcctttac caaaagttgg tcaattagac gtggtcctga
      901 atggaaaaaa tgaaccagtt tgtatcatca aaaatataaa tgtgaccatc ttgcctttta
      961 gtgaagtcac gaaagagcat gcttggaaag aaggagaagg ggatcggaca ttagcttatt
     1021 ggcggaaagt ccatgctgag ttttttaaac atgcttataa agaagcgttg aacatagaat
     1081 ttacagaaga gaagctggtc gtatacgaag aatttgaagt aattttcttt tagtaagaaa
     1141 ggtctgctga aagaaatagc tcattattgt ctcctgaata gaaaagggtt acatttttat
     1201 atgaagctgt atttttatat aacattactg gaggagatat tgtggataca ctggaacaaa
     1261 tcaaaggcgg agtcgtcgtt tcttgtcaag ctttggaaaa cgagccgcta cacagttctt
     1321 ttattatggg gagaatggct ttggctgcaa aagaaggcgg agcagttggt attcgagcaa
     1381 atacgacaga agatatcaat gaaatcaagc gaatcgttga tttaccggta atagggatta
     1441 tcaaaagaga ttacgatggg tcagaagtgt ttattactgc gacagaaaaa gaagtggaag
     1501 aacttttgga gacgcaagca gaaatcattg cactggatgc aacttctcgt aaacgtccga
     1561 cagaaaaaac aactgctgaa cttgtccaaa tgattcacga acacggtcgg ttagctatgg
     1621 cagatatttc gacttttgag gaagcgcttc aagcgcaaga agatggtttt gatcttttgt
     1681 ctacgacttt agcaggctat acagaatatt cacgaaagac gcagacacct gattttgatt
     1741 tattgaatca aatcatagaa caagtcgata tcccagtaat tatggaagga catactgact
     1801 ctcctgaaca agtagagaaa gcatatgaat taggtgcttt ttcagtcgta gttggatcta
     1861 ttatcacccg tccgcaattg atcactaaaa gatatgtcga agccgcaagt aaagcaaata
     1921 actaaaaaac acgcacggca cagattcttg acaaaaaaga aaaattgata gatagtttaa
     1981 agcagattct aagaagatga aaaaagcatt acttttcatt ttgtcttcaa gatctgcttt
     2041 ttaacgaatt ttatagaaag aaggcgaaac catgtttgaa cggagtcctt cagaattgac
     2101 agaacgtgaa aattacaagt tgctgattgg ttcagtcatc cctagaccag tagcagtagt
     2161 aacgactaaa tcagcaaaag atatagtgaa tatcgcaccc ttcagttact tcaatatcgt
     2221 cagttcgaat cgacccatct tatccttggc gatccagaga aaacaagatg cagtaaaaga
     2281 cacagcaaag aatattcttg agacaagaga agctgttatc catatccttg atgaggacaa
     2341 cgtagaagaa gtaaataaaa cagcagccaa tctgcctaat gaggaaagcg aactgtacct
     2401 gactgacttg actttaactg attcagtaat catttcagta ccagcattga cagaagcaaa
     2461 agttcgtttt gaaacatcct tatatgaaca tgtcgagata cggaataaaa ataaagtaac
     2521 agctgattta tttttgctag aagtacagaa atatcattta aatgaaacag tctatgatga
     2581 taagacagga aggatcgatc caagaaaatt gaatgctgta agccgtttgg caggaaatga
     2641 ttatgcagca ataggagaaa ttttcacgat tgcacgtccc gaataaaaag gagcactagt
     2701 tatgttaaaa ataagctata aaccatcgac agattcaaaa gaaatgaaaa aagaatatga
     2761 aacagtgaat gattttctgc aagggcagta cttagaagtt ccgccattgc aagaccattt
     2821 tgtcgttact actgttactt tagatggtaa ggaaatagaa atgccagatc aaacgattag
     2881 cggattgttt aattacttta acaaataaat atttattccg aaaagtgaaa aaacatataa
     2941 aaaaacacat aaaactaaaa aattatcaaa gaatcctggt gcaactgaaa gaatttgcaa
     3001 cgggattctt tcaacttatt tgttattttt ttctaaaaaa tattaaagat tgttaaattt
     3061 cactatttca tttcaaattc cattgcctaa tcattaggaa aagtctatca tgaactcgta
     3121 aatagaatat agtaataacc ttgttaggtg aggctcctat acaaacatag gctgttgccc
     3181 agaaacgtcg aaagtcgcca atgggtaaaa cagaaactgt tggattaagg ctttttccaa
     3241 tagctaagaa tgttttcttt acgttgtata gtgctaaaac tcggacgatg agatgatgct
     3301 aggtcagaat tcttgttcga aaaggtttct gaccttttct ttatattttt gtgactgact
     3361 tatttgatga gacaaactcg ctcctcacct gacaagtgga ttgctgtgtg aggaggagat
     3421 gcggtgaaaa aagatcgatt ggtggaggtc gctcggattc acttctggtt gcacaataca
     3481 accagaaaac acaacaagca aaactgatct ctatcatgag agattcatat gtcgatattc
     3541 caggttacgg aatgaataag ataaatgcag cttactctta tgggggagtt gatttattaa
     3601 atcaaacatt aaaggaaaat ttcaaatttg aagctcccta ttatgcaagt atcacatttc
     3661 aagattttat cgattgcgtt aatgaactgt ttcctggtgg agtcaagatc gatgcagaaa
     3721 aatctctaga tttagatggc gtatatataa acaaaggaga gcaagtgatg gatggtaata
     3781 ctttactcca gtatgctcga tttcgggaag acaaagaagg agacttcggg aggatcagaa
     3841 ggcagcagca agtgatcaaa gctatctctc agcagctgaa agacgtaact tcaatattca
     3901 agctacccaa agcggttggg aaattacttg gtagtatcca aacgaacctt ccagaaagtg
     3961 tgcttctgga ttgtggtatg gatttcctca aagacatatg ttagaataaa caggtataaa
     4021 tagtgaaata aaggaatgac aagcaagaga aggaggaaaa aatgaaaaga agtgacaagc
     4081 acggcaaaaa tcgaacaggc gcttatattg ccggcgcagt gatcttaata gcagctgcgg
     4141 gtggcggtta tttttactac cagcactatc aagaaaccca agcagtagaa gctggagaaa
     4201 agacagttga gcaatttgtc caagctttga acaagggaga ttataacaaa gctgcagaaa
     4261 tgacatcaaa aaaggcggca aataaaagtg cattatctga aaaagagatc ttagataaat
     4321 accagaatat atacggtgct gctgatgtca aaggacttca gatatcgaat ctaaaagtag
     4381 ataaaaaaga cgattctact tacagttttt catataaagc aaagatgaat acctcattag
     4441 gtgaattgaa agatctttct tataaaggaa cattagacag aaatgatggt caaaccacga
     4501 tcaactggca acccaacttg gtttttccag aaatggaagg aaatgacaaa gtaagcctga
     4561 ccacacaaga agcagcaaga gggaatatta tagatcgaaa tggtgaacca ttagcaacaa
     4621 caggcaaact aaaacaatta ggagtcgttc caagtaaact tggagatgga ggcgaaaaaa
     4681 cagccaatat caaagcgatt gcttcctcct tcgacttaac agaagatgct atcaatcagg
     4741 cgatttcaca aagctgggta caacccgatt actttgtccc attgaaaatc attgatggag
     4801 caacgccaga acttccagct ggagctacca ttcaagaagt agacggcaga tattatcctt
     4861 tgggtgaagc agctgctcag ctgattggtt acgtgggaga tatcacagca gaagatattg
     4921 ataagaatcc agaattaagc agtaatggaa aaatcggtcg ctcaggtttg gagatggctt
     4981 ttgataagga tcttcgtggg acaacaggtg ggaaattaag cattacagat gcagacggtg
     5041 tagagaaaaa agttctgatc gaacatgaag ttcaaaatgg caaagatatc aaattgacaa
     5101 tcgatgcgaa ggcacaaaaa atagcttttg acagtctagg aggaaaagct gcatcaactg
     5161 ttgcgacaac gccaaaaacc ggtgatcttc ttgcgcttgc tagctctcca agctatgatc
     5221 caaacaaaat gacaaacggg atctcacaag aagattacaa agcttatgaa gaaaatcctg
     5281 aacatccatt catcagccga tttgcgacag gttatgctcc tggatctacg tttaaaatga
     5341 ttacagcagc aatcggtctc gacaacggca ctatcgatcc aaatgaagtg ttgacgatca
     5401 acgggcttaa atggcaaaaa gacagttctt ggggatcttt taaaacaacg cgtgtcagta
     5461 gtgatgtatc acaagtagac ttaaaaactg ctttgatcta ttccgataat atatatgcgg
     5521 cacaagaaac gttgaaaatg ggtgagaaaa aatttcgtac aggcttagat aaattcattt
     5581 ttggtgaaga ccttgatttg ccaattagta tgaatccagc acaaatttct aatgaagata
     5641 gctttaactc agatatcttg ctagctgata ctggatatgg acagggcgaa cttctaatta
     5701 ctcctatcca gcaagcagca atgtattctg tttttaccaa caatggcaca cttgtctatc
     5761 ctaaattgat tgcagataaa gagacaaaag ataagaagaa tgtcatcagc gaaacagcag
     5821 tacaaacgat cgtgccagat ctgagagaag ttgtgcaaga tgtaaatggt acagcacatt
     5881 ctctttctgc tttagggatt ccgttggcag cgaaaactgg tacagcggaa atcaaagaaa
     5941 aacaggatgt aaaggggcaa gagaacagtt tcttgtttgc tttcaatctt gataaccaag
     6001 gttatatgat ggtcagcatg ttggaaaata aagaagatga tgattcagca accaaacgag
     6061 catcggaact attgcaatac ctcaaccaaa attatcaata aaaaattgct ttttaagtac
     6121 agtaaaagga ctgtggcgtt atcgtcacag tccttttttc aaataaacgg ttttcacgat
     6181 gtctctttca caacttcttt ttgatttact cacacttcat aaactttttt tgtaaaatat
     6241 ttgagaacaa gtggagaaat aagtgtactt aataaaatca cgataactaa aggtgaatag
     6301 tactgtggtt cgatcaattg actttgctgt ccaatttgta aaatgatcaa agccatttct
     6361 ccacgcgaaa tcataccagc accgaccatc aatgaactat gttgagaaaa accggcaatc
     6421 tgagcaccaa aataacctcc aagcaatttg gtcaaaatcg ctacaagcgt caatatagaa
     6481 ataaagagta gttgttcact aaagtcagaa aaatcaactt ctaatcctac actgacaaaa
     6541 aatactggta taaatactgc atatcctaaa gcctctacat tgtggtatac ttcttcttta
     6601 acctttgtct gactgacagc tatcccagca aaaaaagcac caatgacaga gctaagccca
     6661 acaagatccg ctacgtaaga cattcctaaa caaataacta gcgatacgat gataatagaa
     6721 gaatttgcgt acactttttc tgcaaatgaa agtaggaaag gcgcaatcca tttgactaat
     6781 aagaaaataa aaacaaaata aattagctgt tcaatcaaaa caagcggtaa agaagcattg
     6841 ctagaagctt cccccgttag aaaagatagg ctgaaactta gaactaaaac gactaaaatg
     6901 tcatctacca cagaagcacc caaaattgtt gatccttctt ttgtattcac tacgtttagt
     6961 tcttttaata cttctactga aatactgaca gaagttgccg caagaataat cccaaagaaa
     7021 atcgcttcat ttcccgaaac ttggaatgct ttgcctgtca accagccaaa aacaaccggg
     7081 aagatgatcc caagcaaagc aacgaacata ccaggtcgga aatatttttt caaaagagat
     7141 aaatcacttt ccagaccagc taaaaacatt aataagatca caccaatctc cgaaaagtca
     7201 tggactagaa tatcaggttt gacccaattc aatccagctt ggcctagcaa aacaccaaca
     7261 agtaattgtc caatcactgc tgggatacct aatctccgcg agatatgtga gccaatcgtt
     7321 gttgaaatca atatcaggca taaaatacca ataaattcca ttctcatcat tcctttgtat
     7381 aaaatatagc ccgaacttag cataacatac attattgaaa aaataggaaa atatactcac
     7441 aaaaaaacca acaatccaaa agattttttt ggattgttgg tttttatacg gtctatttta
     7501 ataactaaaa atgattaacg tatagtcatt tttcttattc tgatgaaatt gataatctcc
     7561 tggatgccca tgaaaatcaa aataaagccg aatatttgga atagaagcat caaacttttg
     7621 aatggattga acaataagac gaatccgcca atcaacaaca gaatactact ataaacactc
     7681 caacttttag cttttgtatt catgctgaag aacaactgga acaagctgtt gatgataaca
     7741 gctaatccaa gaagaatcgg caggatagaa acgattgcag gtgcaaaagc atagacgatc
     7801 agagctaaga tcaggaaaaa aagaccactg aacagaccga tactccaaga acctgttttc
     7861 cttttaaaac ggtaagcctc aaaaagattc aacaacccaa gcaaaacaag ataggcggtg
     7921 atcagataac cgataaaatg aaaaacagca gaagggttta gcacaatcgc aatcccagaa
     7981 ataatataaa cagcagctct taataaggcg tatttttgaa tattttgttt aatcgtctca
     8041 aacatataag aaaacctcct ttgacttatt ttaacgtgaa ctagtcattt taccaaatta
     8101 aatgttacaa aatgagcgaa ggagaagaat atctctggaa aaatcaactt ctgaggatct
     8161 tttccatttt tttgcctttt gctagctcat cgatcatttt atctaaataa cgaatttctt
     8221 tcatcaagtt atcctcgatg tcttctactc gtacaccaca aacaacacct gtaatcaagg
     8281 aacgtgaagg attggggaca ggggcttccg caaaaaattg ttcgaaagtc acttgttttt
     8341 gtagttgata ttctagttct gtttgggaat aacccgtcag ccaacaaatg atttcatcta
     8401 cttcctcttt tatacgatct tttctctccg cttttgctac atacatagga taaacgcttg
     8461 caaaacttgt cgtaaaaata cgattttgcg ccataatcag tcacctttct ttttttcaaa
     8521 ttatttacta cttatagtat ataagaaaaa gagaagaaaa agcgattatt caaaaaaaca
     8581 aaaaggattg tgacaaaatt cttgtcacca tccttttttc cgctctctga ctttttacaa
     8641 tcttcatagc tcaagtgcgc atcagataat cgaatgctcc taaagctgca gtagctcctg
     8701 aacccattga aacaatgatt tgtttgtatg gactatctgt acaatcacca gctgcgaaaa
     8761 cgccggggat attcgttgcg ccatttttct cggtcactat ttcatttctt ggattcattt
     8821 cgatcgttct atctagccaa tccgtattag ggagtaagcc gactagaatg aatactcctg
     8881 aaacagctag tgtatgcatc tccatcgtac ggcgatcgat ataggagaga gattctacct
     8941 gattcgtacc gctgatttct ttcgtcgctg catgtttgat aacagtgaca ttcgcaagag
     9001 agactagttt atcctgtaag atttgatctg cttttaattc tggtaagaat tctagaacat
     9061 atacatgttt tgctaaccca gccaaatcga ttgctgcttc aacaccagaa ttaccaccac
     9121 cgataacagc aatctctttt cctttaaata aagggccgtc acagtgtggg caataagcaa
     9181 ttcctttatt tttaaactct ttttcgcctg gtacattgat agaacgccag tgcgcacctg
     9241 tcgcgatgat ggcggttttt gctttaagtg atataccgtt agctaagcca atattaataa
     9301 aatgttgctc tttatttaag gaaacggcct gttgattcat cataatgtcg atagggtaac
     9361 tacgcacatg actttctact tgctgcatca attttggacc ttctgtgtaa gaggtaccaa
     9421 tcatattttc aatccctagt gtttcaacga cttgtccgcc gaaagaatca gcgacaagtc
     9481 ctgtacggat tcctttacgt gctgcataga tagctgcact actagctgct ggtccaccgc
     9541 caatcactaa tacatcataa ggttcttttt caaacaactc ttcttcaatc aaagggccag
     9601 tgattttttc aacaatttgt tcgatcgtca tgcgtccgct actaaattct tctccattta
     9661 aaaacacagt tggcactgcc atgatttttt tatcatcgat ttctttttgg aacatcccac
     9721 cttcaatcat cgtatgagta atatttggat ttaatacagc taatatattc aatgcctgta
     9781 caacgtcggg gcaattatga caagtcaagc taacataact gtcaaaatgc aatgtttgtg
     9841 ttattttttt gattttaaga ctcaaagatt cactgatttt cggtgcccgt ccactaattt
     9901 gtaacatagc cagtaaaaaa gaggaaaatt catgaccgag ggggatacca gcaaaaacga
     9961 ttccactttc tttgccaact ggattgatag aaaaactagg tgtgcgcatc gaatctcttt
    10021 tttcccaaga gattttagga gacatttctg ctatttcctt cgtaaattca gctagttttt
    10081 gactattttc actattatcc aaactaacag aaaaaacaat aggagattca atcagtgcta
    10141 aatattgttc gagattttgt ttagtttctt tgtctaacat atccaccacc taaatctttc
    10201 caacaagatc aaaacttggt ttcaatgttt cgccactttc tttccatttt gccggacaaa
    10261 cttcacctgg gtgggtacgt acatattgag cagcgcgaat cttatcaatc agtgcactag
    10321 cgtcacgacc gattccatct gcattgattt ccatagcttg aacgattccg tctggatcaa
    10381 taataaatgt tcctcgctga gctaatcctt gctcttcatc caaaacatca aaaatacgag
    10441 aaatctgatg agacggatca ccgatcatca cgtattcgat tttaccgata gcatctgatg
    10501 tatcatgcca tgctttgtga gtaaagtggg tatcggtaga gcaagaaaag acttcaacac
    10561 ctaaagattt caatgtactg tattgttctt gtaagtcttc taattcagta ggacaaacaa
    10621 aagtaaaatc agctggatag aagcaaagaa tactccagtt ccccataaga tctttttcac
    10681 tgacttctaa aaattctcct tgatgataag catgtgcatt aaattcttcg atttttttcc
    10741 caatcaatga catagtaaaa cctccaataa taattattta tttataatta ttgtaattta
    10801 gttggagttg caaagtcaat ggatattttg ttatttaata aagattactc aaaaacgaag
    10861 gttaattata aaaatagaag aggtcgcaag aaaatgctac tgctggaaat actcgggata
    10921 acgttgagta acagtttcct gttctttcag catcatgtgt aagtgcaatc ccgtttgaaa
    10981 tcgagcttct tctggttttt tatcaactaa agccttgagt aaatcataat gttgttccat
    11041 gatcgtttcc catggaagcc cctgaatggt cactcgtaac cagcggaaac gttctaaatg
    11101 ggtattatgg tcatctaacc agttccaaac ttcttttctt cctgcaattt cgaaacaagc
    11161 ttcatgaaat aaattatctg cacggaaaaa agcgcgttcg tcatttattt tgactgcttt
    11221 ttcttgttct tctaaaatcg tttctagcac tattcgtgtt tgtgtgtcca atttggaaca
    11281 acattcaatc atgatttgct cttccaactg ttctcggaca aagcgagcat tttctgcaga
    11341 aacaagatta attttggaaa catacgtacc actttgaggt atcgtataca cgagttcttg
    11401 ctgacgtagt tgaatcaatg cttcgcgaat gggagtccgg ccgatagcaa gatgttcctc
    11461 cagtgccttt tcagatattt tccttccagg ttctaagtca gcatagataa tttgtttacg
    11521 aatcgcttga taagcttgtg attgcaagct gcttttcatc tttattcctc caaaatcatt
    11581 tctatgaacg tagtataaca gaagaggtct gaaaaacaat ctggtatgtc acattaacta
    11641 gtaaatttgt ataaatagta aaattttggt tgacatagcg atttttattg cataaaattc
    11701 acttgaatac aaaaactaat atgttagttt aggaggggaa aaatgattga tttaaacaac
    11761 tggaggaact ttatccctga attatctcaa aaagaagtag aaatacctca ttttgaccgt
    11821 gcatcattaa gagaaaatgg acaaaaaaat cctgtctgga tccatttcgg tggcggaaat
    11881 ctttatcgag gatttcatgc agaaatcgct caacgtttag cggatctagg cgaattgtcc
    11941 actggtgtca ttgtttgtga aacattcgat gaagaaataa ttgaaagggc ttaccaagat
    12001 tttgatgatt tgattcttga agtggtcatg ccaagcagtg gagagttcgt caaacgtata
    12061 ttagctgcga cagctgacag tctgtattgt catccagatt gttctgaaca ctttgataag
    12121 atgattcgac actttgaaaa tcctagttta caattagtta cttttgctat tactgaaaaa
    12181 ggttatcgat tgaaagatac aaataataaa tatataggac cgattgcagt cgatataaaa
    12241 aatggtccaa agaagccatc acatacaatg agtattcttg ctgtgctact ttataaacgt
    12301 tttttatctg gagcttggcc gattgcactt gtctctacag ataatttttc acaaaacggt
    12361 agtcaattac gtacttcttt gctaacgatc gcaaaagaat ggcaaaagga aggttatgta
    12421 caaaatacat ttatccaata cttatcgaat gaagaaattg ttgcgtttcc atggacgatg
    12481 attgatcgta tcaccccgaa tccttccttg tcagttagca aaatgttggc aaaagagggc
    12541 ttttcaaata tgtcgatttt ccaaaccgca aaaggaaata atattgctcc atttgtcaat
    12601 acagaggaca tccattattt aatcatggaa gatcattttc ccaatggacg tccgaaattc
    12661 gaaaaagctg gggtattctt gactgatcgt gcaacggtag ataaagcaga tacgatgaag
    12721 gtgacgactt gccttaatcc tttgcatacg tctttagcta ttatgggttg tttactgggg
    12781 tataggagca tagctgagga gatgaaagat ccagatttat cgctgctagt ttctgaaatc
    12841 gggtacaatg aaggcctccc agttgtggag tctccaggta tcattgatcc aaagatcttt
    12901 ctggaagaag taattactgt gcgcttttct aatccaaata ttcctaatat gcctcagcga
    12961 atcgccacgg atacctctca aaaggtggcg attcgttatg gtgagacgat taaaaaatat
    13021 atggaaatag aagttaaaga cccgcagaca cttgtattca taccattgac tctcgctgct
    13081 tggcttcgat accttattgg cattgatgat caaggaaaac cattcatccc gagtccagac
    13141 ccactattga cagaccttca gcataagctt tccgatattc aactgggtac gcaagataca
    13201 tcacatattc atgagaaaat ggctcctatt ttgtcaaata aagaaatttt tggtgtagat
    13261 ctttaccaag cagagcttgg cagaaaaata gaagaaatgt tcatcaagat ggtagcagga
    13321 ccaaatgccg tacgaaaaac attacaggat tacttacaag aacatggagg aaatttatca
    13381 tggaaatgac ttttagatgg tacggatcac aggaagaaaa gattacctta gatgaaatca
    13441 gacagattcc tgcggtcaaa gggattgtag gcacattgat ggatatacca gtaggagagg
    13501 tatggcctag ggagaggatc aaagaactaa aagaagaaat tgagtctcgt gggttaacac
    13561 taaaagtaat tgaaagcgtc aatgtgcatg atgccattaa aattggatta ccttcccgtg
    13621 agcagtatat cgaaaactat aagcagacaa tccgaaactt ggcagaatat ggaatagaag
    13681 tcatctgcta taattttatg cctgtatttg actgggtgaa atctgacctt gactaccgct
    13741 tagaagacgg ctcatccacg ttagctttta tatctgatga tattccagat gatccaagag
    13801 aaatcgtcgc gcgcatcgaa cattccagca atgaatttgg attgccggga tgggaacctg
    13861 agcgcttagc tcctatcaaa tccttgcttg aagcctatgc atcagtagac gaagaaaaac
    13921 ttcgagaaaa ttttgcctat tttctccaat caatcatccc aacatgtgaa gaggtagggg
    13981 tcaaaatggc tgtacatcca gatgatccgc catactcgat ttttggatta gctcgaatcg
    14041 taaagaatcg agaagattta gattggttgt gtcgcatagt agacagccct tataacggaa
    14101 tcactctttg tacagggagt attgcagaag atccagaaaa tgatgtatat gggattttag
    14161 cagaattttg ccaaagagat cgaatcccat ttgcacatgt tcgaaatatt aaatttttag
    14221 ctaatcgaga tttctatgag gcaccacata aatccgaata cggttcatta gacatgtacc
    14281 gcatactaaa aacaatgtat gacaacggat ttaacggata catccgtcct gatcatggaa
    14341 gaatgatctg gaatgagaca ggaagaccag gatacggctt atatgatcga gcattaggtg
    14401 ctgtttatct aaatggatta tgggaagcaa tcgaaaaagg aaacaaaagt aaagataatt
    14461 agtgaaaaaa actgtaattt tattatgtaa ataagaagga tgttcaaatt cttgatacat
    14521 aaatccggag gttgttaata agcattcgga tttatgtatc ttatttggtc taggaagtaa
    14581 cttgattttc ttttttcgat tgatccatga gtgcttcttc ttttaattca aagatatatt
    14641 ctgagatcct cataatcact tttttccagt cttctggtgt gatgattcct tttaaaatat
    14701 agattttttt gatatgagaa ggcaattgtt tcaaagggca gtcgctgata acgatgttgc
    14761 agttctccat aaaatcattt tgtattttga tcgattcatt tgaaaaaatt tgttctatct
    14821 taacgatcag atctttactg atataaaaat cacgcgtata gttcacacca atcacaattc
    14881 gcggctgttt tttgtagcga tcatacaaat gtactaggtc ttcaaccatc cattgcacat
    14941 tttccttttc taaaataact ggtaacaagt gttgattttg ttctctgaaa tccatataaa
    15001 attgttcaat ggcggtttgg acttctttca atacgctatg ttcttgataa agcatgaaag
    15061 taggaagggg cgtatttggt tgattgaagt taagaaatcc attataaagt ttatttcgtg
    15121 cgaatgccaa tgagtaaaga atcttttctt tttcaggtat aaacaaatca aatacagtac
    15181 aaagggactc caccatttct cttgaataaa gaacatgagg aacgtttgct ttttttaaaa
    15241 gtttgtattg ctccaagctt gtttcttccg attcgatttt cgtgatagaa agtcgagcta
    15301 aaatattcac taagatgcgt tgttcgcgac aaattggcag attaggtcgc aaaatatcta
    15361 cagagagatc aataaaaaag tcaatatatt catactctgt gaattctttt tgtacgtctt
    15421 tcacagaagt tgcaggaaaa ttctctttac atatttttaa tagaaaatat aatttatcta
    15481 tcataccgct atttagtcgg tcaagtactt cttttttaaa aaaagtattc atcataaaag
    15541 ccactacttg cggatcttca tcataaaatt ctgtagcagt acttgaaaag atgttccaat
    15601 aaatatctaa tgctgcatat tgaatttgta cctcttttcc agaaaaagac tttctgccat
    15661 tattagaaaa ggagatagac aaaccgtact atgctaaaaa tgcattaatc tttttcattt
    15721 tcgtatacaa ataagaggca ctatagtttg tttgctgact gaaactgata atcgaatttg
    15781 tctgacagct gaacaaagaa gtcaagatct gataactgac tgattcgttg cagtatcttc
    15841 caaatagacg ataatagcag taagccggat caattccttc aaaattcaac tttaaaaaag
    15901 gtttttgctt atcaatatgc agttctgtta gattgagacg ctcaaagtct tgttctaatt
    15961 ctttgatgta tagtaaaaaa tttgattcag acatatggaa atgatctgct aaatattgtg
    16021 tagtaataaa gaaacgatgt tcagatagga aaagaaaaat atctaatttt gtattgcttt
    16081 ttttcttcaa gataaacgtt ttaatcgctt cttgagtttg catgaaagag gctccttttt
    16141 acttaaattg ttaaaacttg aaaatattac tgtttttttc ataaatttag acaaaaaaac
    16201 agtaaacata taggtagaat ctctactatc ttatttcttg catacaaaaa aatcaaggag
    16261 tgagagagtt atgagatatt caaccatctt aatatcaaca tttgcagcct taagcttatc
    16321 gcttactata aatcagacag tccacacaga ggagacagaa acagccaaat ggacagaaac
    16381 caagtgggcc aacgtcaatc gaattacctt tcaggatgaa ttttcatcct tcaattcagg
    16441 agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa gcaaaaattg actattcggg
    16501 agacttagca cctatactac aatcagctgt tacatttgag aatgttgatc ccactttgtc
    16561 gattggctat gacgagaaca ctttgactat ccaacacaag caagcgatat ttaccttcac
    16621 cgttacatta aatcaggact tgttaaaacc agcgcttttt actgtcaagg tatcagatgg
    16681 actaccgaac gatcatcatt taacgccgta tagtcagcgg cagacaattg agcaagattc
    16741 agccatcgac tcaggaggag atcttgttga agaaccaaca gataaaccag aaaatgaaaa
    16801 taagccagaa gtaccgccta cagaaaatcc agacggagaa cagaaaccgg aaatagaacc
    16861 tggagaagaa cccgatacag aaactcaacc agagccagac aataaatcaa aacctgaaat
    16921 aacccctgga gaaaaaccag atgtaactcc agaaccagat acggattctg gaaaccaaac
    16981 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aaccagaggt
    17041 agatccagaa gaaaaaccag atgtgactcc agaaccagat acggacgcaa gagaccaagg
    17101 aataccagaa aaaataaaca aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa
    17161 atcaaatcta gcaattctaa aaataaatga agagcagctg aataaaaaat cacgaatatt
    17221 tgatagcgcc caatcagctg aaaccttaaa aagtagcaag gatacaacct ttgctgtcct
    17281 gaaacgaaaa ataaacagct accaaaatcc ggagagtcac aaaacaaagt tatactgtgg
    17341 agcggaatca ttctattaag tatagcaaca atgctttctg caaaaagatt taaacaaaac
    17401 agatctctct aacatttgaa taatgtctgt gaacgcattt ttcaaataaa aagaaatatc
    17461 cagaaaatga ccccaaaata ccagattgaa atctgatatt tggggtcgtt catactttat
    17521 ctttcttcat atttatttta agggatatct gtttcttttg gaagatggtt cgggatcttt
    17581 ttcactttca atacatctaa aaagaaggta aagatcggaa tgccgacaat caagccccaa
    17641 acgccaaaga accgttcgct aacaagtaag accacaaatg tataaaaaat cggcagttct
    17701 gttttgctgg acatcaactt cggattcagc acataagact caattagatg gacaatcaaa
    17761 atcgtaatca ggatataaac aacatctcgg atgcctcctt gggaataagc tatgaaagat
    17821 agaggaatac aagaaatgat aacacctgct actgggatca gacttaaaat gaaaatcatg
    17881 atcgctaaac tcagcagctg atgaaagcca aatacagcca aggcgattgt agtgagcaac
    17941 gtatttaaaa gagcgacaac aaattgagct tctaatacca aaccaaatgt attgacaaac
    18001 ttatcggcaa aatagtaaat atcttgaaag aaccaagaaa attcactttt taaaaacaat
    18061 cttgagaata caattgtttt tttcttttca atcataaaaa agaaacttaa gatgaatgac
    18121 atagcaaacg ccactgccat ttttcctata tcctgtaaat agccaagcgc aatcgaagcg
    18181 ccattttgaa gctgttcaaa aaaatcgttt cgctcaatat aagtatggat atattgtaaa
    18241 acaggatctg catcttttgg ctgatgctcg taaaaatcaa taacggactg gatcatatca
    18301 aatgtttgat gaataagcat cgggatatac tttgtgaatg ccagatagat aaggtaaacg
    18361 atcagtgtat aaatcatgat agctaaaaca cctgtaggga ttcggacaaa tcgctgaatg
    18421 atttgaacaa gatgaatcat caaataagta aagataaatg tgagcaaaat ggttgtaatc
    18481 atacttctaa ctaagaaaag aacaaaaacg acaaataata aaacagtgag acggcgtatt
    18541 ttttcattcg ccaaaaaacg ctgataaacg gtcaaatcga caacctccaa ataacaaaaa
    18601 ataaacacgt tattcattat atgatggaat atgaaaataa gaaagagaaa gagcaaaaag
    18661 aaaaaccgct aaatgacgtt atcttatgag ttggttttcc tcataaaatc gttcatctag
    18721 cagtttcagc cctttttctg gtcttgatga aaattatcta gcatttttcg ggtcagatgt
    18781 tgcagtgtat ccaattcttc ctgttcaagt ggaagataag acttgatctt ttgtggaata
    18841 ccttctgctt gtttttgcaa cgcagcacct tgaggagtca gcgtaacatt tacgattctt
    18901 tcatcacccg tttcacgctg acgattaata taattttttt ctgctaactt ttttagtaag
    18961 ggagtcagcg taccagaatc cagacacaaa tcatttccta gctctttcac tgaagtcttt
    19021 ttcttttccc ataaaactaa catcacaaga tactgggtat aggtaagatt cagcggcttg
    19081 aggagggaag tgtagtgtct aatgatttcc ttagaggcgg catataaagg aaaacatact
    19141 tgattaacta aatatagatc ttccattttt ccacctccgc tgttttacta tagtgtatca
    19201 tagcggattt acacaaaaaa gtctattcac taactgcttt cagagtaact tctatatttc
    19261 cagctgtagc ttttgaatac ggacacactt tatgcgctgc ctctaataat tcttgcgttt
    19321 cttccagaga aagaccttcg atagatcctt caatctctac acctaagaca aagctcgttt
    19381 cactttctga ataaagggaa acttgtgcac tgacagtaga agccccagag atattttttt
    19441 gctgtttgat caattctaat gcactgttaa aacatgcact gaaaccagct gcaaacaatt
    19501 gttcagggtt ggttgcattt tcgacgcgtt taccaggagc tgcaatattc aattcgaatg
    19561 tacgatcggg attatgaact tctccagagc gtccgcctgt gttgatcatt ttcgtagaat
    19621 acattttttt cataaaatta ccacctttaa ttatatttta tacaattgaa ttttacacaa
    19681 tataattgtg gaaagcaaag aaatggattg gcataagata aataagagaa agcacatgaa
    19741 acagacgcgc tttctcgaat agcaaacata tacaaaaaaa tatcagagac aaaaactaat
    19801 ctcatcataa acattacata tatttctcaa aaagttgttt tctttcttca aaacttactt
    19861 cacgatggac ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag
    19921 tcacaccaaa gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg
    19981 ctttattatg tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat
    20041 gttcagcagt aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg
    20101 tgccatacaa ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca
    20161 gttgtatttc atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata
    20221 caaaatctat ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt
    20281 actagattct tttattatac cagttaaaga aaacgcattc aaaaaaatag cggatttact
    20341 taccaactca ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc
    20401 tgacaatgac aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt
    20461 catgatcagt cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg
    20521 cctctagcag ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca
    20581 aataatgttc actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag
    20641 tcatgacaat tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa
    20701 aaacaaacag attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat
    20761 tcaagtggaa atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt
    20821 tgtttttata taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca
    20881 acaaccgatc tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaaaca
    20941 agcttccgaa aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca
    21001 acataacgat attcaacact aaaataacta tagctcggtc aaatcctaaa acagcttcca
    21061 gtaatattcc tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat
    21121 tgatagcgac tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt
    21181 gtctcgtttc tttcatttat ccacttcctt aattcattaa taatactctt atcttattat
    21241 aacttacttt tcaccttaag ttaactttaa ttaataaaaa aattaggcta gtctaaatta
    21301 ttatgtttca ttcttctaga atatacttct acgttaaaaa ctgttaaaaa acttttactt
    21361 tagaaaattg gagaaattga tcgcttaaga taaattcagg acactaaata gtacaggaag
    21421 tagattaaaa cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat
    21481 tcggtatcaa aaaactgtta ccgataacat ttttgaaagc gttgtctttt gtttctctat
    21541 cacttatagt gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa
    21601 tttatcatcg accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc
    21661 ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt gctttgtgga gatccatata
    21721 ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca
    21781 atgttcatga ttattgggaa attgaagtga cgagcgaaac aaggcgctta cagtatgcgt
    21841 tccatgttgt tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt
    21901 atcgagaaaa tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa
    21961 tcgatcgatt cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg
    22021 aaagatttgc aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta
    22081 aagatccaga ccgagaagac ttcttcgggg gagatttgca aggtgtgagg gatcatttgg
    22141 attacttgac ggaccttggc gtcaatggca tctatttttg tcccatattt aaagccacat
    22201 ccaatcataa atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa
    22261 aacttttaaa aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg
    22321 ctgtattcaa tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag
    22381 aaaaatcgat ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga
    22441 tgactgactt accagaaact gctgaaaata tcccatatga tacttttgct tttactccat
    22501 tcatgccgaa actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa
    22561 cttattggat aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg
    22621 atcatcattt ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata
    22681 tccttggaga gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag
    22741 tgatgaatta tgcattcaca gattcgatta aagattattt tgcaaaaaag aaaatcacag
    22801 cttcacaaat ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg
    22861 aaggaacatt caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag
    22921 gtaacaaaga attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt
    22981 gtatctatta cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat
    23041 gtatgatatg ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg
    23101 tttctctaag gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag
    23161 tatatgatcg tgaagataag ctctatttca cacgggaact agagggacag atcatctatg
    23221 tttactttaa tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta
    23281 gtcaaaattg tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga
    23341 tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg
    23401 cccattataa taagggtgta acagcacgtt gaatacacag gaggaaatga agatgaaaaa
    23461 aagaagtgtg aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc
    23521 ggcttgtggc aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac
    23581 agtttccgtt gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa
    23641 agaaaacgat gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt
    23701 agcgttagat gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat
    23761 cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta
    23821 cgatgaaaca gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt
    23881 aatcgagacc cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt
    23941 taaagatttg gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa
    24001 aaatacagga tttttagcta aatggactga cttttattat tcctatggtt taattgcagg
    24061 ttacggtggc tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa
    24121 tgcaggtgct gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa
    24181 gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa
    24241 aactgcagca atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa
    24301 ttatggtgta gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg
    24361 aggaaaagga tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct
    24421 tgattatgta acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc
    24481 tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt
    24541 gattgaacaa tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg
    24601 gacaggtgca gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc
    24661 cgcagatgaa gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg
    24721 ataaccgtcc atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga
    24781 gatggaaaat tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag
    24841 tttagagggg gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc
    24901 acgctttgtt cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt
    24961 atcgtttttt tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat
    25021 gcattggtag gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt
    25081 ttgattgaag gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg
    25141 aatcttttag acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg
    25201 acgatgaagg aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt
    25261 cccgcttatc ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact
    25321 gcatttacta attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg
    25381 gctaaaaact tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc
    25441 ttttcgtgga ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt
    25501 ctcacagcgg ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt
    25561 ttcttattac catgggcggt cccagctttt attactatca tgagtttttc gaatatattc
    25621 aacgatagcg taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt
    25681 gtagatatca gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc
    25741 ttagtgcagg gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa
    25801 tcgatttcag aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc
    25861 ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag
    25921 tatacgggga atttcaataa tttctccatg atctatctgt ttaacaatgg tggtcccgga
    25981 agcgtaggcg gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg
    26041 actggaactt ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc
    26101 gttatctgtg tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa
    26161 caatgaaaaa atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt
    26221 acttttttct tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta
    26281 ctgccttcaa gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt
    26341 taggaaactt tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat
    26401 tgatcatttc tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata
    26461 cttatagtcg ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc
    26521 agatggtacc gactatggca gcattgactg ccttttatgt catggctttc ttattagaag
    26581 cgctcgataa gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata
    26641 cttggctgat gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac
    26701 ttgatggggc agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga
    26761 tgctcgccgt tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta
    26821 agttcctgct tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt
    26881 ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac
    26941 cgatttgtct attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg
    27001 gaacaaaagg ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat
    27061 tacgtacgcc aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct
    27121 tgatatttgt atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc
    27181 agatccaagc gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag
    27241 gaacccaaaa attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa
    27301 cgatagctgt gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg
    27361 atcatggaac ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat
    27421 ctggtatacg ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta
    27481 cgcccgaaga tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa
    27541 tcattttatc ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt
    27601 tattcggggc ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata
    27661 cattcaaaga gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag
    27721 cagttgtagg gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa
    27781 ctgtcttgct gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat
    27841 gacaagttta agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt
    27901 ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac
    27961 gtaatagggt agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat
    28021 tattttcacg ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac
    28081 aatacggaga tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa
    28141 tgaaagagat tacaagtaac gcttctttgg cactaggaga agcctatatg gatcatcgaa
    28201 tcgagatcga gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct
    28261 ttttacgagg aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa
    28321 aaaacaaaga agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc
    28381 tagatccaac tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac
    28441 aagcacaaat caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa
    28501 cgttgcttga tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata
    28561 atgtcaaggc aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa
    28621 taaaagaaga acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa
    28681 aaggcgaaac gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa
    28741 atcttgaagg ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga
    28801 tccatggaat cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt
    28861 tccctggcgg ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt
    28921 tgcaactgat tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga
    28981 caaagaattt tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc
    29041 gaatgtggga tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg
    29101 tgattcaata tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat
    29161 tgaggcaaaa gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag
    29221 aagtgaactt ttttgtctaa cttctaggat tgtctctaaa gcttagtttc tttttcttta
    29281 tattttcata ataaaagttt ggttctttaa tttttaaaca tgacagaaaa ttttttcttc
    29341 gttatacttg taatagaata ctattttata acaaagggga tcgaatcaat gaaaaaaact
    29401 gttatttata ctagtttatt tgcagctttg tttttagctg cttgtcaatc aaataccaat
    29461 caaggcacat ctgggaatac gtcttccagt agcgaacagc gctcttcaca ggtatcggac
    29521 tcctcaacaa aagcaagcac atcttcgtct agccagacaa aaagttctag tgaagcaagt
    29581 actcagtcag atatcaaagt gtctgcagct cgtgcgatcg aattgttcca agataaatac
    29641 ccagatactg caattactag tttggaatta gatagtgatt ggggatctta cttctacaag
    29701 atagaaggtg tcgatgatca aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa
    29761 atggagacag agaatccaga aactctagat cgagatgaac aaaatggtca aaagaaaaaa
    29821 gaagatggtc tggatgttga taaattgatc acgattgagg aagcagcaaa aatcgccgtc
    29881 gacaaagtag gaagcggtac ggcaacagat tgggatttgg ataaagattt aggtactact
    29941 tattgggaag tcaaagtaaa agacggcaat caaagtacat cagtcaagat caatagccaa
    30001 acaggcgaag tactgtcaac tgaacaagat gattaagaat ttcttcggaa aacaagagtt
    30061 gatttttctt ttagtcatga tacactagac aaaataactt gtttgaagga gcgatttact
    30121 tgagtttaga aacgaccttt gccaatcata tctttgccaa tcctttgatg aatgcatctg
    30181 gtgtccactg catgactacc caggaattag atgaactggc tcattcagaa gcaggagcat
    30241 ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca cgctattttg
    30301 atgttccttt agggagtatc aattctatgg gacttccaaa cttaggtttt tcttattatc
    30361 tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt
    30421 ccattgccgg gatgagtgtc caggaaaact tggaaatgct tgagaaaata gaaaaaagtg
    30481 ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac
    30541 tagcttacga ttttgaggcc acttatgaaa cactgaaaga agtattcagc atcttttcta
    30601 agccgctagg aatcaaattg cctccttatt ttgattttgc tcattttgat cagatggctg
    30661 atatcttgaa tcagtttcca ttgacctatg tcaatgctat caacagtgtg ggcaatggtt
    30721 tgtatatcga tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca
    30781 ttggcggaga gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt
    30841 tgaaaccaga aatccaaatc attggtacag gtgggattcg gacaggacaa gatgcatttg
    30901 aacatctgct atgtggcgca agcatgttac agattggtac agagcttcat aaagaagggc
    30961 cggaaatttt ttctagaatc ataaaagaat taacgcagat tatgtcagaa aaaggatata
    31021 cgtcaattga cgaatttaaa ggcaaattac gcaccatttc atagcaaaga gacgaaggtc
    31081 atgattgatc ttcgtctttt tttataaaaa acactcgtct gttcattcct gtgcaagcgt
    31141 actcattgta tgttagacga acttgtggga aaataacaga ggcaaaaagg aggataatat
    31201 gaatgatgca acagaattag caaaagagct taaagaaaaa gaatttagtt ttcaagaact
    31261 agttacggat atacagaaaa aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc
    31321 gcttgaacca ttcgagagta attcccacga gctattgatt ccccagtcag aaaaagggcc
    31381 attggctggt atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc
    31441 tacttcagga tctcgtttat ttgaaacaca ccgagcttct catacatcta attatgtgcg
    31501 gcaagcagaa gcgataggtc tagttccttt tggacaaacc aatgcgccag agtttggttt
    31561 caaaaatatt acagacccag tcatctatgg cccagcaaga aacccttgga accttgaatg
    31621 tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt
    31681 agctggtgct agtgatggcg gtggttctat tagaataccc gcttcgtttt gcggattgat
    31741 tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca tatgcttgga gaggatggca
    31801 aggagctgct attgatttcg gcttgacagt ttctatgaga gacacggagg ctttatttta
    31861 tgggatgaga agtatccatt ccggtgcgcc atatcaagta tcgccagttg agtggcaaac
    31921 acatccaaga aaaaaacggt tgaagatagc tttttgtgtt gattcaccga tccactcaca
    31981 gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg
    32041 gcatgatttg acagagattc cttacccact agatggtcgt aaactgatca cgtcttatta
    32101 tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg attgaaacag gcatccagcg
    32161 agctgtcaga aaagaagaac ttgaacccat ttcttggact ttgttccaat atggtcagaa
    32221 gctctctgcc gctacttaca tcgattctct gcatgtatgg gatgaagcag caattgtcat
    32281 ggaaaaccta catcaagaat acgatctatt cctttcacca acgactgcat ttaccgcccc
    32341 taaaatcagc gaagatttgc aaagtgatgc gataagagca cagatggaac acatcagtga
    32401 ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg tttgaggaga gcttgaagat
    32461 cacaccttat acacagttgg ctaatctgac tggacaacca gcaatcagct tgcctactca
    32521 tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg gctgccagag gaagagaaga
    32581 tttgctattt caaatcggct atctatttga agcaaatcat cagttccatt taccaaatcc
    32641 atggaaatag cagaaacaag gagaagaaga atgaagaaaa aaagattgtt tgctctaata
    32701 actagtgcct tttttatttt aagcgcttgc ggtacagctg ccgctaatca ggaagataac
    32761 agctggagta atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg cacactgttt
    32821 cccgcatctt attataatga tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa
    32881 gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa
    32941 atcacttctg tttcaaaaag agaatcagat tttgctgcga acgattttgg tatcacaaaa
    33001 gaaagagaaa aaaaattctc cctttcagag actttcaaat attcatttga cagtatgatc
    33061 gtacgaaaaa aagatgattc aggaatccac tcacttgaag atcttaaagg gaaaaaagca
    33121 gctggcgaac ccaatactag ctatatgcgt ttagcggaaa aacacggagc taaattagtc
    33181 acatatgaca atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat
    33241 gttattctaa atgattatta tctgcagaaa atggccgtat ctgctttacc agatatccct
    33301 gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa
    33361 ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa agaagatggg
    33421 acgacaaaga aaatatctga aaaatatttt caaactgatg tttcagtcaa accgaaagaa
    33481 acgatggctg agtcggtgag tgagtgatgt actttcccct aattgatttt tccctcatgt
    33541 ggacttcact tcctttcgta ctgcaaggct tggtatacac attagggatt ggcttcgtgt
    33601 cgtttctttt aggaaatttg gtagggttgc ttttgacagt tttaggtctt ttggattggc
    33661 tgcctctgaa tgtatttatt cgtttttatt tatcgttctt tagaggcatt cccgcactag
    33721 tcctgctgtt tctgttatat ttcgggttac cttatcaatt aagtgcctta acggcttcag
    33781 tcatctgctt tacgattacc agcagtgcat ttattggtga aatctatcga ggatcactag
    33841 caggcgtttc ttcaggtcag tgggacgctg gtttagcttc tggtttttct ttctttgggg
    33901 tattgaaata tatcgttttg ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg
    33961 tagctatgga tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg ccagaaatat
    34021 ttcaaaaagc aaagatcata ggcggtcgga cattcgatta tttttctctc tatattttag
    34081 ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa
    34141 aacgttctat ccatcagtca atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat
    34201 ttctattttt tgtttcttgt atattctgcc tagtatttta aaaccatttc atgtaaaatg
    34261 taaatcgaaa atatgttcgg aggtagaaaa catgaagacc ccgctaacca tcgaaataga
    34321 atcctcgtta taccaatatt gcatcgagca aggcggcttg gttgtagaag aagtcacgat
    34381 gcctgatgac caaggaattg tagatacgct tagctgcttg acaaaatttg atggaagccg
    34441 agaatggcga tgctatgagc tgaaagtgac aaaagcagat tttcgctcta cagccaaact
    34501 gtcatttgtt ggacattata attattttgt tttaccaagt aaactatatg aagaagtaaa
    34561 aactgaaatc cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga
    34621 gatgcctgcc tctggtacat ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt
    34681 accggaagaa gctttgacaa accgcttcat ggcttcttta ttcagggaag taagaaaagc
    34741 aaagcaaatg gagcgaggcg taaaatattt tccaaaagat caattgtaca aagaattgaa
    34801 aagacgttca gaaaatcaag atccattttc taacgaacga tattatgaac ggtttttaga
    34861 ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga
    34921 ttatgaatat ttgaggcaac aacagcagat ccgtcggttg cctacagagc cattggagtg
    34981 aaaaatatgt actatcctta tttaagagga aaacaatttg acctattagc attaaaagaa
    35041 gcactgtcgc gaggattgct ctccaataaa atccaaccag tgatcgaacc agtacgtgat
    35101 tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcc aatcgctgtg
    35161 atccaaaatc cccaagtagg acaattcaaa ttattcgatc agcatgtgca cagttggaca
    35221 gtcaaagaaa atagctctgt cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa
    35281 gtactggatt cacctccagc gtttcttgtt ttcgatggac agcactaccc aaaagataca
    35341 gaggtgtgga aacagttggc aggagttgat tcgaaatttt tgataccaga tactagtcgc
    35401 tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg actcttttca aacgagaaag
    35461 catgttgaaa gttatgcaga taaaacagat gactttttta gtgatgacta tctttttttc
    35521 cacgcagacg gatatattgg attttctgat ttcacgatcg aaggtagccg ttatttcgat
    35581 aaagggttcc ctagccgagc gcttgctatc catctaacgt atattgatgc ttacggcaat
    35641 atacgcgtga aacattttgt ctctgacagt aacgactctg ctaaagacca agcattgaag
    35701 tttcttgaag caggagataa aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg
    35761 attacttcag gaatgattga attattggct ctttaccagc aacaaaaatt ccctggactg
    35821 ggagtgttga aaaagtggtc acttatgcat catttggaac ttattagtca cctgctagat
    35881 catccaaaag attggcttaa atattattca aaagttcagg aactatatga agtgatccaa
    35941 tgattagata atgtaaatga aaaaggaata gaaaagagga aactggatgt atattcggca
    36001 aacaaggaaa gaagaagtag aagaagtggt agaaatcatt aatgccgcag cggatttcat
    36061 tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt
    36121 gaacgatatt gaaagaaaag aaagctatgt attaatagtg gaagatcaaa ttgctggaac
    36181 agctgcatta gtctcaggcg tggatcccgt ttacacaaaa atagatggta gttggaaaga
    36241 atcaactggt ccttatttat cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa
    36301 aaaactagca aaaccgttct tagaacattt gttgacgatt gcagccagca aaaatatcca
    36361 agatatccgt atcgatactt atccagctaa tgaaccgatg gaaaagacca tctattctgc
    36421 tggattcact tatcaaggga tgattgaatt tccttttgaa cacggcgaaa gaaaagcata
    36481 tcaaataatc atcaattgac tataaaatca tctgagcaaa gataaagaca ttaacactac
    36541 tgaaaaaaac aatcatatga tagccaagag gttgtaacag aagtggccag cttcaaaaaa
    36601 caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg aattttagcg tattttacga
    36661 aatagctgct ttctgaaact cccttcatcg agaggtcgtg acatagcttt tgtcacgatc
    36721 tctcggctta tcctacgagt attattcgat acatttatat caataatcct ttttttattt
    36781 tatctgcagc ttcttggaac gctccatcgt ttgacacgac taaaagtgag gggatagctt
    36841 tcaaacgttt caactgtgct aaatcttcta cataaaccgc cagtctggct gaaatctgtt
    36901 cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt ttctttagag atcgtaataa
    36961 agataggacg gactaccgtc ccgaaatact catataaatg ccaaaaacca tttgctgtca
    37021 aaacggcgta acaatttcct actgtcagtt tctttttaat ttctgcttta ctggaaccat
    37081 atttatgttg agcatattgg tcccactctg caaactcatt tcccttgatc atctgattga
    37141 atgtattgtc agaaacaaaa taataatcct cttgatcttt ttccccaggt cttggtgatc
    37201 gattcgtgta agtaatgatc ttttgttccg gctgaaaaat agcttctgcg agtgaagttt
    37261 ttccagaacc agaaggaccg ataaatacat agcaataatt cattgtcatt tctcctttga
    37321 tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc
    37381 ttttttgtat tttataccag caagccagtc acaatagcac ttaaaaagct gactaaagtc
    37441 gctgttagca aaattttcaa tccgtgtttt gcgacaagat ttcctttttc ttcatttaat
    37501 cccttcatcg caccagaaat gatcccgata gaagagaagt tggcaaatga aacgaggaag
    37561 acagatataa ttgctgttgt acgctcagta aaatggaaat taccatttgc taattctgtc
    37621 atagcaacaa attcatttgt aaccagttta gtcgccatga tactaccggc atccactgct
    37681 tctttccatg ggattccgct gatgaaggca agaggggcaa agatgtaacc taggatctct
    37741 tggaaagtga tgccgaatat cccgtggaag atagcattga tcatggcaat caaagcaaca
    37801 aaaccgataa gcatagcagc aaccgtgatc gccacatgga aaccatcaag aatgtattct
    37861 cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc atcttctttt
    37921 tcttctaata catagggatt tacaattgaa gccagaatat aaccgccaaa taagttcaaa
    37981 acaagggcag tgatcacata tttagaatcg atcagtgcca tataagatcc gacgatcgac
    38041 atagaaactg tagacatagc cgatacactc atggtgaaca aacgtttctc agataggaag
    38101 ggtaattcct tttttatgga aataaaaact tccgattgtc ctaaaatagc agaagctact
    38161 ccattataag attccaattt gcccatcccg ttgattttac ttaatcctag accaacagct
    38221 ttcataaata atggaagaat cttgatatag cgtagaatac cgataatagc agaaataaat
    38281 acaattggca tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc tacaccggca
    38341 aaaacaaagt caacaccttg cccagcaaaa gcaagaagat gatcaaacat ttttgccaat
    38401 acagaaacaa ccgctgtacc aaaagttgta cgtaataata cgaaaccaag aacaagctgt
    38461 aaaacgaaca tcacaattag tggtttcaat ttgattgctt ttttgttact gctgatcaaa
    38521 aaagcaagcc ctaatgtgac aaataaacca ataataccta ataaataact caaataatca
    38581 ctcctattcc aaattccgtt ttattttacc tgttttgcaa ataaaatact aatctttagt
    38641 gaaattttca ttactttttt agagtgaaac gcattcattt tccattagaa aataaagcga
    38701 acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag tctataaaaa aacgaaacta
    38761 tcgattaagc taatagtttc gttttcaaca tttataagta aactttttct attggtaata
    38821 tagattttcg gaagggtaga caggtgcgca ggtaatatcg attcctagtt ttttcaatgt
    38881 ttgttcatca tttttattga ccatcacagt agagtgggct tgtgctccct caagttcagc
    38941 tagtttatca taagcaactt gagcgattgg attcgttact gcactgattg ataaagcgat
    39001 caagacttca ttgatgctta gtgcatccaa agaggaatga agtgtctttg atttcaatcc
    39061 ttgtattgtt tcaagaacta atggtgacaa taaaggaatg tcatctgcga tatgggctaa
    39121 atgttttact gcatttaaga taactgccgc tgacgcatcc atcaatgaac ttgtacgccc
    39181 ggtaatgatg acttcatctg gtaattcgat tgcaatcaca gcagaaggat cttttgtagg
    39241 actttgcgct tgaatcttat ctgcatagtc atgggcaggg actaccacgt ttcggtcttc
    39301 aggacgtaaa ccgacttctt ccataatcag tttgattcga ttaactgctt cttcgtcgat
    39361 caatcctttt ttgaagtcgc attctgtttg gaaataacga cggatgattt cttgtttaga
    39421 ggcttcttgg acaacggtat catccacgat cccaaatcct acacgattca cacccatgtc
    39481 agtaggagat tgataaacag attctttacc agtaattttt tcaataatac gtttgattac
    39541 aggaaatgtc tcaacatccc gattatagtt cacagctact ttattgtagg catcaaagtg
    39601 gaaggaatcg atcatattga catcttttag atcgactgtg gctgcttcat aagcaatgtt
    39661 cagcggatgt ttcaacggaa cattccatac tgggaaagtt tcgaacttgg agtaaccagc
    39721 tgcatgtcct ttttggcttt catgatacag ttggttcaag caagtagcga gctttccact
    39781 tcctgggcca ggagctgtca ctacaacgat tggtttagtt gtttcaatat actcattttt
    39841 tccaaaacca tcttctgaga cgattttttc tacgttgact ggatagtctt cgatttctga
    39901 gtgtttatag accttgatgt ttcgtctttc tagtttattg ataaagactt tcgtggcagg
    39961 ctgtccacta tagcgagtga taacgacact gttgattaat aagccataac cacgtaattc
    40021 atcgattaac cgcaagatat ccatatcata agtaatccca taatcaccac ggatcttatt
    40081 acgttcaata tctccagcat agacgcaaat cagtatttca gcttgatctt tcaatttttg
    40141 cagcaatttg attttcgcat cttcttcaaa tcctggaagt acacgtttcg catgcttgtc
    40201 gtcaactaac ttcccaccaa attctaaata taatttgtca tagtggttca ctcgttccaa
    40261 aatatacgct gactgctctt caatatattt ttctgaatca aaaccaattt ttttcactag
    40321 gttccctcct taagtactaa agcatttttt catttttttg aggattcgtc aactggaaga
    40381 gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag atagttattc
    40441 attggtatga tagtttttga taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa
    40501 acgcatataa acactatatc cgttcgattt ttaccggtta tgaaagaatg cttgaagatc
    40561 ccgaattgga acaattgacg tttacttatc taaacgaaga aacgcagcta actcgtgatg
    40621 agcatcagcg cattcatttc acaacaagag atctgcctag taaataaaaa ataagataaa
    40681 ggtcatcgga ttcgattctg tcctatcaga tggaaagact agttgatcga aaaaaaggct
    40741 gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc cgctcttttt tataacaatt
    40801 ttttacagaa aatggtacta ttgaagtatt aataagtgaa aaacagatga gtaagaggag
    40861 gcaagtagta tgaaagagcc agatacaaat gcaaattatg agccagacga actagaggag
    40921 acgaacgcgg atcaggatac aggacaagaa tcgtcttctt tttacgatat caaagaaaaa
    40981 gcaagcatga atctttccca attattagaa gacttacagg cttttgaaaa agctgtccgc
    41041 gaagaagata tccctgagat ctaccgttta tacaatggcc gattgaatga agaattaaaa
    41101 agaagctcaa atgaaaatca cgagatcgat caattgctga tgcgtaagat ccatgatcgt
    41161 tttttacagg aatttccttt tatggaacaa gtagaaaaca tctcgccaac gatgagctat
    41221 tacaaaatcg gcacttatta ccatgatcgg cccacggtcg gtattgatgc aagtctgcca
    41281 gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga aatattccca accggagtca
    41341 acggactatg acaaaaaaat caatgagcta gacgcaaagg tgatcactgc tcagacagaa
    41401 attgaacgtt tagatgaaca aataaaagaa atcaatcgac aaatgactga tttgaacgac
    41461 aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag
    41521 aagcaagtac tatctaatga aaaattaggc tggcttcctt atattgagac gcctgaaaca
    41581 atccagcagc aaaaagaagc attgaagcaa gaagcaagag cagaccagct tcgtgcagcc
    41641 atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga gggattcggt
    41701 caagcaatcc atgaattctt gatgaattat cttggcaatg agaatcctaa taatcaatct
    41761 aatgaaggag gttccgagta tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga
    41821 acaaacagaa gatcatgaag aaacgaacac ttcattggaa gaacaaacaa gtaatcctct
    41881 ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa atacatgaaa aagaaaaagt
    41941 aactccgacg aatacgaata ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc
    42001 cgggtattta gaaggaaaag agcaagatca tcaagatatc gtcagtttga ttgaaacagc
    42061 ggaagcagaa ttagcggatt tacgtatgag aaaagatttg atcgaatcag attttccaca
    42121 attattagaa tcatatgatc gttttatatt gaatgaagaa ccaatctcca cgatcggaaa
    42181 gacaagcctg atcgaatact tcacgtatga attactgaaa ccgttgaatg atattggtct
    42241 ggaacggtca aattcagctt atgacacatg gaaaacaaga catttttcga tcaattatac
    42301 gttgaatgga caaaacgaat tagtcttttc ctttgtatta ccgaccatcg atcaacgata
    42361 ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag acaatggaaa tcaatgtcca
    42421 agatgaccgg gtgctttctt tgatccgaca ctggtcagtg gatcgagtat tctctgcagg
    42481 gcagatcacg atattcaatc acaaattgaa tcaattatta gcccatgcaa gaacacttgg
    42541 ttttaccgtt aatcagacat tactagacaa tacgaagcca cttcgtttga atcttcagag
    42601 cgaatttgaa ttgacgaatg aagtattgga tgatattttt atcgtggcaa tgaaaaatcc
    42661 atcctacgat atggaaaaga tcaacgaaac tacttatcaa gtacgattag ataaaggaca
    42721 gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct catctggaga
    42781 ctacccacga tctgtcattg atttctttat caattacgaa ttcttggtcc cattaatggt
    42841 gagaaagtcc tgattggata agttggatta ccagatgtca ttcagataat tctgttccaa
    42901 aggacttgcg gcagttatcc agaataattg tcacaagtct tttctgtatg tactaaatgg
    42961 acgtgaataa cttgctgatg aaggagggat ttcattagga atgtccacaa ataaaatagc
    43021 acagcttagc ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc aatttattcc
    43081 gaatttccag ccaatgactg ctattttttt atttatagta ctttacttaa gcttaaaaga
    43141 tgcgttaatt gtcatgagtc tttctattgc gatcagcagt ttttattttg gtgttggacc
    43201 atgggtcatc ggccaatgga tcagctatac tgttgttctg agtttgtttt caatcgtctg
    43261 cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga gtctgttttt ttctggcggg
    43321 catggtttac ggaataggta tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc
    43381 ttggatctat taccttcaag gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt
    43441 gttctttctt gtttttctgc cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa
    43501 tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt
    43561 gcagtacaac ctatacacaa tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa
    43621 aagcaacaat cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa gtggtcttca
    43681 aaaaaggcga tgatttactg agtattttga aacgaaattt tgaagtcaaa gaagataacg
    43741 gcttcatcac ttcgcttgaa gggcactcac aggatgagaa aaatcaactc tattggatgt
    43801 ttactgtgga cgggaaaagt gctactactg gtgccaaaga gatcaaattg aatgatggac
    43861 aatcggtcat tttcaatttg tctaaactat aagtttcata aaaaactgtt aaagggcgaa
    43921 tattgccata attcagtgag ttcagttgat gaaagaagga aaataacatg gaaaatttta
    43981 aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc taatattcta gttgctatca
    44041 gcaaatttgt cggctatgca atcagtggca gtgccgccat gctaaacgaa agcatccaca
    44101 gtatcgttga ttgcagcaat cagattttgc tgttattcgg tgataaacaa gccaataaag
    44161 gacagagcga gttgcatcaa tttggtgaag gacgagcaaa atactttttc agtacaatcg
    44221 tcgcaatgat gcttttcttc ggcggtgggg cacttggggt gatggaagct tttgaaaagt
    44281 tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt tatagcaata ctgttatttg
    44341 gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata
    44401 ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa atactgatta
    44461 tttttacgga agacttgtgt gctgtaatcg gtttggtctt ggctttagca ggaacaatat
    44521 tgacttatgc aaccgggatt gctgcatttg atgcgatcag cggattgctg atcggtttct
    44581 tgctgatggg agccgccatt ttcctagcaa aagaatttta tagtctgatc atcggtgaaa
    44641 gcgtgacagc aagcgattta tcaaaaatca agttagcttt tgaacgtcct gagatcagtc
    44701 ggctgatcga tataaaaaca gttcatttaa gtccaacgga gattttagtt gcagcaaaag
    44761 cagatatcgt tggtaacaaa gagaatgaag cttactcggt catcaatgat attgaaaaaa
    44821 atatgcgtca gtctcttcct gacaagaaga tgtatatata cattgaaaca gataaatatg
    44881 acccaaatta ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa
    44941 tataatcttg tttttaaata aaacggctac aatttagcga atttatgtta caatgagtcc
    45001 gctgtttatt tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa agtgcgccat
    45061 ggcttcaagc tttgcatgca gtgatgccac tttgtataag ctatatacca gtaggattag
    45121 cttgcggagt attgttgcaa aaagttggat ttgatcctct cttatcagga ctgttatcga
    45181 ttctggtctt ttccggtggc gcgcagtttt tagttgcatc tatgcttacg acgcaagcat
    45241 catttgcaac gacattattg atggttttct ttttagaatt acgctataca ttattaggag
    45301 caagtcttgc cggttttatg aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat
    45361 cactgaatga tgaaaactat gcggtgaact acttaaagtt ttcaactgac ccttcctgga
    45421 ataaaagaaa ggctttatat gtcaattggt tctcaatgac tgcatgggcc ggtagcaata
    45481 tgatcggtaa ttttttcggc tcggtgattc gagtagatgc tgaccttgtc cattttgctt
    45541 tgacggccat gttcattttc atgtttatca tgcagatgaa aagcgcacta ctgatcttta
    45601 caggtttatt ctcaggcgta ttaggggtcg tattcatggt attattccaa aatactttcg
    45661 gattgattgc agcaacagtc attgcttcat ttgccggatt tatgctggag aaggcattca
    45721 aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca
    45781 acttcccgga acaggaggcc aatcaccatg actaaactat atttgctact tttagttgct
    45841 tgtctgttca tcgtggcata tatcccgcgc ttgttcccga tgctttattt tactcatcga
    45901 aaagtgccat cctggttcag tgactggatg aaatatgtac cggtggcatt atttgcggcg
    45961 ttagctttta aagatgtttt cattactcat gaacatctgg atattgcttg gaatatcaaa
    46021 atagctgcaa tgattctagt agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca
    46081 gttttgacag gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt tttattttat
    46141 taaaaacaga gattgcgaca taagttgttc aaactgatct atccgaactg catttgcaac
    46201 agtcaattct ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt ggcttatgat
    46261 gcaagctctt ttttatttta ggaattagag agggatttgt ccttctatga ttttttgcta
    46321 taatagggaa gatgtttatt ttatgaaaga atagttgagg gaatttaaat gaataaaaaa
    46381 ttgatgcaag gaacattttg gctaaccttt gctaatcttc tatgtaaagt tttaggggta
    46441 gtctatttga ttccctggct aagtatgatg gggaacaatc aagatggtat gcttgctacg
    46501 acattgtata atgttggcta tcttccttat ggattgttcc tgatgcttgg tacagtcgga
    46561 tttccaaatg cgattgcaaa aaaagtagct gtagccacaa aagaaggaga taacgctgct
    46621 tgtcggctga tttttagaag tacgatcaat atcatgtttg tcatcggtat cgcctctgct
    46681 gcactgatgt atttatttgc tcctttacta gccggaatca gtccgattgc taacgttaat
    46741 aatgggatac tagctatcag aagtttatgc ccatctttga ttgctatccc aattcttagt
    46801 gcaatgagag gatatttcca aggaaaaaat gatctgcggc cttatggaac ttctttgatt
    46861 atcgaacagg ctgtgcgagt gatcgttatc ttggctggta cttattactt gcgcgtattg
    46921 actgacggaa ccatattaga agctgtattg atcagtacag tcgcttcttt cttcggtggg
    46981 ttagctgcga ttatccatat gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa
    47041 gattttttga tttccagccg ttatttcgaa agagaaaacc gatctgcctc tgtctctatc
    47101 attcgggaaa cactaccgtt tatttttgtc ggctcagtca ttactatcgt tcaaatgatc
    47161 gaccaagtaa cgatgaaacc actgcttcac tttttccgtc cagagatcgc agatcagcaa
    47221 ctggaattct tattcagccg tgcttctgtc aatccaaata aattgacatt gatcctgatc
    47281 tccatggtag gaacagtagc tataagcagc ttgccaatat taagcacgat gaagaaaaaa
    47341 gatcggatac aaatcgaaaa aacagtagga gatagttttt cgattgcgtt attgattctt
    47401 cttccttcat tgactggaat gtctttatta gctggaccac tatacacatt gttttttggc
    47461 tatgatcctg aaagtgtcgg atatttccaa atggctttgc tagcttcttt gtttttctct
    47521 ttattcacga tcctatctac gatgcttcaa tctttgaatc accatcgttt tgcgatccga
    47581 ttaactgtag aagcaattat cctgaaagtc atcttccaag taatcggatt aggattagtt
    47641 ggcggatacg ggatgagctt atcaaatggc ttagcattcg gtattgtctt tgttcgcggc
    47701 tatcacttta tgtgcaaaga ataccgaata gcacctttag caaagatcag caattttttc
    47761 ttaaaaactt tccgctccac cctgattatg cttgcagtat gttttgccgt tttctttttg
    47821 ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa tgatttattg tgtggctgta
    47881 ggaagtattg gcggcttagt atttttattt tctcaattcg gaaaaaatgg gtttcgaatg
    47941 ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg ttaactaatc gttttttgct
    48001 gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg tttttctatt ttccaatttt
    48061 agtttcatat ctaatcttac ttatgatata gttataaggt ctggaaaaat tctttgaaac
    48121 gaggaaatta aacacatgaa acttgcagta gttacagata gttcggcata tctgccggaa
    48181 cgtatcaaga accatcccga cttattcgtt attccaatcc ctttgattat ggatgggaaa
    48241 atttataacg aaggaatcga tatcgaagcg gatgaatatt atggactttt aaaaaacagt
    48301 aaagattttc ctactacttc acaacctgtc gtaggggaag ttttgggttt atatgaggaa
    48361 ttgaaagaga aagggtatga cacgatttta agtatccatc tttcttcagg gatttctggc
    48421 tttgtcaaca cattatttgc gatgaaagat gacatcagcg gagtaacggt cattccttat
    48481 gactctaaga tcactagtat gccgatggga cacatggtag aagctgcctt agacttaaat
    48541 gatgaaggtc agtcccttga tgaaatactt gcacatatcg atcgaatccg agataatacc
    48601 tatgcttatc tgattgtaga tgatttgaac aatttagtac gaggaggacg tttgacaaat
    48661 ggtgcagcgc tgattggcag cttattaaaa atcaaaccga tcctgacatt ttctgaaggg
    48721 aaaattgttt tattcgaaaa aatccgttca agtaaaaaag catttgctcg agctgaagaa
    48781 attattggta gaagaaataa cgaaatacaa ccgccagtca aactttacgt gatacatgca
    48841 aataatttag aagtagcgat tgcggagaaa gagaaattgc agaaaaaata tccaaatgca
    48901 gaaatcgaaa ttggtcattt cggaccagtc attggtacac atttaggtga aaaagccatc
    48961 ggcattgcca tttcagcgca ataattcctt ttattcaaaa aacataccaa aaatat
//
LOCUS       DACDYM010000012        47631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDYM010000012 DACDYM010000000
VERSION     DACDYM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.012"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IWM59_000720"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IWM59_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9978773.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IWM59_000721"
     CDS             1995..4661
                     /locus_tag="IWM59_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9978774.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNFPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IWM59_000722"
     CDS             5025..5777
                     /locus_tag="IWM59_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9978775.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IWM59_000723"
     CDS             complement(5799..6953)
                     /locus_tag="IWM59_000723"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9978776.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IWM59_000724"
     CDS             7138..9579
                     /locus_tag="IWM59_000724"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9978777.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IWM59_000725"
     CDS             complement(9909..10613)
                     /locus_tag="IWM59_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978778.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IWM59_000726"
     CDS             complement(10800..11045)
                     /locus_tag="IWM59_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978779.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IWM59_000727"
     CDS             complement(11062..11913)
                     /locus_tag="IWM59_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9978780.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IWM59_000728"
     CDS             12079..13083
                     /locus_tag="IWM59_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9978781.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13324..13680)
                     /locus_tag="IWM59_000729"
     CDS             complement(13324..13680)
                     /locus_tag="IWM59_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9978782.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            14048..15235
                     /gene="tuf"
                     /locus_tag="IWM59_000730"
     CDS             14048..15235
                     /gene="tuf"
                     /locus_tag="IWM59_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9978783.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IWM59_000731"
     CDS             complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IWM59_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9978784.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(18040..18759)
                     /locus_tag="IWM59_000732"
     CDS             complement(18040..18759)
                     /locus_tag="IWM59_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9978785.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            18968..19600
                     /locus_tag="IWM59_000733"
     CDS             18968..19600
                     /locus_tag="IWM59_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9978786.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            19659..20519
                     /locus_tag="IWM59_000734"
     CDS             19659..20519
                     /locus_tag="IWM59_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9978787.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20626..23019
                     /locus_tag="IWM59_000735"
     CDS             20626..23019
                     /locus_tag="IWM59_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9978788.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23201..25042
                     /locus_tag="IWM59_000736"
     CDS             23201..25042
                     /locus_tag="IWM59_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9978789.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            25066..26175
                     /gene="rpoD"
                     /locus_tag="IWM59_000737"
     CDS             25066..26175
                     /gene="rpoD"
                     /locus_tag="IWM59_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9978790.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            26395..28494
                     /locus_tag="IWM59_000738"
     CDS             26395..28494
                     /locus_tag="IWM59_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9978791.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            28690..29553
                     /locus_tag="IWM59_000739"
     CDS             28690..29553
                     /locus_tag="IWM59_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9978792.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            29658..30131
                     /locus_tag="IWM59_000740"
     CDS             29658..30131
                     /locus_tag="IWM59_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9978793.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            30227..31114
                     /gene="xerD"
                     /locus_tag="IWM59_000741"
     CDS             30227..31114
                     /gene="xerD"
                     /locus_tag="IWM59_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9978794.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            31133..31504
                     /locus_tag="IWM59_000742"
     CDS             31133..31504
                     /locus_tag="IWM59_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9978795.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            31474..32271
                     /locus_tag="IWM59_000743"
     CDS             31474..32271
                     /locus_tag="IWM59_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9978796.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            32255..32824
                     /gene="scpB"
                     /locus_tag="IWM59_000744"
     CDS             32255..32824
                     /gene="scpB"
                     /locus_tag="IWM59_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9978797.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            32830..33546
                     /locus_tag="IWM59_000745"
     CDS             32830..33546
                     /locus_tag="IWM59_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9978798.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      33628..33753
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            33876..34487
                     /locus_tag="IWM59_000746"
     CDS             33876..34487
                     /locus_tag="IWM59_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9978799.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            34865..36388
                     /locus_tag="IWM59_000747"
     CDS             34865..36388
                     /locus_tag="IWM59_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9978800.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            36625..37965
                     /locus_tag="IWM59_000748"
     CDS             36625..37965
                     /locus_tag="IWM59_000748"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9978801.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            38118..38855
                     /locus_tag="IWM59_000749"
     CDS             38118..38855
                     /locus_tag="IWM59_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9978802.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(39020..39241)
                     /locus_tag="IWM59_000750"
     CDS             complement(39020..39241)
                     /locus_tag="IWM59_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9978803.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            39272..40303
                     /locus_tag="IWM59_000751"
     CDS             39272..40303
                     /locus_tag="IWM59_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978804.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            40290..41693
                     /locus_tag="IWM59_000752"
     CDS             40290..41693
                     /locus_tag="IWM59_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9978805.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            41759..42394
                     /locus_tag="IWM59_000753"
     CDS             41759..42394
                     /locus_tag="IWM59_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9978806.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            42443..43123
                     /locus_tag="IWM59_000754"
     CDS             42443..43123
                     /locus_tag="IWM59_000754"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9978807.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTREVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            43258..44487
                     /gene="rpsA"
                     /locus_tag="IWM59_000755"
     CDS             43258..44487
                     /gene="rpsA"
                     /locus_tag="IWM59_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9978808.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            44669..45979
                     /locus_tag="IWM59_000756"
     CDS             44669..45979
                     /locus_tag="IWM59_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9978809.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            46222..46497
                     /locus_tag="IWM59_000757"
     CDS             46222..46497
                     /locus_tag="IWM59_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9978810.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            46732..>47631
                     /locus_tag="IWM59_000758"
     CDS             46732..>47631
                     /locus_tag="IWM59_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978811.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEK"
BASE COUNT        15823 a         8288 c        10037 g        13483 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact tcccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac
    13201 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
    13261 ttcttggagt taaacgcttc tgtcccagcc tctttttatc taataaaatt ttaagaccat
    13321 ttactaatta tcgatttttt ctgccgcatt tttaggcact tcagaagcct ttatttcttc
    13381 ccagcttaat acacctttac gaggattgac tttgagttta agatacgcat tcatctttaa
    13441 tggatgttct ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt tattataagc
    13501 cgcttgcttg taggtataag tcgtgaattt ctctccgtta tctgcttggg tagctgacat
    13561 ttcgccattc gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat aatgataagc
    13621 tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt acgccaatta attttttcat
    13681 gtacttcacc tctttcctta ttattaacga aatagaagat ttcgtctatt gaaagttgtt
    13741 ggcaacagct gaattttaaa cttttcctca ttttgttctt attgcttcaa aagaaagtta
    13801 aggactatct tgaagttttt ctcaaaaaac acattttttt atttataaaa ggtacttttt
    13861 tcgcgtataa gacagacgag tgctcttagt tgactttgac caatattgac gtataataag
    13921 cttagttcga atcttacaaa aagtaagtcg gacgtattct ttttgattta gtcaaaaaaa
    13981 gactataata aaaaggtgag agctgaaatt tttcagtgat ctatattatt taggaggttt
    14041 gtaaattatg gcaaaagaac attatgacag aagtaaacca cacgttaaca ttggtacgat
    14101 tggacacgtt gaccatggga aaactacttt aactgctgcg atcactacag tattaggcaa
    14161 aaaaggccta gcaaatcctc aagactatgc tagtattgat gcagctccag aagaacgtga
    14221 acgtgggatt acgatcaata ccgcacacgt agaatatgaa acagaaaaac gccactacgc
    14281 gcatattgat gcaccaggac acgcggacta tgtgaaaaac atgattacag gggcagcaca
    14341 aatggatggt gcaatcttag ttgtttctgc aactgacggt ccgatgcctc aaacacgtga
    14401 acacatttta ttgtcacgcc aagttggtgt aaaatacctg attgttttct tgaacaaagt
    14461 tgatttagtc gatgatgaag aattgatcga tttggtagaa atggaagttc gcgagttatt
    14521 gagcgaatat ggtttcccag gcgatgacac tcctgtgatc aaaggttccg cattaaaagc
    14581 attgcaaggc gatccagatg ctgaagctgc tattatggaa ttgatggata cagtagatga
    14641 atatatccca acaccagaac gtgatacaga taaaccatta ctattgccag tggaagacgt
    14701 cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg gtgctgttcg
    14761 tgtcggtgat gaggtagaga tcgtagggat caaacctgaa acacaaaaag cagttgtaac
    14821 aggtgtagaa atgttccgta aaacgttaga ttacggggaa gctggggata acgtaggcgt
    14881 gttgttacgg gggatccaac gtgacgatat cgaacgtgga caagtacttg ctaaaccagg
    14941 ttccattact ccacatacaa aattcaaagc agaagtgtac gtgttgacaa aagaagaagg
    15001 tggacgtcat actccattct tcaacaacta tcgtccacag ttctacttcc gcacaactga
    15061 tgttacagga acaatcacat tgccagaaga tacagaaatg gtcatgcctg gtgacaacgt
    15121 gacgattgat gttgacttga tccatccaat cgctgttgaa aacggaacga cattctcaat
    15181 tcgtgaaggc ggacggacag taggttcagg tatcgttaca gagatcgaag cataagaaac
    15241 gcgactcgaa tattttggta atgaaaaatg tttaaaataa aagagcggtc caaacaggat
    15301 ttctgtttgg accgctcttt gttcttcaat tgtataagaa gagattgtag aagcatgaac
    15361 tttcgtttta cttcagtctc ttttattcat catgagagtg ttcatctatc ggtatgcagt
    15421 ttttaatact ttcttcgtat ccattttttc aaaccactta ttctaccttg tgtctgctca
    15481 actggcgcat tatcaataat cagcaaagca atcataacat atgcaatcca actatctcta
    15541 gaatatagag tataacgtga gacccagttt gtagcgtact tgcttttata ttctcttaat
    15601 ccatggaaag aataaaaatg agaaccaaac tcgtaaacca agtatgcaat cctttcctgt
    15661 aaaaaactct ttctggaagt tccgacattt gaaagagggg ccatccctaa attgaaccat
    15721 tggatatgtt cttctttcat ataggcaaac aaatgcaaga acaagaaatc catactgcca
    15781 gaaggtgctt ttttagaatc atatcgcatc agatcaatcg ttcctacttt attgttcgtg
    15841 taagtaggca tgatcgttgc aaaagcgaca atttcttctt gggtattttt gacaactgca
    15901 ataggcgctc tttgtagata atcttcagag aaaaagccta aagaaaaccc tttttccttt
    15961 cgtgaaccca accaattatc agagatattt ttgaaagtac tcatttgctc agtagaaaat
    16021 ggtggttgta agacatcaaa tgtaaatcct tctcgttcga tccgattcaa gacagcacga
    16081 gtgcccttca tttttttccc tgaagttgta aaactattca ggtctacata agcttcttcc
    16141 cccatcttaa tgaaatcgta gccgaattca tgcaaaatca taacaatctc ttcgcttgtt
    16201 tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt cgataaaagc ttcgattgct
    16261 tctggaaaat cttctttctt accagaagga tcacccatta caatacattt attattgaag
    16321 caagcgaatt ggagaagaac agttggttca ccgtcttttt cataggcaaa catccgctta
    16381 tcttttaaga agatcaattg actatcggag tttcctccat aagttgtaag gatcttcagg
    16441 gccttttctt cattaaaggc ttcgccaatc tgtttctttt ctccttgaag gaaatgaaca
    16501 aatagaacga tcatgaaact aacagcgatg atggccaaca atcccgaaaa ccaaatcttt
    16561 tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa atctggcaga
    16621 ttgtataccc caataacgat gtaaagcaag gacagtgcac caataatgat tccatcgatc
    16681 gtacgccact cccatgaata gatcaattgt tcccgaaata attcgttttt agaagcaaga
    16741 atgatcagaa gcagaataga caagaaaacc accggtgtga aactaaaatc acttaataaa
    16801 acataaagta aagctagaac aatcaagaag attgtaggca aatatgctcg tttcacacgt
    16861 gctgcgatcc ctctgcccat aatgattaaa agaaatccca atagtatact aggaaattgg
    16921 ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc tgtaaaagct
    16981 tgcgggatag tggcagaaag tactagcatg attccagaaa agtataataa gacaaccacg
    17041 attttatgcg caatttctgt tgctaattgt ttagggatac cagaataacg ctgatcaaaa
    17101 gtggaaccga tatttttgaa gaaaaagacg attccaatca aaaacggaat gatgtagtag
    17161 aacaggcgat agagcaagat ccacacaacg actatttctc ttggcacacc aatagcggac
    17221 aacccgataa tcatcattac atcgaaactc ccaatttctc ctggaatcat agacacgatt
    17281 cctatcactg aagcagcaat gaataatgga attgtttgcc acagatcaat ccggatttcc
    17341 attaaataac caacagaaag gaaactaaac aggacaccgg tccattccaa caatgaaact
    17401 agcaatagct gtccttgagt tttaagtgag gtaccaccaa ggaatccttt ctttttgaaa
    17461 gtagtaaata aaaagacgac aggaaaatat agaccgccgc cgatcaacca tatccaatat
    17521 tgcttcaaaa aaggagcaac tggcgtgaag actacaagta aaaaagaaag cagactgtaa
    17581 atagaaagtc cagccatcaa aaacaagaaa actttcgata atgcttggat cacgtttctg
    17641 ctctctttct tttgtccata tagttcagag cgaagcccca cactaacaaa cccgccgaaa
    17701 ccggcaatat tgttgatcgt attgatcagc cagcttgtct caaacaggta acgaggattc
    17761 tgcttttgtt caagcatccg attcaaaata atatcataac caaccatagg taacacagag
    17821 ataagaccaa tcaatagcat tagtccgctt ttccatacag gtatagtggc aaacgtttct
    17881 cctaattgct ggatagatag agttttaccg atggacatca attcaccgac aataatgaca
    17941 atgacagaaa tcaaaaagat tgttttgaac agtcctaggt ggtttttcag ccattgtatg
    18001 gtatttttta acaaataaat caccttctat tcctaatagt tatgtttcag aaagaaattg
    18061 aagaacttct cgattgaagc gttcaggatc ttttctagct aattcatgtc cttgttcttt
    18121 cacaagcaca aaggaagctt ttggtatagt tttagctata tacaaagaat gttttagctt
    18181 gattacatct ttttttccca cgatgatcaa agtgggggag ttgatttttt ttaaatcatt
    18241 ttctgtaagc ccgatgtcat gcagcaaaag tttgataact aaaagatttt tgcgaagact
    18301 tgggcgaaat aaagagagta accataccca tgcataatga atattactga tgactctggc
    18361 agaaaaacga acgcctttaa caagcgtgtt tccagaattt aaaatcaatc gatgaacttt
    18421 tttagggaag ctgctggcaa ataccagtgc tagatttgct ccatcactaa atccaagaaa
    18481 atctgcctga tcgatttttt ccagcaacat gatcgtattc agatcctcgg ccattaactg
    18541 aaaattcaac atagatgctt cgtttgtcga acgaccatgt cctcggctat ctactagata
    18601 cacggtatag tagcgttcga gtacagggac ctgttctgaa aaaaagcggc cgctgccgtc
    18661 attgccatgc agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt aaatttcgct
    18721 cccatctggc atttttgcat atttcttttc ttttttcaca taatcaccta ctgtgtaaag
    18781 tataacgatt atatcagtca ttgggcaatt gtttttactg aaaaacaacg atcattttcg
    18841 taagatgtca aaaaatacag gaaaattcat tttttcttaa ggcgtacaca gaaatcaagg
    18901 aagaaatagc caagaagata aagaaatagt aagataaaaa aagatgtcaa aataactagg
    18961 tgatgaaatg aagcgaaaac gattatctat tatccgtatt cttttttaca tgatcattct
    19021 gacggttatt ggtgcggtgt tgtttttagg aggaagaact tatcgttcgg taaaacgagt
    19081 ggaaagctat caaatagaaa taacaacagc tgcaaaaaat tatcatatgg aaaactacga
    19141 gaatttgatc aaggcgataa tattgaccga atcaaaagga aaagggacag atctcatgca
    19201 gagctcggaa agtctgcatg gagaagctga gatcattgat aatccagctg acagtattga
    19261 tcaaggtgtc aaatatttgg cggaaatgat agtagaagca aagaaacaag gatgcgattt
    19321 atggacagct gtccaagcat ataattttgg gaaagattat attgcctatg taaaaaatca
    19381 tggcggagaa aacacagtag aattagcaga agcgtattct aaagatgtgc tgagcccact
    19441 tttaggaaat gaggaaggga aacaataccg atattggcgg atccagtcct tgctttacaa
    19501 tggcggctat ctctatcata atggaggtaa catgttttat cctgatattg tcaaaatgaa
    19561 tgaaaaaata atagagacct atgaaaaagt ctttaagtaa aatagcagga aatgttcaat
    19621 catgaataaa attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg caaaaaatgg
    19681 aaaatcaaga agtggggtac acaaccaaag actggatgtt acggtttgtg aagggaatgt
    19741 ttatcggttc aggatttatc ttgccaggag tcagcggtgg tgcattagca gctgtctttg
    19801 gcatgtatga gcggctgatt tcttttttag cccaccttac aaaaaatttc aaagaaaatg
    19861 ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg catctttcta ctttcttttt
    19921 tagtcagttt tttattaggt gcgtatgaaa caacgatatt atggtttttt gtcggatgta
    19981 tcgctggaac tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga gatacttctg
    20041 atgtgatcat catggttgtt acttttgttc tggcttattt gtttttatta tacggcgctc
    20101 aattatttga cgggcaagtc gatcagaatt ttggaacatg gctgattgcc ggcggattga
    20161 ttggtttggg tatgatcgtc ccaggattga gcccttctaa ttttttggtg tatatgggat
    20221 tgtataaagc gatggctgac ggatttaaag aagtacgact tgaagtgatt gtgccgatcg
    20281 gaattggtgg gattctttgt gttcttggat tgtcaaaaat catggattac attttctcaa
    20341 aggcatatag caagctattc catttcattt taggtgtagt ttttgcttca acgattatga
    20401 tcattcctac cgattacgcg ggattgggag ttgctggggt actagcttgt cctgttcttt
    20461 ttatagccgg tacagcacta ggttggtgga tgagccgttt ggaggaacaa tataagtaaa
    20521 gatagattga taataaaaac agcacacggg tgattcaaac aaaaaggttg gcatcttttt
    20581 tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt tttaatcttg
    20641 gaacacgcta gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat ggagaaaaag
    20701 aaaatgaatg gacttacaca gcaagaggta gttcagaaaa tggcttctgg ccagcaaaat
    20761 gactaccagg aagatgcatc aaaaagtaca aaacaaatat tcagcgataa tatattgacg
    20821 ttattcaatt ttctgaactt tggtattggg atttgtctgt tatttgtagg tgcgtattcc
    20881 aacatggcgt atttagcaat tattgtcgtc aatatcgtta ttggaattta tcaagaaata
    20941 catgcaagaa atcttgtacg gaaattatcg attgtttccc aatcagaagt actagtgatc
    21001 cgggacggta aagaacaatc gatttctact aaagagctgg tattaggaga tttggtaaaa
    21061 atcggagcag gagaacagat accagccgat ttacaggtta tatctggacg ggcagaagca
    21121 aatgaagctc ttttgacagg cgaatctgat ttgattgtca aagaaactgg agctgaattg
    21181 ctttctggaa gctttttgac aagcggttct gtcatggctg aagttatccg agtaggtgca
    21241 gataattatg ctgtccgctt gactagcgag gcaaagactc acaaagctat tcgttctgaa
    21301 cttgtagatt cgattcgcaa agtatccaaa ttcactagtt tcgtgattat tcctttgggg
    21361 atcatactat ttttggaagc actgttcatc cgcgatgctt ctatcaaaat ttctgttatt
    21421 gcatctgcag cagctttgtt aggcatgctt ccaaagggtt tggtattgct aataagtatt
    21481 gcattaacga caggggtaac caaactggca aaaaaacgaa tccttgttca agacatgtat
    21541 tcaattgaaa cgctggccca tgtcgataca ttatgcttgg ataaaacagg gaccattact
    21601 gaagggaaaa tgagtatcca gcgggtagaa gcactacatc cgctgtacga gaaagccatc
    21661 ccagaaatta tggggacgta tctggcagaa agcagtgata acaacatcac tatgcgggct
    21721 atgagagatt attttgagct ttctaatcgg tatgatgtga caaacaaagt cgcattttct
    21781 tctgatcgca aatggggcgc gatggaattt ccagaactgg gagtcgtgta cgtaggagcg
    21841 cctgaacgtt tggtggaaga agatcggctt ccggacagtc taaaaacagc ccaagcgaat
    21901 ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc aattgaacga agaaaaacca
    21961 gcatttgttg agccggttgc tctgtttgaa atcgatgatc caatccgaaa aaacgctcaa
    22021 gaaacactag cttatcttca tgaggaaggt gtggatctga aagtgatttc tggagacaat
    22081 ccttttactg tctccaatat cgctagacgt gcaggattac cgaattatga tgcgtatgtc
    22141 gatctttctc aaattactga agaaatggaa gtgcgagaag ctgctcatcg ctatacggtt
    22201 tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa agaaagcggc
    22261 agaacggttg caatgacagg cgacggtgtg aatgatgttt tggcgttaag agaagcagat
    22321 tgcagcattg ctatggcaga aggtgatgga gcaacacgtc agattgcgaa tctggtattg
    22381 ttggactctg attttactac attgccagaa gtcctgtttg aaggacgccg agtggtcaac
    22441 aatgtgacga aagtctcagg catctttttt atcaaaacga tttattcatt tattttatcg
    22501 attatatgtg ccgttacagc aattggattt ccgtttatcc ctatccaaat cacgctgatt
    22561 gatttagcga ttgaagggta tccttccttc tttttatctt ttgaacaaga taaaagaaaa
    22621 atcacttacc gttatttgcc tacagcactt attaatgctt taccaaatgc cttgcttgta
    22681 gtagtgaata tcattgcggt gtatctactt ggaaaaacat tcgcatgggg aaatgctgaa
    22741 actactactt tgatgtatta cttgctgatt gggatcagtt gcatggcagt tgtaaaagct
    22801 tgttatccct tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt agggatatat
    22861 gtagctgcaa tgctgttcca tggaattttg gaagtaaact tagggttggg agagagcgta
    22921 ggatacttct ttgtattcat gctcgtaaat attgttcttc gagtattgta ctggaaggct
    22981 gatattcaaa atcagttact gaaaagagta gcaaattgat gcttaatgag atagaaatgt
    23041 gttatacttg aaaaagcaat gaaatatgat acaatcaaac atgtataatt gtgtccttta
    23101 aaatcgcact gatttcagtt agtgcgattt tttaccggtt attctcctga aatatcttct
    23161 gttaaaccca aaagatatca atagagcagg gggtgaaaaa atggctcaac ggattcctca
    23221 agaggttatc gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg gacaatatgt
    23281 ccagttgaaa aagtctggta aaaattactt ggggctgtgt ccttttcatg aagaacgtac
    23341 accgtctttt tctgttgcag aagacaagca gatttttcat tgctttggtt gtggtaaagg
    23401 cggaaacgtg tttacttttc ttcaagaact agaaggtcta agttttccag aagcagtggt
    23461 aaaagtggca gacttcgaac agataccgtt agaagatcaa tggcgtcagc gtactatagc
    23521 tgtccaaaat cctgaatctg ctacggggaa actgattgct gctcatgaaa aagcagcgga
    23581 aatctatcat cacatgctat tgcatacaaa agctggacag ccagctttag aatatttgca
    23641 aagtcgtggg ttgtctttag aattaattga agaattcaac ataggttttg cacctaatga
    23701 gcgttctttt cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat tgttagaacg
    23761 ctctggattg tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt atcaaagagt
    23821 gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac gctggcttgc
    23881 acctgaaaaa gggcaggaaa aagatcagcc caaatacttg aatagtccag aaacagaact
    23941 gttcaataaa agacaagttt tatttaatct tgataaagca agaagcgata tccggaaaaa
    24001 cggagaaatt ttgttgttcg aagggtttat ggatgtgatt gctgcatggc aggctggtat
    24061 caaaactgga ttagcttcta tggggacaag tctaacagct caacagatcc aacaaatgga
    24121 aaagctgacg aaagaattgg tcttttgcta tgacggagac aacgcaggtt ttgaagcaac
    24181 caatcgaggg attgaattgc ttcggcaaaa cagtcgatta gcactttctg tcgtagtcgt
    24241 tccagaaaag cttgatcctg atgaatatct ccgtaagtat ggagaagcat ctttccaaga
    24301 attagtggct catgggcgcg aaacagtctt tacttttaaa tcacgttatt taaaacaagg
    24361 gaaaaattta gataatgaaa aagacaagat cgaatacctc gaagaattac tagttgaact
    24421 tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt caacagagtt
    24481 tcagatcggc agagagacga tacaaaaaca actgcgcgat cttcgaagga caaatcagca
    24541 aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt acgagacaag aaactgcacg
    24601 gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag attctattat atcgtgcatt
    24661 taaagaagtt tctgtccgta atattttaaa agaacgagaa tttcagttta ttcatgatgc
    24721 atatgcagaa gtttattttt tattcgatgc atatgtctca caacattatg aattcaacct
    24781 tgcagagttt ttagactttt tgcaagatga aaatttaagg cggttggtgg tggaaatcga
    24841 atatttaccg gtggcagaag aaagttcgcc acgagaaatc caagacttgc tagatgtgat
    24901 cagcaaatct ggcttagctg atgaaataac attaaaaaaa caaatgcagc aagaagccag
    24961 acgaactgga aacaaacagc ttgagcttga attgacgatt gaaatcatca atttaacgaa
    25021 acagttaaaa caagccaaat agctactcaa ggggggcctt cttttatggc aaatggaaca
    25081 gataaaaaaa cgtatgaagc agcagtagca gctttcatca aagaaaacaa acccaaacga
    25141 caagttgtat atgatgattt atccaatcag cttgctacac cattcacttt gaatgcagat
    25201 gaaatggaca aattgatcca aaagattgaa gacgcaggta tcagtgtggt ggatgaaaat
    25261 ggtgatccat caattcacag cttgaagagt gctgagaaaa aagctgaaca agcaaaaaca
    25321 gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat gtatctgaaa
    25381 gaaattggcc gtgtctccct tttaactgcg gaagaagaag tagcgttagc attgaaaatc
    25441 gaagaaggcg accaagaagc gaaacagcgt ctggcagaag caaaccttcg tttagtagta
    25501 agtatcgcta aacgttatgt aggacgaggc atgcagttcc ttgacttgat ccaagaagga
    25561 aacatgggac tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt taagttttca
    25621 acctatgcta catggtggat tcgtcaagcc atcactcgag caatcgctga ccaagcacgt
    25681 acgatccgta ttccagttca tatggttgaa acaattaata aattgatacg tatccaacgt
    25741 caattgctgc aagaccttgg aagagaacct acgccagaag aaatcggtgc ggaaatggat
    25801 cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt ttcccttgaa
    25861 acgccaatcg gtgaagaaga tgactctcac ttaggtgatt tcatagaaga ccaggaagca
    25921 acaagtccag cagaacacgc agcttatgag ttactaaaag aacaattgga agatgtacta
    25981 gatacattga ctgatcggga agagaatgtc cttcgcttac gttttggttt agatgatggc
    26041 cggacgagaa cattggaaga agtagggaaa gtgtttggtg ttactcggga acgtattcgt
    26101 cagatcgagg ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa acaattaaaa
    26161 gatttcttag aataagcgtg gactattgac cagaagagaa atcagtaaaa ctgtttggtt
    26221 tattttaaaa gaaaaaaagc ttgagactac gcagtctcaa gctttttttt ctataggaag
    26281 gaaattcatt cattattttt aagtaaatcc taaaaataag ctattggcgt gacgtataga
    26341 atatgataga ttaaatggta gcgtaaacct agtaaaggag ccgatcgcta agtgatgaaa
    26401 aaatgtccaa attgcggatc gaaaaaaatg gagcagaata ctatctgtcc agactgcggt
    26461 catcaaataa ctgcagaaga tcagcaaata aaagaatcaa ttgaagaaac aaatacagat
    26521 gcagctatgc aggataaatc aactgaagat gcagtctttc ctgatacaga attaaacgat
    26581 ccaatcgaat ggtcggaatt gaaagattta cctctagaat ctgtaatgga attattcgaa
    26641 aaagcagaac cagaagacga agaaccacct gctcacgatc aagtaactaa tatagagaaa
    26701 aatgaacaga agaaagcaac tgttcatgag acttcggaag aatcaggaaa gaatcaagaa
    26761 gaaacgcttg aatcaaaaca aaaaagaaga gtagctgaac tgaaagaaat cgtcgataat
    26821 gaggaagaaa attcgatact atccgcttat atcaaagcac atcgtgaaga tacaaaagaa
    26881 gagcacgcga aagagctgct caaaatgatc agtgaaaaaa ttgcacagga aaacaaagta
    26941 gagccagaaa aggaacaaga aactgaaaaa gactctgata gagaaaacgc cacaggtaaa
    27001 gaaacagtaa aaataaaaga cgtaaaaacg gatatcacag aaaaaactga agtatttact
    27061 gaaaaacaat tagaaaagga aaaaacgaat aaactgccgg aagcagagaa aaaagaaata
    27121 ctcgaaaaca cagacaaaaa gtcaacagag cccgatgaaa taaatagtga atccgatgct
    27181 gaaaatcaat ctgtaagcaa agagggtact gatcttgaag caaaaataga aaaagaagag
    27241 aaccagccta agctgtttga agcagaagcg aaatcaataa caatcgtgcc agatactgaa
    27301 aaaacacatc aagaatcaaa cttagaaaaa acagatccag ataaagcgca aaagaaaaaa
    27361 tcaaaaaaag taccttatct cgttttagca gcagtacttc tactcggtgg aggaagctgg
    27421 gcttattatg accatcatca aaaagtagaa gccgaagtag cagcagaagc aaaaagacag
    27481 caacaaatgg aagaacttca tgagaaactt gctgcctttt atacagatga tcaccatcaa
    27541 tttattcgtg cagatatgat tcatcaagat ttatcaaaac taaaaaatga tttatccagt
    27601 cttaagaatg aaaaaggata ttcagagtta gaaacaactt accaagatat ccaatctaag
    27661 atccgagata tccaaaaagt gaatgagtgg ttcatagcac ctgccatcgt cgatgatcat
    27721 ctagcagatg acatcaagtt gaaagcagat aaagagatcc aagcaatcga aacggataac
    27781 acagattttg gaaaattgat tcaacaagca caaacagaag ctaaaaatca atatgctgaa
    27841 ttgcaaacag ctaaagaaaa aacagctata gtctttaaag acggaaaagt agtggattca
    27901 gcaacacgcg accaatacaa gacggcaaaa gaagcagtag aaaaagtgaa aaatacagat
    27961 ctatctgctc agttgaatga acaattgaaa caagtagata agatgctcac tgataaagag
    28021 aaaaaagaag aagaggaaaa gaaaaaagca gaagaagcga aaaaagcagc tgaagaacaa
    28081 gcaaaacaag ccgctcaagc agcagctgca gctcaagccg ctcaacaagc tgcggtcgca
    28141 caagcagcac agcaagaagc ggcatcctcg accccttcct cttcaaactc agcgaatcag
    28201 ccaatcaaat ctgccagagc aagtgatatt gccgatgcat ctaatccagc atggacgtgg
    28261 gcaccaggtg tccaaagcaa agtgattgct acatgtatcc aacgtggata tatcgttgaa
    28321 ggaggttaca gattagaaaa agccagaatc gaaaatgggg aaggctatta taatctatat
    28381 gccacttcaa caaaatctgc attgatgaaa ggcattggag aaagtgcttt gccattttac
    28441 atcgtaacca tcaactgtaa aactggatgg ttcggaggaa atgggagcaa ttaattttag
    28501 gcaaatgaaa gaggctgtga gcctgccgtt taacaccgtt tattcgaaaa aggggagtat
    28561 gacacaactg ttgtcatact ccttttttca cctttccaaa ccaaggctca gctattcaag
    28621 tagataacat tatgttatga tagcaaagaa atgtattaag aaaacctttc actatagagg
    28681 gagaaaaaaa tgaacgattt attagcaact atatttacag gtattattat tgatgaaaat
    28741 gaaaaaaact actttgtcca aaaaaacgga caaacctttg gcttaagcaa atcagaaggt
    28801 gaacacaaaa tcggtgaagc agttgaagga ttcggttacc aaaatcaaaa acatgaaaat
    28861 cggatcacta cacaaattcc taaaagccgt atcggtcatt acgcttttgg aatcgttact
    28921 gctacacgaa aagatttagg tgcgtttgtc gatatcggtc ttccagataa agactttgtc
    28981 gtatctcttg atgaattgcc gacaatgaca gagctatggc ctaaaaaagg ggatcgcttg
    29041 atgatttccc ttagagttga tgcaaaagac cgtatttggg gcagtttagc agatgaaaaa
    29101 atattcaaat ctttaagcaa atatggaaca gaagaaatga aaaacaaaga tgtgatagga
    29161 acagcatacc gcttgaaatt aacaggtacg tacgttttaa cggaagattt ctatcttggg
    29221 tttatccatc cttctgaacg atataatgaa cctcgtctag gcgagcagat aaaaggtcga
    29281 gtgattggtg tccgaccaga cggtgtactg aatctttcat tgaagccaag agcatatgaa
    29341 gctatatcag atgatgcaca aatgatttta accattttag aacgttctgc agatcagcaa
    29401 atcaacttta cagataaatc ggatccagaa gaaatcatga aagcttttgg tatcagcaaa
    29461 ggagcattta agcgcgcatt aggtaatctt ttgaaacaag gattgattac acaagaaaac
    29521 ggtgtgacaa aactagtcaa aaaatctaat tgagaatccc ttgcatgttt ttcttaacta
    29581 gcttataata attttaatta acatagtgtt atatcttttt ttatttgtaa ttattataaa
    29641 ttgttggggt tgagtaaatg gtcatgaatg ctatttcggc gatgaaaaaa acaaaacagc
    29701 agctacatga agcggggttt aagctgacac cgcaaagaga agctactctg cttgtgcttt
    29761 tggaaaatga gaaagagcat ttatctgcag aagaaatctt ctttttagtg aaaaaaaaga
    29821 gtccggaaat tggacttgct actgtttatc gaacccttga aatcttgaca gaattgaaaa
    29881 ttgtcgataa agtaagtttt aatgatggat tagcccgtta tgatttgaga aaagaaggcg
    29941 ccaaacattt ccatcatcat ttactatgct tgaactgcgg cacgatcgag gaagtagaag
    30001 aggatctttt acttgaagtg gagcaagtag tagaagaacg ttatcatttt ttagtaaaag
    30061 atcatcggct aacgttccat ggaatctgtc aggaatgcta tgaaaaagaa aaaagaaacc
    30121 agtccgtctg agtgtctaga cggctttttc tttgctcaga ttatggaaaa agactatcag
    30181 aatgaagcgg tatctgatat gatagagagg catgaggggg attattatga aagagcaaat
    30241 caacgattat ctccactact tgaatattga acggggattg tccgccaata cacgacaaag
    30301 ctatgaacgt gatttggaac agtacttgca ttttctacag gagcagaaaa ttgcttcatg
    30361 gcaggatgtc gatcgctttt tagtcatccg ttttctggag aagttgcatg aagaaaaaaa
    30421 agcgacagca actgttactc ggatgatctc aagtctcaga agattccatc agtttttacg
    30481 gcaagagcgt atcactgatc atgatccgat gcaacatatc gatacgccga agaaagcaca
    30541 gaaattacca agtacattga ctctgacaga agtagaaaag ctgattgaag cacctgatac
    30601 gacaaaaacg ttagggataa gagatcgtgc aatcttggaa gtcatgtatg ctactgggat
    30661 gcgcgtcagt gaattgattg gattgaaatt gggagatctt catctttctc ttggactggt
    30721 acagacagtt ggaaaaggag ataaagagcg gatcattcct ttaggtgatt atgcgatcca
    30781 atggctggaa cgctacttag acgaagcacg tccattactt gtaaaggacg catctgaaat
    30841 gcatgtattt gtcaataatc atgggaaagg tctttctcgg caaggaatct ggaaaaatct
    30901 gaagcagtta gtacgagagg ctgggattta caaagaagta acaccacata cgctgcggca
    30961 tagctttgcc acacatctgc tagaaaatgg agctgatttg cggacagtac aggaattgtt
    31021 aggtcacgcg gatatctcta cgactcagat ttatacacat atcacgaaga aaagaatgac
    31081 ggatgtttat aagcagcatt ttccaagggc ttagtcatag gaaggtgaga agatgttaac
    31141 acattaccgg aaagaaaacc gtaaaattgc tatgggacta ttgagtttcc ataagaaact
    31201 gacaggtaaa acttctttgt tgaaggagat tgatacgtac gaatctgaag acgattatga
    31261 actttttttc tttacgccag aaggttcaac caacattcaa gggattattg gtatttactg
    31321 ggcaacgaac gatcagctga tcatccatga catttctttg aatccgtctt atcgcggtga
    31381 gaatatgggc tttcaaatgt tagatgaatt acaggaaaag tacccggatg tctccatccg
    31441 tgcaactgaa atcacagagc cttacattgg aaaatggcta gcaaagaaaa agaagaaaga
    31501 gtgaatgagt tgacggagat caatattaaa ttagatgtat tcgagggtcc tctcgatctg
    31561 ctgcttcatc tgattcagca aatggaaatc gatatatatg atataccgat cgctacgatc
    31621 acggagcaat atatgaatta tatccataca atgaaaacac tcgagctagc tgttgcagga
    31681 gaatacttag tgatggctgc tacattgatg gcaatcaaaa gcaagacatt gcttcctgtc
    31741 caagaattcg tcacagacga tgaagaatta tgggaagaag atcctcgaga agaattggtc
    31801 actcagcttt tagaataccg taaatataaa tatgcagctg aaagactgac agaaaaagca
    31861 gaggaacgaa gtctttatta cacaaaagaa cctatgaaca tcgatgggtt agcggatgct
    31921 gaaaagccgt tgaagcgagg acaagtcaaa aaaatcgatt tgatccgtgc catgcagaaa
    31981 atcttagaac gtaagaaatt ttctcaaaaa gtggaaaaaa cgatcacgcc cgatgacaca
    32041 acgatcgaag aacgaatggt atttatcgaa gagcgtataa aaagaaatgc gcatggctgt
    32101 actttcgatt ctttttttga gtcaccttct aaaagtgaag ttgtcactac ttttatggct
    32161 ttgctagaat tgatgaaaaa agggcagatc attgcgcaac aaaaagaaaa ctatgaatca
    32221 attatgcttt atccagcagt aggagatacc gaacatgacg aaattaagtg aacttgaagc
    32281 ccttcttttt attgttggcg aggaagggat cagtaatgaa gaattaagcg gactgttaaa
    32341 tctcagcgaa gcagaagtca caagtttagt gaaagaatta aaagaatctt acactcaaaa
    32401 ctcgcactca gctttgcata tttttgaaac ggaaagccat tatgtattaa cgactaaaaa
    32461 agagctggct cccttactta agcgatatgc gcaagggaca tcaaacacat tatctcaagc
    32521 cgccgtcgaa accttagcaa tcatcgctta taaacagcca atcacaagag ctgaaatcga
    32581 gcagattcga ggagttcagg tttcaggagc ggtacagcgt ctagctgccc ataaactgat
    32641 tgaagaaaaa ggtagagtag aaggaccagg tcggccgata ttatatggaa caactggcta
    32701 cttcttagat tattttggtt tgaaatcatt agaagaactg cctgatacac aagaaatgga
    32761 aatttcactt gaagaagacg tgtcaccaga tctattcttt gatggaatga ataaaggaga
    32821 ataattgtaa tggagagatt gcagaaagta attgcccacg ctggcatcac ttctcgtcga
    32881 aaagcagaag aattcatcct tgcaggacgt gtaaaagtaa acggacaagt tgtaagggaa
    32941 ttagggataa aagttgggaa acatgacact gtggaagtgg atgaagtccc aatttatcaa
    33001 gaagaatacg gttactattt attgtacaaa cctaaaggag ttatttctgc tgttgcagat
    33061 gacaaaggaa gaaaagttgt tacggatctt ttgccgatgg taaaagaacg tatctatcct
    33121 gtaggtcgac tggattacga tacttcgggg atcttgctac tgacaaatga tggagatttt
    33181 gctcagcgcc tgacacatcc aaaacatgaa gtggataaag tttacgttgc aaaggtaaaa
    33241 gggatcgcta caaaatcgat gcttgcacct ttgacaaaag gaataaagat cgaaggacga
    33301 aaaacatctc cagctaaatt ccaaatttta tcagtggatc ctaagacaaa ccacagtatc
    33361 gtggaattga cgatccacga aggacgtaat catcaagtga aaaatatgct tcaagcagtt
    33421 ggtcttcctg tgcaaaaact aaaacgtgaa aaatacggag atctgacttt acaaggccta
    33481 cgtcctgggg attaccgtga actgaacaaa aaagaagtca gcagtttgct gaaccactca
    33541 aaatgaacac ttacttttta taagtgttag atgttgcgtt ttttgaatag atatgtataa
    33601 tgaatatgta aacaaaaatt atataaggtt cgtcttcagg ggcagggtga aattcccgac
    33661 cggtggttat agtccacgac ctattttgca aaaaaatagc tgaatcggtg aaattccgat
    33721 accgacagta tagtctggat aaagaagata gagcttattt gacgacgctt gatcaaataa
    33781 ctctaactct attttccctg gaaaatagag ttttttcatt tggagagaaa cgtcaaaaag
    33841 ttcgctgaag aggagtccta acaggaggat ttcaaatgaa gaacacccgt gtacagaaaa
    33901 tggttgcagt agcgatgttt gctgcgatcg cattagtttt acagtatttt gcttttccga
    33961 tcatgccggc ttttagtttt ttgaagatcg attttagtga tatctcggta ttgatcagca
    34021 tgttcttatt cggtccagcg gccggagttg caacagcgtt cttgcgttca gtattacatc
    34081 tgctgactac aggtttttct ccggataatt tggtaggaga cactgctagt ttcttatcaa
    34141 caacgatctt tacattcccg atttattatt tctttagtgt aaagaaacag cataaaggaa
    34201 gaaacaaatt tttaggtgtc attacaggaa ccatcgctct aactattttt atgagtatcg
    34261 caaattattt cgttattacg cctctttact taatgttttt agggctgaac gcaacacaga
    34321 tgctgggcat gtctttagca aactatgtac tgattgggat cgttcctttc aacttgatca
    34381 aaggattcat cgtgagtgct gtctttttag tgctgcatgc gcaacttctt ccatggttaa
    34441 gtcgaaaaca acatcagttc gaacaacgtc atacagcagt caaataaata tcaaaaagat
    34501 caagaaattt cttggtcttt ttttttggaa aaaaatacaa gacatgcaat aatgtaaacg
    34561 ttttatttgt ttgttttgag tatttttttg atttatgcat aaaaatcaat caattctttg
    34621 caaatgtgaa attcttctcc ttttaaaaga cgaaaatgtt tgtttataaa aaaaaggaaa
    34681 cgctacaatg aaaaagaaga aagctttaaa aacctgaaaa aagaggaggg aatagggaaa
    34741 aacgaacttt ttgaacaaaa gttttacaaa atgcgtgtta ttatttttgt tctgatactc
    34801 tttttgataa aataacatta gagccaaaat tgttgtctct aacggaaaag aaaggaagaa
    34861 gaagatggaa caaacaaaaa agaaaaaatt tcaaatgcct tcagcgtata cggtcttatt
    34921 tattattatt gcagtaatcg ctgtacttac ttggtttata ccagctgggc actatagtgt
    34981 agacgatgca ggcaacatcg tcgctggatc atatgaacga gtacaacaaa atcctcaagg
    35041 gctgtgggat attttcatgg cgccaatcaa tgggatgcta ggtgtaccag cgggggagct
    35101 aactaaagaa acacctgctg caatcccaat ctctttcttc attttggttg ttggtggatt
    35161 tttaggtatt atcaacaaaa caggggcact agatgccggg attgcttcag ttgtgaagaa
    35221 attcaaagga aaagaaaaaa tgcttattcc agtattgatg ctgttatttg cattaggcgg
    35281 ctctacttac ggaatggcag aagaaacgat tccattctat gctttactga tcccagtcat
    35341 gatggctgta ggctttgata cagtagtagc tgtagcaatc gtattagtag ggtctcaagt
    35401 aggctgtcta gcttcaacgg taaatccttt tgcaacaggc gtagcttcac aagctgttgg
    35461 gatttcgatg ggtgaaggta ttggtcttcg attcttgatg ttcgttattt tgttagctat
    35521 ttctattgta tatgtgtatc gttatgcatc aaagatcgaa aaagatccaa caaaatcatt
    35581 agtatacaac caacgtaaag aagatatgaa gcatttcgat attgaagcaa tcggtcgtca
    35641 agaagtaatc acgaaaaaac aaaaacatgt taatgtattg ttctttatta cttttggtat
    35701 catgatcatc agtttgattc cttggacaac cttgaatgcg aatttcacga tttttgaatc
    35761 attgacaaac tggttaacaa accttccggt actaggtacg gttctaggta aaagcatgct
    35821 tccattaggt gactggtact tcccagaaat tactatgtta ttcttagtaa tgtccatcgt
    35881 agtagcaatc acttatggca tgaaagagtc tgaatttgtc tctgaatttt tagctggtgc
    35941 ttctgatttg atcggtgtag caatcgtagt agctgttgct cgtggtatcc aagtggttat
    36001 gaacaatgga ttaatcacag acaccatctt acattggggc gaacaaggat taagttcatt
    36061 atcttcaagt gtattcatca ttattacatt tattttctat atccctatgt cattcttgat
    36121 tccttcaact tccggcttag ctgctgcaac aatgggaatc atgggaccta tgggtgaatt
    36181 tgccggcgtc ggtaaggatt tagtaatcac tgcttatcaa tctgcatctg gtttagtaaa
    36241 cttaattaca ccaacatctg ctatcgtaat gggtgctgta gcaattgctc gtttcgatgt
    36301 ttcagtgtgg tggaaattta ccggtaaatt aattgcgata ttatttgtag ccatctgtgt
    36361 gatcctaggt gtagcagcaa tgttctaaac ctagacaaat acaggtacaa ggtctatcat
    36421 aagaatgaag aaaccgataa acggctatcg ataagcacgt ggataagtca atagctccat
    36481 tcagtgagtg ttttttcttt catcatttgt taagaacatt ctgcttgctg atggagcagg
    36541 cttaaaacgt cattatttga gaagaagagg ttgtgtcgga ggaatttgac atgacccttt
    36601 ttaacatatt aggaggataa tatcatgaaa aaatttgtga cagaacaaca tcatgaacaa
    36661 gcattagaat cattgagcga attgatccga attccttccg tactagatga agcagattca
    36721 ggacaaggac atcccttcgg taaaaaagtg attgaagctt tggataaagt attggaaatc
    36781 agtgaaaagc taggatttag aacatttaaa gatccagaag gctattacgg ttattcagag
    36841 attggctctg gagatgaatt attcggtatc ttgtgtcata tggacgttgt tccagcaggg
    36901 gacgaaaata attgggaaac aaaaccattc gatccaacta taaaagacgg ctggttagtt
    36961 ggtcgcggat cccaagatga caaaggtcct tctattgcgg caatgtatgc cgtcaaagct
    37021 ttgattgatg ctggcgtgga attcaagaca cgtattcgtt ttatctttgg tacagatgaa
    37081 gaaaatttat ggcgttgttt agaaaaatat aacgaaaaag aagaaggaat tacgcaagga
    37141 tttgcgccag atgctgaatt tcctttaatt tatgcagaaa aaggcttatt gcaagcttac
    37201 ttgacaggac caggaacgaa cgagttttct gtaaaagctg gaggagcgtt gaatgttgtt
    37261 cctgatgcag ctccttactc tggagaaaag ctttctgaag taaaagaagc attgaaaaaa
    37321 cacgaattcg atttcgaaga acaaggtgaa ggcatcgtcg ttcttggtaa aagtatccac
    37381 gcgaaagatg cagcacaagg agtgaatgct atttctcgtc tggcaattgc tctttcagaa
    37441 gtatttgatt ttggcccaat caatttctta ggaaaattag ttcaagaaaa tgcaactggt
    37501 gaagctgtcg tgggcaaaac agaagatgaa caatccggcg agctaacgat gaactttgca
    37561 agtttggaaa tcacaccaga acaaacaaaa atcggtgtag atatgagaat ccctgttaca
    37621 ttcaagaaag atgatttagt agctaaatta actaaaacag cagaaaaata tgggttgact
    37681 tatgaagaat ttgacttcct tgattcatta tacgtgccac tagacagtga gttagtgaaa
    37741 aatctattgg ggacatatcg cgatattact ggagacatga ctgaaccgtt tgtatcaggc
    37801 ggcgctactt ttgctcgtac gatgaaccag tgtgtggcat tcggtgcgat gttccctgat
    37861 actcctgact ttatgcacca ggcgaatgaa cgatgggaat taagtagtat gtataaagca
    37921 atggaaattt atgcagaagc tgtgtatcgt ttatgtgcta aataaaaata aacatacaaa
    37981 aagggcggat attcaaatgg atattccgtc tttttttatt tgatgctgga aagtttgcat
    38041 ttctctctta tttttttaag gtataatcat acttgattaa gaaaaagggg tcactcagat
    38101 agaaaaggtg ggagaaagtg aaaatattta gacgaatact aattggctta gtcatcattt
    38161 taggattact cggtggagcc ggttattact atatacagca aaatacgtac gaaccatcgt
    38221 cagaagcgca agtgttggta aaagaaggaa aaaacaaaga agattactta ttttttgaag
    38281 gagagaaaaa gaatcatgga aagtctccat tacttatttt ttatcctggt gctctcgtag
    38341 agccagaaag ttatagtatt tgggcgcaag atttagcaaa aaacggcttt tcagtcgcta
    38401 ttgtcaaaat gccacttgat ctagcagtga tgggcggaaa caaagcggat aaggtcaaag
    38461 aagattttcc aaattccgat tatgtgatcg gtggccattc attaggtggc gtaatggcaa
    38521 gtcgttatgt caataagaat cattcagaca aacatttaaa aggggtattc ttcatggcaa
    38581 gttatcccga caaaaaagga acattgacag aatcagctgt tcctgtttta tcgatcacgg
    38641 cgacaaaaga taaagtattg aatcaggaag cttatcaaga agctaagcag tatttaccgg
    38701 aaagaacaga gtatgtatca atcaatggcg gaaatcatgg aggttttggc agctatggtg
    38761 agcaaaaagg agatggagag gcagaaattt ctaacgcaga ccagcaaact gtgatcgtca
    38821 atacattgga aaactggctg aaaaagttga aataatataa atgggtccta tagggtccgt
    38881 gaaaaaacta gtcaattaac tatagatagc aaatgaatct ttcaaaataa gtcgggattt
    38941 cacggaacaa gagaaacaga tcccggctta ttttcaagtt tagagaactt agtgtacgtt
    39001 tttaaagttt cgtttatatt tatattttat tttctaataa aatcgctcga actggacatt
    39061 tttgatacgc tttttttaca tatgtttctt cttccggcat cacgaactgt tgatgagcct
    39121 ctggttcttg ggcaaataaa acgattcctt cttcatcata atcgaaaata tctggcgctt
    39181 cgatttgaca aagaccgcat gcaatacagc gttcaggtac aagtttacat agtagtgaca
    39241 tttctaacca tcctaactta aggagaataa tatgattgtt tttattttat cgctattttc
    39301 atcaggacac aagctgcgga ttagttctct ttaccaactc cttgtgggaa aacgaacgac
    39361 ttcagtactt atttatggat ttacccacga acttttgttt atccataaca gttttccaga
    39421 attgaagcag gataagtttt atcaaatttt gcaaaaaatt gcgcagcaag gttggatcga
    39481 aataaatgaa aatgaagcaa agctaacgcc tgcaggggct gatatgttgt ccgaacatca
    39541 aatagaacat actggtttgc gttttgaccg ctatggcaga acaggggaaa ctagttggcg
    39601 gctcattaaa tttgctgttc aagtaatttc taatctggca acgggtattc aagactatct
    39661 tccagcagag acaagtcctt tttatacttt ccaactaaaa aaatggctta gtgaaagcca
    39721 attgccacgt gaaattttga ttgatactgc gtatgagagt ttggtgcagc tgttttcgga
    39781 gataccagaa gaagcagcag attttttagc taaccaatta tctggaaatg agcgaacagg
    39841 gctgcttcct tatcagctgg caaaaaacaa tgatgagtca gcggtttatc tgcaacaaag
    39901 cagatgtatc caccttttgt tagcacaaat cgaaaaacgg ccagattcgc tttggcacgc
    39961 tttgatcgat tcattattac agcaaaattt caatcaaagc atgatgatca ccaagcagat
    40021 gtttatgaat ggtcagacaa ttgaccagat catggcaatc cgtcatttaa aaagaggaac
    40081 agtaacagat catcttattg agtgggcctt gttttttgat gattttccat acgagtggat
    40141 tctctctgca gaaacaatcg aacgtttgga accaaataag gattccgtgc gagagtggcg
    40201 ctttagcgaa tggaatgtag atggacaatt agattatgga gaatttcgtt tgtatcaaat
    40261 ctatctattg agaaaggaag cgatacagaa tgttaataaa tgaattaaaa aaatattttg
    40321 gatttgattc atttcgtccc ggccaagaag aaattattca ggcgttgctt gatggagaag
    40381 atactctcgc tattttgcct acaggaacgg gaaaaagcct ttgctatcaa ttgacggggt
    40441 atttaatgga aggtctggtc atcatcgtgt caccgctgct ttctctaatg gaagatcaag
    40501 tgacgcaatt acaaaaacga ggggagaaac gagtagctgc tttcaatagt ttactttcgc
    40561 aatctgaaag aagatacgtc ctccgtcatc tatcgcaata taaatttttg tttttgagtc
    40621 ccgaaatgtt gactaatcct tctcttttag agcatctgaa aaaacaaaat attgcgttat
    40681 atgtagtaga tgaggctcat tgcgtgtctc aatggggagt tgatttccgt ccagaatatc
    40741 aacagttagg aaaaatccga aagcttttag gaaacccggt cacactggca ctaacggcga
    40801 cagcaacaga tttagtagca agagatatac gtcatgtact atttgatcga ccaccaaaag
    40861 agatcagaca atcagtcaat cggcaaaata tctcgctttt tgttcggaaa acgcagcaaa
    40921 aagaacaaga acttgaacac ttcatggaaa gagcacaagg agcggcaatc atttattgtg
    40981 caacaaaaaa agaagtcgaa cgattgtatc atttattcag ggaacgtttt acagttggat
    41041 actatcacgg tggtcttgat gcagctcaaa gaagacaact tcaacagcag tttgtaaaaa
    41101 atcagctgca gtttctcatt gctacgaatg catttggtat ggggatcgat aaatctgata
    41161 tccgttatgt ggtccattat gatttgccag acagtctaga aaactatgta caagaaatcg
    41221 gtcgagcagg cagagatcag gaagaaagtg cagcaattct tttttatcaa agtggggatg
    41281 aacgtatcca ttatttcttt aatcaacttt ctagagaaca gcgacaaagt tttgagcttt
    41341 atttggaata tgccacagag caagctccct ttgatgagct gcaaaaaaaa tggatggagc
    41401 tgatccagca gtctgaaaaa ccagaaaatt gggtggaacg gctcaaacgt caggaaaaag
    41461 aaaaagaatt ccgcctgcag cagatgcttc gctatattaa tgaagaaaac tgccgcagaa
    41521 aattcatctt agcttatttt ggcgaaaaac tctccgaaaa gccgcagaat tgttgtgata
    41581 ttgatggagc aaaagcagaa cttctgccaa agaggaagct tttttctaag actgaacaac
    41641 ttcattggga aacgatttta ctaaatttat ttaaaaaaaa catggaacat tgatttttta
    41701 ggacaagaca ctttttccta tggtataata cacacgatag agtttaggag gtaaaacagt
    41761 gagcagaaaa gatagacatc aatctacaga aaataacgaa acacaagaac catgggaaca
    41821 accaatctac gatacagatg atgaagtttc atcaagaagt tctcagagac aacaaaagaa
    41881 aggaaataca ttatttcttt cgctattttt gattttactg gtcttatgta tcgctattcc
    41941 agcaggtgct tatttctgga tcagaaatgg atcaagcaac aatgcaacag cagcaacttc
    42001 ttctgaaact tcgacatcag tagtagaaag ttctacaagt acaacaaaag aggagtcaac
    42061 tgagacttca acagtagaat ctagtacaat cgcggaagaa tctactgtgt ctcaagatac
    42121 aggtgtaacg tcagaaagca gtattgcagc agagacaaca ccgacttcct caagtgaggc
    42181 aacttatcaa gcaggggatt caagcagcaa tgcagcagaa acaacacctt cttcatcagc
    42241 tgcagccggt tcaacaacga ctgttcaagc aggcgaaggt ccaaaacaag tggctgagcg
    42301 tgcagggatc accactgatc aattgtttga attgaatggc ttagatccaa acaactttat
    42361 gctttaccct ggtcaagaat taagaattaa ataataatag ccgcgctttg cggctttttt
    42421 tctgagaaaa aggagttata tcatgacaca aatcagtatt gcaatcgacg ggcctgcttc
    42481 atctgggaaa agtaccgttg ccaagatatt agcaaaagaa ttgaattata tttatacaga
    42541 taccggtgcc atgtatcgcg cagtaactta tttagctatt acgaacaatg tatctttttc
    42601 agatgaagaa gggctagttt cgctgatcaa aaagtatcct atctcttttt cgcaacaaga
    42661 agatggacag catgtattca tagcatcaaa agatgtgact ttagaaatcc gtcaaccaga
    42721 tgtcacaaaa gctgtttctg aagtctctgc acatggaaaa gtcagagaag aattagttgc
    42781 tgtccaacga aaaatagggg aacgcggtgg cgttgtcatg gacggaagag acatcgggac
    42841 agctgtatta ccgaatgcag aagtgaaaat ctttttagta gcaagcgtaa gtgagcgtgc
    42901 agaaagacgt ttcaaagaga accaagaaaa aggaattccg actgatcttg aaacactgac
    42961 gaaagagata caggaaagag accattatga ttcaacaagg gaagtctctc cgctgaagca
    43021 agcagaggat gctgttcgta tcgatacgac tggcaaatcg attccagaag tcgttgcagc
    43081 aataaaagct gttgttttga aaaaaggtta tcttcttttt taaattaaga aaaaaaatga
    43141 aaaacaattg ttttttcaag gaaaacgaac gaaatcgctt tatttttttt gaataatcaa
    43201 ttaaactaaa aacagtaccg tacttacggc agaatgttgg ttaggaggac aagtgttatg
    43261 acagaatttg atcaaaatca agagaacaac cagtcaatgg aagacgcaat gaaaagcgta
    43321 caagaagtaa aagttggcga tgttgttaag ggcgaagttt tagtcattga agacaaacaa
    43381 gtgattgtcg gaatcgaagg ggctggagtt gaaggagtcg ttccggcaaa agaattatca
    43441 actttgccgg tagaagatat cagcgaagta gtaaaagtcg gtgatgtatt agatttagtc
    43501 gttatcagta cgatcggcaa agataaagaa aacggcagct atctactttc aaaacgccgt
    43561 ttagatgcta aaaaagtttg ggaagatatc gaaagagatt tccaagaggg caaggtcatc
    43621 gaagcacctg ttacaaatgt tgtcaaaggc ggtttagtcg ttgacgtcgg tgtacgtggt
    43681 tttgtccctg catcaatggt ggaagatcat tttgttgcag acttttcaga ctataaaggc
    43741 aaaacattga gctttaaaat catcgaaatc gaaccatcag aaaatcgttt gatcttatcg
    43801 cataaagcgg tagtggaaaa cgaaagagaa gaaaagaaaa aagaaattct ttcaactatc
    43861 catgagggag atgttctcag tggacgtgtt gcacgtctta ctgattttgg cgcatttgtc
    43921 gacctaggcg gaattgacgg tctagtccat gtctcagaga tttcacatag tcatgtagca
    43981 aaaccaagtg atgtattggc tgtcaatgat gatgtaaatg tcaaagtgtt gtctatcaat
    44041 cctgaacaag aacgtgtttc cttgtcaatc aaagacacac agcctggacc ttggtcagaa
    44101 atcgatacaa aagctccagt tggcacagta ttagacggaa cagttaaacg tttgacaagc
    44161 ttcggtgcct ttgttgaagt gttccctgga gtagaagggc tagtccatat ctctcaaatt
    44221 tcacataaac atattgcgac accacatgaa gtgcttcatg aaggtgacga agtgaaagtg
    44281 aaagtattag agatccatcc agaagaacat cgtattgcat tgagtatcaa agctttagaa
    44341 gaaaaaccag cttcagaaga agaaccaaaa aatgttgagt cttatgaatt accagaagaa
    44401 aatactggat ttactatggg agacatttta ggtgatgcgt tgaaagaaga agtagaagaa
    44461 aaagcaaccg atgattctga aaaataattg attttgtatc tgtgactgag atgaaagcag
    44521 ctgcttttgt ctcagttttt cttttgaaaa gcgattagta tttcaataag gaataaaaat
    44581 acttcgttta attgacaatc tatgcttgaa agttggcaat gcattcgtta aactaagtga
    44641 gattggaaac ttgtgaggag gaaataatat ggcaaatcca acaattgcaa tcgttggccg
    44701 cccgaacgtc ggtaagtcta cgatttttaa ccgcatcgct ggtgagcgta tctcgattgt
    44761 cgaagatact cctggagtaa ctcgtgatcg tatttatgca aaaggtgaat ggttaggtcg
    44821 agaattcagc gtgatcgaca caggcggaat tgatcttggc gatgaacctt ttatggatca
    44881 gatcaagcat caagcagaaa tcgcgattga agaagcagat gtaatcattt gtgtagtcag
    44941 cggacgcgaa ggcgtgacag acgcagatga gatggttgcg aaaattttat acagaagtaa
    45001 taaaccagtg attttagcag tcaataaagt agataatcca gaaatgagaa acgatatcta
    45061 tgaattttat tccttagggc taggcgatcc ttatcctgtt tcaggaagcc atggactagg
    45121 gattggtgat attttagatg aagcagtgaa gtatttctct gaagagactg aagaagagga
    45181 cgacgacacg attaagttta gtttgattgg tcgcccaaat gttggtaaat cctctttgat
    45241 caatgcgatt ttaggagaag aacgtgtaat tgtttcggat attgaaggaa cgacccgaga
    45301 cgcaatcgac acccacttcg tttctgaaag tggtcagaaa ttcttaatga tagatacagc
    45361 tggtatgcgt aaacgcggaa aagtatacga aaatactgaa aaatacagcg tgatgcgtgc
    45421 aatgcgtgcc attgaccgtt ctgatatcgt tttgatggta ctgaatgctg aagaagggat
    45481 tcgggaacaa gacaagcgag tagcgggtta cgcccatgaa gctggtcgtg gaattattat
    45541 cgtcgtgaat aaatgggatc ttgtaaagaa agaaaccaat acaatgcgtg atttcgaaca
    45601 agagattcgt gatgaattcc ggtatcttga ttacgctcca atcgtttttg tttctgcagt
    45661 gactaaacaa cggttggaac gtctgcctga aatgatcgaa caagtaagta tgaaccagaa
    45721 tcttcgtatc tcttcggctg tgttgaatga tatcattatg gatgctgtag caatcaatcc
    45781 aacaccaaca gataaaggaa aacgcctgaa gattttttat gctacacaag tcgctgtcaa
    45841 accacctaca tttgtcgttt ttgtcaatga agaggaattg atgcattttt cttatgaacg
    45901 tttcttagaa aatcaaatca gaaaagcatt tacttttgaa gggacgtcca tccgaattat
    45961 ccctcgtagg agaaaatagg cattttacca tatcgacgtt tttttttcaa aagaaaaaga
    46021 cagtttcgtt tgttacgttt ctatgactaa aaaaagcatg tgaaaattgg taaaaacctt
    46081 gctattaagc ggttcatatg atatcgtgat aacagaatat tgaagttaat caatatttct
    46141 ttttaaactt ctggaccact cagaaagttt taactaaatg atatttctat catatctctt
    46201 gtggaggagg tgaatctatc catggcaaac aaagcagaat taatcgaaaa agttgcttca
    46261 gctactgatt taactaaaaa agatgcaact gcagcagtag atgctgtatt ttcaactatt
    46321 caagatgctt tagctaacgg cgaaaaagtt caattaatcg gttttggtaa ctttgaagtt
    46381 cgcgaacgcg cagctcgcaa aggtcgtaac ccacaaactg gtgaagaaat cgaaattcca
    46441 gctagcaaag tacctgcatt caaaccaggt aaagcattaa aagacgctgt taaataagac
    46501 agttgaaaaa gcgcttgtgg ctctagggct acaagtgctt ttctttttat ttcctagcag
    46561 aaaaggaata aataagagac agagattccg aatccgtatg tcatacatag aaaacacatg
    46621 tattttattt aatttttatt tatttgcaag ttatgaggtg agctttgctc ttatcttttc
    46681 tagaatttgc ttactaatgg taaaataaaa ataagaagag gagggatgcg tatggaactt
    46741 gattatcaag tagaagatgc gttgacgaaa gtgttttacg gagaaacgct agtcagtcag
    46801 ctaagagaac tttccattga tcagccaatc ttatttttaa cgaatcagcg ctattatgat
    46861 ttgtttgcag ataagatcaa cccgttattt gcaaatcaag caaacaatga ctggtatatt
    46921 tgtgccaaca ctcaatgcaa tcatttaaca gaattaaaga acctgcttga tttcgtcaaa
    46981 cgctatcccg aaaatcaatc catgtttatc atcggatttg gaaatgaagg gatcatggat
    47041 ctggcaggtt tttttcaaaa acatacaacc cttgattcat cactatggat gatccctgta
    47101 tcgatccgtt ctctaagccg agcattaaca ccgcagcgga cgattttgca gctaccaaat
    47161 cgtgtagtgc tgcaaaccat caatctgcca gaacgcatcg tttatgatca aacaatcagc
    47221 gagcggcagg tggacggaaa gcagattgac tttttgactt ttatctgttg cggaatgatt
    47281 tgtgatcatc gttttttgca aaatctgtat aaaaactatc caaataaaaa gcaattaacg
    47341 actcggccgt ttgccgggat gatcgaagaa atgatctatt tttatcaatt agaggcagaa
    47401 aacctgtcaa attatggtaa actgtttgaa caagcttttt atctgaccga aaatggccat
    47461 ctactttctg ctagtatgaa aaaattttta ggaatgatgt ttcatttcgt gtggaatctc
    47521 caagcatatc cttttgattt ccaactaaaa aacttttttg tgtggctgga cttgcttggc
    47581 tatccaatcc actggctgga acagatttca aagatggagt atttggaaaa a
//
LOCUS       DACDYM010000013        47334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDYM010000013 DACDYM010000000
VERSION     DACDYM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.013"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            125..1168
                     /locus_tag="IWM59_000759"
     CDS             125..1168
                     /locus_tag="IWM59_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9978812.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1230..1694)
                     /locus_tag="IWM59_000760"
     CDS             complement(1230..1694)
                     /locus_tag="IWM59_000760"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9978813.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            1940..2887
                     /locus_tag="IWM59_000761"
     CDS             1940..2887
                     /locus_tag="IWM59_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9978814.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(3098..5170)
                     /locus_tag="IWM59_000762"
     CDS             complement(3098..5170)
                     /locus_tag="IWM59_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9978815.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(5103..5480)
                     /locus_tag="IWM59_000763"
     CDS             complement(5103..5480)
                     /locus_tag="IWM59_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9978816.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(5609..6169)
                     /locus_tag="IWM59_000764"
     CDS             complement(5609..6169)
                     /locus_tag="IWM59_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9978817.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            6319..7611
                     /locus_tag="IWM59_000765"
     CDS             6319..7611
                     /locus_tag="IWM59_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9978818.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(7671..8789)
                     /locus_tag="IWM59_000766"
     CDS             complement(7671..8789)
                     /locus_tag="IWM59_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9978819.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="IWM59_000767"
     CDS             complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="IWM59_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9978820.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="IWM59_000768"
     CDS             complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="IWM59_000768"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9978821.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            11995..12594
                     /locus_tag="IWM59_000769"
     CDS             11995..12594
                     /locus_tag="IWM59_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9978822.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="IWM59_000770"
     CDS             complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="IWM59_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9978823.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(14289..14633)
                     /locus_tag="IWM59_000771"
     CDS             complement(14289..14633)
                     /locus_tag="IWM59_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9978824.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(14770..15906)
                     /locus_tag="IWM59_000772"
     CDS             complement(14770..15906)
                     /locus_tag="IWM59_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9978825.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(16095..17069)
                     /locus_tag="IWM59_000773"
     CDS             complement(16095..17069)
                     /locus_tag="IWM59_000773"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9978826.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(17149..18024)
                     /locus_tag="IWM59_000774"
     CDS             complement(17149..18024)
                     /locus_tag="IWM59_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9978827.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            18154..20061
                     /locus_tag="IWM59_000775"
     CDS             18154..20061
                     /locus_tag="IWM59_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9978828.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(20602..23880)
                     /locus_tag="IWM59_000776"
     CDS             complement(20602..23880)
                     /locus_tag="IWM59_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9978829.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(24151..24696)
                     /locus_tag="IWM59_000777"
     CDS             complement(24151..24696)
                     /locus_tag="IWM59_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9978830.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(24708..25400)
                     /locus_tag="IWM59_000778"
     CDS             complement(24708..25400)
                     /locus_tag="IWM59_000778"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9978831.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(25419..25703)
                     /locus_tag="IWM59_000779"
     CDS             complement(25419..25703)
                     /locus_tag="IWM59_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978832.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(25716..26279)
                     /locus_tag="IWM59_000780"
     CDS             complement(25716..26279)
                     /locus_tag="IWM59_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9978833.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            26507..27169
                     /locus_tag="IWM59_000781"
     CDS             26507..27169
                     /locus_tag="IWM59_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9978834.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            27293..28489
                     /locus_tag="IWM59_000782"
     CDS             27293..28489
                     /locus_tag="IWM59_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9978835.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(28590..29966)
                     /locus_tag="IWM59_000783"
     CDS             complement(28590..29966)
                     /locus_tag="IWM59_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9978836.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(30003..30869)
                     /locus_tag="IWM59_000784"
     CDS             complement(30003..30869)
                     /locus_tag="IWM59_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9978837.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(30958..31983)
                     /locus_tag="IWM59_000785"
     CDS             complement(30958..31983)
                     /locus_tag="IWM59_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9978838.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(32001..32675)
                     /locus_tag="IWM59_000786"
     CDS             complement(32001..32675)
                     /locus_tag="IWM59_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9978839.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(32859..33752)
                     /locus_tag="IWM59_000787"
     CDS             complement(32859..33752)
                     /locus_tag="IWM59_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9978840.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(33826..35523)
                     /locus_tag="IWM59_000788"
     CDS             complement(33826..35523)
                     /locus_tag="IWM59_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9978841.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(35516..36829)
                     /locus_tag="IWM59_000789"
     CDS             complement(35516..36829)
                     /locus_tag="IWM59_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9978842.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(36829..40347)
                     /locus_tag="IWM59_000790"
     CDS             complement(36829..40347)
                     /locus_tag="IWM59_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9978843.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(40640..41098)
                     /locus_tag="IWM59_000791"
     CDS             complement(40640..41098)
                     /locus_tag="IWM59_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9978844.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(41396..41914)
                     /locus_tag="IWM59_000792"
     CDS             complement(41396..41914)
                     /locus_tag="IWM59_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9978845.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(41916..42533)
                     /locus_tag="IWM59_000793"
     CDS             complement(41916..42533)
                     /locus_tag="IWM59_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9978846.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(42530..43384)
                     /locus_tag="IWM59_000794"
     CDS             complement(42530..43384)
                     /locus_tag="IWM59_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9978847.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(43381..44292)
                     /locus_tag="IWM59_000795"
     CDS             complement(43381..44292)
                     /locus_tag="IWM59_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9978848.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(44333..45667)
                     /locus_tag="IWM59_000796"
     CDS             complement(44333..45667)
                     /locus_tag="IWM59_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9978849.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            45875..46705
                     /locus_tag="IWM59_000797"
     CDS             45875..46705
                     /locus_tag="IWM59_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP9978850.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(46949..47198)
                     /locus_tag="IWM59_000798"
                     /pseudo
     CDS             complement(46949..47198)
                     /locus_tag="IWM59_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        13596 a        10137 c         8453 g        15148 t
ORIGIN      
        1 tattcgaaac aagaggtcgt acaagacttt tgtcacgacc tcttgttctt ttgtcaaaat
       61 actggtttag tgctactatg ttcgagtatg cttttagtat ttattgacga aaggaaccga
      121 tcaaatgaaa tcattgtcac atccaataaa atctgtaaag gattcagcgg tccgcgaaat
      181 cttacccggt ttactgcttt cttttgccat cgctgccttc agcaggcttc ttgctattta
      241 tctgccacag ttaggtggag cgactattgc tattttattg ggtattttat taggaaatac
      301 tttttttaaa cagcctgttc tagcaatcgg cacaaaattt gctgaaagca aactattaga
      361 attctctgtt tttttactag gtgcaactgt cacagctcaa acgattgctc aaattggtgt
      421 aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact atcatcggtg cttacttgat
      481 tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg atggctggag gaaacgctgt
      541 ttgtggttct tctgctattg cttcaatcgc cccttccatc catgcgaaag aggaagaaaa
      601 aggacagatc atcacactag tcaatctact gggtactgtg atgatgttga ctctcccttt
      661 tcttggatta cagttgtttg gtggaaatct cattgaaaaa agtgcacttt tgggtggtac
      721 actccaatct gttggtcaag tagtcgctgg ggcaagctta cttgacaatt ctgttgtcga
      781 attcgccatg ctatttaaga ttctacgtat tatgatgctc gttgttgttg tctatctgtt
      841 tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa acgccaactg atacgcagaa
      901 aaaacgaaaa ttaccgcttc cttggtatgt attagggttt gtgatctttt gtttggtcaa
      961 cagcttgttt tctttacctg tagttattga ccaatcggca cattttatca gcagctggtt
     1021 tgagatcact gctttagcgg caattggtct gcgtttagac tttgcaaaat tcatgaaaga
     1081 aggtacacgc ttcttatctt atggattagc tgtcgggctt ctccaaacag tgatggcttt
     1141 actatttatt tggctgatca aagtttagaa tgaacatgaa aaaacagttg tgattctcgc
     1201 atggaacaat gtccgaatca caactgtttt taattttgca gatatccttc taacaacaac
     1261 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
     1321 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
     1381 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
     1441 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
     1501 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
     1561 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
     1621 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
     1681 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
     1741 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
     1801 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
     1861 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
     1921 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
     1981 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
     2041 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
     2101 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
     2161 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
     2221 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
     2281 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
     2341 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
     2401 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
     2461 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
     2521 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
     2581 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
     2641 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
     2701 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
     2761 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
     2821 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
     2881 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
     2941 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
     3001 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
     3061 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
     3121 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
     3181 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
     3241 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
     3301 tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
     3361 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
     3421 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
     3481 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
     3541 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
     3601 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
     3661 gcaatccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
     3721 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
     3781 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
     3841 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
     3901 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
     3961 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
     4021 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
     4081 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
     4141 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
     4201 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
     4261 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
     4321 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
     4381 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
     4441 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
     4501 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
     4561 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
     4621 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
     4681 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
     4741 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
     4801 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
     4861 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
     4921 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
     4981 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
     5041 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
     5101 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
     5161 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
     5221 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
     5281 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
     5341 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
     5401 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
     5461 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
     5521 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
     5581 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
     5641 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
     5701 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
     5761 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
     5821 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
     5881 gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct tcctgaccac
     5941 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
     6001 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
     6061 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
     6121 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
     6181 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
     6241 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
     6301 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
     6361 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
     6421 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
     6481 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
     6541 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
     6601 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
     6661 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
     6721 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
     6781 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
     6841 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
     6901 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
     6961 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
     7021 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
     7081 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
     7141 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
     7201 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
     7261 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
     7321 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
     7381 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
     7441 tccagtgcat ttggttatcg tggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
     7501 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
     7561 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
     7621 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
     7681 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
     7741 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
     7801 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
     7861 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
     7921 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
     7981 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
     8041 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
     8101 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
     8161 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
     8221 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
     8281 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
     8341 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
     8401 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
     8461 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
     8521 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
     8581 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
     8641 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgtcaat
     8701 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
     8761 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
     8821 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
     8881 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
     8941 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
     9001 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
     9061 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
     9121 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
     9181 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
     9241 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
     9301 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
     9361 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
     9421 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
     9481 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
     9541 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
     9601 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
     9661 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
     9721 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
     9781 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
     9841 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
     9901 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
     9961 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
    10021 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
    10081 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
    10141 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
    10201 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
    10261 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
    10321 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
    10381 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
    10441 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
    10501 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
    10561 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
    10621 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
    10681 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
    10741 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
    10801 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
    10861 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
    10921 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
    10981 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
    11041 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
    11101 agctgttctt gtgataattg gctaaggaag taagtttgat ctttattatt gtctactcca
    11161 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
    11221 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    11281 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    11341 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    11401 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    11461 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    11521 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    11581 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    11641 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    11701 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    11761 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    11821 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    11881 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    11941 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    12001 tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
    12061 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    12121 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    12181 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    12241 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    12301 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    12361 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    12421 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    12481 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    12541 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    12601 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    12661 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    12721 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    12781 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    12841 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    12901 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
    12961 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    13021 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    13081 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    13141 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    13201 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    13261 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    13321 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    13381 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    13441 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    13501 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    13561 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    13621 ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat aaacaaagac
    13681 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    13741 gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc ccagcgatcc
    13801 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    13861 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    13921 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    13981 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    14041 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaatacctt
    14101 tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa taaatcagca
    14161 aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt
    14221 cgtccttaca aagttagtca tctaacaata ggccgtttca aaactagttg tctttttata
    14281 tccacagatc attcgacttt ttcgaataac ccacggctaa tcatcccatc catcagaaaa
    14341 taaaatttat cttggctcat tttctgtttt ggatctttga aaatattgac tgctttcaat
    14401 ccatttgctg tagtgatcga cattttcaat gtttgaatat cttttgatac agtgattttt
    14461 aatttgaatc cttctttgct ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg
    14521 cctccgtttg tttctgtaaa tccattatcg cgcaatgtat atctttttgc atcaggatgc
    14581 aaacgatagc taattggtgc gcccagtacc gttgctgttg tttcaaatgc catgattttc
    14641 acctcgataa aattagtttc tctttattat aaaagtttat cctgattttt actaggtttt
    14701 tcagttaagt tccctaaaaa atgaaggcgt tctctcagac atctgttcat ataagaatga
    14761 cttcactagt tattttttga cagcacgaac taattgatca aggaaatact ggatgtcgtc
    14821 ttcggtattt cccaaaccaa agctgatacg gatggattct tttagtatcg gtgtatcttc
    14881 tccatacatt gcttctaata cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc
    14941 agtagaaata gcgaccccat taagatcaag cttcatcagt agcaaatcat ttgcttcgcc
    15001 aattaagcga agattcaaca catgagggag tttattcgtt aaatcaccat ttacttgata
    15061 agggatttcg tttgcttcta atgtatcaag aatcaattgc tggaatcctt tatatttgtt
    15121 gttacgtgct tcacgttctt caggtgacag cagttcaact gcttttgcca taccccagat
    15181 ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa
    15241 tgcagggata tttactccgt ctcgtttata taagaaaccg acgccttttg gcccattgat
    15301 tttatgagca gagatactta ataaatcgat tcctaattct tctggatgga tcgtttggtt
    15361 tccgtacgct tgtaccgcat ctgtatggaa ataagctgga tgatccgcta aaagttcacc
    15421 gatttctttg attggcatca aatttccgat ttcattgttc ccgtacatca cagagaccaa
    15481 gatcgtatct tctcgtaacg cttttttgac ctcttcgatc gacagattcc ctttttcatc
    15541 tacaggcaga tatgtaacgt caaacccctt tgtctctaaa tatttcatgg tattaagaac
    15601 agcaggatgt tcgatagctg tagtgattag atgtctgcct tctttttgac gcgagaacgc
    15661 tgtttctaag atagctgtat tatccccttc tgttccccca ctgttaaaga tgatctcgtg
    15721 aggttttgct tgcagactgt ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc
    15781 gtggcgacca aatccatgta tgcttgaagg atttccaaac gtgtcttgca tgattgccgt
    15841 catcgcttca attacttgtg gatgcatggg ggtagttgcc gcatgatcca gataaatagt
    15901 tttcacttca ttcatccctt ccaaattatt gctaggcaca ttctagcata aaatagcgaa
    15961 caaaccaaag aagagctcat ttttgtctag aggacgtttc atgggcatag aaaaaaggag
    16021 ctgtgacaaa agtcttgtcg cagctccttg aagctgctca cttctgtcac aacttctttc
    16081 cctctttttt ttgtttattc gaattctttt tcttccagac ggaataacgg actcatcggt
    16141 gtattttcat ggatgcgttt gaccgcttca cccattaatt ctgaacatgt aacgattgtt
    16201 aagttttctg gatgacgatt ttcttctgtg aaaacagaat cagtgataca aatatctttg
    16261 attggtgcat tgtctaaaat acttttcgca tgatcggaca ataatccatg tgacgcacag
    16321 gcatatactt cttttgcgcc gttttctttt aatacatttg ctgctcgtgc aagcgttgct
    16381 cctgtgttca agatatcatc taccatgata catgtctttc cttgaacatc accgatcaca
    16441 taaccaccct cagttgaagc atcatctgca tgatctacga tggctaatgt gctgttcaaa
    16501 tattctgaaa gactacgtgc acgctgaaca ccgctgtttt ttggcgaaac aactacgatg
    16561 tcatctcccg tcagacctaa ttgacggtaa taatgagcaa acaacggcat cgtaaataaa
    16621 ttatctacag gaatatcaaa gaatccttgt acttgaaccg tgtgcagatc taaagtcagt
    16681 actcgagttg cccctgcttc ttcgatcaag ttagcgatca atttcgcagt gatcggttca
    16741 tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt aaggcaatac gacattgatc
    16801 gttttcgcac ttgctcgctt cattgcatcg atcatgatca gtagttccat atagtaatca
    16861 ttgacaggtg cattcgttga ttgaacgata tacacatgat cccctcgaat actttcttcg
    16921 atattgatgg aaatctcgcc atcactaaat tgcttcacga cgcttttacc taattctgtt
    16981 ccgaacactt tcgcgatttt ttcagctaat ggacggttgc cattcaaact aaaaatcttc
    17041 aacgtgtcat cctgatactt attactcatg tagtcctccc caaaacttct ctattcttcg
    17101 atagtttaac atatttttcc aagattttct gttaatttca gcaaattctt aaggaagtga
    17161 aactgttccg ttactccaac tggcaggaat gactttatga taatcgcccc agttgtcgtg
    17221 tactttgtaa taacgttctc ctgtttcagt ttcaaaataa gcgtacgctg tcacccaatg
    17281 attgccatat gtactgccaa gtactttcaa aataccaatc atcacaggtt tgccttcacg
    17341 tatacgtttt gtagctcgct gccatgaacc tagaagagtt gctcgtgcac gctcatgatt
    17401 gccattcttc tttaaaaaac tagaaatccc tgtactaacc tgaaaaggaa tcgtaggaag
    17461 tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa accaacgaat cagccgtatt
    17521 tttcttgcga atctcatttg gaagcatcca ccccttacga tagtcttgat aataggcaag
    17581 gagaactgct gccgcgtaag taccacaaag atagcctgag ggggtagccc atttcttata
    17641 cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt gcgagttcct ttcctgacct
    17701 tttttcttcg atcaaaacgg gaatatcttc ttttctgttg atccatctag ataactcgat
    17761 tggctcttca ttgatcagct tccatttttt acaagaaaga ccctcttgat aagtatattt
    17821 tgctggagaa tattctaccg gatgtcctga ttgatcgata atcacataac tttgatcttc
    17881 aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac tgcagctgct gagtgctggc
    17941 taatttttta atttttttat ctactttgaa atataaaggt ttatccacta cttgattcag
    18001 ttgggatcgc ctcctttttt tcattataag tgaaggattg aaaaaaacaa atcagtttga
    18061 ttgttcgaat tttcaatttt ttatgttcta tttgttaatt ccttcatttt gtaagataat
    18121 gggcacacga aacaaaaaag gagcgatcga tgaatgaaca aagaacgatt gaaacaagat
    18181 ttctatgaag cagtcaatga agaatggcaa aaaacagcaa aaatccctgc agataaacca
    18241 gctacaggcg gtttccaaga tttagttgac gggatcgacc aacttttgat gggagaaaca
    18301 gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca
    18361 tattatcaac tggcaaacga ttatgaccga agaaatgagt tgcatgctca accgcttcaa
    18421 cctataatcg aaaaagttga atcattgcag tctttcgacg aattgaataa gcaatttcca
    18481 gaatggctgt tggatacatt accattgcca tttaatctgg atgtagatgc agatatgaag
    18541 aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt tattccttcc agataagacg
    18601 tattatgcat cagaccatcc caacggtgca caactaatag gcgttttctt tgatatggct
    18661 gttcagttat tagagcttgc cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa
    18721 gcagtgcaat ttgataaatt gattgctcct catgtaaaaa gtgcagaaga aaacgcagat
    18781 tacagcaaaa tgtataatcc gcaatctttt gatacattta ttaaatacac agataaactg
    18841 gacttgaaaa aacttgtcac gggtttgatt gggacaactc cagataaagt gatcgtaact
    18901 gatccagtgt acttcgatca tttgaatgac atcctgacag aagctcattt tccgttattc
    18961 aaaagctgga tgatcattaa aacagtacgt tctcttagtg gttatctatc agaagaattc
    19021 cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag
    19081 aaaaaagcag cgtattacct tgcctctgga caattcgatc aagtagttgg cgattactac
    19141 ggaaaaaaat attttggcga agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg
    19201 atcgccgttt ataaaaaacg tctagaagaa aatgactggc ttggggatgc aacaaaagaa
    19261 aaagccgtca taaaattaaa taacttaggt atccaagtag gctaccccga taagatccca
    19321 gcattattcg accagttcaa aacagtacct gcagatcagg gtggtacttt gctttcaaat
    19381 actcttgttt tcagcaaact cacattaaaa gatcgtttca gcaaatggaa caaaccagtg
    19441 gatcgttcag aatgggaaat gagtgctgac acggtcaatg cttattatca tccatttcgg
    19501 aatgtgatcg tctttccggc agcgatcctt caagcaccat tttacagtct agaacaatca
    19561 agctctgcta attatggtgg aatcggagct gttatcgctc atgagatttc ccatgcattt
    19621 gataataacg gcgctttatt tgacgaatat ggcaacttga acaactggtg gacagaagaa
    19681 gatcttacac aatttcaaaa caaagcacaa gcaatgatcg aagaattcga tggtctgcct
    19741 tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg aaaatattgc tgatgcaggc
    19801 ggtctaagct gtgcattgga agcagcaaaa acggaatcag atctttctat cgaggaattc
    19861 tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa aagaatacca acaactttta
    19921 ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt
    19981 gacgaattct atgaaacatt tggtatcacg gaacaagacg ctatgtatct agcgccagaa
    20041 aaaagagtgc gaatctggta agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa
    20101 ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt cggcttattt ttcgaagaag
    20161 ttacttctgg aataccatgt attagaggtt gtgaaaaagc tgcttagttt caagtagtaa
    20221 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta ctaccgagaa
    20281 actggctttt gaacaccgtt tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt
    20341 agagaaaaaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
    20401 gtcagctgat tgaacaccgt ttagtggaga gattgtgaaa aagctgtcct gcatcaagta
    20461 ataagaacag ccaatcaaaa gcagcaagga gaacctcatc tataaaaaga tagaaggaag
    20521 tgagacatct attggtctca tttccttcta tttttacttt cctcagtcaa ttgttttaca
    20581 tcataaaagg agaaattcca cttatttttt tcgctgtttc atgacataaa caactacacc
    20641 gattaacaaa attcctccag ctaatgctag caacgggtta ttaaattctc ccgtttttgg
    20701 gagtccttga gtgccatttt tttcactttc ttttgctgtc ctatccggat gagaaggttt
    20761 tccatttggt tttccatttg gtgattcatt tcctggattt tctctttctg gtttgtcatt
    20821 tcccgggggc acatcggcag tttctcgcac agtgatcgct ttttgctggg taacagattc
    20881 tgtatgagca gaccattttt ccccatcaaa tatttccgaa cgataagtga cttctagcgt
    20941 atattctcct tttgaaggtg ctgaaaaaat agcggtatga ttagaaaatt gagcacttga
    21001 ctgtgtttca acgatttgga tcttttctgg gaaataacgc atatcttcaa agacaggcgt
    21061 tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt
    21121 tccttctgca ttctccgcca caccatttgc ttcttgtata ataatcggat tattttgttc
    21181 atttggtttt gtctgatttg gttcttgtac gattgcatac tctggatatt ccgaagacgg
    21241 tgaggtttct ccgtcaaaaa tagcacctac agataatgga agaccatcca ctctttcctt
    21301 ttctgtttga cgattccccg gtttaatctg catcagtcca tgaacatgga tacttccatt
    21361 ttcatagcgc gtaacaggct gctgaccttc tttcgaacat gtatttagag actcgatcca
    21421 atcttttgat atggtatgtc caagagtatt ttctgattgt ttctctcgtt cattccaata
    21481 gtagttcaac gagtggtaga tttgatcttc gttttgattc aatgggcgga tctgctctct
    21541 catctcaaga ttctctgttc ggaaagataa gataccagaa gcatggtagt ctgtgtttcc
    21601 tgctgaagag gtataaagag ccacattata tgaaccgtta ttaaaacttg tactgctatt
    21661 gacgataata tctgttccgc tattggaatc gattcctttc gcaagatttt cgaaagacaa
    21721 actattgatc aattcatgag ggcccgttaa agaagaaccg cccattttga atccattacc
    21781 gttaccttct ccctcaattc ctggtaccca gccgtttcga taagcaactg agtttttgat
    21841 gattactttt ccgatcgaac ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc
    21901 attatgatac gcgatacaac cgtcaaatac atttccttct cctattgtca gtttggccgc
    21961 aaatccatcg gcatcttcaa atcctggatc ggcattttca aaagatgtac agttcttgac
    22021 taagttatgc gcgggccatg cttcgaaagg atcatttgag ctaccgctga tttggattcc
    22081 tgtatttcca ttccgataag catgaatctc ttcgatcaaa ttatgatttc cagaaatctg
    22141 taatcctttt tgcatagctg cactatttgt aatgtcaaac cctcgtaaac cccagtagtg
    22201 actgaacagt gtcatcccat ttccagttcc tttaaaatca agtacaggac gcttgttttc
    22261 tggatttttc tctgcaatca actgaatcgg gttttctctt gtcccgttaa cgccttttgg
    22321 aatcgtcaat cctctttcga aggaataagt tccaggcgcc atgacgattg tctgcccagc
    22381 atatgcgtat ctcaatgctt gtgtcagaga taatggattt tgtctgcttc cgtttcctgc
    22441 atctgaccct tctggtgtta cataaactgt cgaatattta ggtttacggt agtccactgt
    22501 atgaagaatc tcttttgttt cataactagt catctctacg tattcttctg gcggatagtt
    22561 tttatctggc gtaaaactga aagtaaattc gttcgttcct tccatcagtt ttgtctgata
    22621 atcaaattcc cgattcgcag tgatagccaa gttttttgcg ttcttaagca cctctcctgt
    22681 tgctttattt ttaattgtca ataatccgtc agcatttgct ctaaaggaca tagtatgatc
    22741 ttggtttcct gaatgattac tagaggtcac agtgtaaatc ggatcaatca agcgagtagg
    22801 tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa gcaacttcga ttccttctac
    22861 cttgatcgtc atattccttg ctgcagcaaa tcctgcataa ataaccgatt catctgaagc
    22921 gtataatttt tcccagtcat acatgatgtc agaagccatt tcttctcctg tttcaggatc
    22981 gtaataatat gcctctaaac cagtattcgt ttttttcatc tctacgtcca gtttagtaat
    23041 cgagttcgtg gccggaaaca gattcccatg accgtttccg aatatattat aagaacctgt
    23101 ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt gtttccactg agacactccc
    23161 aggagctggc gcagcttgcg gatcttcaga tgatattcct gtgattgccc gtgtccccaa
    23221 tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc acttcttgtc tatccgagtc
    23281 ccagacatat tcgatccgac tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc
    23341 aaacaaatga ttttttggta ctgcgtctct taccattaaa gcaaatcctt cttgggcatt
    23401 cgtataggtc caagattcta tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg
    23461 gattgcttga taataatagg tcattccatc tgatccgctt gactggaatt ttccgccgtt
    23521 attcgtggaa gagagccgca cgccgtcttc taatggttca atggcatacc tactagcact
    23581 tgtgctttct ccgaaaacaa cccctttcca ctcttcattt gcttcttcag attgcttttt
    23641 tgtcttttca ttttctgatt ctttcatcat aggatcattt ccttcttccg tatccatatc
    23701 ttcactagtt cttggtattt cctccattgt tgacatccct tcaatgttca cgtgtgcatt
    23761 ttcttcgttc acagactgtt gttcgttagc agcaacttca acgacagaaa ttaaaaccgg
    23821 ggtactgagc ataaggagat tcatcattag cgcactgtat tttatacgtt tttttaacaa
    23881 gtgtattcct cccgtaacaa attagaaagc gtttacttaa agtattacct aatttatttt
    23941 tactgtcaat aaaaaacaaa attataataa gtgtatttta taaaaagaaa aaatataact
    24001 taagttgttg ataataaaga gatttattaa cgtaaaaaga ataagttttt ttaaaataac
    24061 gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta ttattttgtg
    24121 ctttttattt tatcaaacca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
    24181 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg catctttgaa
    24241 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
    24301 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
    24361 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
    24421 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
    24481 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
    24541 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
    24601 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
    24661 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
    24721 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
    24781 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
    24841 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
    24901 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
    24961 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
    25021 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
    25081 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
    25141 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
    25201 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
    25261 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
    25321 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
    25381 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
    25441 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
    25501 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
    25561 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
    25621 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
    25681 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
    25741 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
    25801 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
    25861 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
    25921 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
    25981 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
    26041 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
    26101 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
    26161 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
    26221 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
    26281 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
    26341 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
    26401 tctttagtcc gatactcttt atagtagtcc gtatgctaaa ataagtatgt aatcgaataa
    26461 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
    26521 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
    26581 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
    26641 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
    26701 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
    26761 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
    26821 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
    26881 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
    26941 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
    27001 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
    27061 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
    27121 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
    27181 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
    27241 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
    27301 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
    27361 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
    27421 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
    27481 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
    27541 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
    27601 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
    27661 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
    27721 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
    27781 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
    27841 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
    27901 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
    27961 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
    28021 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
    28081 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
    28141 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
    28201 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
    28261 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
    28321 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
    28381 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
    28441 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
    28501 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
    28561 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
    28621 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
    28681 acagtttgca gacgaagcat caatacaata caagtcaaga tggagacgat tggcaatagc
    28741 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
    28801 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
    28861 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
    28921 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
    28981 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
    29041 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
    29101 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
    29161 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
    29221 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
    29281 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
    29341 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
    29401 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
    29461 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
    29521 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
    29581 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
    29641 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
    29701 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
    29761 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
    29821 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
    29881 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
    29941 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
    30001 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
    30061 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
    30121 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
    30181 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
    30241 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
    30301 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
    30361 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
    30421 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
    30481 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
    30541 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
    30601 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
    30661 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
    30721 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
    30781 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
    30841 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
    30901 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
    30961 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc
    31021 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
    31081 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
    31141 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
    31201 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
    31261 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
    31321 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
    31381 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
    31441 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
    31501 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
    31561 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
    31621 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
    31681 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
    31741 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
    31801 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
    31861 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
    31921 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
    31981 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
    32041 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
    32101 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
    32161 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
    32221 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
    32281 tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg
    32341 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
    32401 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
    32461 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
    32521 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
    32581 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
    32641 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
    32701 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
    32761 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
    32821 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
    32881 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
    32941 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
    33001 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
    33061 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
    33121 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
    33181 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
    33241 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
    33301 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
    33361 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
    33421 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
    33481 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
    33541 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
    33601 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
    33661 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
    33721 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
    33781 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
    33841 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
    33901 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
    33961 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
    34021 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
    34081 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
    34141 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
    34201 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
    34261 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
    34321 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
    34381 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
    34441 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
    34501 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
    34561 aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
    34621 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
    34681 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
    34741 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
    34801 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
    34861 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
    34921 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
    34981 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
    35041 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
    35101 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
    35161 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
    35221 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
    35281 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
    35341 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
    35401 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
    35461 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
    35521 catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc
    35581 ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg
    35641 attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg
    35701 tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt
    35761 ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca
    35821 cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt
    35881 ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg
    35941 taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat
    36001 tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata tctaaataat
    36061 atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt
    36121 tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga
    36181 catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc gggtgataaa
    36241 ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt
    36301 ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag
    36361 gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta
    36421 ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata ttgtttctgt
    36481 tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt
    36541 catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct
    36601 gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat
    36661 ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt
    36721 ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg actgctgcaa
    36781 gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc
    36841 ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa
    36901 tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc
    36961 aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc
    37021 taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac
    37081 ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc
    37141 tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt
    37201 cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc
    37261 gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt
    37321 ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag
    37381 ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat
    37441 ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa
    37501 cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg
    37561 tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt
    37621 taaatcgaca tacttttcta cacctacggc acgagaatag aagtatccga tcccaggcaa
    37681 acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga
    37741 agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc
    37801 taattttcta actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca
    37861 taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg
    37921 cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc
    37981 atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt
    38041 ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac
    38101 tttctcacca tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct
    38161 ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat
    38221 ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt
    38281 actgccttca attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc
    38341 cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga
    38401 aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac
    38461 tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt
    38521 ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat aacgtggtgg
    38581 tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat ctcctaaaat
    38641 cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg
    38701 ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg
    38761 ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat
    38821 ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt
    38881 ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc ccgcatcaag
    38941 ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt
    39001 ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc
    39061 ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc
    39121 tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt
    39181 aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat
    39241 atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac
    39301 gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg
    39361 ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt
    39421 ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt
    39481 ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg
    39541 agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc
    39601 ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt
    39661 attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc
    39721 ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat
    39781 cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt
    39841 tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt
    39901 aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg
    39961 attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc
    40021 tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag
    40081 cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt
    40141 ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat
    40201 tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt
    40261 tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt
    40321 tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt catttcccca
    40381 aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt
    40441 agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat
    40501 caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt
    40561 ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcatctatt ctttcatcaa
    40621 ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa
    40681 tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat
    40741 cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac
    40801 gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac
    40861 cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt
    40921 caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg
    40981 tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg
    41041 gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat
    41101 ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca
    41161 tctaattata atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa
    41221 aacaccaatt atacaacatt tattattaaa attaaattat aaaattttat aacgttctca
    41281 tttaacaaaa cttaagttat taagacaaat gaataaacaa ctacgctttc ctcgaaacaa
    41341 aaaaataaag cacaaatgcg catttacgca ctcatgcttt attcgtacaa ttaattcaga
    41401 taacggcagc gcataggatg ccaataacac ttcctaaaat agctcctcct aaaatgtcag
    41461 taggataatg taaaagtaaa aaaatcctcg aataagcgat caaacaagca aataaaaagg
    41521 ctaatattcc tgcaactgga cttaattcga acaacgcagc tactgataaa aaggctagcg
    41581 ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg acaagccgac
    41641 gatttttttc ataaggtctt ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa
    41701 aaaaatagat caaactgccg ataagaatgg ctagaccata ttctctagta tcaggtatca
    41761 agactaaaac agcagcaagg agcaaccaaa taaaaccacc attagagata ttactgataa
    41821 atttcaaaaa attgattttt cgactatccg ttttccgttg cttggcgaac ttgttgataa
    41881 tactatcatc gattttagca atcaaacgca acatgttatt cctccttttt aagaaccttt
    41941 tcttgcgtac gatggataaa aagcaggact tgaaaataaa acaagaaact gaaacctaat
    42001 aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc
    42061 aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa
    42121 gcttcttttt gttccagttg aagcaagatt ttgtaactaa agagaagcgc gcttaataga
    42181 agcaaaaaca acgtagtata catcaatgta tagccaattc ccccacccca atagcggaaa
    42241 atctgataat cgatcaatag aagaagaaaa taaacacttg ctaaactatc ttgctttcca
    42301 taaagagaga agttctcacg aaactttttc cagaatagtg aaccgagctt tttctcttca
    42361 ttttttttgt aatgataaat catatacaga actgttcgac ttgcagttcc taatgtgaaa
    42421 acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg aatccattca
    42481 atgatttcta agaaataatc attggctttt ttaccaatcg atcgattcat catccttttg
    42541 aacctcctgt caaatccatc ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa
    42601 tcgtcgggat caagacaagt gttgctcctg ccatcagata attccattgt gtcgttgctt
    42661 ggttctgaaa aacttgcaag ccaatctgta acgtgtattt ttcttgatct tggatatata
    42721 acagaggacc taagaaatca ttccaagctg cgttgaagga attgatcgca atggtaatca
    42781 aggcaggttt cgtcaatgga agcatcaaat gactccaaat atacaaatga ttcgctccgt
    42841 cgatttcagc agcttcgatc aattcattat tgatcgataa gtaaaattga cgcatcagaa
    42901 atatattgaa cgcattgccg aagaacgatg gaacgatcag gggcaaataa gtacctaccc
    42961 agccgatctt ggagaataat acatattgag ggatcatcgt tacaaacccc gggatcatca
    43021 tcgtagaaag aaccaaagca aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc
    43081 cataagcaat aaaagaattt gaaaggacat taccgatcac tactagcaca gtgatgaata
    43141 atgtatttct tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga tagttttccc
    43201 atacgatttt ttctgggaaa aaggtcggag gataacgaac gatttcttcc attggtttca
    43261 atgaagtaaa caccatccaa agcaatggaa tgatcaaaat gattcctatt actgtcatga
    43321 ggacataact tagtattttt tttgtttgcc gattcacact ggtctttttg aaataatcca
    43381 tcatttgccg tcacctccag aataatagac ccacttaggt gcaagcttca aattgacaaa
    43441 cgtcagaacc aacacgatca gaaacaggat cattgacata gccatcgcat aacccatatc
    43501 aaaagctacg aacgccttgt tccacatatg cagattataa aaaagcaaag aatttgcagg
    43561 tccgcctgtt ccatttttcg tcatgacata agcttcttga aaaatttgga aagaaccgat
    43621 cgtagaggtg atcacatcgt aaaaaataat aggagtgatt aatggcaaag taatactttt
    43681 gaactttccc caaacgcctg ctccatcgat ttctgctgat tcatataaag atctaggaac
    43741 attttgtaat gtagctaagt aaagtagcat tgctcctcca gaactccata ttttcatcaa
    43801 aatcaacgca ggtttcgtcc attgtggatc aaacagccaa gctggtccag ggatatggaa
    43861 ccagcccaga aatgtgttga tcaatcctgc agatggagaa agcagctgca tccataagat
    43921 gtagactgct actcctgata agactgccgg taaatagaaa gctgtacgga atacactgat
    43981 tcctttgatt ttttgattga gtactgtggc taaaaacaca ctgaaaatcg tcgtcagcgg
    44041 tacattgaag atgacgaaat aaacggtgtt gtataacgat ttccaaaata aatcatcgtg
    44101 aacgaacatt cttttgaaat ttgctagacc aatgaaattc atcttggatg tcaaattata
    44161 atcggtgaaa ctaccaataa ctgaaaaaag cataggccct agcataaata ccaagaaccc
    44221 gataataaaa ggcgagataa ataaataggc ccatcttgct tcttttttct ttcggataga
    44281 taaacgcttc aaattttctt gcctcctctg tacctagaca gtcaaaaatg gattattgat
    44341 tttgcttgac taagttttct actgcttttt gtgcatctgc caatccttct tttggtgatt
    44401 ttgttcctaa aagaatctgg tcaatctgtt cattcactaa tgtcgtgtaa tccggagcat
    44461 ctaaaggaac tggtgtgatc accgtatttt taaagttttc gtccgccatt tgatagatca
    44521 tttttccttt atccgttagg ttgtcattat tcactaaatt ctcattggct gtcttgctcg
    44581 ccatgatatc aaaacttttt tcgccgaatt tttcttgtac ttctggagaa gaaagatatt
    44641 tgatgaattc ataagaggcc tcaggatctt ttgctccgta tggtacttcc aagacgaatc
    44701 cgccacccca cgaccaatgg cctgaacctt cttctttttc tgggagctgc atcacgccaa
    44761 aatcaaaatc atccggtgca ttttccatca aactagagta ataattgata ttttgggcac
    44821 gcatggctac taatccggaa ataaatggat cagatacacc agacccaaat tcggcttcta
    44881 atttattgat cgtatttttc ccataataat cttgccagtc ttggatccac tccaaggctt
    44941 ccactttatt tttagtatca atcttgactt tttcatcttt gtcaaaccaa ctagtcccgt
    45001 tatctgcgtt tagcgcccat acatcagccc ctaaattcca taatggataa aaaccgatgc
    45061 gtgtaaattc ttttccttct tttttatcta gtttacgtgc tgtttcttct aattcatccc
    45121 aagttgtcgg tagatcttgt tcactgattc cagcttcttt aaataacttt ttattataaa
    45181 aaatgacttg tgtatctgta ttgaatggga tcgcataagg atcaccttca tattctactg
    45241 tatcccaaag ctgcggataa aaatccttag aagtgtcttt ttctacatat ttggagagat
    45301 tagttgcttg ctgtgcttct gctctttggg caacggaatt tatgtcctgt acaataacat
    45361 cgggtgcatt tccagctgtt attgcagata aagatttagt ccagatgtca ccccatggct
    45421 gatacttata atttacatgg atctcgtttt gtgattgatt gaaatcctca atgatttctt
    45481 cgatagcttc ttttcgtgca ccggatcccc aaaaggacca gaaatcaata ttttttcttc
    45541 catcacttgt tttttcagaa gaatttgctg cacttgaact ggcatcgctt gcattacttg
    45601 atccgcatcc tcctaataaa aacaatgctg aacttgccag caaccctgta aacaataatt
    45661 ttttcatatt ttacctccaa attttaatgt aaccgatgcc ggttttaata taacgatatc
    45721 attttgttct gaaaataaca atttataagg tatgattcta aaaaaattat taatgatacc
    45781 aatactttat ataattcgcg agcgctttct ttgaattttt taatctttcg tagcagaatg
    45841 agaatgtatt aaaaatagat taggaggaat tttgatgttt agacaccaga aagaattgca
    45901 gtttgaagca aaaccagatc gtccgaatcc attaatcgcg aaatatctgc aggagctgat
    45961 cggcggccaa tatggcgaga tgagtgtagc tatgcagtac ctgttccaag gctggagctg
    46021 ccgaggagaa gaaaaatata aagatatgct gatggacatt gcaacagaag aaatcggcca
    46081 cgtggaaatg ttggcgacaa tggttgctcg tttattagag ggtgcgcctg ttgaagatca
    46141 agaagcagcg atgcaaaaag atccaacaat cggtgccatt atctctggaa tgaatccaca
    46201 gcacgcgatc gtagctggct taggacctcg tcctgctgac agcgtgggca atccatggag
    46261 cggtgcctat atcattgcga gtggcaactt gttagcagac tttagagcta atttaaatgc
    46321 cgaatcacaa ggaagacttc aagtagcacg tatttacgaa atgatcgatg atcgtggcgt
    46381 aaaagatatg ctgtctgttc tccttgcaag agatagctat catcaaaatc tatgggaatg
    46441 tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta gtcccaagta ctttcccgcg
    46501 tgacaaagaa cgaaaagaca ttgcttatga cttctataac ttctcagagg gagaccaaag
    46561 tagtgaaggt tcttgggcta gtggcaaagc actggatggc gaaggcgaat atcaatggtt
    46621 gaaagaacct aagatcgaag gcgctgcacc aaaattagaa ccagtaacac caaaaatgta
    46681 tgggacacct ccattcaaga aataaatatc tacccgtaga aacacccgtt ttttgttcca
    46741 cacgctttgt gagcaaaact gactaaagtc attaatcata accacccttc aaaagggtgg
    46801 ttttctctag agctataagc tcaaatcacc ggccagcgcc taaagacgct ggctttcata
    46861 ttttatgttc aagccatatt gcctttgcca gttttgccca cctcttaaaa ggtttatgta
    46921 ctactcctta gcccttaaaa ggatcgtctt actctcgtac actcagctta tctaacgcac
    46981 tatcatgttt ttcttgttct tgaatgtact ttttgatggt cgcttcattt tgtcctactt
    47041 ttgtcacata gtatccttcc acccaaaagt gtcgataccc aaatttatat tttagatttg
    47101 cacaatctct tgatgatctc ctgaatattg gctcgatatt gattataaat tatttttctt
    47161 ctatactttg gtgtgaatac aatgtggtat ttacacatct actttgtacg ggctaaacta
    47221 tttcccttta ttgctccacc cgcatagcaa gtggttttgt atttcaatcg ctcggcgcgc
    47281 tcaattaggt ctaaagacaa taaatctaaa aacccgctgc ttcactgaca gaaa
//
LOCUS       DACDYM010000014        45748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDYM010000014 DACDYM010000000
VERSION     DACDYM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.014"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            101..544
                     /locus_tag="IWM59_000799"
     CDS             101..544
                     /locus_tag="IWM59_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9978851.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="IWM59_000800"
     CDS             complement(550..1734)
                     /locus_tag="IWM59_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9978852.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="IWM59_000801"
     CDS             2002..2700
                     /locus_tag="IWM59_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9978853.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="IWM59_000802"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="IWM59_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9978854.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="IWM59_000803"
     CDS             complement(3510..4043)
                     /locus_tag="IWM59_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978855.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="IWM59_000804"
     CDS             complement(4340..6721)
                     /locus_tag="IWM59_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9978856.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IWM59_000805"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IWM59_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9978857.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="IWM59_000806"
     CDS             7422..7961
                     /locus_tag="IWM59_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9978858.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="IWM59_000807"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="IWM59_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9978859.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="IWM59_000808"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWM59_000808"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="IWM59_000809"
     CDS             9068..10234
                     /locus_tag="IWM59_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9978860.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="IWM59_000810"
     CDS             10280..11776
                     /locus_tag="IWM59_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9978861.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="IWM59_000811"
     CDS             11857..12540
                     /locus_tag="IWM59_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9978862.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13185..14375
                     /locus_tag="IWM59_000812"
     CDS             13185..14375
                     /locus_tag="IWM59_000812"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9978863.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14477..15430
                     /locus_tag="IWM59_000813"
     CDS             14477..15430
                     /locus_tag="IWM59_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9978864.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15499..16848
                     /locus_tag="IWM59_000814"
     CDS             15499..16848
                     /locus_tag="IWM59_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9978865.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17031..17354)
                     /locus_tag="IWM59_000815"
     CDS             complement(17031..17354)
                     /locus_tag="IWM59_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978866.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17483..18646
                     /locus_tag="IWM59_000816"
     CDS             17483..18646
                     /locus_tag="IWM59_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978867.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18716..20065
                     /locus_tag="IWM59_000817"
     CDS             18716..20065
                     /locus_tag="IWM59_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9978868.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20110..20541)
                     /locus_tag="IWM59_000818"
     CDS             complement(20110..20541)
                     /locus_tag="IWM59_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9978869.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20929..23571
                     /gene="alaS"
                     /locus_tag="IWM59_000819"
     CDS             20929..23571
                     /gene="alaS"
                     /locus_tag="IWM59_000819"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9978870.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23771..24367
                     /locus_tag="IWM59_000820"
     CDS             23771..24367
                     /locus_tag="IWM59_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9978871.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24489..25637
                     /locus_tag="IWM59_000821"
     CDS             24489..25637
                     /locus_tag="IWM59_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9978872.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25675..26088)
                     /locus_tag="IWM59_000822"
     CDS             complement(25675..26088)
                     /locus_tag="IWM59_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9978873.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26167..26598
                     /locus_tag="IWM59_000823"
     CDS             26167..26598
                     /locus_tag="IWM59_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9978874.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26697..26930)
                     /locus_tag="IWM59_000824"
     CDS             complement(26697..26930)
                     /locus_tag="IWM59_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9978875.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27113..28783
                     /locus_tag="IWM59_000825"
     CDS             27113..28783
                     /locus_tag="IWM59_000825"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9978876.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28930..29316
                     /locus_tag="IWM59_000826"
     CDS             28930..29316
                     /locus_tag="IWM59_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978877.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29303..30001
                     /locus_tag="IWM59_000827"
     CDS             29303..30001
                     /locus_tag="IWM59_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9978878.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30006..30470
                     /locus_tag="IWM59_000828"
     CDS             30006..30470
                     /locus_tag="IWM59_000828"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9978879.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30467..31378
                     /locus_tag="IWM59_000829"
     CDS             30467..31378
                     /locus_tag="IWM59_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9978880.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31613..32152
                     /gene="pyrR"
                     /locus_tag="IWM59_000830"
     CDS             31613..32152
                     /gene="pyrR"
                     /locus_tag="IWM59_000830"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9978881.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32166..33449
                     /locus_tag="IWM59_000831"
     CDS             32166..33449
                     /locus_tag="IWM59_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9978882.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33462..34388
                     /locus_tag="IWM59_000832"
     CDS             33462..34388
                     /locus_tag="IWM59_000832"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9978883.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34407..35687
                     /locus_tag="IWM59_000833"
     CDS             34407..35687
                     /locus_tag="IWM59_000833"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9978884.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35692..36768
                     /gene="carA"
                     /locus_tag="IWM59_000834"
     CDS             35692..36768
                     /gene="carA"
                     /locus_tag="IWM59_000834"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9978885.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36773..39958
                     /gene="carB"
                     /locus_tag="IWM59_000835"
     CDS             36773..39958
                     /gene="carB"
                     /locus_tag="IWM59_000835"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9978886.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40012..40788
                     /locus_tag="IWM59_000836"
     CDS             40012..40788
                     /locus_tag="IWM59_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9978887.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40785..41717
                     /locus_tag="IWM59_000837"
     CDS             40785..41717
                     /locus_tag="IWM59_000837"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9978888.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41714..42430
                     /gene="pyrF"
                     /locus_tag="IWM59_000838"
     CDS             41714..42430
                     /gene="pyrF"
                     /locus_tag="IWM59_000838"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9978889.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42437..43066
                     /locus_tag="IWM59_000839"
     CDS             42437..43066
                     /locus_tag="IWM59_000839"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9978890.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43054..43368
                     /locus_tag="IWM59_000840"
     CDS             43054..43368
                     /locus_tag="IWM59_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978891.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43406..44047
                     /locus_tag="IWM59_000841"
     CDS             43406..44047
                     /locus_tag="IWM59_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9978892.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44078..44782
                     /locus_tag="IWM59_000842"
     CDS             44078..44782
                     /locus_tag="IWM59_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9978893.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            44779..45681
                     /locus_tag="IWM59_000843"
     CDS             44779..45681
                     /locus_tag="IWM59_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9978894.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
BASE COUNT        15005 a         7997 c         9883 g        12863 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt tgtcacagtc
    12781 cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa aattaaaaat
    12841 tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa taaataaatt
    12901 aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt gtgttgtatt
    12961 tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc atactatata
    13021 agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa ttctcataat
    13081 cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga cacgatatat
    13141 gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct tccaaagaac
    13201 taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta tacaatacga
    13261 tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa aagatgatca
    13321 atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa aaagcatgta
    13381 ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc aatggttcgc
    13441 tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca gaagcagcaa
    13501 tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta atgacaaaag
    13561 aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt tgccgcttat
    13621 ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag aaaaaagcaa
    13681 aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat ggtgttttct
    13741 ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa aaatatggac
    13801 tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt gcaggaaccg
    13861 tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta tcgaaagcta
    13921 taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg ttgaaagcta
    13981 gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca gctgcattgg
    14041 cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg gaaaatgcta
    14101 attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct gaaacaccta
    14161 ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag aagctgatcg
    14221 aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg acaggcagga
    14281 tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca gtagcagttt
    14341 atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt tcgtctattt
    14401 aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt agtaaaaaag
    14461 atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac aaattggttc
    14521 agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg cgacagatat
    14581 acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg taatggatta
    14641 cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc atttggctgc
    14701 tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga acgtgactgg
    14761 gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta cgccaagttc
    14821 aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata ctcttcagcg
    14881 tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg attattatta
    14941 tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt cttatgcaac
    15001 cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag caatcaaaga
    15061 aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt atatgccaga
    15121 tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg ttcatcgaaa
    15181 tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat ctattcaaaa
    15241 aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt caatagcaga
    15301 atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct tttcgccgaa
    15361 atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa aattggtaga
    15421 gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga ttggctatgg
    15481 tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga agtaaaaata
    15541 gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt tatgggcaca
    15601 gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac aggatccgcc
    15661 agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc aggactaaca
    15721 ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata tatcgaacgg
    15781 atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat ttattttcca
    15841 gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct atttggtctt
    15901 tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct gttgatcaat
    15961 tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg caagcttatt
    16021 cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga cttccaactg
    16081 tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc gggcttgcta
    16141 gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg agaattgaaa
    16201 aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat catgatcgtt
    16261 tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt cgcaggaaac
    16321 agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg aagactggtc
    16381 acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct tactggaatg
    16441 cgtctccctt atgttattgc aaaagaaaac aacctgcctt ttagcaagct ctttgctaaa
    16501 cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt catcgctatt
    16561 ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt tgtcatttgg
    16621 attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga acctgatttg
    16681 tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat cattggaggg
    16741 acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat ggggatagta
    16801 ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct aaaacattcg
    16861 aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat gaggagtacg
    16921 tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt ttgtctacga
    16981 tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt ttactttgat
    17041 ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac caagcaacca
    17101 gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt agacaaagca
    17161 tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag attgaagact
    17221 agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt agttcttttt
    17281 cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc aaaaacaaag
    17341 tacatattca gcatctcgac accccctttg tgtttatctt actttacttt gaaagaaatg
    17401 agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat aagagagata
    17461 caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt ttgcgctgat
    17521 tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg aatatcgaaa
    17581 gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt tttatttatt
    17641 gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg cccaaatgac
    17701 agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag acaacacggt
    17761 attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg tatttttgga
    17821 accagctttt aatgttttat tactcatacc atttggcatt tatttaagat attatttccg
    17881 gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt ttgaattgac
    17941 gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg atgtgaatga
    18001 tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt tatttacatt
    18061 tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa agactgtctc
    18121 attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc ttattaattt
    18181 ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt ctcatatctg
    18241 gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa ccaatggatg
    18301 gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg aacgaatttc
    18361 tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg gtttaagctg
    18421 gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt tgcagatgag
    18481 tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt taaatatctt
    18541 gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc acacatatac
    18601 tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga gcaaaaacgc
    18661 tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg cacgaatgaa
    18721 taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg caaagggctt
    18781 caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag gaaaaaatat
    18841 cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc ctttgtttga
    18901 aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa gtagggaact
    18961 agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac cggagatccg
    19021 tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc tgcatcacca
    19081 acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga atgaacaagc
    19141 gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga cattggatat
    19201 gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc tgcagttttt
    19261 agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat acatggaaaa
    19321 tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg aaaattggct
    19381 gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga caattggtca
    19441 tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa ttgccgatta
    19501 cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc caagagagag
    19561 aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag cgactgattt
    19621 agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag aaattccaga
    19681 agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct taaaaggaac
    19741 ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag aaaaattggg
    19801 cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat acgatcgtaa
    19861 tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga ttggtcttgt
    19921 gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt gggcaattgc
    19981 agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta ctgctcgtaa
    20041 aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc gtgtcacaat
    20101 ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat cttcaataaa
    20161 aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg tcatttcagc
    20221 tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag cttcaagcaa
    20281 ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt ccggcttgaa
    20341 gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt gatcaagaaa
    20401 tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg caggaatccc
    20461 ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta tccattcatc
    20521 cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag atgtattgat
    20581 tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat gaaaataaaa
    20641 attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga aaaggatatg
    20701 atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat agtcactaat
    20761 caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg ttatcgagtt
    20821 taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt tcgtccttgg
    20881 ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat gaaacaatta
    20941 agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg acatacgatt
    21001 gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat caactctggt
    21061 gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc aagaatcaca
    21121 aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac agcacggcac
    21181 catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa aaatgaagcc
    21241 atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga tccagaaaaa
    21301 ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca tgaagaagtt
    21361 ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat cggtgcaggc
    21421 ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa cgatttagca
    21481 gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga aatctggaac
    21541 ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact tcctcataaa
    21601 aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga tgcgtcaacg
    21661 aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct aagcaaacaa
    21721 gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc agatcatatt
    21781 cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg gagagggtat
    21841 gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg tattgaacaa
    21901 gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta ttatcctgaa
    21961 gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga acgtttcaat
    22021 gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt aaaaaacgaa
    22081 aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata tggcttcccg
    22141 gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca tgctggtttt
    22201 gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac tgaaaaatca
    22261 atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt cattggatac
    22321 acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc tcttttgaat
    22381 gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta tgcagaaatg
    22441 ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat cgtagcagtt
    22501 gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga agtaaaagca
    22561 ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg caatcggatc
    22621 atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt acttggcgag
    22681 catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga ctttacccac
    22741 tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa cgaaaaaatc
    22801 tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa aaacatgggt
    22861 gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa tattggcggc
    22921 tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat cggaatcttc
    22981 aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc tgtaacaagt
    23041 aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt agcaacaatt
    23101 gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca acagcaagta
    23161 agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca acaagctggt
    23221 gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc acaagtaaaa
    23281 gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa aaatatttca
    23341 gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc ggcaatgacg
    23401 aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat cgcaccgaaa
    23461 gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa aaatcctgct
    23521 ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata gggcgtttac
    23581 gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg ggcgaagaca
    23641 aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa atggtcggat
    23701 tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat aaataaaaag
    23761 gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg cgatcttgtt
    23821 agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat taggattcat
    23881 tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag gcccaaaaga
    23941 tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct atgccatgcc
    24001 aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag ttcccagagt
    24061 acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa ataaatcaat
    24121 aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag tcttagtgtt
    24181 aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg tagactctgc
    24241 tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag aagcaatagg
    24301 tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac cgttgaaacc
    24361 agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa aggaagagag
    24421 gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa ctagaagggg
    24481 taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa cagcggacaa
    24541 aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg tttgtctcta
    24601 agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca gtgaacagtc
    24661 tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct gcaaagccag
    24721 cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg ttatttgttt
    24781 cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa aaaatcatcc
    24841 atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt tccatctttt
    24901 tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat tctgaaacat
    24961 tgagagctac agcaacagat agttataatg atgtctttac tacactggct gtattgcttt
    25021 cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt tttgctttag
    25081 cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac gaactattag
    25141 gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat gcttgcgatg
    25201 atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa cgctttgcat
    25261 cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag attattgatc
    25321 tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac ttggatccaa
    25381 tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa attcttcttg
    25441 aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga aaaacgctgc
    25501 aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta gaaagaatct
    25561 tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg tttgatcaca
    25621 attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt taatttaaag
    25681 agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat ctgctccttt
    25741 atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata gaagttcttg
    25801 gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt cgaccgtaga
    25861 aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt cgtttttctt
    25921 tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg tccctataga
    25981 aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc cacctccact
    26041 aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa aaagagattc
    26101 tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc ggtgagaggt
    26161 tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat catctattgg
    26221 tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa cacaggtcct
    26281 tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct aggtttgcgg
    26341 cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt ttggaaagac
    26401 cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg ggtgcaaatg
    26461 atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga tcaagtagtc
    26521 ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta tccttatacg
    26581 aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt ttacaggaaa
    26641 agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt gtttatctat
    26701 tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc agcttgtgtt
    26761 cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa agttttgaat
    26821 ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc ataactgata
    26881 aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa agcactcacg
    26941 acctttctaa agtctgatca tagccttcat tataaagagt atttataagt acgtcaaata
    27001 aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata attggcatat
    27061 cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc aaatgaagaa
    27121 tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg ctagtaaatt
    27181 agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa tcaatttttc
    27241 tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta cttccatcaa
    27301 tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag acgcgctcaa
    27361 taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc ctgtgatggg
    27421 aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg aagatatcaa
    27481 cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat tatctgctct
    27541 tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc gagtgatttg
    27601 gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg gcttgggtgg
    27661 gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca gcgaaatcat
    27721 ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg caaggatcgt
    27781 tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag cagaaggcgc
    27841 tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa tttacggaac
    27901 accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca atagtatttt
    27961 agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag gatttggtgc
    28021 agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa aagcgccgga
    28081 tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa tgaaaaaaga
    28141 tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt taaagcgcca
    28201 tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg aattcgtaac
    28261 agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg ggattcttgc
    28321 caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag caaaagctgt
    28381 agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc tagaagaaac
    28441 gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg gtgttgtttt
    28501 cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg acaaactgcc
    28561 gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc taggtgcacc
    28621 ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag gatttttagt
    28681 agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg ctgcattgaa
    28741 tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat tataatctac
    28801 catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg aattaatttt
    28861 gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa agcataatag
    28921 gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat gggtagctcg
    28981 attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca aaagataacg
    29041 cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa aagccccgtt
    29101 tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt gattcttcaa
    29161 atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca aaatttattt
    29221 ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa aaaccgggca
    29281 tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca gtttcaatcg
    29341 aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat ttaccgaact
    29401 tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag atgatctttt
    29461 acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg attttgtcat
    29521 tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag aactgattca
    29581 gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac aagatcttcc
    29641 gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc cgttatacaa
    29701 taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc tagcaaaaga
    29761 aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga tcatccaaaa
    29821 cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg aggttgaagc
    29881 gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg ggaatcccga
    29941 tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag aaaaggattg
    30001 attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg gatcagttgg
    30061 tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt ataccaggta
    30121 ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta gaagggaaga
    30181 tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca ttgatgatta
    30241 aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt ctagctggtg
    30301 cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg ttccagacag
    30361 attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt ggagtgattt
    30421 gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga cagaacttaa
    30481 aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt tattcaccga
    30541 ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg tcaatggaca
    30601 gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct tgatccccga
    30661 accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt acgaagatga
    30721 agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag caggtcatac
    30781 gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta cgatcaacgg
    30841 cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac tgctaatggt
    30901 tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata agacctcttt
    30961 gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa ccatcaatgc
    31021 gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag atgggaagtc
    31081 ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg ttgaattaaa
    31141 actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg gttatccgtt
    31201 agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc agtttctcca
    31261 tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct ttgaagcacc
    31321 tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg cattttaatt
    31381 tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt cctttaagtt
    31441 tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc ataagtagat
    31501 tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta aacattcctc
    31561 ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga aaatgcaagc
    31621 aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta cttatgagat
    31681 tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa cacgaggaat
    31741 ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg atattcctgt
    31801 aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag aagaaccgga
    31861 acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt tgattgatga
    31921 tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg attttggcag
    31981 accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat tgccaatccg
    32041 tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt tagtagagat
    32101 gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt agaggagaga
    32161 gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat atccacgatc
    32221 gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg atgttcggag
    32281 caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta gtcagttcag
    32341 gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg tacttaggaa
    32401 gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt tatccagcga
    32461 ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg atcatcaaaa
    32521 agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt ccagtcatca
    32581 tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat aatcagggca
    32641 cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca attttcttca
    32701 acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt attagcggat
    32761 acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac gctgcctggt
    32821 ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta tatcttggag
    32881 caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc ggccatctta
    32941 tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt cataagacgt
    33001 tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct cctgtaacaa
    33061 gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg tttgtgatcg
    33121 gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc gctttgattt
    33181 taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga gtaattgccg
    33241 caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag aaaaatctgt
    33301 tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa gtaggcacat
    33361 ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat cttatcttac
    33421 cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc aaatcagaac
    33481 gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa gttatgggct
    33541 tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa aagcgccagt
    33601 actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc ttcgatgtag
    33661 cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc tctgtccaaa
    33721 aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta gacgtagcgg
    33781 ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa acaatccagt
    33841 gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc ttgcttgatt
    33901 tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc atcgtaggtg
    33961 atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt ctaggcgcac
    34021 aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg tacggacatt
    34081 atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt cgggtgcaac
    34141 acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag gaacatgggt
    34201 taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac ccagcaccag
    34261 ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg cggatcgttc
    34321 agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg ctaaatggaa
    34381 aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt cagatcaata
    34441 cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga atcaaagcaa
    34501 tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag ggacaattga
    34561 tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt acatacaagg
    34621 agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact gtatgcgcga
    34681 tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta tatgacatca
    34741 ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa aatctgcgaa
    34801 gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct ttcacaaatg
    34861 acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa gctgcaaaaa
    34921 acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc ggcgttatgc
    34981 acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta accgagtctt
    35041 ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac catgtttgcc
    35101 atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca ggaatccacg
    35161 taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt cccgaagatt
    35221 tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa gctttgatcg
    35281 aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc catggattag
    35341 aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc gaaacagcgt
    35401 ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa caagtgatca
    35461 attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg ctaacggttg
    35521 gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc gataaagaag
    35581 actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa ggcgaaacac
    35641 aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag tatggaacgt
    35701 ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc agatgctagt
    35761 gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac tatcactgat
    35821 caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa ttatggggta
    35881 aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt aaaagaacat
    35941 gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt gaaacaaaaa
    36001 ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat ccgtgaagtc
    36061 ggaacgatga aagctagtat cgttgatgct ggagattcct tcgaacatgc atttgatcag
    36121 ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa caaaccttat
    36181 ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa acatagtatt
    36241 ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa cacagatgcc
    36301 gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc tggtgatcca
    36361 aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt accgattttt
    36421 ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac ctataaaatg
    36481 aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg aagaattgat
    36541 tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc agaaaaactc
    36601 atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca cagagattat
    36661 cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga tgcactacat
    36721 ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac agatgccgaa
    36781 acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca ttggtcaagc
    36841 cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag aaggatatga
    36901 agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag tggcagatca
    36961 agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa aagagcgtcc
    37021 agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa tggagctggc
    37081 agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc tttctgcgat
    37141 cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga atcagccaat
    37201 ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa ataaaatcgg
    37261 ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag gtatgtgtga
    37321 aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc ccgctaccca
    37381 atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag tgatgagaga
    37441 ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg tgggtatcca
    37501 tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg aatatcaaat
    37561 gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag gctgtaatgt
    37621 ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca atccgcgtgt
    37681 ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta agctagctgc
    37741 taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag gaacgacttt
    37801 tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct ggccgtttga
    37861 taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg gtgaagtgat
    37921 ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt tagaaatcgg
    37981 tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc taatccaaaa
    38041 agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta gaagagggta
    38101 caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag ataaactgtt
    38161 gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca agatattgaa
    38221 aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg aaataaacga
    38281 agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata agatggttga
    38341 cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct atgaatatga
    38401 aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg gtcctatccg
    38461 tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag caatccagca
    38521 ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga ctgacttttc
    38581 tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga atgtgatcga
    38641 gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa tcaacttagc
    38701 cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag acttggatcg
    38761 tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc ctcaaccttt
    38821 aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa ttggttatcc
    38881 agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg ttgaaaacca
    38941 gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac atccagtact
    39001 agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg acggtgaaac
    39061 agtactcatc ccagggatca tggagcatat tgagcgtgct ggtgtccatt caggggattc
    39121 aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga tcgaagatta
    39181 cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc agtttgtcat
    39241 ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga ctgttccatt
    39301 cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta ttttgggaga
    39361 gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag aagttcatgt
    39421 taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact tagggcctga
    39481 aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag ctttgtacaa
    39541 ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat ttacgattgc
    39601 ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa ttggctatag
    39661 tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg tcgttgaaac
    39721 agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa taagagatgg
    39781 taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt ctaaagacgg
    39841 tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt ctttagacac
    39901 agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc ctatttaacg
    39961 gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca gatgaaacaa
    40021 gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga aatgacattg
    40081 aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat tctcgtgccg
    40141 cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca cgaaacaaat
    40201 acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc tactctttct
    40261 gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt gtccaatctt
    40321 catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc tctatatgag
    40381 ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg ttttgccaca
    40441 catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag aatcgctact
    40501 gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg aacgaagacg
    40561 gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt ggaacagctg
    40621 ttttcttcgc atcaaaatgt ccagttatca ttagaatccc gcatggcatg tgggataggg
    40681 gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa aagtgtgaaa
    40741 gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat gagaatcctt
    40801 tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca agcggctgtt
    40861 tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga tcgatcatgg
    40921 tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg gcggaaacac
    40981 catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt gttatgcata
    41041 aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat gttgctggtg
    41101 cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca aatgtgaaag
    41161 caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct tttggaacag
    41221 attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct gttcctgtct
    41281 atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca atcgaagctg
    41341 gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt gaccttaaga
    41401 caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca atcaagcctg
    41461 tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc attgggatgg
    41521 gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca agtgctgtag
    41581 ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt aaagagctcc
    41641 ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa gaagtgagag
    41701 aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag atttttcaac
    41761 taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat tgtttgtgaa
    41821 aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc taaaagaggc
    41881 tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg tcgaaaaagc
    41941 aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg ctggcggtat
    42001 tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag gtggaaaacg
    42061 tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa tgcatgccga
    42121 tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct gtgccaaaaa
    42181 agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca aagaaatggc
    42241 aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag tcgcaggaga
    42301 tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt ttatcgttat
    42361 aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga ttcaaacaga
    42421 atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat tagaaattga
    42481 agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca aaagcccaat
    42541 ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga tcgcaaaagg
    42601 attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg gaactgcaac
    42661 agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa tggtctatat
    42721 tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta tatttgaagg
    42781 tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct tggaagcagc
    42841 tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct ttacgtatga
    42901 gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga cgttgaccaa
    42961 ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc aagaattaac
    43021 tctgctaaaa gaatggaaaa aagatcctga aaattggcag atttaagcgc gcttgatgaa
    43081 gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa aatccaaaaa
    43141 ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg tgatcagttt
    43201 ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag aataatctct
    43261 tctctttttt ctattatata tagattttct ctattttttg gtaagggagc tgtttcaaaa
    43321 atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata ttcggaaaaa
    43381 gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag gattgaacaa
    43441 tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata cagttgcagc
    43501 acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag aagcgaatgg
    43561 acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact acacagagcg
    43621 ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa gagggatgct
    43681 gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc cagctgagtt
    43741 ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag aaggagaact
    43801 attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa tcatcaaaaa
    43861 taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga ttaaaagctt
    43921 tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac tattgaacca
    43981 ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa agaaagaaga
    44041 aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata tggatgtcga
    44101 atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg attggtttgc
    44161 gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg aagaaaccaa
    44221 aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg acaaggaatt
    44281 caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt tcaagaaaga
    44341 acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca tcaacggaaa
    44401 acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga atctagttgt
    44461 gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag aaaatgggga
    44521 aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct ttccggagca
    44581 aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg ggccaatcca
    44641 atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc ccttcgatgg
    44701 tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag aaatttatta
    44761 tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc tgtagcaaac
    44821 aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag tctttcagct
    44881 ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac gaatcgaggg
    44941 gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat attggtccag
    45001 cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt ttcgatcgct
    45061 gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga attcaaagac
    45121 gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga agaagtcaaa
    45181 gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc tggtatcaga
    45241 agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa aacgcatatt
    45301 tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga agaattgtta
    45361 tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt ttatgaagac
    45421 cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg tactttgatg
    45481 aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat tggagggatt
    45541 cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt ttatatcatt
    45601 tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga gcttactcgg
    45661 ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga acaccgttga
    45721 tcaaaaaaga ggggttgtga cagaattt
//
LOCUS       DACDYM010000015        45434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDYM010000015 DACDYM010000000
VERSION     DACDYM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.015"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            261..1130
                     /locus_tag="IWM59_000844"
     CDS             261..1130
                     /locus_tag="IWM59_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9978895.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1339..2364
                     /locus_tag="IWM59_000845"
     CDS             1339..2364
                     /locus_tag="IWM59_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9978896.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2403..3353
                     /gene="manA"
                     /locus_tag="IWM59_000846"
     CDS             2403..3353
                     /gene="manA"
                     /locus_tag="IWM59_000846"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9978897.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3447..5150
                     /locus_tag="IWM59_000847"
     CDS             3447..5150
                     /locus_tag="IWM59_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9978898.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5152..7185
                     /locus_tag="IWM59_000848"
     CDS             5152..7185
                     /locus_tag="IWM59_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9978899.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7196..7636
                     /locus_tag="IWM59_000849"
     CDS             7196..7636
                     /locus_tag="IWM59_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9978900.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7836..8963)
                     /locus_tag="IWM59_000850"
     CDS             complement(7836..8963)
                     /locus_tag="IWM59_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9978901.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8966..9643)
                     /locus_tag="IWM59_000851"
     CDS             complement(8966..9643)
                     /locus_tag="IWM59_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978902.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9907..11673
                     /locus_tag="IWM59_000852"
     CDS             9907..11673
                     /locus_tag="IWM59_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9978903.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11688..12125
                     /locus_tag="IWM59_000853"
     CDS             11688..12125
                     /locus_tag="IWM59_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9978904.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12138..13289
                     /locus_tag="IWM59_000854"
     CDS             12138..13289
                     /locus_tag="IWM59_000854"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9978905.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13351..14049)
                     /locus_tag="IWM59_000855"
     CDS             complement(13351..14049)
                     /locus_tag="IWM59_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9978906.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14042..15394)
                     /locus_tag="IWM59_000856"
     CDS             complement(14042..15394)
                     /locus_tag="IWM59_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9978907.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15617..17017)
                     /locus_tag="IWM59_000857"
     CDS             complement(15617..17017)
                     /locus_tag="IWM59_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9978908.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17178..17915
                     /locus_tag="IWM59_000858"
     CDS             17178..17915
                     /locus_tag="IWM59_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9978909.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18040..18519
                     /locus_tag="IWM59_000859"
     CDS             18040..18519
                     /locus_tag="IWM59_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9978910.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18516..19283
                     /locus_tag="IWM59_000860"
     CDS             18516..19283
                     /locus_tag="IWM59_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978911.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19513..20082
                     /gene="lepB"
                     /locus_tag="IWM59_000861"
     CDS             19513..20082
                     /gene="lepB"
                     /locus_tag="IWM59_000861"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9978912.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20186..20788)
                     /locus_tag="IWM59_000862"
     CDS             complement(20186..20788)
                     /locus_tag="IWM59_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9978913.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20894..21363
                     /locus_tag="IWM59_000863"
                     /pseudo
     CDS             20894..21363
                     /locus_tag="IWM59_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21469..22053
                     /locus_tag="IWM59_000864"
     CDS             21469..22053
                     /locus_tag="IWM59_000864"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9978914.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22056..23129
                     /gene="prfA"
                     /locus_tag="IWM59_000865"
     CDS             22056..23129
                     /gene="prfA"
                     /locus_tag="IWM59_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9978915.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23122..23961
                     /gene="prmC"
                     /locus_tag="IWM59_000866"
     CDS             23122..23961
                     /gene="prmC"
                     /locus_tag="IWM59_000866"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9978916.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23954..24133
                     /locus_tag="IWM59_000867"
     CDS             23954..24133
                     /locus_tag="IWM59_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978917.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24155..25180
                     /locus_tag="IWM59_000868"
     CDS             24155..25180
                     /locus_tag="IWM59_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9978918.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25355..26599
                     /locus_tag="IWM59_000869"
     CDS             25355..26599
                     /locus_tag="IWM59_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9978919.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26752..27699
                     /locus_tag="IWM59_000870"
     CDS             26752..27699
                     /locus_tag="IWM59_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9978920.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27833..28462
                     /gene="upp"
                     /locus_tag="IWM59_000871"
     CDS             27833..28462
                     /gene="upp"
                     /locus_tag="IWM59_000871"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9978921.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28601..28843)
                     /locus_tag="IWM59_000872"
     CDS             complement(28601..28843)
                     /locus_tag="IWM59_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978922.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28964..29036
                     /locus_tag="IWM59_000873"
     tRNA            28964..29036
                     /locus_tag="IWM59_000873"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28997..28999,aa:Thr,seq:cgt)
     gene            complement(29155..29793)
                     /locus_tag="IWM59_000874"
     CDS             complement(29155..29793)
                     /locus_tag="IWM59_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9978923.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29916..30548)
                     /locus_tag="IWM59_000875"
     CDS             complement(29916..30548)
                     /locus_tag="IWM59_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9978924.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30899..31198
                     /locus_tag="IWM59_000876"
     CDS             30899..31198
                     /locus_tag="IWM59_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9978925.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            31693..33858
                     /gene="acm"
                     /locus_tag="IWM59_000877"
     CDS             31693..33858
                     /gene="acm"
                     /locus_tag="IWM59_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9978926.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(34130..34615)
                     /locus_tag="IWM59_000878"
     CDS             complement(34130..34615)
                     /locus_tag="IWM59_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9978927.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            35095..35736
                     /locus_tag="IWM59_000879"
     CDS             35095..35736
                     /locus_tag="IWM59_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9978928.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            35755..36390
                     /locus_tag="IWM59_000880"
     CDS             35755..36390
                     /locus_tag="IWM59_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9978929.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            36408..37223
                     /locus_tag="IWM59_000881"
     CDS             36408..37223
                     /locus_tag="IWM59_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9978930.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            37542..38978
                     /locus_tag="IWM59_000882"
     CDS             37542..38978
                     /locus_tag="IWM59_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9978931.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            39167..41614
                     /locus_tag="IWM59_000883"
     CDS             39167..41614
                     /locus_tag="IWM59_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9978932.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            41844..42911
                     /gene="rlmN"
                     /locus_tag="IWM59_000884"
     CDS             41844..42911
                     /gene="rlmN"
                     /locus_tag="IWM59_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9978933.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(43104..44207)
                     /locus_tag="IWM59_000885"
     CDS             complement(43104..44207)
                     /locus_tag="IWM59_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9978934.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            44760..45308
                     /gene="aac(6')"
                     /locus_tag="IWM59_000886"
     CDS             44760..45308
                     /gene="aac(6')"
                     /locus_tag="IWM59_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9978935.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        15538 a         7788 c         9277 g        12831 t
ORIGIN      
        1 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag gaggagctat
       61 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctttttata
      121 agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt
      181 ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga
      241 cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa
      301 gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg
      361 gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa
      421 gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa
      481 agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc
      541 gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga
      601 ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg
      661 accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa
      721 aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact
      781 taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag
      841 agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag
      901 gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg
      961 atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct
     1021 tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg
     1081 tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt
     1141 cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga
     1201 gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg
     1261 tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata
     1321 gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa
     1381 gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt
     1441 acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt
     1501 gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa
     1561 gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag
     1621 acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac
     1681 agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag
     1741 caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta
     1801 gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta
     1861 ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa
     1921 ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct
     1981 gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa
     2041 atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat
     2101 tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta
     2161 aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt
     2221 ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct
     2281 gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa
     2341 gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa
     2401 ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc
     2461 gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga
     2521 tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat
     2581 tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc
     2641 cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg
     2701 atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg
     2761 atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag
     2821 ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag
     2881 gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt
     2941 tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg
     3001 cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg
     3061 cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt
     3121 atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca
     3181 aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg
     3241 acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg
     3301 agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa tagttcccag
     3361 atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat
     3421 caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc
     3481 gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa
     3541 atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg
     3601 atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct
     3661 tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa
     3721 ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta
     3781 tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat gtgaaattca
     3841 aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt
     3901 gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct
     3961 ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac
     4021 ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa
     4081 tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac
     4141 ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg
     4201 cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg
     4261 tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga
     4321 tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga
     4381 tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta
     4441 catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga
     4501 acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt
     4561 ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat
     4621 tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc
     4681 aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac
     4741 ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa
     4801 taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag
     4861 aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac
     4921 aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta
     4981 tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa
     5041 gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac
     5101 attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta
     5161 tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc
     5221 caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat
     5281 cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt
     5341 gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt
     5401 aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca
     5461 gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat
     5521 gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac
     5581 cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta
     5641 gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat
     5701 tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa
     5761 gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg
     5821 ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga gacatataat
     5881 agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct
     5941 gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac
     6001 ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat
     6061 ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat
     6121 gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta
     6181 caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt
     6241 gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa
     6301 gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc
     6361 aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta
     6421 cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta
     6481 aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga
     6541 tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta
     6601 aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat
     6661 aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat
     6721 caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg
     6781 attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat
     6841 cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg
     6901 ttattccata catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa
     6961 catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt
     7021 atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga
     7081 gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt
     7141 tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat
     7201 acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt
     7261 gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc
     7321 aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca
     7381 tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga
     7441 tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc
     7501 tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat
     7561 cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga
     7621 acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac
     7681 ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat
     7741 gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac
     7801 aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata
     7861 gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc
     7921 atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt
     7981 tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa
     8041 gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga
     8101 gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac
     8161 aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga
     8221 ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt
     8281 atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta
     8341 aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag agagattacc
     8401 tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc
     8461 aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc
     8521 gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac
     8581 tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga
     8641 tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg
     8701 ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat
     8761 tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta
     8821 tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca
     8881 aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg
     8941 cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa
     9001 tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag
     9061 aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt
     9121 tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca
     9181 acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat
     9241 gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca
     9301 atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca
     9361 aataatcaac agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag
     9421 accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag
     9481 ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg
     9541 caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt
     9601 ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat
     9661 aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg
     9721 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
     9781 cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt
     9841 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
     9901 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
     9961 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
    10021 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg
    10081 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
    10141 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
    10201 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
    10261 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
    10321 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
    10381 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
    10441 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
    10501 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
    10561 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
    10621 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
    10681 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
    10741 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
    10801 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
    10861 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
    10921 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
    10981 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
    11041 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
    11101 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
    11161 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
    11221 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
    11281 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
    11341 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
    11401 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
    11461 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
    11521 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
    11581 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
    11641 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
    11701 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
    11761 gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag
    11821 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
    11881 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
    11941 tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag
    12001 tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca
    12061 atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg
    12121 cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat
    12181 ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca
    12241 cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa
    12301 aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag
    12361 taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac
    12421 cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa
    12481 catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag
    12541 aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg
    12601 cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg
    12661 aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa
    12721 aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca
    12781 atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg
    12841 aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa
    12901 gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa
    12961 tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac
    13021 cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac
    13081 ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc
    13141 ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat
    13201 tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg
    13261 tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa
    13321 atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa gatagggcag
    13381 atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag ctgccaggtt
    13441 ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc ggtaaatgac
    13501 accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc ccaaaggtcg
    13561 ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat aagtttcatt
    13621 gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct gacttaacgt
    13681 gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca tgtagtagtg
    13741 acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt cgatatagtt
    13801 gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct caaagtcttg
    13861 accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat gtatactaac
    13921 gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca tcaaaaggtt
    13981 tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat tgagttcata
    14041 attagccacg gttcattcct cctttgataa atccttgagc agctagttct ttacgaagct
    14101 gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat tctttgattt
    14161 tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga atcccggcaa
    14221 cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca gctgggatat
    14281 ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg attccgtctg
    14341 cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga ccaatcatat
    14401 cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat tttttcccat
    14461 tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa ccttgtttga
    14521 ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa gcattgtaga
    14581 cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg aaatctgctg
    14641 acacattgtc catccgtacc atatcagtta atcgataatc caattcagga cgtttgaagt
    14701 cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta taatgcaaga
    14761 tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt tgttcacgat
    14821 cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata gaattgaaga
    14881 ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca ccgtctacaa
    14941 tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg aaaatatttg
    15001 taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt gcttcatcta
    15061 tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag aaagtcgata
    15121 caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg aattgctggc
    15181 gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg gtgacaacga
    15241 cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc agcgctagtt
    15301 tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc gacgtttttc
    15361 caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa acgatttgct
    15421 gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt gcttttctta
    15481 ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac
    15541 aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat tattccctct
    15601 tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca tagcctagat
    15661 ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc cgcccaataa
    15721 acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta atccctaaag
    15781 ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc agcgtttgtt
    15841 cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa agcataaaga
    15901 caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata ttgatgttat
    15961 cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta gttgttcgaa
    16021 tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa aaaatgatca
    16081 gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta cgagtcgtaa
    16141 tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg aataaatggt
    16201 ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca aaagctaaca
    16261 cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa cgtttgtata
    16321 atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa
    16381 acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa tccccagtga
    16441 cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa aaaacggcat
    16501 cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa
    16561 gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg ataccagaca
    16621 aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt
    16681 tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca acttgaaata
    16741 atatttccag cagtataata ccgttatcat tgaacacaga attgatatca aaagtcgata
    16801 gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa gaaacatggc
    16861 tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc gtcatcaata
    16921 tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc
    16981 gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc tgacaatagt
    17041 cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca aacagataac
    17101 aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta gaaaaaagaa
    17161 ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa
    17221 aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa
    17281 agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca ttggaattca
    17341 tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat cctaaaagtg
    17401 agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat gagaaaactg
    17461 accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc aaaatggttt
    17521 taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata
    17581 tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac tttggaagtg
    17641 ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga atggatatcc
    17701 aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac
    17761 gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt ggttgcgtgt
    17821 tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca aaagcttact
    17881 taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga
    17941 aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc gtagtaaagt
    18001 aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt agcacctatt
    18061 ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac aggtattgtg
    18121 aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga aacaacacca
    18181 actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga aaccactacg
    18241 acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata tacaactgac
    18301 agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc aaccaatcca
    18361 agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac gactgtagat
    18421 gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat
    18481 acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta gaccttatca
    18541 acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt aaccgtggat
    18601 atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat ggactttggc
    18661 aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg cttgtttatt
    18721 ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc gatgtcactc
    18781 agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa gaatcgatag
    18841 ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct tatgacgaag
    18901 attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt gagatcaaaa
    18961 caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg tatccttatt
    19021 actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga agctactcgg
    19081 acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg gaacctgtcg
    19141 gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat gagccttatc
    19201 gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt tatagtcgta
    19261 gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa
    19321 acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt ttttatataa
    19381 cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa cattgtagaa
    19441 taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga aaatacaagt
    19501 aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct tgtattagcg
    19561 gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg
    19621 gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa ctatgaacga
    19681 atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt taaacggatc
    19741 atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat taatggcaaa
    19801 aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga taagttacaa
    19861 ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc cacctttacg
    19921 agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga taaccgcttg
    19981 atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca aggaaaagtt
    20041 gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag
    20101 ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat ttcttggagt
    20161 tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag aaaagcgaag
    20221 cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta gcagagacaa
    20281 agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta tggactttcg
    20341 gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg ccaggagcta
    20401 aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa agtacaagtg
    20461 atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca gcgtggatcg
    20521 ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc agcatagttt
    20581 ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa ctaacaagcc
    20641 cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt agataagtag
    20701 ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca taatggttag
    20761 cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac tttgaaattt
    20821 taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca aaaagggaga
    20881 atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt
    20941 ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata
    21001 tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag aatatttagg
    21061 ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta ttgtatggga
    21121 ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga agcagaaggt
    21181 tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg gcactactct
    21241 atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt ggaagttttt
    21301 ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag
    21361 tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta aaaaatgctg
    21421 aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat ggcacaacta
    21481 ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa agtcgcacat
    21541 aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga tacaagagat
    21601 ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa
    21661 gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg tgtgctgatc
    21721 gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat tgtggacgaa
    21781 ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga gttatttgaa
    21841 ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg
    21901 ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc tgtttatgaa
    21961 ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg aaaacattac
    22021 tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta caagcaattg
    22081 aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt tctgatacaa
    22141 agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc gaagtttatc
    22201 gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt ggtgaaaaac
    22261 tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag
    22321 aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat gacgataaaa
    22381 atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta ttcgctggcg
    22441 atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg
    22501 aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc accggagaca
    22561 acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa
    22621 ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg ccagaagctg
    22681 aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat catgcttcag
    22741 gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact cacttaccaa
    22801 cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt gaaaaagcaa
    22861 tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa agcgagtatg
    22921 atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc cggacgtata
    22981 atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa aaattagatc
    23041 agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat gatcaaacat
    23101 caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct gtcacgggct
    23161 tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt atttttggaa
    23221 cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat ttcagaagaa
    23281 gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc acctcagtat
    23341 ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc aacactgatc
    23401 cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg
    23461 ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt gaaagcagca
    23521 agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga agtagcgaaa
    23581 gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac attaggacca
    23641 gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag tcggaatgaa
    23701 tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct ttttgctgag
    23761 aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt actaaaatca
    23821 aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa aaaaatattt
    23881 cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg caatgaacga
    23941 atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt tttttattat
    24001 aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac aagtttatcc
    24061 actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac tcagacaaaa
    24121 taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat atgatgaaac
    24181 agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt ttcctaccga
    24241 aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga aagtgttttc
    24301 tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt ttgaacaagt
    24361 aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga acttctggcc
    24421 tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag ttgtgacggg
    24481 aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag aagtaatcga
    24541 actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac caagtccaac
    24601 gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta ttgatgatgg
    24661 agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa cagccatgcc
    24721 tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc
    24781 tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa
    24841 atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg attggtcaac
    24901 tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat
    24961 tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca actcagtaga
    25021 agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa cacttggatt
    25081 agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt acatgaatcg
    25141 cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt attgtcgcat
    25201 tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga aaaactaagc
    25261 gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga tgggaaaaat
    25321 aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa cgtttgaccc
    25381 tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt tggagctgat
    25441 tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta tcttgacaaa
    25501 taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat ttgtcgatat
    25561 cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat ttgcgaacgt
    25621 ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg tagaaccagg
    25681 ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg gatcaccagt
    25741 caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga caacagaagt
    25801 tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat tgatcgtagc
    25861 aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa tcgcagatga
    25921 agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag cagcaggact
    25981 tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata aaacattgcg
    26041 tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa aaataaatag
    26101 tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg gtaaagcagt
    26161 agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa tcatcgccaa
    26221 tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca tttcaggggc
    26281 gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg gaaaagaagc
    26341 agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc cttttgaaac
    26401 actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca ctactcgcgg
    26461 gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat tacaagcaaa
    26521 agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa ctgaaaaatt
    26581 tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt tcaatgttgg
    26641 gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga tgctctctag
    26701 tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt catgactgat
    26761 cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc tcatccgcgt
    26821 atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca agaatcacct
    26881 gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga atggggaaaa
    26941 ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt tattttttat
    27001 attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt gatcagagaa
    27061 cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc accagaagca
    27121 aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa taaattagcc
    27181 aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt aggtggtaac
    27241 atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat atccaagcaa
    27301 ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca tgaattcgga
    27361 gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata tacgacgaat
    27421 gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa tgaactaaat
    27481 gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga tgaaggtgag
    27541 gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg cttccaagga
    27601 atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac gcgtgcagca
    27661 atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt ccgcaggaaa
    27721 acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag tcaatcactt
    27781 gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa aaatgggcaa
    27841 atttcaagtg atcgatcatc cattgattca acacaaacta actattattc gggacaaaaa
    27901 ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga tggcatatga
    27961 agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca ctgaaacaac
    28021 acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg caggtattgg
    28081 aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata tcggtcttta
    28141 tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag aagacattga
    28201 tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag caatcatggc
    28261 aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt tggtagcagc
    28321 acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct atacagcttc
    28381 tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg atgcgggtga
    28441 ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac ttttgtcaca
    28501 tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc tttcgtttgc
    28561 atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt tttagttctt
    28621 tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt tctgcagttt
    28681 catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca gtttgcgact
    28741 tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca atttccgatg
    28801 gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct gcctttcaca
    28861 tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc catcggactc
    28921 tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct tagctcagtt
    28981 ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag ctggcattaa
    29041 aagagaagac agatatatca aggattttta atccattgat acatctgtct ttttgtttaa
    29101 aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa aaatttagga
    29161 gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg cccaacttgt
    29221 aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca ccccttgtat
    29281 caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac tgtaatggat
    29341 aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact tactagaaaa
    29401 aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag gcaaaataat
    29461 aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc atttcaaaat
    29521 ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa aatatttggc
    29581 tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt ttttaccaaa
    29641 aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa taatggatgg
    29701 cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata tccctttttt
    29761 aagttctatt ttttttccta aaatgttatt catgataata actcctacta ttcagttttt
    29821 gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta tggatatttg
    29881 tattgctaaa aatttatctt atatattcat tttgattata ctacttttat ttgtaaaaat
    29941 tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag atgttccgag
    30001 cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa atcagtatca
    30061 aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag ggaacagtct
    30121 acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat gtttgaacca
    30181 gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat ataactttcc
    30241 atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa catgcttttt
    30301 gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat ctccattctt
    30361 tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat aattggatgg
    30421 cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag ttcaaaatct
    30481 tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt aactttagga
    30541 aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc taaaccaact
    30601 tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt gtcgctatct
    30661 cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc taataaaaat
    30721 cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa aatcgttgcc
    30781 ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat ctagatgtag
    30841 cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg aaatcgtgat
    30901 gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg ttttaaatcc
    30961 agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct tagaaaaagg
    31021 tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt tagcaatcag
    31081 gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa taggaaaatt
    31141 tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc atttataaaa
    31201 acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc aggctaggtt
    31261 catttatttg aacagagtct gtattttttt attctaaata aagaaacaga tgtttcttta
    31321 tattgtattt tatcctttaa ttaacacaaa aacagctagt ataaactaat aatgttaatt
    31381 aaaagtgtat tttatgaaac tatcgttatt tattaatgtt gcatttttgt aagttaaaaa
    31441 ttggaaatat agattgaaaa aatgaaagca ataagtaaaa taaaatgaat atatgcgaaa
    31501 aaataattta cattaggaaa aaccttgtaa aaacagtttt ttggcatatt tattttttat
    31561 aaatatttgt aaaataacta tcaatttttt agttaaaaga ttggaaatga gtgggggagg
    31621 aattgaagaa aggatttttg agagatgata tagtaggttt tatttttagt taaaataggg
    31681 gagatagaaa aaatgaaaaa gtgtagaaat attattttat caatgttatt tattattact
    31741 aatataacat cactgattcc tgttcatgtc tatgcggatg caggcagaga tatcagcagt
    31801 aatgtcactt cgttgaccgt ggacccaacg aatattacag atggaggaaa cataaaagta
    31861 aaattctcgt ttgacgaaaa aaaacaaaat atccaaccag gtgattatct ttggatcaat
    31921 tggccaagtg aaggaaatat tcgcggtgaa ggattccaaa aagaaatccc attaatgatt
    31981 gaaaataaaa atgttggaac tttaacggtc agaaaagatt ctgcgcaggt tgtgtttaat
    32041 gaaaatatta aaaacctaga ctcagtagaa ggttgggggc aatttgagat acaggccaga
    32101 aacgtaaccg ataccggtga agaaaatacg ggaccattca ccgtgacgag cggtgataaa
    32161 acagctactg taaatgtgac aaagccagct tctggttctt cttctagcgt tttttattat
    32221 aaaacaggag acatgctacc tgaagatact aagcacattc gatggttttt gaatattaac
    32281 aataacggca cttatgttga acaaccagtt aaaatcagtg atgaaatcca aagtggacaa
    32341 agattagatc caagtacgtt tgaaattaat caaatacact taggtgaaca gaaggtttat
    32401 agaggagaag agggaatcca acaattttta caagatttcc ccagcgctac atttaatttt
    32461 tcagtaactg ataattacat tgaaataact ataccaaaaa actttgtcaa tcttaggaaa
    32521 ataatggtga gttataagac gatcatagaa aacccagaac aaataaattt tgagaatcat
    32581 tcggaagctt ggtttaaaga atttaataaa cctgctgttg atggagaaag tttcaatcat
    32641 acagttaaaa atatttcagc ttctggtgga gtcaacggta cagttagagg cgaattgaag
    32701 attttcaaat atatcaatga cacagagatt ggtatcccaa atgtgacttt cgaattaaga
    32761 cgagcagatg aacagcctat tcaaggacag tctagtatcc tgttgacttc gaatgaacag
    32821 ggagaagcta gtatcaaagg cctgcaagta ggagactatg ttgtaaaaga aaaagaagca
    32881 cccaattgga ttgattttga tccactaagt tcaaatgaac tcaaattctc cataaacgaa
    32941 aatgatacag agggagtcag tttacctatt tataacaaga aaaaggttac gaacatcaca
    33001 gcaactaaaa tttggaacgg tggaaccact ccaagaccga gtatttattt taaattattc
    33061 agagcaagta caaacaattg ggaaccagtt cctgatgcag aaacaaaacg attagacaat
    33121 ggaataactt cagtgacttg ggaagatatc caacagtatg atgatagtgg aaacgaatat
    33181 acgtttaaag tgcaagaagt agatcaaaac ggaaatgatt atgtcccttc aggttatcaa
    33241 aaaattgaaa acgggctagt cgttacaaat gagaataaag aagtcatttc agtctcagga
    33301 caaaaaacat gggaagacaa tgagaaccaa gacggcaaac gaccgacgac gatcacagta
    33361 aatctattag ccgacggtca accgatccaa cacaaagaag taagtgaaaa agatgattgg
    33421 cgctatcgtt tcacaaatct gccaaaatac agagacgggc aagaaatcat ctatactgta
    33481 acggaagaca acgtgcccga atacagtacg acgattgacg ggtataacat caaaaacagt
    33541 tatacaccgg aaaaaacaaa tatatctata ccaggtagcc atataacacc gtggaaaaat
    33601 tttccagaga agacagagag agagaaaatt agaaatctat tctctcaaaa attgcaaata
    33661 tttgctcata caggagaatc cagcgaacaa agaagcaatt atattagtaa gagagctgta
    33721 cctaaacaga taacaaataa atcaaaaagc attcttccta aaacaagtag taaaaaatct
    33781 agttttgctg taatcattgg tttattgata gtaactatct gtgctgggat attcttacaa
    33841 aaatataaaa aagtttagtt agaatataat attttttgtt aaatatgact gaaatcaaaa
    33901 gaataaaatt ccgatttata atcgaaaaaa gaagcatttt tatatatgct tcttttttcg
    33961 tagaatcaat aaatatggat ttggctaact atggaagaat actcgtatta gttttcagcg
    34021 ttcatactag ttgttacatt gtttgattgg ccaaaagaat ttctgatata ttttgtgcta
    34081 aataaagaaa aagacgcttg aattgggaaa taagaaaact actctatcat tacaaatttt
    34141 cataaactgg cagatgttct aagtaaacaa gtatcttttc aaagtcggaa aaatccataa
    34201 tcaacgtagt ggtattgata tttggatgaa agcctacggt atcttgacga tcaatagcag
    34261 catcgatcac gacttggatt tttttctctt tgtcgtgcat caatgctaat ggagtcactg
    34321 tacctgctgg aacgcctagg aggtcaggaa gctgttcttc agaaacaaaa gataagcgct
    34381 tttcttttaa ttgttcagct agttgtttta aatcggcgtg cttttcatca ggcaagatga
    34441 caagatagat ttgttttcct tttcttgatt tcaataatag attttttact tgcggaccag
    34501 gtagggaaaa agggacattt ttgactgacg taatcgcagg atgatcgact ttttgaaaag
    34561 agatacccag tttttttagt agttcataag cttcttgttc ggttgcacga ctcatcgcgc
    34621 atcaccctcc tttttttatt agtgtatcat aaaaaagagt tgacaaatga aaaataatca
    34681 tgtaaattac acgtaaagtt aaaacaaatg acatcgaaaa gaagagtagc cgttaggtgt
    34741 ttttagagag tctctggttg gtggaaagag gcagcagtta accgtgaaac acatcttgga
    34801 gtcgattttc tgaagcgtat agtaggaaaa tccggggagc ccgttacagc tgcggtcgtt
    34861 gctagatgac cagataaggc tggcttttcc gtgaggagtc ggtaaaaaaa ggtggaacca
    34921 cgtgattaac gtcctttgat cttcggatca aaggactttt tgttttcatc tgaacggcaa
    34981 cgactgtgtg aggttttttc tgcaaggaaa aaataaacag aggtggaacc acgtataata
    35041 cgtcctcttg gcatttgcca agaggatttt tttattttaa aggaggaaaa gaagatggag
    35101 tatttgattg aagttatgcc gcaattatta gtaggagcac tcacaacatt gaaattattt
    35161 ttatttacct tgattggctc attaccatta gggattgttt tagcattagg attgtctttg
    35221 cattttaagc cgctacgcta cttgcttcaa acatatgtat gggttatgcg gggaacaccg
    35281 ctgttgttgc aactgatttt tattttttat gggttgccga cgattgggat cgtttttgat
    35341 cgtttcgaag cagcactcat tgcctttatc ttaaactatg cagcttattt tgcagagatc
    35401 tttcgtggag ggatacaatc gattcctcaa ggtcaatatg aagcagcgca agttttgcaa
    35461 ttaagtccct ttcaaacggt gacgaaaatc gtcatcccac aagtgacaaa gattgtttta
    35521 ccttctgtag ggaatgaagt tatcaacttg gtcaaagatt cttccttgat ctatatatta
    35581 ggtttaggtg acgtaatgcg tgctgggaaa atcgcgatgg aacgcgacgc gactttactg
    35641 cctttgctag gcgtggcaat catctatctt acattgactg gtattctaac aatcgtaatg
    35701 aaacaagttg aaggaaaatt aaattattac cgctaactga taggaggaag aaatatgatt
    35761 gaattgaaga aagtgaacaa agcatttggt gaaaaaaaaa taatcaaaga tttgaatcta
    35821 atcattccag atgggaaaac attagctatc gtaggtcctt ccggtggcgg taaaaccacg
    35881 cttttacgta tcttggctgg cttagaaagt tctgacagcg gagaatttta tttagacggt
    35941 gtaccgttca acccattcga gcttaaaatg aaagaacaag ttgtaggagt cgtattccaa
    36001 gacttccagc tatttccaca tctgaatatt tttgaaaaca ttaccttagc accaaaaatg
    36061 gtattgaaac agacaaaaga agaatacacc aaaaaagcag aagaattagc ggcttcatta
    36121 gggattagtg atttattgaa tatctatcct tatcagcttt ctggcggaca aaaacagcgg
    36181 gtagcgattg ccagagcatt ggctatgaat ccacgggtat tggcttatga tgaaccaaca
    36241 agtgctttag atcctgaatt acgccagcaa gtcgagcgtt tgatcctatc attgaaagaa
    36301 gatggggtaa cccaaattat tgtcacccat gatttagtat ttgcagaaaa tgtcggtgat
    36361 caattactaa aagtttcccc aattcattaa gaaaggagaa gatagagatg aaaaaaacca
    36421 gttttctatt cgctttgatc gcaggtcttc ttctttttgc aggctgttcg aacaaaaaaa
    36481 cttcagcaga taattgggcg actatcaacg aagagaaaaa aatcgttata ggtttagacg
    36541 atacatttgt tcctatggga tttagagata aagacgggaa gctcacagga tttgatattg
    36601 atttagccaa agcagtcttt gaacaatatg ggattacggt agactttcag cccattgact
    36661 ggtcaatgaa ggaatttgaa ctgaacaatg gaacgatcga tttgatatgg aacggctatt
    36721 ccaaaacgcc ggcaagagaa aagaaagtgc aatttacaaa accttatatg gaaaacgatc
    36781 aagtgctgat cacacctaaa gaatcaggaa tcacaaactt tggacaaatg aataataaga
    36841 gattaggtgc gcaaaatggg tcatctggtt atgatgtatt tacgaaacaa ccagaagtat
    36901 taaaagagat agtcaaaaac caagatgctg ttttgtatga tagttttaat gaagcattga
    36961 tcgatctgaa aagcagacgg gtcgatggtc tgctgatgga taaagtatac gcagactatt
    37021 atttgaaaca aagaaacgaa ctagaagact tcaacataac aaaaggttct tatgagagtg
    37081 aggattttgc agttggtgca cgcaagtcag atagtacatt agtgaaaaaa atcaatgaag
    37141 ccctagatca actagagaaa acaggtgaat tccaaaaaat atccgataaa tggtttggcg
    37201 aagatgttac gccgagaaat taacaaatag gggactgtga caaaagacgt gtcacagtcc
    37261 ctttttccga ataaacagtg ttctcctgcc aactccttgt gactcggagc tgaatggcag
    37321 gctcacaacc tcttggtaaa cggtgttcaa aagccagttt ttcggtatta agccaaattt
    37381 atgcaaaaat gtagaaagcc attttcacaa aatttttctt actactcgaa actaagcgac
    37441 tttttcacaa cctcctttac atatttttat tttgttgtgg aaggggtttc gtgttattct
    37501 aaaagaggaa cgattgaagg ttataggaaa ggaagagatg aatggaacac aaagaactta
    37561 cctcatttcc aaaagatttt ctgtggggct ctgcttctgc agcgtatcaa gtagaaggag
    37621 cttggcaaga agatgggaaa ggagaatcag tctgggatcg atttgtacgg atacctggaa
    37681 agacatttaa aggaacgaac ggtgatttgg cagtagacca ctatcatcgt tataaagaag
    37741 atattgcttt aatgaaagaa caaggtctga aagcttaccg cttctctgta gcttggacac
    37801 ggatttttcc taatggacga ggagaagtca atcaagctgg gctcgctttt tatgagagat
    37861 tgatcgacga attgattgaa aacgatattg aacctatgtt gactttatat cattgggatc
    37921 ttccacaagc attgcaagat gaatataatg gttgggaatc acgtcagatc attgaagatt
    37981 tcactcatta tgcagaaaca ttatttgagg cattccgcgg gaaagtccat tactggatta
    38041 gtttgaatga acaaaatatc ttcacttcat taggttattt gttagcagca catccgccag
    38101 gtgtgacaga cccgaaacga atgtatgaag ttaaccatat tgctaattta gcaaatgcat
    38161 ctgtcatcaa taaatttcac gagttagaaa tgcctggaaa aatcggacca agctttgcgt
    38221 atacgccaaa ttatccaatt gacagtaatc ctgaaaatgt tctggctgct gaaaatgcag
    38281 aagacctgat ggccaattat tggatggatg tctatatgtg gggaaaatat ccgattgctg
    38341 ccatgaggtt cttagaagaa aaaggatggg caccaacgat tgaagctggc gatgcagaat
    38401 tgctagaatc tgctaaacca gattttcttg ggatcaatta ctatcaaacg gcgacgaatg
    38461 cattcaatcc attaaacgga gttggtgctg gaaaaatgaa tacgacagga aaaaaaggtt
    38521 catcagaaga aactggagtt cctggaatgt ataaaaaagt tgagaatcca tttgtcgaac
    38581 gaacaaattg ggactgggag attgacccag aaggattaag gatcggactg cgtcgtatca
    38641 caagccgtta ccgcatgccg gtactgatta cggaaaatgg cttaggagag tatgacaagt
    38701 tgacagaaga taaacaaatc catgacgact atcgaatcaa ttacttacaa agccacgtca
    38761 aagccatcaa agaagcaatt tcggatggag cagaagtcct aggctattgc acctggtctt
    38821 atacagattt gcttagctgg ctgaacggtt atcaaaagcg atatggattt gtatatgttg
    38881 atcaagacga aacacaagaa ggatctttgg aacgatataa gaaagacagt tattactggt
    38941 accaaaaact gattgaagaa aatggagaga acctttagtt cacatactta aaacaagaag
    39001 gtacggctgg atatggccgt gccttttctt gataaaatca tttagaaatt aagaaaaact
    39061 gtaaaataag ctgaaaaatg aaagaaagcg tcagttcttt caaacaaaac tttagttccc
    39121 ttactttttt ttgtataatg aaacttgatg aaatgaggga taataaatgg cacaaaagaa
    39181 acggcctact aaaaaaagga aaaagacgaa aaaaacgaaa aagcaaaaac agcagcagga
    39241 acaaatgctg acgattggtg ttgggtttgc cttgatttta tttgctgtct tcggcttttt
    39301 aaaattaggt tttttaggaa tattgattgc aaacggcttt cggatcattg caggaaatac
    39361 ttaccaaatc ctctgtcttt tattagccgt attaggattt tggattgtca ttaagaatac
    39421 agagttttca attggtaaaa accgacgctg gtttggcggc atactctttt atttcggtat
    39481 cttattattg ttacatgccc atttatttgg caaactccat actggggaac caaatatcat
    39541 ggggacgaca tgggatttgt tagccagtga tataaaacaa agtcaagtag acaataacgt
    39601 tggtggtggg atgattggag caatccttta tcattttact tacttcttga ttgcacagcc
    39661 tggaagttat ttagcagcag tgctattact ggcaggagga gcattcctgt tcagtaatct
    39721 ggaaggctat cagctgttaa atggtatcca atcaattgga gaacgagtac aggaattatt
    39781 ggaaggtgat cctgccaagc aagctcgaaa gcaagcggca aaggaagaga gaatgaaaca
    39841 gcgcgcagaa gcgaaagaag caaggcgctt agctgcaaaa gaagctgcag aaaaagaagc
    39901 tgcagaatat gagaagaaaa aagcggtgag ccagcaaagg gaaaatactg ctgatgaatg
    39961 gcaggaacca gaaaatacag agccggagca gctaagtttt gttccaatcg acagttttca
    40021 agaaaatatc catcctgcga atctagagaa accagtacca gatacaccaa aacagaccaa
    40081 tacagcagaa ggtttttcag atgagttgcc agaggatgac ggtacctcat tagaatttga
    40141 gattgaagca gaacaggaaa atcaagacta tgaattaccg acagttgatt tgcttgattc
    40201 tataccaact gtggatcaaa gtgatgagta taaaaaaatt gaaaaaaata tcggcgtatt
    40261 agaacaaact ttccaaagtt tcggtgtcga tgccaaggtc gtcaaagcca gcttaggacc
    40321 atccgtcaca aaattcgaag tccagccggc tgtaggggta aaagtaagta aaattgtgaa
    40381 cttaacagat gatatcgcgc ttgctttagc ggcgaaagat gtccggatgg aagcaccgat
    40441 tccaggtaaa tcactgattg gtattgaagt accaaatgga aagatcagta tggtttcctt
    40501 tagagaaatc atcgaagcac aaccaaacca cccagataaa ttactagaag tacctttagg
    40561 acgtgatgtt tctggacggg tacaaacggc tgatctttcg aagatgcctc acttactcgt
    40621 ggctggttcg actggtagtg ggaagtcagt agcaattaat gggattatca caagtatatt
    40681 aatgcgtgct aagccccatg aagtaaaact gatgatgatc gatccgaaaa tggttgaatt
    40741 gaatatgtac aacggcatac ctcatctgtt gacgccggtc gtgacgaacc ctagaaaagc
    40801 agcgcaagcc ttacaaaaag tagtacaaga aatggaagaa cgatatgaaa aattcgcggc
    40861 aaccggagta agaaatattt ctggttacaa tgaatttgtt caacagaaaa acttggaaaa
    40921 cggaacgaaa catccaacct taccattcat cgtagtgatc gtagatgaat tagctgattt
    40981 gatgatggta gccagcaatg aagttgaaga tgcgattatt cgtttagcgc aaatggctcg
    41041 ggcagcgggg attcatatga tcctagctac tcaacgtcca agtgtggatg ttatcacagg
    41101 aatcatcaaa gcaaatgtac cttccagaat ggcatttgcg gtatcaagcg gtacagattc
    41161 tcggacgatc attgattcta atggggcaga aaagctctta gggcgaggag atatgctatt
    41221 cttgccaatg ggtgagaata aaccgatccg tgtgcaagga gcattcattt ctgaccacga
    41281 agtagaacgg gtcgttcaat ttgttacaga tcagcaagaa gctcattatg aagagaaaat
    41341 gatgccgaca gatgaagttg aaacgacagg tgctccagaa cagccacaag atgagctgtt
    41401 tgaagaagca aaagcacttg tcgttgaaat gcaaacggca agtatttccc ttctccaaag
    41461 aagatttcgg atcggttaca accgagcagc acgtttagta gatgaactgg aagctcatgg
    41521 agttgttggc ccgtcagaag gaagcaagcc tcggaaagta ttcattgagc aggaagaaac
    41581 atcagaaacg ttgccggacg ataatgacat gtaaaacttg tgtgatgaag ggttgaaaaa
    41641 cgaataacca ataaaaagac aaggcatgat gataccaaaa gaaaaaatgg tttttttcca
    41701 tcttttctaa tacggtatga tgtcttgtct ttttagtttt tgtgagatta aagttctatt
    41761 cgatttattt gtgaagtatt gcattaagga aatgaatcat gtatagtatg tagcgaaact
    41821 ttgagaaaaa gagggaaaat actttggaaa aacagtccat ttatggcttg acgaatgaag
    41881 aattaatcaa ttggtttata gaaaacggcg agaaaaaatt ccgagctgcc caagtttggg
    41941 aatggttgta tcaaaaacgt gtaagtaatt tcacagaaat gacgaatcta tcgaaacaat
    42001 tgatcgaaaa attatcggag cattttatca tcaatcctct aaaacagatg gtcgtccaag
    42061 aagcttcgga tggtacagta aaatatttgt ttgaactccc tgataaaaac atgattgaga
    42121 ctgtactgat gagacaagaa tatggcttgt cagtttgtgt gacgacacaa gtaggatgta
    42181 atatcggctg tacgttttgt gcaagcgggc tgctcaaaaa gaaccgtgat ctgactgctg
    42241 gagaaattgt tgcacagatc atgatggtcc aacattattt tgatgagcgc aatcttggcg
    42301 aacgcgtttc acatgtcgtt gtaatgggga tcggtgagcc atttgataat tacgacaatg
    42361 taatggactt tttgcatatc atcaatgatg ccaaaggatt ggcaattggt gctcggcaca
    42421 ttacagtttc cactagtgga ttagcacaca aaatcaagga attcgctaat aacggcttgc
    42481 aagtcaatct ggctatttcc ttgcacgcgc cgaataatga ggtgcgaaca tcgatcatgc
    42541 ggatcaaccg aagtttccca attgagaaat tgatggaagc tgtggatgag tatttagaga
    42601 aaacaaaccg tcgaatcacg tttgaatata tcatgttgaa tcaagtaaat gatagaccag
    42661 agcatgcgca acaattagca gatctattaa aagataagaa aaaattagca tatgtcaact
    42721 tgattccata taatccagtc agtgaacatg atcaatattc tcgcagtcca aaagctgatg
    42781 tattgagatt ttatgatgta ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac
    42841 atggtacaga tattgatgca gcttgcggac aattgcgaag caagcaaatg aaaaaaacag
    42901 ttgccaaata atcgtatcct actcgttttt tcaattacca taaaaggagc agttttctta
    42961 attaaggaac tgttcaactt aaataagaga aaagattcta tagcgaaagc tataaagctt
    43021 tttttgacaa gaaaaaccct catcaagcaa agaaatgtcc tcaatttgat gacagcctgc
    43081 ttgatggggg ttttagcaat tatttaactg attttcttat atccttgctg gctttcttgt
    43141 gtagcatgat aagtttctac gtcaagaatg gcctgtttaa atttttctcg ggtgatttca
    43201 taaggcacat gtttaatatc atttgttgcc atggctttat ctaagattgc ttctaaatcg
    43261 gtcgtgacat tgagattcat gtcggttaac gatactggaa gttggttctt tttcataaac
    43321 acatagattt tatcaaacaa atcgaactgc tgatccatca tcaataaggt cagtacacca
    43381 taactgacag atgctccgtg aaggtatccc tccatttttt ctagaacagt cgtaccgtaa
    43441 tagaatgagt gtgccatagc agaattatag ttattgttaa ctaaaacaga aaccaatccg
    43501 gtagtaacaa tgatatccga aacgacttgt tcaattgcat atgagggacg atcgtcggca
    43561 ctatctttca atgcttgcgt accataagtc aagagacgat caatgcacat ttttccgatt
    43621 tggattccta atctatccgt gtagcctaga tcatcgccac gagcagaaaa agtagattcg
    43681 acttctttgg ataaagcatc gccaatacct gcccataagt atttatcagg tgcttcggca
    43741 ataattgctg tatcaatgaa ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc
    43801 attacgcctt cttcgttata gatgacacat accgcagtga cgggcgaaca gttcgaacca
    43861 atagtgggaa aggtgaagac tggtttagct agtttttcac caatgacttt tacagtatcg
    43921 caagcacggc caccaccgac tgcaaacagg acgtctgctt tttggacact ttcatgctgg
    43981 atcaactgtt caatatttcc ataagttgaa ttacccccgt accagagact ggcgacaacc
    44041 tctatttcct gtgtcaaagc tacttttatt ttgttttctg cctttgaaag tgcagtcttc
    44101 cctccaatca gcactacttt tgtcccatat tctttgacga ttccatttgc tttttcatac
    44161 gcatctgttc caatggtata atttggtaag aaaacacttt gactcatatt catttccttc
    44221 tttcttcgtt gaaacgggtt aaaattttaa agagtgatcg atctgactgc ggctgtagat
    44281 ggagtagagg tgaacctaag acaggataag tgatgaataa ggtggcatga acaccttgaa
    44341 atcatcaata agtatcctaa aaacgaacaa gtacaaaaac attgaaaacg acaataatga
    44401 atttttgttc ctcttagtat ttctaccgat tccctttgct tttttgtaat tatctaaaaa
    44461 ttctaaaaat taagattatt atatgaaaac aggttttatt tgtcaattca taattggttt
    44521 tttttaaagc aaaagataat ctatatcatg atgaacaaac agagtttttt cctccaattg
    44581 atggctttcc tgatctttcc tattttgaat aaagtgtttc tgtataattt ttttatttta
    44641 taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga tttttgagcg
    44701 agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg ggaagacgta
    44761 tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt tctgatttac
    44821 tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta gaagaaatga
    44881 tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta ggatttattg
    44941 gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt gtagaaagct
    45001 cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa gaagtagctt
    45061 ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga acaacgttaa
    45121 gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag aaccttcgtg
    45181 aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc ttaccaaatg
    45241 caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct cgaccaaatt
    45301 ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa gaaggaattt
    45361 acgaaaatta cttcttgaat ttttgtgaaa ttccaactta tttccgaaga agctactttt
    45421 ggaacaccgt gtac
//
LOCUS       DACDYM010000016        44938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDYM010000016 DACDYM010000000
VERSION     DACDYM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.016"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="IWM59_000887"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="IWM59_000887"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9978936.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="IWM59_000888"
     CDS             1011..2258
                     /locus_tag="IWM59_000888"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9978937.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="IWM59_000889"
     CDS             2334..2678
                     /locus_tag="IWM59_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978938.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="IWM59_000890"
     CDS             complement(2795..3121)
                     /locus_tag="IWM59_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978939.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4695)
                     /locus_tag="IWM59_000891"
                     /pseudo
     CDS             complement(3494..4695)
                     /locus_tag="IWM59_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4812..5327
                     /locus_tag="IWM59_000892"
     CDS             4812..5327
                     /locus_tag="IWM59_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9978940.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5526..5972)
                     /locus_tag="IWM59_000893"
     CDS             complement(5526..5972)
                     /locus_tag="IWM59_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9978941.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6019..7497)
                     /gene="msr"
                     /locus_tag="IWM59_000894"
     CDS             complement(6019..7497)
                     /gene="msr"
                     /locus_tag="IWM59_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9978942.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7851..8480)
                     /locus_tag="IWM59_000895"
     CDS             complement(7851..8480)
                     /locus_tag="IWM59_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9978943.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8464..8928)
                     /locus_tag="IWM59_000896"
     CDS             complement(8464..8928)
                     /locus_tag="IWM59_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9978944.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9415..10683)
                     /locus_tag="IWM59_000897"
     CDS             complement(9415..10683)
                     /locus_tag="IWM59_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9978945.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10914..11651)
                     /locus_tag="IWM59_000898"
     CDS             complement(10914..11651)
                     /locus_tag="IWM59_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9978946.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11718..12287)
                     /locus_tag="IWM59_000899"
     CDS             complement(11718..12287)
                     /locus_tag="IWM59_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9978947.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12415..13287
                     /locus_tag="IWM59_000900"
     CDS             12415..13287
                     /locus_tag="IWM59_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9978948.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13629..14501
                     /locus_tag="IWM59_000901"
     CDS             13629..14501
                     /locus_tag="IWM59_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9978949.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14498..16456
                     /locus_tag="IWM59_000902"
     CDS             14498..16456
                     /locus_tag="IWM59_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9978950.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16707..17558)
                     /locus_tag="IWM59_000903"
     CDS             complement(16707..17558)
                     /locus_tag="IWM59_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9978951.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17681..18148
                     /locus_tag="IWM59_000904"
     CDS             17681..18148
                     /locus_tag="IWM59_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9978952.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18411..18893)
                     /locus_tag="IWM59_000905"
     CDS             complement(18411..18893)
                     /locus_tag="IWM59_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9978953.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18993..19856)
                     /locus_tag="IWM59_000906"
     CDS             complement(18993..19856)
                     /locus_tag="IWM59_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9978954.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20454..21302
                     /locus_tag="IWM59_000907"
     CDS             20454..21302
                     /locus_tag="IWM59_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978955.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21473..21814)
                     /locus_tag="IWM59_000908"
     CDS             complement(21473..21814)
                     /locus_tag="IWM59_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP9978956.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21900..22607
                     /locus_tag="IWM59_000909"
     CDS             21900..22607
                     /locus_tag="IWM59_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9978957.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22594..22917
                     /locus_tag="IWM59_000910"
     CDS             22594..22917
                     /locus_tag="IWM59_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9978958.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23006..24043)
                     /locus_tag="IWM59_000911"
     CDS             complement(23006..24043)
                     /locus_tag="IWM59_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9978959.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24490..25473)
                     /locus_tag="IWM59_000912"
     CDS             complement(24490..25473)
                     /locus_tag="IWM59_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9978960.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25889..26659)
                     /locus_tag="IWM59_000913"
     CDS             complement(25889..26659)
                     /locus_tag="IWM59_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9978961.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26652..27587)
                     /locus_tag="IWM59_000914"
     CDS             complement(26652..27587)
                     /locus_tag="IWM59_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978962.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28181..29830)
                     /locus_tag="IWM59_000915"
     CDS             complement(28181..29830)
                     /locus_tag="IWM59_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9978963.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30025..30921)
                     /locus_tag="IWM59_000916"
     CDS             complement(30025..30921)
                     /locus_tag="IWM59_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9978964.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30914..31594)
                     /locus_tag="IWM59_000917"
     CDS             complement(30914..31594)
                     /locus_tag="IWM59_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9978965.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31595..32377)
                     /locus_tag="IWM59_000918"
     CDS             complement(31595..32377)
                     /locus_tag="IWM59_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9978966.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32370..33287)
                     /locus_tag="IWM59_000919"
     CDS             complement(32370..33287)
                     /locus_tag="IWM59_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9978967.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33290..33574)
                     /locus_tag="IWM59_000920"
     CDS             complement(33290..33574)
                     /locus_tag="IWM59_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9978968.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(34402..35523)
                     /locus_tag="IWM59_000921"
     CDS             complement(34402..35523)
                     /locus_tag="IWM59_000921"
                     /inference="COORDINATES: protein motif:HMM:NF019371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HAP9978969.1"
                     /translation="MAKLKLQLEEEERVNQVSEELSEEIKDLCKKAKNKEIFSITDQI
                     KYARFKGIEISESDESIVQDILLYRTYYFKVTAYRKNFEKDINGKYIDLSFVKLADLA
                     KIDMYLRMILSRMIFELEHSLKTLIISSITNSLTEDGYKIVEDYDMYMQNKFIDSQRK
                     KGNTKTNEEILSEYTQASEKILDKVKGKFGYDFDFYNHHHDKISIWALLEVMTLGNLE
                     RFITFYFEEEHFGYLGLKTAQKLLKYVTNVRNASAHSRPLIYNIVEPYQFGRADSNLK
                     KRRPVIQLTQFATNAGLDSELIARALTNMKMNDIVATIYLHEKYVQTQKLKTTNFEAL
                     SDLLDRIKVNGKMYEKNHELVEVFHFFEKIVMEYGKLIT"
     gene            complement(35573..37189)
                     /locus_tag="IWM59_000922"
     CDS             complement(35573..37189)
                     /locus_tag="IWM59_000922"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002010966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9978970.1"
                     /translation="MSNASEITAKLWAMANKLRGTMDASEYKNYILPFMFYRYLSENQ
                     DEYLEKNGLDEYYEVTDSEEKEDYLEEISRGIGYAIAPEYTWEQLVSKIENHQIKASD
                     FQDMLDSFNLNAKRNPVAEDDFANVFSDINLGDTRLGSNTNERAKALNDVVLMVNDFT
                     FKDDSGHDILGDVYEYLIGQFAANAGKKGGEFYTPHEVSQVLAKIVTKDAKADDTQFR
                     VYDPAMGSGSLLLTVKNELPDGNKQGSVDFYGQELNTTTYNLARMNLMMHGINYRNMN
                     LKRGDTLDADWPFAEKDGMQIPLKFDAVVANPPYSAKWDIKNVDREKDTRFKGYGVAP
                     ASKADYAFVLHGLYHLDKTGTMAIVLPHGVLFRGASEGRIRKNIIDNNLLDTVIGLPA
                     NLFYGTSIPTAVLVFKGRESRRTKDILFIDASSEFVKGKNQNKLSPENIDKIIETYEK
                     REDVEKYAHVATLDEIKENDYNLNIPRYVDTFEEEEVTPLPHLAQELADVKQEIEKST
                     DKLFEMLGELHGTTDEAQEELRKFMEMLGR"
     gene            complement(37205..38419)
                     /locus_tag="IWM59_000923"
     CDS             complement(37205..38419)
                     /locus_tag="IWM59_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9978971.1"
                     /translation="MSNEITKEPEIRFDGYEGDWEERKVGEVFSERSERSGEGELISV
                     TINSGVVKASELDRKDNSSDDKSNYKKVEVGDIAYNSMRMWQGASGYSPYNGILSPAY
                     TVIIPKKDIDSKFFAYDFKRYDMIQTFKRNSQGLTSDTWNLKFPTLKSVKVMVPSIEE
                     QKQISTFFESLDTTITLHQRELDLLEQTKQTYLKKMFPKTGEDKPEIRFAGFTDAWEQ
                     RKLGEVATRVTRKNKKLESTLPLTISAQFGLVDQNTFFNKQIASKDVSGYYLLKKGEF
                     AYNKSYSNGYPWGAVKRLDKYDMGVLSTLYICFRPVDINSDFLASYYDTDRWYKEVSK
                     HAAEGARNHGLLNIAASDFFETELMIPKNNNEQEKIADFFKSLDTTITLHQRELDSLK
                     EMKKTLLQKMFV"
     gene            complement(38416..39417)
                     /locus_tag="IWM59_000924"
     CDS             complement(38416..39417)
                     /locus_tag="IWM59_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HAP9978972.1"
                     /translation="MNEIQFLIYEDSDKVEVLIREETIWATQKTISQLFDVGVPAISK
                     HLKNIFESGELDEEVVVSILENTTQHGAIDGKTQTNKVKYYNLDAIISIGYRVNSQKA
                     TKFRIWATDILKDYIQKGFKIDVERMKQGENAFGKDYFRELLETVRSIRASERRIWQQ
                     ITDVFAEISYDYDKNSEITKQFYATVQNKFHYAITGKTAAEIVFNGADKTKENMGLTT
                     WKNSPDGRILQSDVSVAKNYLSEKQIRSLERNVSSYFDYVERLLEDETLLGMEDFAKS
                     IDEFLTFNRYQILDGNGKISSKAAKRKAVDEYKEFNKHQHIVSDFDKEITKIEGELK"
     gene            complement(39424..42480)
                     /locus_tag="IWM59_000925"
     CDS             complement(39424..42480)
                     /locus_tag="IWM59_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002087288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9978973.1"
                     /translation="MKSQSELRFEQEVVDYLTRIGGVKQWEYKAEIKTTEQLWNNFKK
                     ILEQNNQARLDEPLSATEFNQVKKIITNINTPYQAGQFLYGVNGVSEIEVDLDNGKHV
                     FLTVFDQAQVGGGNTVYQVVNQIQRPKVVDGKPNRRFDITLLINGLPIIQIELKKALH
                     NATEALNQMEQYIAEKQFSGIFSTLQILIAMTPFDIRYMANTTLQNFNRAFAFNWQDE
                     ETAHPVRSWKLFADKVLSIPMAHDLSTRYMVLDGTKNKESIKVMRPYQVYATKRVLDK
                     VRKFDFKHDEGKLGYIWHTTGSGKTITSFKTAWLASRLSNVDKVIFLVDRIALTNQTA
                     DAYRAYDPIAGFEGKTGVVSDTANISDLHRKLTKKSDKNIIVTSIQKMSRYVTRDSFQ
                     PLTENVLFIVDEAHRSTGDGTKNEGMLESIRKALPNSAWVGYTGTPKFPETREIFGDL
                     LHAYTIKEAIADKNVLGFKVEFKETIEAPADPTEEDIDDNLRGSVYDHSPEHVELVVE
                     DIFDNWKERSNNRKYNALFTVHVGGNRASTPRAMEYFDKFMEENNKRSIEDQIKVAIS
                     FSMDTSNSSHQLKTNENLHRAIQSYNRLFGTAFDMTTVKSYTEDLARRLNKTADDGNY
                     LDLVIVVDQLLTGFDAPELNTLYIDRTLKGGNLIQAYSRTNRIHDRDAKPQGNIVNYR
                     WPIQNEYEMNKSFAIYSNRDSAAEQLSLDELKKDNEESGILSKSFSKIESETKEVIQK
                     LAELTDNFIQLPPSEKAQEEVFEGLQEYNRLLNQLKQFTEDDEKNPVSVYDEPELFYD
                     RLGITEDQEVMLTTVLANELRERRAQIENIDISQVNLEMTHIHDVTINYDYLIELIAR
                     MADEVHEDKMDQAELTKNEIDVEIAKSDSDKEITKVRNFVSKIFTKEFIFDKYPAPRD
                     VEEMNQAMEQSQKDSNIKLYTEFVRTWGLDNSVKPKDLAKLISRHRVGQEDMDKQGEL
                     TAMMNDARTDYKEIAAAEVANLSWVKYRIEFRKAFYQMADETKKGE"
     gene            complement(42502..43080)
                     /locus_tag="IWM59_000926"
     CDS             complement(42502..43080)
                     /locus_tag="IWM59_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9978974.1"
                     /translation="MEMRKLSEFVTFVQGINQSRAEKQFGNQEIDYYDQASFEEDHKY
                     YNEATQEERVNYLFDQEVSLDKRDVVISNSLQRATMVSGQNVGKVLSLNFTKVEFKSD
                     LLDKRYFLYLFNNHKDIQRQKDRGLQGTGPVLRLTKQSLEQLVIPLPLIEEQRKIGGI
                     YTESLKLQSKLSIYTRLLEQFTNSMLEKSLEE"
     gene            complement(43383..43628)
                     /locus_tag="IWM59_000927"
     CDS             complement(43383..43628)
                     /locus_tag="IWM59_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9978975.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRIRLEKGIGQTELIRDLQLHKIA
                     ITRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            43733..44506
                     /locus_tag="IWM59_000928"
     CDS             43733..44506
                     /locus_tag="IWM59_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978976.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSETSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAKLYTEKVEQFFKSGANKDFSKISDYSENFFNETQDEIMNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLEKPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEVEMVIKIF"
     gene            complement(44736..>44938)
                     /locus_tag="IWM59_000929"
     CDS             complement(44736..>44938)
                     /locus_tag="IWM59_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prevent-host-death protein"
                     /protein_id="HAP9978977.1"
                     /translation="LQEVVHSNSEIEITRDSKNGLNDGVIMISKRKWELLQEELYLQR
                     TGTWAYVSDLMKNEKEDDFKVV"
BASE COUNT        13405 a         9314 c         7642 g        14577 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gttttttcaa
     4441 accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta
     4501 actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt
     4561 taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac
     4621 tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt
     4681 ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtaa
     4741 atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag
     4801 gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac
     4861 gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga
     4921 tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg
     4981 agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat
     5041 ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg
     5101 ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc
     5161 tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac
     5221 aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc
     5281 aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa
     5341 cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg
     5401 aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta
     5461 gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg
     5521 ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg
     5581 atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag
     5641 aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc
     5701 attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt
     5761 actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg
     5821 aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga
     5881 tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta
     5941 aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg
     6001 aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt
     6061 tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa
     6121 aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat
     6181 aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat
     6241 cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg
     6301 agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct
     6361 catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac
     6421 ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa
     6481 taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa
     6541 ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc
     6601 catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac ggatttgttt
     6661 catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta atgttttcgc
     6721 attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca accgatccgg
     6781 tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg cttgtttttt
     6841 cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact ctgcttctcg
     6901 tagctgagtc tttttctcca attccttttg agtgagatag gcactataat tccccgaaaa
     6961 ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat ctagaaaata
     7021 gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt caatcagtcg
     7081 atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc cttgttcata
     7141 agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc atttccgctc
     7201 tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta ggtcctttgc
     7261 ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt ggattttccc
     7321 atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac cgacaccatt
     7381 ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa tagatagttc
     7441 tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat tttccatatg
     7501 ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga gcagaaaaat
     7561 ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa ctatgactaa
     7621 atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa tggacatact
     7681 ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt tattcaaaag
     7741 ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta ttaggttacc
     7801 acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt ttattcgaac
     7861 gtagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac cgcttttcct
     7921 attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc ctctgccaac
     7981 tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg cgcaactaac
     8041 atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat ttcttctctt
     8101 gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac ttcttttggc
     8161 atcttccctg attcgtaagc ttggttatag atttcttcgc catattcgta acattgcata
     8221 tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc
     8281 aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg ccatggttgc
     8341 atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat ttcttcgtgt
     8401 gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat aattgttgaa
     8461 aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta tattgcaaag
     8521 ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat cgctgaaagc
     8581 cggggatagc acggaacctg tatctttatg gtccatttca caatgctcga ttttcttctg
     8641 ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc tctgacgcga
     8701 aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt gatcaatctg
     8761 caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc tcatcatgat
     8821 ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga ttttttgatc
     8881 ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa tttcctcctt
     8941 tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa agaaaaaagt
     9001 caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa
     9061 ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa ccaagattat
     9121 tgattcttat caaactatag aagatagctc ttctgcagta gaaaatttta caccgccaaa
     9181 aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc
     9241 ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg agtaaacggt
     9301 gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt
     9361 tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct ctttttaata
     9421 accataaaat cctattcctt catatctcca gcccatcggt gttagccaat ctctttcctt
     9481 agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata gtcgataaat
     9541 tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga tttttgttaa
     9601 agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag gattgtataa
     9661 acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat attcccagcc
     9721 tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt ctcctgtatg
     9781 acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat actgtgtcag
     9841 attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag tggcataacg
     9901 acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc atgcaccgga
     9961 atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa
    10021 tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc attgcccatt
    10081 cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt taatcccaaa
    10141 tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt caacgatggc
    10201 ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt gtgcagttga
    10261 ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat aaaaagcata
    10321 gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc
    10381 atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag cttctgctga
    10441 tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt cggttgaaat
    10501 tgtattttct gagacagtag ctgactgcaa agtactctct gtatcgtttt caattcctgt
    10561 ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag ctagcactgt
    10621 tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat tctttgcttt
    10681 catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt
    10741 aaaagcaagt caatgtccat atttaaataa catttgtttc tttttgataa atttgctttt
    10801 tctacataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta aattgatggt
    10861 cgtttgaata actatttaac aagtaattct ctttggatat tatccgttat ggattatact
    10921 tcctgtgtcg tcggacggat aatcatgtta ctgacagaca tccggtctgg cgtttctatc
    10981 gcaaatgcta cagcagaagc tatatcttcg gaggtcaatc cccattcttt ttgcgcttca
    11041 tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt ttgaacagct
    11101 cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg taatccttcc
    11161 atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg ataaacaaca
    11221 tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc catgattgga
    11281 agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat attttgccat
    11341 tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt attgtacaat
    11401 acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa
    11461 tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc ttttattgct
    11521 tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc tgctaatagc
    11581 ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac tttttctgat
    11641 aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac tcactatctc
    11701 ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt ggcagggatt
    11761 cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc tgcaacaatg
    11821 gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt
    11881 ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg
    11941 ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca
    12001 cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt
    12061 ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta
    12121 atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct
    12181 gctaaaggct cttcgttttc agcttggatc tgatggatca cttcaaagag cggatccgtt
    12241 actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct
    12301 tttatcctta ctttataacg gacggcttat catgtctaat acttataaaa catacttaac
    12361 tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat
    12421 attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca
    12481 aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa
    12541 cttggtgtga cacttttcac tcggggctcc cgagagatac agttgacgga ttctggtcga
    12601 tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta
    12661 acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc
    12721 tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat
    12781 ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt
    12841 ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa
    12901 gatcgttggg gacttttgat acatcgaaat catcctttag caaagcaaac cgtcatccat
    12961 ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa
    13021 ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta
    13081 ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg
    13141 atcatcaata cttatgggac ggaaaaaata ttcatcccac tcgatccgcc attacaagct
    13201 tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta
    13261 gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta
    13321 acaacgaata agataagcaa aagatccaac gattaaataa cataagtttc ttaatttatt
    13381 tcttcctttt atttctgata attccagtaa attactataa ttataaaaaa agaattgaca
    13441 aaacataact gtctatataa aattgattac gttaacaata attcgtttca tgtgactaga
    13501 tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt tttcgtgtct
    13561 atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc ttcaaaaagg
    13621 gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta gatgacggtg
    13681 gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga aatgatttga
    13741 tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa atcaagttgc
    13801 aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg atcattgatc
    13861 atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct ttgaccgacc
    13921 acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac aaacttttaa
    13981 gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca gtagagtatc
    14041 tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc gcaatccata
    14101 ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa gtgacgattg
    14161 tatctgacat catccattta gtggaatcag aattggaaac agatatccat tcagaaccat
    14221 tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa cgaacccatg
    14281 cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga aaatacccag
    14341 aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac cagctcgcga
    14401 tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga agcgcaatcg
    14461 tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc tacaacgaat
    14521 gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac tagtagggat
    14581 cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac aagctggcgg
    14641 taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac tagggatgtc
    14701 agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag ttccagtaaa
    14761 tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat cagttgatcc
    14821 atcttttgct cagatcagta acgtatttat tggtattatt gccggattga ttgcggcagc
    14881 tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct ttagcggcaa
    14941 gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag tgctactgct
    15001 cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt tgagtttagg
    15061 ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta caggattgca
    15121 tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata ttggtaaatt
    15181 tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat ttttcccaat
    15241 catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac gtccagaaaa
    15301 caaagcaaaa acagcttcct tgatgctagc agcagctttt gcttcattct ttactggtgt
    15361 gacagaacca cttgaatttt catttatgtt cgttgcttgg ccattatatg tcttgcatgc
    15421 aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag caggatttgc
    15481 ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg cgaatcagcc
    15541 attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg gctttaactt
    15601 tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc tttcagatga
    15661 cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca aaatctacgc
    15721 tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac gccttcgttt
    15781 gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg gcgtacctgg
    15841 tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag tacaattcgt
    15901 tgcagatgag atggcaaaac tacatggtgg tgctgcagca cgacctgcac aaacaaacac
    15961 cgtggttaaa acagaaacat ttgccggtga aacgatcgaa caagatattt acgctatcgc
    16021 aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg aaaaaatgat
    16081 gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa tcgctggtac
    16141 gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg ctggtatcga
    16201 agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat tcactttata
    16261 tgtagaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg atcttgccgc
    16321 tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg gcgacaaagt
    16381 ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa tcggctctgt
    16441 ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa caaacaggag
    16501 ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt ctcctgccaa
    16561 ctccgcggta ataagtccag ctaaacgaaa catatgaaga gctatttttt atttgactct
    16621 tcttattact tgatgcagaa cagctttttc acaaaatttt tcttcatact caaaactaat
    16681 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
    16741 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
    16801 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
    16861 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaactct
    16921 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
    16981 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
    17041 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
    17101 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
    17161 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
    17221 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
    17281 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
    17341 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
    17401 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
    17461 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
    17521 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
    17581 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
    17641 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
    17701 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
    17761 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
    17821 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
    17881 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
    17941 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
    18001 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
    18061 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
    18121 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
    18181 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
    18241 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
    18301 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
    18361 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
    18421 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
    18481 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
    18541 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gcttacaaca
    18601 agatacgttc cttttgggaa tgggatgatt cgcgtagtgt ttggaacatc ctgactcgtt
    18661 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
    18721 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
    18781 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
    18841 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
    18901 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
    18961 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
    19021 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
    19081 tcgactaacc cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
    19141 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
    19201 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
    19261 atcaaactgc ccttttcttc caagcggtct ccatcaaatc cataagaaac gttgattact
    19321 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
    19381 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
    19441 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
    19501 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctactgatt
    19561 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
    19621 tatccatatc taaacgccgt ttctgtcact cttgatccat ctagaagatc gcgattagcc
    19681 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
    19741 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
    19801 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
    19861 atcttcctcc ttgattgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
    19921 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
    19981 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
    20041 aaattttggg gaagacagat tctaagtaaa caaagcggta aaaatatctt ttattccctt
    20101 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
    20161 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
    20221 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
    20281 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
    20341 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
    20401 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
    20461 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
    20521 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
    20581 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
    20641 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
    20701 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
    20761 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg cactttatcg
    20821 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
    20881 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
    20941 tcatggagat gcgaaagttc atccaagaag cagttgaatc gcttattcag caaaatgcct
    21001 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
    21061 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
    21121 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
    21181 tcgatcatac tgacgcctct tattcaaaac gctactctat aaaaatcctg aaagaagact
    21241 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaacta aagaaacggt
    21301 aaagaaagac gatttcaatg aaaaatagca agtaaacgat aacaaccatc ctaaatccac
    21361 aagaaataca gcagaccttg ctttaaaaac acgaatcatg caaaggatga agtaaagaag
    21421 acttaacgcg tacccaatat tagtcatttg actattaggt acgcgttttg ttttataagt
    21481 catgacaaat ttccttcgtt gctggataga ttttccggca ttgttgatag attttatgat
    21541 acttttctac attttgtaag agaggttccg ctgcatctgt atattggata aaattctgac
    21601 aacaactttc aggtgtgtcg aaccacccta accctagtgc agcaatcatt gttgcgccta
    21661 atgcaggtcc ttcctccata cttaaacaat agaccgttgc atcgaaaata tcagcttgga
    21721 tttgtagcca tgcaagattt ttcgcgcctc ctcccaccga aacaatacgt gtaaatctgc
    21781 gacccgctag ttctttacgt ttcaatccca ccattgacaa atgctcatta aaaatttaga
    21841 atattcacaa gtttaacgac aattatgaaa atttatgtcg tgctaaagga gttttgttat
    21901 tggatgaaaa gttagatata aaaactgcga tcaaagacac gttacctacc gttttcggtt
    21961 atatcggtat tggacttgca tttggtatcg ttgggaaagc tgccggattt catccactag
    22021 tcgtcacgtt gatgtccctg ctggtctatg ctggttctgc ccaatttatc acagtcagca
    22081 tgcttgctag tcacagccca ttgctttcca tcgttttctc gacctttcta gtcaattccc
    22141 gaatgattct gatgagtatg acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa
    22201 atctgctgat tggcacattg ctgacagatg aaagtttcgc actgggaatg aataaattga
    22261 actatactgg acaaaagtta aactttcgtt ggatgaatac agctaattgg atttcttatt
    22321 tgacgtgggt gggttcttct cttgttggcg cacttttagg gaattttatt actgatccga
    22381 aaaagtttgg tttggatttc gccatcgtcg ctatgtttat cgggttactt tatcttcaag
    22441 tcatttctga tagaaacacc tctaaacgat tacagttgat cctgattggt ctcactttga
    22501 ttctggtata tgtcggactg atttttatcc caagcaactt agtgattgta gttgtaacac
    22561 tgattgggtg cggattgggg gtgtggatca aacatgcctt cttttgagtt tacattgctg
    22621 actattttag gttgtacttt agcaacttgg ctttctaggg tcctaccatt tgtcttactg
    22681 aaaaaattcg atttaccgca accgttgctt gagtatttaa gttttgtccc gatcgtcatt
    22741 atgtctgctt tatggttcag cagtctattt acccaaaata tcggacatct gccgcagatc
    22801 aatctcgaaa atgctttggc ttcagttcct acacttttag ctgctattct ttcaaaaagc
    22861 ttattggtga tcgtcttggc aggaattctt tccctttcgt tgattcgctt attataaata
    22921 aaaaaacaag agcttatggc aaattactgt catgcgctct tgtttttatc aaagtcaaat
    22981 aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg accactaaca acgacttttc
    23041 ctaacatatg tcctgtttct acgtctttgg ttgcttgttt caagttcttc gcattcatcc
    23101 catctgaata gaccttgctc aatgtactgc ggataatccc ttcatttgct aaagcagcaa
    23161 tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca
    23221 tatactccca gtcgaagctg atagattggt ttttccaaac acctatatct aacggctctt
    23281 tgatccctac gatcgttccc acatgtccaa atggtcggac aaggtgcttg atttgatcaa
    23341 agtagcgggt aatatcgaat aatacagcga catactctac ttgatcgatt cctaattcac
    23401 gaaaactttt acttaagtcg tgatgataat cgattggata gtctacacct gtttttttca
    23461 gccattcaaa attcttcgga cttgcagtcg cgtagacttc taggccagcc caatgcgcta
    23521 gttgattcaa aatcgaaccg acgcccccgg atccgttgat cactaagatt tttttcccgc
    23581 gattggcatt ttcttctgga gttagaccaa atttttcaaa taacaattca taagcggtca
    23641 atgaggtcaa cggcaaggct gcagcttctt catctgaaag attttttggt gcaagagcga
    23701 caatacgttc gtcaactagt tggtattctt gattgcttcc tgctcttgtt gttgtacctg
    23761 cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc gtcacctaca gcaacaactt
    23821 tcccgactcc atcaaaacct aagatacgta aagcatttct tatcccgtta tcttggcgaa
    23881 gtttcgtatc gacaggatta acagatactg cgttgacttt taccagaaga tcccgtgaac
    23941 tgggtacagg tatctgttct ttttcatcaa taaagctttt tggatcatcg atcggcaacc
    24001 cttcataaaa accgactacc ttcattaatt ccttttctga catacgaatt cctccttagc
    24061 ttcatcaagt tggagcatcc atacagtgtt catttcaata tatatagtat gctatttgtt
    24121 ccaccttttt tattcatcat aattattctc ttacttaaag taaactattt gcactttttt
    24181 ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttataaacg gtgttctcct
    24241 accaactctt cggtcacaag tcaaaatatt catcaaacat gagaagctgt tttttgaaat
    24301 tgctccttgt gactcagagt taaacggcag gctcacaacc tctgatataa acggtgttca
    24361 atcaactgac cctcggtatt agctcaaaaa acgacaaaat atgaagagct atttttgttt
    24421 ggctgttctt attacttgat acaggacagc tttttcacga cctctaattc acggtgttca
    24481 agcaagaaat cattgttcac tgaaaagcgt tggatgttct ttttcaaagg tctctaagat
    24541 ctctcgtact tcatcagtag atttcgtatt catcaactga actcgcaagt catttgctcc
    24601 tggaaatcct ttgatataga ttttaaagaa acgatgtagt cctgtgatgg aacgcgggat
    24661 gatttctgaa taatgatctt gcagatctag ttgcagacga agtagtccga tcagttcttt
    24721 ttctgtatgt tccctcgggt ctttttcaaa tgcataagga ttcttgaaaa tccctcggcc
    24781 aatcatgatg ccatcaacgc catatttctc ggccaattct tctcctgtct ggcgatcggg
    24841 gatatctcca ttgatcgtga ttagtgtttg tggagcgatt tggtcacgta aagcaatgat
    24901 tttaggaatg acttcccaat gtgcatctac tttactcatc tctttacgcg tacgcaaatg
    24961 gacagaaaga ttggctatat cttgatgaag taggtgactg atccattctt ccatctcaga
    25021 catttccgta tagccgatac gcgtcttgac gctgacaggc aaaccgccag ctttagctgc
    25081 ttcaatcaat tgtgccgcta cttctggacg aaggatcaag ccactgcctt ttccacgttc
    25141 tgctacatta gggactgggc agcccatgtt gatatcgatt ccttggaagc ccatttctgc
    25201 catagcaatg ctcatttctc taaaaaattc tggcttgtct ccccaaatat gggcaaccat
    25261 cggctgttca tcttctgtaa atgttaatct tcctcgtaca ctgtcttttc catctggatg
    25321 acagaaactg tctgagtttg taaactcagt aaagaagaca tccggtgctg ctgcatgctt
    25381 tacgacatgg cgaaatacaa catctgttac atcttccatt ggtgctaaaa caaaaaaagg
    25441 ctttggcagc tctttccaaa aattagtagt catttatttg ttactctcca ttctatcaag
    25501 cagtgaagtc gcaatctttc tcaaaaacat tgtcattata ctaggaaaca aaaaaagaag
    25561 caaagtgaac agcttaaaag atgtttctga ttgagcaaaa aaaagactgt gacaaaaatc
    25621 ttatcacagt ccattttccg gaataaatgg tgttcaaaag cttgcatttg cggtaataag
    25681 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
    25741 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
    25801 cgctcaaaaa acaacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
    25861 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
    25921 aataccaagt aacggcgtac aaaacaacga tgaataacac aagtacgatc atcgatgttg
    25981 ctactggcac atcgatcgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
    26041 tcggattgac ttttgaaaca gcttgccaaa agggcggtaa catagagatg gcatagaaaa
    26101 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcatcat
    26161 aagactgagc aaaaatcccg ttgagcagtc ctgctaatga aaacaagatt gccgtcatca
    26221 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
    26281 tgatcgttac gagagagccg acgagaatac tgcgacccac accaccaatc acaaagcccc
    26341 aaataatcac atgcgtagga acaggtgcta ccaagatttc ttcgatattt ttctgaaatt
    26401 tttgagagaa gaaggacgaa gaaacattgg catacgaact agtgatagca gacatcataa
    26461 tcaaaccagg aacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgcccac
    26521 cgatcatttt tccgaaaata acaaaataca aggatgtcgt gatcactggc ggaaccagtg
    26581 tttggaccca tatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
    26641 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaag
    26701 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
    26761 gtaagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
    26821 tgctcatctt caaatgtaca agcatatcct tcaatcacag gtttggtatc atatggcgct
    26881 aaatcaaaga taaatgtttc aaattgcagt tttgacagaa gctctttcat acttgtattc
    26941 tcgatcagtt cacccgattg gatgattccg atgttgcgac aaagcatttc agcttcttct
    27001 agataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg tagaaactcc
    27061 cacatctcgc gtctcaactc gatgtcaact cctgctgttg gttcatccaa gatcagcaaa
    27121 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
    27181 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
    27241 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
    27301 tggaccgttt caaaagggtt aaaattgaac tcttgtggaa ccaatccaat ctgttgcttt
    27361 gctcgaacta gatccttgtc cagatcataa ccgaagacat tcacttttcc agatgtttta
    27421 ttgactaaag aagtaataat cccgatcgtt gtagacttcc cagctccatt tggccctaat
    27481 agcgcataga aatctccttc tttcaccgaa agatcgattc cccgcaatgc ttcgacccca
    27541 cttccataga tctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
    27601 ccttatagac atgattctta attgtatcat ggaatacagc agatgaaaaa acaacttggc
    27661 ttccacacaa aaacacacct gctaacaaag aggagatgtg ttttctagtt catgatcatc
    27721 ttcgatagaa taactatcta aacaactctg gaacttttaa aatcataaaa gtaccgacag
    27781 caactactac gatgacatat agaagatcct tataactgag gttcttgtct tttttattca
    27841 tgttaagctt tcctccgtcc ttttatcgaa agcttttttc ttttatttta accgaaataa
    27901 ataaaatgga aatagttatt ttcaatttct gttttcgctg tttgttttac ggagaacttt
    27961 gccatggaac gtgcgtctcg tcgctgatgc taagtttttt cgataaaagc tgctagaagg
    28021 aagacagcgt ggtttatttt gtatcgtctt ttgctaaatc ttccaagatc atctggacag
    28081 cagactcgtt cccattgaat tctgccactt ttttggcatc aatgtacttc ttttgataat
    28141 cgtctttttg gacccacgca tcatgcttcg tttgataaag ttaatcaaac ggataccact
    28201 gacgctgttc atcgaacaac aaactttctt tttgggcctg ttcaactatg gatttaggat
    28261 aattcaaaac tttcagcaag gcaatcgcat ttcgggtcct tgaaggtcct tcttttaatt
    28321 tgaaatcaaa actgattcct tttccttctg ttacttgttc ttcaaagtgg acattctcac
    28381 aataattctt taaaatctct gtcaattcga tatcatgtgt ggcgacaaat gctaaagatg
    28441 gatactccgc aagccaccga acgactgaag cggaagaagc aatccgttcg atcgtatttg
    28501 ttccttttaa aatctcatct acgaagcagt agcagcgttc tttggaagcc acttggtcca
    28561 gcaaacgttt gatcgatttg atttctgaaa caaaataact gtcgccttca aataaatcat
    28621 cttcgattgc catggaagtc aacacatgtc caggttgcag ggtaaactgt tctgcaatcg
    28681 ctgtttggat cgtctgtgct agaatacagc tgatggctat acttttgaca tacgtagatt
    28741 ttcctgatgc attggaaccc gtgactaatg tattcttctg ccagttgact ggattaacta
    28801 ccgcttcttt taacaatgga tgatagatat ctttcgctgt cacaccgccc tcagtaaact
    28861 ctggtcggca agtgatcggc atataggtgc gataattcaa aacggctgct gcaacttcca
    28921 attctcccag caattcccac agcttgatgg cttcagttga atgcttttct aatttagtga
    28981 gaacgaaatg ataggagata aatggcagca tgaacatgat gttgacatat tcaaaaatca
    29041 attctgcttc actcccgctc ttcattcgaa atgaaaaacc taatttaggg atgtctctta
    29101 atggtttagt ttgctgtttc agctcttttt gaagtggtgt cgtcattttt gctaactgat
    29161 tggcggctga aatcgttgaa accaaataac tcatgctatt cagctctgtt tctagtgttt
    29221 gtttcttcac tagataatag accgtattga atactaagcc accgattgct agatagattc
    29281 ctagtacttg ccctaaaaga agcaaaccta cacccaccag cggaagcaag ccaagaaaaa
    29341 taaataaacc tacgcgtcca atctttttac tttttccatc cgctagatat tgttttgcaa
    29401 aattatgatc tttttttcct aaacgagcga attgatattg gattctttct ctcagttgag
    29461 gattttcctg ataaaaagca attagttttt ctaactgata atcttctcca aaatcaaagc
    29521 tacgtaaacg ctgatacaac gcttctgaac ctacactaga ataggtactg ttgatccctt
    29581 caaatacttc gaacatatcc aagtcatacc aagtaagatc gtcgatttca ctatcccatg
    29641 aacggaattt tttctccgtg agccacgcat ccttcaaact ttcttcttta tcaaatctcg
    29701 gttgataagg aatttttccc caattactac gaactgtttc ctttactttc aaacgtgaaa
    29761 aaatgtctaa aacaactaat aaaataacaa ctgcaataat aattaaaacg ataaattctc
    29821 tttggcccat atccctctcc gttccttgat ttgttagttt tagtatagca ctgttgcgct
    29881 atttgaaaag ggggtacaaa aataactgtc ttcgttacct aattcgtatc aatcacacca
    29941 aaataaaaac accttccaaa aaagaagatg tctttaaaac gagatacaat tttttgtcat
    30001 tcacttcagg taaattagag ccaattaaac cctccgcaac ttgacggaga tggagaagat
    30061 gcccccgtct aatctagcgg tcatctctcc tcccattcct tccaccaatt tttttgacag
    30121 gtaaagcccg attccgctag attccgtatt tgtgcggctg gtattttcgg tataaaaacg
    30181 ctggaagact ttttcaacag aggtttgcgc tcctggtttg ataggattgg ccactgttaa
    30241 tcgaatgaat gaatcctctt ttgaaaatgt cacctttgct tgtccatctg cataacgcaa
    30301 taaattctgt atgatatttt gcagcactcg cagcaacatg agcggatctg ctaatacagt
    30361 tgtatttatc gagtgttcag cgaacgctac ctcgatattt ttctcagaaa attgttcata
    30421 aaaagagaaa aatacttctt ccacctcttt agaaaaagaa agcgtatgga gatccatttc
    30481 tttttgcttc gtctctattc gagcaagatc ataaaactga tccgtcatac tgagtaatct
    30541 tgcaacagat tgtttgatga tcgttaaata cttctcccgt ttctccgtat cctctacctg
    30601 ttccagtaat tgtacataac cgttaatagc agtcaaagga gttctgagat catgagaaag
    30661 gccgacgatc gatagtctga tgttttcttc catttcttga aatgcttgtc ctttttgttc
    30721 attttcatcc acccatgtat tcagtgcatc tactaggtcc attagattct tttctcgaaa
    30781 ttcgactgaa agacgaccac catattcttg tcgttcagaa agagtggcga ttttcttagt
    30841 taatctcttg atttcccttt gcaggaagaa aataaaactt cccataaaga taacaacgag
    30901 aagcaacagc cacttaatca aatctaaacc ccactcccca caccgtatga atatacacag
    30961 catgtgcctt tgctagtttc cctcgaagat tggaaatatg catattcact gttttctcac
    31021 tatcaaaata tggttcttgc caaactgttt cataaagatt tgctcgcgaa aatacttttt
    31081 tcggatgttc taaaaaaagc ttcagcagat cgaattctct tcctgtcaaa tggacagcca
    31141 caccattcac atttacatcc ctcgactctt tgtccagtaa gatttccttg taatagatgc
    31201 gttgcgacag atcttttact tggacagaag tctgatgacg aagttggatg ccgattcgta
    31261 acaacagttc ttcgatatca aatggtttcg tcacatagtc atccgctcct gctgccaaca
    31321 aggaaacttt atctctttga tcgctttttg ctgaaagaac gatgatcgga acattcgatg
    31381 tctgtcggat ttctgctaac aatgcttcac ctgaaagccc tggaagcatg agatctagaa
    31441 ggatcaaatc gacaggatat gtgcttaaca ataattttcc ttccgtacct gaataagcat
    31501 caagaagttt gtatctctgt tcaagaacct cctccagtaa agaatgaaca gcttcatcgt
    31561 cttcgataat caaaatagtt tccagcagga acacctactt aatgtcagat ttttggaaaa
    31621 taaacattcc tagagctgat gataccacga ttgttactag actgaccaga atcattttcg
    31681 gtgcttgcga ataagttgct tgttgcgcaa agtccgtata catgagccac gtttccgtaa
    31741 accactccgt tagaaattcc aagcctttga tttttgcgaa aacaccggga acgaaggccg
    31801 ttcccatgat ataaagaaaa gaaagaagca aactgacact ggtacttcta gtgatgaatg
    31861 tcaaagccac tggaacagat aatgtcgcta atatcagcag aagcaataca ggaaatactt
    31921 ttagaacata atttccatag tttttgattc cctcaacttc accaaatccg aaagcaagtg
    31981 tgtaagcaat catggctagc cccgtaatca aaaccacgcc aagtgttgtg atcaaaagcg
    32041 taacgagaag tttcgacaca taataagtca ttcgattccg tcctaaagat aacgtattgc
    32101 gaatcgtacc gttttgaaat tcttccccaa tataaaagca cgcaaatgct gcgaaagcca
    32161 gaagaatcca tgcagccatt ttttccacca ttaaaatccc tgttgcaccg ttgatgactg
    32221 tatccgttgc ttggtttaat tcattgccgt attttgtatg catataccat tcgttccaag
    32281 aataaaaaag cggaagtacg accatcagtc ctagtgagac ccacatttta cggctcttcc
    32341 atacttttat tttttcagca gttaatagat taaacatgtg gattgcctcc agttaattgt
    32401 aagaaataat cttccagttt ttgcccaact aggtggatac catccacagg aacttttgcc
    32461 tctaccaatg cttgattgat ggctgctact tggtcaattc cttcaaaaag ctggataaca
    32521 cggctagatt ggacaaaata atccctataa ccaagagagt cgagaaccgt tactgcttgt
    32581 tccgtcgccg aagtgtcaat ttttataaat tgacgagatt cttgtgccaa ttcttctttc
    32641 gataattctt tgatcaagtg tccttcatgc aaaataccgt agtgagtggc gatctgtgac
    32701 aattcatcta gcaagtggct agaaagcaag acggtgatgc cccgttcttt taccaaacgt
    32761 tggatgattt ctcgcatttc tgccatccct gctggatcca tcccattcgt cggttcatcc
    32821 aaaatcaaaa attctggatt cgtgatcagt gcatttgcaa tagctaaacg ctggcgcata
    32881 cctaaagaga aattctttgc tttctttttc cctgtatttt ccaatcgcat cattttcagc
    32941 aggtcttcga tttctctatc cgacacaccg ccgttcgctg cttggatacg taggttatct
    33001 cttgcagtta attctggata aagagcaggg gtttcaatcg attgacccat acgtcgtctt
    33061 gctgcctgca aatccgtcgt tccaaataat tcgatcgaac cttcatcaat cgagatcaaa
    33121 cccataatag ttctcataaa ggtagactta cccgctccat tctccccaat caaaccatag
    33181 atcattccac gtttgatttc gatcgatact ttatctaatg ctttggcagc accgtatttt
    33241 ttggttatac cagttgcttt caagaccgtt tcatttccaa aatccatact tatccttctt
    33301 tcttattgat aagtgaagta tagttgacgg gtataaagaa agcagccaga aatgtataaa
    33361 gaaagtgtaa attttccaat tgaatttcaa gaaaaatcag actgtctcgc agacgtggtt
    33421 gagttgtctg ccaatcaaag agcgcaccaa acagtgctaa tccaagtgga ctggccacca
    33481 tggtaatcgt tgttatcgtt gacatcagac gtccttgtat atgcgttgga acttctcgct
    33541 gaagccagac catataaaaa gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa
    33601 aataatacat gccgtttggg atttacaatt agctaccaaa taagaggtta tgacaaaaga
    33661 cttttcacaa tcccttcttc cgaataaacg gtgttctcct gccaactcct cggtcacaag
    33721 tcacaatatt tatcaaacat gagaagcaat ttttgtaaat tccttcttat ttcttgaagc
    33781 tgaccacttc tgtcacaacc tcagaagcgg ataaataata ttgtttgatg atactttatc
    33841 tttttcccgt ttgtttatag atcgaatgat attatagacc aagaatccta cctattaaca
    33901 tagctaaaaa cacaaaaaag gcactttcca aattgtggaa agtgccagaa aagttaggat
    33961 taaggtaact gcgctcagca ttgcttcgcc cagtacgatt ctcgtaactc gtgaactcga
    34021 acgataatcg caattaacgt tttgagaact gtgaagcttt acgggctttc ttaagacctg
    34081 gttaaacaaa cttttgtttt ataatgtttt acagagaaag aaatcgtgta aaatcagcat
    34141 ttaatgattc atataattct acttagttaa ataaagttat atcctgtcat tacctcaaat
    34201 cattaccaca acttggcgag ttggctgatt gaagaatatt ctgtgccaaa taaaaaaaat
    34261 gatgccaaaa ataagtacac tactcaatac ttattttcaa catcattaat cctaaaatag
    34321 cttcgctttt acccgttact atctaccctt tcggaagggg tatttcatct gactaaagaa
    34381 tagcatccaa gataccaata gtcaagtaat taatttacca tactccatta caattttttc
    34441 aaaaaagtgg aaaacttcta caagttcatg attcttttcg tacattttac cattaacttt
    34501 tatgcgatca agtaaatcag atagagcctc aaagtttgtt gttttcaact tttgtgtttg
    34561 aacatatttt tcgtgaagat atatagtagc cacaatatca ttcattttca tattggtgag
    34621 tgctctggcg attagttcag aatcaagacc cgcattggtt gcaaactgtg ttaattgaat
    34681 aacaggcctt cttttcttca agttgctgtc agctctacca aattgataag gttcaacaat
    34741 attataaata agaggtctac tatgtgcaga ggcatttctg acattcgtaa catattttaa
    34801 taatttttga gctgttttta atcctagata tccaaaatgt tcttcttcaa aatagaaggt
    34861 gataaatctc tctaaatttc ctagggtcat aacttctaat aaagcccaaa ttgaaatctt
    34921 atcatgatga tgattataaa aatcaaaatc atatccgaac ttccctttca ctttatctag
    34981 aatcttttca gaagcttgtg tatattcaga caagatttct tcgtttgttt tagtattacc
    35041 tttttttctt tgactatcaa taaatttatt ttgcatatac atatcgtaat cttcaacaat
    35101 tttatatcca tcttcggtta atgaattagt gattgaactt attatcaaag ttttcaaaga
    35161 atgttcaagt tcaaaaatca ttctactcaa aatcattctt aaatacatat caatctttgc
    35221 taaatcagct aattttacaa atgataaatc tatatacttt ccatttatat ctttttcaaa
    35281 attcttacgg tatgcggtta ccttaaaata atacgttcga tataataaaa tatcctgaac
    35341 aatactttca tccgattcag aaatttctat tcctttaaat ctagcatatt taatttgatc
    35401 cgtaattgaa aaaatttcct tgtttttagc ttttttacaa agatctttaa tttcttctga
    35461 taattcttca gagacttgat taactctctc ttcttcttct aactgcaact ttaatttagc
    35521 caatttgtcg cttttaataa aaacacacct cctttatctc tttattttta tactatcttc
    35581 ccaacatctc cataaacttc cgcaattctt cttgcgcttc atctgttgtc ccgtgcaatt
    35641 cacctagcat ttcaaataat ttatctgtcg atttttctat ctcttgcttc acatctgcta
    35701 attcttgtgc aaggtgtggt aatggagtca cttcttcttc ctcaaacgtg tctacatatc
    35761 ttggaatgtt taggttataa tcattctctt taatttcgtc taaagttgct acatgtgcat
    35821 atttttcaac atcttcacgt ttttcatagg tttcaataat tttgtcaatg ttttctggtg
    35881 ataatttatt ctgatttttc cctttgacaa attcactaga agcatcaata aatagaatat
    35941 cttttgttct cctagattct cttcctttga ataccaacac agcagttgga atacttgtcc
    36001 cataaaatag atttgccggc aatccgataa cagtgtctaa caagttatta tcaatgatat
    36061 ttttccggat tctcccttca gaagctcctc ggaatagtac accatgaggc aatacaatcg
    36121 ccatcgttcc agttttatct aagtgataca ggccatgtag cacaaacgca tagtctgctt
    36181 tagaagcggg tgctacgcca taacctttaa accgtgtgtc tttttctcga tccacattct
    36241 ttatatccca cttagcagaa tatggcggat tagccactac agcatcaaat tttaatggaa
    36301 tttgcatacc atctttttct gcgaatggcc agtctgcatc aagggtatca ccacgtttca
    36361 aattcatatt tcgataatta ataccatgca tcatcagatt catacgagct aagttatagg
    36421 ttgtcgtatt caactcttgc ccatagaaat caacactgcc ttgcttattg ccatcgggta
    36481 gttcattttt cactgtcagc agcaacgagc ctgatcccat tgctggatca taaactcgaa
    36541 attgagtatc atccgctttc gcatctttgg ttacaatctt tgctagtact tggcttactt
    36601 catgtggggt ataaaattct ccaccttttt tccctgcatt ggcagcaaat tgaccaatta
    36661 agtattcata cacatcaccc aggatatcgt gaccagaatc atctttaaag gtaaaatcgt
    36721 tcaccatcaa aacaacatca ttcaaggctt ttgctcgttc attcgtattt gatcctaacc
    36781 gagtatctcc aagattaatg tccgaaaaaa cattggcaaa atcatcttcg gcaactggat
    36841 tccgtttcgc attcaaattg aacgaatcaa gcatatcctg aaagtcactc gctttgattt
    36901 ggtggttttc aatctttgat actaactgtt cccaagtata ttccggagca atcgcataac
    36961 cgattcctcg actgatttct tccaagtaat cttctttttc ttcagagtca gtgacttcgt
    37021 agtactcgtc taacccattt ttttctaaat actcgtcttg attttctgat aagtagcggt
    37081 agaacataaa tggtaaaata taatttttat actcacttgc atccattgtt ccacgtaatt
    37141 tattggccat tgcccataat tttgctgtaa tctcacttgc attactcatt aattttctct
    37201 ccttttagac aaacattttt tgtagcaatg tttttttcat ttcttttagt gaatccaact
    37261 cacgctgatg aagggtgatg gtggtgtcga gacttttaaa gaagtccgca attttttctt
    37321 gctcgttatt attttttgga atcatgagtt cagtttcaaa aaaatcagat gcagcaatat
    37381 ttaaaagccc atgattccta gctccctcag cagcatgctt agaaacctct ttataccaac
    37441 gatctgtatc ataatatgac gctaaaaaat ctgaatttat gtcgaccggt ctaaaacaaa
    37501 tatacaatgt tgataataca cccatgtcat acttatctag tcgcttaaca gcaccccaag
    37561 gatagccatt ggagtagctt ttattatagg caaactcacc tttcttcagc agataatatc
    37621 cgctaacatc cttactggca atttgcttat taaaaaaagt attctgatca actaatccaa
    37681 attgtgctga tatagtcagt ggaagtgttg attcaagctt tttgttcttt ctggtcaccc
    37741 tagtggcaac ttcccccaac ttacgctgtt cccaagcgtc agtaaatcca gcaaaacgaa
    37801 tttctggttt atcttcacct gttttaggaa acattttttt taaataagtt tgttttgttt
    37861 gctctaacag atcaagctca cgctgatgaa gggtgatggt cgtgtcaagg ctctcaaaaa
    37921 aggtagaaat ttgcttttgt tcttcaatac taggaaccat tactttaact gattttagcg
    37981 ttggaaattt caaattccat gtatcagatg ttaagccttg tgagtttctc ttgaacgttt
    38041 gaatcatgtc atatcgcttg aaatcatacg caaagaactt cgagtcaata tctttttttg
    38101 gaatgataac tgtgtatgca gggcttagaa tgccattata aggggaatat ccgcttgcac
    38161 cttgccacat tctcattgag ttgtatgcaa tatcaccaac ttcaactttc ttgtaatttg
    38221 acttgtcatc actggaatta tcttttctat caagttcgct tgcctttaca actcctgaat
    38281 taatagtgac cgagataagc tctccttcgc cactacgttc tgaccgttca gagaacacct
    38341 ccccaacctt acgctcttcc caatctccct cgtatccgtc aaatcgaatt tcaggttctt
    38401 ttgttatttc attgctcatt ttaactctcc ttcgatcttc gttatctcct tatcaaaatc
    38461 agaaacgata tgctgatgtt tattgaactc cttgtactca tcaaccgctt ttctcttagc
    38521 tgctttactg ctaatttttc cgtttccatc aagaatttga tagcgattaa atgttagaaa
    38581 ttcatctatg cttttggcaa aatcttccat acctaaaagt gtttcatctt ccaataatcg
    38641 ctcaacataa tcaaaataag aagatacgtt ccgttcaaga ctgcgaattt gtttttctga
    38701 taaataattt ttagcaactg atacatctga ctgtaggatt cttccatctg gagagttttt
    38761 ccaagtcgtc agtcccatgt tctcttttgt tttatctgca ccattaaaaa caatctccgc
    38821 agctgttttt ccagtaatag catagtgaaa tttattttgt acagtggcat aaaactgctt
    38881 cgtaatttca gagtttttgt cataatcata agagatttct gcaaatacat cagtaatttg
    38941 ttgccaaatt cttcgttcac tggcacgaat tgagcgaacc gtttctaata actctcggaa
    39001 gtaatccttt ccaaaagcat tctcaccttg tttcattcgc tctacgtcaa ttttgaagcc
    39061 cttttgaata tagtctttca gaatatctgt tgcccagatt cgaaattttg ttgctttttg
    39121 agaattaact cggtaaccaa tagaaataat cgcatcgaga ttataatatt tgaccttatt
    39181 tgtttgtgtt tttccatcta ttgcgccatg ttgagtggta ttttccaaaa tggaaacaac
    39241 cacttcttca tctaattcac cagactcaaa tatatttttt aaatgcttgc taattgctgg
    39301 taccccaaca tcaaaaagtt gtgaaatcgt cttttgcgtc gcccaaattg tctcttctcg
    39361 aataagcact tcgactttat cactatcttc ataaattaaa aactgaattt cattcaatag
    39421 aacttactcc cctttcttag tctcgtccgc catctgatag aaagctttcc gaaattcaat
    39481 tcgatactta acccatgaaa gattcgccac ttccgctgct gcgatttctt tataatctgt
    39541 tcgtgcatcg ttcatcattg ctgttagttc accttgtttg tccatatctt cttgaccaac
    39601 tcgatgccgt gaaattaatt tggctaaatc ttttggcttc acactattat ccaatcccca
    39661 tgtgcgaaca aattctgtat atagtttgat attgctatct ttttgagatt gttccatcgc
    39721 ttgattcatt tcttcaacat ctctaggagc cggatactta tcaaagatga attccttcgt
    39781 aaagatcttg gaaacaaaat tgcgaacttt cgtaatctcc ttatcgctat ccgattttgc
    39841 aatttcaaca tcgatctcat tttttgtcag ctcagcttga tccattttat cttcatgaac
    39901 ctcatctgcc attcgtgcga tcaattcaat cagataatca taattgatcg tcacatcgtg
    39961 aatatgcgtc atctctaaat tgacttgcga tatatcgata ttttcgattt gggctcggcg
    40021 ttcacgcaac tcgttagcaa gtaccgttgt cagcatgacc tcttgatcct ctgtaattcc
    40081 taagcggtca tagaacagtt ccggttcatc ataaacagaa acaggatttt tttcatcgtc
    40141 ctccgtgaat tgcttcagtt gatttagtaa tcgattgtac tcctgtaatc cttcaaatac
    40201 ttcctcttgc gctttttcac ttggaggtaa ttgaataaaa ttatctgtta gttcagctaa
    40261 tttctgaatc acttctttgg tttctgattc gattttacta aatgatttcg ataaaattcc
    40321 cgactcttca ttgtcttttt ttaattcatc tagcgatagt tgctctgctg ctgaatctcg
    40381 atttgaataa atagcaaaag acttattcat ttcgtattca ttttggatgg gccaacgata
    40441 attaacaata tttccttgtg gctttgcatc cctatcatgg attctattcg tacgtgaata
    40501 tgcttgaatt agattgccac cttttaacgt acgatcaatg tacaatgtat taagctccgg
    40561 tgcatcaaaa cctgttaata gttgatccac gacaatgact agatccaaat aatttccatc
    40621 gtcagctgtt ttgttcaaac gtctagctaa atcttctgta tatgatttaa ctgtcgtcat
    40681 atcaaaagca gtaccaaaca atctattata agattgaatt gctcggtgaa gattttcatt
    40741 tgtttttagc tgatgactac tatttgatgt atccatcgaa aaactaatgg caacttttat
    40801 ctgatcctca attgaccgtt tattattttc ttccataaat ttatcaaaat attccattgc
    40861 tcgaggagta ctagcacgat ttccccctac atgtactgta aataacgcat tgtatttccg
    40921 attattagag cgttctttcc agttatcaaa aatatcttca acaacgagtt ctacatgctc
    40981 gggactatga tcatacacac tgccacgaag attgtcgtca atatcttctt ctgtaggatc
    41041 tgctggtgct tcaatcgtct ccttaaactc cactttgaat cccaaaacgt ttttatcagc
    41101 gatagcttct ttaatcgtat aagcatgtaa taagtcacca aaaatttctc gcgtttctgg
    41161 aaacttaggt gtacctgtat aaccaaccca agcagagtta ggcaaggctt ttcgaataga
    41221 ttctagcatc ccttcatttt tagtcccatc acctgttgaa cgatgtgctt catcaacaat
    41281 aaataataca ttctcagtta atggttgaaa gctgtctctt gttacatatc gagacatttt
    41341 ttgaatactt gttactatga tgtttttatc actttttttg gtaagtttac ggtgcaagtc
    41401 agaaatattg gcagtatcac ttacaacacc agttttccct tcaaatccag cgattggatc
    41461 atatgctcta taggcatctg ctgtctggtt tgtcaatgca attcggtcta ccaagaaaat
    41521 aaccttgtct acatttgata agcgactcgc taaccatgcc gttttaaaac ttgtaatcgt
    41581 ttttcctgaa cctgtcgtat gccaaatata gcctagcttg ccctcgtcat gcttaaaatc
    41641 aaatttccta actttatcaa gtacccgttt tgttgcatag acttgatacg gacgcatgac
    41701 tttgatactt tctttgttct ttgtaccatc caacaccata tatcgagtag ataaatcatg
    41761 ggccatggga atagacaata ctttatctgc aaacagtttc catgaacgta ctggatgagc
    41821 tgtttcttca tcttgccaat tgaaagcaaa tgcacgattg aaattttgta atgtagtatt
    41881 tgccatgtaa cgaatatcaa acggggtcat cgcaatgagt atctgtaagg tagaaaaaat
    41941 cccactaaat tgtttctcag caatatattg ttccatttga tttaaagctt ctgtcgcatt
    42001 atgtagcgcc tttttcaact caatttgaat aatcggtaac ccattaatca aaagcgtaat
    42061 atcaaaccga cgattgggct taccatcaac aacttttgga cgttgaattt gattaacgac
    42121 ttgatagact gtatttccgc cgccaacttg ggcttgatca aaaacggtca aaaaaacatg
    42181 ctttccgtta tcaagatcaa cttcaatttc tgatacacca tttacaccat ataaaaattg
    42241 tccagcttga taaggcgtat taatattggt aataattttt ttcacttgat tgaactctgt
    42301 tgcagataac ggttcatcta gccgagcttg attattttgt tctagaattt ttttgaagtt
    42361 gttccataat tgttctgttg ttttaatttc agctttatat tcccattgtt ttacgccacc
    42421 gattcttgtt agataatcca caacttcttg ctcgaacctt aattcagatt gactttttat
    42481 accgaattgc tttgtcataa tctattcctc caaacttttt tctaacatag aatttgtaaa
    42541 ttgctctaac aagcgagtat atatacttaa cttactttgt aatttcaatg attcagtgta
    42601 aatcccccct atttttcttt gttcttctat aagaggtagc ggaataacaa gttgttccag
    42661 agactgtttt gttaacctta aaactggacc tgttccttga agacctcgat ctttttgacg
    42721 ttgaatatct ttatgattgt taaataagta aagaaaatag cgcttgtcta gcaaatcact
    42781 tttaaactct acttttgtaa aatttagaga cagaacttta ccaacattct gtccacttac
    42841 cattgttgcc cgttgaagtg aattactaat aacaacatcg cgtttatcta aagatacttc
    42901 ttgatcaaat aaatagttta ctctttcctc ttgagtcgct tcattataat acttatgatc
    42961 ttcttcaaat gaagcttgat cgtaataatc aatttcttga ttgccaaact gtttttcagc
    43021 tcttgattga ttaattcctt gaacaaaggt tacaaactca gaaagctttc tcatttccat
    43081 atatacttcc tcctaaaata caattcaggt ctcatttaaa cgatttatcg aaataatcat
    43141 atatttcttt ttcgtttctg cgtttttata ataaacctta tacagtaatt cgtcaatgga
    43201 aataatttta tattacaaaa aatccgcaaa aaatgcggat tcctttaaat atttaatctt
    43261 tattctagtg tagtacaagc ctatacccta acgttacata tctcattcat tattatacca
    43321 gtatttcttc atgacacata agggtgtaac ataataattt taaacgaaat gactacaagt
    43381 agtcattgaa gtaagtcttc ataagatacc tgtaggatat ccctaattgc tctcaattga
    43441 tctaacttaa tgtgttgccg gccaccttca attttaacca atgtttccct agttatagca
    43501 attttatgaa gctgtaggtc tctaatcagc tcggtttgac caattccttt ttctaatcgg
    43561 attcgtctaa tattgctacc gattacattt tctttatcaa taattttttc ctctttcaag
    43621 aatagcacac cttttggact aaaattagtc ctttttctta ttaatcttaa agattcggta
    43681 tgctacaatg ggactagtat tagtccacat tcaattttaa ggaggaattt ttttgaagaa
    43741 atttgcaatc attctaacct gcgctttact catcggaact ataagcgggt gtaccacttc
    43801 tgaaacaagt tctacaagta cggaaactag tacaacaacc tcagaaaaga attacccaat
    43861 acattttttt ccggttccag atggatttga agcttattat catgataaaa aactagatgt
    43921 ccctttatca gaaactgaat ctactgaaaa gattttgcca attgaaattg aaagaaatca
    43981 agacgatata aaactaaaag ctgatcttcc cttactgaac agcacacaaa ttgatagaca
    44041 tgaaaatatt aaagttaata ttgaaaaagg agcgttagca aaactttata ctgaaaaagt
    44101 tgaacaattt tttaagtctg gtgccaataa agacttttca aaaataagcg attattcgga
    44161 gaactttttt aatgaaaccc aagatgaaat catgaactca acttttatgg ctgaagatat
    44221 gtacaatatg acattaaaaa aaataaaagc ttacgaccat ccgttaaatc tgacactaaa
    44281 taatggactg ctttcttttg tattagcagg ttacgttgac tatgaatatt caccagatta
    44341 tttggagaaa cctcttcaac acgagaacaa tgatattaat tttagatttg tctacgagtc
    44401 aaaaagtaaa acatggttag ttgatcaatt aagcacgata aacacaagct tccatgaatt
    44461 acctaaacct gaaaatgaag ttgagatggt cattaaaatc ttctaaaaaa gaagcgttga
    44521 aggagaaata ttcctttcaa cgctcttttt tatatgctta gaaacataaa taaattgtat
    44581 actttctttt tagcccctat tacttttcac gaaaaatatc tttttttcga cgctagcgtt
    44641 tctctacttc taatactgta cacagttttt gtattattga aaaattactc attatttagc
    44701 attaatccac atcgttttat caatcgacct tcatactaaa ctactttaaa gtcatcttcc
    44761 ttctcgtttt tcattaaatc agatacataa gcccatgtac ccgttcgttg taaataaagt
    44821 tcttcttgta acaactccca ttttctctta gaaatcataa tgacgccatc gttcaatcca
    44881 tttttagaat ccctagtaat ttctatttca gaatttgaat gaactacttc ttgcaaca
//
LOCUS       DACDYM010000017        41531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDYM010000017 DACDYM010000000
VERSION     DACDYM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.017"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            214..498
                     /locus_tag="IWM59_000930"
     CDS             214..498
                     /locus_tag="IWM59_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9978978.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IWM59_000931"
     CDS             complement(779..1198)
                     /locus_tag="IWM59_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9978979.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="IWM59_000932"
     CDS             complement(1200..1784)
                     /locus_tag="IWM59_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9978980.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IWM59_000933"
     CDS             complement(1831..3306)
                     /locus_tag="IWM59_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9978981.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IWM59_000934"
     CDS             3487..3798
                     /locus_tag="IWM59_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9978982.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3914..6250)
                     /locus_tag="IWM59_000935"
     CDS             complement(3914..6250)
                     /locus_tag="IWM59_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9978983.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6392..7060
                     /locus_tag="IWM59_000936"
     CDS             6392..7060
                     /locus_tag="IWM59_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9978984.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7063..8106
                     /locus_tag="IWM59_000937"
     CDS             7063..8106
                     /locus_tag="IWM59_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9978985.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8343..9224)
                     /locus_tag="IWM59_000938"
     CDS             complement(8343..9224)
                     /locus_tag="IWM59_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9978986.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9383..10111
                     /locus_tag="IWM59_000939"
     CDS             9383..10111
                     /locus_tag="IWM59_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9978987.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10267..10584
                     /locus_tag="IWM59_000940"
     CDS             10267..10584
                     /locus_tag="IWM59_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9978988.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10577..11185
                     /locus_tag="IWM59_000941"
     CDS             10577..11185
                     /locus_tag="IWM59_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9978989.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11201..12031
                     /locus_tag="IWM59_000942"
     CDS             11201..12031
                     /locus_tag="IWM59_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9978990.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12169..12657
                     /locus_tag="IWM59_000943"
     CDS             12169..12657
                     /locus_tag="IWM59_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9978991.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12708..13580
                     /locus_tag="IWM59_000944"
     CDS             12708..13580
                     /locus_tag="IWM59_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9978992.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13756..14118
                     /locus_tag="IWM59_000945"
     CDS             13756..14118
                     /locus_tag="IWM59_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9978993.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14127..14879
                     /locus_tag="IWM59_000946"
     CDS             14127..14879
                     /locus_tag="IWM59_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9978994.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14893..16695
                     /locus_tag="IWM59_000947"
     CDS             14893..16695
                     /locus_tag="IWM59_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9978995.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16972..18012
                     /locus_tag="IWM59_000948"
     CDS             16972..18012
                     /locus_tag="IWM59_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9978996.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18064..20208)
                     /locus_tag="IWM59_000949"
     CDS             complement(18064..20208)
                     /locus_tag="IWM59_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9978997.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20565..21938)
                     /locus_tag="IWM59_000950"
     CDS             complement(20565..21938)
                     /locus_tag="IWM59_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9978998.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21938..22333)
                     /gene="rbsD"
                     /locus_tag="IWM59_000951"
     CDS             complement(21938..22333)
                     /gene="rbsD"
                     /locus_tag="IWM59_000951"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9978999.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22361..23278)
                     /gene="rbsK"
                     /locus_tag="IWM59_000952"
     CDS             complement(22361..23278)
                     /gene="rbsK"
                     /locus_tag="IWM59_000952"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9979000.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23386..24399)
                     /locus_tag="IWM59_000953"
     CDS             complement(23386..24399)
                     /locus_tag="IWM59_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9979001.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24480..25079)
                     /locus_tag="IWM59_000954"
     CDS             complement(24480..25079)
                     /locus_tag="IWM59_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9979002.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25265..25966
                     /locus_tag="IWM59_000955"
     CDS             25265..25966
                     /locus_tag="IWM59_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979003.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25976..28687
                     /locus_tag="IWM59_000956"
     CDS             25976..28687
                     /locus_tag="IWM59_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9979004.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29138..30766
                     /gene="treC"
                     /locus_tag="IWM59_000957"
     CDS             29138..30766
                     /gene="treC"
                     /locus_tag="IWM59_000957"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9979005.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30826..32007)
                     /locus_tag="IWM59_000958"
     CDS             complement(30826..32007)
                     /locus_tag="IWM59_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9979006.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32259..33782
                     /locus_tag="IWM59_000959"
     CDS             32259..33782
                     /locus_tag="IWM59_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9979007.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33851..34579)
                     /locus_tag="IWM59_000960"
     CDS             complement(33851..34579)
                     /locus_tag="IWM59_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9979008.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34592..35872)
                     /locus_tag="IWM59_000961"
     CDS             complement(34592..35872)
                     /locus_tag="IWM59_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9979009.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36013..36615)
                     /locus_tag="IWM59_000962"
     CDS             complement(36013..36615)
                     /locus_tag="IWM59_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9979010.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36860..37066
                     /locus_tag="IWM59_000963"
     CDS             36860..37066
                     /locus_tag="IWM59_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979011.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37378..38097
                     /locus_tag="IWM59_000964"
     CDS             37378..38097
                     /locus_tag="IWM59_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9979012.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38178..38861)
                     /locus_tag="IWM59_000965"
     CDS             complement(38178..38861)
                     /locus_tag="IWM59_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9979013.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39079..41265
                     /locus_tag="IWM59_000966"
     CDS             39079..41265
                     /locus_tag="IWM59_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9979014.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13482 a         7354 c         8324 g        12371 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaaacagc gcgaaacaag gaacatatta
     3841 cttcctcgtt tcttgctgtt ttctactatt tcagcgctct tatgactcaa atctaaatgt
     3901 ggttgtcaca accttattct gcacgcaaag cgatagcggc gtcttttttc gctgccattc
     3961 gtgcaggaag atgaccgcca agcatcgtca atatagtaga gacgatcact agaataatcg
     4021 catggacagg atttagttga gcaacatttt ttagatccgt catgttgtat aagatgctat
     4081 tgatagggaa cgtagctagc catgcaataa acacacctaa tatcccagaa gaaataccta
     4141 aaatacacgt ttctgcgtca aatacgcgag tgatgtcttt tttacgtgca cctaaagctt
     4201 ttagtacacc gatttctttt gtccgttcaa tcacagatgt ataagtaatg atactgatca
     4261 tgatcatact tgttactaaa gagatccctg caaaagcaat aaggacataa gtgatagcat
     4321 ccatcaatcc gccagtcagt tcagtcatcg ttccagctaa atcagtataa atgattttat
     4381 cttcatctga tttgccttta ttgtaatcat ctagataatc caagatcttt tctttgtctt
     4441 caaaattgtt cggataaatc atgatacttg aaggcaagct gtctccacct aaataactaa
     4501 gcaatgtttg tttggcattt tcgtcaactt tttcagtagt taagacattt acaccactgt
     4561 ctctttgagc tttgacgatt tctgattctt tgttttcgtt gacgatttga gtcgttaatt
     4621 gatcgctata agcgattcct ggagataaga gattcattgt agaagaagat ttcacgcgta
     4681 ggatccccga gatcttcaat tcatcagaag cattctgata catcgcatca taatctgtat
     4741 tagggataaa attcccagtt ggaagttttg tatagtaagt attgttgttt actaatttga
     4801 atgttgtacc aacgatatca tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa
     4861 gattcttcaa tgcgttgata ttagtattat tattgccgtc tacgatcagc acgacatctg
     4921 ttgctgaggc tggataactt ccggcaagta aagaataatt gtcttttaaa aagttttctt
     4981 tgctagtgtc aagctgtgta gggaaacttg acactcccac acctgtcatt gctgacatcg
     5041 tactagataa agaaagagat tccgtatcag gattttgatt cgagaagctg acaggctgta
     5101 ctttgccatt tacatcacgt aaaagattga ttcctgtagt acgggtaaat ccgatattat
     5161 tacttaagtt aggatcgatg tctgttacgt aatcgacgta tgtttgatca atcttattcg
     5221 tgtgctgtgc tttatcttct tcgctgactt ttgcagtaat cgttttgctg tctggataat
     5281 ccgctttgct tgcacccaat ccggcatcgt cacgagtctg gttagtcgtt accttagaaa
     5341 tagtaatcgg aaatttcgcc atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc
     5401 cagaagaaag ggacagaacg atcgcgattc cgatgatccc aatactggaa gcaaaggagg
     5461 tcaagaaagt acgtcctttt tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc
     5521 aaaaactcat ctttgttctg cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag
     5581 gatgggaatc tgttaagatc ttaccatcag aaaattcaat gattcgatct gcgtattgat
     5641 gggccagttc aggattatgt gtgaccatga tcactaattt ttctttcgat aactcttgga
     5701 tcaattccat gatctggata cttgtttctg tatctaacgc tccggttggt tcatcgcaca
     5761 aaaggatatc tgggtcattg gctagtgctc gagcgatggc cactcgctgc atttgtccac
     5821 cagataactg atttggcttt ttatgcatat gatttgtcaa gccgactcgg tgcaatgcat
     5881 cttctgcccg ttttcgtttt tcgtctttag aaacaccact taatgtcatc ccaagttcaa
     5941 cgttttcaat aatgcctaga tgaccaatca agttgtaact ttgaaaaatg aaaccgatag
     6001 agttgtttcg gtaggcatcc cagtcactgt ccttaaagtc tttagtagac tttccgttga
     6061 ttaccatatc accggaatca taattatcta gtccaccaat cacattcagc atagtcgttt
     6121 taccagaacc actaggacct aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca
     6181 caccatccaa tgctttggtg gtggtttcgc cgactttata atattttttt atatctttta
     6241 attgaagcat aatcaccctt ctcccgtatt gaaatattcg gtatactaat aagtatatca
     6301 gaacaatgat cgtagtatac gaaataaatg ttaactgaag atgaactaca agaaaaaatc
     6361 tagaaatttt tcaaaaaaca gagggagatc aatgaatcgt attttagtag tagaagatga
     6421 tccgcaatta cagttgcttt accgttctgt gttggaacga gcgggctttt cagtcatcac
     6481 cgcattgaat ggagaggatg cgctgaaaaa actagaagcg acacgtatag aattaatcat
     6541 tacagatatc atgatgccga aaatggatgg ctatgacttg ctagactcat tacgtagcac
     6601 cagatttgac acacctgttc taatgattac ggcaaaggcc gattttgagg ataagaaacg
     6661 cgggttcaaa ttaggagcag atgattatat gacaaaaccc atcgatgtca atgagatgat
     6721 cctacgtgtg gaagcattgc ttcgacgttc aaagatcaat tacagcagta gcttgataat
     6781 cggacaaaca aaattagagc tagatgctta tacagtgtta caagatcagc attcgttgat
     6841 tctgcctcaa aaagaatttc tgcttctgta taaattactg tcttatccca acaagatctt
     6901 cactcgccaa cagttgatgg atgatatttg gggattagat acgaatacag aagaacgaac
     6961 gatcgatgta catatcaaac gtttaaggac ccgttttgaa aagacggaag attttcaaat
     7021 catcacagta cggggactag gctataaggc ggttatttaa agatgaaaaa aaagctattt
     7081 tcacaattat ggatctattt tgctagtatt gtcttcattt cgatcttagt gacactgctg
     7141 tgttttctcg gttttatttt cttattgtct cgacattcta tctcgcctgc tgaatcggct
     7201 ggaaaaccaa cgttataccc gttattcgtc tttgcggggt tcagtatggt cgtgggtaca
     7261 ggaatatcga tttttgttgg gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat
     7321 atgagtctgg tagcaacggg agatttttcg attcgaatgg atgaacagca gaaagtagca
     7381 gaagtacagc aactgtataa agatttcaac gttatggtcc aagaactcaa tagtatcgag
     7441 acactgagga atgactttgt gtccagtgta tcccatgaat tcaaaacacc actagcaaca
     7501 atccaaggat atgttcaatt gttacaagcc ccaaatctct ctgacgaaga acgccaaata
     7561 tttttatatc ggattatcga aagtatcacg cagctttcac aattaacaga gaacatctta
     7621 aaattgaaca aactggaaaa ccagcgtatc caactggaaa agaaagagta tcgattagat
     7681 gaacaaatca gagaagtaat cgtttttttt cagcctaaat gggagaaaga acagctagaa
     7741 ttggatatcg aacttgctgc agtaaattac acaggcaatg aagagttttt gtaccaagtg
     7801 tggctgaata tcatggataa cgccataaag tacaatcaaa tcaatggaca gattcatatc
     7861 aaactatttg agacagccac agagattgta ctggaagtga ccgattcagg tgtaggaatg
     7921 aatgaagaga ccagagatcg aatgtttgaa aaattttatc aaggagatac aagtcgtcaa
     7981 atttctggga atgggctagg actttcactg gttaaaaaaa ttctagaact gcatgatgga
     8041 cgtattgatt atagcagtat cgaaggagta ggaacgacag caatgatacg gttaagtaaa
     8101 caataggaaa aagaataaaa cccctaaaaa ttctattgaa ggtttttagg ggtttttagt
     8161 cgtttttttg tgattaggga aatgttctta cggttctttt ggcataaaaa tcgctgcttt
     8221 cttattgaac tacgagaaat gttagaggtg tcattgaaac aaggacatga tgcaagaaag
     8281 agagcgaaag tcatgcgtcg ctctcttttt ttgggtggat agatggacag aaaatgtagg
     8341 gcttagtttt cttcttgatt taaatactcc caaaatgtat ctgaagccaa agaatgctca
     8401 ccgactttac gagtaaatgc aagataactc tctgttgttg gagaaatagg gagaatcaca
     8461 gtattttctt ttaatagatt cttgactgtt ttttccataa caatagcaat ccccatgtca
     8521 tttgcaatca tattgatgat caagtcgatt cttgctcctt tataagagat ttttggttca
     8581 aatccagctt cgcgacacag atctagtaca ggttgataca agttcgtagt ggggccaagg
     8641 agcaaaaagt tctccttttt cagttcgctt agatccaatt tctttttact agctaaagga
     8701 tgatttttag gtagaactgc tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa
     8761 cttggtgggg cctggaaact tctagcaaag tggatcgtgt cactatgttc attggcgtca
     8821 ttgtaagtta attcgacact ttccacttct ttcaactgga ctgtaaattc aggatggcgt
     8881 ttcaaaaact cggtgatttt ggaaaaacct ttataattga tcattgtcgg tatcgttaag
     8941 atatttaatg aaagattgtt gtctttcgta taggtttcaa gcacttcttg catagcatgg
     9001 tattgttcta cgacattttt tacatatggg agcaataagc ttccagcttc ggtcaattcg
     9061 atttttctat gggcgcgttc gaataatttg acgtctaatt ctgtttctaa cgaaagaatc
     9121 tgtttagaaa tatttccttg ggtggtgaat tgtcgatcag cagttttggt gaaattcatt
     9181 gtttcggcta aatcaagaaa aacttcgaat ttatggatat ccatcatatt cctcctactt
     9241 attccaataa agaatacttt aacacaaaaa atgaattggt gtaatcttct ctgtctgtta
     9301 caatctttct tgcagcgaaa gcaataatta cagaaatttt tctgattttt ttcgcttcgt
     9361 agaaaaagag aggagttttt tgatgccaac tggagttttg atcaatgtag gttccgtttt
     9421 gtttggcgga ctaattggcg gattagtagg aaataaatta agtgaacatt ttaaagcaca
     9481 gctgacgatg gtttttggtg tgtgttcgat ggggatggga atctattcca ttgcgccgat
     9541 gaaaaatatg ccggctgtta tttttgcttt agtgatagga acagcgattg gtttgattgt
     9601 tcatttaggt aatgggataa ataagggggc tgcgctgatg caagtaccta tttctaaaat
     9661 cttccctagt gaaaagcttg gtatgaccca cgatgaattt atcagcacat tagtgacggt
     9721 cattgttcta ttttgtgcaa gtggtacggg gatctatgga agtttagatt ctgggatgac
     9781 aggagacagt acgattttga tttctaaatc tgtccttgat tttttcacag cagctatttt
     9841 tgcgtgtaat ttagggtatg tcgtttctgt gatagcaatt ccccaattca ttatttttta
     9901 tattttgttc ttattggcaa aatttattta tccattgact acaccagaca tgatcttaga
     9961 ttttaaagct tgcggcggat tcttgatggt cgctaccggt tttagaatga tcaacgtgaa
    10021 aatgttccca gtagctgaca tgatacctgc aatgatcgtt atcatgccac tgagctggat
    10081 gtggacaaat tggattatgc cactgttata ataaaaatca aagagaagcc attttttgct
    10141 gactaaaaag gatcaaatct tttagtcagt ttttttgtat ttgacaaact gattttgaaa
    10201 ctatattata tgttgtataa tatagattga aataaaatat tacacttgag aatagaaggt
    10261 gacgtaatgc acatccagat tccaactgtt cttcttgatg gtactgtgct agcagcatta
    10321 agcaaggaag atatgtatgg gtatgcctta acaaaaatcg tacaagaaag tctctctgtc
    10381 agtgaatcta cactatatcc ggttttgaga agattgaaaa aagaaggata tcttgagacc
    10441 tatgatgaac cttttgaagg cagaaaccgt cgatattact cgttgacacc aatgggaagt
    10501 cgtcatttag aagaaattat tgatgaatgg attgtttttc gagattcttt agaccggtta
    10561 ttggaggaga gcacaaatga atgaatattt gaatgaagtt gtggatcatc ttcaagaaat
    10621 tcctgaagac gatcgttttg atttgatcca gtattatgag gaatattttt tagattctgg
    10681 aaaaacatta gatcagatca ttgatgaata tggtacacca aagcagtttg ccttaaaatt
    10741 aaaaatcagt tacttttcag aagaagaaca agcagaaaac gagttagtga aaaatccaaa
    10801 aagacaaatt cgcctgattt ggttaatcat tatcggttta tttgcttctc ctgtcctcat
    10861 tccactagca ctggggttta tattggttat tgggggacta ttggttgctt tactagcgct
    10921 catttttagt atctatgttt tatgtatcag tatcttaggt gttggcctat tcatgatcat
    10981 ctcaggaatc agtgtgtttg gacaatcgat tctgagtgga ttattcttta tcggtgtcgg
    11041 tctgttcgcc acaggaagcg cagtatttat ttcgccactg ttgctgaaag gaacaaaagg
    11101 gcttttttat gtcatgatga actttgttaa atggatggga cgacgtttta ttacaaaaag
    11161 agatctgcat tcagcgggcg tttgaaggag gacacataaa ttgagattga aacatttttt
    11221 gattaccggt attggtctga tgcttactgg tggcctccta gcaggtgcaa cttatacatt
    11281 aggcgctcaa aagagtctga cttgggataa tggtcctcgg ttgatcgata tggttactga
    11341 gacaaaaaaa atcaaggatg caaacaaaat cgtcatccat gcagaaaatc aaacagtttc
    11401 ggtcatacgc ggattggatt ttgagataaa aacgacttat gataagacag aaaagccaga
    11461 aataaaggag aaaaatggcg tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat
    11521 cattagttta gaagacacaa gtgcacaaat cactattgtt gtccctgcag atcatgaatt
    11581 atctgaattg acaatcgaag ggaataatag tgcaattgat atcaacgaag tatcaatcaa
    11641 aaaagtgaat actaagacac atgatggctg ggtatcgttc aatgaattta caggaggaac
    11701 aataactgcg aaagatgaat taggctcact tcgattagat gaggccaatg tggataactt
    11761 gatcgtcaca ggtgaaaaca gcaacttcca tctagaagac agtttgatca gcaaagaagg
    11821 aaaagtcatc ctaaacggtg gcaatttatc tttgcatgag atggaaaaca gtggctatac
    11881 tatccaaacg tctggtgaat cttatattga aaaagactac cagccaataa caaatacttt
    11941 gttagaacaa ggacagaaaa agctaaagat cactgctaaa gacacatcga tcaatattac
    12001 gacggatcaa gaggacaatg aaacagattg atcctgtact caactggata ttttaagaaa
    12061 aaacagcaaa gatccttcca atctttgctg tttttcatcg cttaacgcac ataactatag
    12121 taaaataaga aagaaaatat ctaaaaagaa aacagggtga aagagtatat gaaggtagaa
    12181 gaaggtcaaa gagaaaagtt aaaaacagaa atggaaagat tacatactta tatcactcaa
    12241 ctaagccaaa ccttctatga tccagacaaa gaaaaagtga tggttaatta cccgaacaat
    12301 tcagaaggtc gccaattaga gcaagtgtac catgaagttt tcaaacattt attgacggtg
    12361 aagaaagagc tggattatta ttctttaccg attatcgata caggaatatt gaagtatgat
    12421 gggaagaagg aacgatttat ctttaaatct gttcgggaag atttgcctct ttcagcaggg
    12481 atggatctag aggtcttggt agaagactat tttacagaag aaaagcactg ggttcgaaca
    12541 aaattggatt atttaccgca ggcagccgga gggacacagg caagcggttg gtacatcact
    12601 gaagacaaag agttagaact agaaggtgta atggcgcgta ttcgaaaaaa aaattaaaaa
    12661 agtaacatta gatatttatt gaaggataaa ggagcgcagg aagtagcatg agagaaaaaa
    12721 gagcatttta tatcaatggt tatgcaggga ttgtcggatt ggtcgtatta gctttagttg
    12781 gattgttttt cttctattta ggtatgtggc aagcgaaagt ttgggcactt tttttaagta
    12841 tttttctttg gttgatcacg ttattgttgt taagttcggc aactgtagtc agtcctaatc
    12901 aagcaaaagt aattttgttt tttggacaat atttaggtac gattcgagaa aatggctttt
    12961 tccttacgat acctttggca caaaaaatga ctgtttcgtt gaaagttcgg aatttcaata
    13021 gttctgtttt aaaagtcaat gatttggatg gaaaccccat cgaaatctca gcagtagtag
    13081 tctttaaagt cgttgataca gcaaaggcat tattcgatgt agcgtattat caagattttg
    13141 tagaaatcca aagtgagaca gccattcggc atatcgctag ccaatacccg tatgatacat
    13201 tcaatgagaa tgatttgacg cttcgaggaa acacgacggc agtgtctgac gagttgcaaa
    13261 aagagctgca agagcgttta gcagtagcag gtgtcgaagt aatcgaaaca cgcttgaacc
    13321 atttagccta tgcaacagaa atcgctagtg ccatgctcca acgtcaacaa gcaaaagcca
    13381 ttttgtctgc tcgacaaacc attgttgaag gagctgtcac aatcactcaa atggccttag
    13441 agcaaatcga agatggacaa gaaatcaatt tcaccgatga tcggaaagtc caattgatca
    13501 ataatttact tgtctcaatc attacagaca aaggaacgca acctgttatc aatacaggcg
    13561 acatgaaaga ccgtaagtga catacttttt gtaagatagg aagaaggagt ttaggacgga
    13621 ttattgtctt gaactctttt tttagttgtt ttaaagaaaa tgacaaaatt gaaagaagaa
    13681 tgatagttga ttcgaaggca gaattgttcg cttttttcta taatcagtat ataaagtaag
    13741 agagaagggg aaaaagtgaa aaaacttctg atcggtctac ttgttttcat cgttgtggta
    13801 cttgcagggg tgaaaatcgc agattacgta gttatgggtg gagaagaata ctatgtacaa
    13861 attacgacag atggtacaaa aacagtttca aaatcagact ctggacaaga aacagtcaac
    13921 tatagatata tcctgcctgg ctatacgaaa gagggtaagg aaaaagaact ggacttcaag
    13981 ggacaattga ctcgaccgct tcgtaaagga gcatatttaa aaatcacctg gaacaaaaat
    14041 aaaggtgtca cttcgtatga agaagtcgaa aaaaaagacg tgccgaaaac agcactggaa
    14101 aaactaatta aagggtaggg aaagaaatga acaaaatagt cgaagtacag aatgtaacaa
    14161 aagtatacgg aaaaaacaat gagaaaaaga cgcaagcatt aagcggcatc agcttcgatg
    14221 tagaaaaagg cgaatttatt gggatcatgg gcgcttctgg ttctggtaag tcgacacttt
    14281 tgaatatttt atccacatta gataaaccaa cagatgggca tatccgaatc aatcaaaaag
    14341 atgtgaccac attaaaagga aatcaactag cagattttcg tgcgaatgag attggcttta
    14401 ttttccaaga tttcaattta ttagaaagct tgacagcaca agaaaatatc gctgttcctc
    14461 tttctttaca aggcgtcaga ccaaaagaaa tcaagcaacg tgtccaaaaa attgcagaac
    14521 gtttatccat ctctcatatt ttggaaagct atccttctga gatttccggt ggacaaaaac
    14581 aacgggtcgc cgccgcacgt gcattgatca cacaaccaac gattctatta ggggatgagc
    14641 ctacaggtgc gctggattcc aaaagtgccc gggatctttt agatacgatg gacgaattga
    14701 atacaaaaga tcatgtatcg attcttcttg tgacgcatga tccgttttct gcaagttatt
    14761 gccaacgtat tttattcatc aaggatggcg gtatccatca agaagtgaaa cgtggagatc
    14821 aaagccgtga ttccttctat cgagaaatac tatctattct tggaaacttg gaacagtagg
    14881 gggagattga ctatgctttg gaaattatca cttactggga tcaagagccg tttaagagac
    14941 tacttggtgt tattctcagg attagttatg gcatctgcta ttttttacat gtttgaatca
    15001 atggcaagca acgaagcttt tttgaaaagt aatacgatcg ttgcggctac agttatcatt
    15061 ttccgattcg gttctgtttt actagggatc atcacattcg tttatatttt atatgcaaat
    15121 tcctttttga tgacgatgcg gcaaaaagac tatgcaatgt ttatgatgct gggagcaaaa
    15181 ggcagaaaaa tcgcacagat gattttttct gaaacattct tagtagggat cgcagctact
    15241 ttagtgggtt cagctgtcgg tgtaggattg acaagtatcg tccaccgatt attagtggat
    15301 cagttagatc tcaatatttc gcattttaca ccttttagta ttcacgggtt gttgatcact
    15361 gttttgtttt ttgctgtttt gtttttatta gctgctgttg tcaatgcttt ttcgattgtc
    15421 aaaaaaccga ttttagcatt gattcgtgcg gatcagacac caacaagaat caagcagcat
    15481 aaattcttgt ttttccttga agtagtactg ggaattgctt ttctaggctg cggttactat
    15541 atgatgaaac acgtggggac ctatcaagtc tttggtatag gggtagcctt agttacgatc
    15601 gtattaggaa cttatttcat tttccattct gtgatcattt tctttttatc attactaaag
    15661 aaatcagaac agatttcatt gaaaaaattg aacaatttca cattgtcaca gttaaacttt
    15721 cgtattcgtg aatatacaca aatgctctca atggtcgcta tgctgtttgc cttggcactg
    15781 ggcgctttga cggtaggatt aggttttaaa aatgaaattg cagacttgac agaaaacaat
    15841 tctggatatg atctagtgat gaataatcct cgggcagaag atcagcaaaa aatcaaagaa
    15901 ctttcaccaa cactttctgc ttcttatact caaaaagaag atgcgggaac gatttactat
    15961 aatgcaagtg aatttgcggc tcaaccattg atcatgataa aacatgaaga tggaacaccg
    16021 ccgaagatca cgaaagaaaa agttcctgct aaaaaaatga atgaactgac tgtacaagag
    16081 gaacttcgaa gctatgaatt gccggaacag caagaaaaag aaatcaaggt agtcagtcaa
    16141 tcagaatttg atcaactgaa cttgccacaa tccactttac agctagttca ggtaaaagac
    16201 ttccaagcaa acctgaaacc aatcgaaaca cttgttgctc aaaataaaat gaacaatcca
    16261 tctttgcaaa aagtagagta cagcaaccag aaatatgaaa tgtatgaagt gtataatggt
    16321 ctattctctg gttttgaatt tatgggattc ttcctaggtt tagctttctt gaccatgctt
    16381 gctagctgtt tgatgttcaa gatcttgtca ggaagcaaga gtgacattgc tcgttatgaa
    16441 atgcttgaaa agataggaac acgacgcggg ttgcttaaac aatcgatccg tagagaagta
    16501 ggcgtgttat tcttagcacc aggaatctta ggtgtagtcc atgtcttatt cggattacaa
    16561 atgtttggtc tattcatgca aaatccatac aaagatattt gggttccgtt tacgatattc
    16621 ttcgtattgt atttcatcta ctatgtactg acgacatggt tatatactgg aatcgtattg
    16681 aaaaagagat tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca agcaaaaata
    16741 gctcctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    16801 tttgttcgga aaaagaggtt gtgacaatat ttttgtcaca accttttttt cacggtcttt
    16861 tattagaata tagtaaattt tttctcgtta ctcacggaat aagctgttaa gaaaacggtt
    16921 tccatgtata ctggaatcag tgcaaatgaa agaaaagagg gtgcaaggaa catggaaatc
    16981 aatgaacaaa attttggcga aaactatcac ttgattacga tcacgaatca aaatgggaca
    17041 tctttgtctg tcagtgatct tggggcacgg gttgttcatt ttcaaacaat aatcgataat
    17101 gaaaaacgag agttgatctt aggttttgat gaagcaaaag aatatctaga aaaagaccct
    17161 tatatcggtg cttcaatcgg acgtatggct ggacggatta cgaatggcac attcaaatta
    17221 gatgatgaaa cgttcgaagt aaaaacagac ccggacactg gtcattgttt acatggtggt
    17281 gcacctagct ttgaagccaa gcagtggcat tatacagtca tcaatggtga aaacgaagca
    17341 tcggtcattt tctatcagac tagcccagat atggaaaacg gatttcctgg aactttagat
    17401 gtagaagtac ggtatacgct gacgaacgat gatacatggc gagtgacggt ccaagggatc
    17461 agtgaccgca aaacagtatt caatcctaca aatcatgtct atttcaattt aaccggtgat
    17521 ccagcacagt caatcgacga acatatactt tggttgaata gccgattatt tgctacgctc
    17581 aataaagata caacgcctac aggagaagta gtttcagtaa gtggcacagc atttgacttc
    17641 caacgtccta aaaaattagg tgaggtcttt gccagtgatt ttcgccaatc aatgatagtg
    17701 ggcggcatcg atcatccatt tttgttgaaa aaacaatctg gtttactaaa tgctgcggaa
    17761 ttgacaagcc cagatgaaaa agtatcgatc caagtaaaaa cagatgcttc aggaatcgtg
    17821 atttttacag caaattttgg agaagatgga ccggaaatgc gcgggaataa attagtaaat
    17881 catggcggta tcacatttga gactcaagaa ctgccaggag cggagcgact tgaaagtttt
    17941 ggagatgtca ctctggaacc taaccaagta cgcgagtttg taacggaata caaaataaat
    18001 ataaagaaat agaataaatt aaaaagggag tgtgacagtg tcacatcccc tttttaattt
    18061 attctatttc tttaaaggca gatcaagcca gaatgatttg tctaaactta acaaggttag
    18121 tacaggaaca gttgcagctt taactgtacg agtagttgtt tctggtatct gtcttgccgt
    18181 aaaattgttc cacaaatccc agattgaagc gacacggaag atggaagaag cgttgttgac
    18241 gcgttttatc gttgaaaatt ctaatcctgt gattggcacg ccatctgttg tccatttgat
    18301 tggcacatcg aatcgcttag acatattgag gatttcagcg tttgcttctt cagttgttgc
    18361 tgcttctttt ttcggttcat catattttgt caggtcgtac gcttcgatga tcgcgttcaa
    18421 tttttgtgaa tattggcgat ctgtagcata acgtccttcc aaatatttgg ttgcttcttt
    18481 gtagtctttt gtttccgact tccaagttgg tttgtagaaa tcaggattgc cagtcagtcc
    18541 atttttgatc aagtcggcat agtcttccaa actttccttg taagaaggat atttacggaa
    18601 ttgtgcgcgg atttggtagc tttgtccgga actatcttgt tcaagtgtat tgaaggtcac
    18661 gctgcttcct ttatatgatc ctttgatacc aaaaaggtta tgatttggtt cgcttgataa
    18721 taagctgttt ccagatccac tttcaaggat agcttgagcc atcatgactg atgcgtagat
    18781 tccttctttt tgcccgattt cctgtgcatc atctgcaatc tttttcaaga actgatcagt
    18841 tgtttggttt tgactgaaat ggaacgagcc atctaatcca tctaaaatag atgagccgcc
    18901 tgtcagcgaa cctgaagcag caggtaaacc tgctaagttt actcggacag caaaatcagg
    18961 cgtgaaatct gaaatagaag ttactcgaac tttatctcct ggttgaggcg catggataaa
    19021 ttgtccgttc ccaatataga tacctacatg gtgagtaaca ccggaagaag gtccccagaa
    19081 gacgaggtcg ccaggttggg cctgacttac tggaatctgc gtacctgcat attgctgttc
    19141 gcctgtccag ccaccgatat aatgaccggt tgctttcata tatacgtaat aagtcaaacc
    19201 agaacagtca aaagtatttg gaccttttgc tccccataca tatggttttc caagatgctt
    19261 gaatgcttct cgaacgattg cttgttggac attcgttgta tttgtaggca atacagcatc
    19321 aattggtgat tttacaacaa aatcagaaga agcagaagat ggattctcta taccgctttg
    19381 atttgctgta tttggacgag atgaagcatt gttcgttccg ccattcgatg aaccattgtt
    19441 tggatggttt ggttctctct gagtatttcc attgttattc gatccagatg ggtttgtttg
    19501 attcgaagaa ttcccgctgt tcgaattatt gttattattg tttgaattat ttgaaccatt
    19561 attattgttg ttattaccca catctggttt gtttggcgtt tccggatctg gtgtactagg
    19621 gacttcttca ctaggatcag ttggtttctc tgttgaagta tccggtccag ctttaaattc
    19681 tgcagtaata gataaattgt tcgtacctac tgtaaaggtc tttgcatcat aatttacttc
    19741 tttggcacca ttaacagcga ctgaagcaat ttgttcacct tcagctggcg taatagtgta
    19801 agttaccttc gtttccgcta ataaaccttc taccgttcca ctttccgtaa tatccaaagg
    19861 ttgctgttca ggggaatccg ctgtcgaagc agtcagttgg atcttaccaa tcaaagaagg
    19921 agcaattgta agggaatatg tctcggctgg cttttcattt tctgatggat cagccggatc
    19981 aatttcagtt ggctcggtcg atcctgttgt tgagttagtt gttccagttg ggtcttctgt
    20041 aaaagaatcc gttgtttcac tagtagctga gctggatgta gtcgtatctg ttgtgactgt
    20101 ttgatcagct atttgattat ctacggcaaa tacggtattg gtcgacaaaa cagacggagc
    20161 aatcagccaa gtagcagcta gcaaacgtat cgttttttta tttttcataa taaactagtc
    20221 cttttccttt cattgatttt ctgtcttatt cagcattcat tagacatttt ttggaacaga
    20281 taatcgtgtc gaaaagcttt ttcttttcat aaatctgtct gtgtattcta aataaaacaa
    20341 tttgtaaata agcaacgagc attcattcgt ttttctttga cgttcatttt attgcataga
    20401 ccacctttgt ttggggataa acaagtgggg ataaaaatgg ttatgcatat gtaaattata
    20461 ccaaagtttg gaaagtatca caaaaattta taaaaataat gatcctaaaa agaaaaagac
    20521 tgaaatatgt gataaacatc tcatacttca gtctttttca ttaactattc ataagacatt
    20581 aggtcttcat caaacccgct aacgagagta gtgatcattg ctacgactag actaacagcc
    20641 agtcctgcta agtaccagaa aaaagtaccg gaaatgaatg caggcaatgt ggtcagacta
    20701 ccaaatacaa aagcgatcgc tttcacttgg gcaaatccca ttacagctcc accagcagct
    20761 gcaccaatgc aagcagcaac aaatggtttt ttcaatggga tcgcaattcc gtaaacagtt
    20821 ggttcggtca ctcccgcaag taaaatcgaa gcaaaagcag aaccggagat cgaacgggtt
    20881 tttgaacgtt tacttcgcag aaatacacct aaagctgcac cagccattcc aaaattggct
    20941 gctcctgtca acggaacgat tcggtcgaac ccataagcag agatattact gatcatgaca
    21001 gggtttacag cccattggat accaaatatt accaaaatat tccaaccacc agcgatgatt
    21061 gcgccactga tcggtccgtt aatatccatc atccaagtaa ttgcggcagc taaaccttcg
    21121 ccaaggtaaa cacccgcagg cccgaatacc attaacgtca aaggaaccgt gataactaat
    21181 gaaagtaaag gtacaaatac taattgcata ttttcgcgaa tatatttttt gaggaatttt
    21241 tcaacaaaag aataaaagaa aatacctaaa atcgccggca tgacagtaga cgtatactgc
    21301 atcagtacta caggaatccc cagaaaatca gtagctgctt tgccactgtc tgaaagtaaa
    21361 gaggtcagat tcggttcaat cagtgctgca gcaatgacag ccgcaatata tccattcact
    21421 ttaaacgttt tggcagcaga aaaagcaaga aagattggca gaaaataaaa gacggcattc
    21481 gacgcagcag acaaaatatg ataagttccg cttgtttcac taagccagcc aatcgtagtc
    21541 gctaaaacaa ccagaccttt taatatacca gaaccggcaa acagcggaat gatcggcgta
    21601 aagatttccg atatcaaacc aaatagtttt tcgatcggat ttagcttttc tttcggagct
    21661 gaatgatcga tttgacgcgt caattccttg taagcaggag ccaatccctg atccatgatt
    21721 atttctgcat atctatcgag taaaatgaaa tacttaatgg aagggatttg ctgttttaat
    21781 ttttctgcat tggctactgg ctgttttttc aatttgatac gaagccgagt cgcacatgga
    21841 aggacagaat caatattgtc ttttcctccc aactgttcaa tcagcatatg aatctgctcg
    21901 gacagtacca tctgttcagg attcattgtc gtttccatta gaagaccact ccactcacta
    21961 atataatatt ggcgtaaggt gtcatttcgc ctgtacgaat aaatgcatga cagttattca
    22021 actctttttt catttcttca tgtgggataa agctaatcgg tgtatctggt aacaataatt
    22081 tgattttttc caatatttcc gggttcattg tctttatttc ttccgccaat tcaatcgatt
    22141 gtacctcaag ttcttctaaa acgttttcta aaacagataa aaaacttgga agaccgtttg
    22201 agacagccaa atctattttt tcagtaccaa aggggacagg catgcctgca tccccgatac
    22261 ttaatttatc aaagtgcccc atttgtgcga ttactcgtga aatatctgaa ttgattactt
    22321 ttcctttttt catgtttgtt tcttcctctc attgttaatt ttagactgct gattgttcta
    22381 aagcttcgcg gtaagggata gaaggttgcg caccataaga ctgcacagca acagaagagg
    22441 ctagatttcc ataaaggatc gcttctttca agttactaag gtctggctga agtgtgctgc
    22501 ttaatgcgcc aataaatgta tcacctgcgg ctgttgtgtc aacggcatcc actttaaaag
    22561 cagggacgat accgtgttct ttttctgtat gatagaaagc gccggcactg cctagtgtaa
    22621 tgattactgt tccaatacct aattcatgga gtttttcggc tgctgcgacc aaactgttat
    22681 gatctgtcac tctgattcct gtaatgattt ctgtttctgt ttcatttggt ataatgatat
    22741 ctgtcttttc aagtagttca gtaggaatcg tttcacgagc tggtgcagga ttcaggatcg
    22801 ttgtttttcc tgcgtctttt gcaatagaaa aagctaggat cgtcgcatct aagctacttt
    22861 caaactgagc aataataaag tcactgtttg taatcagttg cttgtttttt aggacttctt
    22921 gttcatggaa agcattattt gcccctgcat ggatcaagat actattttct cctgctgcgt
    22981 ctaccataat cattgcttgt ccggttgtgg cgccttggat ttcagctatc ccagatgtat
    23041 cgatgttttc ttttgtcagt agatctaata actgttgtcc ttcgctgtca gccccaacgc
    23101 ctccaataaa agatgtccgt gcgcctgaac gtttggctgc cactgcttgg ttggcacctt
    23161 tacctccgcc agcgtgaaaa atctcatgcg catgcatcgt ttcgcctggc ttgggcatgt
    23221 tggttaaacg aagggtagtg tccatattaa tacttccaat tactgtaacc gtgttcattt
    23281 tacaaacctc caactatctc atttattttg atgggaaaat tattttttaa aacgttttag
    23341 taaaacgttt taatagttcg ataaaaatat agcacacctt gaaagctatt tcaagggtgc
    23401 agttgaaaat cttttttcta atcgtacagg taatcttttt tcttcccatt ctttttctgg
    23461 gaattggatc cgatctaaca ataacttggc agatgtttgt cccaattcga agattggttg
    23521 tgcgatcgtt gtcagtttcg gcttgatata ctcgcacata tctatattat catagccaat
    23581 aatgctgtag tcttcaggga tgctttttcc agcttcctct aatccgcgat ataatccaaa
    23641 agctaattca tcatttaaag caaacacacc agttgcagca gaatcaagaa gttctgctgt
    23701 gatctggtag ccaccttgtt ttgagaactg tgtaggtaag aggatcagtt ggtcgtgagg
    23761 gatttggtaa acatcaagag cagatttaaa cccctcaatc cgagcgtgga cattttccgg
    23821 tggattttca ggatagacaa gggcgatcgt ttgatgtcct agactaagca agtgcatgcc
    23881 cgctaggtaa ccaccgcgaa aatcgtctgt tcgtacggca tcactaaatc cttcagattt
    23941 tttctgatcc aaaacaatga agggccggcc ttgttttttc aaattttcgt tgattgcatc
    24001 tgtagagaca gcagaagtcg cgataatgaa tccatccaca cctcgacgtg tgagctcggc
    24061 tagatattcg atttcttttt gatgatcaga atcagcatta cataatatcg tgacgaaatt
    24121 ctgcttatac aagatatcct caattcctcg catcagtgta ctgaagaatg gattcgtgat
    24181 atcaggaacg agtacaccaa ttgttttcgt ttccttggta atcatttgtc tagcaaaata
    24241 gtcaggctga tagcctaatt cttctttggc agctaaaact ttctgaacag ttttcggact
    24301 gaatttctcc gtatttccat ttaatatcaa ggagacagtt gtgattgaaa cgccggaaag
    24361 tttagcgact tctttgattg tgatcttttt tttcaccatg ttcatactcc tatctaaatc
    24421 ctgtggttat tttcccatga aatcaaaaag aagtaaatga aaatcacgtg tgccagtact
    24481 tatttgcttt gttggatagc ttcgattatt tgtttcgttg agcgtactcg tgcagttttc
    24541 ggaaatatct tttttatgaa caattgatgg gattctttgt cacgatcact gcaagcatct
    24601 tcagcaagca cgatatggta cccgtgttga aaggcatcta atgcagtagc atatacaccg
    24661 tttgttgtcg ctacaccgct taaaatgata gtttcgattc cacgccggcg aagttgaaga
    24721 tcaagagaag taccaaaaaa agcacttgga ttgtattttg tgatagtcaa catattatct
    24781 gtatctttca aagaatcctt caaaagtaaa tcagtgaatt ctggaggtac agaagcagtc
    24841 attccaccca tatcagtttg ttgactcaaa taagggaaag tggagatgtc tacgttcact
    24901 aaaactttca aggcagcggt acgtttcaat gttcttacta acaaatcatt gtttgtcaga
    24961 atagatgctg cagtgtgtgg agagagtgta ccgttattta cgatctcttt ttgcatgtcg
    25021 atcgcgacaa aagcagtttt tgataaatca aaaatcatat tttcctttaa atcattcatg
    25081 atgttccttc tttcaaataa caatgttttt tatgtaagta aatcataaat cgtttatttg
    25141 tcaactaaat taaaggattc ctttctttag tctagagaaa ttcagtggta aaataaataa
    25201 gtgtgagaaa acgcattcga tgcatagata tagcgagaaa tatcgtgaaa atggaggaat
    25261 cagagtgaag tatgtcgaag taaaaaatga atacaaacga tacaaaatgg gagaaacgac
    25321 cattacagca aatgacgata tttctttttc aattgaaaaa ggcgaattag tgattatact
    25381 tggtccaagt ggagcaggaa agtcaacgat tcttaatatt ttaggtggga tggatacacc
    25441 tgatgaaggt cagatcatta ttgatgacac agatattgcg caattttcag ataaacagct
    25501 tactgcttac cgacgaacag atgtagggtt cgttttccaa ttctacaatc tcgttcctaa
    25561 tctaacagca aaagaaaatg tggaattggc aacggaagtt tctcctgatg cgcttgatcc
    25621 tgtagaggta ttgaggcaag tgggcctagc tcatcgattg aataactttc cgtcccaatt
    25681 atcaggaggc gaacagcaga gggtttcaat tgctcgtgca ctagctaaaa atcccaaact
    25741 tcttttatgt gatgaaccga caggcgcgct ggattttgaa acgggcaagc aagtattgaa
    25801 attattgcaa aacgctagca gacaacacgg caataccgtt ttgatcatta cacataattc
    25861 tgctttagcg cccatcgctg atagagtcat tcacattaat gatgctaaag taaggtctgt
    25921 tgaattaaat gatcatcctt catccattga tgagattgta tggtaggggg attttatgaa
    25981 aacaagaaca tatcttaaag caggttttcg cgaaattcgg caatctaaag gacgattcat
    26041 tgcaatcatc cttattgtca tgctaggaac acttttattt gtaggtgtca aaactgctgg
    26101 acctgtcatg caaaaaacga tggatcacta tgttggtaca gcaggacttt ctgatttgca
    26161 aatcgtttct actggtggct tgacacaaaa agatattgct caagcagaaa agatcccaga
    26221 tgcaaaagta gaaacaggaa aacagatcta ttattccaat actggcaaaa acgaagtgat
    26281 tcaaatattt tcttataaca aaaataacaa gcagaacaag ctacaattaa ctgatggaaa
    26341 actcccggaa aaaccaaacc agcttgtatt ggatgaaaaa gcggaagatg aagggtataa
    26401 aatcggtgat atttatcaga ttgattcaga tgaattgaaa gaaaaagagt ataccatcac
    26461 gggctttgtc cgttctccat tgttcatcaa caatttggag agaggatacg cgaacgtcgg
    26521 aaatggtagc gtcgattatt ttgtctattt acctgaatcc gtttttaaaa cagatattta
    26581 ttctgtctta tatcttgatt ttacaaacgt aaagaatttg aatacgtaca gcaaagctta
    26641 caaagataaa atggagcaaa atcaagaaaa ggcagaaaag tatttacaag atcgacccga
    26701 agaacgctta gaagagctga aaaaatcggc caacgaagaa ttagaaccgg cacagcaaaa
    26761 aatagcggat ggcaaagcac aaacaacgca agcaagaaca cggttagaag atgctaaaaa
    26821 acagttagcg caacagtcag caactatcca gcagcttcct gaagcgcaac gaacaatcgc
    26881 tgttcaaaca ttatctcaac aagaagctca acttgctgaa caagaaaggc aattagttga
    26941 gaaagaacgt gaactggcct ctgcagagga agaattgaat cgaaatgcag aaaaaattga
    27001 aaatttaact aaaccatcat acctatacaa tgaacgttca gaaaatgtcg gttttcaaga
    27061 atttggtgat ctgtctgatc gtatcgctgc gattgccaat gtttttcctg ttttcttttt
    27121 ctttattgcc gcattgatca cctttacgac aatgacacgt atggtagaag aaaaccgcag
    27181 ggaaatcggg acattaaagg cgttaggtta tacgaaagtt gaaatcgcaa aaaaatatgc
    27241 aatctatgca agtctagcct cagggatagg tattgtttta ggaacgatat taggaacgaa
    27301 tcttttgcca aggatcattt ttgagctgtc taacgagcgg tatgatattg ggagtgcgct
    27361 tattttttat gactggccgc cgattttaca agcagctgcc gcattcttca ttgccgcatt
    27421 tggcgctgca atgatcgttt tattcaaaga tttaagagaa cggcctgctg cattgcttca
    27481 accgaaagca ccgaagccag ggaaacgcat ttttcttgaa tacatcactc cgctgtggtc
    27541 acgccttagc tttaatcaaa aaatcagtta ccgaaatctc ttccgatata agtcaagaat
    27601 gcttatggcg attatcggaa ttgctggatg tgccgggctg atggttgcag gcgtgggatt
    27661 gaaagattct ctaagttctg tttcagccaa acaatttgga ccgatcattg actatcaagc
    27721 aatcgttaca ttggattcat ccacggaaga tagtcgtcaa aaagcagcgg atatgctgca
    27781 gaagcaagaa aaagtaaatg agatcctagc tattcatact caaaccgtag aattgagaaa
    27841 aaaaggacat gcttcgcaaa gtctgacagt gatcgtacca agtaaaacag ctgatttgga
    27901 tcgttttatc catttaactg atcttgaaga gaacccacgc tccttgccta aaacaggaat
    27961 cgctattacg caaaaagtag cagaactatt tgacttatct acaggagatc atatttcgct
    28021 ttatgatgaa gatcaaaaag aatggaaagt cgaagtaaaa gaaatcgtga aaaactatct
    28081 aggtagcttt atttatatgt cccctgaata ttatgcttca gttactgatc aatcgatgat
    28141 ggataatgca tttttgctgc gtacagataa gatgacaaca aaagaagaag aaatgctttc
    28201 cgagaaactg cttgccacag acgctgtgac aaatacgtct ttcatgtcca aacaaatcga
    28261 aacgcaagaa gaatcaatgg cgaacttaga ctcggttgtc ttaatctttg tcgtattatc
    28321 aggactgctg gcttttatcg tcttgtataa tctaactaat atcaatattt ccgagcgtgt
    28381 acgcgaatta tctacaataa aagtccttgg attttttgat aaagaagtca cgatgtacat
    28441 tgttcgagaa aatatcatct ttactctttt aggaatcatc ggaggatttg gtgtaggata
    28501 tatattgact gacttcatct tgcaacaagc gtctatggag aatgtcatct ttccattagt
    28561 gatcacatgg tgggcttatg tcttatcagc aggattaact atcgtattta cggttattgt
    28621 gatgattgtt acccatttca aattaaagca tatcgatatg attgatgcgt tgaaatcgaa
    28681 tgaataaaaa cagaggttgt gaaatcagcg gtttgacctg agtattagag aaacaaacgg
    28741 caaaatagtt tttcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa
    28801 caccatgaat cagaggttgt aaaaaagccg tcctgcatcg agcaaaaaga atactcaaat
    28861 aaaattagct cctcatgttt tacatttgag aagactgatt gccgtagata aaagcttttg
    28921 aacaccattt atagaagaaa caagaatatt ttgctatttt aaacaagagg atagacaaat
    28981 acatgtctga tctcttgttt tttattgggc aacagacctc ttagaatgta taaagataaa
    29041 ttttacaaac gctttcggtt cagttataat aataaatgat aacttgtata tataagttgt
    29101 ggtaggtaat aaatcagttg aatggaggta aatatccatg acatttaaag acaaagtcat
    29161 ttaccaaatc tatcccaaat cttacaaaga cagtaatggt gatggaattg gagatttaaa
    29221 aggaatcaaa gaaaaactcc cgtatctgaa tgatctaggc atcgacatga tttggctaaa
    29281 ccctatttat ccaagccctc aaaaagacaa cggttatgat atctctgatt acacagccat
    29341 cgatcctatc tttggtacga tggaggaatt cgaacagcta atagccgaag caaagaaata
    29401 ccggatagaa atcatgctcg atatggtgct gaatcacgtt tcgacagaac atgaatggtt
    29461 caaaaaagca ctagcagggg accagcatta tcaggacttc ttcattttaa gagaagagcc
    29521 aacggattgg gtatcaaaat ttggtggaag tgcttggtcg ccatttggag atacggggaa
    29581 atattatttg cacttatatg atcggacaca agctgacttg aactggcgga accaagcagt
    29641 aagagaggaa ctattcaaag ttgttcgttt ttggatggaa aaaggggtaa aaggattccg
    29701 tttcgatgtt atcaatgtta tcggtaaaga cgaacaattg aaaaataata aagaaaatga
    29761 tggcaaacct gaatatacgg acaaaccaat cacccatacg tatctaaaag aattgaacca
    29821 agcaacgttt gggacggatt ccactatcat cacagtcggt gaaatgagct ctacgacgat
    29881 cgaaaattgt atcttgtata cagaacctag tagagaagaa ttatcaatgg tctttaattt
    29941 ccatcatttg aaagtcgatt atgaaaatgg gaataaatgg tctactcctc catttgattt
    30001 tgcagaatta aagcgactat ttcatacatg gggagaaaag atgagtgagg gtaacggctg
    30061 gaatgctcta ttttttaata atcatgatca gccacgtgcg ctcaatcgat ttgtagctat
    30121 cgatcagtat cgaaaacaag gagcagagat gctagcaacc atgatccatt tgaaccgtgg
    30181 gacgccctat atttatatgg gagaagaaat cggcatgatc gacccagact attcatcgat
    30241 ttccgactat attgacgtag aaagtttgaa tgcttataag ttactccttg aacaaggatt
    30301 cacagaaaaa gaagcgtttc gtcggatcaa agcaaaatct cgcgataact ctcgaacacc
    30361 catgcaatgg tcagacacag aacaagcagg atttactaca gggaaacctt ggttaaaagt
    30421 tggaggcaag ctagaagaga ttaatgtaga aaaagaacta tcttctgaag attctatttt
    30481 gtcctattac aagaaattga ttcggctgag aaaaacatat ccaatcgttg cacaaggaga
    30541 ttatcatgct tatgaagcaa atcatccgga agtttatggc tttcttcgcc aatttgaagg
    30601 acagcagctg cttgtattaa caaatttcta tgataaagat acaaggatcg ccatcccaga
    30661 aggattcaca gaagccaata ttcttatcga taattattcc gatactgttc ttcatcaaag
    30721 tctggagcta aaaccatacc aggcaattgc tttgctgaaa aattaaaaca aaaacacaaa
    30781 acgtcgttcc tacgagctat tctcgtaaga tcgacgtttt gtgttttatt taaaataatc
    30841 ttcttcagaa aatgaagagt tttcatactc tcctttatcg ccataaaatt tattgtagcg
    30901 ttgtttgaat ttacggaaaa taaaagtaac gagtaatttg actaacgtat agataggaat
    30961 accaaaaata acgcccacaa ttcccattag atcacccatg acaagtaaaa cgattaagat
    31021 cgtaatcgga tggatctgta aacgatcacc cattactcga ggaatcacca agtcaccgtg
    31081 gagaagttgt acggcaaacc agacaatgac aaatttgaca gccataaaag tagagtcttg
    31141 gaatgcaatg atcaaacctg gaaagaaggc gataaacggc ccaatatatg gaatgataca
    31201 taacaaccca gcagaaatag ctaaaacact agcaaattct aatccgatca gtaagaaaca
    31261 aacccagtag actgctccaa ggataaatga agcaatgatt tgccccttta gaaatgaacc
    31321 caattgttga ttagcaatct ttgtcaaatt tttaaaatct gtacgaaatg ctggtgggac
    31381 aatcgctaaa actgactgat aaaatttttg aggatctctc aaaaggaaaa aagcgacaat
    31441 tggaccagta aataaagtaa taaaaatagc tgtaacagtt gaaaaaatgt tgctgatccg
    31501 ttgtgcgcca ctttcccaat aatttccaat aaatccagct atatcagacg tcacgtcgtc
    31561 aatagaagcg aaaaattgat taaatgaatc ggcaaaaggt gttttctcca aaaattgtcg
    31621 gagactctct tgaaaatctt gaaacaaatc tggcaaacta ctgatcaatg tctttgcctg
    31681 ttcttcaaga aaaggaataa gccatatgct tccaagcccc aataaaaata aaataatcac
    31741 ataaacgata atgactgaca agttcctcga aaggtgcttg ctcaaacgtt tgatgactgg
    31801 ttccaaaaga taatacaaga tcagtgcaaa caataatggg ggcaagatag tagcgataat
    31861 gattccgaca ggcttgaata taaacgagat ctgatggaaa aaataaatga tcagcgcaag
    31921 taagataact attcctaaaa catagtaaga ggtttttcct cctaaaaagc ggatcaaacg
    31981 attcgttgta tctgtttctt ttttcactct ttttcctcct ttatctataa cttcattata
    32041 gacaaaagcg ggtataaaag gaaaatgata gaggttgatt gcaaaaatat cgtcaatcat
    32101 tcatataaac tttctcatca attaaaataa gaaaacgctt gacagttctt ttaaccatat
    32161 gataaattaa atcctgttgc taaaacgtaa ctattacggt ttgcgaaaag cgaatcggaa
    32221 cattcaaatg aaagaagatg agaagaggag tgtcataaat gaaacgattg ataggtatat
    32281 tggccatgct ggttttagca ggtatgttaa ctgcttgcgg ggcaagtggg aaagccgagg
    32341 acagtaaaga aaaattgagc gtgatgacga ccttttatcc catgtatgat tttacaaaag
    32401 caatcgtcgg ggatgaagga gaagtagagt tattgattcc agcagggact gattcccacg
    32461 attatgaacc ttctgctaaa gacatggcaa agatccaaga tacagatatc tttgtttata
    32521 atgatgaaaa catggaaaca tgggtccccg caatccaaaa aacattacaa gaaggcaatg
    32581 tccatacgat aaaagcgaca gaaggtatgc ttttacttcc gggaagtgaa gaaggacatg
    32641 atcatgacca tgaacacggg gaagaaggcc atacacatga gctagaccca catgtatggc
    32701 tagctccaag tttagcaatc aagcaggtag caaacatacg tgatcagctg attgaagctt
    32761 acccagaaaa acaggaagta tggacaaaaa atgcagcagc ctataccgag aaacttcaag
    32821 cactccatca gctttatcaa gaaaccttta agcaagcaaa acaaagatct tttgtcacgc
    32881 aacatgctgc attcaactat ctagcgttag aatatggatt gaatcaagta tcgattgctg
    32941 gtctttcttc tagtgaagaa ccgtctgcgg cgagaattgc tgaactgaaa cactttgtta
    33001 aagaacatgg aatcaactat atttattttg aagaaaacgc caaagatagt attgccagaa
    33061 cgttagcaaa cgaagcaggt gtatctttag aagtattgaa tcctttggaa ggattgacga
    33121 atgaacaaat agaaaatgga gaaaactatc tttctatcat ggaagcaaac ctcgaagcgt
    33181 tgaaaaaaac gacagagaca gaaaacccgc tagaagatac tttgaccccc aaaaaagaaa
    33241 agagcgtcta caacggttat tttgaagacg aggatgtaca agatcgctct ctttcagatt
    33301 gggcaggaac ttggcaaaca gtagatacgt atgtagaaga tgggactttt gatcctgttt
    33361 ttgaatataa agaaaaattg aatcaggata agacagcaca agaatataaa gaatattata
    33421 cgaagggcta tcaaacagat attgcttcaa tcaagatcag tgagaaagag atggcattta
    33481 cgtttaagaa tggaacaacg aaacgttcaa attatcgtta tgtagggaaa gaaatcttga
    33541 cctacgaggc aggaaaccgt ggcgtaaggt atctattcga agcagaagat aaggaaagtg
    33601 gcgcttttag atatattcaa ttcagtgatc acacgattgc accggctgac tccgaacatt
    33661 ttcatttata tgcaggaaat gaaagtcaag aagtattgct gaaagaaatg gagaattggc
    33721 caacatttta tccagataca atggatggac gtgaaattgc acaggaaatg cttttgcact
    33781 gataggcaaa aaagaaccga agggtttgga tcggctggtc atttagtcgg tccaaactat
    33841 tcggttcttt ttatgaaatc aatttcttta atccacggat agaggctggg atggtctcgc
    33901 cgtttgccaa cgtgacttct ctggtttttt tattaatcga ccgcacattt tgtgtattga
    33961 taacatagga tttatgacaa cggataaaat tccgatgcat tttctcgatt tctttgatct
    34021 tctcatagaa ttgtaattgg gcatgagtca aatggacttc cagtttatgt ggcgctccta
    34081 gcgaagtaga aaaatattgg atgtctttga tcggtaaaaa tttgatctca tcattaaact
    34141 ctattttgaa gatatctttt tggatttggg acatttgaaa atcttcgaaa attttgctca
    34201 ggatatgctc gacattttct tttaaatcat aaagatcttc tttgacgata taatcagtcg
    34261 gttcgatgtt gctttttaag attttcatcg acatttccgt atgggaagtc acatagatta
    34321 tttttcctaa aggatctaat tcacggatca atttaccgat cgctacacca tccatttcac
    34381 tgtcttctag ttcgatatct aaaaagtaaa taccaaaaga agaagttctg atcttggctg
    34441 cattgagaag ttcttccgga tcagaggtag aaatatggac ataactgtct aagttttcaa
    34501 tcatgattct atttttgatc accgttgata aaacttctaa ttgttttgga tcgtcttcac
    34561 aaatataaat gggaatcata tcattactcc tttattttat ttgtatataa atcgtttgag
    34621 aaaaacgctg gttttcaatt gatgtttcaa ggaaaagata aggattggtc tttgtcaatt
    34681 catccaagat aaacaagcca aggccttcgt tttttggatt tgtttttgtt gagaaccctt
    34741 tttgtttcaa ctggtttaac ggtaatgtat ttgttgttgt attgatcaca cgaatcatca
    34801 cggaatgatt cttcttgagt atggtcaatt cgattttttt cttctttgca gcactagctc
    34861 cttcaatcgc attgtccaag ataataccaa gcatacgtac aaggtttacc gtatgtttcg
    34921 tatccagttc gattggctca ggaaaatgaa gctggacgat caaccctttt tcctgtgcca
    34981 tcatgagttt taatgacagt aaactacgga tctcaggaac tttcatatta tccaacatac
    35041 ttagattggc cggaaaaagg ttcatcatct ggcgtgtagg taatatcgtt tgatcataat
    35101 aagaccgtaa tccttctaag tcgccttctg aaatatagct gtgaagagaa aaaagcagat
    35161 tttggtaatc atgtttgaat tctctcaagt cttgatagtt tttttctaga tctttgatat
    35221 aatcctgttg catcctgact tgttgctcta ataactgatc agctaatttc ttattagaga
    35281 ttttccaata ataaaccaag atgatcaggc caacacacag atatagaatg aaaaaactcg
    35341 tattcaaagc taagacatca ggtgtttcac cagaaaaacg ggtataaaaa ataatgatat
    35401 agtatgtaaa taatatcaat gtcccgatcg taccgatcat acggcggtcc acagtgtgaa
    35461 accaagtctc taacgatttt tttaaacaaa aagctaataa agcagataaa aaagcggata
    35521 agacaatgtg aaaatactgc aatgaatctc ctggttcaat aaccgaagat ttcaaaacaa
    35581 aaaaatcgcc gatgcttgtt aagtgatctc ctaaaagcga cgtcaataac ccaaaaggaa
    35641 gcgcgtaaac caattgttga cgcgaagtcg aaaagaagaa aaaagtaagc atcagcattc
    35701 ctatagcaat tagagcagta aaaatcccta aaatcgaata gacccatcca atcaaaatac
    35761 aagaaatcag aatgactccc gcaaacagat atttgtatcg gatgtgatgg tcaatcaata
    35821 agatacaaat tgtgtaaatc aaatattgca acgctatgga ccataaaaac ataaagtaca
    35881 gctcccattg atttttacca aattatagca caattggcgt attatttttt taatttttca
    35941 gggattttga attcagaact cataccaaag cagcagccag atacaaaaaa gtcagataag
    36001 ttagttaatt gtttaatgat tgtttttttc atgaaagatt cctccaaaat tcatcaagta
    36061 gtttgtaata ggaagcaacg tagctatttc taaaacaatc ccaagtagca aataggtaga
    36121 aaagtaagaa gctgagaaaa ataacgtaat gagagaaaaa attagaaaca tgctgatcgc
    36181 cttctttaat aatgggggac gtttctcggg ttcaatggga ttgattgctg tacctcttga
    36241 tccatagcga agcagtaaca aaaagcagct gattaacaaa aagaccatca ttgggaagga
    36301 gatagtcaat tgcagatgtt tcatcaccaa gggaacgccc cataaataaa gtagtgtcca
    36361 cagcagacaa acgaattcag agtcggaatg caatccataa gcatatctgc ggatgcagat
    36421 ataagaaaga aaggtgattc ctgtttccgc ccatgagcct gtcaccagag aaaagactag
    36481 aacgaccgct agttttccaa gattattcaa taaaacttct aagacaaatt ttacttggac
    36541 atacaccata tcttcttccg atttgtctcc gctgaataag gcttctaata atttgttcac
    36601 aactttatct ttcatctcat acgtacctcc accacaaatt ttatgctaaa aaaaataaaa
    36661 aacgtctttt gtttcataaa aagtggtttt gacatcaaaa ccgtcatgat ctcctaaaaa
    36721 aatcttcgtt ttccaagaac cttaccaaaa aaagtgacag ttttgatacg aaaagtactt
    36781 tttatgacaa gtttgtgctt tttcgagtga tttatgttaa aaaattactg accttataaa
    36841 aggtgatgga gggattctta tggaaaatga acaattgttt gaagtcggcg aaacatatgc
    36901 atttaaatct gatttatttg aatcaccaat aaaaggaaaa atagaaaaaa tctatgaaaa
    36961 tagtgtgatg atacgtgtca tgaaatgcgc agtagaagac aatgataccg ctcataatct
    37021 gcactggcag ttagttgtac gcaaatcaag tacaatagaa gaataaaaac agataaaatg
    37081 ttgagagcat aaagaggttg tgagtctccc gtttagctct gagttacaag gaataatgtt
    37141 aaaaaacagc ttttcatgtt ttatgaatat tgccttgtga ccgaggtgtt ggcaggagaa
    37201 tactgtttat ttgaaaaaag ggctgcgaca agacttttgt cacagccttt attttcatga
    37261 ataggagtta cttttggcta cttttagaga agtagctttt aaggagcaga taaagtcaag
    37321 gcttttattt ctataaatga agtagtaaaa tgaagaacag attttattac aggaaggatg
    37381 ccgataattg tgatcattgt tgcaggaacg atcggtgcag ggaaaagcac actaactgaa
    37441 atgcttgccc aagatttaga aacaaaacct ttttatgaga acgtggaaga taatgaagta
    37501 ttgccgctat tttattctaa tccagaaaag tatacttttt tattacagat tttctttttg
    37561 aacaaacgct ttttggcaat caaagatgct ttcagccatg atgacaacgt actagaccgc
    37621 tccatctacg aagatagtat gctgtttcat ttgaatgcag atttagggcg tgtttctgaa
    37681 gtagaagtaa agcagtatga aggtctttta gaaacgatgc tgaaggagtt agaagaaatt
    37741 tcgccacaaa agaaaccaga tctactggtt tatattcgtg tatcctttga aactatgtta
    37801 gcgagaatca aaaaaagagg ccgggaatat gaacagctag aacaagatcc tgaactctat
    37861 tcctactata aagaactcaa tcgacgatac gaagagtggt atgaacagtt tgatatatgt
    37921 cccaaaatag tgatcgatgg ggacaaatat gatttcgtag ccgatccggc atgtggagaa
    37981 caaatcgtac aagaaatcaa gatgcgggca aagaaaatga cagaagaggc agatgcccag
    38041 tcgatccgct acactacaaa taacccgaaa gcaaagggaa tctttacaac taaataatat
    38101 aaaaactaag caaattgctc actcgcagat atttctctgt gagcagcatc ttgcttagtt
    38161 tttttgattc attgagatca aatagtgaac cgattcgtat gataaaattg ccataaatga
    38221 aatgtctgag tgagggccat gatcaaaaat aaaatggaaa cgatcaaaat cgaatgggcg
    38281 ccaaacagat gatgacagaa aacaaggaca atagctcctg cgataaaggc aagaacgaca
    38341 cttagatagt ggaaaaatcg atcaaaatct gctttttctt tagaaataca ataatcatat
    38401 aagttcgttg ctaccccttt taaatttcca gttgtaaata aattagtgta ggcacgcccg
    38461 ttgattttat caaaagctac ccactggatc gatgcagtaa aagcaatcaa aatcgttact
    38521 aatgcgggat gctcatggac gggtgtcaaa ctaacaaaga aaaaaatcac tgcttcataa
    38581 tatagaacaa acaagcgcca gaaatgtttt tctttttttc taaaataatc aattagatac
    38641 tttgttagaa gaatccctaa gaaaaagaaa agggtagaca atactttttt accaacactt
    38701 agccattctc cgtcaaatgc ttggatgatc gatagaatga tattgccagt ttgagcagag
    38761 gcaaaagcac catattggat ataagtataa ctgtccatgg ctcctccgat aaatgtcagc
    38821 aaaagtccta ttgtacgccc ctcgtgaaag gaagtctgca tttttttcat caccttttat
    38881 gaatattttt gtcttcctta tcttagcata aacgattgaa aaaaaggaaa aattttcaga
    38941 aaactaaaat catatcgctt cccattttca tttttagaaa ggtctgttag tataaagaaa
    39001 aaatagacaa cgaaacttta aataaccaac ttgaaataaa caaattcaaa gaagaaagat
    39061 gggaaggaaa tcaatcagtt gaatgaaaaa gaaattctcc gattacaaaa agaattgaaa
    39121 cgctgggaga acaagtataa aaagcttcaa caagtctttc ggctacaaga agaaatcatt
    39181 gctacctatg aagcggctta tggcgaagca cccggactta ttgaggaaga agtacaagta
    39241 aaagaaagtg aaggaaacag ctctttcttt aaaatgatgg agaaacgttc ctataatcgc
    39301 ttcaatcaag aacaaaaagc agctatcaca tatgatatga agaaacatct tcgaatcata
    39361 gcaggagctg ggagcggcaa aacgcaaaca atctgtgcaa aagctgttta tctgatgacg
    39421 caaaaacaag tagacgaaga acgcatacta atgatcactt ttacaaggaa cgctgcaaat
    39481 gaactaaaaa aacgagtaga taatttcagc cagagaaaaa ccgctgtcca tatcggaaca
    39541 ttccatagta tcttttttag gatatacaat gaaatttgtc gaaaatttcc tgaagtagca
    39601 atgcaaggaa tacaaggaga cttttcaaag gatactgcac aaaaagtgaa tgctgttttg
    39661 caacagctga tacgtaaata taatctttat atatttgatc aatatggaga aaaaacgatt
    39721 gcttctcggt tagactattg gcagaatatg aacctttcag tagaagaaat ggttcagtta
    39781 gttaaagaaa aatatgattc cattgataag aatgcacgac agccgatatc agaacgactg
    39841 tatggattgc tcacggaact ccaagaacaa aaaagaagcc agcaattgct agagttcaat
    39901 gatgtactcc aaaatctgaa aagcgctttg gagaatgagg agatccagca ttatatcggc
    39961 caaaaatatg attatctatt cattgatgaa tttcaagata ctaatccact tcaatgggaa
    40021 atcgtgaaac ggatgacaaa ggaaaacagg atcaagttga ttgttgttgg agatgatgat
    40081 cagagtatct atggattcag aggatcagag ccagcataca tcaagaactt tgaaaaagaa
    40141 tatcctacca aaactctttt cttattaact aattatcgtt ctcgggcagc tattgtccaa
    40201 ggagcaaata gattgatcag ctataataaa aatgatcgga taccaaaggc gatgattcca
    40261 gcacaaaaag aagcaggtgt tatggaagcc tatttgtttt ctgatactaa agcagaaagt
    40321 cagtggctaa tcaaacaaat tcagacattt attcaaaaaa atgggaaata taaggaatca
    40381 atcatattat accgatcagc ttcacaaacg cagcagcttg tccaatctct gctgaaaacg
    40441 aatattcctt ttgtactgga agcagatagt ccatatgaag gtatctttgg tatcaagtct
    40501 ttccaacatt tctatcaaaa aattgttcat tggcaaacag cactgaatct gcaaaagaaa
    40561 aatcaggcct accagcaatt gctccggcag ctgatggcag actgctacct gaaaaaaagt
    40621 gaatgcgatc aatacttttc tcctaaatat caaaatatag ccgttgcaga ttatattttg
    40681 tcgataagcc ccaatttaaa atcgaaatct gcagaaatcc gagagtttga ggaagcgttg
    40741 cgtaaaacag accagcatca agaaatctat tcgcttgttc aaagctatct tcatctgcca
    40801 cgtatagcta aggaaatcga ccagagtgat caagaatgga tacgagaaga agtaaagcag
    40861 catgctactt tcagaagact ccaagcctta tcgcaagaaa cccaagcgac agctaaagaa
    40921 ttaaaaaccc gcttgattgc ttacagacaa ggaaaacttg atgcactatg tatccagagc
    40981 attcacaaaa gtaaaggtct tagttaccga aatgtctttt tgattggttg taacgaagga
    41041 agtttgcctt ataacggagc gacagaaaag aaagtaatcg actttaatga aataaaagca
    41101 gaaccagtaa cgacgataga agaagaacga agactatttt atgttgcgat gactagagca
    41161 agaaatcgtc tgtatctatg tgtacctcaa atgaaaaatt caagaaaatt gaaagtatcg
    41221 agatttatca aagaaaccgg gagtcgtgtg aaaaaaggaa agtgatagaa gagagggtgt
    41281 gacagaagtg gtcagtttca agaaagaaga aggaatgtac gaaaattgct tctcaaattt
    41341 ttgtgaaatt tcagcttatt ttcgaagaag ctatttctgg aacacagttt attcggaaaa
    41401 gggaggtgtg acaagaattt tgtcacacct cccttattta ttttgaaaat gagaatacta
    41461 ttatcaaaat agagaatata tagaaaaatt ctcattattc aaacgtaaat aatcagttat
    41521 tttgattttt t
//
LOCUS       DACDYM010000018        40612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDYM010000018 DACDYM010000000
VERSION     DACDYM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.018"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            336..1439
                     /locus_tag="IWM59_000967"
     CDS             336..1439
                     /locus_tag="IWM59_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9979015.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="IWM59_000968"
     CDS             1723..2403
                     /locus_tag="IWM59_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9979016.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="IWM59_000969"
     CDS             2493..3527
                     /locus_tag="IWM59_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9979017.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="IWM59_000970"
     CDS             3541..3876
                     /locus_tag="IWM59_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979018.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="IWM59_000971"
     CDS             3860..6340
                     /locus_tag="IWM59_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979019.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRVEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="IWM59_000972"
     CDS             6396..7955
                     /locus_tag="IWM59_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9979020.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="IWM59_000973"
     CDS             7952..9316
                     /locus_tag="IWM59_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9979021.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="IWM59_000974"
     CDS             9487..10557
                     /locus_tag="IWM59_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979022.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="IWM59_000975"
     CDS             10572..11240
                     /locus_tag="IWM59_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979023.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="IWM59_000976"
     CDS             11260..11838
                     /locus_tag="IWM59_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9979024.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="IWM59_000977"
     CDS             12241..13152
                     /locus_tag="IWM59_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9979025.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /locus_tag="IWM59_000978"
     CDS             complement(13408..14325)
                     /locus_tag="IWM59_000978"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9979026.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="IWM59_000979"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="IWM59_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9979027.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15552
                     /locus_tag="IWM59_000980"
     CDS             15022..15552
                     /locus_tag="IWM59_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP9979028.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            15684..18044
                     /locus_tag="IWM59_000981"
     CDS             15684..18044
                     /locus_tag="IWM59_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9979029.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18131..18445
                     /gene="trxA"
                     /locus_tag="IWM59_000982"
     CDS             18131..18445
                     /gene="trxA"
                     /locus_tag="IWM59_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9979030.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18627..20405
                     /gene="uvrC"
                     /locus_tag="IWM59_000983"
     CDS             18627..20405
                     /gene="uvrC"
                     /locus_tag="IWM59_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9979031.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20550..21449
                     /locus_tag="IWM59_000984"
     CDS             20550..21449
                     /locus_tag="IWM59_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979032.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21442..22704
                     /locus_tag="IWM59_000985"
     CDS             21442..22704
                     /locus_tag="IWM59_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979033.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22867..24765
                     /locus_tag="IWM59_000986"
     CDS             22867..24765
                     /locus_tag="IWM59_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9979034.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25064..25783)
                     /locus_tag="IWM59_000987"
     CDS             complement(25064..25783)
                     /locus_tag="IWM59_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9979035.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26120..26452
                     /locus_tag="IWM59_000988"
     CDS             26120..26452
                     /locus_tag="IWM59_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979036.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26439..28442
                     /locus_tag="IWM59_000989"
     CDS             26439..28442
                     /locus_tag="IWM59_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9979037.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28461..28931
                     /locus_tag="IWM59_000990"
     CDS             28461..28931
                     /locus_tag="IWM59_000990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9979038.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28952..29536
                     /locus_tag="IWM59_000991"
     CDS             28952..29536
                     /locus_tag="IWM59_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9979039.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29551..30537
                     /locus_tag="IWM59_000992"
     CDS             29551..30537
                     /locus_tag="IWM59_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9979040.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30527..30838
                     /locus_tag="IWM59_000993"
     CDS             30527..30838
                     /locus_tag="IWM59_000993"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9979041.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30860..32641
                     /locus_tag="IWM59_000994"
     CDS             30860..32641
                     /locus_tag="IWM59_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9979042.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32634..34010
                     /locus_tag="IWM59_000995"
     CDS             32634..34010
                     /locus_tag="IWM59_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9979043.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34017..34652
                     /locus_tag="IWM59_000996"
     CDS             34017..34652
                     /locus_tag="IWM59_000996"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9979044.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34682..36037
                     /locus_tag="IWM59_000997"
     CDS             34682..36037
                     /locus_tag="IWM59_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9979045.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36229..37623)
                     /locus_tag="IWM59_000998"
     CDS             complement(36229..37623)
                     /locus_tag="IWM59_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9979046.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37715..38686
                     /locus_tag="IWM59_000999"
     CDS             37715..38686
                     /locus_tag="IWM59_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9979047.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38683..39465
                     /locus_tag="IWM59_001000"
     CDS             38683..39465
                     /locus_tag="IWM59_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9979048.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39634..40005)
                     /locus_tag="IWM59_001001"
     CDS             complement(39634..40005)
                     /locus_tag="IWM59_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9979049.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40139..40582
                     /locus_tag="IWM59_001002"
     CDS             40139..40582
                     /locus_tag="IWM59_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9979050.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
BASE COUNT        13420 a         6917 c         8648 g        11627 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gagtcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa tcaattcggc
    15481 gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa caagatgtat
    15541 gagctttggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt
    15601 tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc
    15661 atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa
    15721 aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag
    15781 aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg
    15841 gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaacttgaa aatatccgtc
    15901 cccatatgaa acggatcgag atcggagcag acttgaatgg agtggagctt gcacaagttt
    15961 ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg
    16021 aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga
    16081 gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg
    16141 agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag
    16201 atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa
    16261 atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc
    16321 acgatcaaag tgcttcagga cagactttat ttattgaacc aaaacaagtg gtggatttga
    16381 ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg
    16441 aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct tatgtgattg
    16501 gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc
    16561 cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc
    16621 aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta
    16681 caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga
    16741 tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg
    16801 aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt
    16861 ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat
    16921 ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct
    16981 tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga
    17041 aagtttatgg atacaatcgg gcaaatacga ttaatgccag catggagttc gatgtggata
    17101 cactaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa
    17161 tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg
    17221 atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag
    17281 agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt
    17341 tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga
    17401 aaaaagcaaa cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata
    17461 ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg
    17521 caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga
    17581 agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg
    17641 gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat
    17701 taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac
    17761 aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc
    17821 ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca
    17881 ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca
    17941 agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg
    18001 caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa
    18061 gcagatggtt cgctatccaa gtatagaaat gctatactaa tcatagattt taaaaatgga
    18121 ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg
    18181 acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat
    18241 tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt
    18301 ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt
    18361 gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg
    18421 tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg
    18481 aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt
    18541 ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt
    18601 taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac
    18661 ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg
    18721 gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggcagc cataatacga
    18781 aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata
    18841 tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta
    18901 tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt
    18961 tggtgattac tcgtaaagta ctaaaagaca aagcactgta ttttggacca tatcccgatg
    19021 tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca
    19081 aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgt ctgtgtcctt
    19141 attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt
    19201 taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg
    19261 aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa
    19321 tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg
    19381 cgattgacaa aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg
    19441 aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc
    19501 tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata
    19561 cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag
    19621 gagaaaagaa aaaattagtt gaattggcga ataagaatgc ctcagttgct ttgaatgaac
    19681 ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa
    19741 acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg
    19801 ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat
    19861 atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag
    19921 tcatctatcg cagatattca cgagtgattc gcgaagggtt gcctttacct gatttgatct
    19981 tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag
    20041 gaatcgatat tcctattgct ggcatggcaa aaaacgataa acataaaaca aatgagttgc
    20101 tattcggtat agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac
    20161 tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca
    20221 gtaaaaacag ttttgcttcc cgattagatg aaattgaagg gttaggacca aaacgaaaaa
    20281 aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat
    20341 tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg
    20401 aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa
    20461 aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat
    20521 atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt
    20581 ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg
    20641 ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt
    20701 tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg
    20761 atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc
    20821 aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg
    20881 atttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt
    20941 ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga
    21001 tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg
    21061 gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata
    21121 atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg
    21181 gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt
    21241 taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca
    21301 atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga
    21361 ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca
    21421 aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta
    21481 aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt
    21541 atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta
    21601 ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag
    21661 ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat
    21721 gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat
    21781 tcattaggac aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg
    21841 cttcgagcaa gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca
    21901 agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt tggagaagat
    21961 aactcgacag tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt
    22021 ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg
    22081 gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaact aacaggtgtt
    22141 ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt
    22201 caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg
    22261 attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct
    22321 gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt
    22381 ttgccagtga cttatctatc tctaggcgga tatatgcttg gattgatact aggagcttca
    22441 gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt
    22501 atgccggttc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg
    22561 gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat
    22621 gtactcgtgt atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg
    22681 agaaatgaac gaaaaaaagc ataaaatata gaaaaatata gaaggaagct ggacatttat
    22741 tgttcccagc ttcttttttg tatagaccta tttctatttt tttattatat tggatatata
    22801 tattcattga aaatagagca taacaacttt ataatgttga gtataccaat ttttggaggc
    22861 gttttaatgg accgctattt agatttactt tcagagaagt tccctacaac ggaagatgtc
    22921 atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt
    22981 ttgagcgata tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga
    23041 aatcttaaag aaaagatcaa agaccttttc tctgaacaaa tgacggaaac ggagatcagc
    23101 caattcacta tttttacggc ttatccagaa tatgtgttga cgactgattg gtacaaaaaa
    23161 caagataaat cccagctgat ccatcgtttg atcgatcttt tacggtttac gacagtcaaa
    23221 tacactcgat caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa
    23281 ctcttgtata tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa
    23341 caacttgtct tgatgaagga agaagaaaaa tttttaacaa aacttgcgca gacgatccaa
    23401 aaatctgtca tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg aactcaagca
    23461 gccaaagtaa tggatcgaat catggatttt tcctctgtag atatccagtg ggggaatcat
    23521 gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttattgac gcttttacgg
    23581 attgccgcac gctacaacta tttatttgaa ttagagaaag aatatggatt aaatttacgt
    23641 ccattatttt cttttgccca tcaaacctat cagaagaatc cagtatttac ccctaaaaac
    23701 aggaaagatc tatctgaaag agagcaagaa gaattagaaa agatccatca agcactttct
    23761 atcctccaat tcaaatcaga acatcagttg ttgaatcgac gacctgaatt taaaatggac
    23821 gatcgtaaat tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt agaaggtcag
    23881 ctctatgagt tgaaagggac ttgttttcaa acgatccagc cagatgatcc taaaaaattc
    23941 ttggaagagg aacaaaaagt gatcgatagt ttgatgtatt cattccagca ctcgttaaga
    24001 atgcaaaaac acatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag
    24061 catgtactgt tccatggctg tatacctgta gatgaaaagg ggcagctctt agcatttgaa
    24121 ttagaccaat cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca gataactgaa
    24181 agcgcaaaag atttgactgc aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg
    24241 agcgggccat tttcgccttt attcggcaag agtaaaatgg caacttttga gcgctatttt
    24301 ctcacagagc ctcatacgca cgtcgaaaaa agcaatccct attatcactt gcgagatgaa
    24361 gagtggttca gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc
    24421 atcaatggac acacgccagt aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga
    24481 aaagcatttg ttattgacgg aggtctatcc aaagcttatc aaaaaacaac cgggattgct
    24541 ggctactcat tactatgcaa ttcttacggt ttccaaatcg tgacacatta tccgttcttg
    24601 cctattgaac aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag
    24661 cagcagctcc cacgcaaact taatcggaat acgacaaaag gaatggaatt acagcaacag
    24721 attactcaac tccaaaggct gttggattat cgaaaggacg attgagaaaa atgctagcca
    24781 acattgctca atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga
    24841 attagctcaa aaaacggtaa aatattagaa gctatttttc tgtttatttc tctaatactc
    24901 aggtcagggc gctgatttca cgacctcttc cttaaacggt gttctcctgc caactcctcg
    24961 gtcacaagtc acaatatcca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    25021 ctcagagttg aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa
    25081 agaaatcgaa taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt
    25141 ggacaccaag gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt
    25201 tggaaaaggc gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg
    25261 aattcacaaa gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga
    25321 cttcgtgagt tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg
    25381 gatataatga aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc
    25441 ctcggcagac tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt
    25501 cgaattggtc tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa
    25561 gttgcggact aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt
    25621 gaggcgctga atctggagac atgactggca gaataagagg taatccttga gcatctaaaa
    25681 ctgcatccac aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg
    25741 ctcgaatcag ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag
    25801 tagactaatt gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact
    25861 tatctgttct tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat
    25921 agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc
    25981 gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg
    26041 cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc
    26101 ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc
    26161 agaagaagaa aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga
    26221 agaaaaagag caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt
    26281 ggtagaagaa aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa
    26341 agcagaagca gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca
    26401 agcaatcggt gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa
    26461 aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa
    26521 tccaaaatgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa
    26581 aatattttcc agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc
    26641 ggctgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa
    26701 agaaactcca tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg
    26761 aagaaaattt catgaataaa ttagaagaag tccttgattt aaaagaaaag tgggaacagc
    26821 taactgaaaa acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc
    26881 tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga
    26941 gtgcagcaaa ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg
    27001 aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag
    27061 ttgctgctat agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg
    27121 aacaaaatcc taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc
    27181 agcaaaaaaa gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat
    27241 gggtagaaga agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc
    27301 atgccactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga
    27361 tgcatgtgtt aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa
    27421 actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac
    27481 cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc
    27541 cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg
    27601 gattgctgat gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga
    27661 tgaaacgttt cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga
    27721 tctacagttc atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt
    27781 tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg
    27841 gtctgataca gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag
    27901 attacttgag tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta
    27961 ttgcattgat tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc
    28021 tatcgatcat tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag
    28081 tcaagggtgc tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt
    28141 tagtcagtta tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat
    28201 tcaatatgct agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga
    28261 tcatcgcttt acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg
    28321 cacgattgca gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg
    28381 acccgctgaa aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat
    28441 aattggagga tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat
    28501 ttgcagtatt agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg
    28561 ttggaatgac aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac
    28621 aggctttgat tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct
    28681 ttttgatttt tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag
    28741 gcgcttctct gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg
    28801 cagcagcagg tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt
    28861 ttgctgcgat ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat
    28921 tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca
    28981 gatgaatgaa gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat
    29041 cgatcaaaac tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga
    29101 aaaacaaatc gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga
    29161 agtacgacaa gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc
    29221 aatagctgaa atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt
    29281 gtattcatta cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta
    29341 tctttctcag acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag
    29401 tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa
    29461 ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa
    29521 tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt
    29581 cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat
    29641 tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac
    29701 ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg
    29761 ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc
    29821 cataatctga aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat
    29881 atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca
    29941 aatgaactcc ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag
    30001 tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga
    30061 cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac
    30121 ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg
    30181 acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat
    30241 aaagatatgg aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc
    30301 cctgtcatcc gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat
    30361 ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc
    30421 tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga
    30481 agccatttcg aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat
    30541 cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg
    30601 ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta
    30661 tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta
    30721 caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat
    30781 agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta
    30841 tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt
    30901 aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg
    30961 agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga
    31021 gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga
    31081 attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt
    31141 tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca
    31201 tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga
    31261 tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg
    31321 gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg
    31381 tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag
    31441 acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg
    31501 agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt
    31561 tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt
    31621 tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc
    31681 tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa
    31741 tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc
    31801 ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg
    31861 ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta
    31921 tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc
    31981 attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg
    32041 aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg
    32101 tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta
    32161 ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc
    32221 atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt
    32281 gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa
    32341 atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc
    32401 aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg
    32461 tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga
    32521 aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat
    32581 tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta
    32641 aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg
    32701 gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac
    32761 aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta
    32821 tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg
    32881 aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga
    32941 tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt
    33001 atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc
    33061 gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc
    33121 agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg
    33181 ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg
    33241 caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg
    33301 ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg
    33361 ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag
    33421 cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg aacttagcta
    33481 ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga
    33541 ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta
    33601 cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg
    33661 atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag
    33721 aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa
    33781 aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga
    33841 agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga
    33901 ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa
    33961 aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg
    34021 cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa
    34081 ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca
    34141 tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag
    34201 caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat
    34261 ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa
    34321 aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg
    34381 ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc
    34441 caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg
    34501 aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa
    34561 cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga
    34621 tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac
    34681 gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt
    34741 aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa
    34801 tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt
    34861 aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat
    34921 catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc
    34981 attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct
    35041 agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgttat
    35101 ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa
    35161 aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt
    35221 gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc
    35281 tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca
    35341 gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt
    35401 aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa
    35461 tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata
    35521 ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat
    35581 gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt
    35641 gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg
    35701 taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt
    35761 gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat
    35821 cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt
    35881 gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg
    35941 agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa
    36001 atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta
    36061 gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc
    36121 aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta gtgcctcttt
    36181 tttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata
    36241 tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga
    36301 ggatagggaa attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg
    36361 ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttagggt caatctcagg
    36421 atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag
    36481 agaatcaaaa agctgattgg ctctttttgt aagacttaat ttagtttcta agatcctatt
    36541 tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac
    36601 atttatatta aaaattgctt gttctatctt gctttggaca tgtactggaa ctacaagata
    36661 agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt
    36721 aggtaataaa ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga
    36781 atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc
    36841 tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac
    36901 accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc
    36961 gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc
    37021 gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat
    37081 agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc
    37141 ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg
    37201 gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac
    37261 gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac
    37321 ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc
    37381 aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt
    37441 aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg
    37501 taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg
    37561 cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg
    37621 cattttctcc actccttaaa attgtatacc atacaatttt accattgatt gtattgattt
    37681 tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt
    37741 agtagcttct tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta
    37801 tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaagg
    37861 caaaaatatg tttgcgatga tgcctgctta tttgggggag aacagatatt ttggcacaaa
    37921 agtcattacc gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat
    37981 tttcttgttt gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac
    38041 ctggattcga acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc
    38101 agaaacactt gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc
    38161 taccattcgt cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac
    38221 ttttatcgct aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt
    38281 agaagatgca gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt
    38341 tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc
    38401 tgatacaaga gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta
    38461 ctcagctgcc ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga
    38521 ggaatcttgt attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa
    38581 aggagaatcg aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg
    38641 tgcagaatat atttataaca aattagagaa aggggcttta aaatgaactt agattattac
    38701 gtagttgatg catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta
    38761 gaagaatggc tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa
    38821 acagcattta cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga
    38881 gaaatcgatc tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac
    38941 aaaatacctg atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga
    39001 attaaagatt attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg
    39061 tctgaatatg aagaagcgat tggggctaag attaaagaag cctacttagc acgtgattta
    39121 tttttcgtat tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc
    39181 aaaaattttg aattaggaat aggagtcatc gtgacagcag aaagtgtgca aaaagacttt
    39241 gtttctagaa ccttctttcc aaaattaaca atcaatgaag atccagtatg tggatcagca
    39301 cattctaatc ttattcctta ttgggcagaa agaatgaata aaacaaaatt gtcagcttat
    39361 caaatgtcat caaggggcgg ggatctagaa tgtgaattaa aagaagatcg tgtgataata
    39421 ggaggaaaag cagttttgtt ttctaaaggt acctcgttta tttgatttct ttcaaatgaa
    39481 ttaaaaataa attggtggag agtggcaacc ttatagctac aatggttgtt aaaaaaagac
    39541 gcaaactttt tcaatttaat gaaaagtttg cgtctcattt taattattgg tgtttgtttt
    39601 ttattcaatc aagagtaaaa tgccccatac taactatcct cggatgactt cgattttata
    39661 accatcaggg tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc
    39721 agtaatctca agaccggcag ctttatgttg ctcgtgtaat ttttctagat catcagctgc
    39781 tatagcgata tgaccatagc cgtctcctag atcatacgca ccatgatcat agttgtaagt
    39841 caactctaat tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa atttgttttc
    39901 tggaaaatca cggcggcggc tttcttcaaa accaaatgcc ttttgataaa attcgattga
    39961 tgcttcaaga tctttcactc gaacacatgt gtgagccatt ttcatagatt catctctcct
    40021 tttcctttta ctcaatcata acaaaaagaa gtaaggaaat agttgatttg tgcttgttca
    40081 tgagaaattt ctcatgtaaa atggatagaa aataaaactg ttaaggtgga aactattcat
    40141 ggataaacca atttttggtg aaaaaaagca atttgctgat tatcaaaccc gttatgcagc
    40201 atatattgtc atagaaagag atcagcagat tgcagtaatc gaggctccaa atggtgcttt
    40261 ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga
    40321 gctgatagaa gaaatgggaa ttgtggcaga aatttcttct tatctgggag aagctgatga
    40381 gtatttttac tcgaattatc gtgcaactta ttattataat ccaggatatt tctttgttgc
    40441 tgattcttgg aagcgtatag gagaaccaac tgaaaagacc aataaaattt ggtgggtgag
    40501 ccctcaagaa gcgcttgtga aattgaaacg tggtagccac cgatgggcag tcgagagatg
    40561 gatcgaaaaa aataaaaact gaaagaaagc gagtagatca gctttctctc ag
//
LOCUS       DACDYM010000019        39581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDYM010000019 DACDYM010000000
VERSION     DACDYM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.019"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2123)
                     /locus_tag="IWM59_001003"
     CDS             complement(<1..2123)
                     /locus_tag="IWM59_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979051.1"
                     /translation="MKIIKKKIDNRKINWLLISFLILIAMIIFTSNVAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQSVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMINKT
                     NDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVATKFSDN
                     FLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLNAGEGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERRLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTMSAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENPQNSTSSQDRRSVDVREN"
     gene            complement(2110..2328)
                     /locus_tag="IWM59_001004"
     CDS             complement(2110..2328)
                     /locus_tag="IWM59_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979052.1"
                     /translation="MIENHLVDKIYCLDHNADTLELIESICSNTIVLDLRAVNDDYVT
                     HIIVNKKMAQKLSESLKKWAEGGNNENN"
     gene            complement(2321..3061)
                     /locus_tag="IWM59_001005"
     CDS             complement(2321..3061)
                     /locus_tag="IWM59_001005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979053.1"
                     /translation="MREKLKKLGDKKRHTFQGTFEFNGYKKDKYKFGQEYENATFMLS
                     NLKIVQGDNLELVTDHIWMNLTKQFLKFGWLKKGDMVQFDGRIKSYSSKKGINYKIER
                     PSKVKVFRKGTEINESSALKISTRYEIVEDIKRQNREYYEARDFFFENYFSVPRYFKL
                     KEKWPEIQAVVAINENRYTSEQFKQVFKDRDSLVDLYKKIQETDKYNLQKEFYNGLVS
                     NKNRIDLNEVEGYLHKIENFDYGRGYFD"
     gene            complement(3194..4078)
                     /locus_tag="IWM59_001006"
     CDS             complement(3194..4078)
                     /locus_tag="IWM59_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HAP9979054.1"
                     /translation="MIFMAEKRMLAKSFLNNASFMKMSDSAKLLYVYLNTNADDDGIV
                     EAYPYTKMLGTPDDDVKILFTKGFVFFLNEDLLAFLPHWLQNQNIRIDRYKPSIYRFK
                     LLNLKPEFFSKLKPIKFDMILQSGQVEPLRVDLMEKWYPAGIPCDVKSDTQLSQDQNS
                     QDQTNQDKDSIEEINQDQNSFFENIINQKGIQIITRLGDRYTEVQREKYLKLIASMKD
                     SIEREYKIEIRGQDYFDITNKMLTRLFNSINEKLNLGEEIDEMKFINTTARNHWEDIA
                     KQEKASRLDMLETLKKIG"
     gene            complement(4239..4566)
                     /locus_tag="IWM59_001007"
                     /pseudo
     CDS             complement(4239..4566)
                     /locus_tag="IWM59_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4575..4772)
                     /locus_tag="IWM59_001008"
     CDS             complement(4575..4772)
                     /locus_tag="IWM59_001008"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9979055.1"
                     /translation="MFQINEDFSIRIRERRGKLMITKTKAAELIGISRQTLKKIECFE
                     VQSVSKNVYQKIIGWLLKSDY"
     gene            4939..5313
                     /locus_tag="IWM59_001009"
     CDS             4939..5313
                     /locus_tag="IWM59_001009"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9979056.1"
                     /translation="MTQKNRIKETREEQNLTLKELSNLSDIPYQTLRNYEIGKRKPRN
                     DEVWWKLGTILNVRGPYLKGEDSFKTNDELHIYYLKKSHTNFELSKKEEIIDIIDKLN
                     EDGLDEALRQLALLSKIDDFKR"
     gene            5403..6569
                     /locus_tag="IWM59_001010"
     CDS             5403..6569
                     /locus_tag="IWM59_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9979057.1"
                     /translation="MSIQKYKTKSGTRYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDMGYHRPSNDLFRTVYEEWLPSYKNRVQETTYQRTKDLFRLHILPKFG
                     DKSISKITPIYCQNAINEWAVKYTNIKQLKSYSSQIFEYAIFAELLQRNPMTNTKLPK
                     QEKKESENYFSLEELKEFLEILNKEESLKHILIFQLLITTGVRKGELSALRWADINFE
                     EQLLHIGKSYATIRTEDPNATRKTIRIQKNTKNVSSERILPIDTQTIEMLKLWKKEQA
                     EVLLQFGINANTKKQLIFTYVNANGDVNQPLHADYSNNIMRRLEKKYKFKHVTIHGLR
                     HTHATLLLEGGASIKEAQDRLGHKNAETTLNTYSHVTEKAQRNAVDKFIAYTGL"
     gene            complement(6668..7060)
                     /gene="rpsI"
                     /locus_tag="IWM59_001011"
     CDS             complement(6668..7060)
                     /gene="rpsI"
                     /locus_tag="IWM59_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9979058.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(7076..7519)
                     /gene="rplM"
                     /locus_tag="IWM59_001012"
     CDS             complement(7076..7519)
                     /gene="rplM"
                     /locus_tag="IWM59_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9979059.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(8038..8505)
                     /locus_tag="IWM59_001013"
     CDS             complement(8038..8505)
                     /locus_tag="IWM59_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9979060.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8625..10163)
                     /locus_tag="IWM59_001014"
     CDS             complement(8625..10163)
                     /locus_tag="IWM59_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9979061.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            10243..10929
                     /locus_tag="IWM59_001015"
     CDS             10243..10929
                     /locus_tag="IWM59_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9979062.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(11130..11657)
                     /gene="msrA"
                     /locus_tag="IWM59_001016"
     CDS             complement(11130..11657)
                     /gene="msrA"
                     /locus_tag="IWM59_001016"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9979063.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11741..12418)
                     /gene="pgmB"
                     /locus_tag="IWM59_001017"
     CDS             complement(11741..12418)
                     /gene="pgmB"
                     /locus_tag="IWM59_001017"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9979064.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(12415..13545)
                     /locus_tag="IWM59_001018"
     CDS             complement(12415..13545)
                     /locus_tag="IWM59_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9979065.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(13545..15728)
                     /locus_tag="IWM59_001019"
     CDS             complement(13545..15728)
                     /locus_tag="IWM59_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9979066.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15725..16750)
                     /locus_tag="IWM59_001020"
     CDS             complement(15725..16750)
                     /locus_tag="IWM59_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9979067.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16795..17622)
                     /locus_tag="IWM59_001021"
     CDS             complement(16795..17622)
                     /locus_tag="IWM59_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9979068.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17619..18686)
                     /locus_tag="IWM59_001022"
     CDS             complement(17619..18686)
                     /locus_tag="IWM59_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9979069.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18712..19548)
                     /locus_tag="IWM59_001023"
     CDS             complement(18712..19548)
                     /locus_tag="IWM59_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9979070.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(19545..20414)
                     /locus_tag="IWM59_001024"
     CDS             complement(19545..20414)
                     /locus_tag="IWM59_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9979071.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(20484..21767)
                     /locus_tag="IWM59_001025"
     CDS             complement(20484..21767)
                     /locus_tag="IWM59_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9979072.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21781..23466)
                     /locus_tag="IWM59_001026"
     CDS             complement(21781..23466)
                     /locus_tag="IWM59_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9979073.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23741..24766
                     /locus_tag="IWM59_001027"
     CDS             23741..24766
                     /locus_tag="IWM59_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9979074.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(25361..29014)
                     /gene="rpoC"
                     /locus_tag="IWM59_001028"
     CDS             complement(25361..29014)
                     /gene="rpoC"
                     /locus_tag="IWM59_001028"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9979075.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(29093..32710)
                     /gene="rpoB"
                     /locus_tag="IWM59_001029"
     CDS             complement(29093..32710)
                     /gene="rpoB"
                     /locus_tag="IWM59_001029"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9979076.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            33438..34406
                     /locus_tag="IWM59_001030"
     CDS             33438..34406
                     /locus_tag="IWM59_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9979077.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            34424..35329
                     /locus_tag="IWM59_001031"
     CDS             34424..35329
                     /locus_tag="IWM59_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979078.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            35326..36132
                     /locus_tag="IWM59_001032"
     CDS             35326..36132
                     /locus_tag="IWM59_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9979079.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            36289..37257
                     /locus_tag="IWM59_001033"
     CDS             36289..37257
                     /locus_tag="IWM59_001033"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9979080.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(37574..38098)
                     /locus_tag="IWM59_001034"
     CDS             complement(37574..38098)
                     /locus_tag="IWM59_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9979081.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            38385..>39581
                     /gene="scm"
                     /locus_tag="IWM59_001035"
     CDS             38385..>39581
                     /gene="scm"
                     /locus_tag="IWM59_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9979082.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDST"
BASE COUNT        11371 a         8369 c         7136 g        12705 t
ORIGIN      
        1 aagttttctc gaacatctac tgagcgccta tcttgactac ttgtagaatt ttgtggattt
       61 tctttgactg atttttctaa tccggtattt gaatttttag cttcttggat tctttcttta
      121 gtcaacgctt ctttttcgtc catattagcg cctgtacgcc cttctacacg tttccttaac
      181 atttcctcac gattcattgc ttcaggcgtg tattttgctt cctgtcgctt ctgattaatt
      241 aattcttcag cagacttagc ttgaccgtgc atagcatttt tacgttcttc aatatcttgt
      301 ttaacttttt gctgaattgc acttgcagga ttaatatttg attcagcatt atttttcatt
      361 gcttcacgca tacgttcaga actagacata attctatcag cgttttcaac tttagcgcca
      421 tcttttctat ccgaaagacg tgttggcata gaatcagcat attttccagt ttcatcttct
      481 ttatttttag ctactatagg tttgctattt ttattaatag cggacatggt accatcatct
      541 aaaccttctt tgaatttatt cttggtatca ttagccatat cacgcatact cttaaatggt
      601 tttgttgctc tttcactagc cattgtagct ccatccgaag ctaagcctga taatctacgt
      661 tcacgagcat acccaagcgt tttccccgtt ttattagctt tttgagaaag tgattgacca
      721 aagttgcgtt caggttttcg ttcttctcct tccgcttgat cgtttccacc actatttttc
      781 ccacctttag atcggttcat accacctgtt cctttacgaa acagcattgc acccatgcca
      841 aaagcagaag caagttgccc atagccttct ttgagtccaa tatccacacc aaagtaacgc
      901 atgactgttc cactgccttt gattaaaaca aacacagcac agatataagc aattgttttt
      961 ataaagatat ttgttgagat accttcacca gcatttagat aattgacaaa cattgcaaac
     1021 atagacaatc cgaacccttg aaatccgact gtcaaataac actgtaaaat atctgacaaa
     1081 acttgttttg atcgttgacc tgtctctaag tcagtagcaa ataccaaaac tccaagtaca
     1141 cgtttaaatg ctaactcgag tattgctgta attataatga aagcactaaa aacatacgct
     1201 actgctaaag caatcaatcc aaatgaaatt ccccacaaat tcgcttgata gcgataatat
     1261 cctgatttga taccatcaac aaaggttgac aaaaaattat ctgaaaattt agtagcaaca
     1321 aattcattat cagaattttc aactaattca taacgcaggt tatcagctgt tgggttctct
     1381 ttttccattt tatcaattac attatctgtc aatacttgcg taagatccgt tttccaaaaa
     1441 ttttcttttg ttagattatt ccggtcattc gttttgttaa tcattgaata ctgatctgtt
     1501 ttattgatat aagctaaatc agtgacccct tgttcaatca gcgaccacgc cacgccttcg
     1561 tccgaatttg taatcgtagt cgtatcatta taaaatgttt tagaaaactc tattccactg
     1621 tgaatcaaag tcggcattaa aagaatcaat gctactgaca ttacaacatt cattccaact
     1681 gattttagat caaccgttcc tttacctgta atcattttat aaccaataat cactagtgaa
     1741 agtatcatta agccaacaac aatggtactt aaaactgatt gatagacttt attcaatccg
     1801 gtactttcta caaagctaaa caaatctaaa acctcgggaa tcatattttg aataccatcg
     1861 acaatagcga acattgattt tataacagac cagcctatcc acataccgaa ctggctgtaa
     1921 aaatttccgt tatatttttt aagataattc tgataaagtg gattagttaa aaactcagtt
     1981 tgactctcag tatcatcaaa ccagttgaaa atactgcctt ctgcaacgtt actcgtaaaa
     2041 ataatcatag ctatgagaat aagaaaacta atcaacaacc aatttatttt tctattatca
     2101 atcttttttt taattatttt cattgttacc tccctcagcc cattttttta ggctctcaga
     2161 aagcttctga gccatttttt tattaacgat gatgtgagtt acgtaatcat cattaacagc
     2221 tcttaaatct agaacaatag tatttgagca gatactctcg attaactcaa gagtatctgc
     2281 gttatgatca agacagtaaa tcttatctac tagatgattt tcaatcaaaa taacccctcc
     2341 cgtagtcaaa gttctcaatt ttatgaagat atccctctac ttcatttaaa tcaattctat
     2401 tcttattgct gactaacccg ttgtaaaatt ctttttgtag attatattta tctgtttctt
     2461 gaatcttttt atataaatca accagggagt ccctgtcttt aaaaacttgc ttgaattgtt
     2521 cgcttgtata tcggttttca ttaattgcaa caactgcttg aatttcaggc catttttctt
     2581 tcaacttaaa ataacgggga acgctgaaat agttctcaaa aaagaagtct cgtgcttcat
     2641 agtattctct attttgtctt tttatatcct cgacaatttc gtatcgtgta gagatcttca
     2701 aagctgaact ttcgttaatt tctgttcctt ttcgaaagac tttcactttt gaagggcgtt
     2761 caattttata atttatcccc tttttacttg aataactttt gatacgacca tcaaattgaa
     2821 ccatatctcc tttttttaac cacccaaatt ttagaaattg tttagttaaa ttcatccaaa
     2881 tgtggtctgt gaccaactct aagttatccc cttgaacaat ttttaaattc gataacataa
     2941 aagtagcatt ttcatactct tgaccaaact tgtatttatc ctttttatat ccgttaaatt
     3001 caaaggtacc ttgaaatgta tgccgtttct tatctcctaa ttttttcaat ttttctctca
     3061 aaataatcaa ctccatcatt taatatattt ttgtgatggt agatactctc gattaacaca
     3121 agagtatctg cgttgtaatc aagacaataa atttgatata ttaaccgttt ttcaatcata
     3181 ttattaatcc tttctaacct atttttttca gtgtctctag catatccagt ctactagctt
     3241 tttcttgctt agctatgtcc tcccagtggt tcctagctgt ggtgttgata aacttcattt
     3301 catcaatttc ttcaccgaga ttcagttttt catttataga attaaaaagt cttgttagca
     3361 ttttgtttgt tatatcaaaa tagtcttgac ctcgaatttc tattttatat tctctttcaa
     3421 ttgaatcttt catactagca atcaacttaa gatacttttc tctttgaact tcagtatatc
     3481 tatcacctag tctggtaatt atttgaattc ctttctgatt tataatattt tcaaaaaagg
     3541 aattctgatc ttgattaatc tcttctatac tatccttatc ttgattagtt tgatcttgac
     3601 tattctgatc ttgacttaac tgtgtatccg atttgacgtc gcatggtata ccagctggat
     3661 accatttttc catcagatca actcttaatg gttctacttg accagactgc aaaatcatat
     3721 caaattttat tggctttagc tttgagaaaa attctggttt taagttcaat aacttgaatc
     3781 gataaataga aggtttatac ctatcgattc gtatattttg attttgtaac cagtggggta
     3841 gaaacgctaa caaatcttca tttaaaaaaa atacaaaacc ttttgtaaaa agtatcttta
     3901 catcatcatc gggtgttccg agcattttcg tgtatggata agcttcgaca attccgtcat
     3961 catctgcgtt tgtgttcaga tatacgtata ataattttgc tgaatctgac atcttcataa
     4021 aacttgcatt atttaaaaat gatttagcca gcattcgttt ttctgccata aaaatcacac
     4081 ttcttcctat ttgaatgtta aaatgtagtc aaataaagtt tagctagaac tttattattg
     4141 agcgttccta gtggtctgca aactaacggg aacactcttt tttattctta ttaaaaccag
     4201 caatactagt tcaccttctt tcatcctttt acatccaatt aaatttgatg ctgtagcatc
     4261 cattttttta cctcttcttt tgaatatagt tcaattgtgt caaccacgat aataggtaat
     4321 cctaaccttt taaacttcag aaaagttcca tatgatacgt ttagaaattt tgccatttct
     4381 tttcccttaa gaaaaggtcg ttctaatccg ctgtctattc ttgcttgatc aatagcttct
     4441 tttgtcactt caaacacatg tgtcttcaac tgatttgttt gatcagtatt caacattaca
     4501 ctgactccga tttgctccat taaaaattcc cactttcata ttttcatcaa taagccaact
     4561 cgccaagttg tttgttaata atctgatttc aataaccaac ctattatttt ttgataaaca
     4621 tttttcgaca cactttgaac ttcgaagcat tcaatttttt taagagtttg ccttgaaatt
     4681 ccaattaatt ctgctgcttt agttttcgta atcatcaact ttcctcgtct ctctcgaatt
     4741 cttatactaa aatcctcatt aatttgaaac ataaacatcc ctccattttt atttttgcat
     4801 ttatccgttt atccgatgtc ggataaatag attttagtat atattatttt gatagtcaat
     4861 taaaaatata cattttggat aaaattgttc atttttttat ccaaatcata tattatagat
     4921 agtaagaggt gaacttcatt gactcaaaaa aacagaatta aagaaactcg cgaagaacag
     4981 aatttaacac ttaaagaact tagcaatctg tcggatattc cgtatcaaac tttgagaaac
     5041 tatgagattg gtaaaagaaa acccagaaat gatgaagttt ggtggaagtt aggtacgatt
     5101 ttaaacgtcc gtgggcccta cttaaaagga gaagatagtt ttaaaacaaa tgatgaattg
     5161 catatttatt atctaaaaaa aagtcataca aattttgaac tgtctaagaa agaagaaatt
     5221 atagatataa tagataaact aaacgaagac ggtttagatg aagccttacg gcagttagca
     5281 ctattatcaa aaatagatga tttcaagcgg taacttactt atcatcatgt agactatcag
     5341 gtatcataaa ctaattcctc aatcagccaa ctcgccaagt tatcagattg gagagattag
     5401 ttatgtcaat acaaaagtac aaaacaaaaa gcggtacacg atatagtgtc cgcttatatg
     5461 ttcgtaaaga tgaaaatggg aagcaagaat actatgtaaa aaaaggcttc aaaactgaaa
     5521 aagaagccaa gttacacgaa gcaagaaaaa aggtcgagat tgaagatatg ggctatcaca
     5581 gaccttctaa tgacttattt agaacagtct atgaagaatg gttgccgtct tataaaaatc
     5641 gtgtacaaga aacgacttat caacgaacaa aagacctttt tcgcttgcat atcctgccaa
     5701 agttcggaga caaatcgatt tctaagatta ctcctattta ctgtcagaat gcaatcaatg
     5761 aatgggctgt aaaatataca aatatcaagc aacttaaatc ctacagctct caaatatttg
     5821 agtatgcaat cttcgcagaa ttgttacagc gtaaccctat gacaaatacc aaactgccaa
     5881 aacaagaaaa gaaagaatcc gaaaattatt tctcactaga agaattaaaa gagtttttgg
     5941 aaattctcaa taaagaagag tctttaaaac atatcctgat ctttcaactt ttaattacta
     6001 ccggtgttcg caaaggtgaa ttatcagccc tacgctgggc agatattaat tttgaagaac
     6061 agttactaca catcggaaaa agttatgcaa caattcgcac tgaagatccg aatgctacta
     6121 gaaaaacaat caggattcag aagaatacaa aaaatgtctc ttccgaacga attttaccga
     6181 ttgatactca aaccatcgaa atgctgaaac tttggaaaaa agagcaagca gaggtacttt
     6241 tacaattcgg cattaatgca aataccaaaa agcaacttat ctttacctat gtaaatgcaa
     6301 atggcgatgt taaccaacct ttacacgcag attattccaa taatattatg agacgtttag
     6361 agaagaaata taaatttaag cacgttacta ttcatggatt gcgacacact cacgccactt
     6421 tacttttaga aggcggtgcg agcattaaag aagcacaaga tcgattgggt cataaaaatg
     6481 ctgaaactac gctcaatact tattcccatg taactgaaaa agcgcaacga aatgcggtag
     6541 acaagtttat tgcttacact ggattataga caaaaaagtt gccgtcatta cctttttcat
     6601 taccacaacg aaaaaaagca ccttccaact gaatggaaag tgcttgaaat cgttggtata
     6661 acaacgatta acgcttagag aattgtgaag ctttacgagc tttcttaaga cctggttttt
     6721 tacgttcgac catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga
     6781 agtcaggatc aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc
     6841 cagcgtatcc tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa
     6901 cagcgaaagg ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat
     6961 cttttttatt tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt
     7021 ttttacgacg gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga
     7081 ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac
     7141 cgtatacgtt caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt
     7201 ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg
     7261 atttcaatcc tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac
     7321 ctgttaattt cactttgtct gcattgatga cgatgacgaa atcgccagta tccaaatgag
     7381 gtgtgaaagt tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc
     7441 ccaatggaac atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg
     7501 ccatatatgt tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac
     7561 ttcgagtttc cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat
     7621 ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt
     7681 tctttctttt atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga
     7741 caaaagtctt gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg
     7801 tcacaagtca caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac
     7861 tcggagctga acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc
     7921 ggtattagct caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata
     7981 ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta
     8041 cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt
     8101 ggctgttttg aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac
     8161 ttcgattcct ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa
     8221 acgttctgga atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc
     8281 aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc
     8341 atctaactga gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa
     8401 acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc
     8461 tgcaactaat tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta
     8521 acattttctt acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt
     8581 acctgagaac gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca
     8641 gctaattctt ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc
     8701 gaaacaaata atggatagat tttctgatat tttttatgat cagtttcttg tactccaact
     8761 tgctctttca attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt
     8821 ctccagccta aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc
     8881 gggtaaccca gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct
     8941 gtagcacgga tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg
     9001 ttgagacaga tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt
     9061 aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa
     9121 tagggatgaa aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt
     9181 gcatcatatg gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta
     9241 tgccctgccc aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt
     9301 ttctgatcca gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta
     9361 ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca
     9421 cctaacccta gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt
     9481 ggcaaaccta gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga
     9541 aagacttgcg gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat
     9601 tggaattcat ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt
     9661 tcgccaaaca atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg
     9721 aataattcag gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga
     9781 atcggcgtcc cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct
     9841 gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct
     9901 tcatccaaca atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg
     9961 tctgaaggca actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat
    10021 tcctgatctt gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg
    10081 gatgctgctt cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata
    10141 tctaatccga ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta
    10201 gtttaagctt gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc
    10261 attggttgct gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac
    10321 tctattattt atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa
    10381 ttgattccga ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg
    10441 ttgcccttct actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt
    10501 actttttctt ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt
    10561 tcgttgatgg atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca
    10621 attttatgga ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg
    10681 tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact
    10741 ctgttgatgc tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt
    10801 cttgctagtc tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag
    10861 ttggaaaagc tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt
    10921 aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc
    10981 ttaccaaatc cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt
    11041 gttcaatcag ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac
    11101 ggtgttccat aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg
    11161 aaacctgcac gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa
    11221 taatcctgat ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg
    11281 attggcttat cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt
    11341 cgttcttctt catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac
    11401 tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa
    11461 gaaataactt ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt
    11521 gttacttgtt cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa
    11581 atgatccctg gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa
    11641 actgcttttt ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag
    11701 gaggataacg ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt
    11761 ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt
    11821 cagcagaagg aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag
    11881 ctgcttgaca accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca
    11941 attgctgtgc tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc
    12001 cgtcaataag gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat
    12061 ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag
    12121 ctattgcacc aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat
    12181 tgtttttttg ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc
    12241 caaatgacag caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt
    12301 ctttctcagt gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat
    12361 aacgagctgt gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg
    12421 attacctcct tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg
    12481 aagattatca gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg
    12541 aaaaacggcc gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca
    12601 aatagctatc acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc
    12661 ttttagactt tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac
    12721 ccgtttatcg aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga
    12781 agcaaatgct aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat
    12841 tacttgaggg gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc
    12901 attttctttc aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa
    12961 aagattagta acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt
    13021 cgttttatca actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt
    13081 atcttgatcg taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt
    13141 actacttccg ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat
    13201 cagttctcct actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc
    13261 tttcaatccg acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg
    13321 gatttctttg tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa
    13381 actgtctgct aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata
    13441 ttcagcctga acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc
    13501 tgtcagactt tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt
    13561 tccaagatca gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt
    13621 tctttcgtct ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga
    13681 tttttccaaa taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata
    13741 gaaagagttc cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt
    13801 gctcctaatg aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca
    13861 atcaggcacg cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc
    13921 tctatggcgt gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa
    13981 tgcaaattct tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg
    14041 acaagatctg cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt
    14101 aaaatagact ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg
    14161 agatacgtgt catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta
    14221 tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct
    14281 aaggccacat tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg
    14341 tattcatctg gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg
    14401 ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat
    14461 tgatcctctg ttgcttggac atactcttga acggcataag caaggtctgc actgatatga
    14521 tgttctgcgg cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca
    14581 aatttgggtg tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga
    14641 tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc
    14701 aaattctttg ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta
    14761 tcccagaaaa catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca
    14821 cttggatcat tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac
    14881 tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa
    14941 taagacatcg actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata
    15001 cttttcttaa acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc
    15061 gtaaaggacg tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt
    15121 gcttggaata cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct
    15181 gttgtctggt aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc
    15241 aactgctgtg taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg
    15301 cagtcttcgg tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga
    15361 tcagcaacac gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca
    15421 tagcgtttaa gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc
    15481 gtctcaagag aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga
    15541 ttcactaact cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt
    15601 ccccttactt gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt
    15661 ccaatggtca tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct
    15721 actttcataa ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat
    15781 tctggatgac atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc
    15841 gattcacaaa atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta
    15901 aggaaaccag ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg
    15961 cctgatcgcc acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag
    16021 tattcagttg gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta
    16081 ctgtgaattt gcttggatcc catttcccga atttccctga attttttttc gtaccaattt
    16141 tttggaagct gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg
    16201 tatcaagcat gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga
    16261 taaacgttcc ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa
    16321 tatccccgag aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct
    16381 ggaatccata agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt
    16441 gatcagctgt catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag
    16501 ccaatacatg ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa
    16561 tctcattcag catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa
    16621 agtcttctgc tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc
    16681 ttttttgata ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta
    16741 ctttcttcat ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc
    16801 tttctctaaa tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga
    16861 ttcctgataa attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc
    16921 ttcatactca ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc
    16981 tggttggtag cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt
    17041 caatgctttt gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg
    17101 cacatgtttc aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat
    17161 atggtcttgg tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac
    17221 tttatctaaa taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg
    17281 tgaaaccatc ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc
    17341 tttgcctcca acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag
    17401 acgccgttct tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac
    17461 tgggatccct gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt
    17521 accgtcgatt tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat
    17581 gtctcttggg aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact
    17641 tgaacgccca ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct
    17701 gcgctttctg caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc
    17761 atcaataatt cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg
    17821 tttggttcac tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga
    17881 ccaagattaa acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca
    17941 tagccgatcc ctattaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact
    18001 gaatctactc cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata
    18061 ggatctagcg tgtaatcggc accgtgtttt tctgctaatt ctctacgctg cgctatggga
    18121 tcgatcccaa cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg
    18181 atagcgccta aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata
    18241 ccacctaaag cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga
    18301 tcaagtttac gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt
    18361 ccataagtgc agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg
    18421 atcgtaccga caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt
    18481 ccttcttctc ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga
    18541 cttaatcctc taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg
    18601 atacgtactt cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt
    18661 ggttctaatg caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt
    18721 tacgccgcct tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat
    18781 gactggtaaa gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt
    18841 actgaataat gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat
    18901 cgaagcaaat aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat
    18961 tccagaaata gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc
    19021 gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa
    19081 tatcgcagta ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata
    19141 caaacctaga ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat
    19201 ataaacggtg taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa
    19261 agcgtatgcc ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac
    19321 taaactattt ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata
    19381 atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga
    19441 atccttgaat gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa
    19501 caaaatcgtc gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc
    19561 ttctttcgtg agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct
    19621 gctgcttttc caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata
    19681 ttgactttat tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt
    19741 aaaacaatca cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc
    19801 tgccaagcat ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc
    19861 aaaatagcca aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg
    19921 accataaaga agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc
    19981 aacctatcta ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa
    20041 gcaaaaatca aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt
    20101 ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg
    20161 cctgctacaa ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta
    20221 tcacttaaaa tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc
    20281 agcggcatat cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca
    20341 gctaataaaa gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc
    20401 cttggacgat tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg
    20461 aacaccattg gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga
    20521 tcgacatctt cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt
    20581 gtgatatctc ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca
    20641 tcaaaagcac tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct
    20701 ttgtaaccat ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc
    20761 aagacccact tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc
    20821 tctttttgtt tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca
    20881 gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa
    20941 acagaaggca aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca
    21001 tctttgattt ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa
    21061 aacgacaatg cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa
    21121 acatttgctc catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca
    21181 gcagtaggca tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt
    21241 tttaaaatat catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg
    21301 tcataccaaa taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct
    21361 tctgaacgaa caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata
    21421 acgttttgga ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc
    21481 tgactgactt ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta
    21541 ttgaacgctg attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca
    21601 aaatcagcaa ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg
    21661 aattgaatcg ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga
    21721 agaaatccag tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc
    21781 ttattttatt attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg
    21841 actgcaatgg ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata
    21901 acctgctgat tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg
    21961 aataaaactg ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa
    22021 ggctttttct ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct
    22081 taatggatct gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat
    22141 ttttcgattc tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact
    22201 ttgaatataa attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa
    22261 gcgtttgctt ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc
    22321 gtttatctca tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc
    22381 ctctttcttc agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac
    22441 cggattcaat ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga
    22501 tgcgaaatcg atggttttta accattcttt caacttactt gtattacccg aacgaatcgc
    22561 ttgaagggtc aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt
    22621 cccaaaataa ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc
    22681 tggagctgct tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag
    22741 gtgtatacaa gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa
    22801 tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg
    22861 atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt
    22921 cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct
    22981 tgtatctgta tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa
    23041 atattcctga aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa
    23101 ccgtgtttcg cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc
    23161 ctgataattt acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg
    23221 tacaactcta aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt
    23281 tggttgctgc tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc
    23341 ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat
    23401 catagaggcg gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt
    23461 atacatcgga ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga
    23521 aaacgcttac tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata
    23581 agaaagctta tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat
    23641 gcaagctatt cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga
    23701 attattctaa aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga
    23761 cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc
    23821 aaatatctct gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga
    23881 accgagcaaa attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc
    23941 tcatcgtact tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat
    24001 ttctgccgtt tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga
    24061 agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat
    24121 tttaagctct acgatggatg aaacacttct ttttgaattg gatcaatata atattcctat
    24181 tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa
    24241 tttccaggat agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg
    24301 ctgtctccat gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca
    24361 atgcttattt gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg
    24421 cttagaagat agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc
    24481 gattttttct accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg
    24541 aaagcagata cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct
    24601 attttcgcca cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac
    24661 agaactttta ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc
    24721 aaccgagatt actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa
    24781 agaacagaaa cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc
    24841 gttaccccct cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg
    24901 tggataacta taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt
    24961 aaccacgttg tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca
    25021 atccatgtta tttggggata cttatctcta aaacataaat agaataacag acatgtctac
    25081 tggttttgtt ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg
    25141 ctctatcttc ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga
    25201 ttgacttctc ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt
    25261 aagatccttt cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag
    25321 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
    25381 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
    25441 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
    25501 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
    25561 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
    25621 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
    25681 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
    25741 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
    25801 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
    25861 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
    25921 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
    25981 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
    26041 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
    26101 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
    26161 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
    26221 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
    26281 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
    26341 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
    26401 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
    26461 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
    26521 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
    26581 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
    26641 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
    26701 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
    26761 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
    26821 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
    26881 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
    26941 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
    27001 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
    27061 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
    27121 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
    27181 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
    27241 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
    27301 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
    27361 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
    27421 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
    27481 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag
    27541 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
    27601 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
    27661 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
    27721 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
    27781 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
    27841 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
    27901 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
    27961 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
    28021 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
    28081 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
    28141 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
    28201 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
    28261 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
    28321 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
    28381 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
    28441 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
    28501 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
    28561 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
    28621 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
    28681 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
    28741 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
    28801 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
    28861 aaatccgctc acagaacaac ccttcacgtt caggttttaa ggtacgatag ttaatcgtct
    28921 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
    28981 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
    29041 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
    29101 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
    29161 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
    29221 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
    29281 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
    29341 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
    29401 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
    29461 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
    29521 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
    29581 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
    29641 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
    29701 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
    29761 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
    29821 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
    29881 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
    29941 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
    30001 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
    30061 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
    30121 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
    30181 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
    30241 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
    30301 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
    30361 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
    30421 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
    30481 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
    30541 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
    30601 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
    30661 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
    30721 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
    30781 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
    30841 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
    30901 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
    30961 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
    31021 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
    31081 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
    31141 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
    31201 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
    31261 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
    31321 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
    31381 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
    31441 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
    31501 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
    31561 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
    31621 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
    31681 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
    31741 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
    31801 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
    31861 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
    31921 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
    31981 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
    32041 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
    32101 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
    32161 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
    32221 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
    32281 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
    32341 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
    32401 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
    32461 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
    32521 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
    32581 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
    32641 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
    32701 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
    32761 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
    32821 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
    32881 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
    32941 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
    33001 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
    33061 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
    33121 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
    33181 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
    33241 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
    33301 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
    33361 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
    33421 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
    33481 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
    33541 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
    33601 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
    33661 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
    33721 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
    33781 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
    33841 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
    33901 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
    33961 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
    34021 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
    34081 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
    34141 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
    34201 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa
    34261 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
    34321 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
    34381 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
    34441 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
    34501 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
    34561 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
    34621 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
    34681 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
    34741 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
    34801 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
    34861 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
    34921 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
    34981 cggttatatg atttctaatg gatgcattgc tctttctggc tccttacttg cgcaaaacaa
    35041 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
    35101 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
    35161 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
    35221 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
    35281 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    35341 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    35401 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    35461 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    35521 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    35581 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    35641 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    35701 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    35761 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    35821 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    35881 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    35941 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    36001 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    36061 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    36121 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    36181 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    36241 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    36301 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    36361 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    36421 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    36481 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    36541 ttgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt
    36601 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    36661 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    36721 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    36781 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    36841 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    36901 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    36961 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    37021 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    37081 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    37141 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    37201 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    37261 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    37321 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    37381 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    37441 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    37501 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    37561 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    37621 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    37681 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    37741 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    37801 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    37861 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    37921 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    37981 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    38041 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    38101 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    38161 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    38221 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    38281 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    38341 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    38401 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    38461 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    38521 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    38581 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    38641 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    38701 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    38761 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    38821 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    38881 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    38941 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    39001 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    39061 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    39121 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    39181 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    39241 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    39301 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    39361 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    39421 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    39481 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    39541 caagtgatag cacggacaca tcagcttcaa gtgacagtac a
//
LOCUS       DACDYM010000020        37818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDYM010000020 DACDYM010000000
VERSION     DACDYM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.020"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(159..1469)
                     /locus_tag="IWM59_001036"
     CDS             complement(159..1469)
                     /locus_tag="IWM59_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9979083.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IWM59_001037"
     CDS             complement(1481..2899)
                     /locus_tag="IWM59_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9979084.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IWM59_001038"
     CDS             complement(3139..4707)
                     /locus_tag="IWM59_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9979085.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IWM59_001039"
     CDS             complement(4711..5121)
                     /locus_tag="IWM59_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9979086.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IWM59_001040"
     CDS             complement(5118..6041)
                     /locus_tag="IWM59_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9979087.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IWM59_001041"
     CDS             complement(6038..6916)
                     /locus_tag="IWM59_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9979088.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IWM59_001042"
     CDS             complement(6903..8240)
                     /locus_tag="IWM59_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9979089.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IWM59_001043"
     CDS             complement(8243..9346)
                     /locus_tag="IWM59_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9979090.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IWM59_001044"
     CDS             complement(9420..10928)
                     /locus_tag="IWM59_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9979091.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IWM59_001045"
     CDS             complement(10894..11586)
                     /locus_tag="IWM59_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9979092.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IWM59_001046"
     CDS             complement(11608..12582)
                     /locus_tag="IWM59_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9979093.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IWM59_001047"
     CDS             12882..13754
                     /locus_tag="IWM59_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9979094.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="IWM59_001048"
     CDS             complement(14212..16410)
                     /locus_tag="IWM59_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9979095.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="IWM59_001049"
     CDS             16642..17634
                     /locus_tag="IWM59_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9979096.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="IWM59_001050"
     CDS             17694..19115
                     /locus_tag="IWM59_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9979097.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="IWM59_001051"
     CDS             19108..20607
                     /locus_tag="IWM59_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9979098.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="IWM59_001052"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="IWM59_001052"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9979099.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="IWM59_001053"
     CDS             22199..22882
                     /locus_tag="IWM59_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9979100.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="IWM59_001054"
     CDS             22884..23720
                     /locus_tag="IWM59_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9979101.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="IWM59_001055"
     CDS             23731..25485
                     /locus_tag="IWM59_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979102.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="IWM59_001056"
     CDS             complement(25761..26102)
                     /locus_tag="IWM59_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9979103.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="IWM59_001057"
     CDS             complement(26170..28341)
                     /locus_tag="IWM59_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9979104.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="IWM59_001058"
     CDS             28552..29409
                     /locus_tag="IWM59_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9979105.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="IWM59_001059"
     CDS             complement(29480..30337)
                     /locus_tag="IWM59_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9979106.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="IWM59_001060"
     CDS             complement(30322..33024)
                     /locus_tag="IWM59_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9979107.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="IWM59_001061"
     CDS             complement(33037..34326)
                     /locus_tag="IWM59_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9979108.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="IWM59_001062"
     CDS             34476..35522
                     /locus_tag="IWM59_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9979109.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="IWM59_001063"
     CDS             35524..37677
                     /locus_tag="IWM59_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9979110.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11174 a         7789 c         6725 g        12130 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatca
//
LOCUS       DACDYM010000021        37702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDYM010000021 DACDYM010000000
VERSION     DACDYM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.021"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(112..1056)
                     /locus_tag="IWM59_001064"
     CDS             complement(112..1056)
                     /locus_tag="IWM59_001064"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9979111.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1352..2296)
                     /locus_tag="IWM59_001065"
     CDS             complement(1352..2296)
                     /locus_tag="IWM59_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9979112.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2517..3731
                     /locus_tag="IWM59_001066"
     CDS             2517..3731
                     /locus_tag="IWM59_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9979113.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3753..5768
                     /locus_tag="IWM59_001067"
     CDS             3753..5768
                     /locus_tag="IWM59_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HAP9979114.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5851..7224)
                     /locus_tag="IWM59_001068"
     CDS             complement(5851..7224)
                     /locus_tag="IWM59_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9979115.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7250..7534)
                     /locus_tag="IWM59_001069"
     CDS             complement(7250..7534)
                     /locus_tag="IWM59_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9979116.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7536..9551)
                     /locus_tag="IWM59_001070"
     CDS             complement(7536..9551)
                     /locus_tag="IWM59_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9979117.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9730..10035)
                     /locus_tag="IWM59_001071"
     CDS             complement(9730..10035)
                     /locus_tag="IWM59_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9979118.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10124..11155)
                     /locus_tag="IWM59_001072"
     CDS             complement(10124..11155)
                     /locus_tag="IWM59_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9979119.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11362..12237
                     /locus_tag="IWM59_001073"
     CDS             11362..12237
                     /locus_tag="IWM59_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9979120.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12389..13231)
                     /locus_tag="IWM59_001074"
     CDS             complement(12389..13231)
                     /locus_tag="IWM59_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9979121.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13323..15200)
                     /locus_tag="IWM59_001075"
     CDS             complement(13323..15200)
                     /locus_tag="IWM59_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9979122.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15197..16618)
                     /locus_tag="IWM59_001076"
     CDS             complement(15197..16618)
                     /locus_tag="IWM59_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9979123.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16621..20010)
                     /locus_tag="IWM59_001077"
     CDS             complement(16621..20010)
                     /locus_tag="IWM59_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9979124.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20267..21658
                     /locus_tag="IWM59_001078"
     CDS             20267..21658
                     /locus_tag="IWM59_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9979125.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21731..22378)
                     /locus_tag="IWM59_001079"
     CDS             complement(21731..22378)
                     /locus_tag="IWM59_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9979126.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22386..23072)
                     /locus_tag="IWM59_001080"
     CDS             complement(22386..23072)
                     /locus_tag="IWM59_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979127.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23078..24151)
                     /locus_tag="IWM59_001081"
     CDS             complement(23078..24151)
                     /locus_tag="IWM59_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9979128.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24373..25047
                     /locus_tag="IWM59_001082"
     CDS             24373..25047
                     /locus_tag="IWM59_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9979129.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25052..26392
                     /locus_tag="IWM59_001083"
     CDS             25052..26392
                     /locus_tag="IWM59_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9979130.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26541..27527)
                     /locus_tag="IWM59_001084"
     CDS             complement(26541..27527)
                     /locus_tag="IWM59_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9979131.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28251..29282
                     /locus_tag="IWM59_001085"
     CDS             28251..29282
                     /locus_tag="IWM59_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9979132.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29391..30923
                     /locus_tag="IWM59_001086"
     CDS             29391..30923
                     /locus_tag="IWM59_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9979133.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31062..31874
                     /locus_tag="IWM59_001087"
     CDS             31062..31874
                     /locus_tag="IWM59_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9979134.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            31864..32349
                     /locus_tag="IWM59_001088"
     CDS             31864..32349
                     /locus_tag="IWM59_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9979135.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32372..32917
                     /locus_tag="IWM59_001089"
     CDS             32372..32917
                     /locus_tag="IWM59_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9979136.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(32990..34633)
                     /locus_tag="IWM59_001090"
     CDS             complement(32990..34633)
                     /locus_tag="IWM59_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9979137.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(34762..36990)
                     /locus_tag="IWM59_001091"
     CDS             complement(34762..36990)
                     /locus_tag="IWM59_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9979138.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37023..37502)
                     /locus_tag="IWM59_001092"
     CDS             complement(37023..37502)
                     /locus_tag="IWM59_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979139.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        10984 a         7151 c         6869 g        12698 t
ORIGIN      
        1 ttttaaccat tctgtttatt tgcttaccta ttagatatca ataaaaaact gcttaacagc
       61 tatttcagct gttaagcagt tttaggattt ttagttcata ttcattgttt cttataatac
      121 agtgttcaat acttctctta aagcacttgt tgatttgctg aacattgctt tttcttcttc
      181 gttgattgat aattcaacaa cttcacgaac accattttcg tcaacgattg atggcacacc
      241 tgtaaagata tctttttcgt cgtattcacc agttaaataa gcagatacag gcaatacagc
      301 ttgttcgtta ttcaagatag cttttacgat acgtgcagta ctcataccaa taccatagta
      361 agttgctttt ttacgatcaa tgatctcata agcagcatta cggactttat cagcaatcac
      421 atctaattca tcttgggcaa tacgatggtc tttttctaca atttcaaaca ctggtttacc
      481 accgacagtt gtatgtgacc atgcagcaac ttctgaatca ccatgttcac ctaagatata
      541 gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg atttctttac ggaaacgagt
      601 tgtatccaat gttgttccag taccgataac acgtgatgtt ggcagaccag attcgttcca
      661 agcgatatat gtcaaaatat ctactgggtt tgaagcaaca acgataatac cgtcgaatcc
      721 tgatcccatg atttctttta cgatttggcg catgattgac gcatttgttt taactaaatc
      781 taaacgagat tgaccaggtt tttggttgat accagctgtg ataacaacga tatcagcatc
      841 tttacattct tcatagccac ctgaccatac agccacattt tcatcgcccc aagccatacc
      901 atctaaaagg tccaatgctt ccccttctgc tttttcttga ttcacatcga tcaatactaa
      961 ttcattagag attccttggt tgatcattgc ataagcgata cttgttccaa caaaacctgt
     1021 accgacgatt actactttac gacttgtttt tttcatgaat aaacagctcc ttttcataac
     1081 tatttgttac tttcatgaaa agtatagtct tgtttattca ttttatcaac atgttaatta
     1141 taatagttgt tattataatt tttgatggaa acgaattcct gaatacgcat aacagataaa
     1201 tgacaaatgt ttacacctat atcccctgat aatacaacca aaagtttaga actgaaaata
     1261 aatccaaaaa aattcgctgc aacgatataa taagacagca atcgcaccta aaacaatttt
     1321 ccttcatatg ctttagtgaa agattttctt cttaatgttt atttctccat tcattgggag
     1381 tacatgttga atacttcttg aataatttga ataaagaagt cgtttgttta tatccgatac
     1441 gattagcgat ttcttccaac gaaaaactcg tttcttgaat cagttccaac gcaaagtcga
     1501 attttttctt tagaaccagt tcttgaaagg aagcatcaaa atacattttc aatttattac
     1561 taagatagac cggattgtag ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa
     1621 aatgcttatc gatataatct aatactctaa gcatctcatt ttctgcgcat ccatctgctg
     1681 atgaaaagct ttgttgttct aataaatgag tcaattcgat cagtaatagt gatgtataga
     1741 gatttaacga acggatctta tgactgtatg tatcaaaata aatcaacagc atattttcaa
     1801 tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc
     1861 ctttttcttt tgatgcattt atcaaaaaag aagaaagtag attatttgtg cttgccaaat
     1921 gattcaatat ctctgtagat aaggtttctt cttttaataa gatgttgatt aaaatatcct
     1981 cttgccctgt gtattcgatt ctttgagtag cttctttatc aatgatcacc acgtcacttt
     2041 cttccatctc tatcctttct ccattaataa tttgtaaaca tgtaccgcta tacatataat
     2101 tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc atatcgacgg tgtttactga
     2161 tatgaatatt tccctgttga aatagatccc ctcggtggat ttttggcggt gtctgaagat
     2221 ggtaagctaa gtttaaatca ttgatattca ttccttgttc acgctgcaga gtttcgattg
     2281 attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt ccaattgact ccctcctttt
     2341 tgtaagcggt ttctttcgct tattagttca cgctgtactg ctattatatg caaagctatt
     2401 tttggtttca taaagacttt ctatggttgg gacatgaact tcctgtgatt ccgctattga
     2461 gagaataacc acttcatact atatttttga taagcgctta caaaaatgaa aggaacatgc
     2521 ttatgaataa aaagatgatg aaattttctc tgctgtcagt ctctcttatt ttgacttctg
     2581 ctggtgcagt cacgtctgca attcccagta tgtcagctac ttatgccacg cgttctttat
     2641 ctgctgttga attattaacg actgctccct cttttatggt cactattttt gttttgatta
     2701 gtagttttat tgctaggaaa atcggacaaa aacaaactgt gatcatcgga ttagtgattg
     2761 catcgattta tggtatcctc cccctcttct cacaaagtta cagtattgtc ttagcttcaa
     2821 gagctggact aggtattggc tttggattga tcaattcttt ggcagtcagc atgattgctg
     2881 ccttttatca cggagatgaa cgagcctcta tgattggttt tcaaagtgca tttcaaggat
     2941 ttggtgcagc tttaatgaca tttgttgcag gacagctagt acagttcggt tggcaatata
     3001 cctttttagt ctacgccatc agcttaccta ttcttgtctt gtttgttttg tttgttccta
     3061 cacctgaaac aactgaactt caatcacatg gcacagcccc aacaagacaa gtaatcaaaa
     3121 cgaatcctaa aatgattagt tatggtattt ttctcttgct ggtcatcgtg gtatataatg
     3181 ctgtatcgat taaattagcc acagtcctga cgacttattc tctaggaaac gaaactaatg
     3241 cagcgaatat cttaacaatc atgcaattgg caagtatggc tgccggagtt tgttttggct
     3301 ggcttcagac aaaagcacat cgctatatgc ttacactttc tttactactg atgggaatcg
     3361 gctttgtatt gattgccact gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa
     3421 caggaatttc tttttcgtta ttcattcctt acttattcaa ccaagtaagt atccaagcgc
     3481 ccaagtacgc ggagagtttc aatacttcaa tcttattggt tggcgctaat ttaggtactt
     3541 tcattgcacc atatggtttg ctctttctct cttttgcttc tcttgggagt aaaacacttg
     3601 ctgttttcgt caatggcagt atcttgctgt tgattagcgc gcttattagc cttctagtag
     3661 tacacaggac gaatagaaag acagtgataa gagtagatga agaaataaaa gaacaattga
     3721 caacaaaata aatttgatgg aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat
     3781 ggctttggta tcctggagac tttgagatcc gccatggact gctacaaaat ttccaacggg
     3841 aagaacgagg ttttgactgg ccagcgtact ggtacatgga tgattgccat cggaacgtca
     3901 agtttaagag atattacttt cttgatcaac cttccatgtt taaagtgacg atccaaggag
     3961 ttgggtatgt agaaattaat ggacaaaaac atccatgtgg aaaatggctg acttgtccag
     4021 ctggtaaagc aaaaattcgt atctttgtag gacatacatc agggctacca gcgatgttta
     4081 tcgagggaga cgaagtaaaa tcagatattg gctggacagc aagtaatttt attgaagaat
     4141 atccagctgg ctggagtccg ctttatgtcg atatcgcaaa agatccaaat caaatctatt
     4201 atcaaaaaga atgcgtccat ccagtcactg aaacggagat tggcggagga atcttattcg
     4261 attttgggcg agcagtgaat ggctgtgtag aactaatatc tctggatgac catatcgaat
     4321 ctatcactct atgttatgga gagtccgatg tagaagctat ggatatcgat tattgctact
     4381 ataagcaaat aggaatgaca agaaaaatga aagctcgtaa acgcgctttt cgctatttgt
     4441 ttataccaga ggtttctact ggaatggttc aacttcaagt ttttcatgaa tttttatctc
     4501 tttcgaaaca agctgctttt tcaagcgatt cttctttact gaatcagatc tggtcagtat
     4561 cagaagaaac cttcaagtta tgtagtgatt tgttttttat cgacggtatc aaacgagatc
     4621 gctggatttg gtctggagat gcgtatcaat cattcatgat caatcaatat cttttttttg
     4681 acgaggaaat caataaacga acgatattag cattaagagg acaaaatgaa atcaagcagc
     4741 atatgaatac tatcgttgac tattcgatct tgtggttgat tggtatcgaa aaccactata
     4801 tgatgacgaa agactatgaa tttttacgca tcgtctaccc taagatgcaa tcattaatga
     4861 gatacttgat ggagcaaacg aatgaattag ggtttattta tgggagagca aaagattgga
     4921 tatttattga ttggtctgaa atggataaag aaggaacgct tgctgcagaa caagtcttat
     4981 tactgaaagc ctatcattcc atgatcactt gcggagatat cttagctgaa acatatggag
     5041 agaacgaaaa ttatcatact catctctatc aagaaaaata tcaactattg aaacaaaacc
     5101 tatttgacta cttttgggat gaagaacttg gcgcttttat cgattgctat gaatctggga
     5161 gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt attcgatttt gctaacaaag
     5221 accaacagca atcgatctta aagaatgtct tattgaatga cactatcaca cagattacta
     5281 ctccatattt caaattctat gaacaagatg ctttatgcaa actcggacag acgaatatcg
     5341 tctaccaaac gattcttgat tattggggag ggatgttatc tcatgatgcg gtaactttct
     5401 gggaagaata cgaccctact caaacaggaa atgaaaagta cagtatgtat ggagatcctt
     5461 atggaaaaag cttatgccat gcatggggag caagcccaat ctatctttta ggacgctatt
     5521 ttatcggttt gtatccaaca aagccaggtt atgaagaatt tatgatacaa cctaatctag
     5581 attcatttga aacactgaat tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa
     5641 aaacaaaaga taagatcgtg attacttctg atagaacagg cggaacagtg aaaatgaatg
     5701 gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt aacaagctat tcaaaaataa
     5761 atgcataaaa caaaaaactg cttgcgacat atcagaagac tttaaatctt cttctgatat
     5821 tgtccaagca atttttattt tgaagtttgc ttaattctgt tctttagaaa caatgtttgt
     5881 tctttttccg gcaaaaagaa agctagataa tacgacaagt aaaaagaaag cgatgattcc
     5941 aactgtcaag gctatttgac cgtattctcc tagtttccca agtacaagtc ctggtactag
     6001 ataatcagta tcggcaaatg tagaagaagc gattttaaga tctcctaata ctggaaggat
     6061 aaataatggc agccaactaa tcaataagct attgaaagca gaacctaata ccgcccctct
     6121 tctaccgcca gtcgagttgc cgaagacacc agaagcagct ccgcagaaga aatgtcctac
     6181 taccccagga atgataactg tagtttgcaa gcctatcata gcaaacatgg atagtgttcc
     6241 tacaaagaag ctaacaaaaa agccaattaa tacagcatta ggtgcatatg gaaaaacgat
     6301 aggaacatct aatgctggtt tagaatcggg tacgagtttt ttagcaatcc cttggaatgc
     6361 tggaacgatt tctgccaaca ccattcgaac cccttgaagg acgataacaa acccagctgt
     6421 gaatgttcca gcttggatca gtgcaaagat tacagcgttg tctccattgc ttagttctct
     6481 ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca tatacgatcg acatcaagag
     6541 catgatcgat acagtagaat ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc
     6601 tgtagaacgg tctttgtttc caaaaaactt tccgatccat ccactagaag cataaccgat
     6661 atttccggta tgccctaacg caacttgatt gtttccagtg atgttcttca taaacggttg
     6721 acaaagagca ggcgtaatag ttaatagtgt tccttcgaag accccgccta ataagatcat
     6781 ccacaaattt gcagtaaacc ccgcgcttac taaaatgact gctgttagac aagaaacata
     6841 aagcattgct tgacctgtaa gaaaaatgaa tttatatctt gtgaatctag caagtaaaat
     6901 attcacgatc atccctacta gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc
     6961 aatcgccact actgcttcat tgctaggaac tactccttga gtatgcagag ctgtttggaa
     7021 catcgaagca aaaggggtta gtgaattact gattaaacca gcaccgccga tcagtactag
     7081 gaatccgaca aatgttttga tagttccttg gatcacttct gaaaatttct tcttctgaag
     7141 aacgagacca agcagtgcaa tcaaagaaac gaggatggag ggctgactga cgattgacac
     7201 aaacaaattc aacaatttat ccattgatag ttcctccctt gaatcatttt tattcttttg
     7261 acaagatctt cgttactttt tcttttactt cttcgctgtc aatgatcgat tgcaaaggta
     7321 cgagcttttc atcaggtata gaagttagcg tatctttcaa agaatgctca atgaagaaat
     7381 aatcagccat atcgctagat acacttccaa catccgtatg acttacttca aactcgtcca
     7441 catctgctcc aagatctgca agtgcttgtt tgatgttcat ctctaccata aaacttgttc
     7501 ctaatccact ttgacatact gcaaaaattt tcatatcatt cgtctccttt tcttatgatc
     7561 gctaatattt cttcttttgt tgatgcgtgt aataaatctt gaagattttg tttattagaa
     7621 agtagctttg ttaaatttgc caaagcccga agatgtgcct cattatcgat agcagctaaa
     7681 cagatgaaca aactaatggg atgggctgga ttatcaagca gatccacagg ttgttgaagt
     7741 ttcagcaaag acatccctac ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg
     7801 atgtgatcgc caatatggat aaacgcacca tactgctcca cgcgctcgat catagctttg
     7861 atataacttt cttctatttt attttctgat aatagcggct gtgcggcaaa tgtgattgct
     7921 tcttgccagt tttttggttg atccgataat tggatcattt cttctgttaa taattcagat
     7981 agcataggac gttgatcctc cttcttttct aaaagtttgt tcattttacg atttaatact
     8041 tgatagagct tttcttcgtc tacgccttct tttaattcga tataaggcag tagcgccgac
     8101 aacaattctt gggcagaagg cattgaaaat cctggtatca gccaatctct ttgtacttga
     8161 ttgatcagct gatttttttc caagtcagtc atgattggat gcagaacata gagagacttt
     8221 ttcttatcag taagtgtgag aggtacagta gaaaaaataa tatcgtaaga agtttcgtca
     8281 acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa agatcatcgg gaataattgc
     8341 tgaagttcac tttttaagat caatgaagag ctaatgccac ttggacagac gatcacggct
     8401 cttatttttc tatttctttc ttctgcgtcc gcttttctga tctctccgcc aaatagtata
     8461 gtgaaaaaac caatctcttc ttcagggatg ctcttcccta ttcgtctttc taacggaagt
     8521 aatgcttttc ttgtcatttc gtagatggaa gcatattgat gcttaatttg atcgatcatc
     8581 acattaggta aataaaaaga ataggaaata cgaaaatagg cagggaccaa gtgataaaag
     8641 acattcatca ataattcacg aaacgaagca aattctacgg ccatcaggaa ttccatgcga
     8701 tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg caggctcctg gacatctcct
     8761 tcaacgatcg atgctaagca aagagagaaa aatataatct ccgaattttg atgttcggaa
     8821 tagccaaggc cagtcaatac ttgtcgacta atgtcagtga ctgcaagaga agccaacagt
     8881 tcagcatctt ttaccagttc catggatgtt tttccaattc gaatggttgt caaactcaaa
     8941 taataagcta attcatccat tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt
     9001 ttatctagca aatctctaac tttggcgtaa taatcaatgg aaagggttgc ggttaaatac
     9061 gtgagcccaa atcttccagc aagtgactgc atcaaacgag cagtgcgatt ttttgcttct
     9121 cttcttaata ttgcttctga ccctagcagc ctaaaaccag ttttcctagt atacgacagc
     9181 tgacaatctt ccacttctaa ttcatttctt atctttttga tatctgacag gactgtccct
     9241 ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa
     9301 accatcaaat aaagaagatc tcttcgctct tgttcggtat acacagcttc aaatcgagta
     9361 atatccaaaa atttttctgc ccagtcgatt gctcggaatt tctttatacg gatacgggta
     9421 tcatccacat attcggcaaa aggagttaat tgtttgatcg cctcgcttgt atttacttcg
     9481 atgatctctg ctgctttttc tagactgatt tctcttggtg ttttcaggag ataactaatt
     9541 ttgtagttca cagcgctgtt cacctcctac ttatattcta gtatggtagc gcttttaaat
     9601 cgaggggtgt ttggtacaac ttacagttgg atttttttta cgactgtacc gaacgatttc
     9661 tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc tcctgctttt atgaattgat
     9721 ttcgtccgat caatgatgat catgtttatt ttttgaggtc agttcttcaa atttttcttt
     9781 gacatcttca accgcttttt cgatatcttt ttcaaagtga ttgggctcat taggctttgt
     9841 ttcctcttct acagaatttg aaccagataa agctgtttct ttattttctg tttcaccaga
     9901 gtttgtgcca gctaatgatt cttctttgtc ttctgtttca ccagaattag ttcctgctaa
     9961 agccacttct tcttctgttt taggggccat tgaacctctt aatcgtttgt gtttttcacc
    10021 gtcataattt gtcatgttgg ttcctcctag tagttctata ctttttcatc gtacaataag
    10081 acagtttatg cgtcaaaaaa caaacccgat tcttacaaat atgtcatatt tttccttctg
    10141 attcttgaat gcttttgatt ccttcttcca aaattttcat ttgtaagatt gtttcttcat
    10201 ctttgatgac tttttctgcg ccattaacta atgtctcgta aatcccttcg tagacacggc
    10261 tgtaatcacc aacctcggaa attacttttt cttcatgata actcccttgt tcatctatat
    10321 aggtcaatac accataatgt tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt
    10381 aaaacatttt cagatgttct tcttgtcgat cttttgtttg tttcacgaac atcccttttt
    10441 ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa ataactggat tttatcgaaa
    10501 ttttcatatt tccatagaaa aaatccagat cgaagtaatc attcattcgt cctttcccta
    10561 aaagttgacg tacatcatag ctgatatcat ctggttcgcc gaaataagaa agaacttgat
    10621 ctactgtatg acaagcatgt ccatataaat agctgttcgc agtagaaaat tcatgcgtat
    10681 tttctggtac ttccggtctg aaataatcat aatgcatttc tacttctaaa atatccccta
    10741 attttccgct ttcgattact ttttggactg tcaagaagtc agaatcgaac cgtctgtttt
    10801 ggtaacattg gataaataga cctttttctt ttgctaatgc aaataactct tctgcttcct
    10861 ttgacgtttc tgcaaaaggt ttctctacta gtacattctt accatgttcg agtgccattt
    10921 ttccatattc ataatgaaat tgactaggtg tagtgatgac aactaattgg atctcaggat
    10981 catcccaaag atcagacagc tgatcagtat attcgatgtc ttcgaatgtt ttccacactg
    11041 gttcagatgg ggaaaaaatt tttttgatat tgattttgtt cgtaagtttt cttgaaaaag
    11101 gcaaatgata gcgatttgta ctctttccat tgccaagata agcgattgta agcatgagtg
    11161 gtgcctcctt ttattgatgt tttcagtata tcctagaaaa gcgcagaaag tatttataat
    11221 agctattctg gtaaaaaagt tcagaaaaac acttgttatc attcggtttg gtgaaattaa
    11281 tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga accaagtgtt tatcagtaag
    11341 atagaatttg gaggtcagat catgcttatt gaagaaaaac taacaaaaca agagttgttt
    11401 accacgacag agaaacggat tgtcgattac atccgtagga atatcgaagc tgcagtctac
    11461 atgacgattg aagaattagc aaaagctacg tatacttccc attcagctat cattcgttta
    11521 tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt tcgagctggg gcgggaagtt
    11581 catcaattga ttgctcagtt caatcaaacg attgatccta attttccttt ttcaagtgaa
    11641 gatagcactc agcaaattgc aaagaaaatg gcggatttat cgatccagtc aataaaaaaa
    11701 gcacaattgc agattgaaca tgaagattta gatcaaatcg gtaaagtatt gaacaaggca
    11761 cagcgaatct ttctttttgc aaaaggtgat tctcaaatca cggcaaggaa attccagaat
    11821 aaaatggtca agttgaataa gtttttgatc atggctgaag aatacagtga ttcttcttgg
    11881 aatgcagcca atttgactag cgaggattgc gccattttta ttagctacag cggtcgcatc
    11941 catcattatg aacgaataat gacctatttg aagcatgtag aggcccctac cttgctgatt
    12001 accggaaatc agcattcaga aatggccaaa caggctgata tgtgtcttgt tatatcgcag
    12061 gaggaatatg attttgctaa agtagcgact ttctcctctc aaattgcctt cgattacgta
    12121 ttaaatacgt tattctcagt gatatattct caaaattttg aagagaatat ccgcaacttg
    12181 aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa
    12241 aagatcctca tttagtaata agaaagatag tgtttgatag gctctctttg atatcatcat
    12301 aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt ttgtttacgg aacgattcga
    12361 atagatatat tatagaaatg aggaatagct aacttctgtt tcttattttg aacgattgac
    12421 gaaaaagata tataagaagg aggaaaatca gtatacatgc agctaacaat ccatacagga
    12481 aaaatcgttt gttctcggat actttctgta cttctttttg aacttctttt gcatgataag
    12541 gaacccggtg ccctctgaca agtaagcgat gactgttgac catataaggt gtacaagtta
    12601 ggagtgtcac gaaatcctgc cctttttcga ttctcagatc ttttgtatca gtcggttcta
    12661 ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc attgatttcg atatagaaag
    12721 aatcacctaa agtcagtttc ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat
    12781 ggctggaaat aacggcatgt gtattttctc cccccgtagg ataagacgtt ccttccaata
    12841 atgaggcacc tttttctagc agcacagtat tcgtttcatc aaaaattggc aaatggacat
    12901 tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc aaaataggtt ctatcctttt
    12961 tcgcaggttc ttttttctta gtaaagggat ctgcacctgg atttccacct tcttttgcta
    13021 attgctggtt tttctttgtc atgttttctt ggattttagc catgacttct tcattttctt
    13081 tgaccgcttg ttgctgataa tgagagatga tctgctgatc cagataatta ttcagcgcgt
    13141 cgctaacaaa tggataagct agtatcccta ttcctgagag aagcaagaaa agcatcagca
    13201 gatctagaag tttccttttc tttggtttca tttgtgttct ccttgtgatg actgtttgat
    13261 aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa
    13321 gactaaatct gactgtgctt gcgtctagca aagtacagtc cagcaatcag tagaaggact
    13381 actcctgcac cgatatacac atagattccc ttaccgcctg ttgaaggaag tgtacctttg
    13441 tgtttatttg gtattttttc aggagaaatc aactgtcctg ctgttacata agattgttca
    13501 tcgatagtaa atgctacacg gtttgttaat ggaacatatt tttctggcgc tttcgtttct
    13561 tccagatagt acgtgccata ttttagacct gtcacatcga ttaaaccgtt acttgtggtt
    13621 gtaaaaaccg ttgctgattc tttcgccgat acccagctga cggcttttgt ggatgggtcg
    13681 atcgataaat atttcgctgt gtcactatct tgatcacgaa cgacgaattc tgctccagca
    13741 agtgtttggt ctgatgtaac gtcaccatct actttaacaa atcgtttgcc cccagtaacg
    13801 acatcgactg acggtggtgt ttgatcattt gtatgcccgt tatcgacatt tgcttggtta
    13861 gaaaatcctt tggttggatc tgctttttcg ttcaaatgca tatagtaaac gaatttcaat
    13921 gtaccgccag gagttaatga aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct
    13981 gtttgctcag tgacagaata atttactggg tcgatttctt tatttccatc atataaggca
    14041 taagcatacg ttcctgaaga atcattatca aatgttaaag cagcgtcatg agtatcgata
    14101 agtttgaatg ttgtgtactt gttttgcgtt ccttctttat cagcgatacc taatgggata
    14161 ttgacagaaa tctcatattg aattttttca ccgattgcga catcttttcc attcaattga
    14221 ggtgttgttt tatcaacttt agacgtatca tttttgatgg tcttttctac tggtgtttgg
    14281 ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt caatcatttc cgcattatca
    14341 ggagctttta cttcttctaa attatatttt ccaacttcta aatgattaac gatcaactgg
    14401 cctttttcaa tagatgcctc ggcaaagtca ttgttgccga tgtcataaga atgaccagta
    14461 atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt acaatccatc tgtgacactt
    14521 tggatgtatt ttttgacgct tggtgttcct tcttctttag aaataataaa ttctgctcca
    14581 tttagtgctt cgttttcggc agtaccgatt tttgtaactt tcaacgttcc atcattacag
    14641 actgtatttt tagggtagag atggatagta tctagttctt ctgtcccgta tttataagag
    14701 ccatctgctt gtttgatcat ctcatataca gggaaagcta aaaccatgtt tgcggcagct
    14761 gacactccgt cttttggttc ttctttgatc gtgtagactg catctttccc attttgtttt
    14821 ttaggtaaag ataaagtgac atttccatca gcatctgtcg ttcctgaagc aactggtgta
    14881 ccaggagtca aagactggac tgcttgtttt gctgcatcca cggacgctcc tttatctcgt
    14941 tgcgcataaa attcttgagt gacgttataa actgaaaatg aaatatcggc taatccttgg
    15001 tattgatcga attcgctcat ttctttcccg ctgttttgga ttaaaggatc gggtaaatca
    15061 gtcattttct ttttatgaag gatgacttga gctgctgtct cctctgcaaa cacttttttt
    15121 gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa ggactcctaa cataacgttt
    15181 atttttttat gatttttcat tttttcactc ctgttctttt tttgataaac aaccagattg
    15241 caaagcttgc gaataccata ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt
    15301 ttggaagaaa tggacggcta ttttcttttt tattgactac tttcaatata ctgtccgcct
    15361 ctttatcctc agaaagaaaa gttgaaatgg taaacggaat ttctgtttca cttaaaatat
    15421 aaccagaagg tgcttcgatc tcttttaatc gataacttcc tgttttcaac ccggaaattg
    15481 aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc tgattcgttc tttgtccact
    15541 tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt gtttttaata aggaatactg
    15601 cgccagctaa tcgtttatct ggtttatttg tctcgatttt gacaaattgc ttactccctg
    15661 tatatacaga gactttcttt gtactgacac caaaaccaaa atccagtgtt ccgttgttgt
    15721 tgatttcctt gttcgcctgt gctgtactgc ttagctgcat ctgataagaa atggtcagtt
    15781 tttttccagc atatttttcc aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt
    15841 tcttgtgtat gacaaagcct tctgtcagct tttctccatt cgatgaaatc gttaaactat
    15901 taggtaaaaa cgttaatgct tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa
    15961 ggatatttac aggaattgtc gtatgcaagg aataagtgat tttttcacca atcgtaaaat
    16021 catttctagg ctcttctaat accttctcaa aaggtggatc agggtagtcg ttttcttcat
    16081 ttttaggata caaatggata gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa
    16141 caactaccat attctctgcc ttttctttga cgacttctgg tactttggat tcaatgaaaa
    16201 gatacgcttt atctcttttt tcttgatctt tagcggccag agaaaaagaa gcgataccat
    16261 cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt
    16321 tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc cttttcccgt agatggtaaa
    16381 aagaatctgt gacatcataa acttggaatg tgacaccatt taatcctcga tacgtttgta
    16441 ataaagcttt ttcttgtccg tcatttggat gatttttcgg cagttgcccg tttgggaaaa
    16501 gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt ttctgttgct acctgagtaa
    16561 aagctggttt aaatagtaac aagaagagac acatactaag ccaaccaagt tttttcatat
    16621 ttagctgccc cctctttctt ttttcaaaaa aagataccaa atagaaaacg cacaaccaat
    16681 catccctact agatagattc ctaaacggcc aattcctccc gtttcaggta atggtacttt
    16741 tgcctgattc gtgatgtcta aagaaatctg gttgtttttg gctcctggtg acagaacaga
    16801 ttcatgatct tgtccatcca cttgaattga cccatcttcg tgtataacta tagtaactgg
    16861 ctcttttaga ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat aagttccagg
    16921 ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg attctaagtc
    16981 catttctggt ccctgcagtc tgaattttgc tccttttaat gtctgtccgt tgtcttcttt
    17041 tttgtgtacc gttagatcaa acgcttttag tcgattttga tgagtgagcg tccatacatt
    17101 atttgtcatc tcggtatttt ttcccgtgta aatgattttt ccatatttat cgatttggaa
    17161 ttcataattt cccggcgaag tatcatatcc ggcaggacca gctgtttcaa cgactcgata
    17221 tttaccaggt gtgagtgaat caaaactggc aagacctttt tcattcgttg tttggctgtc
    17281 aattggctga taagtaccat ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag
    17341 atttatctct ttgtccgtcc cttgcatcgc atacttacgt actgctacag gaacttcaac
    17401 aaaaggattt tcaatagtca actggatcgt atcatcggaa gtagtgactg tttttccatc
    17461 aatggttacc gtaccatcag aagcgatctt gatttcccaa gtagtctttt tgcttaatcc
    17521 atgcccttct ggagcttttg tttctgtcag cgtataggtc atttcttttt gcaatttgac
    17581 attttctgga agagaatagg ttccgtcgcc atgatctgtt agtaaagtat caatagaatc
    17641 tcctgttaat ttgaaaacag cgccaataag atccttttca cctgtagagg aatttttcgt
    17701 tattttcaag tttaacggtg tgaattgttt agtatttttc catgtcattg catctatttg
    17761 ttgagaatcg tatcctggta caggtaattc tttgattgtt tggtaactga atgcttgacc
    17821 ttgattattg tattgaggta gtgataatat ctcttgataa ctttctccgc tatttgcaga
    17881 taatttgtca atgtctgcac gttcccacgt atttgttgta tctttagctg gtttaatgat
    17941 tcgaatatat ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa
    18001 agtaacttga tctggacgat cagctagatt gttgtcaaat tcttcccata actttttgat
    18061 gtgaagactg actccgggag ctttagcaga tggtataccg aattcagcta attcattggt
    18121 atcaatgttt ggctggaaag ttgtcctgcc gttcatttga taccagaaat ttggatggaa
    18181 gtcctcattt tctgtttgga ttctcacttg gtaatggatt tggatttctt ggtcttttcc
    18241 taaatagatc tgattgctct tgatggtatt tccttctatg gaaatagatg gagtgaccgt
    18301 tgtaggactt gtccccacac tcttgactga aagtgtacca ggctgataaa tgaatggttc
    18361 tgcgattgga tcatttattt gtccattgct tacagttgct gagatctgta cagctttttt
    18421 ggctaggtat tcagagatat ctgttgcatg atcagctgat tcatagtaaa gatcgccttt
    18481 ttcatctgat gaaaccattt gtcgcatacg agactctact tctgcttttg agagaccagc
    18541 tgccggatcg ctttgaagtt ggatgccaag accatgtatt tcgattcctc gttctttgag
    18601 tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg attcttctgg atagattgtt
    18661 ttggtcaggt gcatcataga ttcttgagat tagagaagta tttcccggcc gatcttgcgt
    18721 attagaaaac tgagttccgt aataattgct gcttgattgt gcgtgtaccc gctgtacttt
    18781 ataggaaaat gttggtacac catccgtcag caaaacgatc acttttttat gtccatttgg
    18841 aacggatagc atgcttcctg catctcttag tgctttttgt gtaaaagtac caccatttgt
    18901 ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa tcaaatgaac ccatctgtac
    18961 tgcaccgtta ctgtagctat atccttcgct tgagtagccg acatatccca tattgatttt
    19021 gtctgtgata ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa tctttacttc
    19081 accgatccga ttattgtcgt tcatacttcc tgaccagtct acgaccaata cgagatcaag
    19141 aggcgtgata tctttttgta catttccacg agcattcaaa taaacatcaa acaatccggg
    19201 tgtacttgtt tcctttgcat actttcgtat cgcatagtct gcttgattac cggttcctcc
    19261 atattcgata tacgaatggg tccgatcatt aggatctcca ttccaagaat ttataccatc
    19321 ccattgattt gttccgtttt tattgccttg atgatcaaga acatttgtat ttcctgtagg
    19381 ttgccaactg gctttaggat aagtgccctg atcatctgtt gtatattctg taggaatcgt
    19441 ttgattagat ggcacactca cagctggcaa ggataatgta ggcaatgtaa atgggctgac
    19501 agaattttcg ttttcttttg atgattcttc ttctatttcg tctggctttg gtactgacac
    19561 atctgtatct ttcttatttt ctgtctcatc aataatgtca tattctttat tatcgacgat
    19621 cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt
    19681 ttgtttagtg tttgatgaga tttccaacgt aattttcccg ctatttacct gataagcata
    19741 gccatctcct tcttttcttt ccacatcact taagtaattt tcgactagac caatttcttt
    19801 ttctatcttt tcagcactgg tgttattcgc atttagcttg atgttcagtg ctttttttcc
    19861 agactgatcc gaaatgactt cagctgaaat tgtttctaaa actgttggat catctgtagt
    19921 gtctgctatt gcagataatg gcacaaagtt tgataaactc aatattatca gcatgaaaat
    19981 aactttcagt ttcttccctg tggtcgtcat ttttcccctc ctttttcttt ttttctgttt
    20041 tgacgtgaat agtgaaaatt attaaaataa acaggcgtta cataatttta tttattttaa
    20101 tatgataatt attatacgaa gggcgctaaa gggaatcaca ccaattttca taccaattgg
    20161 tttcatttat gaagttgatt ccttcgatta atgagcgttt cattgaaaga aaagggttga
    20221 gggagaaaaa catatgatca ggttgttgga atgcaactat agaaagatgg aaaaaatcat
    20281 tcgaattctg tccatttctg acaagtggat gactaaaaaa gaacttgcca aacgaattgg
    20341 tagttcagaa tctacatttg taaggtatat cgaagagatc aaaatccgct ggggaacgga
    20401 tgtaacgatt caatcttcac aaaaattggg gtatcgtctt gaacatttga atgtaccagc
    20461 ttatttaaat gttctgatgg aaatgtcgca aagttctaca aacagtcgtt tattaaatga
    20521 gattattgtc aatccgggta aaacgattga gtattattgt gaaaaaattt ccatcagccg
    20581 gtcttctttt acacgtaagt tgaaacaatg caatcaaatt ttagaaagct atttattaaa
    20641 agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa agtgagctcc agttgcgtat
    20701 ctttgttacc tttttctttt taagttacta cggaagacgc acgttgccat ttccaatcga
    20761 aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt cagatgaatt tgtttcagga
    20821 agaaagccca tatgagcaat ctttctttat tatgtatttt gttgtgcatc tcatgagaga
    20881 atcccaagga actccatttg aatggaaagc tggagactct tatgtacagt tgataaatat
    20941 cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga aaattgaagt acagcagcta
    21001 tataaaagta gtgaactatt ttgtcaatac gattttttca gaattttcgg agaaagaaaa
    21061 aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac tgttcgtttt catccgctga
    21121 ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta acgcaaattt attttttctc
    21181 ccgtattttc ccatacgata cgactcattt gacgatccgt gcaacttact ttgcacagca
    21241 gatgcaacac cgccagatac gtctatatgc tggatttcgt gcagaacttc aattttattc
    21301 cactctactg aatcagaatc tgatttatta tttatctagc tatatctttt ggatgcttat
    21361 cggtaatcct gaaattcacc attatacttc tgataaaaaa atactgatta tcagcgactt
    21421 aagtcttcga catagtcaat atattgaaga atatatcagt gatattcttg ctgtgcataa
    21481 aattcactct gagacaactg ctattactga agatcagctg tcgaaataca atcttctcga
    21541 gtatgatctg atcgttacga atcaacccat tttgaatgcc catgttcctc atatattgat
    21601 tgatgatagt gtgtcattcg caaatgaaga agaactcatc cgcttattcg agttatgaat
    21661 aatcaaaaaa cagctatgta taaaccaccc tgataaaaat ttcaggacaa atacacatag
    21721 ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga tttatagcga aagtctttta
    21781 acaactcttc ttttgtccaa ttatttgcaa tgagatccgt caaagtgttc attacgaggg
    21841 tcatggaaaa gtataggaaa ctaactgctt cttccgacga ttgaatggag aaatgattga
    21901 ttcttaataa ttccagccat ttttcgttca ttggagaatc agccgatagt tctctacgtt
    21961 tggttgtctg ctgatcattt cgctgggtta acaagtataa cctttgccaa tagatacttt
    22021 cgtgatctaa gtcgcagcac cactctaaat atttttcgat ccccaaaaag aaatgttgtt
    22081 tgttttcgct aatgaatttt aagatatctt gatggactat gtgagaactg cttttgatcg
    22141 tatacaaata agcatcttcc aaatcttgaa aatatttata aaaagcaccg cgcgacatcc
    22201 ccatcttgtc aacgatttct gagactttga cttggctgat gtgctgtgag caaaatatct
    22261 ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga taaattgaaa aaagtttctt
    22321 ttggcatcca catctctcct ttatctctga ttatacgtgg atagcccttc attgtcatga
    22381 aggattcatt ccttcttttt ctcttcttct gtcaagtacc catcttccat tcgataaacg
    22441 cgatcactcc attgaatcat acggacatca tgagtaacca taatagtcgc tttttgtttc
    22501 tcatgagctt ctttaactag taatttgacg acttcatatg catgatctgt atccaaacta
    22561 gctgttggtt cgtctgctaa gatcaagctt ggctcattat ataaagcgcg agcaattgcc
    22621 actctctgtc tttctccacc agaaaggtct ttaggataac tatttttaag tgactggata
    22681 tctaaagatt gcagcaagga atcgattttt gttgaatctt ctttttttga agtaacttgg
    22741 tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga
    22801 ataaatccta tttctttaaa gcgtaaatct gctcgttttt tttctggaag ctcagttagt
    22861 gagtgaccgt tgatcactat ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta
    22921 aggaaagtac ttttacctga accagaagga ccaatgatgc tgacgaattc tccttccttt
    22981 acataaaaat caattgcttt caaagctgag acctttgtat gaccagaacc aaatgttttg
    23041 gtaacttttt tcatcgttaa tatttttttc atttttatta acctcctatt gctgtaattg
    23101 ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc tagcattgcg acaatgatca
    23161 aaatcccact atatagcaac cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta
    23221 aaatcagact agtcaaataa gcaaacaaaa gagcaagcag aacgcctacc accccaacaa
    23281 taaaggactg tgctactaaa gatttagcga tgaagaaatt tttgatccct tgtgctttca
    23341 ttactccaaa aatagcagtc ttctgaagtg tcatcacgta catgaaaata ccgaccacgg
    23401 cagcaaccac taagaacaaa aagtatatca tcgcatttaa ggtcatattt tgggcagaat
    23461 agccaggaat actttcaatg aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt
    23521 tttctttcga aattttagct gaatccttta ttacgatccc attgatcggc tgttcttctt
    23581 cagaagcaaa cggctgttgt ccatacttca aagcagtcca cgtatctagg gacgcataga
    23641 tgacgggaga aactgtgtaa taagtttcag gaaagatacc gacaatcgtc aaaggatcgt
    23701 cgtaactgcc aattttgatc gtatctccaa ttttatagcc tgaatcagcc agattttgag
    23761 agatgatgat ttcattcttt gttttaaatt catgcccttt cgtgagtttt ggtaataaaa
    23821 atgcttcttt cgttgtgccg aaaactgtaa cattttcttt gttttttcct ttgattgctc
    23881 cgctgtacaa tccgattggt gctttctctc ctccttcaat gtatttcaga tctcctcttg
    23941 ttaattgtga agctgcaaat acttgatttg catcctccga caaaacaatc tcttgtgcgt
    24001 cccaatcatc gattgctttt ttaaactctt ctgaaagtcc attcgctagt cctgacaaca
    24061 tgaatacgac atacgcaatc aagaacataa taccgacgat caagccatat cgtaatttag
    24121 aatagcgaat ttctttgata gctagaaaca tttgtttttc ctccctatga tagattgata
    24181 gtgacaaaat gtcacttgtt gaatagtgta actctaaagt gacaatttgt cacttgtgtt
    24241 gtttttttct tatcaataaa ttgtctaaga catctttcga taaaacctta acatgagtca
    24301 taaaaaattt atttaagcct tatttatgct ttccatgata aactaaagtg accaaatcaa
    24361 tgaggtggga aaatgattaa actattagta gtagaagacg ataaagtatt atccaataat
    24421 ataaaagaaa tactcgctgc aatcggcgag ataacacaag tttatgatgg tagtgaagga
    24481 ctgttcgagt tggaaagtgg agtttatgat cttgcaatct tggatttgat gatgccggaa
    24541 atgaatggat atgaagtgtt agcaaaaata cggaaagaaa aaatacagac tccagtattg
    24601 attttgactg caaaagatag tttggaagat aagattgaag gatttgaacg aggtgcagat
    24661 gattatctaa caaaaccttt ctatcgagaa gaactgatca tgcgcgtgaa agcattattg
    24721 aaacgctcgc taggtctctt tgatgaaaat gttctgaagt ataaggatat cagttgtgat
    24781 ttaaaaagca atgaagtctt gtataaagaa caagttttgc ccatacaagg aaaagagttt
    24841 gaattgctcg tttatttttt acaaaataag ggaatcattt tgacaaaaga gcagatattt
    24901 gaccggatct ggggatttga ttctgatacg acgattacag ttattgaagt ttatatgagc
    24961 aacttacgaa aacatctgcg aagtactgga ttggataaag aaatcaaaac cttacgtaat
    25021 gtcggttata tactgcaaag ggtgtgatgc aatgaatgga gatattacac gaaagcaacg
    25081 tagtcgcttt tttgcgacaa atgtagcagc atttgctttt atcttttttc ttttgggatt
    25141 cattacttta cagatattaa accaatctgc atacagagaa acagatctgt cattagagaa
    25201 aataaatttt gattctcgaa cagtacaatt agagatacaa agatatcagc aaggcgatcc
    25261 ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac atggaacagc cattaatgga
    25321 accatcaaat gaccgtttta acactcaagt gatcttgtgg tctaaaaatg gtgagatatt
    25381 gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa
    25441 aaatctgaat accatccaaa atattgaatt aaatgaagac agtggctcat ctgccctctc
    25501 tttccgttcg attacgaaaa aagcggagac gaatcaagat ggcgttgcct acatccaaat
    25561 tttagaaaat acaaatcaaa tcaagaattc attagcgact tttcgcacga ttatgatttt
    25621 gtgtatgatt gttttttgga ttctctctat tggtatcagt tattacttgt ctagtctaag
    25681 catgcgccct attattcttt cttggagaag acaaaaagaa tttgttgaga atgcttctca
    25741 tgaattgcgc acaccgttaa cgattattca aaatagtttg gaacatcttt ttaccaagcc
    25801 taatcatact attattgaag aatccgaaag catcgctcaa gcattgtcag agacgaggcg
    25861 cctcactgga ttgacttcgg atttgctgac aatcgctcgg aatgattctg atcagcagct
    25921 gctttcaaag caaatgatca atacacaaga atatatcgaa caacttgtta aaccatttca
    25981 agagatggcc atcatagatg gaaaagagtt tattctccat aacgatgcaa cattagaagt
    26041 accaattgat gaaaaaaaaa tccatcaagt tttggttatt ttgctggata atgcattaaa
    26101 atatacgaat ccagaagata agatcatttt ggattcatct gttaaccata aagaatggct
    26161 gattcacgta aagaatactg gcccttctat cagtgaagaa gatcaagcaa ggatttttga
    26221 tcgattttat cgggaagatc aatctcgggc gaaagagact ggcggatacg ggcttggctt
    26281 ggcaattgca aaacaggtaa tcgaacagca tcatggacag atttctgtat ccaattatct
    26341 tccacaagga gtcgatttta aagtccaatt gccgttgaag cctaaaaaat aatgcttatc
    26401 cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt ttttgttgcg aataaacggt
    26461 gttccagaag tagcttcttc ggaaataagc cgaaatttct ttaagctgac cacttctgtc
    26521 acaacctctt tagcttacgc ttacgatttt ttattgcggt cttcaatgtg tacatcaccg
    26581 cttgtgtcaa tatccaattc ttctcggcta acatcttctg tcactttttc aacatcttca
    26641 tgttcttctt tatgaatccc aacttcttca cgaataactg tatgtttgct tacatctact
    26701 tgctcttctt tgattgggat agtaatcgtg tcatcttcca atttttcgtc tgttccctgt
    26761 gaagattgat ctgttactgg tttgcgttcg atgacgattt cttcacgttt gaccggtact
    26821 tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt cgacttctcc agttgtcaca
    26881 tcatgtgtat ttacatctaa tttttcttct tttagttgaa ttgtatcatc atctagattt
    26941 ggtttgcgat cgatgtcttc agtcttgctt gtacccattg tatcagtctc gccaggtacg
    27001 tccatcggat cagttcctgg tgtcatattc aatggatccg ttgtattttg aaagcctgtc
    27061 gtgccaccta cgtccattgg atctgttcca gtgccagttt ctaatccagt ccccattgtg
    27121 cttgctgggc taacagtatc ttgtgtatcc attgtatctt ctgttgtttc tggctgacga
    27181 tagcctttta ctgtgatgac aaattttcca tcagaaatat ctcctctata ttgatataaa
    27241 ggatcatttt ctggtgtagc cgtctcagaa tcataggtat caaaagaaaa agtatctttt
    27301 attttttccc ataaagaacg gtcttcttca ccatccatcc gattttcatc tgtttcaaca
    27361 tctataccag acaatccgtc aaatcctagt ctagatgctg cttcatcagt agtataaata
    27421 acgatatcat ctcgttcata cccttcgtta cgcaaccgtt caattactga taatgcttct
    27481 tccctcgtac tatagcttcc gatgaccgtt aaatttttct cttccatgta aattcctcct
    27541 agcaaataga tttatgattg gtatattgtt acttataaat ttattctata ggagacgatt
    27601 gttttctaca aatatttaga ttggaatttg attaaaacaa tttaattgaa atttgaaaac
    27661 atttatgtta atgaaagtca tattctaata aaatgtcatt tgtttctata tgaattttta
    27721 tgaaaaattt catataattt ccactcgttg tgatggttta atttatccaa tccatcaata
    27781 agcaaacaac ttatataaaa aaggattaat tctcaatata attgctccta ccatagaata
    27841 aatcttcatg taaagtaatg aagtaccaac tatttctaat acttctttaa gaattatccc
    27901 aacaatatcg ttagacaaac cattattgaa taaatggaga gccaatcgat tctgaaaaaa
    27961 tgttttctaa tttttcggaa ataaaagaaa gagcctcctg ccattagaac gaaccaaatc
    28021 catttcattg ctcatgaagt aaccacatag ctaaaaagag gataaatcaa ttaaaagatc
    28081 aaaagaatga ctgcactaat aaaataacta aaaatgtatc ttcttacttc atatctttta
    28141 gctagtactt agttaaaaat ttgcttccta agatcaagac aaactgtcta tcttcatttg
    28201 atgaaatgcg gtaaaatata gaaaatgaac actattggag ggaatttgta atggacagca
    28261 aaactagaaa acaactgcaa gaaaagttaa agcaacgtac acacttgctc ccttccgtct
    28321 ttcaacagtt tttagaagaa aatcgttctt ccctttcatt aggcagtcgc tatgaatacg
    28381 caaaagattt tcatctgttt actgattatt tgagaaaagt attttctgaa gaagtggatg
    28441 atcagctgat tatccattta gaaaaacaag attatcagaa tttcttagct aaaatctatc
    28501 gacatactcg ttcgttccaa actacagcga accaagaaac ggagcaattg tttgaaaata
    28561 gctattacgg gatttcaagg aagcaatatt cattaaatca ttttttacga tttttgtatc
    28621 aaaaaggact gattgaagaa gagatttctc ttttgggtac aagtttaccg gaaacaacca
    28681 aagcagctcc gcgatatatc gatgcagaac ttttcaaaga ttttgagcat ttgtttatcc
    28741 cgattgaagg ctttcaaaca agcagagaag ctagctttga agaaaagaat agaccgagga
    28801 accttgcaat tactgccatt cttctgtatt gcggattaaa gatccatgaa gtagtagaac
    28861 ttaaacgtaa agatgtatct ttgaccaagc agattcttaa tctgaataga aaagaaaaca
    28921 gactgaataa agtaccaata ccaaaagaag cgatgccatt tctggagaac tattttaagt
    28981 taacagcaca aaatacggac gaaaattctt ttgtttttcg ttcctcacat gatcagcaaa
    29041 tttctcctcg gactattcgc caaattttga gcaaaattac agtatataaa aacgtatcgc
    29101 cagaactatg cagaaaaaca tatcgttatt atgcagaact atatttagat gatgctgacg
    29161 cggtaaatta tttatgtggt aatcgatacc tctcatccca ttcttttcaa gatatctggg
    29221 aaaagatggt tgatttttct tatcacgaat tagcggcatt cgttcgggtt tcttccatat
    29281 aaaacaaatg tatgatcttt ttttcacaat aaactatgtt attatataaa ggattatgtt
    29341 acgattatct tgaatttgta tattacttaa caaaagataa tggagtgtga atgatggaga
    29401 ccataacgta ttcccctttg aacttattta cgaattttca agaagctgca gctaactttt
    29461 ccgagacccc gattatcttt gatgagccac tttcagcttt cccagaatta ggacttgaaa
    29521 caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc ttatcaattg gctcatcttg
    29581 gagtgaaatc cggagacaaa gttattattt ataagagttc cgcttttgac acataccttc
    29641 tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat gacttcttat catctaccaa
    29701 caacaacaat ggaagtattt atcgatcgtt tagaagaccc tttcatttta tttgatgatg
    29761 agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa atcaaaacag attcctattc
    29821 tttatttaat ggaacaacct gctttgtctg tttctcaatc atttttagac aaaaatgaaa
    29881 tttcttatat gacccatact tctggtacaa ctggtatccc taaacttatt tgtcattctg
    29941 ctcattctat gggatggcgg acgaaatggc aaaaaacagt tttcacaaaa atttcggaaa
    30001 agaaattaat cgcttttcac atctctccag tgcattccag attcaatatt ggtatctctt
    30061 ctttgatgtc catgggattt cctatgatgc ctttagcaaa cgcacaaagt tcaaaagttg
    30121 ttcatatgtt ggaagctcac cggccaattg cactagagac ccatccgaat aactttgttc
    30181 agtggcgttt tactgctaag gagcatccag aagctttttc gggtatccga tactatcact
    30241 ccacttttga tgctataaac aaccagacaa tgaagacatt tttgcgaacg tcgatggcac
    30301 aggatgcggt atttcttcaa gtatatggcc aaagtgaatg cggaccaatg attcttaagg
    30361 ctcatacact agagagtttg aaaacaagtg atgcaagaga tatgggcgtt ggactggaag
    30421 acatgacaag cgctcggatt acagattcag ttgggaatgt cctacctgaa aacacagatg
    30481 gtcatataca attactgtct aaaggacggg ctttgactta ttacaaggaa gatgcacggt
    30541 tccaggaaaa tgtttatggc gattggtggg atagcggaga ttacggtttt atggataagc
    30601 atggccacct atttttgaaa gatcggcaag tggatttgat cgaaacgatc aatagtacat
    30661 tagctattga agacttttta ttagattcgt tagatttttt ggcagaagta gtgattgtaa
    30721 gggataaaaa tcattctcca caaccgatta ttgctttagc tcctgataaa gaaatggatt
    30781 ggaatcgatg gtgggagcaa gtccatgaac ttcctcgctt gaacgtccct atcatccgtg
    30841 attttgatgc aatcccgcga acagctacaa tgaaagtgca acggcttcaa atcgagaaag
    30901 aattaaaatc acaaaagtcc taactattta aggaaagaaa gatccatttt ggtctttctt
    30961 ttctttttgt ttttttaaat tggatgagat aaaaatatgc caattggctg tttttctttt
    31021 ttgaaaattt cgttatactc ttattcatgg aggaaaatgt tatgaaaaga atcttgacaa
    31081 ttggcggttc tgatccattt gctggtggag gaatccaaac agatttaaaa acttttgaga
    31141 attttggtct attcggacta agcgccttga cttctatagg ggcactagat attaatgagc
    31201 gatttatgct ggaatctata tctttggaat tgcttaaacg acagttagtt tcgattgata
    31261 aaatgacttc attagatggt atcaaaattg ggttattgaa ttcaagtgaa gcaatcatgg
    31321 ttgttcgaga cttcataaaa acaaaacaaa atatcccaat cattttagac cctgttttgg
    31381 cattcaaaga aactgaagct gtgatcaatc aattgtatat ttcgcagctc atcgaagaac
    31441 tttttccact tgttacgatg attacgccta atttggcaga agctgcttta ctttctggtc
    31501 aagctcttcc ctcttcgtta acagaaatga tccatctcgc taaagaactc actgcttcag
    31561 gaacaaaaaa cattgtgatc aaaggcggca caaggatggc tggagatgaa gctgtggatc
    31621 ttctctattc aattgatcat tatgaggtat tttctcataa aaaaatcgac actacaacag
    31681 taaacggggc cggttgtgcc ttttcttctg caataacagc gaatcttgtg ttaggggcag
    31741 attttaccaa agcagtagca gaaagtaaaa aatttgttgc ccattgtatt tctaatggcg
    31801 tgctgatgaa cgatcatacc ggaagtgtct ggtctggcgg tattcaaaaa ggaggaatga
    31861 gaaatgaaaa ctaagcaatt aacgatctat gctatgctga ttgcgttgac agttgccctt
    31921 tcattaacaa ttttgatccc tgtaccggct acgaatggat ttgtgacatt atgcgaggcc
    31981 gggatctata ccgctgcttt tctttatggt ccaacgggtg gattagttgt tggtgcttcc
    32041 agtggtctat tgatcgattt gatttcagga tatccgcagt gggcagtttt ttcattagtg
    32101 atccacgggt tacaaggata tgttgctggt tctttcagca aatcttctac tcgtatgtgg
    32161 ggattagggc ttttattagg aagcttagtt atgatcatcg gctatggtat cgccggttgg
    32221 attttatatg attggccggc tggagtggct tctataccag gaaacatctt ccaaaatctg
    32281 attggaatgg gacttgcctt tcctataact gcaagtcttc aacgtattca agcacggaag
    32341 caatattaat tgaaaaagaa ggttttagaa aatgaagttg tcagaaaaag aattaaaaga
    32401 ccaattgact gaaatagtta atgacatatt ggctgaggca cacttgaaaa aaggtgatct
    32461 ttttgtttta ggatgcagca caagcgaagt cgttggagga catattggaa aaaattccag
    32521 tgcggaagtt ggtcaatgga tcatccgtac gttaaaagag ttgttggatc ctaaagaaat
    32581 tgcgctagct gtccaaggtt gtgaacattt gaaccgagca ttagtagtag aacgaacagt
    32641 ggcagaagca aaaaatttcg aaattgtctc tgttgttcct gcactacatg ctggaggtgc
    32701 ttgttctgtt gctgcttttg accagttcaa tgatcctgtc gaagtggaac acgtagttgg
    32761 acaagccggt atcgatatcg gtgacacatc aatcggtatg catatcaagc atgtccaagt
    32821 acctgtcaga cctcgtttga aaacccttgg acaagctcac gtgacagccc ttcgctctcg
    32881 tcctaaatat atcggtggtc ctagagcgaa ttattgaaat gagtaattga cagagcaatc
    32941 tgaaaaactt cctcgcaaag tccaactaat gaactttaac gaggaagttt tacttgtttg
    33001 ttttatataa ccgagcttca taaggtgcta gtatgccttt agcttcaaat tctccgctag
    33061 aggaatttga caagagtaat gtccaggact gatctgcttc ttttggaagt tcatagtcag
    33121 caagtgattc tgttaagttt accataataa gatattgttc atcttctaaa tatctttcat
    33181 agacataaag ttgtggatgt tcatgaagat attctttata agaaccgtat atcagacctt
    33241 tattggtttg tctaatgcgg atcatgttct tatagaattg caggaccgac tctggttcat
    33301 attcttgttg ttctacattg atcgttttgg tatttggatt agtgatcagc caaggttcat
    33361 gtgtagaaaa accagcatat tgttccgaag tccattgcat gggtgtcctg ctgttgtctc
    33421 tcgttgtttc acgaatgata tccaatgctt cttctcttgt cttaccttcc gcaagcagcc
    33481 ttttataaag tcttttcgaa tctactgcat caacttgatc gatcgattca aagggcatat
    33541 tcgtcattcc gatttcctgt ccttgataaa taaatggcgt accttgcaaa aagaaataca
    33601 tcatagcgat tgcttttgca gacatagccc aaaaatcggg acgagtatct ccaaacactg
    33661 aaacggcacg aggaacatca tggttttcca tatataaagc gttccatccg ataccatcca
    33721 atgaagtttg ccaagcgctt aatgcatgtt tcaatttaag gacatctaat tgcccttctt
    33781 tttcttgctg ccagatatgg atatgatcaa attcgaatat catatcgaaa taaccgtctt
    33841 tccctaccca ttgtggtcct tcttctgcag tgacaccact tgcttctccg acagtcatga
    33901 tatcgtactc ttcaaaaaca tgtttcaaat cggtcaaatg ttcttcgatt cctgagacat
    33961 tttgaaaggg tgagaaaggg ttctctgtgg cttttactga atattcatct ttttttacat
    34021 gagaaatcgc atctaaacga aatccatcta tccctttatc caaccaccat cggatcatat
    34081 tgaatagttc ttctttcaat ttctcgcttt cccaatttaa gtctggttgt tcttttgcaa
    34141 aaacatgcag ataatactgt ccagtttcat gagaatactc ccaagcagaa ccgccgaaaa
    34201 tcgatgtcca attattcggt tctttgccat ctaccccgtc gacccaaatg taataatcgc
    34261 gatacgggct ttctttagat tttttcgatt cttcaaacca ttgatgctga tccgaactat
    34321 ggttgatgac aagatccata atcactttga tccctaattc atgtgcttct tttagcaata
    34381 aatcaaaatc ttccatcgtt ccgaatctct cttctatatc ttgatagtca cttacgtcat
    34441 aaccattatc gacaaatgga gattgataga tcggattgat ccagataaag ccgataccaa
    34501 gatctttcaa ataattaagt ttttcacgga tcccattgat gtctccttga ccatcgccat
    34561 tgctatcttt gaaactagca ggatagattt gatagcctac tgcttctttc caccattttt
    34621 gttcttgtat catatcgttt cactccttta acggtccctc ttgattataa ccgattctat
    34681 tttagaagta tccttgttac cggtaactag aaacgaaaag ctcaacgaat ctgctaagtg
    34741 cgttcgttga gctttatttt atcatagata gcgggcaatc agttgaatca tttgcttggg
    34801 aaattcattc aagtcagtaa tatctacaaa acgactttca ccgtagatcg acttgatttc
    34861 ttctttgtct tggccaatag ctgcggagac aaataggatt ccttgacgtt catattcttt
    34921 caaaacagac tggatatctt cttttgcttt tttgcctgta taatccggca atgcttttgg
    34981 ctgaccgtca ctaatattaa gcaacagttt ggttgttgcg gattgtctgt ttaatttttc
    35041 tgctgctaaa cgaagtgcag caccatcacg attgttttgc cttggtttca tagtgactaa
    35101 tttttcattt aaccattgaa atccgtcttc aaattcttta taagaaaaca aagaagtctt
    35161 ttctcttgac gaacggtctg ctgtatctcc gtaaatcaac aatgggatat cgactttctt
    35221 agaaaattcg gcaatggcaa tcgctgcttt ttttgccgct tcgatccgat catcccgtat
    35281 cattgaccca gattcatcga tccgtacagc taccgctaaa gaaggttgct catgtggcgg
    35341 gttctttttg tcaaaatttc tcaaatcacc atatgctact ttgcttgcat tgaaccgctg
    35401 accttcgtat tttccttttt ggtacgtctc tgtttgttca ttatccagta actctaaaat
    35461 ttgccggcta agtgattcta ctattggcat tacttgcatg tgtagctcac gtgcttccgg
    35521 cgtttttcct tggaaagctg gtcggtgaac gatgagacct tctttttcat gaatcgtttc
    35581 cttcatgatt tgtcgcgctt ctttattcag ttgcttcttg atttccttat catgtgcttc
    35641 catgtcagaa gcagacattt cttgagaatc atctcctgtt tgttgttctt tttgattttt
    35701 tccttcgata tcgctactgc gttctttttg catttgagga ggaatctcgt ttttttctat
    35761 tgtttcttct tgtttctctt ctccctcatt tggttcttca gcagacaacg atttcttttc
    35821 atctaagcgc agaatttctt cagcattttc agtgatgttt cgttcatttg cttcttcggc
    35881 gctaatggtt ctttcttttg tatgctcaga ttgaggatga ccccattttt cttttctcaa
    35941 tatgtcctga agttccagta gcaatccact gtcagacaaa gcttgttcaa gaattgccaa
    36001 ttcatctgaa tcagtggata atttatacaa aacttttgga taaatcgact ggagtaaatc
    36061 ttgtccttgc ttataggtat ttgcccaata aaaataagaa cgcattcctg ctacaccttt
    36121 gatggcgtta gcttttgcag tttcatccaa caaacggata atctctgcca tctttaagag
    36181 aagttcttgt ttttggatca tagttttcgc catcgcacgt tctgccatga cttctaaggc
    36241 aggaagatcc attttttccg catgttgcat ccgatctctt aaagattcat ttaatgggcg
    36301 atttccttgg taatttcgat ttgtagtgat cacaatgacg caatctggat ggcgacgaat
    36361 gattcctgtc ggtaaattca acatcccatt tggttcgagc gctgagttaa gtgccacaag
    36421 aacagaggca tctcggatga cagtcggctc ttgaatctct aataaatagc ctttttcaat
    36481 cgctcgaacg atttcagaag ggtaatagct gtaatggatt gcatctggat aatcagcttc
    36541 gatcctttga actagctctg ctaatttttc tttagcagaa aaaggatcaa tgccgtaatg
    36601 gttctggatc agtgtcagta cagcttcaag tgtatctgtt tggtaaatag cttctagtaa
    36661 ttcttggtct tgttccttat tagaatctac tacaggtaat aaggctccga aaacatctga
    36721 tttgtccata tctgcaaaac aggtcacttt tgtataggga agttgcagat cagcagatag
    36781 tgctttggct aattgtgtct tccccgaccc ggcatctcct tccaaaagta cgttactgat
    36841 tttcatttca ggatcttgcc aattcccttt gatttcggag gcgatccgcc attcttctgc
    36901 acttgtccga tggcttttcg gtttcttcca gatcatttgc ttttctagcc gtgaaaaggt
    36961 tcggttttct gttagtgaga aattctccat gcttctcttc cccttttgat catctctgtc
    37021 tattaatatc ctagctcatc taaaagacgt tttaaaatac ggaatcgttc cccaatcagt
    37081 tctccgtctt tttgtagtgt atcgtaatac aaaatgatgt cttcatcgat ctttggattt
    37141 ttggtatcta cttctaccgt cattcggttc ccttgtaatc caatgatgtc tacgactgga
    37201 ttttctggat cataaaattt ttcataacga taagcatctt ggaagtataa acttccttgc
    37261 gcagctaaga tcgctaaatc taaaatacgt gtgattggta attcttccga ctgtcgtgac
    37321 catttttctc cagtatgtcg ccataccttg cctgagatat cgacttttcc acgatcattc
    37381 cactgagcaa ggcccagtga aagccctttt gcatccatat ttaatgcatt tcttccatca
    37441 actttatcat agtcttctac aacgattact ggtttatgtt ttaaatttgt tggtatttcc
    37501 attattttaa ctcctcatcc ttttattttt agtaaataag taaattagta gtttactcat
    37561 ttactaaact atatgcgaca ttatagagga ttgaagtgag gattgcaaag aaaaagagct
    37621 tcattgatag aaataaaaac accatctcgt tcttttacac gctcattagt aacatgtaga
    37681 aagtccaaga tgatgttgga at
//
LOCUS       DACDYM010000022        36973 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDYM010000022 DACDYM010000000
VERSION     DACDYM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.022"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(129..557)
                     /locus_tag="IWM59_001093"
     CDS             complement(129..557)
                     /locus_tag="IWM59_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979140.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(585..1223)
                     /locus_tag="IWM59_001094"
     CDS             complement(585..1223)
                     /locus_tag="IWM59_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979141.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1227..3263)
                     /locus_tag="IWM59_001095"
     CDS             complement(1227..3263)
                     /locus_tag="IWM59_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9979142.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3389..4054
                     /locus_tag="IWM59_001096"
     CDS             3389..4054
                     /locus_tag="IWM59_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9979143.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5989..9138)
                     /locus_tag="IWM59_001097"
     CDS             complement(5989..9138)
                     /locus_tag="IWM59_001097"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP9979144.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9151..10350)
                     /locus_tag="IWM59_001098"
     CDS             complement(9151..10350)
                     /locus_tag="IWM59_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9979145.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10340..11935)
                     /locus_tag="IWM59_001099"
     CDS             complement(10340..11935)
                     /locus_tag="IWM59_001099"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9979146.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12519..13226)
                     /locus_tag="IWM59_001100"
     CDS             complement(12519..13226)
                     /locus_tag="IWM59_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979147.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13291..14127)
                     /locus_tag="IWM59_001101"
     CDS             complement(13291..14127)
                     /locus_tag="IWM59_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9979148.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14145..15554)
                     /locus_tag="IWM59_001102"
     CDS             complement(14145..15554)
                     /locus_tag="IWM59_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9979149.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15568..17391)
                     /locus_tag="IWM59_001103"
     CDS             complement(15568..17391)
                     /locus_tag="IWM59_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9979150.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18234..18560)
                     /locus_tag="IWM59_001104"
     CDS             complement(18234..18560)
                     /locus_tag="IWM59_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979151.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18606..18968)
                     /locus_tag="IWM59_001105"
     CDS             complement(18606..18968)
                     /locus_tag="IWM59_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9979152.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(19005..19220)
                     /locus_tag="IWM59_001106"
     CDS             complement(19005..19220)
                     /locus_tag="IWM59_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9979153.1"
                     /translation="MKKVNNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19347..19682
                     /locus_tag="IWM59_001107"
     CDS             19347..19682
                     /locus_tag="IWM59_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9979154.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19758..21755)
                     /locus_tag="IWM59_001108"
     CDS             complement(19758..21755)
                     /locus_tag="IWM59_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979155.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21853..22128)
                     /locus_tag="IWM59_001109"
     CDS             complement(21853..22128)
                     /locus_tag="IWM59_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9979156.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            22360..23094
                     /locus_tag="IWM59_001110"
     CDS             22360..23094
                     /locus_tag="IWM59_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9979157.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            23133..24275
                     /locus_tag="IWM59_001111"
     CDS             23133..24275
                     /locus_tag="IWM59_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9979158.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(24370..25761)
                     /gene="sufB"
                     /locus_tag="IWM59_001112"
     CDS             complement(24370..25761)
                     /gene="sufB"
                     /locus_tag="IWM59_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9979159.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25766..26236)
                     /locus_tag="IWM59_001113"
     CDS             complement(25766..26236)
                     /locus_tag="IWM59_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9979160.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(26223..27458)
                     /locus_tag="IWM59_001114"
     CDS             complement(26223..27458)
                     /locus_tag="IWM59_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9979161.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(27455..28741)
                     /gene="sufD"
                     /locus_tag="IWM59_001115"
     CDS             complement(27455..28741)
                     /gene="sufD"
                     /locus_tag="IWM59_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9979162.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28754..29527)
                     /gene="sufC"
                     /locus_tag="IWM59_001116"
     CDS             complement(28754..29527)
                     /gene="sufC"
                     /locus_tag="IWM59_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9979163.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(29724..30572)
                     /locus_tag="IWM59_001117"
     CDS             complement(29724..30572)
                     /locus_tag="IWM59_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9979164.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(30596..31282)
                     /locus_tag="IWM59_001118"
     CDS             complement(30596..31282)
                     /locus_tag="IWM59_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979165.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(31275..32309)
                     /locus_tag="IWM59_001119"
     CDS             complement(31275..32309)
                     /locus_tag="IWM59_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9979166.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(32704..33042)
                     /locus_tag="IWM59_001120"
     CDS             complement(32704..33042)
                     /locus_tag="IWM59_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9979167.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(33044..33400)
                     /locus_tag="IWM59_001121"
     CDS             complement(33044..33400)
                     /locus_tag="IWM59_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9979168.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(33406..34593)
                     /locus_tag="IWM59_001122"
     CDS             complement(33406..34593)
                     /locus_tag="IWM59_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9979169.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(34784..35629)
                     /locus_tag="IWM59_001123"
     CDS             complement(34784..35629)
                     /locus_tag="IWM59_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9979170.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35863..36828
                     /locus_tag="IWM59_001124"
     CDS             35863..36828
                     /locus_tag="IWM59_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9979171.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        10960 a         7582 c         6126 g        12305 t
ORIGIN      
        1 atttttttgt tgtttttatt atgatatatt tttatacaac taaaaatgaa tatattaaaa
       61 ctaccaaaaa taacttttaa cgctgtttca ttatatgttt tttctatttt atataaactt
      121 ttggatactt aataccaacc ggttggattg taccaacatg agaatgtgtc agttctattt
      181 cctgtcgcac ttgcataggc ccatactcct tttgttgcat ttgctgatcg aatagagttt
      241 ccaactttcg cttttgcacc gtgacgatta tagacacgac tatagtggtt agaatgacct
      301 gtttttctcg tacaagttgt atcactataa taaacccatc ttccagagct atgattaatt
      361 gctgttctag aaatttcccg gtctactggt aaatttgact taataatatt acctttagca
      421 tcaaaatgaa tctctcctgg ctttaaagat ttgtaataga tttcaccgtg aactgaattt
      481 gcttccactt cagatgacat agcgtttcct ccaacaaaac ctatccctag tgcacaaata
      541 ataactgctt ttttcataaa acgacatcct ttcctttttg atttttaaag catgatcttc
      601 ttgtttccta ttttggtcag atatggatca tgcgtgacta cgataactaa cgtaccttgg
      661 gaagcttgtt gcgataagat ttcaactact ttatccttat ttcttttatc caatgaccca
      721 gtcggttcat cggctaaaat gattttgcta ttcttcaata agactctcgc acaggcgatt
      781 ctttgcttct caccaccaga taatgtgtag ataggcgtac ttagcttctt gtttatattt
      841 acttttttca atgcctcata ctttgctgtt cgcttatctg aaacatttaa attcgaaaac
      901 ttcaatccaa tatctaaatt aaaatcgatt gtttcatatt caatcaaccc aaaatcttga
      961 aaaagaaaag atatggtatt tctttttatc aagcgcttct ctctcccagt taattgtttc
     1021 gtctcttgat cgaacaaata gaattgtcct gaactgaact gatcattcaa tcctaatata
     1081 ttcaataacg tacttttacc tgctccactt tctccaacaa cagtcacaag atccccagca
     1141 tccgcagtga aacgatccaa atgaagaatc gttttgtttc gaatgatttt tttcgcacct
     1201 tttatcacta tcaacggctc caattgttaa cctccattca tcagcaataa ctgacttttc
     1261 ttttctgtag acaaaataaa aacaactgaa agaataaaat caatgaagct aaagaaaaaa
     1321 atattaaaaa gatatttagg ctctctttct ataagaaaaa ggaagattac acttgtcgtt
     1381 ccccatttga tgaggttcat catgaagaaa tctttatacg tttcaaagag cgaataacca
     1441 cttgaacgaa agatagcgat tttccgacaa ttgtagacaa aatatatgca taacatgaca
     1501 tagctcaaca agaagaaaga aaagaaagtg agtaaaaata tctgtatcaa gtaatgaaat
     1561 gatctgctta acactttgac agctatctct tcagtctgag tatacggaaa tgataactgc
     1621 aagttatcac gtaactgact ttcttcaatc agctcaaata acctttcttt cgacaaagag
     1681 cctatcttga tctttaacgg tggatagatt tctccgctga gaatatttct ttccaaatac
     1741 atactgtttt tcaatgtcag tatttcaaca accggataat gttcgatcca ggggatagaa
     1801 ggaataaatg tataaatggg ttgattgttt tttataaata gaaatttcgg ttctttttca
     1861 ctgatttctt gataatattc tgttatttgc gccaggtcat tttttaaatc tgtatgtcta
     1921 tatcgttcag ggatcaatat aacagggtgc gtttcttttt cggttatttg gatcaacctt
     1981 ccagagacat caatcaaagg aaatttctta agataattca gatttatttt tatccctctg
     2041 ccaaactgtt cattttcttc attcaagtaa tctgatacat tgactaatat acttccgttt
     2101 ttattcaaag tttcatataa ttcctgatct gctgctgatg caaaacgatt ggaataaata
     2161 aaatccacgt ggtttttccc gatggttact ggataaaata catgatagtc ctcaccagcg
     2221 atttcttttg gtggttcatg ggaaacagct aactgtgaag aagaaaaaac gatagttgct
     2281 aattcgacat tggcaacggt caaaagaaga gaaaacaaca atccaaatgt aaaaagaatg
     2341 tattggatat agttgaacgc ttctcttaac gaagcgattt tttcaactaa taaaagagaa
     2401 atcaaagaga taccatatat tccgctgaac aagaaaacgt acatctggaa caaataaaga
     2461 aattgctcct tataatacat aaaaataaaa ccaccaatga ttcctgcaat tatgaatgta
     2521 ataatgattt tctttttccc caataaacac cacaatactt gatgtaatga aaagtcatta
     2581 ttcaacaata aactgatttc tttattcatc aataagaaaa aggatgataa caaaaggaat
     2641 aaaagaatca atccaatagt taaatacact cgataattat atacgtctgt atttgaaata
     2701 accgaatacg aatagctatt ttcaaagtct tcaaaaatat tagtggcata atcagtgtga
     2761 aatgcttgat tgaatcgatc agtgaatagt gaaatgaccc gttcataggc ttctttttct
     2821 ctcgtcatca agaaaaattc accttcaaaa tcttttattt tgatcgattc ttcaatggac
     2881 gttacgcgaa aggaggtatt agaatagggt accttctcgt gagaagattt gacatcccct
     2941 tgataataca atgtcatttc aagctttggc tgtatcttga agttccaaga tgtatctact
     3001 tcattggatt catttactgt ttttttcata taagaaagct cagtttctga tgagacattt
     3061 tcaaatacca tgattagctg ctcatattct tcttgcccaa taaaatgttg tggcaatcga
     3121 atcggagtat aagtatctgt ttgatggaaa aattcatttg tccgatagag tacctgttta
     3181 tttatactaa ccaaggcaaa accactcatg ataccggtca ctatgattgt gagtaaaact
     3241 gtgaactttt ttttgattat caccactctc ccttacttaa atgattcttt gataaaatca
     3301 taccgttttt catcaaaaaa acgtatctat caagtgtata ggtcatgtag aataaatcta
     3361 tatactgtag aatcgaaagg agaagaagat gaacaccgga gaaaaaatac gaacttaccg
     3421 cagaaattgt aagcttacgc aaaaagaatt aggcgaaaaa attggtgttt ccgataaaac
     3481 gatttctagt tgggaaaatt ctcgtacaat gccagacttt gaaatgctgt cacttttaca
     3541 tcatacttta ggtctgccga ttgattttcc ttctattgaa tcagctaccg aatcaataga
     3601 accaacattg ccagatgaac ctcttgttaa caagaaatct tattcatcaa aacaaccctt
     3661 ctcactcaaa aagacactat ctttctttct gctcgtcttt tgtttggtct atctttttct
     3721 tatcaattat caatacaaaa atgaaacgat cgatcgattc aatccttttt tgaaaccaca
     3781 aatcggctat accacactcc ctgtaggata cgtggacaat tccatcgaac gttgggtatg
     3841 taatgatatc tttggccatg gtacatggct gtcttttcac gctggagagg tagaaaaaga
     3901 gaaaaaccat gctgtaatca aacacaaagg aaaacacgtc atgaacgttc gcctgctcac
     3961 ttgggaagcc attccctcag attaccaaga aatcctcagc caagactatg acgacgcctt
     4021 aaaaagaagc cctcaggagc cagataggca ttaagtagga gatgaacgta gagatagaaa
     4081 aaatcaatgc acatgatctt atccaaaaaa caggggtaca aattaattga aaaagccttg
     4141 agatgaaagt tcctaaataa aaaagttcag aatgataggt atttctggct tatagtataa
     4201 tatttacgcc acagcctccc aatgattctt tttggaatca taatcaagga tggacattca
     4261 caaaaatatt ttgctcattc gatccgaata gattaacgac caatctagta agtaatatag
     4321 attggtacgt agtcaagctg cacataattt agatcaattt tagttctttt ttatatttct
     4381 atgatctgct catttacttt tatatgatgg cttacagtaa cataaataaa tatgataatc
     4441 gctggtagtt tatccaagag tactcaataa aatacaaaat ggattgttca agcgcctatc
     4501 ataatgataa aatgaaacaa ataaacgata caaggaggaa gagactttga gtaatggcag
     4561 aacaattttt tcattgtaaa aattgttcag ttgttaatct atgggatttc ggtagcatgg
     4621 gcattttttt atagaggaat cacaccaacc tggccgtttt atctcttgtt agtgggaata
     4681 ttagtaggaa attttgcccc tcttcactat cgtacgaact tttttccaac tgattctggc
     4741 ctttttctta aaacaaagac gcgaaaatta gaaaacatta ttgaaatgac aattacctgt
     4801 ataatcattt tcttagttat attcctaaac gtcttctaat aagaatctct taaaattagc
     4861 ttgttacagt tagttaccta gtttatataa cacagttatc ttatcttcct tatcaacatc
     4921 ttttgccaac tctttgtctt aaattcgaaa catataagca gagctacgaa caccaagact
     4981 gtttcggctc tgttttttgc atcaaattca ctcttatata gctgataatc tcggctcttt
     5041 aaaagaaatt attttcgctt cattttatta taaaaaggct aggaaccttg atgttatcag
     5101 gttcctagcc tttatttaag tattacccca cagacccttc catatagatt tttaaattac
     5161 acactagttt aaactaaaac aaacaaggaa aaacttacca tttaatcaaa aaattagctc
     5221 atatgaatat attagaatgt aaattagact ggtcaaaatc tggtcaaaaa agaggacgaa
     5281 tacaggtagt cctctttttt gttattttga tattctaaat cattttttat tggattattc
     5341 ccttgttcga tatagaccca aattgatcta tcaataatta tcaaactgag tcatatagag
     5401 acaaaaattt caaaaatgac ctgttaaatg gtcatcgaat atcatttaaa atcaatacga
     5461 cggagtcaga cacggagtca aaacaagccc gctgagagta aaaaatatac agtcccatag
     5521 gtaaagtgcc acaggactgt atattttttt atttgagatt acctgaaatg attccattta
     5581 atttttatct cattttttcc tactagttag tatcaaatac tctcctattt tctcaataaa
     5641 ctattttggg ttacatggta tctgaaacag ctaaaacctt gtcattatag caaaaaatca
     5701 ctttatctcc gtttcatttc agatacattt cacttaagcg gattccaaaa aaacaaaaga
     5761 aaaaggaacc cctagttagc ctatgctttt tattggttcc tctcaaaaca cggtcatata
     5821 acaacggggt ggggtaaatg aatgaaaaag gtggaggaaa cattattcaa tagctgtctt
     5881 aaatacttcg ttataaaatg tgaacaaata aaagacatct atccctattt tctacacttc
     5941 cacttgacta ccatctttga atcgtacaat tttcttaact cttctcgttt acctcaattc
     6001 attctcaagt ggaatcaact catcatctaa cattttacgc catgctcgct tcatttcttg
     6061 tgggtattta aatggcttcg cttctggatg tttggcttta tatccttcgt aatccttact
     6121 gttgataatt gctttcatat taggaattgg atcttcacct ggtagatatt gcagattaga
     6181 agcatgtaat tctttctcag aaacaaacca ctcattggaa aagacatgaa tcaaatcatc
     6241 actcgcttgt gtaaaatagc gacgtttaat gacaaagata tcttcattac tatctaaagt
     6301 ccccacatca aatgcattta aaatttgtcg gtaaacttct ttgactcctt ctgttttatt
     6361 caatttagcc aaagcttttt caatttcttc tcgtgcatct gaactattcg cagcataagt
     6421 tcctagtaat tgatcaatat aagttgaatc aatatcatac aacttagtgg tattttcact
     6481 ataaaattcg atcgttgaaa aatcttctcc ttctttttct ggttcttcct ctattaatga
     6541 acccttcacg gtctcgtaca cgccaatttg ttcttccaaa agttgaattt gattgttcaa
     6601 agttggagat ttcaccatgt catcattgta atcgtcataa gtcactaacg attcataagc
     6661 acgattcaat tcttgaaatg ctttgacgta ctcgatacgg atttcaagtg gcgtatgttc
     6721 atcaatggat tccatggatg aaacctcttt taacttacca tatgcttgtt tgaatctttt
     6781 tttggattcc tgataacttg gataaatcaa acctgattct tcttttgctt ttgaatacag
     6841 ttttgtcgca tcctcgacat tcttctccat tgtcgctggc ttacgaaacg tcacaatcaa
     6901 tccttttgtc ttatcaggat acgtacgatt cgtacgtgaa aaagcttgga tcaatcctgc
     6961 atactctaga ttacgatcca caaatagggt ttgaatcgta ggggcgtcaa aacctgttaa
     7021 tagacgatcc actacaatcg ctaaatcgat ttgtttccca aattgtttga attccgctct
     7081 ttttcgtgcc aaacgattat tgatgtctcc gttataacgc tcaatgtctg ctaatgacca
     7141 ggaagtatca taatactgat tatattcctc aataattttt tgcatttctt cttgttgggc
     7201 acttgaatct cggctatttt cttctaatga ataggtaata gcgattcgtg gaaaatcagg
     7261 atcctccatc gttcgtcctt cacgaatgag ctgattggga aactcttttg ttaaccaatc
     7321 aggctctttc gtcatctctt tgatggcccg ataatacttt ttggccatgt caatggaact
     7381 cgtggttaag attgcagatt tcgttggcct gccatttcga aaatcaaatt ttgtataggc
     7441 attatctgga cggaacattt tatgaatgac tttttggata tgttcatctg cttcaaaaac
     7501 agctgggtcc aaataacttt cttttttgac aggttccatt tgatcaatca tacgattgat
     7561 ctgttctgat gaatactctg catatgtttc tacttctttt aatttccgat agattttatt
     7621 ctctagtgaa gtcggctcca cggtgttttc atgctctact tgaaatccta agacagagcc
     7681 atcttctagc gcattcttaa tggtataggt atgcaagacc tctccatact gatcatgggt
     7741 tgtacgtgcc aattgccctt tggcttgttt ccgattctca ggaaaaatcg gtgtacctgt
     7801 aaagccaaac caagtggatt tcggaaacat ttttttgatt tcttccatat tttcagcact
     7861 taacgctcga tgacactcat ctactacaaa cacgatatgt tggcccatta atttttggaa
     7921 gcgattggtt ccttttttct cttcttgttt ctttgcatat ttcaacgccg catctagttt
     7981 ttgacgggtt gtaatgatta caacatttgc attggcatct gccaacaggg tttcacttaa
     8041 ttctttggca cttcccgttc caacgatcaa gctattcgct ttggcgttcc cggaagaaat
     8101 cccagtatta aattccgaag caaattttgt aaactctgtg gtggtttgat tatctaaatc
     8161 tttgcggtcc actaacatga tcgttcgatc gatccccgat tttcgtgcta aaagcttagt
     8221 agatacaaaa ctagtcaacg tctttcctga accagtcgca tgccacacat accctgattg
     8281 atgtttattt gcggcaataa acaaggcttg aatcgcatgt acttgataag gatgtaacac
     8341 catgagggtt ttattatctt gatcttcact gacgattgta taatccgcaa tcaaacgatg
     8401 agcatccggg atatttaaca cttgtttaca aaactcatat aagttttcta ctttttcatt
     8461 gtctttcgtt cgccaactaa ataaaaactt agggtgcaag tctttcggta aggcatttgc
     8521 aaaataccga gtggtttgtt cattggaaac cacaaacaac tgtaaggtcg aaaagatatt
     8581 gttccgaaac ataccttctt ctgcatattt tttgatttgg ttaaatgcct gataaacacc
     8641 atcttttgca gttacttgtt tcagctcgat ttggacaatc ggtaatccat tgattaataa
     8701 ggtgacgtca aaacgtcgat cccgtgcttc tatgttactt ccacgttttg caatttgatg
     8761 gaccacttca taagtagaga ttcctccacc aatgtcttga ttggaataaa ggataagtga
     8821 gacagaaccc aattgcgaat cttcccgttc aatcgtgatc cgagccatcc cattttcacc
     8881 ttttaaccat ttcgcagcat caaatggggt tttcgttcgc agcagtaatt ccgttttgat
     8941 cgtttcaaat tctttatcgg tcaaaggaaa atcattcagt tctgcttgat tatttgagat
     9001 aattttttgg cgtaaattga cccaaaggtc ttcttctgat tttagatccg gacgatacgt
     9061 ccactgatta tgcccttctc caagtacacg aatcaactgt tcttcgactt ctgcttcctc
     9121 acggtgacta attttcattc ttcttccctc ctagacaaac atcttttgta agaagctttt
     9181 tttcgtttct tttagtaaat ctaacttacg ttgatgaaga gcgatcgtat catctagctg
     9241 tttgaagaaa gtaccgattt tttgttgttc atcctttaca ggaaccttaa cactgatatt
     9301 ctcaatatca gaactgttat aagaaggcaa tgcccctact tgggagtatc ttggcaaatt
     9361 tatagtttca aacaatattt taccaaaata gatatcccaa gtattattaa ataaataagc
     9421 catggtattg ttatctatta agaaagggct tgtgaccaaa cgttttctat taagcattat
     9481 tgctgcgcca actttagcaa atataatcgc tggcacatct tttattggct tccatttttt
     9541 acgctcaatt tgctcattag atacataatg atttgcattt ctcatttcat actcattacc
     9601 aatgttattc atgtcggaaa ctttaaaaaa tggagtacct tcagaaccac cttgctcggc
     9661 atcaggaaat ccaattcccg attgagtttt acctaattct cccaacttac gctcttccca
     9721 atcttccgta aaaccaggaa accggatttc tggcactttt gctccgtttt cagggaacat
     9781 tttttgtaag aatccttttt tcgtttcctt cagcaaatct aacttacgtt gatgaagagc
     9841 gatagtgtcg tcgagttttt tgaagaatat gccgattttt tgttgttctt ttaaatcatt
     9901 gggaatatag aatactgtat ctgaaacttt tttccagtct gaacgaggca tttttgttcc
     9961 agatgtatca tttgcagcct tttgatatct atcagcttga ataagagaat aaataaagtc
    10021 cgagtcgctc ttcttagctc taaaaaccca gaaatcccca atcgctacgc ctgtaaaagt
    10081 tgccttgagc caatttttta aatatggacg taactttcca tatagaatat catttggtaa
    10141 aaattcaatc ccttttctat tatcaaattt ctggctcaca tctttattca gtctaccttc
    10201 tcctgccacg atatcttcaa attcaacttt tggaagtcta ctggagtttg tacttttatt
    10261 aagtctaaca actaaatcaa ttaacttacg ctcttcccaa tcttccgtaa atcctgggaa
    10321 acggatttct ggtcgtgtat cattgctcat gtttgaacac ctccagcgca ccttgtaacc
    10381 acatttcgtt ttcaggtgtt gtttgcaggg aagagatagc ttctaaaagt tctttttcta
    10441 gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc gacatgaacc atatcaattg
    10501 gttcttcttc ttcgaaagta tctacatagc gtgggatatt taagttatag tcattttctt
    10561 tgatttcatc aaaggttgcc aaatgagcgt atttctcgac atcttttctt tcagcgtagg
    10621 tttcgaggat tttttgaatg ttttcttccg atagtttatt ttgatttttc cctttaacaa
    10681 attcacgact agcatcaatg aataatacat ctcgagtttg acgattcttc ttcaacacaa
    10741 tcaccgtcgt tggaatcgat gtcccaaaga acaagttcgc aggcataccg atcacagcat
    10801 aaatactgcc atcttctagt aatttttgac ggatgacccc ttctgcagcg ccacggaata
    10861 aaactccgtg tggtaagacg atggccatgg tacctgtttc cttcaaatga tagaacccat
    10921 gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa tttcccataa cgattaaacc
    10981 gagaatcatc taagaaagtc gtatctgctg accaattagc cgaataaggt gggttcatta
    11041 ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc cttattcaac gtatccccat
    11101 tgcggagatt catttcttcg gcgtcgacac catgtaaaat caagttcatt tttgctagat
    11161 tgtatgtcgt tgtatttagt tcttgcccat gatatttaac gttatctggg tgtgttaagt
    11221 agttacgtac gtttaacatc aatgaacctg aacccatcgt tggatcaaaa acactaaaga
    11281 atcgtcgttc cttttgatcg agcgttacga tttgcgccat catatcagaa accatatgag
    11341 gtgtgtaaaa ttctccggct ttcttccctg cttctgaagc aaattgactg atcagaaact
    11401 catacgcatc cccgatcacg tccccatcgt gttctaaaac atctacatca ttcaatttct
    11461 tgatgacttc ggtaatcgtc acatttcgtt gttgctcatc tgttcctagt ttttttgatt
    11521 ggagatccac atcatcaaat aacccattaa attgattgta ggtagatgcc aattgcacaa
    11581 aggctttgtt tagatcgttc aattggaacg ttgcttgttt tgcttgatcc gctaagacgt
    11641 tgaaaagata ctctggtgca attgcatatc ccaaaatatc aacgattgtg gcaatcagat
    11701 cttcttttga ttcttcatct gaaagtaatt cacgatacaa cattgtttgt ttgcttactg
    11761 tgtcatattc ctctaaggat tcatctgcta aaagaaccac ttgttccaac aatcgatcgg
    11821 ataaatattt atagaaaatc agtcccaata aataattttt atattctgac gcatccattt
    11881 tactacggag attatctgcc gcactaaata atttttgatt taattctgca cccaaaaaga
    11941 tacactccta tctcttattt aaagttgcta tataccctct tattttagca tacccccttt
    12001 tataaaaagc aaaaaaaaaa gaaagaaact tattcttgct aataaactac gcgaaaggag
    12061 tctaacgttg tttgatataa cttagactga tcgttaactt attttttata tttctacata
    12121 gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa
    12181 aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat
    12241 cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt
    12301 catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag
    12361 cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat
    12421 aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca
    12481 gttacctgtc cgttcttaaa taccacagaa taatcttttt acccattttc aagtaaccga
    12541 agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct
    12601 aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt
    12661 cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat
    12721 gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat
    12781 gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg
    12841 gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt
    12901 cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat
    12961 ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct
    13021 acataataat cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct
    13081 gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc
    13141 aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc
    13201 tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt
    13261 tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag
    13321 ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat
    13381 tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc
    13441 atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt
    13501 cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc
    13561 tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc
    13621 ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa
    13681 ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt
    13741 tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact
    13801 gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt
    13861 aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc
    13921 cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa
    13981 tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc
    14041 aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact
    14101 gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt
    14161 cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta
    14221 agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg
    14281 gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt
    14341 aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta
    14401 attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata
    14461 ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact
    14521 acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct
    14581 tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg
    14641 ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag
    14701 gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag
    14761 ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc
    14821 aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata
    14881 gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga
    14941 attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa
    15001 ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc
    15061 tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca
    15121 tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa
    15181 taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca
    15241 tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc
    15301 ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc
    15361 tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct
    15421 tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc
    15481 tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc
    15541 tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt
    15601 taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat
    15661 cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg
    15721 tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt
    15781 cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat
    15841 cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt
    15901 ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt
    15961 cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc
    16021 tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat
    16081 aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt
    16141 ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa
    16201 accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg
    16261 ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt
    16321 gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc
    16381 aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc
    16441 agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt
    16501 aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc
    16561 gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat
    16621 gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt
    16681 aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt
    16741 aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa
    16801 agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc
    16861 aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag
    16921 tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact
    16981 actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc
    17041 acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga
    17101 aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt
    17161 ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc
    17221 gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct
    17281 tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc
    17341 ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc
    17401 tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag
    17461 tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc
    17521 caataatagc acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt
    17581 tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact
    17641 tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc
    17701 agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt
    17761 caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag
    17821 cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca
    17881 atagccaatt tcaatcattc cgagatataa aggtatcaat agaatgattt gggctagtat
    17941 tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca tgtagtaagt
    18001 atttcaattc caccaattat caataaaaat accaaacata tccataataa ttttttcttt
    18061 ttcatttctt ttatattgtt ataacagtga ataacataaa aattcccgac aatcattgta
    18121 atattttttt gtcacctgta ttagtattta atttatagga aactataaaa actccctagt
    18181 gaatttacac ctttgggagt tcctgtttgg acggtatgca taatacattt aaatcaattt
    18241 aacattaaca cctcagcata tggtatctcc acatatatac ggtctttctt tggatagtac
    18301 tcaatattta tgttgatagc acttccattg tctaatatag ctttagcccg gtatgtgtta
    18361 tctccatttt catgagtaat cgtcacatca tcacatgatc gatttaagtt gtactgttct
    18421 tgtaaaatct gtgttaccaa tggttttgat gacttctcta atccagatgt acctgtcatt
    18481 tttgaaaaaa taatacctat aatagccaag acaacaatac ctataatgac aaacttcttg
    18541 ttcatattta ctttattcaa ctttttatct cctatctcca aaataatttg ttcttatccc
    18601 tcttatcagt acattgacat ccctaaaagt acaccaaaga ttgctccagc tacccctgct
    18661 atcatcaaaa tcgtacccat tttctcatct ttttctcgat ataagtagcc cataataaca
    18721 gcaacagcac caaatacaac aggaataaat gctaacgata ctaccataga aatccaacca
    18781 ataatcaaat acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta
    18841 attgtctgct cagtactttt ctttactgtg tctgttccgg ttgtctcatc tgtttcttgt
    18901 agtgctccac aaaactggca atacttactt tcatctgtta tttctttgcc acattttcta
    18961 caatacatgt cttctcctcc taaaatcatt caattgctta ttagttaact gtttacttta
    19021 ttaaatacat cagaaaacag ctcattcatt ggtacactta atatctcaga aagcatcttc
    19081 atttgatagg gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta
    19141 acattcattt tttttgctaa ctccgtttga gaaagtcctt tttcttttct gtatcggctg
    19201 agattattta cttttttcat gattctcccc tcctgtttta ttatatacaa caatatgatg
    19261 tatttcaact cataaaatat aaaaaacttc aaataccatt acaaatacaa caacttgttg
    19321 tactatactg agatgggggt gtattaatga aacagaaaca atatgcaaag gtcattagag
    19381 aaaagcgaaa agctctcggg tggacacaaa aagaattagc tgaaaaaatc ttctctacac
    19441 aacaggcagt agcaagatgg gaaaattctg taacagagcc taacctcgat agcttaacag
    19501 ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt gtcgtagatt
    19561 atgaggaaga gtttttggct ctctatcgtt ctcttagcac tgaagatgca gttcgaacaa
    19621 ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt
    19681 gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa taagattaag
    19741 agctatggat aacctgtcta gtttaataca attgggtctt ttctcttcct tactctccgt
    19801 ccatcatctc ttgttacact tgtaaattga tacagaggaa ctatcccagc tcctgataat
    19861 atcaattctt ttattgtttc atctatccct gcccctttcg ctaaatatat taaaaaacta
    19921 ctacttgctt tcacattgta tagtttctta atatccttag aagctcgttc aagagtgaat
    19981 ccttgtttaa caaagtagcc tctgtttttt attaaagcat ttttcacgct gttatcccca
    20041 acattacgta ggcgttcaca aacagtgtta ataagttctt tctctgtcat tagatatgtc
    20101 ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc
    20161 ttgctgagtt ctgatcggga attttgagga aatactcttt tagcctcttt ttcgttaaaa
    20221 catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaatttt ttccagaagt
    20281 ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac gtctaatcga
    20341 tagacattgc ttctcttatg attaaagttt cctaacgctt tctcttcctt taacctattt
    20401 aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat caatcctata
    20461 aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt accatagtaa
    20521 gaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact ttccttacta
    20581 tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat ttcctctaga
    20641 taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc aaaaagtact
    20701 tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt ctctaatagc
    20761 caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac tccaataaaa
    20821 ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc agttttactg
    20881 gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa gtttttactt
    20941 gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc attaaaagac
    21001 atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc tttgtcgtca
    21061 ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct ccctgaatca
    21121 ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt aatatctaac
    21181 atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa aaacattcta
    21241 ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta ttcgtaaaca
    21301 gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat cttgtatttt
    21361 tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt atgaactaaa
    21421 ttgatagata aaaccttttc tatgcttaag tatctactat catctttact caaatctact
    21481 accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac tctggtttcc
    21541 acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa tgattgtgat
    21601 gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc taaacaccta
    21661 tctcttacat cagttttttc tttttttgta actggcttct ctaataattt ttcttgatct
    21721 acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt ataaaattct
    21781 gttaaagact aaaacataca gataataaaa acaaactctt tattactata tgttttagtc
    21841 ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag attttttaaa
    21901 tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat
    21961 cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat actctagagt
    22021 gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg
    22081 ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc
    22141 tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa
    22201 aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac
    22261 acataatacc tcgataaaaa aggggtgtca acagctttgt aaaatttatt tacaacaccc
    22321 cttttttgag gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata
    22381 aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa
    22441 aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaaa
    22501 actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt
    22561 gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt
    22621 ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa
    22681 aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg
    22741 ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt
    22801 aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc
    22861 atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa
    22921 gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat
    22981 cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca
    23041 tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa
    23101 gtactccttg taaactaaga ggagatgatt taatggcaaa aatatcaaag tacaaaaaga
    23161 aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg
    23221 aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg
    23281 acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg
    23341 aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg
    23401 caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat
    23461 cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa
    23521 gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat
    23581 atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa
    23641 atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg
    23701 gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg
    23761 aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata
    23821 aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt
    23881 ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac
    23941 agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag caacatgttt
    24001 ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata
    24061 ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta
    24121 gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa
    24181 atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag
    24241 gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt
    24301 tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt tctagctcct
    24361 ataacaggtt tacccaacac tgccttccat ctcataactg atcaatcgat tgagttcgac
    24421 tgcatattcc attggaagtt ctttggtgaa tggttcaaca aagcccatga cgatcatttc
    24481 agttgcttca gattcggtca ggcctcggct catcagatag tagagttgtt cttctgagat
    24541 ttttgagact ttggcttcgt gttctaaaga gacttggctg ttgtggatct cattgaatgg
    24601 gatcgtatcg gatttcgacc attcgtccat gatgatcgtg tcacattcga tatgagagat
    24661 cgaacccgca ctgtttttgg caaaggttac ttgtcctcga tagtttactt ctccgccgtc
    24721 ttttgaaatt gatttagaga cgatagagct agatgtattt ggtgcattgt ggatcatttt
    24781 tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg atggagagca tggtgcctcg
    24841 tgcacctttt ccatctaagt aaacacttgg gtatttcatc gttgtttttg cacctaggtt
    24901 tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt gctcgtttgg ttactaagtt
    24961 gtacacattg tctgaccagt tttggatcgt tgtgtaacgg caatacgcat cttttcttgt
    25021 aaagatttct acgatggcgg catgcaggct gttacttgaa taagtaggtg ccgtacaacc
    25081 ttcgacatag tggatgcttg ctccttcgtc aacaatgatc agcgtacgtt cgaattgccc
    25141 cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga acatcgactt tgacgccttt
    25201 tggcacgtaa ataaaggtcc cacctgacca tacggctgaa ttcagcgctg ctaatttgtt
    25261 gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat aattcagggt attctttcaa
    25321 agcagagtca gtgtcagtaa agacgattcc taacttttcg aattcatctt tcatattatg
    25381 gtacacgact tctgattcat attgtgcgga agcaccggct agataagctc gttcagcttc
    25441 tggtatacca atcttttcaa atgtatcttt gattttttct ggtacgtctt cccagtcacg
    25501 agcgggtttg tcacttggtt tttggtaata tttgatggca tcgaaatcga tgtctgaaag
    25561 gtcggggccc catgtttgca ttggcatttt gttaaatgtt tctagagatt tcagacggaa
    25621 ttcaagcatc cattctggtt cgcctttgat tcttgacatc tcacggatca cttcttctgt
    25681 caagccttct cctgtactga aaaccggttc gacatcatcg tgaaacccga atttatattc
    25741 ttctaattct ggtacgccca tgttctcact cctcttcctt gtggtaatga tggaaagctg
    25801 cggtaccatc gttttccatt gcttttccta gtgctttcca ggcaagtgtc gcacatttga
    25861 tccgagcggg aaatttagct acaccaccaa gcatagcagc atcgcctaat tggtcgggat
    25921 ctttgacttc ttttccttgg acaagctgta gaaagtctac agttaaatgt tctgcttctt
    25981 ctattgattt tccgatgacg gcgtctgtca tcatcgaggc acttgccgta ctgatggagc
    26041 agccactgcc gtcaaaggcg atatttttga tgattccgtc ttttacatcg acttgcaatt
    26101 cgatcacatc tccacatgtg gggttgttca attcgatctt gcttgtcgag tcagtcaagc
    26161 ttccacgatg atgcggatgg gaagaatgat ccaagatcac ttgacggtat aaattgtcta
    26221 atttagatag tcccatgttg gaaaaactcc tttgttgcca tgattgcttc aactaaacgg
    26281 tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc gagcagtcgc tgatacttca
    26341 aggtacttca atagcggttg cgcacaatga tgacctgctc tgacggctac cccttccatg
    26401 tctaaagctg tcgcgacatc atggggatgt aacccatcca aattgaatgc aacgactcct
    26461 gtccgaaaat tcgggtcttg tggaccataa acagtcaatc catcaattgc ttgtaatttt
    26521 ggcaacacat aagcggctag ttcttcttca tatcgatgga tattgtccat accgagtttt
    26581 tgcaaataat cgattgcatg gcctaatgcg atagcgccgg cgatatttgg tgtccctgct
    26641 tcgaatttcc aaggtagttc tttccatgta ctgtcgtata attcgacaaa atcgatcatt
    26701 tcgccaccaa attcaacagg ttccatttct tctaaccatt ttcgtttacc atataaaaca
    26761 ccaatacctg ttggtccgca cattttgtgt ccgctgaaag caaagaaatc acagtctagt
    26821 gcttggacat caatggccat gtgcggggca gattgagcac catctactac gatgaccccg
    26881 ccgttttcat ggacaagttc agctaattct tgaacaggat tgaagacacc taagacattg
    26941 gatacatgag caatggagac gattttggtt ttgtccgtaa tctttttcgc tgcatcctcc
    27001 atatccagga agccttcttc tgttagttcg atatatttca agacagcacc tgttcgtttt
    27061 gccaactgct gccacgggat aacattagaa tgatgttcca tgtaagagag cactatctcg
    27121 tctcctgctt tgatatagcg gtcgccgaag gttctggcta cccaattcaa gcttgtcgtc
    27181 gtaccacgag taaacagcac ttctgctgtt tcttttgcat ggatgaacgc ccgtactttt
    27241 tctcttgctt gttcataatc atgggtcgcg cgttctgcta acgtatggac accgcgatga
    27301 acattggcgt tatcgtgttc gtaatagtac gctaatgcct ggagtacttg ttttggcttt
    27361 tgggtcgtcg ctgcattatc cagataaact aaaggttcat cattgacgtc ttgaaataag
    27421 ataggaaaat cattgcgtag ctttttagca tcgatcatcc attcaacttc ccttcgatta
    27481 cttcgactaa ttctttccga acttgttcta cagggattgc tgttaagaca gaaccgagga
    27541 atcctcgaat cactaagcgc tctgcttctt ctttatgcaa tcctctactc atcagatagt
    27601 acatttcttc tggatcaact cgtcctacac tggcagcatg tccagcagtt acttcgtttt
    27661 catcgatcaa aagaatagga ttcgcatcac cacgtgcttt atcagaaagc atcaagacac
    27721 gactttcttg ttgggcatct gcaccttttg cccctttcaa gatgtggccg ataccattaa
    27781 aggtcaatgt tcctttttct cggatcactc cgtgttgcaa aatatgcccg atcgtatgag
    27841 gggctttatt ggttactcgt gtatcgattc cttggatttg tctgcccgag ctgattgcca
    27901 cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc tgagtcaaag tcagcgatca
    27961 catgaccgtc attcatgaca ccaattgccc agtcgacaga agcatctcgt aaaatgtgcc
    28021 cacggcgatt catataagaa gtaatatttt ctcctaattg atctacggct gaatatttga
    28081 tttttgaacc agctttagcg atgacttcca cgatgatatt acctgatgat tttgctactt
    28141 gttctttggt tgtctggaaa cgttccagat aagaaaattc actatgttca tctgccacga
    28201 tcaacacatg cttgaagaag tgctcatcgg atgcgccatc ttgaatgaat aaggactcga
    28261 gtggttcatc gatcacgaca tttttaggca catacaggaa aataccgctg ttcataaaag
    28321 ctacatggag tgcagtcaat tggtcttcgt tcatgtttac tgcttttttc atataatatt
    28381 cttggactag ttctggatat tcgatcattg cagtgaacaa gtcggtaaaa atgactcctt
    28441 tttctgctaa ttccacaggc agttgttcaa aaatcgtcca tgaaccttgt tggacaagca
    28501 caggattgtc tttcattgca tcaaatgccg gaatcgtgcc tgtttcactg atggtttgtg
    28561 tttctttcac atcaaataac gaccagcgat ggaatttcac ccgatcgata tgcggcaatg
    28621 gcagttcatc tgctttcgct agtgcgtctt gacggaaggt tctcatccaa tcaggttcgc
    28681 ctttacttaa agaaaaatca tttaccgcgt caagataatc gagccagttt ttttctttca
    28741 ttcttgctcc cccttacgct tcttcttctt tatagtcgat tcctaattct ttactgatac
    28801 cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc attccctgtc atgactactc
    28861 ggccttccat catgatgtga acgacatcag gtgtgatata gttcaataat cgttgatagt
    28921 gggtgatgat caatgcaccg aaattgtctc cgcgcatttc atttactcct ttagcaacga
    28981 ctttcaaagc atcgatatcc agacctgaat cgatttcgtc taaaattgca aatgtaggtt
    29041 ctaacatcaa caattgcagg atctcattcc gtttcttttc tccgccagaa aaaccttcgt
    29101 tcagataacg ttcagccatt tcttctggca tatttagtaa agccattttt tcatctaact
    29161 ttttcaaaaa tgccataaca gaaattttgt cgttttcatc acgtttcgca ttgatcgctg
    29221 cacgcatgaa ttccgcattc gtgatacctg gtatctcact tggatattgc atagctaaaa
    29281 ataagccaag tcgcgcacgt tcatctactt ccaagtctaa aacatttttc ccgtcaaaca
    29341 aaatttcgcc ttctgttact tcatagtttg gattgcccat gatagcggca gataaggtag
    29401 attttcctgt gccgttaggt cccatgatgg catggatttc tccggttttc attgttaggt
    29461 ttacgccttt aaggatttct ttttcctcga tcgacacatg tagatttttt atttctaaga
    29521 cagacatgca ttataccccc aaattctctt tcgttctcac tcattttaga ctaattgaga
    29581 atgaagcgca atgaccttcc ttactaaaat caaatatctt ttatttatta ttaaacaatt
    29641 ttgatttcta aattagaata ggtataaaaa agatgagaaa taagccatta cagacttatt
    29701 tctcatcttt tttgttccat accttatttt gcatcagcct ttactggttt aacagagccg
    29761 ccccatttgt ctgtgatcca ttcttggact tctttgcggt gaagcacttc aatcagtttt
    29821 ttgattttgt cactgtcttc atcaccttta cgagtcgcaa taatattcac gtaaggagag
    29881 ttgtcctttt caagcaatac ggcatcttct aatggattca accctgcgcc gtaagcgaag
    29941 tttgcgttga ttgcaactag gtcaccttca tcattttggt acgtagaagc taaaagtgat
    30001 gggtcgatcg tatggttgaa cttcaagttc ttagggtttt cttcaatatc atcgaatgtt
    30061 gctgtttcca gatctactcc atctttgact ttgactaagc cagcatcttg tagaattgtg
    30121 atgatgcgtc cccagtctga ttcagaattt gaagtaatga ttgttgcacc atcttttaat
    30181 tcggaaacat ctttgacttt tttagagtac aagcccattg gttcgatgtg gacagcaccg
    30241 gcattgacaa agtcatatcc tttttctttg atttctttgt tcaaataagg aatatgctgg
    30301 aagtaattgg catcaatgtc gccttcttcc aaagattggt tcggtaatac atagtcatcg
    30361 aatttgacga tttcaagatc gattccttca tctttcaata atggtttcac atgttccaaa
    30421 atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg tcgtgtcgtc tgctttattt
    30481 gctgaatctt tgctgtcgct gttgttaccg catgctgcta atcctacagt cagcaaaagc
    30541 gctgctgcaa atccaaaaat tttctttttc atatcctaca tccccctata tcctattatc
    30601 ttttgtctgt tttcttagtt aagaaatcac cgattccttg aacgataaat acgattagta
    30661 aaataattaa agtcgcaacg aatgtcactg ttagattacc acgctggtag ccttcctgcc
    30721 aagcaagacc tccaagtcca cctgcgccga tcgcgccggc catcgcagta aatccgatca
    30781 tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg aatactttca ggtatcaaga
    30841 ccttccagat gatttcccag taacttgctc ccattgcatc tgctgcttcc agtacaccag
    30901 ggctgacttc acgaaaagca atctctacta gacgggcata aaatggtgct gcggataaaa
    30961 caagagctgg aatggctgct tgcgcaccta gcatagtgcc tacgattgct ttagtaaatg
    31021 gaatgatcaa aaccatcagg atcataaatg gcgttgaccg gaaaatatta ctgatgatcg
    31081 atacgattcc ataaatgatt cgagcatacg gtttgttttt gcgtccaata gagtaaagga
    31141 tcaatcccaa aactaatccg atgatcgtca ctaagaccat tgagattaat gtcatatata
    31201 gtgtatcaag gatagcctga ttcatcgttt ccgggttgat ttgtttaaaa tctagatatg
    31261 tttctgaaaa gcttttatcc acgatggatc acctcaattc ctactccttg ttctttgatg
    31321 aacacttctg cttgatctaa ttctttggtt tcaccagtca tcaagacagt caatgaacca
    31381 aaggaacctt ctttcacttg tttgatcttt ccatacacaa cgttgacatc gatgttatag
    31441 cgtcgaatcg cttgggaaat gactggctcg ttggcatttc tttcgctaaa ggtcaaagtt
    31501 gccaaacggc cgcttggatt ttctttgatg agttcagcta atagttctgt cgaatcagct
    31561 tctggttcga tgtcttgttg tacaaagcgt ttggttactt cttttttcgg ttgtctgaag
    31621 acagtcaata cgtctccttc ttctacgact ttccccaact ccatcacagc gactttgttg
    31681 cagattttgc ggatcacatt catttcatgt gtgatcaaga cgatggtcaa attcaatttc
    31741 ttattgatcg ctaacaataa gtctaatact tcttctgttg tttgcgggtc caatgcgcta
    31801 gtagcctcat cacaaagcag gatctcagga tcatttgcca atgctcgtgc gataccgacg
    31861 cgttgtttct gtccgccgga aagttgtgaa ggatatgctt gtccgcgtcc ttctaacccg
    31921 actaaacgca acagttcttc tgcccgttct ttacgtttgc tctttggtac gccagctaat
    31981 tccagcggca gctggatatt ttcaagtaca gttcttgacc ataacaggtt gaaatgctgg
    32041 aagatcatac cgattttctt tcggaatgta cgtaattctt tgttttttaa ttttgtaata
    32101 tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct caagtccatt caggagacgg
    32161 actaatgtac tttttcctgc gccagaatag ccgacgattc cataaatatc ccctttttca
    32221 acgtttagtg aaacgtcatt gacggcttgg atgtttccgt ttttcccatt gaatgtcttt
    32281 ttgacattcg ttagttggat taatgacatg ccattcacat ctttctctta tgatttttca
    32341 aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac gaaagcttga acttccgtgt
    32401 taccaccttg atttaccgtt acttcgcaat aacagcctct tccagtactt ggacttagct
    32461 tctttccgta tactgtcgcg ctgtaacggg cactcccgta gtaagtttgc ttacgctcgc
    32521 ttactctgct ctaagaccat cttccatctc attttcgtta cccattttca gcaaacaggg
    32581 ctctcttcaa acgtccgtga catgtactct tcttttcaaa gctttctcgt ttcatatagg
    32641 attgcttatg attatagcaa ctggaagaat cagtgtcaac ttttttcaag tgattttctg
    32701 attttataat tcgtcaaatt cttttggatc aacatctttg aaacttaaga tccacgcatt
    32761 catttcatct gaatcattca agatattgat atcttcaccg attttatcat taacagaaga
    32821 aactgtaccc gttagcgggc tggcaaattc attgaccgct ttttccgctt caacttcgat
    32881 cagaggttca tcttttttta gcgattgtcc tgcttttggc agattggcaa acgtgatgtc
    32941 tccaagttct tcttgcgctt catttgtcaa accaacacag tattcttttc cattaaataa
    33001 gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc attttatatc agttctcctt
    33061 cataaatttt ttcattgaat ccaattgcaa caagctggtc ctctcgtaag atcagtggtc
    33121 gcttgatcag cataccatct gtcgataaga cttggctcgc ttttgaagcc gtaaactcgc
    33181 ctacttgatc tttcagtcca agttcccgat atttcatccc gcttgtgttg aacaggcgac
    33241 ggattggtac ttgactcgtt tctagccaag tctcaatcgt tgaggccttc ggtacttctt
    33301 tgatcatatc aacggtttga taactaacgc catgctggtc taaccacgct ttggcttttt
    33361 tgcatgttga acatttcggg taccagtaaa aggtgtacaa ttttcttacc tccttgaaga
    33421 tcttgcaggt gcttcatttt ggtatctcat cgataatatc aacccgatac cgatcatgtt
    33481 ccctaaaatc gaggatcctc cctgactaat gaaaggcaat gggatccctg ttaaaggaag
    33541 taatccgata tttgctccaa tattctcaaa gacatggaac agcagcatca tgatcaaacc
    33601 gcttgcgata taggcgtaaa attcattatt cgtatcgaaa cacacccgaa tcatacgata
    33661 gattaaaata aaatacaaga aaatgacgaa tgcaccaccg ataaagccga agttttcacc
    33721 gataacggta aaaatcatat cggactcacg aacaggcaca tagatattgc ttacgttgaa
    33781 tcctttacca aacaagccac cggtaccaat tgccaaaagt ccttgggccg gctgataact
    33841 cattcctgac gtatcatgga aagggtccag ccatgaatca atacgtgcaa actgataaga
    33901 tttgaatcca actttataaa gcaagtctct tccgccttca gtcgtaacca agaagatcgt
    33961 tcctgctcca acgactactg ccaagattac tacaggtgcg atgatgcgcc aagagattcc
    34021 agacatcagg aaaatcccgc caaagatcgc aagaaagaca agcatcgttc caaagtcatc
    34081 ttgggccatg atcaatgcta aaaccggtat tgtcacgatc aagagcttcc caatcagcca
    34141 aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta tgtttagtta cgatcagtgc
    34201 catcatcaag atgtacgcaa tcttcatcaa ctctgctggc tggaaagtaa acgcaccgaa
    34261 tcgaaaccag tttttagacc ctgttgaagc agctaaattc tgatcataga atttgagcaa
    34321 gagtcccatt accactaacc ccaaaccata aaggtaagga gtcagtttcc agatccattt
    34381 ggaattcaaa tgcatgatga caacgattgc tgccgctcca actaaatacc agacaacttg
    34441 catccctacg cctttcaaga ctgctggatt gttcggatca tggctaagtg ctacatataa
    34501 tgagagcaga ccaatcaaac ataacagaaa gactggtaaa atcacgccat aatcaatacg
    34561 attatcgttc gaagctttta attttttttc catgtttcat cccttttctt aaaaaccata
    34621 ctgctgatta ttatagcggt ccagttagat aatggaaagt ctttccatag acatttggga
    34681 aaaattaact ttccgtttgt gcagttgctt gagcaaatgc agtacaaaag cctactgctt
    34741 atctttagtg tatcatagaa ctttttttga tacatgcaat tatctaccag ctgagattct
    34801 cctcgctgtg agaagtttct gtgctttcat tgtatcgaag gatctcttcg gcaatctcta
    34861 gcagctgatc agaaaccaga ttggttcgtt ggatgaaata aaaatatcga tggaaattca
    34921 acggagtcat tggcacattt tctggaacag agcgcttaga caaaatcgag cgaccgattc
    34981 cttccttcag caacgcaaca attacatcgt tacttttcac gatcatcttc ttatcttgga
    35041 tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac accagagttt gcttctctta
    35101 caagccacaa tttgcttttt gggtccccag cgatgactag ctggtcttcc atcaaagagt
    35161 atctttgtac gccggaagtc tctaatggct tttcgataaa accaaaatcg gattcatgtt
    35221 tcgctacttg ttctaatgat tcatgagaat tcgtgatttc gatctcaaaa tgcactttag
    35281 gaaacctttt gatcagatgt ttcatcaact gaggcaaaag atagacagca aaggtgtggg
    35341 aagcaacgat ccgacatact tcttcttcat tttgttcatt gagcaatttt tgctgagttt
    35401 cttcccattc ttctaacaaa accagtgtcc gttcatataa taagtcggct tgcttagttg
    35461 gaagcatctc ttttcttccg ttacgttgaa aaagatcgat attcaattcc cgctccaact
    35521 gtttgatttg attcgagact gctggttgag ataaaaacag tacttctgct gctctggaaa
    35581 aatttcttgt ttcatatact gctcgaaaag tcgttaataa cttaaacatg gcaccacttc
    35641 cataactttt tatgataatt actataaaaa ctatttgatt taaatataca aaaaaccatc
    35701 tataatagaa atgtacaaca aaacagttgc tgatccaact agtaacttag atgaagacaa
    35761 tcagctgtct gcttgctcgt aatcatacat tcctattcat tttacaatgt attcgttcat
    35821 cttggtacta aaatctatta aataaaaagg agaatattat atatgaagaa ctttttgaaa
    35881 attattccga tcccgatctg cgggttgatc cttgggttag cttcccttgg aaatctgttt
    35941 aaagactatc accatgttgc tttaggcaat ctaactggcg gtatctctat gattcttatg
    36001 atattcattt ttgttaaatt gtttatctta ttcgaacaca cgaaacaaac gttgcatgat
    36061 ccaatcatcg cctccgtttc accaactttt acgatgtcac tcatggttat ttgtacttat
    36121 tttgtttcat atgaaagtat tgcaccaatt gtcaaattca tttggctgtg cgctgttatc
    36181 ttccaagtga tccttgtctt ttatttcaat tatcaccatg ttatcaaagc tgatctttca
    36241 attgaagcaa tttatccaag ctggtttatc atttacgttg gatttggggt catcactgta
    36301 actgccggga acttcttccc acttattgga aaaattttct tttggatttc attgatctgc
    36361 tatgcgttct tattgccgat catcatcaaa cgtgtctttt tcgtaagaaa tatggctcat
    36421 ccgacacgtc cgctgattac gatcgttgca gcacctggtt ctctttgcct aacaggttat
    36481 cttaaaaact ttgctgaacc taatttttat cttgtcgtag cattgttcgc tttatctcaa
    36541 gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt tcaagcttcg tttttatcca
    36601 agctatgcag cctttacatt cccgcttgtt atctctgcaa cagctatatt tactactgtt
    36661 cattacttca acagtttagg tatctatagt agcgtattgg atttcttaaa agttgcggaa
    36721 tgtctgtttg caagtgtagt tgtcttctac gttcttgctc attacgtacg tttcttaatt
    36781 aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa tcgcttaata atcgattcaa
    36841 taggttgtga aaaagctgtt ctgcatcaag taataagaac ggccaaacaa aaacagctgt
    36901 tcatgttttt gtactggttg ggcttattac cgcagatgca agcttttgaa caccgtttac
    36961 caagaggtcg taa
//
LOCUS       DACDYM010000023        35868 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDYM010000023 DACDYM010000000
VERSION     DACDYM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.023"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(5..1282)
                     /locus_tag="IWM59_001125"
     CDS             complement(5..1282)
                     /locus_tag="IWM59_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979172.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="IWM59_001126"
     CDS             complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="IWM59_001126"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9979173.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(2204..3148)
                     /locus_tag="IWM59_001127"
     CDS             complement(2204..3148)
                     /locus_tag="IWM59_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9979174.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(3150..3836)
                     /locus_tag="IWM59_001128"
     CDS             complement(3150..3836)
                     /locus_tag="IWM59_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9979175.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4310..4630
                     /locus_tag="IWM59_001129"
     CDS             4310..4630
                     /locus_tag="IWM59_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9979176.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4784..5689
                     /locus_tag="IWM59_001130"
     CDS             4784..5689
                     /locus_tag="IWM59_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9979177.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            5686..6459
                     /locus_tag="IWM59_001131"
     CDS             5686..6459
                     /locus_tag="IWM59_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9979178.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(6640..8064)
                     /locus_tag="IWM59_001132"
     CDS             complement(6640..8064)
                     /locus_tag="IWM59_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9979179.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            8275..8721
                     /gene="nrdI"
                     /locus_tag="IWM59_001133"
     CDS             8275..8721
                     /gene="nrdI"
                     /locus_tag="IWM59_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9979180.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(8786..10768)
                     /locus_tag="IWM59_001134"
     CDS             complement(8786..10768)
                     /locus_tag="IWM59_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9979181.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="IWM59_001135"
     CDS             complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="IWM59_001135"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9979182.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(11681..12742)
                     /locus_tag="IWM59_001136"
     CDS             complement(11681..12742)
                     /locus_tag="IWM59_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9979183.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(12953..13480)
                     /locus_tag="IWM59_001137"
     CDS             complement(12953..13480)
                     /locus_tag="IWM59_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9979184.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(13547..15049)
                     /locus_tag="IWM59_001138"
     CDS             complement(13547..15049)
                     /locus_tag="IWM59_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9979185.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(15307..16428)
                     /locus_tag="IWM59_001139"
     CDS             complement(15307..16428)
                     /locus_tag="IWM59_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9979186.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            16554..17495
                     /locus_tag="IWM59_001140"
     CDS             16554..17495
                     /locus_tag="IWM59_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9979187.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(17645..18541)
                     /locus_tag="IWM59_001141"
     CDS             complement(17645..18541)
                     /locus_tag="IWM59_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9979188.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            18810..20150
                     /locus_tag="IWM59_001142"
     CDS             18810..20150
                     /locus_tag="IWM59_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9979189.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            20368..20811
                     /locus_tag="IWM59_001143"
     CDS             20368..20811
                     /locus_tag="IWM59_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9979190.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(21315..22733)
                     /gene="nhaC"
                     /locus_tag="IWM59_001144"
     CDS             complement(21315..22733)
                     /gene="nhaC"
                     /locus_tag="IWM59_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9979191.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(23136..24359)
                     /locus_tag="IWM59_001145"
     CDS             complement(23136..24359)
                     /locus_tag="IWM59_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9979192.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(24390..25229)
                     /locus_tag="IWM59_001146"
     CDS             complement(24390..25229)
                     /locus_tag="IWM59_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9979193.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(25246..25926)
                     /locus_tag="IWM59_001147"
     CDS             complement(25246..25926)
                     /locus_tag="IWM59_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979194.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(25928..27001)
                     /locus_tag="IWM59_001148"
     CDS             complement(25928..27001)
                     /locus_tag="IWM59_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9979195.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(27648..28319)
                     /locus_tag="IWM59_001149"
     CDS             complement(27648..28319)
                     /locus_tag="IWM59_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9979196.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(28406..31084)
                     /gene="mgtA"
                     /locus_tag="IWM59_001150"
     CDS             complement(28406..31084)
                     /gene="mgtA"
                     /locus_tag="IWM59_001150"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9979197.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            31663..32100
                     /gene="ndk"
                     /locus_tag="IWM59_001151"
     CDS             31663..32100
                     /gene="ndk"
                     /locus_tag="IWM59_001151"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9979198.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(32122..32988)
                     /locus_tag="IWM59_001152"
     CDS             complement(32122..32988)
                     /locus_tag="IWM59_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9979199.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(33148..35241)
                     /locus_tag="IWM59_001153"
     CDS             complement(33148..35241)
                     /locus_tag="IWM59_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9979200.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        10383 a         7532 c         6332 g        11621 t
ORIGIN      
        1 ttttttatag taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt
       61 ctaacttctg ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc
      121 ctttccattt gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt
      181 gccatttttc ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt
      241 ctttacgcaa tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc
      301 cttctgtaaa accatagcga caatattgct tccaaataaa tagatagatt tttggttgga
      361 tgaatcgttg gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac
      421 gttttgtctt tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca
      481 cttcttcata tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga
      541 attcttctac atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct
      601 gtcgcttttt tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg
      661 cttgataatc cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag
      721 ctaattttat atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac
      781 agtatttttg ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt
      841 cgtctaaggc cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag
      901 agacatgcag gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg
      961 gcgatttaac gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct
     1021 ggcttcgttt tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg
     1081 cagctaaata atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga
     1141 ctagttcccg ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt
     1201 attgtttaga aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa
     1261 agacggattg ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta
     1321 ctaacataac gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat
     1381 ttccggaaat atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag
     1441 caatgtcttc taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg
     1501 agaatggcac gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa
     1561 gcaaacgata catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg
     1621 gtagaccatg atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag
     1681 cggctggacc gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga
     1741 ttcttgcttc tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg
     1801 cttgtccgac acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt
     1861 gactgctttt ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt
     1921 catctggcat tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat
     1981 caaaacctgt tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg
     2041 ttatctttta tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat
     2101 aacataacag atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta
     2161 gaagaggttg cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca
     2221 tataagcaat ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc
     2281 cgtatttgtc tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg
     2341 gtgtcagcag ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat
     2401 tcgctccatg actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg
     2461 caaatgcatt ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc
     2521 cgatgttcac atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa
     2581 ctccagcgct tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg
     2641 gccctactgt gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc
     2701 gagaaaagaa catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag
     2761 cacctaaatt ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa
     2821 taaaaatttt cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc
     2881 ggctttctcc tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc
     2941 caccgttcac gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc
     3001 agaaaaaggc tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag
     3061 tgtaaatcaa tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata
     3121 aaatatcagg atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc
     3181 gctaatttct tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc
     3241 actggaactt ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt
     3301 gttgattgga ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag
     3361 cagacatttt ccatagctga agccatacct tttagttctt cttcatgctt aggatggact
     3421 tctaaccctt ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat
     3481 ccagcaactc caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa
     3541 atatctacaa tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat
     3601 tctttcagac catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc
     3661 atcccgcgat tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga
     3721 ccgccagtca aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta
     3781 aatgcaaacc cgagaataac aatcaagacg cctaaaaggc taagataatt ttccatatat
     3841 tttcctccat aaaatgacaa tattttttta attataaagc aaaacacata aaaaatatag
     3901 ttgttttctc acgaaataga cactattttt aaatattttt cgttattttt ttatcacaaa
     3961 aattatctta cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga
     4021 aataaggtct agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa
     4081 attgcaaagc attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt
     4141 tactaaaaag tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata
     4201 tacaatctgc caccgggtag gataaagagg atgatcattc cattaggtct ttgaagggat
     4261 gattttcctc tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat
     4321 ggaattaatc atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga
     4381 tggtttttct aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta
     4441 ctttttgtca aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg
     4501 gattggcgca gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct
     4561 ttccacttgg ttctttgtgg ggatgctact catcagtatc attggcatca agattactag
     4621 cggccactag acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat
     4681 tcaatttttt tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat
     4741 caggtgtatg cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt
     4801 ccaagaatta tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt
     4861 atcccctggc aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat
     4921 gaaagctatt ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga
     4981 gatttctccc tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg
     5041 tatctatcca ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt
     5101 atctgctatc gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc
     5161 gaaatcctac tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca
     5221 tccgtctatc gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga
     5281 tgtgcgaaaa atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca
     5341 cttactcagc gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat
     5401 catcaaacaa tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc
     5461 tacaacaaat gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga
     5521 tcctgataaa atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct
     5581 attgtctgca caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc
     5641 ttctctattg gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgcttgta
     5701 aaacaagaat tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta
     5761 gttgttttgc tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca
     5821 gttgaaatga ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca
     5881 gctgcaagca cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat
     5941 cgaaagtatt ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct
     6001 gtttatttat accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct
     6061 tcgatttcct tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact
     6121 tatactttgg gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct
     6181 tgctttatca atagttccgg cgcatccatc tcagcaggaa tcgtctttta ttttgcttcg
     6241 tcaatcatat ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat
     6301 ccgatcagta tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg
     6361 actcatcttt ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta
     6421 gcattaggat atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct
     6481 gtgaaagaga cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga
     6541 aaaaggagct gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac
     6601 gacaggctca caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt
     6661 ggcaaataaa accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc
     6721 taagtggata tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat
     6781 gcctggcaag aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc
     6841 ccatacttga aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg
     6901 ataagaaagt ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt
     6961 accaagtgtg taagcaaagt tataagcact gtcagtgaat aataatgaaa gtaaaaagac
     7021 attcgtacat aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt
     7081 tccccatgct ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa
     7141 gatcgtccat gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac
     7201 tgctccccat ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat
     7261 cgtttgcagc tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat
     7321 aataatcaag ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc
     7381 tcgtgaacct acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat
     7441 gcaactcaat acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc
     7501 gctagaaaag ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa
     7561 taaaaataat ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc
     7621 gactccccga ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga
     7681 tgccagattt tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa
     7741 tgttgcaaaa gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa
     7801 accagcgaaa cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg
     7861 acgtttttta ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag
     7921 tacccaactg aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa
     7981 tacgccacct ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc
     8041 ttttttctgt tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa
     8101 aagtataaca aacctttttt atttttactt aattaataaa aactttaata gaaatataat
     8161 tctaccggct tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca
     8221 ccaaaaaact taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac
     8281 attttatata tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat
     8341 gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat
     8401 gaaaatagtg attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta
     8461 gacggcggaa atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa
     8521 tatctggaac atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa
     8581 aacttcaata atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct
     8641 ttcttggcag attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt
     8701 ttagcagaaa acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc
     8761 tttcgtttct attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt
     8821 ttgctttacc tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga
     8881 cgatcatgga tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa
     8941 gcgtatcagg tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta
     9001 agtcaactgt gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc
     9061 caatagcgtg tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat
     9121 gtccgttttc tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat
     9181 cacttgcttt atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac
     9241 tttcagctga agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag
     9301 tcgctaattc ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga
     9361 agccaccagc ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga
     9421 tcaatgggat caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca
     9481 ttccgatcac tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta
     9541 gaggtaatgt tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag
     9601 ctcctttgat catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg
     9661 ctgcacccac ttgtcctgca ccagccatcg caagaattgg aagcaatagc gtacgaccag
     9721 tttcatcaat catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca
     9781 tcggcaagaa taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc
     9841 agttgatccc accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa
     9901 tttcaatcag accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat
     9961 caggtactac tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga
    10021 cacctaggat cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg
    10081 gtgcttccgg attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg
    10141 gtgttgcacc aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca
    10201 tcccgttttt caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg
    10261 ttccggctgt gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga
    10321 caaatgcagg aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt
    10381 tagatggttt ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac
    10441 ggtcttttcc agatgctgat gaatttgctg tagctgtact tacagaagaa agtttttctc
    10501 ctaatttcac gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc
    10561 cgatgatgac ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta
    10621 attcagagac atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt
    10681 ggacaatgct ttctacattg cccatgccgc ctgccccttt ataaatttgt tttgccagtt
    10741 cttccaaagt cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca
    10801 aatccgtttg cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc
    10861 atgacgatag ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg
    10921 taatcagctt ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcattt
    10981 gttggtttga catcgaccat caagttattg aatactttac ctataccaat catactgata
    11041 gtagaaatca tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct
    11101 gtcaaaaatt ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg
    11161 gcttgtttat tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat
    11221 ccaccgatca catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt
    11281 gtcaaattca gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg
    11341 acagctacag tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct
    11401 acaccgaatg tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg
    11461 gctcccatat aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact
    11521 ggttcgatac ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc
    11581 attagttgaa cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg
    11641 cgttccgttg ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta
    11701 taaattgata gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa
    11761 ctttagctac tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct
    11821 cacccatata gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag
    11881 ctgtatttct tgctgggata ggcggaatct ctcgaaaacg tgcatctacg cttcgtatga
    11941 catctcttgc atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt
    12001 catctacagg ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct
    12061 cctgaacgtt ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta
    12121 atagatctag agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc
    12181 cttgatatag tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac
    12241 cttgaatttt gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg
    12301 gacgtggata ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca
    12361 attcatcgat atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg
    12421 accagtcggc aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa
    12481 ttgctttcaa aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg
    12541 aaatatcgac catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc
    12601 gcttttgata tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa
    12661 agccattgga tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca
    12721 taaatattga aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg
    12781 actagagtat atatctaaag aaacaaaatt tcaatacttt tattaaaata attaaatttc
    12841 atttctaaag gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc
    12901 taagcaaaag cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat
    12961 aagaaaccaa ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat
    13021 gaaaataagc attgatcact cttatcaagg cattatggca aactaacaga tagacatttt
    13081 cttcgtcttc gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg
    13141 actccccatt tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt
    13201 tgcttggcag tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca
    13261 gcggcaagct gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg
    13321 aatggatgat tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg
    13381 cctgcatctg tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg
    13441 cgttccaagt tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg
    13501 caaagttccc cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa
    13561 gagaaataat ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca
    13621 tggcaggttt cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat
    13681 aattcaatgg ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt
    13741 ccactttttt ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt
    13801 ccatcccaag ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg
    13861 caaattccgc caacgttcct cgattgtctt cataattttg gcttgcataa ctaatgctca
    13921 gatgttttgg ctggtatttt tctcctataa ccttgccgtt gaatgccccc agaagataat
    13981 ggataatgga tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct
    14041 cgattgtaaa atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta
    14101 cgcttaaaag tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt
    14161 cgatatcttt taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg
    14221 ctttggatcg tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc
    14281 tagattcagt atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag
    14341 accattcggc tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt
    14401 cattttgagc catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat
    14461 tcccttgata aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat
    14521 cgataaatcc ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag
    14581 ccgctacttg ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga
    14641 aatgagtgtc atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata
    14701 gtgtgctgaa ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt
    14761 cgatttccca tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt
    14821 tatcttttgt ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat
    14881 aaggtagttt gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga
    14941 taagtcctag tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc
    15001 cttggctgtc gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc
    15061 tcgtaaaaaa agaccagtcc tattttcaga actggctaaa gaggatgcaa gggttggata
    15121 cacacaaaaa gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct
    15181 tccttgtctc cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa
    15241 tgcacggatc cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt
    15301 tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt
    15361 tttttctaat gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat
    15421 acttaagaca attgggagaa aatcagaaag tccttctagc tcttggtctc tacttccgca
    15481 aatagcgatt attggttttc gatatttttt tgcaagcact gctacaccgt atggaacttt
    15541 tccgtggatc gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc
    15601 gatctttttt tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc
    15661 tcccaacaaa aaaagggctc caccaatacc gcctgctgct ccacttcctg gaatatcatc
    15721 taaatccacc tcccagaaat cttttatttg ctgagcaatc tttcctgctt gtttatctaa
    15781 gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca aattgccaag cagctccttg
    15841 ttgacctgta tatggattgg taacatctgc agcgataatc aattttactt tagaaacaag
    15901 aacgtttctt cgatcaaaat gaagtttttt agtagataat aaaggatttc cttctgtgta
    15961 cccttctaaa gaagcaccca aggattggag taatcctaga ccaccatcgc tacacccgct
    16021 tcctcctaaa gtgacaataa tttgttctac ttcttgaagc agtgcatctt taataagttc
    16081 tcctaaggca taactggaag caagtcggat agtttgatcc gttggtttat caagtagatc
    16141 taaccccaat gcttgtgcag attcgatgac tgcggtgttt tttccttgcc aagttgtcaa
    16201 taaatagctg atttttcgct gacggaataa gagatccatc ccatcaatgg tacgccatgt
    16261 tcccctgagc gcgtaataga ttgcttctgc agtattttct ccgccatctg caatcggcac
    16321 attgattttt cttcctttac gatcttccca gccatttaga actgcctgat ttagttcaaa
    16381 tgaagaagca cttcctttaa atgaatcaat tgccgccaaa atatccatct gtatccctca
    16441 ctttcttctt aagtaagaca ctttttttca tttttttcaa acaaaacaat gctattttct
    16501 tccatttttc tatatactaa aagggaactc attgaataaa ggagagaaaa aacatgaaaa
    16561 tcgcaatagc tggggccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg
    16621 gaaatgaggt cctcttgatc gatcgttggc ctgctcacat tgaagccatt cgtacaaatg
    16681 ggttgattgc tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag
    16741 aagaaatcat cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagccaatc
    16801 aattagatga tatgttttgt tcgatccaat ccattattac tgacaatact tatgttttgt
    16861 gtttactaaa tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata
    16921 tcttatttgg gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt
    16981 taggtgatgg agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa
    17041 aagttgtaga agtctttcaa gaagcaaatt taaatccgat ctatagccat aacgttcgtt
    17101 attccatttg gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg
    17161 attgtaatat tgctgaacta ggcgcgcaaa aagcggcaga gagcatggtt cgaacaatcg
    17221 tatctgaatt tgcttctatc gcagcaaaag aaggaatcat actagatcaa gaagaagtgt
    17281 accaacatat tgcttctact tatgatcctg acaacatcgg gttgcattac ccatctatgt
    17341 accaagacct gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccattt
    17401 ggcgaaaagg acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag
    17461 tccatgcaaa agaaggaatc cttggcgcag aataaaaggc tctctgatga gaatcagctg
    17521 aatcagcagc tggttctctt tttttaagcc caataaaaga tcaaaaagaa aacacaactc
    17581 tcatgatgcc ataagaattg tgcttgtttg tttcagtaca taatctcttc aaaatgtagg
    17641 ttttttattt gttttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat
    17701 cgttagtgat aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgata
    17761 ggattggctt aaatggtgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa
    17821 gcaaaaaaat attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca
    17881 ctagtttctt ttgaaaatac atggattcaa atgtccgcaa agtcactcct tgctgaccct
    17941 cccgcaacag ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag
    18001 taagatatcc aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat
    18061 ctctttttat aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg
    18121 caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa attcatatgt ttcgaataaa
    18181 atgaactatt atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct
    18241 catcgattgc cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt
    18301 gattccaaaa aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct
    18361 ccatatcaaa tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag
    18421 ctgatttaat gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga
    18481 tggaaggcaa aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca
    18541 tcttttccgc ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa
    18601 agccagtaaa acgtttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg
    18661 aaataaatca ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct
    18721 gaaaatttgt tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg
    18781 attgtgaatt taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta
    18841 gctccagaca tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag
    18901 gaagtggcga agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggtg
    18961 ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca
    19021 cgcatcacgg attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta
    19081 ctgattgggt ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg
    19141 cttccttttt gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg
    19201 ctgttttaac tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt
    19261 ggctggcttt tgctaaaatc agtgtcatta tcttactgat catttttgct acttatcttg
    19321 tcggtcagca aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg
    19381 gtgggtttac tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt
    19441 cttacggcgg ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg
    19501 cgattcctaa agcaatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt
    19561 tgtttttgct tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt
    19621 ttgtgatggt atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtga
    19681 ttgtccttgc tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt
    19741 tcttccgttt gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc
    19801 aagtaccgca acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt
    19861 cttattttgt cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat
    19921 tattgatctg gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat
    19981 tatgggataa aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta
    20041 tcctgttcac gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt
    20101 atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga
    20161 aaattttatt ttatttatgt ggaatagatg gagctattga agaaaggagt tgtgacaaaa
    20221 gtcttgttgc aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt
    20281 caaaaaccct ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa
    20341 ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc
    20401 ggtgtttcaa cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat
    20461 atcttcttca gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc
    20521 atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta
    20581 gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc
    20641 aacgacacag cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctggcaagt
    20701 aaacgagctg atttaaaaga tcctgaagta aaaaaattag cggaagaaat cattcaggca
    20761 caagaagaag agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc
    20821 gtccaaataa aaaaagagga tgttccagaa gttatcagct tcaagaaata agaagaagct
    20881 acttctggaa cgccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga
    20941 acagccaaac aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg
    21001 caagcttttg aacaccgttt attaagaggt tgtaaaatca gcgttctgac ctgagtatta
    21061 gagagacaaa tggaaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg
    21121 tcagctgatt gaacaccgtt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgcgtatt
    21181 aagaaaaatt ttgtgaaatg gctttccaca tttttgtata aatttggctt aataccgaga
    21241 aactggcttt tgaataccgt ttattcgtaa aagggattgt gacaaaagtc ttgtcacaat
    21301 ccctttttat gtttttattg ttctaattgc gctgtcttgc ttactgcttc ttgcggtttc
    21361 acaaattttt ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa cacaggagaa
    21421 agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa tgtacttgct
    21481 acgaaacttc cggcaattcc ccaaggcact aagtagttga ttactgttcc accatcttct
    21541 agcgttctac ctaatacagt tggatcaaat cctgcttttt cataagtttc tttaaatgca
    21601 ttaccaggca gaataacaga taggaattgt tcaccaataa aaatatttac tccgatactt
    21661 gtcatcaatg ttgtaaaaat caaatttggt gtgttcgtca tctttcttga tactcgttcc
    21721 atcacggttg agatcaagcc aaattcgatc aacaatccac ctaatgacaa agttagaata
    21781 atcaatgaca cagtaggcat catactttcg attcctccgc ggctaagcag tgaatcgatt
    21841 tgttggtttc cagtatttga aacaaaccct gattcgatga ccccagccaa cttcgttaag
    21901 gaagtttgtg ggttttgaat aaaaatcatt cctactgcta cagcgatatt caacaaaatc
    21961 gttaaaattg ccggcacctt tttccatgca cacagtaaca ttaaaaataa aggaatcagt
    22021 gaccagattg aaatagtaaa gtttgcttct agaacatcag tcacttcacg aatagatgaa
    22081 agacttgcgg ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga aacaacaaat
    22141 gccgggacag ttgaccacat catatgcttg atgtgtttga acaaatcggc atcaacaata
    22201 gctgctgcta gatttgttga ttcggataat ggagacatct tatctccaaa aacggcacct
    22261 gaaacaatag ctccaacaac cagcgctgga tttaagccta gtgttgtccc aataccgaaa
    22321 aatgcaatcc caatcgttga cataacagta aaggcactcc ctacagcgct acctacgatt
    22381 gcacacacaa caaatacaga agggacaaac cactggacac tgatcaattt gaacccaatg
    22441 accattagag ttggaatgat ccctgcttgt atccatacag caatcaatga accgactaaa
    22501 ataaaaataa agattgggat gattcctggt ttgatgccat tgatgattcc ttcgtgcagc
    22561 caatctgtcg ggtatctttt tatcactgca tacaacatga ccattccgat cgctgctaag
    22621 atcgtgatat taggtgagac ctttaatccg acaacgccaa tcgcaatgcc taaaacaata
    22681 accattaata caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc catgataatc
    22741 tctccttttt tagttgttct tatgttctaa aaaagttaag atatcggatt actccgccgc
    22801 aattcatccg cgattcctcc tttataaaaa taaaaagacc tgttttaaac taaggacgaa
    22861 tgactccgcg gtaccacctt aattaaaaac agatctgttt ttcactttca aacttttatt
    22921 caaatgtatg gactttccat gtaattcgtc aattaggctt gatcggttca cagcgaccac
    22981 cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg tacgaagttt
    23041 accttccata agatcatttg tcaatgaaaa agaaaatatt ttttcatttt tctgaaaatt
    23101 atttttataa ctttcacaca caagtttatt ttttactaga aagtagcgcc ctcttgtttt
    23161 tttcttgcta ctatttcttg atgttcttgc ttgattcttt ctaatagctt tggctctttc
    23221 aacagatcaa gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga agcaaggcct
    23281 ttgtcacttt tagcagcagc tttgaattct tctgtgtgtc cggcaatata tgtctcagag
    23341 attgctaccg taggctggat tgttggtatg acttggctga catttcctac atcagaagaa
    23401 ccgagacttg ttctttgttc cgtttctatt tcttcatctg gaacacctgc ttcttttgca
    23461 tgctggaaga ataagtcatc aaaagaagga gtaacgatga catcatcaac tgcattttgg
    23521 aacaatccaa actcaaaagt cgtgcccgta cttatcgcag cacctcgtac gatattttcc
    23581 actttttcat atactttatc taatgtttgc cgatttcctg cacgtaaata gaaacgtcca
    23641 gcagcgtaat ctggaatgac gttggcagct actcctccgt ctgtaatgat cccatggata
    23701 ttgacatctt ttggcaattg caagcgtaga gcgttgattc cattaaacac ttggatcaat
    23761 gcttccaaag catttactcc tttttcaggt gctgctgctg catgggaagc gacaccgtaa
    23821 aatttgatgt cgactggatc attcgctaaa gattctgtcg ttttcccata acgaaaagct
    23881 ggatggacgc ataacgctac atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct
    23941 ttagctgatc cgttctcgcc cccttcttcc cctggggtgc catatacacg gatttctcca
    24001 ccagtttcat caatgaattg cttcactatg ctggcggcta atacggagta agtaccaaac
    24061 aaattatgcc cgcatgcatg accaatacct ggtaaagcat cgaattctgc caaaaaggca
    24121 attgtgggac cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct
    24181 acttcttttt ctacccaaaa cctttctttt tctaattgtt taatcaacgc tgctgaggaa
    24241 taaaactcat agttacttac ttcaggatgt tcgtggatat ctagagcaag tgcttgatat
    24301 tctggtaaac gatattggat tagatccttc aattggtttg ttgagattgt tgttgtcata
    24361 aaaaattcct cctatgctag tcatttttct tattttctcc cgaatgtttc ccatgcaggc
    24421 acgcttgatc cttttgatgt tttctcgatt actttgactg tgtcttctgt ttgataagcg
    24481 tctactattt tttgatacgt tttattgttt tcatcttctt ttcttgcaac gatgatattc
    24541 acataaggac gtgcatggtc atccactggt tctaagaaga tggcatcaga agcaggatta
    24601 aatccagcat ctacagctac accgctattg atcactgaaa tgtctacatc tgacaaagca
    24661 cgtgctgttt gggaagcatc taattcagaa atgtctagat tttttctatt ctcggtaata
    24721 tcagaaacag tgggcgcttg tccttttgcc ttatccactt tgatcaatcc agcagtttgc
    24781 aataacagaa gagctctgcc gccatttgta acatcattag ggattgctac ttttgctccg
    24841 tctttgatat cagctacatc cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca
    24901 gtatttccga tcgatactaa atcagttccg tgttctttat tgtaattgtc taaaaagaat
    24961 tgatgttgga aagaattcaa atcaatcgat ccatctgcta aagccacatt tggttgattg
    25021 taatctgtaa atttcacgta ttccaaatta atgttctcct ttttcaaacg gtctttcacg
    25081 ttgtcccata catctgtatc ttctccaata atccctagtt ttacgatagt ttcttttttg
    25141 tcagttgtat ctgcttttcc ttggctacac cctgccaacc aaataattgc tacaaatgta
    25201 atgactacaa aacgaataat ttttttcatt ttttctcctc cactttcaat gtgtaatttt
    25261 tttaatcagt tgattactga taaattgact taaaaaaact aagattagaa tcaataatgt
    25321 agccacaatc gttacatctg tttggaatcg attataccct ctcgatatag caagattccc
    25381 taatccgcct gcacctactg ctccagccat ggtagtcagt ccgatcacgt tgatgatagt
    25441 caacgccgag acccgaatga taccaggcaa accttctaac aaatacacac gtaaaatgat
    25501 accgattgga ctggttccca tcgcttctgc cgcttcgatc accccaggat caacttctag
    25561 cagtgcgttc tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca atggcactaa
    25621 agctgctttc gcaccaatcg tcgttccaac taagaaacga gtagtcaatg ctaaaagaga
    25681 aagcaagata atgaaaggga tggaacgaat gatattgatc agcttatcaa gtatttcaaa
    25741 tagcagacgg ttagctagta atccattagg gcgtgtcact actaacgtca cacctaagat
    25801 gattccaatc atcccgccaa tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt
    25861 cgcttcgaca aattcatccg gaatcaaata aacattgggc aaatatgttt taaagaaatc
    25921 agtcatttta tcttcctcct tcgatcactt tgatacgact atctaaatcg tttttttgta
    25981 aaatatttat ttttacacca gatttgatca aataatccaa tgcttttttc cgctgtttgg
    26041 tttctccagc taaagtaacg atcaaactcc ctaacggcgt ttcttgtaat aactctacat
    26101 tgccatataa aatattcgct gttacttgat atttgctgta cagatgagcg attagaggtt
    26161 cgttcgtctg atttccaata taacttaatt ccaccagcca tttattggcg atctgatcgg
    26221 caaaagcaga atgggcaatg atcgtttcta atgcttggtc aagatgagta gctgttcgaa
    26281 taaaatcttt ggtcaattct tcttcaggat tgctgaagat ctggatgctg ctaccttttt
    26341 caacgattct tccatcttcc ataaccgcta ccttgttgca gatttctttg acaacttgca
    26401 tctcatgagt gatcaggacg attgttaaac ctaactggcg attcaatttt tttaacaatg
    26461 caaggatctg aagggtcgtt ttcggatcaa gtgcactggt tgcttcatca cataataata
    26521 ccttaggttc attcgccaaa gctcgtgcga ttgctactcg ttgtttttgt ccccccgaca
    26581 gttgtttagg ataagcagat gctttttctt ccaaaccgac aagttccagt agttcattca
    26641 ctttttgacg acgttcctgt ttcgattttc cagaatactt taaagaaaaa tcaacgttgt
    26701 caaaaatagt tcggctttcc attagattga aatgctgaaa gatcataccg atggcctttc
    26761 tttcttttcg caattctttg ggagagagct gactcagatt cgtctgattg acaatgactt
    26821 ctcctgctgt tggtttctgc agcaaattca tcacacgaac taacgtactt ttccctgcac
    26881 ctgagtaacc gacgattcca taaacatcgc ctttttcaat cgttaaatct acagaatcta
    26941 ctgcctgtat ctctttttct ttttgtttga aacatacaga aacatctcgc aattcaatca
    27001 ttaaaattcc tccttatcta aaaaaagcgt cctcaagaaa atgaaaatgt cattttcttg
    27061 aggacgcttt tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct
    27121 tcaagtacga tcatactcta gcaagatatc ggtcgcagcc gtcatcgcct tacatctatg
    27181 ctcgggatga attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta
    27241 ccgaagctct ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc
    27301 aaaaaaaaag tccttttact aacataaact atgtcagtaa aaggacgacg tatcgcggta
    27361 ccaccttttt tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg
    27421 ttcgttttaa cggtcgaaca gttccgaatt tgcctactta tcgacaaatt attctccaag
    27481 gccattttca gcttttctta cttgactctt ctcaccagcc agagcctctc tgcaaagttt
    27541 ccaaacttac tttccttttc aacgaaatcg atattttgtt gttgggatta atctacatga
    27601 ggaaaactga aaagtcaagt acgaaattca aattattttt tctatattta tttttctgtt
    27661 tttttattta acatttcaat aacagttcga agatcttttc cttccgcaag aagtttcaat
    27721 ttttcctttt cttcgacggt ataatttcgg acaaaactgg aatcaggtat caaaacaacg
    27781 cctaatcctg ctcgtgaagc agctgttgct cctactaaag aatcttccac tgcaaaggct
    27841 gaactagctt ctaatcctgt tcgttccaaa gctgtcagat aaggaatcgg tgaaggcttg
    27901 ttctccgaca cttcgtctcc gaatgtaaac gtgtccaagt aatcagctaa tttaaagtga
    27961 tccaagatat ctgttgctct tttcgtaaca gtggtcgtag caatgcctag tttcatatga
    28021 cgttctttta agtctgccaa tatttctttt gcatatggtt ttaagtggat ggctccgctt
    28081 gtcagctgtt gataaaaata ttcttcacgc ttcgctcgga cttcctgtac tttttcaggg
    28141 ctacctgctg ctttcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg
    28201 tcaacgattt cttttgggat atggatatca aattgctgaa atccccaaag ccagccatca
    28261 cgatacgttt tttctgtgtc aactaacaaa ccatctaaat caaatattgc acctttcatc
    28321 tatacattaa tcctttccaa aacaatacac atagtttagc acgactggtc caaaaagaac
    28381 agaagcaaag aaccagcctt ctcgtttata agagttcttg gaatcgacgg atatagattt
    28441 tcttcacagc tgtcacaagc gccagataag cgatgactgt agcaaataac cataaccagt
    28501 aattgccagg aagtggcatc aagcctaatt cagcaccaaa acttgtaaaa ggaaggacag
    28561 aaccgacaat aattcctaat gtagtgataa caaacatggc aaatgttgcg ttgctttgga
    28621 taaatggtac tttcggtgta cgcagtgcat ggagcactaa ggtttgtgtc catagtgatt
    28681 cgacaaacca tcctgcatgg aataatgcga tgaaaatcac tcgctgtgaa ctatccaatg
    28741 tatggaaact tccgcccact acagcaggac aaataacaaa atacatcaac aagtaagtag
    28801 taatatcaaa tatcgaactt gttggaccaa agtaaaccat gaattcccca atactagatg
    28861 cattccattt ttttggctcg tccaaatatt ctttatccat ccgatcccat ggcaaggaaa
    28921 tgcaggaaac atcatagatc agattcaaaa acaaaagttg taaaggcagc attggtaaaa
    28981 atggcaggaa cgtacttgca atcagcacgg aaaacatatt gccaaaatta gaacttgctg
    29041 tagccttgat atatttcatg atattcccaa aagtttctcg tccagaaaga aggcctcttt
    29101 ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc agattctttc gcaatatcca
    29161 cagctgtatc tacagaaata ccgatatcag ccactttcat tgcaggagcg tcattgatac
    29221 catctcctaa aaatccgaca gtatgaccat ttttacgtaa agctgtcgtc aaacgtgctt
    29281 tttgctgagg attcaatttt acgaatactt catggtcttc agctacttgt gctaattcat
    29341 gatcactcat ttccgagatt ttctcaccgg taaccaattc attgatctct agacctactt
    29401 gacggcacac tgaacgtgta actaattcgt tgtccccagt cagaactttt acagctacac
    29461 catgttcttt cagagctttc aacgcttgtt tcgttgtttc ttttggtgga tcaaggaaag
    29521 caagataacc aatcaaaacc atgtctgact catctttgac tgaaaattct ccaacgacgc
    29581 ttggattggt cttttgtgcg acaccaatca ctcgtaagcc atcttcatta agtgactgta
    29641 cttttgcaag tacggcttct tttcgttctt ttgttaatgg tgtaacagta ccgccaagat
    29701 caataaattt agaaatgttc agcatttctt caatcgcgcc ttttgtgatc atttgtgttt
    29761 taccagctgg atcagcaacc acaacactca tacgacgacg ttcaaaatca aaaggaattt
    29821 catccacttt ttgataattg atcttatctg tatctaaagt ttctgttgct tcatcaatga
    29881 tagccacatc taacaaattt ttcagccctg tttgatagta gctatttaga tacgcatggc
    29941 gcaaaacacg atcatcttca tttccttcac aatccatatg atattcaaga atgattttat
    30001 cttgtgtcaa tgtccccgtt ttatccgtac ataacacatc gattgcacca aagttctgga
    30061 tggcattcaa atttttgatc actgttcctt ttttggccat cacacttgct cctttgacca
    30121 aattcgtcgt aacgatcatt ggaagcattt caggtgttag accaactgct acagatagtc
    30181 caaaaagaaa agcttctagc caatcccctt ttgtaagccc attgatgacg ataactgctg
    30241 gagccatcaa tgacataaat ttgatcagta gcatggatgt tttatggatg cctaattcaa
    30301 agcttgattg aattggtttt tcagatagcg tttctgctac atgaccaaat agtgtttcat
    30361 ttccaacaga aacaatgatt ccttccgcac ttccactgat gacattactt cccataaaga
    30421 caagattttc gtaacttgtt tcgctttgct gatcgtcttc acatactttc gcatgctttt
    30481 ctactggata gctttcccct gtcaatgctg cttgggaaat gaataaatcc tttgcggaca
    30541 ataaacgcat atccgctggt atcatatccc ctgctgaaag ccgaacaaga tctccacaga
    30601 cgatttcttc cataggaatc tctgtgtact ttccattacg ttttacagct gccgttactt
    30661 tcacaagtga tttcagtttt tcagccgctt gattggaacg tacggactga atcaacgtca
    30721 ttgtcccgct gacgataacc atagtgaaga taatggctac accgaaaaga tctttatctc
    30781 caggagctgc taatacatat tccgtgatga atgaaataat tcctaatgcg atcaacacta
    30841 atgtaaatgg cgtgatgtat gctttcaata tttctacaat gaatggtgtt tttttgccat
    30901 atgaaatcgc attttcaccg taattttctc ttgaacgttc gatttgttct tctgtcaatc
    30961 catttgctga tgcatggaat agttcaaata attcttgttt cgtttttcca gcaaaagctg
    31021 catagatttt ttgtgtatct ttcttttttt ccatctcaat ctctcctttg tgtccaaaag
    31081 acagctgttc tcgctgcttt tggtctttag ctattcatcg gatcgataga gaaaaaactg
    31141 aaaggggctg agaacagata gataatagcc ctggcctttc ctctagatcc ttgtttttcg
    31201 aatgactgtc atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc
    31261 aatcattttt cgaatagtta tttttccaat tagaccacct ccattaaaaa aagcttctca
    31321 gatctcatcg ttgagttttg acactgtaca acgtaaaaag cagatacctg cttttagcta
    31381 ccttatgaaa agccttatct cgacaattcc tgttctaccc attggcgtct ctcgacgttt
    31441 ttgggcagta gcctatgttt gtataggagc ctcacctaac aaatcttatt cggttttcct
    31501 tcgttaatat aacaactaaa aaagaggatg tcaaatcaat tttccatgtt tttcatactt
    31561 tttttaaaat acacacgtta tattcctgtc tcacgcggtt ttatttgtct cattttccac
    31621 ctcccgctat aatagaaata tagtaggaaa cgaggtgctg ttatggaaag aacacttgtt
    31681 attatcaagc cagacggcgt gagacgccat ttagtcggaa gcatcatcca acgttttgaa
    31741 gcaaagggac tggccattgc tgaaatgaag tttgaaacta tgactcctga attagcaaaa
    31801 gaacattatc agcacttgac tgagcgcagt ttttttgatg aactgatcga ttacatgact
    31861 tccggtcctg tcgtttatct tgtgctggtc ggcgaggaag tgatcgatat cgtccgtaag
    31921 atggttggag caacaaaagc tgccgatgct gttccaggta cgatccgagg agactacgcg
    31981 ctgccgggga ctgaaaacat cattcacgct tctgattccc gtgatgctgc ggtaaaagag
    32041 atcgcccgct ttttcccgcc attcgatata aacaaaccgt ctgaaaaagc ggcgaagtga
    32101 tacaaattct aaactgctgg tctagttgct gggttcagca atggccagca gttttttctc
    32161 taaggacctc cgttgattct ttcctaacat atattcgagc actgtgaaga attcttggta
    32221 gtctttcgtc ttttcttccc ctaaagcagc agaaatcaat ctttgataat aatctgccat
    32281 caatgtttca tagatcactg tacttctttc tttatgtgct aagctccgtt tcatatcttc
    32341 attcacacta cgtgcctttt tcaaccgacc attttctata taaaacgaag tcaggttcat
    32401 taaaaaagta aagagatact gctcatgatt gttcaactgt gctaatcgtc gcattctttt
    32461 tttgattttc aaataattgt tctcaatgaa ccgatcactg aatatccaag gaaaatcagc
    32521 tagaatagat agttcaaatt gaccatagtc tggtatctgc atcaagtatt cttcaatgat
    32581 tcggcattca taacgtaaaa attcaaattc tgtctcagat acttctgttt tttcgaccat
    32641 gtgaagattc aaaaccaaat aatacagatc ttctgttttc ttaaaatgcc tacggttatt
    32701 gctgatctct ttataaggat caaagctgtc ttcttgtacc cgatctagca ttcgtctttt
    32761 tttctttaac tttattggtt catccagctg gtttcgcatc cattcatatt ctgcaagtgt
    32821 catgttcatt cgctctaaaa atgatctcaa tgttacaaat gacatttttt tcactgaatt
    32881 tgtctcaatc ccgactagag aagtcttaaa acagattcct tccgctaatt gggactgtgt
    32941 caacttcttg ttgactcgaa gtttcttgat caattcacca tcatacataa tagttctcct
    33001 ttttaattta aatttttcca cattcaataa atagatacgt taaaaaggaa aaatacacga
    33061 aaaaaataaa aaaatgtttt tttcataaaa aataccaacc ttcaagcatt agaatgttgt
    33121 ctaactactg aaggttggtt tgattgttta ttgattattt ttttgctgtt caacgtctgt
    33181 tcttgttact ggatataagt tggttgttgt tttattacca ttttttgaaa agacacttgt
    33241 cgaatgttcg cctttttgct tttcgattgc ttcattacgt tccgcttcat ttttatagtc
    33301 atattttttc ggatcaatcg tctcaaatcc gtttggcaca taaaaccgca agagattttc
    33361 ctgattcagc ttatctgaaa gacttaacgc agtcttgact ttttcctgat ctttggagat
    33421 ttcctcatcg attcctgctg ctttcgtatc gatgatttct cctgtctgat tattgtaagc
    33481 ttttcctcct aggacggtgt atttgggtgt cacaaagttc ccatttcgaa aagcaactac
    33541 ttgatcatgc tgagaagata ataagtctgt accaaaatga agataatctt tatcatcgat
    33601 tcccaataga tgtagtaatg tcggcaaaac atcgatttct ccaccgtatt catgattgac
    33661 tgtcccttta gtataaccag gcatatggat catcaatggc actcgttgca tctgtgcgtt
    33721 gtcaaattca tcccatgtat ctggatcttt accaagagcg cttgctaaat ctttattatc
    33781 tgtattcgag atcccaaaat gatccccgta aatcacaaag attgaatttt ggtaaatacc
    33841 agattttttc aaatacgtaa aaaattgttc caatgattga tccagataat gtgccgttcg
    33901 aacataatta tctactgtgc tattgccagt attcaaagat ggaaagtcga aattcttgtc
    33961 atccgtataa taaggcgtgt ggttggttac ggaaagaaat ttcgtgtaaa acggctgttg
    34021 tagatgctca agatattggg cagattctct gaataaatct ttatctaata tcccataacc
    34081 taacgtttcg tcggattcat caaaataaga tcgatcaaag aaattgtcat aacctaagtt
    34141 tttatataca tgatctctat tccaaaaact agctacattc ccatggaaaa cagcgcttgt
    34201 atagccttgc ttttgtccaa ggatcgctgg tgcggcttga aagacattgt ctgacccaag
    34261 ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca gtttccaaca tattttcagc
    34321 atcacttgtc tttccctgtc ctacttcatg aaagaaattg tcaaagctga tcgttgcttg
    34381 attctggaaa atactattta aaaacggtgt gacttcttgt ccgtctacct tcatattaat
    34441 caaaaactgc tggaaacttt ctaaatggag aacgatcaca tttttccctt tagcgatccc
    34501 aaatgtttcg ggatttggct ctgcataatg tttctttgta taatctaaaa cttcatcaat
    34561 cccattactg cttgcatgcg cgcgaacact gctagtcatt cctgttttga ttccgtcata
    34621 aatagtatac gcatcgattc ctagatattt gacaatatat gaacggtcaa atgttcgctg
    34681 cagcaattgt ggacggttgg cttcgctcag cgctagattt cctgaaaaaa taagccctga
    34741 aagacaggta acagcgatag ccatcggttt gccgacaggg ttgcttttga taggtatttt
    34801 cttgaccttc aaccaaacca gtagcccaat gaacacagcg atatccagcc agtagatgac
    34861 atcatggggc ttcatcaaag aaaaggagct tcctgacaat ccttgcgaca ctttcgaaaa
    34921 gcctaataca gatttgactg taatgaaatc tgtgaattcc cgataaaaaa taatattcaa
    34981 gtacaacaaa agcgtattta aaatatcaat gatcaaaaga atagctaacg ctggctttgg
    35041 ctttttaata tacaaagaaa ggccaaaaaa caataaggta gtcgcaattg ggttgatcca
    35101 caagataaag tactggatcg taccttccac acctaatgaa aaatctgcat agtaggcaat
    35161 gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg attgctaaac gattttggca
    35221 caatttttta agttgtgtca cgtatatcac ttctctctat tcacattttt tcttattttg
    35281 ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt aagatttttt tattctttct
    35341 aaagattatt ccgtccactt ttcacagcat aaaaccgctg cgatgtaagc gattaaacta
    35401 atgaagagat aaaagtcttg tggtgaccgc ttcactccac ggtattcaaa agccagtttc
    35461 tcggtattcg tacaaaaaaa gaggctgtga caaaattctt gtcgcagctc cttatttctt
    35521 gatgcaggac agctttttca caacttcttc cttaaacggt gttctcctgc caactcctcg
    35581 gccacaagtc acaatattta taaaacatga gaagctgttt tttgaaattg ttccttgtgg
    35641 ctcagagtta aacggcaggc tcaagacctc ttgatacacg gtgttcaatc agctgaccct
    35701 cggtattaga ttatgtcagg acgctaattt tacaacctct tggtaaacgg tgttcaaaag
    35761 cttgcatctg cggtaataag cccaaccaaa cgaaaaatat aaggagctat tttttgaaat
    35821 tgctccttgt ggctcagagt taaacggcag gctcaagacc tcttgata
//
LOCUS       DACDYM010000024        34660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDYM010000024 DACDYM010000000
VERSION     DACDYM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.024"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(313..2205)
                     /locus_tag="IWM59_001154"
     CDS             complement(313..2205)
                     /locus_tag="IWM59_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979201.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="IWM59_001155"
     CDS             complement(2202..3929)
                     /locus_tag="IWM59_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979202.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="IWM59_001156"
     CDS             complement(3945..4595)
                     /locus_tag="IWM59_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9979203.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="IWM59_001157"
     CDS             4799..5095
                     /locus_tag="IWM59_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9979204.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="IWM59_001158"
     CDS             5287..5907
                     /locus_tag="IWM59_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9979205.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="IWM59_001159"
     CDS             complement(5999..7165)
                     /locus_tag="IWM59_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9979206.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="IWM59_001160"
     CDS             complement(7177..7530)
                     /locus_tag="IWM59_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9979207.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="IWM59_001161"
     CDS             7658..8680
                     /locus_tag="IWM59_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9979208.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9119..9553)
                     /locus_tag="IWM59_001162"
     CDS             complement(9119..9553)
                     /locus_tag="IWM59_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979209.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="IWM59_001163"
     CDS             complement(9663..11522)
                     /locus_tag="IWM59_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9979210.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12799)
                     /locus_tag="IWM59_001164"
                     /pseudo
     CDS             complement(11640..12799)
                     /locus_tag="IWM59_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13106..14530)
                     /gene="araA"
                     /locus_tag="IWM59_001165"
     CDS             complement(13106..14530)
                     /gene="araA"
                     /locus_tag="IWM59_001165"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9979211.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14546..15244)
                     /locus_tag="IWM59_001166"
     CDS             complement(14546..15244)
                     /locus_tag="IWM59_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9979212.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15201..16862)
                     /locus_tag="IWM59_001167"
     CDS             complement(15201..16862)
                     /locus_tag="IWM59_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9979213.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17080..18480
                     /locus_tag="IWM59_001168"
     CDS             17080..18480
                     /locus_tag="IWM59_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9979214.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18732..19811)
                     /locus_tag="IWM59_001169"
     CDS             complement(18732..19811)
                     /locus_tag="IWM59_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9979215.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19897..20856)
                     /locus_tag="IWM59_001170"
     CDS             complement(19897..20856)
                     /locus_tag="IWM59_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9979216.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20868..22391)
                     /locus_tag="IWM59_001171"
     CDS             complement(20868..22391)
                     /locus_tag="IWM59_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9979217.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22401..22964)
                     /locus_tag="IWM59_001172"
     CDS             complement(22401..22964)
                     /locus_tag="IWM59_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9979218.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22970..23833)
                     /locus_tag="IWM59_001173"
     CDS             complement(22970..23833)
                     /locus_tag="IWM59_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9979219.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23847..24743)
                     /locus_tag="IWM59_001174"
     CDS             complement(23847..24743)
                     /locus_tag="IWM59_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9979220.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24918..26189)
                     /locus_tag="IWM59_001175"
     CDS             complement(24918..26189)
                     /locus_tag="IWM59_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9979221.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26179..27696)
                     /locus_tag="IWM59_001176"
     CDS             complement(26179..27696)
                     /locus_tag="IWM59_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9979222.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27894..28784
                     /locus_tag="IWM59_001177"
     CDS             27894..28784
                     /locus_tag="IWM59_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9979223.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="IWM59_001178"
     CDS             complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="IWM59_001178"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9979224.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29693..31165)
                     /locus_tag="IWM59_001179"
     CDS             complement(29693..31165)
                     /locus_tag="IWM59_001179"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9979225.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31802..32164)
                     /locus_tag="IWM59_001180"
     CDS             complement(31802..32164)
                     /locus_tag="IWM59_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9979226.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32387..33139
                     /locus_tag="IWM59_001181"
     CDS             32387..33139
                     /locus_tag="IWM59_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9979227.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33279..34202)
                     /locus_tag="IWM59_001182"
     CDS             complement(33279..34202)
                     /locus_tag="IWM59_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9979228.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34217..34489)
                     /locus_tag="IWM59_001183"
     CDS             complement(34217..34489)
                     /locus_tag="IWM59_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979229.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10229 a         7212 c         5980 g        11239 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg
    12061 gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt
    12121 tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc attttttcta
    12181 tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt
    12241 aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat
    12301 cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg
    12361 tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat
    12421 aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca
    12481 tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt
    12541 ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt
    12601 tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg
    12661 taagccattc atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat
    12721 ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa
    12781 tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca
    12841 attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta
    12901 ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc
    12961 aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa
    13021 gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac
    13081 taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt
    13141 ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc
    13201 cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca
    13261 cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga
    13321 tcagcagttt ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt
    13381 tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa
    13441 cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga
    13501 ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc
    13561 ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag
    13621 caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc
    13681 catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc
    13741 gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat
    13801 aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt
    13861 cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc
    13921 aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt
    13981 tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact gcaacatcca
    14041 tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat
    14101 tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat
    14161 ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag
    14221 ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg
    14281 agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca
    14341 tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca
    14401 attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca
    14461 gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa
    14521 tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt
    14581 gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga
    14641 gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg
    14701 cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa
    14761 ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat
    14821 attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat
    14881 aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga
    14941 tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata
    15001 aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt
    15061 ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt
    15121 tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga
    15181 cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca
    15241 gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc
    15301 ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg
    15361 atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc
    15421 ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg
    15481 gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact
    15541 tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat
    15601 ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg
    15661 aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt
    15721 actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta
    15781 tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata
    15841 cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag
    15901 ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc
    15961 ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc
    16021 gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc
    16081 ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa
    16141 gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg
    16201 tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt
    16261 tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg
    16321 cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa
    16381 ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc
    16441 catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg
    16501 attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga
    16561 taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag
    16621 atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct
    16681 tctaatgaat aggtccagat accattttct agtttgtttt cccactcata actaccagcg
    16741 gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca
    16801 atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc
    16861 ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct
    16921 tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa
    16981 taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga
    17041 tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt
    17101 tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt
    17161 ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct
    17221 tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta
    17281 tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt
    17341 tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt
    17401 gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat
    17461 atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg
    17521 attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa
    17581 acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc
    17641 ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag
    17701 gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa
    17761 atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta
    17821 gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa
    17881 ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca
    17941 gcgagcgatg cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg
    18001 ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc
    18061 gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca
    18121 aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg
    18181 gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca
    18241 tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct
    18301 gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt
    18361 ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa
    18421 atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa
    18481 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt
    18541 cacaaggaac aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc
    18601 agttggtagg agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg
    18661 atgacaaaag ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa
    18721 aaacccgtct cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca
    18781 tagagaatat ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca
    18841 tcttgtccca acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg
    18901 cttaaagaag aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt
    18961 tctgtcaact tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct
    19021 aaagcttgtt cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc
    19081 gtggtgaaag taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg
    19141 ataaaacctt tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga
    19201 cgctgatgtc ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct
    19261 aaacaaatat aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg
    19321 ccttgttctc tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta
    19381 ggctcaacga tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc
    19441 aattcgtgat cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca
    19501 ttttcgattc ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca
    19561 ccgatcgttt ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat
    19621 gtgcccgacc ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc
    19681 cgaatcgtat gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt
    19741 gtatttactt cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga
    19801 tattttgcca tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc
    19861 aaggtttatt caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc
    19921 accaaattct ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt
    19981 aggattgtct aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaagt
    20041 atcaaagcac ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt
    20101 tttttcagaa gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag
    20161 attcttgtgc tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc
    20221 agaatacgta atgaatattt tcccattgcg agtaaggact gctggacctt catttactga
    20281 aaaaccccgt ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa
    20341 cgtccaagga ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc
    20401 ttgttgggcc cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact
    20461 gaaagtttcc cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc
    20521 tatatgctga ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt
    20581 atgattgctt gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc
    20641 ccagatcaat tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc
    20701 atgttctaat tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac
    20761 cgatcctgta aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg
    20821 ccgaacgata ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt
    20881 attctaatta cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct
    20941 attgaaaaat agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc
    21001 aacagatcgc tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt
    21061 tcaatcgtta tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg
    21121 ccgttatcta cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa
    21181 acagggcaaa tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc
    21241 atcacataat aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt
    21301 attgtgttta ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata
    21361 gccagcccta atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg
    21421 ggactaccga cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca
    21481 tcaaacgcta gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta
    21541 gcaacgattt cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta
    21601 tcatcacgct catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag
    21661 ttatgcaaag ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat
    21721 tcagggtacg atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca
    21781 tccactaact tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc
    21841 ttagccccca cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca
    21901 aaaggctttt tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa
    21961 ttacaatatt ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg
    22021 aatatcggtt tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa
    22081 tttggttcta tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct
    22141 acagtatcca cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct
    22201 aattctaatt cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt
    22261 tcatggcccg gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag
    22321 ccaaacagcc gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact
    22381 tcttcgtcca acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt
    22441 ttccatacgc caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca
    22501 aatcctaata acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat
    22561 gcactttgtt caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc
    22621 caggataaaa gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg
    22681 attgtctgtt cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta
    22741 aggaacaagt tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt
    22801 cctgatccct cacctttcca ttgcgccatt acataaaaaa cagcacatgt agcaggcacg
    22861 atagtaataa caggcaaaca cacgattacc cagagaatac ctaataagat gtaatcagaa
    22921 accacaatca actgtccata aagtttagtc tcaactattt tcatcttcct cactctttca
    22981 ttccagacat agctgttcct tgaacaaaat atttctgtgc aaaaatataa accactagta
    23041 atggcagcat tgaaataacc gttccagcca ttacaggtcc ataaaaagaa atgttctgcc
    23101 ctgtaaataa agctaaccct gctggtaaag ttctcatttt tgaactactt gtcaatatca
    23161 atggataaag caagtcattc caactattca tcagcgtgaa aatccctaat gataaaagag
    23221 taggtttgct caatggcaac atgattttca aaaaaatatt aaactcactt aatccatcaa
    23281 tgcgtgctgc ctcttccaaa gaatctggta aagtaataaa aaaggaacgc atcatgaaga
    23341 taccaaatgc agttgtcata cgaggaatga tcaaacctgc ataagtatca agcatgccaa
    23401 gcaaattgac ttcaataaat agaggaatca tgaagacttg gaatgggatc atcattgtca
    23461 gcaaaacgaa atagaacaaa actgattttc ctttgaaacg catacgagca aatgcatacc
    23521 ccgcaagaga atcaaagaat acagaagtga tcactgtccc tccagcaaaa atcactgtat
    23581 ttttgatgta atctaataaa gggatcgttg tccaaacctt aatatagtta tctagggtat
    23641 agctatctgc aaatattcta ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag
    23701 aaataatcag ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc atactaatcg
    23761 ttcgaatgat ttttttacca ctaactgctt ttcgactatt cacagtaact gtaatcgagc
    23821 tttctggtgc catacaggtt tccctcctat ttcatttgtt tttctttccg tgacatgtac
    23881 acattagtgg agatagctat agttgcaaca atgataaaca aaaccgaaga cagtgctgat
    23941 gcatagccca attcataagg tgctgtaaat cctcgactgt aaatatattg aacagctgtt
    24001 tctgtcttaa attgtgggcc accagcggtt gcaacatata cttggtcaaa cacttgcatc
    24061 gaaccaatca gattcgttaa aatacaaaaa cctaaggagg gaacgatctg aggtagcgta
    24121 atgttaaaaa actgctgtcg atttgttgca ccatccattt gagcagattc atacaaagag
    24181 ggagagattc cctgaatagc agtcaagaga atcgtcatag taacaccaaa attcttccaa
    24241 acagtcatga tggctaccgt cggcatagca agggttggat cgcgaaaaaa ttgagggatc
    24301 ggcaagccga cttgtgaaaa caagtaagga atcgccccta cattcggatc aagcaacata
    24361 gaaaaaataa tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga
    24421 caaaaacgat tgaatctggt atttttagat aatgctactg ctgctaataa accaacgata
    24481 atttgtgttg gcgtttcgag tacggtgaaa tataaagaat gaatcaacga attaactgct
    24541 cgttcatcac taaatatacg tgtgaaattt tcaaaactta caaaatttct tgatgtaaag
    24601 aaaatattca aatctgtaaa actaatagca aatgttccta aaagcggtat gacagtaaat
    24661 aataataaaa ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc aggacgattc
    24721 caatatttgg tgaatccgtt catatagtcc acctcatctt tcattttgat aaaatataca
    24781 atcttggttt aaacgaatct gaataagata tccgaacaat cgctgtgatt tttgcaataa
    24841 cacctatctg caaatactag gtcgatcatt cagatatctg tatcttgcca acagtctatc
    24901 ctgtataaag ttctgtacta tttttgcagt aattgattga tggcatcatc tgcatcgttc
    24961 atgagtgtct caggatcttc tcctgctaat aatttttcaa tcagtggatt gatgatatcg
    25021 ttattgatcg cagagatttg ggtaaaacct ggtaaatatg gctctgcata gtccatttgt
    25081 ttcgataatt cagatacaat aggatcattt tttacttctt catcttttgc tactgaatcc
    25141 aagtaggcag gaaaaccatt ttcgatactc cattttttac caatttcagt tgtattccaa
    25201 tatttcaaaa actcatagac agcttctttt ttattattat ctgtacttgc cggaatggca
    25261 tatcctacgc catctaaaat agctgttttt ttgtctgaat cactcccttg tggtaaggta
    25321 gtcacattat aatcaatctc gtttgctttc aatccgttat ttaaccatgg cccattgatt
    25381 tcaatggcta gttgaccagc attcattaaa ttgtccattt cggcgccaga aatcgattct
    25441 ggaccagtct tgtctacatg gatcattttt tggatcgctt ttaacgtttt aaggttctca
    25501 ggagacgcaa attcagactt agtataatca tcgttcacta atttaccgcc atttgctaac
    25561 atccagttat ataacggtgc agttccatcc ttattgaaga caaaacctga tacattttta
    25621 ctactatcag ttaattttgg tgccatttct gtcaattctt cccaagtagt aggtggttgt
    25681 tcagtatcta atccagcttg tttaaacaaa tcattattcc aatacatata catgccttgt
    25741 acctgcatgg ggacaaaata ctgtttacct tctacttcac ctaattcaat agccgtttct
    25801 ttaaaatcag atttatctac tccatcatat tcccagaaat cgtctaatgg ttgtaccgct
    25861 ccgttttcta catactgtgc aaaatctgga taatttaatg caataaagtc aggtgctttt
    25921 ttagaagaaa tcgctgtagg aagtttttca ttcaaattgg cccaagtcat gatatccatt
    25981 tcaattttga tgtttttatc atttgtttta ttaaattcat taacgatttc ttgcaatatt
    26041 tcaccatcag atgcagtaaa cccattccaa aaagtcaaag tcacttcttt atcagaatca
    26101 gattctgctg aacttccgcc accgcaagct cctaaaatac ctgttgcagc taccaacaaa
    26161 gagccaatta accatttttt acctttcatt ttttgttcct ccttcatctg ataaacgaca
    26221 tctgaccaca ttccaagata gcggtttcaa taaacaattg attttcccat cttcagcttt
    26281 tacatttgtt gaatgacttg gttttacttg ttcactttta ggaagattag ttgcttttgt
    26341 atcaaatcca ctcatttcag aaaaatcaat cactccctct aatgataatt cagacaagtg
    26401 aacagttaga ttcatttctt cttctccacg gttaactgca aatatgacaa gttctttgtc
    26461 atttattacg gctatacttt ctaaatacgg cacttcttca aaatcttccg tcgcatagtt
    26521 tgctgaacga atttgtgggc ttaacacctc tccttgtcca tacacagcag cttgcataaa
    26581 tggataatag atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat
    26641 aacatttacc aattgagcaa gacaagcaat ctttactcta tctgcatgtt tcaataaagt
    26701 gattaataga cagcctaata gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg
    26761 aggagcaatc tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat accaaatatt
    26821 ccattcatca aaggacaggt tgatctgctt cttactatgt ttcttcgcct tgactgtatc
    26881 gcacatcgct gtcacttcag aaatgaaccg atccatgtct aaggaacgtg caagataatg
    26941 agaaagatca tttttttggt tgccataata ttgatgcaga gacaaataat ctatttgatc
    27001 gtacgcttga tcaagaactg ttaactccca atcaccaaat gtaggcattt gccgattcga
    27061 acttccgcat aaaaccacct caattgaatc atctacgagt ttcatcgcct tagccgtttc
    27121 ctctgctaac cgtccatatt cttccgctgt tttatgtcca atctgccaag gaccgtccat
    27181 ttcattccct aaacaccatg tcttaattcc aaaagggctc tcttgaccat tcgaacgcct
    27241 taaatcactc caataagttc ctttaggaaa attacaatat tcgactaaat tacgagctgc
    27301 atctactcca cgagtaccca gattgacagc catattgatt tcggccccca catgctgaca
    27361 ccagttataa aattcatgaa tcccaacttc attcgtttca attgtccgcc aagctaaatc
    27421 caaccttcta ggtctatttg ctatgggccc aatactgtct tcccaacgaa atcctgacac
    27481 aaaatttcca ccaggatatc taatcatggg aatgttcaat tcttttacca agtgaatgac
    27541 gtcttggcga aatccacttt tattagctga tggatgatct ggctgataaa tcccttcata
    27601 tactgctcgt cccatatgtt caataaatga actgtacaac cgcggatcaa ctttgcctac
    27661 acgcttgaca ctactaatca acattgtggc ttccataact gacctccttt aatttataca
    27721 tgaataatac acctcgttga aagcgttgtc aataatataa atgttaaact tacttatttt
    27781 ttatcgattt taattaacca aaatattaat aatgattaaa acaaataatt taatataact
    27841 tttattaaca aatatgttaa tatataagaa aaacctttta agaggtgata tatatgcaac
    27901 tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa acaaggatca
    27961 agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg
    28021 gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca aacatcatca
    28081 aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa atcgatatca
    28141 gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata cctattggtc
    28201 actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat gattttatcg
    28261 ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc cgcattctct
    28321 ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca gaggatacat
    28381 tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca aatgacaatt
    28441 ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg acgagccctg
    28501 gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat aatcttaatc
    28561 aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg aatggagaac
    28621 ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg catttgcgaa
    28681 tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt aaaggattcg
    28741 gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt tcttttctat
    28801 tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct ttattgttat
    28861 ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac
    28921 catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag ataatcatca
    28981 atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt cccatcttca
    29041 agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc ttgaagcaat
    29101 tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc accatcacca
    29161 aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa tacggctcct
    29221 gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt gatatttcct
    29281 ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg tgcatcaaca
    29341 gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag agcagctttt
    29401 gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa ttgatagttt
    29461 tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg gcctgctaaa
    29521 gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg atattttttc
    29581 aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc aattgttggt
    29641 ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc tcctatgctt
    29701 ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt ttatggttcc
    29761 agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt ttatactctt
    29821 cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt gcaggtaatt
    29881 cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct gtaaagtctc
    29941 gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct tcttcttctc
    30001 gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca aaatttcgag
    30061 tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg cttgataact
    30121 tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg aatactgcac
    30181 caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat acatcaattt
    30241 tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc gaagcataga
    30301 tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta gatatatagg
    30361 ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct aattctttcg
    30421 ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg actaagaaca
    30481 cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt ccataagatt
    30541 ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc ccagcacgta
    30601 cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag actgctgcat
    30661 ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg atgacagatt
    30721 tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc aacagtgctg
    30781 tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt tcatttgcaa
    30841 aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat
    30901 actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata tcggaatcat
    30961 tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa accgcgtatc
    31021 cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac
    31081 ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc agcgtcaaac
    31141 tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt ttcatttcct
    31201 acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc tgaaaaagca
    31261 catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact atcctttctt
    31321 attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga
    31381 agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat
    31441 cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta caacataatc
    31501 aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag
    31561 aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat
    31621 accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta
    31681 agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt
    31741 gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt
    31801 attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt
    31861 tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt
    31921 ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt
    31981 tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg
    32041 ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca
    32101 ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct
    32161 tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat
    32221 ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt
    32281 ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat
    32341 ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat
    32401 aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat
    32461 tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga
    32521 agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat
    32581 ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca
    32641 aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact
    32701 tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca
    32761 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
    32821 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
    32881 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
    32941 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg
    33001 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
    33061 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
    33121 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
    33181 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
    33241 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
    33301 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
    33361 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
    33421 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
    33481 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
    33541 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
    33601 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
    33661 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
    33721 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
    33781 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
    33841 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
    33901 ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
    33961 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
    34021 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
    34081 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
    34141 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
    34201 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
    34261 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
    34321 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
    34381 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
    34441 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
    34501 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
    34561 aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa attgaataca
    34621 ttataatgat actgtaaata tatctttatt attagtgaat
//
LOCUS       DACDYM010000025        34636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDYM010000025 DACDYM010000000
VERSION     DACDYM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.025"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWM59_001184"
     CDS             591..1280
                     /locus_tag="IWM59_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9979230.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWM59_001185"
     CDS             1267..2475
                     /locus_tag="IWM59_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9979231.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWM59_001186"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWM59_001186"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9979232.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWM59_001187"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWM59_001187"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9979233.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWM59_001188"
     CDS             complement(6578..7273)
                     /locus_tag="IWM59_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9979234.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWM59_001189"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWM59_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9979235.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWM59_001190"
     CDS             complement(8472..8663)
                     /locus_tag="IWM59_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9979236.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWM59_001191"
     CDS             complement(8684..9574)
                     /locus_tag="IWM59_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9979237.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWM59_001192"
     CDS             complement(9561..10328)
                     /locus_tag="IWM59_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9979238.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWM59_001193"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWM59_001193"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9979239.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWM59_001194"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWM59_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9979240.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWM59_001195"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWM59_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9979241.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWM59_001196"
     CDS             14944..15282
                     /locus_tag="IWM59_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979242.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWM59_001197"
     CDS             complement(15450..16208)
                     /locus_tag="IWM59_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9979243.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWM59_001198"
     CDS             complement(16226..17044)
                     /locus_tag="IWM59_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9979244.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWM59_001199"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWM59_001199"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9979245.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWM59_001200"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWM59_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9979246.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWM59_001201"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWM59_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9979247.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWM59_001202"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWM59_001202"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9979248.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWM59_001203"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWM59_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9979249.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWM59_001204"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWM59_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9979250.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWM59_001205"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWM59_001205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9979251.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWM59_001206"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWM59_001206"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9979252.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IWM59_001207"
     CDS             27086..27385
                     /locus_tag="IWM59_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9979253.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IWM59_001208"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IWM59_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9979254.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IWM59_001209"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IWM59_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9979255.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IWM59_001210"
     CDS             28352..30325
                     /locus_tag="IWM59_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9979256.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IWM59_001211"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IWM59_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9979257.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IWM59_001212"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IWM59_001212"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9979258.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IWM59_001213"
     CDS             33204..34496
                     /locus_tag="IWM59_001213"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9979259.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DACDYM010000026        33817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDYM010000026 DACDYM010000000
VERSION     DACDYM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.026"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            299..1960
                     /locus_tag="IWM59_001214"
     CDS             299..1960
                     /locus_tag="IWM59_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9979260.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IWM59_001215"
     CDS             complement(2030..2377)
                     /locus_tag="IWM59_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9979261.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IWM59_001216"
     CDS             2461..3402
                     /locus_tag="IWM59_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979262.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IWM59_001217"
     CDS             3403..4446
                     /locus_tag="IWM59_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979263.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IWM59_001218"
     CDS             4452..5522
                     /locus_tag="IWM59_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979264.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IWM59_001219"
     CDS             5512..6465
                     /locus_tag="IWM59_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9979265.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IWM59_001220"
     CDS             6477..7487
                     /locus_tag="IWM59_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9979266.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IWM59_001221"
     CDS             complement(7772..8593)
                     /locus_tag="IWM59_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9979267.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IWM59_001222"
     CDS             complement(8690..8968)
                     /locus_tag="IWM59_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979268.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IWM59_001223"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IWM59_001223"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9979269.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IWM59_001224"
     CDS             complement(11724..12311)
                     /locus_tag="IWM59_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979270.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IWM59_001225"
     CDS             12486..13808
                     /locus_tag="IWM59_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9979271.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IWM59_001226"
     CDS             13963..14739
                     /locus_tag="IWM59_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9979272.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IWM59_001227"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IWM59_001227"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9979273.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IWM59_001228"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IWM59_001228"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9979274.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IWM59_001229"
     CDS             16629..17258
                     /locus_tag="IWM59_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979275.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IWM59_001230"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IWM59_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9979276.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IWM59_001231"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IWM59_001231"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9979277.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IWM59_001232"
     CDS             19445..19981
                     /locus_tag="IWM59_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9979278.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IWM59_001233"
     CDS             complement(20021..21784)
                     /locus_tag="IWM59_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979279.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IWM59_001234"
     CDS             complement(21787..23502)
                     /locus_tag="IWM59_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979280.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="IWM59_001235"
     CDS             complement(24281..24505)
                     /locus_tag="IWM59_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979281.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24705..27473
                     /locus_tag="IWM59_001236"
     CDS             24705..27473
                     /locus_tag="IWM59_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9979282.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27554..27973
                     /locus_tag="IWM59_001237"
     CDS             27554..27973
                     /locus_tag="IWM59_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9979283.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="IWM59_001238"
     CDS             27985..28455
                     /locus_tag="IWM59_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9979284.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="IWM59_001239"
     CDS             28472..29251
                     /locus_tag="IWM59_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9979285.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="IWM59_001240"
     CDS             29238..30059
                     /locus_tag="IWM59_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9979286.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="IWM59_001241"
     CDS             30076..31125
                     /locus_tag="IWM59_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9979287.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="IWM59_001242"
     CDS             31138..32142
                     /locus_tag="IWM59_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9979288.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="IWM59_001243"
     CDS             32775..33794
                     /locus_tag="IWM59_001243"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9979289.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11042 a         5991 c         6908 g         9876 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgt
//
LOCUS       DACDYM010000027        31696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDYM010000027 DACDYM010000000
VERSION     DACDYM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.027"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            363..731
                     /locus_tag="IWM59_001244"
     CDS             363..731
                     /locus_tag="IWM59_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9979290.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IWM59_001245"
     CDS             911..1852
                     /locus_tag="IWM59_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9979291.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IWM59_001246"
     CDS             1921..2652
                     /locus_tag="IWM59_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9979292.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IWM59_001247"
     CDS             2796..4976
                     /locus_tag="IWM59_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9979293.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IWM59_001248"
     CDS             4996..5478
                     /locus_tag="IWM59_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9979294.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IWM59_001249"
     CDS             5494..6255
                     /locus_tag="IWM59_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9979295.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IWM59_001250"
     CDS             6386..7720
                     /locus_tag="IWM59_001250"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9979296.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IWM59_001251"
     CDS             8027..8512
                     /locus_tag="IWM59_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9979297.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IWM59_001252"
     CDS             8505..9188
                     /locus_tag="IWM59_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9979298.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IWM59_001253"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IWM59_001253"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9979299.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IWM59_001254"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IWM59_001254"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9979300.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IWM59_001255"
     CDS             12804..13433
                     /locus_tag="IWM59_001255"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9979301.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IWM59_001256"
     CDS             13485..13817
                     /locus_tag="IWM59_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9979302.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IWM59_001257"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IWM59_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9979303.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IWM59_001258"
     CDS             14643..16016
                     /locus_tag="IWM59_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9979304.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IWM59_001259"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IWM59_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9979305.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IWM59_001260"
     CDS             17071..17511
                     /locus_tag="IWM59_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9979306.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="IWM59_001261"
     CDS             17655..18356
                     /locus_tag="IWM59_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9979307.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="IWM59_001262"
     CDS             18353..19474
                     /locus_tag="IWM59_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9979308.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="IWM59_001263"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="IWM59_001263"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9979309.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="IWM59_001264"
     CDS             20853..21761
                     /locus_tag="IWM59_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9979310.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="IWM59_001265"
     CDS             21796..22068
                     /locus_tag="IWM59_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9979311.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="IWM59_001266"
     CDS             22079..23125
                     /locus_tag="IWM59_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9979312.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="IWM59_001267"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="IWM59_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9979313.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26807)
                     /locus_tag="IWM59_001268"
     CDS             complement(26136..26807)
                     /locus_tag="IWM59_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979314.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26800..27294)
                     /locus_tag="IWM59_001269"
     CDS             complement(26800..27294)
                     /locus_tag="IWM59_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9979315.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="IWM59_001270"
     CDS             complement(27314..28534)
                     /locus_tag="IWM59_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9979316.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="IWM59_001271"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="IWM59_001271"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9979317.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30610..31692)
                     /locus_tag="IWM59_001272"
     CDS             complement(30610..31692)
                     /locus_tag="IWM59_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9979318.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10548 a         5659 c         6786 g         8703 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggtaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtgg tttttttcgg
    30601 tggcaaaaat caagaattct ttctaagatt ttccatgata tcgaccgttt catttctcat
    30661 cttatctgtt acatgcgagt aagtatccat cgtaattgaa attctgctgt ggcccaatcg
    30721 ttcagagatt tctttcattt ttgcgccatt ttcgagaaga agtgtagcat gagtatgtct
    30781 gagagaatgg aagttaaaag agagggagag tgcattcgat atcctcctag tattccattt
    30841 cactacactt ggcgtaacta gctcaccgtc ttccttcgta catactgcat ttgaatcaat
    30901 gtagagcttt ccatacttca ttcgattttc taattgttgt ttcttatgtt ttttcagaat
    30961 tgctagcaag gtttgtccaa taaaaatcgt tcgattggaa ctgcttgtct ttggcgtacc
    31021 atatacccat gcgccgtcat tctttaccat ttgtttctct acagtaattg ttccatttga
    31081 aaaatcgaca tcatcccacg tcagaccaca aacttcgcca acgcgcattc ccgtataaaa
    31141 tccaatattc aaaggaatat agaaaggatg tccttcagga gtaatttcta gcatatgatc
    31201 aaagtcctca agagaaatga tttttagatc ttttttagtc gttggtcgtg cttcatattt
    31261 tggtattttt acatacagca taggattttg cttgattaat ccccaaggat aaaccgccat
    31321 attcagcgca ttcttaagga cagagtgagt aatagtcatt gttttcttcg agtaaccctt
    31381 tttaaattca gcattgatga aattttgtaa aagagcaggg gagagatccg taagtttttt
    31441 ctttcctaaa taaccgttta tatgattttt gatggtaaat cggtagtttt cataggtatt
    31501 gtattttaga tttagtttaa cgtattcctc catccaaaaa tcaaggtatt gttttactcg
    31561 agtatccgta cctaaaaagt attgtcctgt ttcgtcaata tctgataaaa cttttcgtaa
    31621 agcagcttcg gcctctggtc gggtgtctcc gccaactttc tccacttttt ttcttgagcc
    31681 atcatcattg atatct
//
LOCUS       DACDYM010000028        31255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDYM010000028 DACDYM010000000
VERSION     DACDYM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.028"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(475..837)
                     /locus_tag="IWM59_001273"
     CDS             complement(475..837)
                     /locus_tag="IWM59_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979319.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(911..1291)
                     /locus_tag="IWM59_001274"
     CDS             complement(911..1291)
                     /locus_tag="IWM59_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9979320.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1373..2548
                     /locus_tag="IWM59_001275"
     CDS             1373..2548
                     /locus_tag="IWM59_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9979321.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2625..3209)
                     /locus_tag="IWM59_001276"
     CDS             complement(2625..3209)
                     /locus_tag="IWM59_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9979322.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3410..4408
                     /locus_tag="IWM59_001277"
     CDS             3410..4408
                     /locus_tag="IWM59_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9979323.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4520..5029
                     /locus_tag="IWM59_001278"
     CDS             4520..5029
                     /locus_tag="IWM59_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9979324.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5059..5718)
                     /locus_tag="IWM59_001279"
     CDS             complement(5059..5718)
                     /locus_tag="IWM59_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9979325.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5720..6484)
                     /locus_tag="IWM59_001280"
     CDS             complement(5720..6484)
                     /locus_tag="IWM59_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9979326.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6492..7169)
                     /locus_tag="IWM59_001281"
     CDS             complement(6492..7169)
                     /locus_tag="IWM59_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9979327.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7206..8591)
                     /locus_tag="IWM59_001282"
     CDS             complement(7206..8591)
                     /locus_tag="IWM59_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9979328.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKIPKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8801..8885)
                     /gene="ffs"
                     /locus_tag="IWM59_001283"
     ncRNA           complement(8801..8885)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWM59_001283"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8908..9423)
                     /gene="tadA"
                     /locus_tag="IWM59_001284"
     CDS             complement(8908..9423)
                     /gene="tadA"
                     /locus_tag="IWM59_001284"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9979329.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9530..10387
                     /locus_tag="IWM59_001285"
     CDS             9530..10387
                     /locus_tag="IWM59_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9979330.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10468..10803)
                     /locus_tag="IWM59_001286"
     CDS             complement(10468..10803)
                     /locus_tag="IWM59_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979331.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10870..12240)
                     /locus_tag="IWM59_001287"
     CDS             complement(10870..12240)
                     /locus_tag="IWM59_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9979332.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12318..12854)
                     /locus_tag="IWM59_001288"
     CDS             complement(12318..12854)
                     /locus_tag="IWM59_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9979333.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13163..14644
                     /locus_tag="IWM59_001289"
     CDS             13163..14644
                     /locus_tag="IWM59_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9979334.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14747..15454)
                     /locus_tag="IWM59_001290"
     CDS             complement(14747..15454)
                     /locus_tag="IWM59_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9979335.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15533..15871)
                     /locus_tag="IWM59_001291"
     CDS             complement(15533..15871)
                     /locus_tag="IWM59_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9979336.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15982..16944)
                     /locus_tag="IWM59_001292"
     CDS             complement(15982..16944)
                     /locus_tag="IWM59_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9979337.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16959..18290)
                     /locus_tag="IWM59_001293"
     CDS             complement(16959..18290)
                     /locus_tag="IWM59_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9979338.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18298..18978)
                     /locus_tag="IWM59_001294"
     CDS             complement(18298..18978)
                     /locus_tag="IWM59_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9979339.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19197..19676)
                     /locus_tag="IWM59_001295"
     CDS             complement(19197..19676)
                     /locus_tag="IWM59_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9979340.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19803..21482)
                     /locus_tag="IWM59_001296"
     CDS             complement(19803..21482)
                     /locus_tag="IWM59_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9979341.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            22030..23532
                     /locus_tag="IWM59_001297"
     CDS             22030..23532
                     /locus_tag="IWM59_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979342.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23545..24207
                     /locus_tag="IWM59_001298"
     CDS             23545..24207
                     /locus_tag="IWM59_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979343.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24308..24511)
                     /locus_tag="IWM59_001299"
     CDS             complement(24308..24511)
                     /locus_tag="IWM59_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9979344.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24622..25806)
                     /gene="iadA"
                     /locus_tag="IWM59_001300"
     CDS             complement(24622..25806)
                     /gene="iadA"
                     /locus_tag="IWM59_001300"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9979345.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25963..27702)
                     /gene="yfcC"
                     /locus_tag="IWM59_001301"
     CDS             complement(25963..27702)
                     /gene="yfcC"
                     /locus_tag="IWM59_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9979346.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(28475..29089)
                     /locus_tag="IWM59_001302"
     CDS             complement(28475..29089)
                     /locus_tag="IWM59_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9979347.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29695..30072)
                     /locus_tag="IWM59_001303"
     CDS             complement(29695..30072)
                     /locus_tag="IWM59_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979348.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(30372..31253)
                     /gene="galU"
                     /locus_tag="IWM59_001304"
     CDS             complement(30372..31253)
                     /gene="galU"
                     /locus_tag="IWM59_001304"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9979349.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         9316 a         6485 c         5209 g        10245 t
ORIGIN      
        1 ctctttttta aaagtttttt tcgatttcgt ttttcgaaat ctttcagcaa ctcatttatc
       61 ataacttatc gtttgttatt tgtcaagaac ttttttgaag tttttttcga tagaatcaag
      121 cttcgcaagt ggttcttttt taacgactca gttatcttat catgtcgtta gtaacatgtc
      181 aataactatt ttttaaattt tttagaaatg tgatataaca tccacttcag aatcaacgct
      241 gttaaattta tcatgtattt cttccttggt caactataaa ttaagcatta ctttgtgaaa
      301 tgatttttcg atcaattatt attattcgtt atacaaccgt tttcaacata ttttgaaaat
      361 aaaatgcact aaaaaactat ctttagcaga acatttcatt gtattttttg gaccaaacaa
      421 taaaaaaaga agtttgaaaa aaattcaaac ttctttgttc gcttaatctg tattttagaa
      481 gaaaggcact ccctctactt cgtaagttcc aagaacagag cgcatttgac ctggttcttc
      541 ccaaatatat ccttcgccta attcttctct ttgattcttt tgttcagttt caaagacaaa
      601 caacggaaca ttttcctctt taatatgtgt atttgtcaat tccatcaaac taatcttgct
      661 aacatcgata tgaactattt cttttaagaa attaagtata ttcgctaaac cagtctttcc
      721 ttcagaaagt tccgctaacg caaattccaa cttgtcatca attgaatgga ccaaaaattt
      781 tttagtacca tcctccaaat gcaacataat tgtaccagca actcttttgc ttgccaaaat
      841 ttccacctcg atcaacttgt tctatggctt acataaaaat tctagcacat tttcagaaaa
      901 aatacacttt ttatttctta ttttctttcc gttctagttc agataatgct tcttctatcc
      961 aaataggtaa ctgctccgca atagtggtaa atccgatctt acgattttta ttcgtgaaat
     1021 acctttttct gcgatatgga acagctggat ttgttttttc taacgttctt aaagataaac
     1081 tgattttttt ggaatattca tcaatgtcga tgatctgaac tgttactggc tgtccgactt
     1141 ttaacaatga atggatattt ttcgtataac cggattgtac ttctgaaaca tgaataagtc
     1201 cttgcgtctc gttatcaaga gaaacgaatg cgccataagg ctgtatccct gtcacttttc
     1261 cttcaattat ttgtccaatt ttataagtca tctacttccc tctcttcgca tttatcatta
     1321 tactgtaaat agctcaaaaa aagaagcgac gaaaatgact ggaggtctta agatgatcga
     1381 atttgataaa cagatttcaa gaaaagatac agaaagtgta aaatgggacg caattgctga
     1441 gacatatcaa atggatgact tacttccttt gtgggtagca gatatggact tcttgtctcc
     1501 cggtggggta gctaaagcgt ttagtgaata catcaaacat ggaatctttg gttatgccac
     1561 tctgtctgat aaactatatc aggctatcat tcattgggaa cgccaacacc atgctgtgga
     1621 gttgacgaaa gaaaatatcg tttttacaag tggtgtgtta accagtttag ctgcagcagt
     1681 acaaactttt acaaaaccag gcgattctat tttgattcat gacccagttt atcctccatt
     1741 ttcctctatt atagaaacaa atcaacgtca catcgttcga agctctttgc tagaaaaaaa
     1801 taatcatttc atcatggact tgtctgacat ggagaaaaaa atcgaagaaa acaatgtaaa
     1861 agcaatgatt ttatgcaatc ctcacaatcc tggagggcgt gtttggagca aagaagaatt
     1921 gaaacagtta agcgaattgt gtttaaaaca tcaagtgatt ctgtttagtg acgagatcca
     1981 tcaagattta actttattcg atcatacatt tacttccatg ctaacgattg atcctaattt
     2041 agatgatcta ctagttgctt ttacttccgc aaccaagact ttcaatctag ctgcaatcaa
     2101 aaattcaatg gtatttatca aaaatccaga gatgaaagct gattttgaaa aacatttagt
     2161 gatgaaccaa cagcacgaaa tcaacacttt tggactgatt ggaacccaag cggcttatga
     2221 gacgggtgga gaatggttaa gccaactgat tccttatcta gaaaaaaatg ttcaaacagt
     2281 taatcagttt tttgaacagt atcttccgaa agtgagaata atgaaccctg aaggaactta
     2341 cttaatgtgg ttagatttta ccgcctatat ttcagacgac caagaattgg agaacactct
     2401 tgttcggaaa ggaaaagtcg tattaaatcc tggcatcacg tttggtcctt ccggtcatgg
     2461 gcatatgcgc ttgaatattg cttgtccaga agaaacttta ctggaaggac ttaatcgaat
     2521 caagtatgct ttagagacaa tcgattaaat catttattca aaataaaagg acgagacctt
     2581 actaggtttc gtcctttttc ataattactc cgtaccatac caaactattc tgaaatttca
     2641 attgtttcaa tcactacatc atgtacaggt cggtcttgtg gaccacgttg tacatttgcg
     2701 atttcatcta ctgtgtccat cccttctagt acatgtccaa agactgtatg tcggaaatct
     2761 agccaaggag ttccgccacc tttatacgct tcaatgattt ctgatgggaa tccagcacct
     2821 tctagttgtc caagcatatt agctggcaca tttgtgttat ttacaataaa gaattggctt
     2881 ccgtttgtgt ttggacctga atttgccata gataaagctc cgcgaaggtt gaacagttca
     2941 cgactgaatt catcttcaaa actttcgcca taaatacttt cgccacccat tcctgttcca
     3001 gttggatcgc cgccttggat catgaaatca ggaataacgc ggtgaaaaat cacgccatca
     3061 taataacctt ttttggaaag ttcaatgaaa ttttgaaccg tcttcggtgc tagttcaggg
     3121 aataattgaa cagtaatgtc accacggttt gttttgataa cagcttttgg tcctttttca
     3181 ctttttaaat ctagttgtgg gaatgccata ttgtttcctc ctaatatttg catacttcat
     3241 aataataata acataatacc taacctctcg taaaaatact agatttgttt tcttttattt
     3301 ttcatcacta gatacattct ataacttacc gtttcgattt cgaattcaaa catttttgag
     3361 aagcattctc aattaaattc tgctatagtg tgaaaaggga ggaattttta tggaaatcta
     3421 tgacattaca atcatcggtg caggtcctgt agggatgttt gccgcttttt acgcaggaat
     3481 gcgccaagct aaaacaaaaa tcattgacag tctacctcaa cttggtggtc aattagcaac
     3541 tttgtatccc gaaaaatata tttacgatat tcctgggtat ccagcaataa aagcaagtga
     3601 attaatcgat caattagaaa aacaattaac tacttttaac catacattcc atttaaaaga
     3661 agaagttctc tcgcttactc gtgaagacga agttatcgag attactacaa ataaaggcat
     3721 ccattattcc aaagcagtca ttttagcttt aggaaatggt tcattccagc caagaaaatt
     3781 gaatttagat aatgctgaat catttgaaaa ccacggattg gattatttcg tgaatgattt
     3841 gatgaagtat gctggaaaaa aagtcgctat tgctggaggt ggtgattctg ctatcgattg
     3901 gacgttgatg ttagagccta ttgcaagcga agtttacttg attcatcgtc gcccggaatt
     3961 tcgtgcgcac gaacatagtg tatcacgctt gaaatcttca tctgtcaact tgcttactcc
     4021 ctatctcatc gatggattat caggcaataa tggggaactg acagatatcc ggttaaaaaa
     4081 agtgaaaagt gacgagacga ttgatttgat gattgattct ctgatcgtca attatggatt
     4141 ctcttctaat ttagaacatc tttcttcttg gggacttgat agtaccagaa atgccattac
     4201 tgtaaaatca gatatgtcta cttctattcc aggcgtttat gcagctggtg acatctgtac
     4261 ctatgaagga aaagtgaaac tgattgctac tgggctcggt gaagcaccta ctgcagtgaa
     4321 caacgcttta cactatatta atccaaagga acgaactcag cctggtcaca gcacaagctt
     4381 atacgataaa aatcttcgtc ctagttgatt ttttatatct ctttgcattt caagttaaac
     4441 ccgaaagcag tttcttgcat ttttttcatg ttccgttaac atgaaataga gaaagaaaaa
     4501 ataaaggggg agcaataata tggtcgtcaa aactgagact ttagaagaaa aggcaagaga
     4561 actattgaag gaacgtggcg taactgtaga agatatcgca caacttgtca tgtttttaca
     4621 aaaagactat atttctgatt tgactttggc ggattgtact gaaagtgtca acagcgtgct
     4681 aacaaagcgt gaagtacata acgccattat cactggaatc caattagata tattagcaga
     4741 aaagaaaaaa ttgatgagtc cgttgcaaca tattatcgaa gatgacgaag gcctttacgg
     4801 tatcgatgag atcctagcct tgtcaatcgt gaatgtttac ggtacgatcg gctttacgaa
     4861 ttatggttat atcgacaagg tgaaacctgg tattttaaaa gaactaaaca gccacgatgg
     4921 cgttcatgtc cataccttct tagatgatat cgtcggagcg atcgccgctg ctgctgccag
     4981 tcgattagca cacaaagaac cagaaaaaag aagtcgcctt actcaataat tcaacaaaaa
     5041 aagaaagaaa acgtctattt agacgagttc tttctttttt gtttgtttca agctattttt
     5101 tcctaagaaa agaaaaacag caaaaaataa ttctaataat acaaaaaata agataaagct
     5161 atgcatgaaa aattcttctg gaagtacatt gatgattgga tcagtaacgg gatcgaataa
     5221 ccagtcatcg ttgttgaaaa acgtttcatg aaaaaggata aaaaagcgat caaatcctat
     5281 catcatgaaa aaaccgaaaa caactggcag taacatgccg atttgaaacg gacgaatcaa
     5341 ccgccaaagc cgatcattct tttgcaaata tttgataaac aaaatactcg ggatcaaagt
     5401 gatgaaaaac acagcataat ccactaaaaa caaaattttc acttcataaa agtgatgggc
     5461 tccgctagca gaaactggga aatctggcaa gctgaggatg gttttgaagg gattattgag
     5521 ataattcatt aggtgatcaa aatttttcag taacgtctct tgatctaaag aagtataatc
     5581 caaaatttgc aaatgtccga tatcaaatac gtatagtggt cgaaagttga tcgtcacacc
     5641 aactgccaga ctgatcagtg tcagaaatag gctgaacaat ccagcatatt ccatccactg
     5701 ccatctcgaa tttttcatat tagaagctcc attcatccag tgattgtttc acataggtag
     5761 ctgggatagg caacccaggt acatcttctt cttttgtaaa acctgataaa acgagcaaag
     5821 tatctatatt attgcgtatc ccagcttgga tatctgtttc atagttatct ccaaccatga
     5881 ttacttcttc tttttgcagc cctaatactt caacggcttt atccataatg atggcttctg
     5941 gttttccgat gtagatcggc ggcgtttggg ttgctgtctc tacaaaagag atgactgacc
     6001 ctgctccagg aagaaggccg cgttcagtag gtatattctt atctgggttc gttccaatga
     6061 aagtagcacc tttttgaata gcaagtgttg ctttaactac tttttcatag gtcagataat
     6121 tatctaagcc taccactaca tagtcaggag cctcttcttc ccatgtaaat ccggcagcta
     6181 aaatcaagtc aatcaatcct gcttctccga taacatagac ttttttccct tttccatctg
     6241 ctttcataaa atcgattgtt gccaaagtag ctgtgtagac tgtctctgga gctacatgaa
     6301 tgtcaaactc atctgctaga cgattagcta ctgtttctgg tgacttagtc gtgttattgg
     6361 taacaaacaa gaaaggtaat tttctttttt gcaattcttc cacaaatcgt ttaccggccg
     6421 gtatgggttg tgtcccacga taaatcgttc cgtctaaatc aattaaatat cctttatagt
     6481 ccattcatcc actactcttc cctttttctg atcgtaaatg ctgtttttgt tttttctact
     6541 tgtttaggtt gttcttgttt ttttactttt tcagaggttt ttgctgactc tgtttttcga
     6601 tttttaataa ccggtttttt gcgattagat gcgactggtc cttttttctc atcgatatac
     6661 gcacgattat ggtttttttt cttttttcgg cgctgagttg tcttttcttt tttaccgccg
     6721 attctctcga tgacaaaata cgcacaacca aaattgcaat attcgtaaag atagtcttcc
     6781 aaggaatcga ttcgctgatc tggatgcgcc cgtcgattat ccgcttggaa aaatcctttt
     6841 aggcgtagtt gttcataacc ccaatctcct acgatataat catatcgggc tagtacatca
     6901 ctgtaacgtt ctccaagctt ttctgcatta aatccttcac gataatcgga cacaagccga
     6961 taatgtcttt cacctatcaa aaaattactg tcatctaaca gtgtcactag ctcgcctttg
     7021 actttttctg ctttaggctc ctcaacgatt tcttccaaag cagctgtcag ttcttcggtg
     7081 actgttgttt cctgttcagc tgatgatttt ttcttagcaa tttctgtttt atgctcaacg
     7141 ttctgctgtt cattttgttt tgcagacatg cgttcaccac ctatcttttt tattatacct
     7201 tgtttttatt tgattgggta gtgagctttc aagtaatctc ctaccacact tcggataaat
     7261 tcagggaaca aaaattcatt ttttccagca atctcaatcg ttggaaaaaa tggaacaaac
     7321 atcaaatgat ctacggtagt aaaagtataa tttttattca gatcgattgg cttgttcttc
     7381 caaaagactt gatggttaat agaatcatat gtgattccat tatagaccat ctgcccaaaa
     7441 atcttaccgc gaaatcccat tcccttcata ggatagttac gtaaaaagtt acgatttttt
     7501 tctatttcta aaactaagcg gatcagatca tttccaccca atgtgacatt taacaagtgc
     7561 atcggatgcg gcaacgcagt gtgaagctga tcctgattga cccgcccttt tggaatttca
     7621 gttaaaaaca gaccactgtt taatattgct gcttccgtct ttcctcgttc tttcaccgcc
     7681 tcaaaggcta cttggataaa cgaatgctca ttgaagatat ctagactcag agaaaatggc
     7741 aaatctgcta cttctttttc ttttaataat tcatgcccac gttctagata actatttatt
     7801 tcttgttcat cttctgtaaa agctgtcatt gtttcggtag ggatggcttt ggctgaatgc
     7861 ttagggattt ttttctcttc atcgatggtt aaatggactt caccgacata ctgcccgtac
     7921 tttccagcgg ctgctatttg gacatcattg attacgtgac cttctctgaa taaatgatgg
     7981 gtgtgcgatc ctaaaataac atcaattgct ggtatttctt ttgcaatttg gaaatcatcc
     8041 tgaatcccga gatgactcat caaaactaaa acatccactt gtgaacgtaa atcttcgact
     8101 aattctggca aaatatcata aggattccgt atgtcccagc cattcggatt ataagtaagc
     8161 ggaaatggag cagtaaatgc aattagtccg attttcgtct ggtgtttcgt tgtaatgatt
     8221 ttatatggtt tcgtccatgc cggtggctgg agcgtatttt tatcgaataa gttatccagg
     8281 atgatatcaa actcagcatg atcgtataaa tgattcaact cttctttgga atttcctacg
     8341 ccttcattgt ttcctatcgt cgctgcatca taaccaactt cattcatcag caagatattt
     8401 tcctgtccat tcgttgcttc tgacaatgga tgccaacgat ccacaaaatc acctagatct
     8461 actgcaatcg ttgtcgtttt ttctttcttt tcgttttctc gtttacgttg atccaaaaac
     8521 cttctgatcc ttggccaatt ttctaaatga gaatgcagat cattcgtatg taaaatcact
     8581 attttttcca tgtaccctct cgcctctctt tatctttatc ataccatagg cagctttttc
     8641 ttccattgaa acaagctcta tttcagagtt tttcctgcct tgaagcaaat tgtataagta
     8701 caatattcga taaatcagtc tggctaaaaa atcaggaaca ttcattttta cagtactata
     8761 aaaataaaaa aaacagaccc gaaagtctgt tgtcgtttca ttataatcag acacatggcg
     8821 gaacctgctt agtgctgctt ccttccagac ctgacacgct tcacaagccc accattgtcc
     8881 tagcctttcg gcaaagaata gtatacctta tttttcattc gttgaccagt ctttttttaa
     8941 tttctttttt cgctggcgga gctttcggaa aaagtcagtc aaaatcatcc cacattcgtc
     9001 ttctaaaatg ccaccttcta catatgcttg gtggttaaac cgctcgtcct ccaaaagatt
     9061 catcaaggta ccggctgttc cgccttttgg atcatacgcg ccgaaataaa cttctgctac
     9121 acgagacaaa atcatcgcgc cgctgcacat aggacaaggc tctaaggtaa caaaaagtgc
     9181 agcatcttcc aagcgccagc ttcccacttt ttcacaggct tccttgattg caagcatttc
     9241 tgcatgtgta gtagcatctt gcgtggtttc tctaaggtta tatcctcttc caatcacttt
     9301 tccatccaat acaaccactg ctccaatcgg aacttcatga agcatttctg ctttctttgc
     9361 ttctgccaat gcaaggcgca tccatttttc tttttctact gcatcaagat ttgtatttac
     9421 caattaccct tccctgcctt tgttttttct tgttttactt tagcatatga taaaaaggct
     9481 gtaactaact tttttttcta tggtaaaata aatacaagag gtgaacaaga tgaaaatgga
     9541 taaattactt gctctttacc cacaagcaca gaaaaaaaac tctccagcat cagaagcgga
     9601 tatcctttct tttgcaattg atgatgcttt tatctggatc aaacaagatt cattatctca
     9661 acaagaaacc agccttttga aagcgttatt ccctgtaatc aacgatcaaa aaaaacaccc
     9721 atggtatcac tatcttttta aagatgcttc ttgtgttcaa gaaaaaagct ttcgtttgat
     9781 ccagcttcac gtcgaatcaa aaggagaatt cttaaaaaaa gaatggcaag atactatttt
     9841 ggaattattt cgctattctg ctgatttctt tttttataca gacacagatg cgctcttagt
     9901 cgaacaaata aaccgtgatt atcttgatgc tactgaaatc aacggtattt ttctatcttt
     9961 agatgctgat tttgatacga caacgtctgt attcgttggt tctttccatt cacctggcac
    10021 cgacctcgtc cctttgtttg ctgaagaaag aaaaattttt ttaacagagc aaaacaatct
    10081 ttatactcac agccgtactt tttctatgca agatgtggct ttacattact atacaaaaga
    10141 tactgtaaaa gatagtaagt tgatcaaaag ttacatagaa ctgatcaaac aaaatgaaat
    10201 ggaagtgatc attcaagaat tatggaatga attaggcaat gtcagctctg ctgcaaaaac
    10261 actgtttctt catcgtaata ctctaaaata tcgaatcgaa aaatttcaag aacagtctgg
    10321 tttcaatcta aaaaaagcca acgatttact gttttgctat cttttattgc ttcatgaaca
    10381 taattaaaga tagattaaag gggttgtgac aataattttg tcacaacccc ttatttcgaa
    10441 tgaatagtgt tcaaaagcta gtttctctta catcattttt tcaacaaaat gcattagccg
    10501 gtcttttaca tcttcggttg aatctttgat tgaatcacca taaacagaga ttttcttttt
    10561 tcctgatttg atttttgcta acattcccat caaagaatcc aagtcactat ctgaaagacc
    10621 atctacgatt tctagtaatt tttcatttcc atcaaatttg tcatagacga atttttttac
    10681 tttcgtccga gtagtcatat gatggatctt ttccattact tttcccgata caacgactgc
    10741 tgtcgctaca ctggcagcgg ctgctacgct taatccgatt gttacttttg ttgatgtttt
    10801 cataagtatc agctcctttt tatctatctc catcatagca tagacaaaaa aataaacgaa
    10861 ctaatccact cagtgaaatc tcaatccttt agggaaaaag ttctttactg ttccttttac
    10921 ttgtttccca aatccgacag gtatcttatc taatactagc aaaacccatc cttgattccc
    10981 atcacgttga aatgtttctc ctgctgtgta agattgccat tccttttgag tgatcggcaa
    11041 gcaaggtata ttctctatct tttttgtagc aagcgctaat gcatagctag gttcaaatcg
    11101 attctttttg aaatctccta aatgcaaacc tgtacgtacg acttttaaac catcgagaga
    11161 aggggctaac tcaggaactt cccaaagatg gtcgttgaac actaaaagtc ttccagtagc
    11221 ttcataatga aaatcatttg aaaactccgt ccagagtttt tcctgctctt ttgtcatttg
    11281 cactttactt ttttttcgtg tcttcttttc tttatgcatc tgattttgtc catgaaaagt
    11341 cagctttgca acaaaatgtc cttcaccctg atctttatga ggccatatac gtattgtttt
    11401 ttccaaacca gctacgcttc cccattcaga acgtcctgaa ctaacggatt gagtcaaagg
    11461 aatctcctca atcgttactg gataattttc tacaagccag ctaatgatct cttcattttc
    11521 ttctggtgca aacgtacaag tcgaataaat cagttgtcct ttatttttta gcatttttat
    11581 tgctgaagac aaaatctctt gctgacgttt ttgacagtat agcggagatt cttctgtcca
    11641 ctcttttatt gcatttggat cttttcggaa cattccttct cctgaacaag gagcatcaac
    11701 cacgatccga tcaaaaaagc ccgaaaaatg agggacaagt tctgcgggtg catgatttgt
    11761 cactattgcg ttcgaaactc cccatcgttc tatattttct gataaaatct tcgcacgttt
    11821 cggaaagatc tcattcgtca ctaataaccc tttccccttc atttgagcag ctaattgggt
    11881 tgatttacca ccgggagccg cacacaaatc caaaactttt tcaccgggtt ttgctgcagc
    11941 agctgttcca acgatcattg cacttggttc ttggctatat tcatacccag cttgatggag
    12001 gaaagacttt ccattgacag tgccaagaaa tccctcattg ctgtagggag cgggttgtaa
    12061 ttcttcgcta tggtaagttt ggaccatgtc caatcctgcc ggttttagtg gattccaccg
    12121 aaatcctttt ttcacgcttc cttgttcaag cgcagaaaaa aaatcggatg cttcttcgcc
    12181 taaaagaaga cgatattttt tgataaattg ctgtggtaac gttgtcgctt cctccttcat
    12241 tccaatgttt cgttagacat tttacaatct tttttacgaa aaataaaggt ggctgatcta
    12301 ctttttcatg ttgctgccta aggtgactgt tcctttaaga tattttccaa gtattcatcc
    12361 gtcacctcgt ttttatccca aacagcgttc atcatttcaa gaaaatcttg agaagaatca
    12421 tctgagcgca aacttcccaa tgcattaagc cataccaatt caagcgactt ttcttctttg
    12481 atcttcgtgt gacctttgac atatgagaaa aatggccgct taaatagcga atccatgtct
    12541 aaatcagatt ttgcaatgat ttcacgaata ctttcatgga tagaaaaaaa atctttcaat
    12601 aagtagacaa tgcttaaaac atcatcggaa ctgcattcag ctaagtagtt ttcaaaataa
    12661 acagtgattt cttctaatat cttctctttt aacccgctga ttgatttgaa atgtgtataa
    12721 atcgggtatg tagaacaacc gcagaactct gctaactctc ttgcagtaat tgacttcatt
    12781 ccatattctt ccacgtatac catacttttg aacagcaaat attctttact ataatttgtt
    12841 actctcccca tcattcttac ctccgtctct tttcatctac ccataaataa ataacataaa
    12901 taattctaaa aatattattg ataattatca aaacatattt agttatatcc attaaaaatc
    12961 aatttttccg ccttaaaaac aagaatatct tgacaaaata ccctaggaaa taaacaatag
    13021 aataaacaag gtaacaaaaa aattaaataa cgttatataa aaaagaaggc gacactctgt
    13081 gagaataaca cagactgaaa tgactttcca ctcacagttt ttgtctaact atatacaaaa
    13141 acagaataaa aaggaggtat ttatgctaat tgaagcaaga aaaaaagagt tacttgttct
    13201 acaaatggtc attcaaaaaa ataattgttc tttacaagaa ttatccaatg acttacatat
    13261 tccaaaacgg acaatcaaag aactgatccg aaaaataaat gtatcactca agcaattttt
    13321 attgattgat gaatttattt attctaatca taaaggagag atccaaacca aagaaaccga
    13381 tcaaactaaa aagttagctg ttttttctaa gttaaaatta tgctacctaa aagaatccaa
    13441 tcgttttaac tatttgatgt tactaatcaa ttatccacaa actgctgtac caaaaaaata
    13501 tttacttgaa caactttata tatcgccctc ctatctcgaa aaactgacaa aacagctcaa
    13561 tcattcctta aaaaaattcc atttacggat cctatctgta aaaaattgct gtactcttga
    13621 aggaaatgag ttatctatcc gtctctatct ttactttttt ctttcagata catttggagg
    13681 taacgattgg ccattttcct cacttgaatt aaatgcacta aaaatgaaca gtacagatga
    13741 gcaaatagaa aaaatttcaa aacaagaaaa aaatatctac ttgctgttaa ctttattcat
    13801 attacgaaac aaacaacagg catattttcc aaaaacttct tctgaaatca ctgaattgat
    13861 ggacttattg cagcatacac atgattattc agaatacttt acttcttttt cagtgagtcg
    13921 cccttgtgaa gagacagaaa cagagagagc ctactttaat tattttttct attgtttttt
    13981 tccaaatcaa atctcttttc gacaaaaaca ggagattggt tttttatttt ccacaagcaa
    14041 aaaccttatt tgtcgacaag tcggtcaatt gatgacttac ttaactcaac agtttcctct
    14101 caaccattta gaaaataaaa taccaattta tttttatttt cttgtgttgg cagttgtttt
    14161 tttcgaactg ttgggtgaat ccagcagtag tttttctaca ctttgctttc cagaagccac
    14221 gtccaatcat tctagttctt ttatacagga aaagaaatta agagaaaaaa tcgatacaat
    14281 tttttcaaaa aatggggaaa gaaaccaaat cagtcaatac cttagtcaat tgatccaaca
    14341 cttactacaa acagaaacga aagagcagtt actcatccat ttgcagataa ccaaacagtt
    14401 tacaggaaat tatcatcttc aaagccattt gcagcagtta tttcgcaaag atgctgttaa
    14461 aattaccgca tattcagaac atgctgattt gatcattaca gatcagcatg agaaaatttc
    14521 agatgaaacg gagactttct atctggatac aacgcacaat acagtgttat ttggcaagtt
    14581 aataaaagcg gttcatgaac tgtatatgaa aaaaggttat gagaacaaaa caaagaggat
    14641 gtgacaaaat tcttgtcaca tcctcttatt ccaaataaat ggtattcaat cagctaactc
    14701 tcggtattag ctcagatcaa agcgctgatt tcacaacttc tttattttaa tctcttcggt
    14761 tgccgccgaa caataagatc aagcgcaaca gctgcaggaa tgtcgaaagc gctgctgcaa
    14821 cataagtcag tgctgctgca aataatacgt gtcttgccat cggtacttct tcttccgtca
    14881 gtagtccgcc ttcactcaaa atttggattg ctcgccgaga cgcgttaaat tcgactggta
    14941 atgtcaccaa ttggaaaagt aatgctaaag aaaaagcgaa aatcccgata ttgattaacg
    15001 tagagttcca gctaagcaat acacctacca taatcaaggg gaacgaaatc atcgaaccaa
    15061 agttagctgc tggaacaagt gctgcacgaa tcttcaatgg gacataaccc gaagcatctt
    15121 gtaccgcatg tccgcattca tgagctgcta cacctatcgc agcaactgaa gtagactgtg
    15181 cagttgcttg agacaaactc aacatcttat tacctgaatt gtagttatct gtgagatctc
    15241 cggcaatttg ttgaacaccg acattattta tctgttgact ttgtaatata tattgtgctg
    15301 cttgtgtacc agtcacattg tttttgcttc tcacttgatc gtattttcgg aaagtactat
    15361 tgacgtacgc tgacgcaatt ccggaaataa ctaatccaag aatgaccaaa agaaatgtcg
    15421 gatctaaaaa accatacatt ccataaccat acattttcgt ttcactcctt ttcgtttact
    15481 ttattttatc attagtagta ggaaaagaat gtgaaaagac cttgaatatt ctttaattct
    15541 ttttcacaaa ttccgatttc agtttcatcg gtccaaatcc ttctaccttg caatcgatat
    15601 tatgatcgcc ttctactaaa cggatgttct tgacttttgt tccttgttta atggcacctg
    15661 acgcaccttt tactttcaag tctttaatca ctgtcacgct atcgccctct aacagacggt
    15721 ttccatttgc atctaatacc actaatccgt cttctatgac agtttcttgt tcttctttgt
    15781 tccattcgtt cccgcattct gggcaaatca acaagcctcg atcttcatat gtgtactctg
    15841 agccacattc tgggcaattt ggtaatgtca tcttctttct cctttaaaat ctttagtgag
    15901 cactattgta acggaaaaag aagatcttga aaagatacat ttgtgatttt tactacaaaa
    15961 ggcacgtttt cgaacgaagt atcattctgt ttttctgctc ttatacgatt ggcatatatc
    16021 ttggatctct ttatagattt cctctacttc tgctaaatct tttgcattcg caccacttgc
    16081 taatggatgg cctcctccat gatggcgttt agccaattca ttgatgatcg gccccttaga
    16141 acgtaaacgt acgcggtaat accctgaagg ctgctgaaca aaaatccccc aagccagcac
    16201 ttcgtcaatc actccaggaa gtgaaacgat cgaagctgtc tctgaatcat cgattccata
    16261 tttatcaaga atttcttgcg gaaggattat tcttcctgca ccattttcat ccacctggat
    16321 attttgataa agataaccgg aaagtttagc gactttcatc ggcatttgtt caatctctct
    16381 gttcaaagca cttgcatcaa atggataatt tctcaatatc gaagcaatct ctaatgtacg
    16441 ggacgtcgtg gagggataca aaaagcgccc tgtatctccg acgattcctg cataaagtaa
    16501 ccgtgcagcc ttaacgttca tctttaaaga ttcaggaaac atcaaagcaa attctgcaat
    16561 catctcacta cagctacttg cttttgtatt tacccacaca agatctccat aagggtcatc
    16621 attgggatga tggtcgattt tgatcagttt ttctgccaac tgatatcttt gatcactgat
    16681 tctgggtgaa ttagctgtat cagtcacgat gactagcgct ccttggtaga agacatcttc
    16741 aacttcttgc atttctgcta aaaagcgaag cccttcaatc gtttcaccta cttgacgtat
    16801 ttctttttct gggaaacttt cccgtaatag ttcggctaat cctacttgtg atcctaatgc
    16861 atcaggatca gggcgcaaat gcctgtggat aatgatttta tcgtattttt ttattgtatc
    16921 tactatttct tgctgtactg tcatttcact cctgctttct aagaacgttc cattacttga
    16981 caaacaacga ttgctttgga aacgatggtg ttttcaataa acacttcgat atccagttta
    17041 gcggaacgtc tgccgatttc caatacacgt ggacgaatgt ctaattcgct ttccaattgg
    17101 atcagccgca aataatgtag gttcacttgt tcaatcaaga tatttcgttt ttggttcatc
    17161 accattgttt tttgtgcgac atcagaaatg atttcgctaa gtacgccaaa agaaatggtt
    17221 ccaacgccat tgaccatttg tggtgctact acgaatttga acttagggtt ttccaaacga
    17281 tttccttcac ggtcagtatc aatcgtatgg atatttccag atatctgatc cgaaatcgta
    17341 tctgcaatct gcggttgtcg ctggacaagc tgcatcgctt tcattacatc ttgccgggta
    17401 atcaagcctt ccaatgtcaa gtcatcggaa acgaccggca tgacttctag cccatcccag
    17461 atcatttgat ggctaacaga ggcaatactc attgttttct ttacactgat tggttctttc
    17521 gtcatcaccc gatcgatgat ctggtttggc gatttcccta aaacatcttt tgccgtaaca
    17581 atccccatca aacgatgatg gcggttgacg actggaaaac gcgaatgctg cgtttcttct
    17641 gacaaacgct gataatcaga aatcgtatct gtctgctgca aataatgagt cttttcagct
    17701 ggcatataga tatcactgac aagcatgata tcttttttga tcaattggtc gcttaacgct
    17761 cggttgatca tggttgcgac tgtaaatgta tcataagagg tgcgtaagat cggcatttca
    17821 agatcatcag ctagctttgc aatctcagca gtcgtattga atcctcctgt gatcaatact
    17881 gcggcgccat cttccaatgc taatttttgg acttccgccc ggttgccgac gatcattagt
    17941 gatcctggcg taatatagcg ttccatggca tgtttagtca tcgcgccgat gacgaattta
    18001 ttcaagacct tgtctaatcc tgccgccccg cctaaaacgt ccccttcaat gatcctgacg
    18061 acctcgccaa atgtcaaacg ttcaatatgt ttttttaatt tgcgttcgat acggatggtc
    18121 cctactcgtt gaattgttga aactagtccg acattttctg catctttgat tgcacggtac
    18181 gctgttcctt cactgacatt caattctttt gcgatgcttc ttacagaaat tcgattgcca
    18241 acaggtaaac tttcaatata ttctaaaatc aaatcatgtt ttgtcgccat tttctgccta
    18301 cacttccagc gtttccgaga cgctcattat ttttccttca ctatcttcca gtaactctac
    18361 aaaagattcg ggatcttgtt caatgacagg gaaagtattg taatggattg gaactacttt
    18421 cttggcacga attgtttgcg cagcctttgc tgcatcttct ggccccatcg tatagttatc
    18481 gccaatcggc aagaaagcga catcaatatc gaattcttct gcaagcaatc ctagatcact
    18541 gaaataagca gtatcccctg catgataaat cgtttttcct tctgcttgaa taataattcc
    18601 gctaggctcg cccatatact gaacgatacc gtctacttca tagcttgaac tatgctgtgc
    18661 atgtacgaat tttaccacgc cgaatggaaa cgcatgtttt ccaccgatat tcataccgaa
    18721 tgaatctact cctctgctgt tcgcataatc agcaatctca acaatactga taattgtggc
    18781 ttgattttct tttgcaatag gaatcatatc tcctatgtgg tcactatgac catgagtaat
    18841 cagcaaccaa tctgtcttta cttcttctgc agttaagtct gtcaatggat ttcctgtaat
    18901 aaacgggtca atcatcacgc gttggccatt ttccatttca attgttaaac aggaatgtcc
    18961 atgatacgta attttcatat tttacacctc gcgttttctc tgtatctttt ttattaaaaa
    19021 cgatttcatc acttgcacat caatgaaaaa atatagctgc taaatatatt ttcattggat
    19081 tgatagtaca gtatgtacaa agcaattgta acagataata caggatgcac caagtaccaa
    19141 gcagatataa tctcaagttt gaggtctctg ctctgcttag cttctcttct cgtcccttaa
    19201 ctaattaaat cagcgtaaat aggatggacg atcttttcta aatcggctac ttttatccga
    19261 aggcttctcc cgatttcacc gcttgacaca attacttctt ctttttcttt taaagaactg
    19321 tctgctaaaa aacaataatc aggatgtgcg agttttattc caataggggt gtttgctccg
    19381 tggggatatc ctgtatgcat taatacaaaa tccattccag gaaaaccaac tttacgatct
    19441 ttcgtcactt gtctcgtttt tttgtaatct aagtgatcgt tcaaaggaat caatgcgatg
    19501 atcaccccag ttttgtttcc tttcactacg agagttttac atatcaaagc tggatcaatc
    19561 cctctttttt gtagttcttc tgtgaaagat tcttctacca catgactaaa atcaagtggt
    19621 tgatagctga tcttttgttc ttctaaataa cgttcgactt ttgtttgact catcattgat
    19681 tcactccctt tattctatcg tttattataa agcgagggat gctgtattca aaaattattt
    19741 gtttttcgca aaaaaagagc aagacgagca ctctatagag aggttcatct tactcttttt
    19801 gtttattttt tatttcgacg ttgagtagct tccataatga ctggcaagat cactgggcgg
    19861 cgttttgttt gttcaaataa gtaacggcct aattgatctc ggatgtcttg ctttaatttg
    19921 ctccactcaa aatcattgct ttctaaatgt ttctcaacga ttgtttcaat catattgctg
    19981 ctttctttca tcagatcacg gctagctttt acgtaaacga atccccgtga agtaatttga
    20041 ggttttgaaa tgattttctt ttctcgccga ctgatagtga caaccgctac gaaaatcccg
    20101 tcttctgaca agatttttcg atcacgtaaa acaatgtttc cgatatcacc tatgcctaat
    20161 ccatcaatca ttacattttc tgcacttgta gttcctgcaa cactcatacg cttgttctta
    20221 tattccagca cgtctccgcg tccagtaata tagatatttt tgtatggcat ccctactgca
    20281 tgagccaaat ctgcatgagc agctaattca cgatactctc cttgaactgg cacaaaatat
    20341 gttggtttca tcaaattgat cattaattgc aagtcattag gattggcatg gccagaaacg
    20401 cgcatattct cagagatcaa cttcactgtt ccaccagctc gataaatgat atcttccgtt
    20461 ttcgccacga tggtttccat cgcgatgctt ggcgtagtag tgatataaac caaatctcct
    20521 tcttcaatct tgacattacg gtgtgtaccg tttgccattt tttgcagaga cttgatgggt
    20581 tcacccattc ggccagtttc taaaatgatc agttcttctg gatcatagtt tttcatgtct
    20641 ttcaacgtaa caagcagatc ttcacttggt aattgaagtt tttctaaacg catcgctgtg
    20701 cgaataattc gttcgaaatc ttgaccagtc aagacaactt ttcgttgtgc tttttctgct
    20761 gcatccaata cttgttgcac tcgttgcaaa ttacttgcta cacaagcgac gatgatacgc
    20821 ccttcccaat atttgatcgt atcaaatact tcatctgcta tttgggcttc agaagctaca
    20881 ggctgaggat tttctgcatt tgccgattca cttagtaagg ctaagactcc ttctttgccg
    20941 atttccgcca atcgtgcata atctgtttga tacattggga tagccgtttg gtcaaattta
    21001 aaatctcccg tataaacgat actaccttct tttgttttca gattgatccc cactgaatct
    21061 ggtatcgtat gagtggtacg gaaaaagctc actactgttt caccaaagtc tatttctgta
    21121 tgctcatcta ccacatggaa atccttaaat cctttggctg cttcattacg actgacattc
    21181 aattttgcta actcgatcgt caactctgtc ccgaatactg gtacagaaat ttttgacaac
    21241 aagtaaggaa gtgccccgat cgcatctgca tgtccatgtg ttaaaaagac accagctaca
    21301 cgatcaatgt tttcttctaa atacgtaaaa tcagggatta cgacgtcgat ccccaacaac
    21361 tcattttcag gatattttag cccacaatct aaaataaaaa tatcctcgcc cacttctgcg
    21421 acatacatgt tttttccatt ttcacgaacg ccgcctaatg ggataatttt aatggtactc
    21481 acaaacttca cctcttctta tgacatccaa gtttaaagat gtcgattttc ttctttatct
    21541 aaatatatga gccattccaa tgaagcatat gcaaaatcac tatttctcaa cttatggaga
    21601 atcagcagga aaattaccag cacatccgcg ttctgacagt tggatgggca ttttattttg
    21661 gatatacttc attaacaaaa tggatctttt gcgaaacgct atagccagaa tacttatata
    21721 agtattcagc tgttccccat tattatacaa caaataaact aaatcgctag tatcagcaat
    21781 tgttagaaca actatgcacg taaaaatccc tctgtagcat ctaggatttc ttttgctatt
    21841 cctctttcat ttcttcttcg tcggtttgaa acatactttt ttcattttac cacatttttt
    21901 gcaataacta tagtgacttt ttatatatac agaacctcat gttgatggtg tgcaaacagc
    21961 ataagattta agctagcttt aagataggtt gtctattatc aaatgcatct actaggaggg
    22021 atgcaaaata tgaattttat gaaacgtgca tggaaaagta caaaagtaaa atggggacgt
    22081 tcattgttat tgtttgccgt gttcacagcc attcttattt ttgtgctagc tggcctcacg
    22141 attcgaagcg ctgcagaaga agcagcgaat caagcacaaa aagaagttgg agcaacagtg
    22201 actttaagtg ccaatcgaga agcttcattt aaaaaacagg aacaaacttc ttcaagcggt
    22261 tctaccactg aagaacgtcc agatccaggc agtttttctc ttacaccagt ttccttaacg
    22321 gacgcggaaa aaatagctga attagataat gtaaagagtt attcctttga aagttccgcc
    22381 tcagctatgg caaaagatgg aataactgcc atcactagtt ctgattcaag tgaagacagt
    22441 tcagaaacta ctgatgcagg cggtgaacct gctggaggaa tgggcgggaa tccgcaaatg
    22501 actcaagcag actttcaaat cagtggtgtg tcagcaactg cacaaaatag taatttttct
    22561 gatggaacag caaaaatcac ggatggtgaa ggaatagatg acagcgacaa aggcacaaac
    22621 aatgtagtca ttgattctac tctagcagaa acaaatgacc tggcagtcgg agatactttc
    22681 acaatcacga gtactgaaga tgaagatacc acttatgaaa tgacgatcaa aggaatttat
    22741 gaatccagcg aaacaagcgg cagtatgggt atgaaattca acttcatgaa tccgtcgaat
    22801 acactttaca cttcttacac ttttgcaaat gaactaaatg ggacatctga cgataacacg
    22861 atagattctg ctatttacac tctatctgat ccagataaaa tggatgagtt tctcaaagaa
    22921 gctgagaagc tgatcgacac agatacattt agcttacaat caaatgattc catgtatcag
    22981 tcaatgttgg aacctttaaa caacgtggca agtttttcta aaaatgttgt tttactagtg
    23041 gctgtagccg gtattatcat acttactctg atcattatga ttaccattcg ggagcgtcgg
    23101 catgagttag gcgtcttgct atctttagga gaatccagaa gtaaaatcat tctccaattg
    23161 tttaccgaag tggctatttg tatgattctt gcattaggag ttgcttcgct tagcggaaac
    23221 gtcgtcgcga atgccgtggg acagcagcta cttgaccaac agacagaaac cacgacacaa
    23281 aaccagcaag ctcctggcgg tggacaaatg ccaggtaaca gacaagaaca aggcggtcaa
    23341 cgcgatggtc aacaaggaac cacgaataat ccatttgctg tttctgatca agtcagcgaa
    23401 ttggaaatca atgtcaaacc tgcacaattg ggactattag ccggagtagc atttggaatc
    23461 agtctgtttt ctgtcttttt agcttctata gggatattaa gattaaaccc aagaaaaatc
    23521 ctattaaact aaaggaggaa tttgatgtta cggacagaac aattgggatt ttggtatcaa
    23581 gatccgcaag atgcgcttta taaagatgta gaactgaatt tcgaagctgg aaaaatgtac
    23641 gctattttag gagcaagtgg ttctggaaaa acgacttttc tttcgcttat ttctggacta
    23701 gatgcaccaa aagaaggaaa ggtatactat caagatcgtt cactgaacaa aatcgggtta
    23761 agaagttatc gtaaaaatga tgtctcgatt attttccaag cttacaactt gcttccttat
    23821 ttatcggctc tagacaacgt catagcagca atggaaatca ctaagaccaa gcaagagaat
    23881 tatcgacaag ttgctttaga tcgactggct cgtgtaggaa ttgatgaaat attagcaaaa
    23941 aaacaggtga ccaaactttc gggaggtcag cagcagcgag tcgctatcgt tcgtgctatc
    24001 tgctgtgaac atcaactaat cgtagcggat gagcctactg gaaatctaga tgaaacaact
    24061 tcacaagata tcgtcaaact tttccaagaa atcgcccatg aacaaaatcg ctgtattatc
    24121 atcgtgactc atgagattga agtagcaaaa agctgtgatg aagtctttga actaaaaaat
    24181 aaaaaatttc aaaaaatcga agtttaaaaa acaggataat gatcaaataa aaaatccgaa
    24241 ccatatctgt ctcgcagata tggttcggat tttttatttg cttgattcaa ttagtaataa
    24301 gtggttttta ttttttttcg tcttttcgag aaaacttgtc tttgacttcg tcgcctaaat
    24361 ttcgagcttt ttctttgatg tcagcaccta gtgattgtgc tttcccttct gtttctttgc
    24421 tcttgtcgtc tgttgctttg ccgtatgctt cttttccttc accgactact ttatctttca
    24481 tatctttaga acgtcctttt aaatctgcca ttgtgcttcc tcctcttagt ttgtttattg
    24541 atctaccttc agggtaacat caacaacaga tacagacaaa cagaacgctc gttttctaaa
    24601 aaagatagtt agagctgttg attaaaaact tttttcgaaa gtcccccata ctttgacttc
    24661 tttttcttgg accattattt ctcctttagc gataactgta tcaatatcaa gcgtttcttt
    24721 atccaagata cacagatctg catcagcacc aatttcgatt tttccttttt tatccaattt
    24781 caatatacgt gctggattac tggttatagc tcgtaaggcg atttccaaag ggatactttc
    24841 tttttgcaca gcttctttga tccctactag caatgccttg gcacttccta ctcctaaccc
    24901 taggaaatga ttgttttcat caaaataagg caagcttcct tgtccatctg aggtcatcgt
    24961 gaaattatct aaagaaacat cttcctcgat caatcgtttc agtgctttgc tgaagcgaac
    25021 ttcaccatct gtcttctccc aaaaatcagg atcttcgcta gcagtgaagt caatcgtgcc
    25081 accacgtttg gcaaatgcca cgcaagcttc aaataattct ggtgtgcgat ttgcatgagt
    25141 aggcaaaaat tgtgtgatcg gaatttctgt ttcttctacg attcgtgtca atagctctag
    25201 ctttcttttt cctccgccta agtggacatt gatgatccca gcttttccgg ataacatgcc
    25261 tcctacacga gcatctgcta ctgcacgagc gaactcttca aaacttggct gagaagaacg
    25321 gtgatcggat actgcgattt ctccgattcc gataatttta tcaattgtca aaaagtcttt
    25381 gatgatagaa tctgttactg tttttaccgg caatcggtac gaaccttcgt atacataagt
    25441 ggagattcct tctgcttcca gacctttcgc tttgctgatc aacgcagtca tatctctacc
    25501 ttctccgtct gttcccaaac aaccgacaac tgtcgttaca ccagctgtag tcaattggct
    25561 caatgtgact tctggcgttc gattttgata accgccttct cctcctccac ctaaaatatg
    25621 gaaatgacag tcaatgaaac ccggggttag tacttttccc tcacctgaaa gttcttctac
    25681 ttccactcct tcgtagccgc cttctagctg ttcttctaca gccaaaatct tcccaccggc
    25741 aatcagtaca tccatttttc ctaagtaatc tggtgcaaac acttcaattt gtcgaatgat
    25801 tttcatacat ttttcctctt tctttaagta taactgatga taggatctta aaaaataatc
    25861 caaacgatag gatctttact cctttgattt taccgtaaaa tgacggttct tggagtcctt
    25921 atctactgtt tggattactt ttttatttac aggtctccag tactactgca gacctattgc
    25981 aatggcaatc atgatgaaaa tagttcctaa aacaaacagg aatccttgca tcttgatttg
    26041 gaatcttgcc cattttgccc attcgatccg tgcaacacct agcacaccaa tcaagctggc
    26101 tgatacgggt gtaaaagcgt ccataaaacc tgaaccaagc tgataagcaa gaactgctac
    26161 ttgtctggat acacctacta aatctgccag tggagccatg attggcatag tcaaagctgc
    26221 ttgtcctgaa tttgatgtaa caaccaagtt gaacaaactt tggaagatgt acatcgccca
    26281 agcaccaatg acagctggaa caccggtcaa cgccgtcccg atcccatgca aaatcgtatt
    26341 caaagctgaa ggaacagatg catcagatcc tcccaagaca agaagaatcc ctttggccat
    26401 acctactacg attgctgtac cagctaaatc agctgcacca gattggaaag aagaagcgat
    26461 gtcattgact ttcattccat ctaatttgaa aagaacagca atgattccag caactaatcc
    26521 catcacaaag aattgagaag cgatttctgg aatgtaatag cctttttgag tcacgcccca
    26581 aacgatccag acaaggacaa ctaacatctc taataagatc aatttatgcc caagcatgaa
    26641 ttttttattt tcatcagatg tctttttgat atgagaacgg aaataatcat ctgatttgta
    26701 agtcaacgaa ctttctggtt ttttacggat tttttctgca tatatcatca tataaccagc
    26761 ggctaatgca gtaacaacta cccacataat caatcggaat gtcgcaccag acaacacggg
    26821 gattccagca atcccttgag ccaccgctac gctaaacggg ctcatccaag aggtcgcatt
    26881 tccgacttga gatgcgacat aagtcacggt tactgctacg atcgagtcat atcctaacgc
    26941 aatgacaaat ggaaccatca ccattgagaa tggaattacc tcttctgcca tcccgaatgt
    27001 agcgccaccg aatgaaaaag caaagaataa tagcggtaaa gctaaacgct ccagtccctt
    27061 agttttactg ataaacgcat agatacctgc gtcgatcgct ccggtacgca taataatacc
    27121 aaaagcacct ccaacaacaa ggatcaatgc agcgatccct acagcagatc catacttatc
    27181 tccagatact agtccttcaa aaacaaaatt taagaaacca aagccgccaa aatcatctgt
    27241 tccccatatt ttggctgttt tatgcaattt tttagaagta tcatagatat tttcgccgta
    27301 ctgatcgtaa agcacatcat ctgtcaagga gatttcatct agtttttcct gcgtaagatt
    27361 tttttctccg tcagcaatga ctttttccaa tcctttttcc ggaacatcca gtttttcacg
    27421 ttctgctgga tgatcttgca gttcttctaa ttgatcaaac acatacgact tatccagctc
    27481 gtaagcataa cggaaggaat cttgacgtaa aacagtacga gtacttgttt cgccatttgc
    27541 atctttgtat tcaatatctt gcgtgctgaa ctttcctgct ggaacgacaa aagtcagtat
    27601 ccatgcaaag atgactacac caaaaataat gacatacgta tggggtgtcg ataatttagt
    27661 gaagtcgcgt ttcggttttc ctgaagaatt tgttttcttc aaatttattt cttcctttct
    27721 tttttcagta attttgatga ataaaaaatg gataatcaaa tgaattatac agtaaaaatg
    27781 acatttccac atgaataatt tattagaaaa tcactttttt ctattttgtt ttttttctcc
    27841 tatgtatgat tatttttttc tctttccatt tatattaagt attttatttc tctgtttttc
    27901 cttaaaattc tttttttatt tttagattag agaaaatctc gtcctgtttt tttcatttta
    27961 caatcattga aaaattgaat ttcattgcaa aaaaaataat aaacacttat aaatatccat
    28021 tatctatcct tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca
    28081 attttgttat atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt
    28141 tgtaaagagt tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc
    28201 atatggattc tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc
    28261 ttataactca tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa
    28321 caagcttaaa atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg
    28381 ttaatcctca ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt
    28441 atttttttaa ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta
    28501 gctgttttgc tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg
    28561 ttccgcagat cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt
    28621 ggttgtccaa aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt
    28681 tgatacttcg tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga
    28741 tccactgagc tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa
    28801 tgtcttctga tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt
    28861 catagacacc tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat
    28921 cccaaaattc aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac
    28981 cttgattaat ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc
    29041 ttaatttctc ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc
    29101 gcctagctat acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa
    29161 acagtagtta gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga
    29221 acgaatacgc ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat
    29281 ctacataggg gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc
    29341 attgactatg gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc
    29401 ttagcgccaa ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat
    29461 tgtaagtaat ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt
    29521 atatatatat aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt
    29581 taaaaaagat tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct
    29641 catctcaata gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg
    29701 tcttcttctc gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg
    29761 tctacctttt ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata
    29821 cagtacagca aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac
    29881 catcaaggta gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt
    29941 ttttctgacc atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat
    30001 ccaattgctt ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac
    30061 taattttccc atagtgaaca cacacctttt tttataatta aaaaattcgc ttttttattc
    30121 tactatgaat cttctggctt ggctttcatt tctcttatgt aatcattttc ttttatacaa
    30181 cttaaaaaaa aattaaacca ttagcataat attttctgaa tctattcacg aaagtctttt
    30241 tctatctttc gttttccacg actcaactga tctagagtca aaaaactaaa acgacagttg
    30301 cgcaaatgac gcacaagctg ccgctttaat aacaaatagc ctttcattct cagcaggcca
    30361 cttatttcca gttaactttc tgttcttagc tttttactca actggataat atatgcactt
    30421 aatgaagtcc caatttccga atgttgaagt ccatattcaa tatttgtctt catatatcct
    30481 aacttatcgc ctacgtcgta tctttttcct ttaaactctc tcgcatagac cgcctctgtc
    30541 tggttaagag tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc tggttcttga
    30601 gtttctaata tctcaaatat tttaggagtc agtagataac gtccgataat ggctaaatta
    30661 cttggtgctt ttcccatttc aggtttttct acaaaccctt ttactctgca acgacccttt
    30721 tcgctatctg ttttaggatc aataattccg tatttagatg tttcattttc tggtacacgc
    30781 atgacggcaa cagcggaagc ttgtgtttct tcataatcca tcatcaattg ttgagtcaat
    30841 gggatatcat ctttcatcaa atcatccccc agcatgacga caaacggttc atttcctata
    30901 aatgcttttg cttgaagcac ggcatccccc agtcctcttg gatgagattg acggatgaag
    30961 tgaagattga cttgagtcgt ttcttcaact aaagcgagta attctgtttt ttccttttcc
    31021 cgcaaattgt tttccaattc gatatttgca tcaaagtgat cttcaatggg tcttttttct
    31081 tttcccgtta cgatcaagat gtcttcgatt cccgaatcca atgcttcttc cacaataaac
    31141 tggatcgttg gtttatccac gattggaagc atttcttttg ccatggcttt tgttgccggc
    31201 aaaaaacgtg ttcctagccc agcggcagga atgactgctt ttttgacctt cactt
//
LOCUS       DACDYM010000029        29572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDYM010000029 DACDYM010000000
VERSION     DACDYM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.029"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="IWM59_001305"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="IWM59_001305"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9979350.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IWM59_001306"
     CDS             complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IWM59_001306"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9979351.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IWM59_001307"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IWM59_001307"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9979352.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="IWM59_001308"
     CDS             complement(3376..4683)
                     /locus_tag="IWM59_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9979353.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="IWM59_001309"
     CDS             complement(4686..5267)
                     /locus_tag="IWM59_001309"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9979354.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="IWM59_001310"
     CDS             complement(5839..7131)
                     /locus_tag="IWM59_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9979355.1"
                     /translation="MKKKYVQVGLGGRARFFYETIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="IWM59_001311"
     CDS             complement(7128..7751)
                     /locus_tag="IWM59_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979356.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="IWM59_001312"
     CDS             complement(7754..8590)
                     /locus_tag="IWM59_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9979357.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="IWM59_001313"
     CDS             complement(8607..9494)
                     /locus_tag="IWM59_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9979358.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="IWM59_001314"
     CDS             complement(9510..10793)
                     /locus_tag="IWM59_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9979359.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="IWM59_001315"
     CDS             complement(11476..12159)
                     /locus_tag="IWM59_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9979360.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="IWM59_001316"
     CDS             complement(12177..12794)
                     /locus_tag="IWM59_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9979361.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="IWM59_001317"
     CDS             complement(12804..13628)
                     /locus_tag="IWM59_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9979362.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="IWM59_001318"
     CDS             complement(13621..14181)
                     /locus_tag="IWM59_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979363.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="IWM59_001319"
     CDS             14355..15311
                     /locus_tag="IWM59_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9979364.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="IWM59_001320"
     CDS             complement(15616..16572)
                     /locus_tag="IWM59_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9979365.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16676..17278
                     /locus_tag="IWM59_001321"
     CDS             16676..17278
                     /locus_tag="IWM59_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9979366.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17366..18865)
                     /locus_tag="IWM59_001322"
     CDS             complement(17366..18865)
                     /locus_tag="IWM59_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9979367.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18881..19405)
                     /locus_tag="IWM59_001323"
     CDS             complement(18881..19405)
                     /locus_tag="IWM59_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979368.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19703..20452
                     /locus_tag="IWM59_001324"
     CDS             19703..20452
                     /locus_tag="IWM59_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9979369.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20443..21228
                     /locus_tag="IWM59_001325"
     CDS             20443..21228
                     /locus_tag="IWM59_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9979370.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21197..21913
                     /locus_tag="IWM59_001326"
     CDS             21197..21913
                     /locus_tag="IWM59_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9979371.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22015..23034)
                     /locus_tag="IWM59_001327"
     CDS             complement(22015..23034)
                     /locus_tag="IWM59_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9979372.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23053..24111)
                     /locus_tag="IWM59_001328"
     CDS             complement(23053..24111)
                     /locus_tag="IWM59_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9979373.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24332..25480)
                     /gene="nagA"
                     /locus_tag="IWM59_001329"
     CDS             complement(24332..25480)
                     /gene="nagA"
                     /locus_tag="IWM59_001329"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9979374.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25556..26392)
                     /locus_tag="IWM59_001330"
     CDS             complement(25556..26392)
                     /locus_tag="IWM59_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9979375.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26576..27793)
                     /locus_tag="IWM59_001331"
     CDS             complement(26576..27793)
                     /locus_tag="IWM59_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9979376.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            28176..28259
                     /locus_tag="IWM59_001332"
     tRNA            28176..28259
                     /locus_tag="IWM59_001332"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28210..28212,aa:Leu,seq:gag)
     gene            complement(28499..29497)
                     /locus_tag="IWM59_001333"
     CDS             complement(28499..29497)
                     /locus_tag="IWM59_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979377.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         8844 a         6303 c         5063 g         9362 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgtctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc
    15421 ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
    16621 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
    16681 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
    16741 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
    16801 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
    16861 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
    16921 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
    16981 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
    17041 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
    17101 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
    17161 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
    17221 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
    17281 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
    17341 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
    17401 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
    17461 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
    17521 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
    17581 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
    17641 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
    17701 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
    17761 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
    17821 gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
    17881 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
    17941 tgatttcttt ttcttccagt tcgatttgtt tttgacgttc tacgatttta atctgcattt
    18001 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
    18061 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
    18121 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
    18181 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
    18241 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
    18301 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
    18361 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
    18421 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
    18481 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
    18541 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttccgtatac acttcagggg
    18601 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
    18661 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
    18721 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
    18781 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
    18841 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
    18901 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
    18961 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
    19021 cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
    19081 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
    19141 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
    19201 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
    19261 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
    19321 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
    19381 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
    19441 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
    19501 gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tttcacaata
    19561 aattctttcg aggtagccta tgtttccttt ttttaagaaa aaaccattaa agctccagaa
    19621 cacgatcatc cttgtcgttt gtttctccac attcgtttct ttgattgttt ctgccatatt
    19681 gatcaggaat tttattcttt cgatgaaccg atctagtgtg tcagataaaa tctctagcat
    19741 tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag ctgcagactg gtgacccgac
    19801 acatgcgatt caaggaataa caaatgaaat catgattgca tcgaatttag attttatcgt
    19861 tgtcatggac cctgaaggca ttcgatattc tcacgcagac ccaagttaca tcggtcttcc
    19921 tttctcaagt catgaagatg tgaaaaaagt gattggagga caagaatttt tctctgaaaa
    19981 agaaggaatg ttagggttcg gcttccgcta tttcgtccct atttattatg atcaagagat
    20041 tattggtgca gtttgtgttg gcttaacaaa caacacgatt gacaatcaag tcaaagatgg
    20101 acaaaaaatg attgtttttt cattgattat tggacttagc gtaggcatca taggcgccgt
    20161 ttttcttgcc caaaaaatga aaaaagtact gcttggactt gaaccacaag aaatagcaga
    20221 acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct tagccatctc
    20281 ttctgataaa actatcctct tgatcaatac acctgcaaaa aacattttct accaaacaga
    20341 gcagctattg gatttcgaag taggaaaaaa atcagtgata atctatatga cgttttgttt
    20401 aaagagacga ttgagcatca aacgaccgtt cggactcaat ccatgtattt aaatgggata
    20461 gaaactattt ctagcgttac acctcttttt agcaatgacc gttttttagg agcagtcgca
    20521 actttacgaa atcgatcaga agtaatgaaa ttaatggagg agcttggggg gacgaaacaa
    20581 tatataaact ccatgagagc tcaaacccat gaattcatga ataaacttca tgtcatttct
    20641 ggtttgattg atttgaagaa gtatgaagaa gtcacccatt ttatccagca gctaaatcaa
    20701 aattataaaa aagagattgg ccaagtctca agtctgatca aagtacctgc aattgccggt
    20761 tttttattag gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc
    20821 gattctttta tccctaaaat ggagatggat gactccatcc attcactttt gcaagtgtta
    20881 gggaacttac ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag aaacggacag
    20941 gtccggattc atttaaacta tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat
    21001 gacggtgtcg ggatatcaga agaaatctca gaaaaaatat ttacgctagg tttttcgacg
    21061 aagggaatca atcgtgggtt tggattgaat ctcatccatt caatcgtcaa acgccacggt
    21121 ggatttttag aattttccaa acaaacaaat ggcggtacaa tcgtctatct agaattacca
    21181 aaaaaggaag atcaaaatga tgacaaatgt cctgataatc gaagatgatc caatggttgc
    21241 agaattgaat cgtacttttg tggaaagcat ccctaatatg aacgtaatcg aaagtttcag
    21301 aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc tgctgcttga
    21361 tgtatacatg cctggcgaaa gtggtatcca ttttttatct tggcttagac agcaaggaaa
    21421 tgatatccca gtgattttga tcactgctgc agatgatatc gaaaccataa agaatgtgat
    21481 ccgctacggt gccttagatt atttgatcaa accgttcact ttgcaacgtt ttcagctggc
    21541 gatcgaaaag tatcttgctt tctctcagac aatccaaacg actgaaaaag ccgatcagaa
    21601 attgatcgat caagtattca accacaccac tcccattcac gaaataccat cattcatcga
    21661 tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata agtgcattta ttctaaaaga
    21721 agatactatt ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac gtatatccgt
    21781 caagaaatat ttgaattttt tagtagaaca agatatattg aacgaaagaa tcaaatattt
    21841 ggaaatcggc cggccgctaa cctgttatga attgacaaag aatgggcgga aaaagctcac
    21901 agattactgt tgagtcatcg aattatccca tacaaaatag tccaagtata cagaagtaac
    21961 aactgtttac ttggactatt tttaaacatt ttattgacga atatccatgt tttcttatct
    22021 taattgacgg gctttaggaa gagatacctc atttttcgtt tttccagttt ctattatttc
    22081 acgaaagttc tcgaaacttg tcgaaatcat atttgataat gcctcatctg tatttgaacc
    22141 tacatgcggg gttacaagaa ctctagggta catttctact aattgctgga tagcagggtc
    22201 tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac
    22261 atcggttgca aaaccagcta aatgattgct tttcaaagct cttaaaatag cttcattatc
    22321 ttgaagctct ccacgggctg tattaatcaa gatgctacct tttttcattt gagctaaaaa
    22381 tccttcattg atcatttgat cgttttcacc tgggatatga ggtacatgaa tactgacaat
    22441 atcgcttttg gctaataact cttcaagcga gtggaacgtc aatacttttt tagcagcttc
    22501 tgtctgatga atatcgtagc cgatcacttt ggctcctagt cccttaaaca aaacagcttc
    22561 cgtcaaccct atcctgcctg ttcctatgat ccctactgtg caatttctga tttcacgact
    22621 aaacatttgc tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt
    22681 atttctcaaa agcatcatgg caaaagtcaa agataactct gctatcgcat ttggcgaata
    22741 cgatggtaca cgggctactg tcataccaaa atcagctgcc gcttgtagat cgatatgatt
    22801 gactcccaca gtccgtgtaa aaacataacg aatcccatat tcctgcattt ttgcaagatt
    22861 ggtctcatct gctatacaat ttcccctcaa caatacagca tcatggtcct ttgctgacac
    22921 gatattgtca tgtgtcaaca gttcttcata aagactgaga tcataatggt aaatattcaa
    22981 tttttcaaaa aaatctatct cgttcggacg aacaccgtaa catgcgattc tcatactttc
    23041 ttcctccatt tcttatgaaa ataaacctat gccatgaatc atttgtaata caacgatcca
    23101 tacaggtgtc agtaccacag caaaaatagt tgacagtaag gatgcattgg aggctaaaag
    23161 tgcctcccta tcaaaactga tagcataggc tgctgccact gttgcagctg gtgtagccat
    23221 catgataacg attgttgcca gtgcttcaaa attgactggt agaatattcg ttgccgtcaa
    23281 aactgctaga atcacgatat tcaatgcagg tacaaatacc actttgacta gactgtaata
    23341 ccatgaagtt ttttcagatg cagctttttt gaagcttact tcacctaatg ttgcgccgat
    23401 tgctagccac gccaatggtg aacttaaacc agctaaataa tccatcggct gaaatagcca
    23461 cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt atctctcctt ctgcacctgt
    23521 aactgacact tgtggcagat acccttggaa cagccaaatg aaaaggccgg caaaagtagc
    23581 gataatgatt ggattgacga acatcgcttt gatattttta gcttccatct tcaagccact
    23641 catttttatg tatccataag aatacaaaaa gattcggtag ccaatattga aaattgagga
    23701 atacatcacg ccgacaggac cataaatcgc actgacgata ggcgttccga aaaaggtcgt
    23761 agaaccaaag atcgtcagga cacgcaacgt tgtttgctta tcccctttat agcttaagaa
    23821 aaaaggtttt gaaactagaa taagaataat ataaataact aatccccaaa tcaagacatt
    23881 gattccttgc atcagtaatt ctgtattgat atcttgcatg aaagctcgaa atgccagtgc
    23941 tggcaaagcc actgttaaga cgactttcga tagaattttt ccaacagatt cagaaaaaat
    24001 cccctttttt ctacaaacaa accctaataa tataataaaa actgttgaag tgattgcact
    24061 gacaatcgac atatctgtca aagtcgatcg aaaaatgtca aataagttca tctttctcct
    24121 cctaattatg ttcatttatt cacaataaaa aaagctttta ttttgctaat acgtattaac
    24181 atttttaatt ttatctttac attgttactt attgaacaat aggaaattaa agaaactttt
    24241 gaaacttatg taaagtgatt tttactagaa cacaaaaaaa accttaaaac caaacttaag
    24301 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
    24361 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
    24421 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
    24481 tcatgactgc ttgttctggt gttgctaatc cccagtctac aacattttta attgcttctt
    24541 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
    24601 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
    24661 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
    24721 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
    24781 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
    24841 caaatacact tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta gcattactgt
    24901 gtcctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
    24961 gacgttccgg tgctaaagca atttttttga ttaaaccacc tgaagcttct tgccattcat
    25021 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
    25081 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
    25141 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
    25201 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
    25261 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
    25321 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
    25381 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
    25441 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
    25501 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
    25561 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
    25621 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
    25681 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
    25741 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
    25801 ttgatcgttc atcagctgat tgatgcagca gttggatgct tcgactcaaa tagtagcgtg
    25861 cttcagccgt ttttcctgcc acaccatata tttttccgat acaactaatc agttgtattt
    25921 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
    25981 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
    26041 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
    26101 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
    26161 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
    26221 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
    26281 gacttaatac actttgtgaa caaatatcat gcgaaagcat tttttgcgtc attttttgtt
    26341 cttttcgaat ctctttaatg atcgttccaa aggactcgat taacgtttcc ataagtatcc
    26401 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
    26461 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
    26521 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
    26581 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
    26641 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
    26701 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
    26761 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
    26821 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
    26881 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
    26941 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
    27001 tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
    27061 ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca ggcgctaatg
    27121 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
    27181 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
    27241 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
    27301 tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
    27361 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
    27421 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
    27481 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
    27541 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
    27601 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
    27661 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
    27721 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
    27781 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
    27841 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
    27901 aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc atgatagtca
    27961 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
    28021 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
    28081 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
    28141 gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa ttggcagaca
    28201 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
    28261 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
    28321 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
    28381 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
    28441 taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt tttgtatatt
    28501 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
    28561 taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc attagtacta
    28621 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
    28681 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
    28741 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
    28801 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
    28861 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
    28921 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
    28981 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
    29041 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
    29101 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
    29161 atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta
    29221 attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa
    29281 tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat
    29341 tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt atttcttctc
    29401 ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact ggccactgat
    29461 agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt attaaacagt
    29521 atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DACDYM010000030        28635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDYM010000030 DACDYM010000000
VERSION     DACDYM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.030"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IWM59_001334"
     CDS             7..624
                     /locus_tag="IWM59_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979378.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="IWM59_001335"
     CDS             1644..2813
                     /locus_tag="IWM59_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9979379.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="IWM59_001336"
     CDS             3286..3840
                     /locus_tag="IWM59_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9979380.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="IWM59_001337"
     CDS             complement(4013..4486)
                     /locus_tag="IWM59_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9979381.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="IWM59_001338"
     CDS             4498..5034
                     /locus_tag="IWM59_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9979382.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="IWM59_001339"
     CDS             5074..5445
                     /locus_tag="IWM59_001339"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9979383.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="IWM59_001340"
     CDS             5729..7000
                     /locus_tag="IWM59_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP9979384.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="IWM59_001341"
     CDS             7245..8270
                     /locus_tag="IWM59_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9979385.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8564..9673
                     /locus_tag="IWM59_001342"
     CDS             8564..9673
                     /locus_tag="IWM59_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9979386.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9756..10652
                     /locus_tag="IWM59_001343"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="IWM59_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10668..11492
                     /locus_tag="IWM59_001344"
     CDS             10668..11492
                     /locus_tag="IWM59_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9979387.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="IWM59_001345"
     CDS             11524..12834
                     /locus_tag="IWM59_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP9979388.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="IWM59_001346"
     CDS             12982..14241
                     /locus_tag="IWM59_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9979389.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="IWM59_001347"
     CDS             14251..15123
                     /locus_tag="IWM59_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9979390.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="IWM59_001348"
     CDS             15135..15965
                     /locus_tag="IWM59_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9979391.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="IWM59_001349"
     CDS             16005..18188
                     /locus_tag="IWM59_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9979392.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDKTVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="IWM59_001350"
     CDS             18249..19469
                     /locus_tag="IWM59_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP9979393.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="IWM59_001351"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="IWM59_001351"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP9979394.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="IWM59_001352"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="IWM59_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="IWM59_001353"
     CDS             22431..22640
                     /locus_tag="IWM59_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9979395.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="IWM59_001354"
     CDS             22633..22782
                     /locus_tag="IWM59_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP9979396.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22817..23601
                     /locus_tag="IWM59_001355"
                     /pseudo
     CDS             22817..23601
                     /locus_tag="IWM59_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="IWM59_001356"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="IWM59_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9979397.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="IWM59_001357"
     CDS             25304..25537
                     /locus_tag="IWM59_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9979398.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="IWM59_001358"
     CDS             25534..25941
                     /locus_tag="IWM59_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9979399.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="IWM59_001359"
     CDS             complement(27078..27596)
                     /locus_tag="IWM59_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9979400.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         9844 a         4313 c         5243 g         9235 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgataaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcgttact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc actta
//
LOCUS       DACDYM010000031        28107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDYM010000031 DACDYM010000000
VERSION     DACDYM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.031"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..686)
                     /locus_tag="IWM59_001360"
     CDS             complement(105..686)
                     /locus_tag="IWM59_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9979401.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(869..1495)
                     /locus_tag="IWM59_001361"
     CDS             complement(869..1495)
                     /locus_tag="IWM59_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9979402.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1517..2848)
                     /locus_tag="IWM59_001362"
     CDS             complement(1517..2848)
                     /locus_tag="IWM59_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9979403.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3003..3404)
                     /locus_tag="IWM59_001363"
     CDS             complement(3003..3404)
                     /locus_tag="IWM59_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9979404.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3490..3696)
                     /locus_tag="IWM59_001364"
     CDS             complement(3490..3696)
                     /locus_tag="IWM59_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979405.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3791..5293)
                     /locus_tag="IWM59_001365"
     CDS             complement(3791..5293)
                     /locus_tag="IWM59_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9979406.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5311..5766)
                     /gene="argR"
                     /locus_tag="IWM59_001366"
     CDS             complement(5311..5766)
                     /gene="argR"
                     /locus_tag="IWM59_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9979407.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5864..6481)
                     /locus_tag="IWM59_001367"
     CDS             complement(5864..6481)
                     /locus_tag="IWM59_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979408.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6702..8075
                     /locus_tag="IWM59_001368"
     CDS             6702..8075
                     /locus_tag="IWM59_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9979409.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8150..9262
                     /locus_tag="IWM59_001369"
     CDS             8150..9262
                     /locus_tag="IWM59_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9979410.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9334..10971)
                     /locus_tag="IWM59_001370"
     CDS             complement(9334..10971)
                     /locus_tag="IWM59_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9979411.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11139..11747)
                     /locus_tag="IWM59_001371"
     CDS             complement(11139..11747)
                     /locus_tag="IWM59_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9979412.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12131..13282)
                     /gene="queG"
                     /locus_tag="IWM59_001372"
     CDS             complement(12131..13282)
                     /gene="queG"
                     /locus_tag="IWM59_001372"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9979413.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13301..14053)
                     /locus_tag="IWM59_001373"
     CDS             complement(13301..14053)
                     /locus_tag="IWM59_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979414.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14194..15144)
                     /locus_tag="IWM59_001374"
     CDS             complement(14194..15144)
                     /locus_tag="IWM59_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9979415.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15145..15984)
                     /locus_tag="IWM59_001375"
     CDS             complement(15145..15984)
                     /locus_tag="IWM59_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9979416.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15981..16727)
                     /locus_tag="IWM59_001376"
     CDS             complement(15981..16727)
                     /locus_tag="IWM59_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9979417.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16965..18335
                     /locus_tag="IWM59_001377"
     CDS             16965..18335
                     /locus_tag="IWM59_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9979418.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18390..19286)
                     /locus_tag="IWM59_001378"
     CDS             complement(18390..19286)
                     /locus_tag="IWM59_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9979419.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19567..20244)
                     /locus_tag="IWM59_001379"
     CDS             complement(19567..20244)
                     /locus_tag="IWM59_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9979420.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20335..21240)
                     /locus_tag="IWM59_001380"
     CDS             complement(20335..21240)
                     /locus_tag="IWM59_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9979421.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21415..22869
                     /locus_tag="IWM59_001381"
     CDS             21415..22869
                     /locus_tag="IWM59_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9979422.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22885..23916
                     /locus_tag="IWM59_001382"
     CDS             22885..23916
                     /locus_tag="IWM59_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9979423.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23923..25110
                     /locus_tag="IWM59_001383"
     CDS             23923..25110
                     /locus_tag="IWM59_001383"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9979424.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25266..26648
                     /locus_tag="IWM59_001384"
     CDS             25266..26648
                     /locus_tag="IWM59_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9979425.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26620..27006
                     /locus_tag="IWM59_001385"
     CDS             26620..27006
                     /locus_tag="IWM59_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979426.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27206..27874)
                     /locus_tag="IWM59_001386"
     CDS             complement(27206..27874)
                     /locus_tag="IWM59_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9979427.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7830 a         5748 c         5159 g         9370 t
ORIGIN      
        1 ttcttcgaaa aataagccga aatttcacaa aaatttgaga agcaattttc gtaaattcct
       61 tcttatttct tgaagctgac cacttctgtc acaacctcta attattaaag aaccttttgc
      121 gaagttaaca atctttcttt gatcaattga aaattctgcc taagtagtga tagatcctgt
      181 gtgactttca acaataagat acttccctgg atttcagcaa tcaacgcttt ggcttgacgg
      241 tctgcttctt catcggacag tttttcttca gaaagtgcca ggatattttg tttcgtatac
      301 ttgacccatt gattgactaa ttctagtagc aacagacggt attcttcatc cactgtactc
      361 aactcaataa tcaaattacc tacaggacag ccatgataag tgatctgtgt ctcatgatca
      421 gcgaaaatcc agtccaacat ttcttctaat gcttcttctg gctgttttcc agcatttaag
      481 atgtcttcga acaattctgt ctgccatacg tccatatgcg catggatcac cgctagacaa
      541 acttcttttt ttgaagaaaa gtaataataa aattgacctt ttcctgtatc agaagctgct
      601 agaatatctc ggattgacgt ttcatgatag ccttttttat aaataagatc tttggtcgct
      661 tccaagatct tttcctttgt tttcaatgat attctccttt ttcatttctc atagtcctat
      721 attatacagt aaattcatca ttttttcttc ttaatcgttt aaaaaagact gtgccaaatt
      781 atctgacaca gccctcacta taaaactgtt ctcatatagc tagatgttca ttgattcaag
      841 aactgcgagg acaaaagcaa ttcctatctt atgatagttg ctctaatact tcctctgttg
      901 tcatcacttg atctgcaata aagccaaagt tcgtcaacgc tgcatccatt gcttcttggc
      961 caggagcacc aattgcatct gtcgctacca caacattgaa tccactctct accaattcac
     1021 gcatatggga atcgctacac aagtttccgt tcattccacc aagaattact gtgtcgattc
     1081 cattttttct caattggagc gccaaatcat tgctttctgg tccaaagatt ttatgaggag
     1141 agctgatgat cgtattttca tccaaataag gtttcaattc ttcgataaaa tcggctcctg
     1201 aaccttcttc cactgattca tattgacttt ttctagcaaa catctgctgc tcaagcatca
     1261 tatgttctcc tgcaccgcca aattgccatt tatggtcatg tgggtaaaaa taatgaggag
     1321 aaataaagag tttgtatcct ttttcttttg cttgttttac catttcaatc atattttcga
     1381 tcgtattgta tttttctaag atatcttttg tcaaaccaaa gccaccgcct gctggtttca
     1441 agaactcgac ttgcgggtca gtgagaacta ctgctgtatt tttattattg aacatggtgg
     1501 attctccatt cgttatttat tttaatacat catttagagc ttcactcatt gtcggatgag
     1561 aatagatttg atctctcaat aacgtataag gaagttttcc attcatagct agcgaaatca
     1621 agttgatgac ttcatgagaa tcttccgcat agatacttgc ccctagaatc aagtcagttt
     1681 cagggtcgac taaaactttg aatacacctt taggatcttc taatacttgt gctttaggga
     1741 caccgcttgt catatacttg aataatttgt aattgacgcc ttgtgctttg gcttgttttt
     1801 ctgttaaacc tacattggat aaagtcggag tgataaatac actgtaaggc acagctttcc
     1861 ggttattcgt tgttcgttta ttctcgccct tcaactgatc cataatgatt cgataatcat
     1921 ctagagagat ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc caaatgttgt
     1981 ctgcatctgt acgaaggtat tcatccacag cgattgctcc gcgatctgtt agtttaacat
     2041 ctgtattttc taatccaagt gatgctgtac ttggttttct tccagttgca gccaaaactt
     2101 ttgacgcttg gattgttact tcttggccat cttctgtaaa ggtgacggca gctgttgatt
     2161 cttggtctgc tacttgatct acagatacac ctagatggaa ttcgattccc gcatttgtca
     2221 tgtcttctaa aatcatttgc gcgatatctt catcttcacg tggaagaaat tcagaatggg
     2281 catcaagaac gacaacttta gatccgtatt cattgaacat tgatgcaaat tccaagccaa
     2341 tatatccagc cccgatgatc actaattttt ccggtaattg ttcctgatcc atcgcttgcg
     2401 tagaatcaat caagtagcga cttgtattca atcctttgat tggtaaaatg attggttcag
     2461 aaccagtatt gatgaaaatc cgttcgcctt caacaatcac tttttcacca ttattcgtca
     2521 cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc catcactgtc ccagttgcct
     2581 catcgctgat catatgataa tttttggcgc gaagcatgcc cgtcaatttt tctttccgag
     2641 caacagcctc tgtaaagtcg attcctcttt ctccattgaa aatcaatgat ttagaaggaa
     2701 tacagccgat gttgatacat gttccgccat acatacgtgt tgacttttca acgactaaaa
     2761 ccgattcacc ttttgaagct aacgccttcg ctaaagtctt accgcccttt ccaaaaccga
     2821 tcacaatatt ttgatatttt tccatcattt ttccctcgct tcttttttac ctaccgattg
     2881 gtacaaaaat atgataaccg ataattgtct attctccaaa tcatctgctt tatttgtcaa
     2941 aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc tggttcgatt tcctttattc
     3001 ttctaaacaa tcgttacttc tacatcgata tttcctctag ttgctttgga gtaagggcag
     3061 aattcatgcg catcttctgc aattttttgg gcttcttctt cgctgatgcc atcgatatgc
     3121 acttctaata ctacaccgat ctgatagccg ccatcatcta aattataaag agagacacgt
     3181 gcttttactg tggactctgc tgtcattcct gcttctttga tcatatgttg taccgcccca
     3241 ttgaaacaag aagcataacc tgctgcaaat aattgttctg gatttgttcc ttctgcatgg
     3301 attcctggag gagtgatttt catatgcatc gagccatttg gtgcctcgac ttctccattg
     3361 cgcccgccat ggttgatcat tgctgtttcg tatgcttttt tcattgtact gctcctttcg
     3421 attgaccatt tgctacttgg ctataattat agtgtatgac aagtaaggcg ctttctacaa
     3481 aaattctgct tattcgaata ccagaatgcc gtcttctact atttttccaa aaaacaaaaa
     3541 aatactactc gtgaacaaca gcgtcatatt tgttccaaaa taaatagcaa tcttttctac
     3601 ataatctttt atatgattga ttttaagata catatatacc agaaagttga ccccataaac
     3661 caatactgtt aaaaaaataa ccatattcgt taccattttt tcctcctttt gctggtctcc
     3721 ctaccagcac tcgatttaac cataccactt tttttgccgt ccaagcaact agcctttcaa
     3781 aaagactggt ttattctgct gttaatgatt caagatcaga aaaatgtccg aataattttg
     3841 ctaattttac taacgccaac ttgttctttc cgaattcatc attttctatc tgtaatacta
     3901 gtaacggctc atgtaagctt aaacggatca aaaaccagcc gtttccgtat ggctcttcta
     3961 gatcatatcg aataccttct tcattttccg ggtcgaagtt catccctttt tcttcttcca
     4021 acagtcgctt catttcttct aatatcttgt ttccttgttc gcgtggttca tcgctgaaca
     4081 attttaatcg gatttcttgt acttctacgg gctgtttcaa ctctttgatt tgttcatcta
     4141 atgtgatacc ttttcttttt aattctggca aaagcatcag gatctttgcg atgacatatg
     4201 caccatcatc cagaaaatag ttttccttga atgcagcgtg tccgcttgtt tcgatggcta
     4261 gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt attgattaca tttcgatagc
     4321 cggaaatata acgaacttgg atgcctcctt tttcttcaat gaatctttgt agatgagaag
     4381 aagtagggga attcgtcacg attcttgttc ctggatgctc ttttaatacg attgtactta
     4441 atactgcgat caaattattt cgattgatca ctgttccaga agcactgaca actgcagaac
     4501 gatccacatc tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa acagcctgct
     4561 tgatactttc catcgcttct ttattatcag gatttgggat atgattagga aatcggccat
     4621 ctggttccaa aaattgggaa cctgttgaat ccgcacctaa gctattcaat actttttcag
     4681 caaagaatcc accagcacca tttccggcat caacaatgat tttccaacca tttagtggtt
     4741 tgctttctag tgttccaatc ccctttttaa tttttgtgac taaatcttgc gcataaggtg
     4801 tcaaaaggtc tgctttttct acgcttccag tttgttcgct ttttattttt tgctgctgtg
     4861 ataggataaa agcaatatct tctttttctg ctcctccttc tgaggtaaag aattttattc
     4921 cattataata atagggaaga tggctagcag tcagcataac cgcagcatcg cacgagaact
     4981 gttcaaattg agtggacata aacatagcag gcgtagtagc taattcgaaa tcgatgacat
     5041 gtatcccttg ttctttcaga ctttggatca acgcctcttt cagtgctggt ccgcttatcc
     5101 tactgtcatg accaatccca atcgtccatt ttttatttgg atcggttgat ttcttctttt
     5161 tctttatcca gtttgctagt ccccagccga tcttttttac catgtctgtc gtcaagttta
     5221 cttcatatcc ttcaactggt aatgcgattc ctcgaatatc cgatccattt tgcagcgctt
     5281 tcatatcttt catataaaag tcctcctcat ttattccctc attttttcca gcttttctgc
     5341 aaatcgctgt gcttcttctt ctgagcggca aataataatg aatgtatcta caccggccat
     5401 cgttccgaca acttcagggt aagccacttc atctagccaa ttcgttacca catcggcacc
     5461 atttcgttca gtcaatacga taaccatgaa ctgaacagac tggatacgca aaacctcgcg
     5521 tctaattgct gtacgcaaac gttcttcatt taaagaaaca gatggctggt taaataatgt
     5581 atagcgtact attttgtttt cattttgact ttttactaac ttcatttctc gaatgtctcg
     5641 agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa gcagctcttc
     5701 ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc gctgtttctt
     5761 tttcatatta gacacctctc aatgttgatt atagcaaaag ataagtaata tttcttcata
     5821 ttttggtatt ttttactttt attttttgtt ttcattcagg ttgctaacct ttttctattt
     5881 taacacgcaa aatcgtcttc attttttcag gtgctgattt tcttggatcg ctgatgtaaa
     5941 tctctttgtg ttctttagaa gctctttcat agccttcttt tgcgatgaat gcttctaatt
     6001 tctcaaacgt tgctggttct tcggcatagg gcccgacgtg taagatttgg gcacaaagac
     6061 cttcttccat ctttttgaat actacttgtt ctttcaagtc ttccggaagt ttgctagaac
     6121 gctgaatggc ttcttgtgct atcgcttctg taacaaagtc aggctgtcgg atcatcaatt
     6181 gataagaaaa atgttctttt ttcatcactg gttcatgcaa atacttttct tgcagtcccc
     6241 attgtccttc cagtggatag acagtataca tctgataatc aggaatcagc caattttttt
     6301 taggcgacat gcgtattgca tagctgactg catatagaca ggctactctt cgtttaaagt
     6361 cttcctgatt cgggtcgccg atccctctga tgctaaagaa tgatttttta ggaacctcta
     6421 gtatttgcgg gctttttttc atactatagc tcttgtcttt acgccattca attttttcca
     6481 tttttcttcc tccgcgaaat tccatttagt tatttattat catacctctt tttgagtgac
     6541 ttggctaatc gtgttgacac tatttttcag tttgtgctag aataagtgaa gtaaataaat
     6601 cgtacagata acacgtttcg gcgtgtcgag agagaggcgg tttctatttt tataatagaa
     6661 atcactctct ttttttacga aattttttag aggtgatgta aatgacaaaa caaaaacagc
     6721 tgcttttagt acctgcagta attgcaacag ggatgatgtc ttttgcgggc gtattgatcg
     6781 agacggccat gaatgtaact ttcccaactc ttatcagaca gtttgattta acgactgcac
     6841 aagtccaatg ggtaacaacg atttatcttt tgatgatttc gatcattgtt ccgctgtcta
     6901 cttatttgaa tcgcaatttt agtctcaaaa aactcttttt agcttcaaat ctgcttttct
     6961 tggcaggtat aatgatcgat ttcttctctc ctaccttcat ggtcctattg tttggtcgat
     7021 tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat gttccatatt atcttgacat
     7081 ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg tactttgaca acggccattg
     7141 ctccagcaat tggccccact tatggaggca ttatgacttc acattttaca tggaatcata
     7201 tcttcctgtt tcttgtgccc gttttgattc tttctttgat tatcggctgg tttgctattc
     7261 ccgagatggc agtacaaaaa agtggttatt tggattggat aagcgtttta ggaatttgtc
     7321 ttttgtttag tggaagtcta acatttttga gtacgttggg aacagtttcc agctggattg
     7381 ctttaatcat cggcggtatt ggctggttcc ttttttacta tcgaagtaaa aaagtggagc
     7441 atccactgat ccgactgtct attctttcaa ataaaacatt ccgcttgttt ttgattggct
     7501 tcttagttta tcagttttta ttacttggtg tatcttttgt cttgccgaat ttccttcaga
     7561 tcgttcaagg aagtagtcct tttgtcgctg gattagcgat gcttcccggc gcagcggttg
     7621 gtgctttgct ttctcctttt tccgggaaaa tgctggatca atatggtccg aagaagccaa
     7681 tttttgctgg gctgattctt tctttgattg gttggctagc attattccta ctcataggac
     7741 acgcttcagt gggtgtattg attgcttgtc atgtgtttta tatgatcggt atcggattat
     7801 cttacagcaa tatgatgacc accggaatga atgcactgaa tgaagaacta caaggagacg
     7861 ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc tgtggctact tcattagtag
     7921 cggtcattat caattacgtc cagcatcata cttcccataa ctatgaagtg tctactacat
     7981 taggatcaaa agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc tttgctcgat
     8041 tcgcctatta tctgttcttt gctaaaaaag cgtagattcc atatacggca ggtgttgcat
     8101 ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta tggaaaaaat
     8161 tgccattatc ggtggcggga tcattggtat gaccctagcc agttacttag atacatcaaa
     8221 attcaatgtt gctttatttg atgaagggac aggacaggca actaaagcta gcgctgggat
     8281 tatctcccct tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg ctaaagacgg
     8341 cgctgctttt ttccctaaac ttataaaaga tttccaactt gatcaaacga tttacgcaca
     8401 atcagggaca cttttgcttc gacctgaaaa agatttagct gaattggctg ttcttgccga
     8461 gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa tggctttcac ctgaacagac
     8521 atctcaagca cttccgttat tgaaaccagc agtttcactg cgtatcagtg gtggtggtcg
     8581 tctggatgga aaagcatatt tagaacatct tggtcagcgt ttagcacaaa aacaaatgga
     8641 gcagatccag gcaaaagtca ctttgcataa gaagagcgat tcttggctca tcaagggacc
     8701 gtttggagaa tacacagctg atcgtgtaat tttgacacct ggcccggcac ttaagccttt
     8761 attacaagaa ttaggctatg ctgcagatat acgtccgcaa aaagggcagc tcttagcatt
     8821 tcagacatct tataagatga gtcaagactg gcctgttgct atgcttgacg gagaagctga
     8881 tttcattcct ttcatggatg gaaagatcct tttaggtgct acccatgaaa acgatcaagg
     8941 ctgggatttg gctcctacta gcgaagctta taagggattg actaagagta cagaacagtt
     9001 cttgactgat ccagatcaga taaaaaactg gccatacgag ttccgtgtag gtacccgtgc
     9061 ttatacgtca gatttttccc catttttcgg cgtactgcca gatgatccaa gcttactggc
     9121 tgctagcggt ctaggctctt ctggcttgac cacaggtcct tatatcggct atctattagc
     9181 caacttctta aatactggtc attggcctac ggaagattat caaaaggaca tctccaccta
     9241 tattacgaaa aatccatcat agaaaaacag ctttttcaag taaggatccc gagcgatatt
     9301 gcgcatggat tacttcttga aaaagctgtt attttagttc aatttctgaa tcaaattctc
     9361 caaactttct tttgcttcat ctggcaagag atcttttcct atctgagctt caatgaatcg
     9421 taaacgaata tcaagtcgtt gtttcaccag atttttatag gacgtatcct caaaagaaac
     9481 aggaaaatct ggtataggta aatagtggat gtcttcaaat ggaccaaacg acttgaattc
     9541 tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt tgattttttt
     9601 atcatcgaaa tatccagtat tcaggacata gcaggctgtt tgctgtttca aaaacaactc
     9661 tcgaaaagct tgatagtctt ccctaagagg atagacacgg aaaggattag caaatggttc
     9721 aatgactagc tcatctagct gtctttttgt agtgatattt tccgcgtctg atcgttttgt
     9781 tgctaatgtt gcaccaaaag acgctgctaa aactgggtca ttgatcttga taattggcgg
     9841 aagcgaatca tctttcatga tccaaaaaac agcatcgatc ttttcgctaa gatgatcgac
     9901 tcggtcgggt gtcgcataac gtgatttgac tgtccgccca tttccgtttc gtatatctcc
     9961 tgttactagg actttttctc ctttacttgt acatgtcacg cccacattct gacaagtaag
    10021 gaaagaagaa atggccggat catcagacgg atagtcttgt gttttatcaa agtaagaagg
    10081 ttccaaggca gtagcaaaac cattttttcg agaaataacg aaagcatcgt catgcaagat
    10141 cgaaacagag tgaccttccg tatgttttgc taaggtaatc gttgattttc cagaaccgga
    10201 caacccaaat acagccatcg tataggtctg gtcattacta gcatattgtt tcatcccacc
    10261 gtggcaggca ataaagccat tgcgatttcc caatgcccaa gcaagcgtca aagtcgcttt
    10321 ttttaattct ccaaagtaac gtaatcctag cacaaccgct gtattatgga gagcatcaaa
    10381 caaaatgagt ccatttggaa atgtttcatg tgtccattcg ggatcacaaa agatataaag
    10441 atctccctca tcaaaggaaa cagacttttg ataagtctct aagatcttcg aattcgctgg
    10501 ctgaaaattc agcaaataag aatacaaatt gttctcaaat ccttctggaa tcagcaaatg
    10561 actttgcaca gaaaattctt cagataggcc aacgattact tttgtatggt aaaactggcg
    10621 cttgcgactt tgatacacag cttctcttaa aattttctcg tatttgcttt gttccttgtc
    10681 taagtcctca aagatccgcc gagcacttgc tgttcttccc acaatgcttc catcattgaa
    10741 tactagtgtc ctaacatcag ctggtaaatc tagttcttca gcatgtgaga ttgttaaatc
    10801 tgtgatgatc gttcctggac tcccttttgc tagttgataa gctgtcttcc tatcaaaaac
    10861 ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac tggataacag
    10921 tggatttcca cttgtgattt cgtttaattc gagactctct tttgtactca tggctcctcc
    10981 tcattcccta aacaaaaaaa cgttttcttt attatacatt attcgagttt ttatttgtac
    11041 tgctgcgctg tgggaagcaa acgagttatt tttcacacaa aaaaaccgag aacttgaata
    11101 aacaagtttc tcggtttcat cattttcctt ttatcggctt agtaccagcc gtttgctaac
    11161 cagaatgttt tagcagcatc ccatgaacca taacgagatg ttacatattg ttctgctaca
    11221 cgttcttgat tggcagctga atagtcgccg tttaagtatg aagcatctaa ttgataacgg
    11281 ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg attctttttg tgcgatccat
    11341 tcttttgcag agcttgaagt tggcgctgtt gcagcagctg gggcagcagg agctacttca
    11401 actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt tgtagatgct
    11461 gtattttctg ttgttttgtt ttctgttgtt gctgaagaat catttgaagt tgggattgtt
    11521 aattgttcac caacataaat gcggttgatg tttgcgatcg aatttttttc agcaattgca
    11581 tcgatcattg agttgtctcc tgcaaatttt tgtgagatct tagataatga atcacctgat
    11641 tttactgtat aaacttcatc agcgtgagca tttgtagttc ctacaaacat tgcagtagca
    11701 ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg aattcatatt aaactccttt
    11761 ttttctccgt tttttattct ttcgacgcta ttaactttat catttagaaa tatttcatag
    11821 gtaaaagaaa catgacattt aatgacaaaa taacgaatta tttaatattt gtaacattta
    11881 aacaaatatt tttttgatac aaaaccataa aagccttgtt gtataagcaa agtaatcgtt
    11941 tttctatgtt tttttatttt tgaatgaaaa acgcagattc tgtaacattc actgcaataa
    12001 tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa ttctcttaaa aaaatgtaaa
    12061 aaatacaaaa aaaccagtaa ttgttacaac aattactgga caaaaacttt gaataatgtt
    12121 aactattttt ttattttaac tttctccgca ataatttctg ggcatgttcc aattcgttta
    12181 taacgacttc ttctgtttct ttagctaata tttcatctaa tacatcaagc catttttccg
    12241 gttcttttgt tcctagacgt ccaatagccc aagctgctgt tcctctgatg acagggcgga
    12301 catctgttaa acattctgaa atctcaggca atgccgttcg atcacctaga ttagctaaag
    12361 caatcaatgc gtttctctgc aaaggtttct tcccacgcca tgaaccagct aaatgcccaa
    12421 attgctgttt gaagtcttta ttagacatcg aaagcattgg ctttaatttt ggatatattt
    12481 cgtctacttt tggttccatc tcttcatgaa aatgaaaatc ttttccttga ttgtaagggc
    12541 agactaactg gcagatatca catccataaa tgacatttcg cattttttta cgatattctt
    12601 ccggcatcat cccttttgtc tgagtttgat aagacaaaca tcgctgggca ttcattcttc
    12661 cgtcaccaag caatgctttt gtcggacaag cagtcacgca gcgcatacag tcaccgcaac
    12721 caaattctcc tggtttatca ggctcaaata caatattagt aacgatctca ccaagataga
    12781 caaatgaacc gaactcttca gtgatcaaca atccgtttcg tccaataaat cctaaaccag
    12841 ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc tacttgcgga gctagacgcc
    12901 attcctcttc tttttgccat ttttctgctc ttgtttggat gaattcgatc aatcggttca
    12961 aacggtctct gagtatatca tggtaatcga tcccccaaga agcacgagca aactgcccgc
    13021 gtttttcatc cctaggcact tcttcatgga tcctagtagg atacgcaagt gcaatggaaa
    13081 tgatcgattt cggctgatca aatgtcattt ctggataaag acgctcatcg atatttggat
    13141 gttcaaagcc agaagtatgg ccagctgctt tttgttcttc caacgattct tttaatgaat
    13201 caaaaggttc cgccgtggtg aacccgattt tgtcaattcc taatcgtttg ctttcttgta
    13261 tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg ttaacggctg tataacccat
    13321 aatatagttt ttcttttttc agctggacaa actggatctg ctgatcggca atcttctttt
    13381 cttccatcca tttttttctt gcagcaaata cttcccaata atttgttgaa attaattttc
    13441 ttttgttatc taatgcatta aaaaaattca atgaaactgg ttttgtactt aacagttcat
    13501 ggagccttac ctcttcccat ggcgaaaact cagacagatc gcgggaatac aatacttctt
    13561 catagagatt tttttgctgt gtgtacgtga atgtttgcgg gattttccca ggcctagcaa
    13621 tttctactag acgattataa aacatattca ttaatttgct gttggcaata aattgacttt
    13681 tataataggc cgactggatc tttttgatta ctccttctgc tttgatgact gaaaataatg
    13741 caggccatgc tttgacggtt atttcgtcgt tttgtttaac taatacgcca ggtgtgaagc
    13801 cgactggaga aaggaacaca aaataaccga taccggtatt ttcatctaaa ggctgaaatt
    13861 catcaaacaa ccatttgcct atgtacagct cttcatctgg ttctgttgtc tcattttgat
    13921 ttgctatgaa taatttagct aattctgctt caagaaattg aaggtcttga tccttgtcgg
    13981 tattttcctg ctggctgatc aatcgaaaga cttcttcttt aatcgtatat ttatctttac
    14041 gtgtaaaatt catagccaca ccttccttcc ttactctaac tataacggaa tttcttcaaa
    14101 aaaggtatct gtacgacgaa aaacagcata attttttttc atatctctta taaaaaatgc
    14161 aaggtctgct cctcaagcta gaccttgcac ttttcagctt tcttttgttt gtgacaaacc
    14221 ttcatggatc gtttcaatat tccatttcat catctgatag taagaatctc cttgttctcc
    14281 ttcttttgct atggaatcag tgaacagttt atcatagatt ttcaaacccg tttcttttgc
    14341 tacccgttcc atgcttcttg agtctacact ggtttccacg aataaaaccg gtacttcttt
    14401 tgctcttatc tgatcaatga tcgctttcat ttgatcaggc gtaccttgac tttctgtatt
    14461 gatctcccat atataagctg ctggcaaatc atatgctcgt gaaaaatact tgaatgcccc
    14521 ttcacttgtt actaataatt ttttgttctc ttcgatagaa gcaaaacttt ccttcgcttg
    14581 tgtatctaat tcttttagtt tttccacata tgcttctgca ttttttttat agagtgtcgc
    14641 attttctgca tctttttcag agaatatacg tgcgatttcc tccacatatt ttattccgtt
    14701 agataggtct agccaagcat ggggatctgc ttttgtatgt tcttcaccgc tagttaaata
    14761 aagaggttct acatttttgc taactgcaaa ataatctttt ccttcttctt ttttagccgt
    14821 ttccatcaag ttatcgaacc agctgttacc tgtttcaaga ttcaaaccgt tgtataaaat
    14881 aacatctgca tcgcttgcct ttttgatgtc ttctggtagt acttcatatt catgcggatc
    14941 tgttccgact ggtacgatac tgtggatatc tatttgatct gttccgacct cttttgccat
    15001 gtccgccaag atcgaattgg tagccacgac tgacaacttt tctttacttc cttcatccgc
    15061 ttggtcagaa gcctgctttc cgcaaccggc taataccaag aaactaactg ccaagattaa
    15121 aaagagtgat ttttttgttt tcatctatct gcttcctttc tgaaataaaa ttccttttgt
    15181 tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat gctaacacga tcgtagctcc
    15241 tgatggccaa ttgtaactat aactgaaaaa cacaccgaca actgcactta gcataccgat
    15301 tcctgcagct gtaatgatca ttttcagtaa atggttcgtt agcaagtaag ccgtggcggc
    15361 tggtgtgatc aacatggcaa tcaccaagat tgttcctact gtctgtaaac tgactacagc
    15421 cactaatgtc aaaaagaaca tcaaaagata atggatcagc gatgtgttca gcccataagc
    15481 ttttgccatt gtcggatcga atgatgtgat cttcaattct ttataaaaga aaaagacaaa
    15541 tatcaagacg attccactaa cgagtgctgt cagcaacaaa tcgctttccc taacagctaa
    15601 gacattccca aataaaatat gatacaaatc tgtcgcactc tgggcatatg aaatcaagat
    15661 aatccctaat gcaaaaaagg agctaaagac aattccaatc gctgtatcat ttttcaatcg
    15721 gctccgctga gtcacgaaac caatcactgc tgctgttagc atcccaaaag cagtggctcc
    15781 aaagatgtaa ctaaaaccaa acatataaga aatggctaca cctggaagaa ctgcgtgaga
    15841 gatagcgtct cccatcaaag acatccctcg taaaataata aaagaaccga tgactccaga
    15901 agcaagtcct acaagcatgg aagtgaccaa agcgtattgc aggaattgat aattcattaa
    15961 tccatcaata aaggattgga tcattttttc tctcctcctt ttactaaatg tctgatgatc
    16021 tcaccatagg ccaattgaag gttcttcgat gtaaagactt cttgtactgg accggaagcg
    16081 atcagctgtt tatttaaaag aataacctca tcgaaatatt cttctacttt atgaagatca
    16141 tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt cctgcaagat gtcaaagatc
    16201 tttcgttcac ttaacgcatc aatccctacg aatggttcat ctaaaaagat ccattctgct
    16261 ccttgggcta gagctcttgc aataaaaact ctctgaagct gtccacccga tagttcgctg
    16321 atctgtcttt tggcatattc ttccatccct acttttttca aagcttgtct tgcacgttct
    16381 ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg gatatgttcc taaaagtaca
    16441 acgcctaata catcaattgg aaaggaaaga tccaattcac ttcgctgttc cacgtaggct
    16501 atttttttac ggactgagtt taccgactgt ccagaaaagg tgactttacc agtagtagct
    16561 ggtatcaact ctagcaaaga tttcataaat gttgattttc ctgcaccgtt tggtccgata
    16621 ataccggtaa ttttttgtgg atttatcgtc aagttgatag ttttcaaaac agttttccct
    16681 tgataagcgg ctgaaaggtc ttgaattaca atggccgtac gttccaaatc ttacactcct
    16741 ttttaggttt acctaacttt ttatttaaat atagtttaac taaaatggaa agcattttca
    16801 agtagaatga tgaaaaagtt tttttcgaat tttcctcgca ttttatgaaa attttctgta
    16861 gaatcacatc aagagttcaa taaggctaac ctaataatga aaaacatgtg ttgaaacgtt
    16921 tgattatttt ttcttgttga ctcaacaacg aaaggagttt ttgtatggaa gaagaaaagt
    16981 taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc actaggtgga acgatcggtg
    17041 tcggtctgtt tatgggtgcc tcctcaacca tacgttggac agggccctca gtgatgggag
    17101 cctatgcgat tgcaggattc tttttgtatt tgattatgcg tgctttaggc gaaatgcttt
    17161 acgttgatcc ttcgactggt tcttttgcaa attacgcgag cgagtacatc catcctgtgg
    17221 ctggctactt gaccgcttgg agtaatattt ttcaatatat tgtcgtagga ataagtgaag
    17281 tgattgctgt aggatcttat atgaattatt ggtggcctga tttgccggca attattcccg
    17341 gaatcattgt cgtcttgttt cttatgctgg caaatcttat ttctgtcaag gcgtttggcg
    17401 aattagaatt ttggttttcg atcatcaaag tcataacgat cattttgatg atcattgcag
    17461 gtcttggggt aatcttattt ggatttggta atcatggtca ggcagtaggg atttcaaatc
    17521 tttggaaaaa tggtggattc ttcactggag gcgtcaaagg atttttcttt gcattatcga
    17581 tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat gactgctggt gaagcagaga
    17641 atcctaaaaa gacgatcatt gaagctgtcc agtccactat cggtcgaatc ttgatttttt
    17701 atattggtgc tatttttatc attgtgagta tctatccgtg gaatcaattg agtgagattg
    17761 gttcaccttt tgtacaaact ttttctaaag tcggcattac atttgctgca ggactgatta
    17821 attttgtcgt gattacagcg gcattatcag gttgcaattc agggattttc agcgcaagtc
    17881 gtatggtcta tacacttgcc ataaatggaa agatgtctaa aaaatttctg aaactatctc
    17941 gtcacggtgc gcctttttat cctgtcgtag caatctcagg tggaatctta attggtctga
    18001 tcttaaatta tcttctacca atgttcatca accagtcaga aaatattttc gttttcgtat
    18061 acagctcaag tattttgcct ggtatggttc cttgggtagt gattttgatc agcgagattc
    18121 gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc ttttaaaatg ccactatctc
    18181 cttatacgaa ttatgtgaca cttgcttttt tagctatcgt attgattttc atgtttatca
    18241 atcctgaaac gagaatctca ttgattattg gactcgtttt cttgctttac atgacgatcc
    18301 attactttgt acgcgaaaaa agaaccggaa aataaaaaaa gaacgaagct tcaccttagt
    18361 ggaagcttcg ttctttttta ttgcttttat caattcaatt ttagcgtctc accgggagtc
    18421 aggaaataat tctccatgct catcccattc agcgacaaga tcgtttctac tggtacgcca
    18481 gttcgttcgg ctatttgatt tggaccttct ccttccaaaa ctacggttgt cgcagcctgt
    18541 gaagtcgcgc tgttggtatt agtttcagag gaatctgttg tgtcttgtgt tgttcgagct
    18601 tgcatttcag aagcttctac cgactctgct gtctcagaat cagaagatgt tgtgtcttgt
    18661 gtgcttgttg actctgcttg aagagaggcg ctagtctgca aataaatatc ggaaatctta
    18721 gcgacatgat tcgtataatt agccgataat tgattgatca aagtatctaa gtctacaccg
    18781 tctttatttt ctgctgcctg atacgctgca cgtaattttc tgatcccttc gtcggagatt
    18841 gcttgaagct cacgtgcttt ttggttagca actgctgata aaccagctat tccctcttga
    18901 tttccctgaa tttctgtctg gtattcttca atcaatgcag gcgtcttttc ttcgatcttt
    18961 ttcgaataat catttacgat tgcttgaagt tgctctggca aaccatttgc tgttttttct
    19021 tcagaagttt gtgtctcttt tgttttagtt gatgactcac ttgtttgttt cgtctctgtt
    19081 ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt ctgacgaatt ttttccatga
    19141 aaatatagac ccgcaactcc tcctgctatg atcaaaaaca cggcaactgc tgccccaatc
    19201 atgatcatcc gcttacgagc tgcttgtttt tgctgtcttc ttcgggtcat ccgaacattt
    19261 tctccccact cattttcatc atacattttt ttcaccacca atttttatgt ataatagcgt
    19321 tatcaaacgc ttcatattct tattgtagca aataattgtc gctgtgcaca tttttatctt
    19381 tgttatttat aagaaaattt caccagttga gatgattcca agattgtccg tcgatttctc
    19441 cttgcagctg cattgttcgt tcgtctatct cagccatcag tttctttatt tcatataaga
    19501 gcgcacggtc ttgatattct gatttttcgc ttaattcatc taattgttgg atcagccaat
    19561 catttgtcat aatgaagttc ctttcatttc ttgcagtgta aaggtcaagt cttcgatctg
    19621 ctcttttgcc tcagtcagtg cttctacgat atatgcttgt ttttcagatt cttcttcctg
    19681 cattttctga ttttcctgta ctctttgaat aaaccagtca atccgctgag tcagcgatgg
    19741 tttgccttcc aatgagagat actgctgata agcaattagc ttctcccaac gtttttcctg
    19801 ggataaatac ataaattgtg ctaaaacgaa tggcggaaga taggtcatat ggaatttatt
    19861 tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta tattcttctt ttcctccagc
    19921 ttggtattct ttttccgcca cacctgttgt caatacgatt cctagttctt tcccttttaa
    19981 tacatgtgct gctcgttcca agacattatc ttgccaaatt tttaaatgag caggagaact
    20041 ataccagtaa aacggaaact ggaaaatgat ccgatcatgc tgggccaaga gctgtagttc
    20101 agtctctata tctatttttc cgtctggata acttgattcc aaatgatgcc aagtagctgg
    20161 taaaccagca gaagcttctt tgaaaaattg ctgatttaaa gattcttcga tcgttggatg
    20221 gctaatgatg ataagtgtac gcatcccttt tcctcctttt tagtcttcgc tttacagtgt
    20281 agcataaccg ccacaagttt cttttgccga tgcttgtcat tttaaccaga ctaatcaggt
    20341 tgtccaagaa actgcggttt gtttttccaa ctgttttttc aaggtatcca aagcaatatt
    20401 cttaaaattt tcgtttttca tacgtttacg atagttcgta gccgtttctc cggtactctt
    20461 tttgaacatt cggctaaata cgactagact attgaaccct acttgatagg caatactttc
    20521 cacggattca tccgtcagag aaagaagaaa cgcccctcga ttgatccgta ccccgatcaa
    20581 atattccttg atactggtac ccatatgatt tttgaatact cgcgacaaat ggctccgatt
    20641 gaccgctaaa tgctgggcga tttcctcaac agacaatttt tcagcataat gtgcctcgat
    20701 gtatttgact gtttcaacag ctaaagagga atgaaccttt tgtacaccac cgctgacctt
    20761 tccgccatct tttaaaagga ctgataaaaa acggtaggtt aatgctgtta actgtaattc
    20821 attttccaag gtttcttccg tatctttcat gcattctaaa ataatgtcga tatatttttg
    20881 tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa agcgactgcc gaatgatttc
    20941 ttctgcagca ctcccaccaa atgaaagcca gcaatacatc cacggatctt ctccatctga
    21001 taagtaaaac gtcgagtctc ctggacgaat caagaacaga tcaccttttt tcaattggaa
    21061 ttgttgattt ttcacatgat aagatccttt gccttccatc acgacatgca ggatgtaatc
    21121 attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt ttagagaaac cacaaaaatc
    21181 aaaatacaat tctttttcta cttgttgatg ttcctttttt aaaaaaatcg attgctccac
    21241 gtctatccct ccttactcct ttagtataat gcaacaaaaa gttaaataaa agtcacagaa
    21301 atatattaaa aaggaagcga tttcttgtta agtttaagtc attaaaagat ctttgttatt
    21361 cgttgtaacc ggtttactta gtattatata ttgaacagga gaggaataca gattatgaaa
    21421 agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt ttggacacga cacattctat
    21481 gttacattaa gcacttattt tatggtattc gtgacaagcc agatgttcgc aggaacagat
    21541 aaggcaacga atgcaaaaat gattgctaca gttacaagct tagttgtagg tatccgctta
    21601 gttgaaatta tttttgatcc tttgattggt ggcatcatcg ataatactcg tacaaaatac
    21661 ggtaaattca aaccatggtt agttatcggc ggcgttgtta gttccgtact cttggtagca
    21721 atctttacca atttctttgg cttagctact cataatacga ctttatttat catattattt
    21781 gcaatcgttt tcatcttatt ggatagtttt tattcattta aggatatcgc tttttggtca
    21841 atgattccag ctttgagtac ggaaacaaag gaacgtgaaa aatttgctac gtttgctcgt
    21901 ttcggctctt ctctaggtgc aaatggtact actctagtcg ttgtccctat cgttactttc
    21961 tttacctatc ttgttactca taaacatatt gaaggcgctt caggctggtt ctggttcggt
    22021 gtgattgctg ctgttatttc aggcggtact gcgattatca ctgccttggg cacaaaagaa
    22081 gtcgatactg cgatccgtca gcaaaatgaa aaagctggga tcaaagatgt gttcaaagca
    22141 gttgctcaaa atgatcagtt gatgtggctt gcccttgctt atatcgctta tgcgattgcc
    22201 aatgtagcaa caactgccgt attattctac ttcttcaaat atgtacttgg ccaaccaact
    22261 gaattttgga tcgttggtct agttgctgcc ttacttggtt tagtagctgt tccactattc
    22321 ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc ttggagggat cgcaatgatg
    22381 atttcttctt actttttctt tacgatcgca ggttcaagtt tgccaatcgt tatcatcggg
    22441 ctagtattgt tctacttccc tcaacaattg attttcttat ccgctttgat gacgatcaca
    22501 gactctgttg aatatggtca atggaaaaat ggtatccgta acgaagctgt tactctatca
    22561 attcgccctt tactagataa aatcgctggt gctttgtcta acgggatcgt agggtttgtc
    22621 gctatcgctt ctgggatgac tggtaatgct actgctgctg atatcactgc tcatggcgta
    22681 acgactttca aagcttatgc attctatgct cctgctgctt tgatgatttt ctccgctatt
    22741 atttttgctt ggaagattac attgacagag aaaaaacatg cacaaatcgt tgagaaattg
    22801 gaaaacaaat tggctccagc tgatacaatg gaagatgaac ttcacgatgc agtagttcct
    22861 tcccattaag aaaggatctt gaaaatgaag attacagaaa aagattttgg ccaaggttat
    22921 cacttgataa ccttggagaa caaaaacaag ctagcacttt ctatctctga tttaggtgct
    22981 agaatcgtca gtttaaaatc aaatgatcga gaattagttt tgggatttga caccgctgaa
    23041 gaatatatcg aaaaagatcc gtatatcggt gcttctatcg gtcgcaccgc agggagaatc
    23101 gaaaatggcc acttttctct caatggaaaa acttaccaat tagccacaga tccaaaaact
    23161 ggacataatc tgcatggtgg tgctcctgga tttgaattaa aaaaatggtc ttatgtcatt
    23221 ttgaatggcg aaaatgaagc ttctgtcatt tttactacta caagtcccga cggagaacat
    23281 ggttttccag gtacaatgga tgtggaaatc cgctatactt tgacaaaaga taatatctgg
    23341 cgagtgacct ctcgcggcac cagtgaccaa gatactttat tcaatccgac aaatcatgtt
    23401 tacttcaact tgacgggaga tgcttctcag tcaatcgatc aacatgagct ttggctcaat
    23461 agcgaagctt atgctccttt gcgaacagat agtattccta taggggtaaa agaaaatgct
    23521 gcaggaagtg ctttcgattt tcaaatacct aaaaaattag cgtccgtttt tgctagtgat
    23581 ttggatcaaa aaaatcttgt tgacggaatc gatcatccat ttttcttaaa agaaaccggc
    23641 ttgggtaaag aagccgctcg cttgactagc cccgataaaa gaatccaagt agatattgct
    23701 acagatgctt ctagtgtggt tattttcact gcaaatttcg gcacagaaac accagaaatg
    23761 cgcaaccgaa aactggcaca tcacggtggc atcacatttg agactcaaac agctccaggt
    23821 gcagagcgct tctcagcatt tggttcgatt catttaaaag ccggatcagt atttgaaaca
    23881 gtgacagaat tcaaaatcaa aacaagaaag gaataactca tcatggaaat caaaagaagc
    23941 ttaaacacaa aatttgccga aacattcgga cctaaaaaaa ctacacaata cttctcacca
    24001 ggacgcatca atttgattgg tgaacacaca gattacaatg gaggccatgt cttccccgct
    24061 tccatcactt atggaacgca aggtgtagca gcacctcgag acgataataa aatattagtt
    24121 tattccacaa actttgacga tatcggtgtc attagtttta ctttagatga gctagaatat
    24181 gataaaaaag caaactgggc aaattacgta aaaggaatgg tccttaaatt aaaagaagct
    24241 ggacactcaa tcgatcatgg gtttgagtta ttagtggaag gaacaatccc taatggtgca
    24301 ggactttcca gtagcgcttc attagaatta ttagtaggtg ttgtattaga agatctgttt
    24361 gatttaaaaa cagatcgctt ggaactagtc caaacaggta aaaaagtaga aaatgaattc
    24421 atcggtgtca attcaggtat catggatcag tttgccatcg gcttcggtga gctagatcat
    24481 gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg ttccagtgaa actagatggt
    24541 tatgcaatcg tcatcatgaa tacgaataag cggcgggaat tggcagattc aaaatacaat
    24601 gaacgccgta gcgaatgcga agaagccttg gcacgtttgc aaaaaacttt gcctatctct
    24661 tctttaggag acttagatga agaagaattt gaatcaacta ttgaccaaat cggcgatgat
    24721 acattgatac gcagagctcg ccatgccgtt tctgaaaatc agcggacttt gaaggcaaaa
    24781 gctgaattag aagctgggaa cttagaagca tttggtcaac tgttaaatga ttctcaccac
    24841 tctcttcgct atgattatga agtaactggg atcgaattag atacactagt agatgctgct
    24901 caaaaacaag aaggtgtctt aggtgcacga atgacaggag ctggatttgg tggctgtgcg
    24961 attgctcttg tcaaagaaga aaatatacct gccttcaaaa acaatgtcta tgatgaatac
    25021 atgaaagttg tcggctatgc tccggaattt tatgttgcac acattggaaa tgggacaaca
    25081 aagatcgatc cagaagctgt tcatgtttga taaaaaaatc aacgatctca gctaaaaaca
    25141 aatagataaa aataacctct cagcaatttc caaatgtgag ccttttgctc gtatttgaaa
    25201 attgctgttt tttattgaca cggaaagaga acgtgtgttc taattgtgtt gaagaggtga
    25261 ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga aaaagaacct tcaagagata
    25321 ttttatgtat tgactgcaaa tctttttacg cctccgtaga gtgcgttgaa aaggatcttg
    25381 atccccttac aactaaatta gtagtcatgt cctatccgtc cgattcgaca gaaacacggg
    25441 gtagcggatt aatcttggct tccagtcctg cagcaaaaaa agcttacggc atcaccaata
    25501 tcagccgtgc cagagatctt cccttcccct atccaagtga tttatatatt gttgccccaa
    25561 ggatggccta ttatatggaa aagaacagag aaatcaattc tatctataaa aaatatgcag
    25621 acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt ccttgatgta acaaatagca
    25681 tggcactctt tggtaaaaca aatgcgtacg acatggctaa actgatccat aaagatgtgt
    25741 ttgagcagac cggaatctac acgactatag gaattggcga taacccgcta cttgctaaac
    25801 tagccttaga caatggctca aaagacggaa agcaaatgtt tgctgaatgg cgttatgaag
    25861 atgtaccaga aaagttatgg aaaatctcag atatcactga cttttgggga atcggcaaac
    25921 gaacagcaaa acgtctgaaa caaatgggta tacattctgt ttatgattta gctcacgcgg
    25981 attattatca cttgaaaagc aagctagggg tcatcggtac tcagctttat gcccatgctt
    26041 ggggcatcga tcgaagtttt ttgggacaag aatatacacc aaaatcaaaa agtatcggaa
    26101 atagtcaaat cctagataga gattatacaa gacgagaaga aatcgagatc gttatcaaag
    26161 aaatggccga ccaagtcggc actcgcttga gacgatctgc agcaaaaaca caagtagtca
    26221 gcttatgggt aggatactct ttaggctatc ttgatccaaa tggcaagacc gggtttcacc
    26281 agcaaatgaa aatcgaagcg acaaatgcta gcagagatct agcagatgct ttattgacca
    26341 ttttccacca gcattaccac taccaggata ttcggcgtgt aggcgtgaac tgttccgacc
    26401 tcgtatacgc gacaggttta cagcttaacc tttttgaaga tcctgaaaag cagataaatg
    26461 aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa atacggcttt caatcaatca
    26521 tccatgcgca tagtctcttg caaggaggac gcgccatcgc acgtagttct ctcgttggcg
    26581 ggcatgcagg tggtatgagt ggattagaag gtggaaaaga tgagacggac agcaaaaaaa
    26641 ttcattgact ataacgagta tcgagatcgg ccatttggat taaaatgggg gactgctttt
    26701 gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag aagaagcacg caaacagcct
    26761 cttcctaaac aacaaatgac attgcaagaa atcgatagta tactgacaga agccttttta
    26821 tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg aatttggaag actcttggat
    26881 gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt actttatgat tgaccatcag
    26941 ccgatattgt gggaagacgt acggtatatt gagttaaagg aaacaaaaaa atggtttgtg
    27001 acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat aagaaaggat ttttcgaaaa
    27061 ttgcttctta aatttttgtg gaattttggc ttatttttcg aagaagcaac ttctggaaca
    27121 acgtatatta gatatcagag gttgtgaaaa agtcgcttag ttttgagtaa caagccaagg
    27181 cgctgatttc acaacctctg tctatctatc catctattcg gtaagattca tgcatgacgt
    27241 cttctttatt tagtatgtag agcccttttt cttctgacca ttcaacataa atcagctgtt
    27301 ttacatctgc atagccttct ttcttcaagt tctccataag ccatgcttca gtcaattgat
    27361 tggactgaag cactttatgc tgaatttgcc cttcatctac aagcaggatc gataaagctt
    27421 tgtcttcatc tgattttttc aatacactca tttgaccggc attctctagg ataacatgtt
    27481 tggcattttc aagaaaaaag cattcttgct gtcttaagat tgctcgcaac tgctccatct
    27541 cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc gtctttgatt agaacagatg
    27601 gctcgccttt gacccaacga ttgacctttt catttttttg gacgatacgt tcgataccat
    27661 aaatcattac tgcccaaagc gcaatggcaa ataggacatg ccctacatgg acattgtcat
    27721 cataggttga ttcttctaag atcccaccta aaacaagcgt atagactaaa tcaaacggtg
    27781 tgatttctga catggatttc ttcccaagca aacgtaccac taataataaa ccgatcaacc
    27841 cgaacaccag tttcattgta attgaaaaaa tcattttttc tccccctaac tatctgttaa
    27901 ttaaattata tatgctttct cacgaacacg catggaatat acaaggaaga aatgtatcct
    27961 tttactaaaa aaaggactgt gacactgtag tgacccccaa aagttagact tttttggagt
    28021 ggagaaatcc actccttttt tttatgctct aaaatgatat ttttacacaa ccgttacggt
    28081 tgttttacga aattggacga gactttg
//
LOCUS       DACDYM010000032        27211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDYM010000032 DACDYM010000000
VERSION     DACDYM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.032"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(11..379)
                     /gene="rplL"
                     /locus_tag="IWM59_001387"
     CDS             complement(11..379)
                     /gene="rplL"
                     /locus_tag="IWM59_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9979428.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(432..935)
                     /locus_tag="IWM59_001388"
     CDS             complement(432..935)
                     /locus_tag="IWM59_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9979429.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(1345..2034)
                     /gene="rplA"
                     /locus_tag="IWM59_001389"
     CDS             complement(1345..2034)
                     /gene="rplA"
                     /locus_tag="IWM59_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9979430.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(2138..2560)
                     /gene="rplK"
                     /locus_tag="IWM59_001390"
     CDS             complement(2138..2560)
                     /gene="rplK"
                     /locus_tag="IWM59_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9979431.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            2903..3571
                     /gene="sdaAB"
                     /locus_tag="IWM59_001391"
     CDS             2903..3571
                     /gene="sdaAB"
                     /locus_tag="IWM59_001391"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9979432.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            3584..4456
                     /gene="sdaAA"
                     /locus_tag="IWM59_001392"
     CDS             3584..4456
                     /gene="sdaAA"
                     /locus_tag="IWM59_001392"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9979433.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(4547..5566)
                     /locus_tag="IWM59_001393"
     CDS             complement(4547..5566)
                     /locus_tag="IWM59_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9979434.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(5739..6626)
                     /locus_tag="IWM59_001394"
     CDS             complement(5739..6626)
                     /locus_tag="IWM59_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9979435.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(6703..7248)
                     /gene="nusG"
                     /locus_tag="IWM59_001395"
     CDS             complement(6703..7248)
                     /gene="nusG"
                     /locus_tag="IWM59_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9979436.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(7349..7519)
                     /gene="secE"
                     /locus_tag="IWM59_001396"
     CDS             complement(7349..7519)
                     /gene="secE"
                     /locus_tag="IWM59_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9979437.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(7543..7695)
                     /gene="rpmG"
                     /locus_tag="IWM59_001397"
     CDS             complement(7543..7695)
                     /gene="rpmG"
                     /locus_tag="IWM59_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9979438.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            7857..8501
                     /locus_tag="IWM59_001398"
     CDS             7857..8501
                     /locus_tag="IWM59_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9979439.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(8557..9060)
                     /locus_tag="IWM59_001399"
     CDS             complement(8557..9060)
                     /locus_tag="IWM59_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9979440.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(9073..9119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            9657..10622
                     /locus_tag="IWM59_001400"
     CDS             9657..10622
                     /locus_tag="IWM59_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9979441.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(10721..11893)
                     /locus_tag="IWM59_001401"
     CDS             complement(10721..11893)
                     /locus_tag="IWM59_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979442.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(11880..13025)
                     /locus_tag="IWM59_001402"
     CDS             complement(11880..13025)
                     /locus_tag="IWM59_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979443.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(13039..13971)
                     /locus_tag="IWM59_001403"
     CDS             complement(13039..13971)
                     /locus_tag="IWM59_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979444.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            14088..15167
                     /locus_tag="IWM59_001404"
     CDS             14088..15167
                     /locus_tag="IWM59_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9979445.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            15164..15793
                     /locus_tag="IWM59_001405"
     CDS             15164..15793
                     /locus_tag="IWM59_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9979446.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(15903..16130)
                     /locus_tag="IWM59_001406"
     CDS             complement(15903..16130)
                     /locus_tag="IWM59_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9979447.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(16233..18821)
                     /locus_tag="IWM59_001407"
     CDS             complement(16233..18821)
                     /locus_tag="IWM59_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9979448.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(18841..19500)
                     /locus_tag="IWM59_001408"
     CDS             complement(18841..19500)
                     /locus_tag="IWM59_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9979449.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(19549..20052)
                     /gene="trmL"
                     /locus_tag="IWM59_001409"
     CDS             complement(19549..20052)
                     /gene="trmL"
                     /locus_tag="IWM59_001409"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9979450.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(20273..21139)
                     /locus_tag="IWM59_001410"
     CDS             complement(20273..21139)
                     /locus_tag="IWM59_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9979451.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            21248..21889
                     /locus_tag="IWM59_001411"
     CDS             21248..21889
                     /locus_tag="IWM59_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9979452.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(21951..23312)
                     /gene="mgtE"
                     /locus_tag="IWM59_001412"
     CDS             complement(21951..23312)
                     /gene="mgtE"
                     /locus_tag="IWM59_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9979453.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(23330..24229)
                     /locus_tag="IWM59_001413"
     CDS             complement(23330..24229)
                     /locus_tag="IWM59_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9979454.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(24231..25028)
                     /locus_tag="IWM59_001414"
     CDS             complement(24231..25028)
                     /locus_tag="IWM59_001414"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9979455.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(25006..25710)
                     /locus_tag="IWM59_001415"
     CDS             complement(25006..25710)
                     /locus_tag="IWM59_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9979456.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            25841..26416
                     /locus_tag="IWM59_001416"
     CDS             25841..26416
                     /locus_tag="IWM59_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9979457.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            26534..27211
                     /locus_tag="IWM59_001417"
     CDS             26534..27211
                     /locus_tag="IWM59_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9979458.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         7922 a         5744 c         4904 g         8641 t
ORIGIN      
        1 atagtaaaaa ttatttaaca gttacagatg cgccaacttc ttcaagttta gctttcaatt
       61 cttcagcttc ttctttagat acgccttctt taactggtgc aggagcgcca tcaactacag
      121 ctttagcttc tttcaagcct aaaccagttg cttcacgaac tgctttgata actttaactt
      181 tttggtctcc agctgcagtt aattctacag tgaattctgt ttgttcttca gcagcagcag
      241 cgccaccagc agcagctaca gctacaggag cagcagcaga tacgccaaat tcttcttcaa
      301 tagctttaac taggtcgtta agttctagga tagttgcttc ttttaactca gcaacgatgt
      361 tttcaatgtt caatgccatt ataatttcct ccgtttaatt gttctttttt atttaatgct
      421 ttatgcggca attaagctgc atctccatct tctttgtctg ccactgcttt gacagcgtaa
      481 gccacgttgc ggactggcgc ttgtagtaca gatagcaaca tagatagaag tccttcgcga
      541 tttggcaatt ttgccaatgc tgtgatttct tccaatgaag agactttacc ttcgatgata
      601 ccgcctttga tttctaatgc ttttgcttct ttagcaaatt cgtccatgat tttcgctggt
      661 gcgactacat cttcgttact gaatgcaaca gctgtaggtc cagtgaatac ttcgtccatg
      721 ccttcaaggc cagctttttt agctgcacgt gaaagaatag aatttttgat aactttcatt
      781 tctacgttag catcacgaag ttgtttacgt aaacgtgtta cttcatcaac tgttaatccg
      841 cggtagtcaa cgactactac tgatgcagct gctgtaaatt tttcgcttac ttcatcaacg
      901 attgctgctt tttttgcgat aattgcttca ctcatttttg agtttcacct cctgaatttt
      961 gatggaagag ttttttgcac tgaaaaggca agtcactttt atagttactt tgctcaacca
     1021 gtattattca tcacctattc acttcgttca tagtgattct actcgctttg ctcgaaagga
     1081 aactcctttc gctttgctca accagtattg ttcatcatct cttcacttcg ttcatagtga
     1141 ttcacaataa aaaactctat gccaccgcag acatagaggg actattgaat atattcaata
     1201 aacgtcctcg gtaggaaatt aaggcttgat gccacctact gtcttcggta caagttaatt
     1261 attaacctca actagcttag catgctaaac tagttgagtc aagaattatt tttcagttgg
     1321 atcaatcaac attctaatat ttaattagaa agaagcttgg tcaacatgga taccaggtcc
     1381 aaatgttgtt gtgatcgtta catttttcat gtattgacct ttagctgctg atggtttagc
     1441 ttttagcaat acatcatgga ttgctgcaaa gttttcaact aatttttcat cgtcaaatga
     1501 aacttttccg attggtacat ggatatttcc agctttgtcc acacggtaag ttactttacc
     1561 agctttcact tcgtttactg cttttgttac gtccattgta actgtaccag ttttagggtt
     1621 tggcattaag ccttttggtc ctaatactcg tcctagacga cctacagtag ccatcatatc
     1681 aggtgttgca acaactacat caaagtcaaa ccatccgttt tggatttttt gaaccatgtc
     1741 gtcgtcacct acgaaatctg caccagctgc ttcagcttct tttgctttgt ctcctttagc
     1801 aaaaactaaa acagtttgtg ttttaccagt tccgtttggt aatacaacag caccacggat
     1861 ttgttggtct gcttttttag ggtctacgtt caaacggtat gcaacttcaa ctgttgcatc
     1921 aaattttgca atgtttgttt cttttgctaa agctactgct tcggcaacag gataaacttt
     1981 acttgaatca acttttttta atgcatcttg catttttttg ctctttttag ccattttggt
     2041 tcctccttga ttgtggttat aacggttgta cctcccacgt gttctatatc tttcaatata
     2101 gagccaactg agaagctgct gtcttagggt gtatagatta ttctacagtg atccccatgc
     2161 ttcgtgcagt tccttcaacc atgcgcattg ctgcttcaac gttagctgcg tttaggtctt
     2221 ccatttttaa ttcagcgatt tctcttactt gatcgctaga aactttcgca actttatttt
     2281 tgtttggttc gcctgaacct ttttcgattt tagctgcttt tttcaataga acagcagctg
     2341 gcggtgtttt tgtaacgaat gtgaatgaac ggtcttcgta tacagaaata acaaccggaa
     2401 tgatcaaacc tgcttgatca gctgtacgag cgttgaattc ttttgtgaat cccatgatat
     2461 tgatacccgc ttgacctagt gcaggaccta ctggtggagc tggagttgct ttacctgcag
     2521 gaatttgcaa tttaacgatt ttttctactt tctttgccac gagacatacc tccttaagtc
     2581 cgtgatgtgg ttaattggag atgcatattt ctcctcccac gttttatctg atctgaaaaa
     2641 atccagacaa ataatgtgca aaacaatgca cactggtaca gtatagcaaa tttatttgtt
     2701 ttttcaagat ttttttctgc tatttttaca ttttgcttga ataaacgcta aatttattct
     2761 aaaaaaacaa caatttttcg ttctcatttc ttcttttatg ttacacttgc gctagcatgg
     2821 ttcaacagat gaaagcaaac aataacacgg ttttttagac ccttgtacaa agattcctac
     2881 ataacaaaat gaggtggaga aaatgaaaga tttaaggtat agaagtgtct ttgatattat
     2941 tggacctgtg atgattggtc caagtagctc tcacactgcc ggagctgcta gaatcgggaa
     3001 aatcatacgt agtatatttg gcgaacagcc tgattctgta gatatttatc tatatgaatc
     3061 atttgcaaaa acctatcggg ggcacggaac agatattgca ttagttggcg gcctgttagg
     3121 gatggaaccc gatgatgaac gtttagctga ttctttgaaa ttggcatatg aagccggtat
     3181 ggaagtcttg tttgttccta agagtgaaaa agccgaacat ccaaactctg taaagatgct
     3241 tctaaaaaaa ggaacacgaa agctttcagt cactggcatt tcgatcggcg gtggcaatat
     3301 ccagatttct gaattgaatg gattcaaaat ttctttaagc atgggtactc ctaccttgat
     3361 cgttgtccac caagatgttc caggtatgat tgctaaagtg actagtatac tttctgagac
     3421 caatacgaat atcggcacta tgacagtgac acgggaatca aaaggtgaaa atgccattat
     3481 gataattgaa gtagatgatc ctcaagtaga agaaacggta aaaagactca aacagctgcc
     3541 aaatatcgac agcgtaaatt atttcgatta aggagctaaa aaaatgtttt tatcgattga
     3601 agaattagta gatcaagcaa aagcttatcc aagtgtagca gaattgatga ttgcagttga
     3661 aatggaaatg agtggccgtt cccgtagtca catcatcgag atcatggaaa aaaacctctc
     3721 agtaatggaa caatcaatca acgagggaaa agaaggtgtt acctccgtca caggaattac
     3781 cggtggtgat gcaaaaaaat tagatgatta tttaaatgag gcagactttt taagtggaga
     3841 aacaatctta aaagctgtac gcaatgctgt agctgttaat gaagtcaatg caaaaatggg
     3901 gttgatttgt gctacaccga ctgccggaag cgcgggtgtt gttcctggtg tgctgatggc
     3961 tgtccgagat cggcttgatt tgacacatga acagcaattg gaattcttat ttaccgctgg
     4021 cgctttcggg ttagtcatcg caaacaacgc ttctatcagt ggtgcagaag ggggatgtca
     4081 agcagaagtt ggttctgcaa gtgcaatggc agcagcagct ttggtttgtg caaaaaatgg
     4141 gactcctcat caagcagcac aagcagtagc cattacattg aaaaatatga tgggactgat
     4201 ttgcgatccc gttgcaggct tagttgaagt cccgtgtgtc aaacgtaatg ctttaggctc
     4261 ttctcaagct tttatctctg ctgatatggc attagcgggt atcgaaagtg tcattccgcc
     4321 ggatgaagtc gttgctgcaa tgtaccaagt cggacgtcaa atgccgtcga ttttcaaaga
     4381 aacagcagaa ggtggcttag ctgttacgcc aactgctaaa cgacttgcgg aagagatttt
     4441 aaataagagg gcgtgataaa gctccttaat ttcgagtatt aagagattag ttatcttcat
     4501 aacaaaaaac gaagagagga ctaattgccc ttctcttcgt tttttattaa tccatcaatt
     4561 tcgtattttt tacttcatcg atcgtttttg ttcctgcaag ttgcatcgtg atcgacaatt
     4621 ctttgttcag gtgttcaaaa acagatgtta ctccttctgc cccacctaga ttcaacccat
     4681 aaatcactgg tcggccaatc gcaactaagt cagcaccact tgccagagct ttgaagacat
     4741 gctctcctcg acggacacca gagtcgaaaa tcactggtac acgtttgttg actacttcgg
     4801 caatttttgg caatacttca aaggacgcag gacctccatc taattgacgt ccaccatgat
     4861 tggataccca gataccatct gcccctgcag agatagcgat tactgcatct tctggcgatt
     4921 gtattccttt tacgataact ggaagatggg tgatttcttt gattgttttg atgtcatctg
     4981 gtgtcaagcc ttgttttgca gctgcataaa tttctgcgat tccttttcct tcgccattac
     5041 cacttgcact ttgttcgcta tacgcagcta agttaggcat tggtaatgga aattggaatt
     5101 gattgatgac gtcttcttca cggtaaccac cgagcgttga atctgccgtt aaaatgatag
     5161 ctttagcccc tgcttctact gctttgttca aaatgaactc attgaatctg tcgtctttac
     5221 tcatatataa ttggaaaaac tgcggtgcgc ctggcgctgc atcggctgcg tctttgattg
     5281 tcgtatttgc ataagtgctg atggaaaaga ttgatccagc agcagctact cctttagctg
     5341 tatcagcttc ccctttttca tgtgccaatc cttgggccgc tgatggcgct tggataatcg
     5401 gtgtatctaa ttcaattccg aaaacagatg tatgcaaatc ggctgagtcg attccgcgta
     5461 atatccgtgg catgattttc tttgtattaa aagaagtcgt attttctttc atcgtccact
     5521 catcttcaga accaccgcga atatagccaa aagctcctgc ttccatgcgc tcttttacac
     5581 gaccttctag ttaggcaatg tttacaatat cgattggatg ttcttcatta cttgtatgat
     5641 aagtcatagt agatccttct ttcatcttct tgattacata taaatcatac taaacttaca
     5701 aaaagtaagc aaacatttac tctcagatac ttttatcgct attctttcca aataaatcga
     5761 cttacaagct gatctacttc ttggttttca tgaagcatac tatgagaggc attttcacct
     5821 gtcactactt tttcctctat ttcatttccg cgttgcttca acaacgcata gaccgctaat
     5881 gcactgttta acggtactgt tccatcactc aagtctgttt catctagttg accagcaatc
     5941 aagaaaaatc gagtggttat aggaatatta ccgatcattt gctggtaatc ttgatagcgg
     6001 ttagaaacga cagcaggccc ctttgctaac tcatctgtca gagactgcgc actatcatcg
     6061 acaaaatcat taaaaggcgc gccaattgct gttaatgtct gaacttgtgg caatgacgta
     6121 tcctgtccat acgttcctaa atagcgtagt gcactaactc ctcccatgga atgccccact
     6181 atgttgactt tatctatgcc gtagttttct ttcagataca aaagacaagt tttgatccat
     6241 tctgcttgat tccactcgtt atttttatta tcttcaaaca atacttggac catcggattg
     6301 tctgcttttc cagacaaatc tcctgttgct tgtatttgtc catctgttcc tactgttaaa
     6361 accaattctt ttactgcttg ctgatttttt tccaaacgcc gcaacatccc tttaaaggaa
     6421 ttttctgtcc cgctgtagcc gtggaaaaaa agtgttggaa tagtggtttt ttctatcact
     6481 ttactggatt ctgccgtttt ttctttatca gaaacttcag tagtatttat cttcgtcgtc
     6541 tggcaaccac ttaacgcaaa caaactgata aggactaaag ctgctgcagt tgcacgtttg
     6601 cataaatttt tgattggttc tgtcatacac ctcttccttt ccatatagaa aaaagaaaga
     6661 cttgaaaggc caatcatatg atcgacattt caagtctctt tattaatcga ttttatctac
     6721 ttgatcgaaa tcaagttctg tactggtttc acgtccaaac atatcgatgt tgactttcaa
     6781 tttttctttt tctccgtcaa cttctgttac ttgtccttct aatccagcaa atgcgccttc
     6841 gatgattttc acgacttcgc caacttctac ttctagatca ttttgtcgca ggctcatacc
     6901 gatagaacgc aagatatgat tgacttcttc tggaagtaat ggtgctggtt tgcttccggc
     6961 tccgtgtgaa ccaacgaatc ctgtcacacc aggtgtgttg cggacaacat accatgaagc
     7021 gtctgtcatg atcatttcaa caagtacgta tcctgggaat gttttatgaa cgatttcttt
     7081 ttctttccca tttttgactt cagtttcttt ttcttcagga acaacgacac ggaaaataaa
     7141 gtcggccatt ttcatacttt gcgcacgaga ttcgatattt gcttttactt tgttttcata
     7201 tcctgaatat gtatgcaaga cataccagtt tttttcaaaa gattccattg aaggctcctc
     7261 atttcattgt aaaataaaaa aacctcagta cacccgaagt ttttctttcg cttcattata
     7321 gcactaaaag tacgttgtgg ctagcgagtt attttaaaat ccagccaaat aagctttgga
     7381 tggctgtatc cattacgtaa aacatcaatc cgaaaataat agaagtttcg atgactacta
     7441 acgtatcttt acggagttgt tttctgcttg gccaagttac ttgtttgatt tcatctttta
     7501 cgctttttag aaatttcatg agacaacctc ccttgctact gtttatttcg tttctttatg
     7561 taatgtatat tgattacaat atttacaaaa cttattgatt tccaaacgtt cgccgctttt
     7621 tccttcactg actgattttg tgtagttgcg agacccacaa acggaacaag cgagtgctgc
     7681 ttttttcttt gccatttact tctgccccct tgctgagaat cagacttata cttcataaca
     7741 gtatcacgta actttttact tgtcaatttg tgaaataaac tatctattgt tttggataaa
     7801 attatgatat gattaatttg aaattaagct aaaatgagac aattggaggt atagcgatgc
     7861 tattaaaaac acttgtatat aagaaaaaag atctgacaac cgttacagag actagtacat
     7921 tagaagaagc attaaaaatt ttagaaactt ccggataccg ttgtgtgccg atcttagatt
     7981 cttcaggtaa tattttccgt ggaaacattt acaaaatgca tatctatcgt cataaagcaa
     8041 atggcggtga tatgacgctt cctgtcactc atcttttgaa aaacgctaca aagttcattt
     8101 acctggatac ttctttcttc aaagtattct ttacgatcaa agagcttcct tatattgctg
     8161 tccttgacag tgaaaatcat ttttatggta ttttgacaca tagtacgttg cttaatatgc
     8221 tctctcaatc ttggagcgta gaacaaggaa gctatgtact gacaattgct tccacaggaa
     8281 agcaaggaga tttggctgct attgcaaaaa tcatttcaaa acacagcagc attgccagct
     8341 gtattactct tgatatcgaa aaagacgaat acattcgacg tacattgatc acgctaccgg
     8401 ctaacactga agcgaaagta tgtgaaacga tcgtcaacca cttagagaaa aagaattata
     8461 aagtggtaga aattgaaaat ttgcaaaacc aagaagcata atcaaaaagc gaaaaaagaa
     8521 aacagctaat ctgctttctt ttttcgcttt ttataattag acacgctttt caaaatcaag
     8581 cgcgcgatct aaataataca tcaatggtgc tgaaacagcc atgacgataa actcacttaa
     8641 tgcaagcgtg ccataggttg tccaaaatgc ttggccacct gttggaacta acatataagc
     8701 aatcagtccc atgctgatgg taaagaagat cgtgttcaat actaaacgca tctttacatt
     8761 tttcactttg ttttgcaata aagctgtcag gcctaaagaa agcaatgttt ggccgccacc
     8821 aaaaaccaca tccatgatac caaaaccaaa gaaggcatta tagacgatga ccccgcctaa
     8881 aactccccaa agcaatttac ggttaaagac aactaaatga ttcaaacttt ctgaaatacg
     8941 aaactggatc ggccctgagg atacaggcgc aactaaaata gttaacgcta gataaagagc
     9001 catgatgatg ccatttacaa cgattacgga taaccgttgt ctgcttttcg ttttttgcat
     9061 tttctctcct cttttctcct agttttttta acgcgggatg gttgatgaac cgcggttatg
     9121 tgaaacaaac aataattata acagactttt catttatttc aatccgtctt atctgctttt
     9181 aaaaaagcaa aagcttaaac aatgtaaacg tttgctaaaa atcgtgggat atggtataaa
     9241 gtaagtgtaa ggggtcataa gaattcccag taaagcagac gaagtaggat ctgcaagaac
     9301 agaattgttg tgaaacttaa ataaataaaa aaggatgtgg gaatttgagc aacctcttac
     9361 tagagaacgg tctagcaagc agctgaagat ctaagtggac atgccgaacc ttggaacttc
     9421 tccttacgta agtaatggat catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg
     9481 acctttttca ttttcctaga cttctgctat aataaccaag aattaatttt gtaacagatt
     9541 tttttgaaaa aacaagtttc tttcattcaa aaaatagaaa aagcttgcta attattggtt
     9601 aaccagcaaa caggagtatt aaaatatctt gttttattga atagcaggag gaaatcatga
     9661 aattccatta tcatatattg atcaatccat cggcaggcag cggaaatggc tataaagtag
     9721 cagaaagaat tttaccggta ttaaaaaata agcatattga ctatacactt cattatagtg
     9781 aatacaaagg acacgatgct gagattgctg aaactttggc aaaagaaaca ttgattcctt
     9841 ggatagaaga agaacagaaa acaatcgata ctttccccct tttgatcatc gtaggaggtg
     9901 acggcacctt acatcaggta cttgatactt tctatcagat ggaagtagaa ttcccagttg
     9961 cttatattcc agcaggttct ggaaatgatt ttgctcgagg ggcaggcctt ccaaaaaatc
    10021 ccaaaaaagg acttcagctg attttagcag ctcaatcacc agaaaaagtc cacataatgg
    10081 cttatgaaga aaaaataagt gaaaaaaaag gaatagctgt taataatttc ggtataggat
    10141 tagatgcagc tatcgtccat gcgacaaatc attcttccac aaaaaagcga ttgaacaaat
    10201 acaacttagg atcattttcg tatttattct ctattctccg tgctttattc actcaaaaag
    10261 gatttccaat tctggtagat gcaggaggaa aacaattaag tttttcaaac gcatttttat
    10321 gtacagcaac gaagcatcct ttttttggcg gaggaatcgc gattgttcca actgctgatg
    10381 cgtctaaacc agtcattgat tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt
    10441 ggctcattct tttacttata cagaaaaaac aattgaaatc gaaatatttc catcactaca
    10501 caacaaacag attacgtatc gtttcaacaa caccgcaaca tggtcaagaa gatggagaag
    10561 aaatggaaaa acgacctttt gatatcacaa taaccacaac taaacagttg ttttggtgtt
    10621 aataaaagag gttttgtcag aagctacatc tggaacaccg tttattcgga aaaaaggact
    10681 gtgacacgac ttttgtcaca gtcctttttt tactatgcag ttaattcttt gcccaattgg
    10741 gttgccatcg atagtttctt tctttaccca aaataaattg gatcattgaa aacaccgcag
    10801 caaaagcaac taagacacaa ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac
    10861 tgagtgtttc actgatcaag ttattatttt gaacaaacca atatgttggt gtgaatgccg
    10921 ctattttgct aaccatttct ggcaagtatt ctgctggtac aaacacccca ccgataaagc
    10981 aactgcctgt gataaaaata tctttgatcc caccgctggt ttctgagttt tttaccaatg
    11041 aagtgataca agccgaaaag ctaatgatcg gcaataaaaa caagaaagta tttaaaatat
    11101 gataccatac cgttatatcc cacgaggtgt ggatcactaa cagaaccatt agaagtaaga
    11161 agccactgaa caacagcagc gagtaactaa tcaaactaaa ggttactttg cgattcaagt
    11221 ttcgtttggt aatgggcgca ctctggtttc gtttactgat ctctttgcga ttaaacgcta
    11281 gattgatgac gctgaaaccg ctaaaaatgg atagaaacaa cccataagac aagaaattaa
    11341 aggttgtgcc cgtcaacagc tttttcatcc gctgactata tcccgcactt agctcaactt
    11401 ctccagtaac cgagagtgcc atttgtgtct gttccatagc tgcatcgatt gttttatctt
    11461 cttcttgaaa cagaatcagt gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt
    11521 tagaaaaggc atctgggcga ctctgtatct tgatcgtcgc catctttcct acttcgactt
    11581 gttcggtaaa gtctgcaggc agatgtaaga taatgtttac acgattaaaa taaagagcat
    11641 catcgatttc tttttgtgag gttccatcca atgtaacgac tgtttccttg tcattcaaat
    11701 atttacgaag gccatcgact acaggcgact cttcttcact aatcaaggcg attttcgcat
    11761 cttgcagaga ggtattttca acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag
    11821 tgataattac acctatcagt aaattcgatt tatttgcttt aagcaatgtc caaatcgttt
    11881 tataaagcgt cataacgcac ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa
    11941 ataaatccga tcaaccatag aagattggta taaaaactag ccaaatcagt gtagaaataa
    12001 agtttataca aactttcgct gatcaagttg acgagattta attgaccaac aatcggaata
    12061 tgaagatcaa tccaatactt cacttgttcc gaccccatca taccagcaaa aaagctcatc
    12121 gcaatcgtca ctgaaatcaa gatgctatcc ttttgcacca gattcatttt tggtatcaaa
    12181 ttaccaacca atgtacctaa caaaatcgca cacaacgacc ccagagcggt aacaatcatc
    12241 aaccattgcc aacgttcacc aaagtctact tgatagataa aacgaaagac agctaacata
    12301 atataaatct ctgtcaaaac aatcgtaaag cttgctgcca aattagcgat tgaaactaac
    12361 gattgctttc taggaatcaa ggacaagcga ataccatttg acgattgatt tgcttgctga
    12421 tcctgtacat tgcggacccc ccacatcgtc ccgtagagaa tcgccattcc aatcagagtg
    12481 aagaaataaa aacttttgaa actcatatta cgacctgcat gaacttcttg aaaagatact
    12541 tcttgttcaa aaacagtcat gatgggcgcg agatcaacgt tctccacttc tgccacttta
    12601 ctcgaaatta taccaatacg ctgtaaaaat tgatccatga cgtttttcat caacgtctga
    12661 ctcattcctg cttgacccac atggagcgaa acagatgaac catcaatgag ataaaaacca
    12721 gccaaatcat ctgcatttag cttttcattc gcttctactt ccgatagttc ctgtacttca
    12781 aataatggtt tatcattgac ttcgacttgt tctaagactg tggtaaaatt ttccgaaagc
    12841 gctgtttgat ccgctttgat tacaattcca gtattaatcg tttcaagatt gccaccttga
    12901 tcaatctcgc caaaggccgc cataaacatc aatcctaaaa gaattggaaa ggccaatgtc
    12961 caaaacaaca tggatttatt tcgcagcagt atttttgtgc gatatttata aaaatgccaa
    13021 aacattattt cccctcctct agtctctcaa atccttgcca gtaatctcta aaaagacatc
    13081 attcaaggac gcttctttgg ttgctatttt tccgtaactg atcttttctt cttgtaagat
    13141 tgtaagaatc ggcaacaata ctgtatttag ttcagtagtt gttacttgga gctgaccttt
    13201 tttataagcg attccatcag aagctattgt tcgctgaatc gctgacaaca cattttctgg
    13261 gagtaatggg atgtcgatcg tcaaacgatc cgccgtccgt accatatctt ttagagattc
    13321 tttggttcct ttagcaatca cctgtccttg atccatgatc acgatctggt cgcaaagaat
    13381 ttctacttct tccatgtaat gtgttgtata gacaatggtt gctccttgtc gattcagctc
    13441 cttgatactt tctaaaattt tattccgact ttgggggtct acagctactg tcggctcatc
    13501 taaaaaaatt aaccgcggct tgtgggcaat cccacatgcg atgttcaacc gacgtttcaa
    13561 gccaccacta agttctcctg gacgaaacga aacatatttt tctaatccca ctaattgaat
    13621 cacctcttca atcaaggctt tccttttctc tttttcggaa acatataaac cgcaaaaata
    13681 atcgatattt tcttgcacac ttagttcgtc aaagaccgca acgtcttgag gaacgacccc
    13741 aatcattgcc ttgacagcat aattttcagg agtcattgtt gtcccgaaca atttaatggt
    13801 tcctttgtca taatttaaca aagaaagcat acagttgatg gcagtcgatt tcccactgcc
    13861 gtttggacct aacagaccta agacttctcc ttcttgtact tgcagatcaa aatgattcaa
    13921 tgctacaaaa tctccatagc gtttgactaa tttatcaatt tcgataatca tcatgaaaaa
    13981 ctccttccaa atttcttgtt gatctgacta taataaagtg caagaaaaga aactagtgac
    14041 gattggctaa accacacatg acatttgtca caatgaagga gtctgctatg cataaatctc
    14101 ggctactcaa cgaaactagc tttctactgg ttatctcatt gcttttgctg gtaagtcaag
    14161 gattcgttgc tatgcaagtt atctatttat tgatcgccgt tttattcact ggtgtcagtt
    14221 atttactgcc tacaaagcca aattggattt tccaagttat tctatttctt ttatgtgcct
    14281 ttttacctga tcttgtcctg tttctgccag cgacgatgcg agtgatcgcc cctgaagaaa
    14341 gccaccttga ttacttgttt ttgttaggtt ttttgttgat ttcgacggtt ggtaatcatc
    14401 cccccatcaa ggtcgccttt gtcgtaattt tgctgttttt ttccttttac ttacgaaaaa
    14461 aagatcaacg aacgaccgat cttgagaaaa agtacattgt ctccaaagat actcattggg
    14521 aaaaacaaca agagttatcg gaacaaaata aagcattggc cctgtcacaa gaaacacaat
    14581 tagccttgca aattgtggaa gaacggaacc gaattgcccg agacatccat gacaatgtcg
    14641 gtcatctgct gtcaagtgcg attttacaaa ttggtgcgct tgaaatgatc aatcaagaac
    14701 caaaactaaa agaaccatta gaaaatttaa gtgccaccgt tcacactggg atggatcgga
    14761 ttcgtcaaag tgtccatgac ctccatgaga catcgatctc ttttcaacac tccttagagt
    14821 ttctgatcgc tgattttcct tcttctgtta cgattgaagg aaacttgttt gaaggattga
    14881 atgaaacgca gcaaaactgc ttcttaatgg tcatcaaaga agccttaacc aacagtatca
    14941 aacatagtca tgccaaaacc gttgttttac attttcgaac gttgccggcc ttttatcgct
    15001 tgcaaatcac gaatgatggc accagcccac aaaaaaattc tgctcccggt attggtttga
    15061 aaacgatgcg tcaacgtgtt caagaacaaa agggacaagt ccatattttc caagaaaacc
    15121 agcaatttca gttaacgatc attttaccaa gggagggaca ccaatgataa cagtagtcat
    15181 tattgatgat gatccatttg tgacgaaatc gttacaaacc attttagaaa gcacgaaaga
    15241 gatccgtgtg ttaggcattg gtcattgtgc caaggatgcg ctagtcttat acgaaaccca
    15301 tcgtcccgat gttttgctga cggacatccg catgcctaaa caaacgggca tcgatgctgc
    15361 caaagaaatt ttggctaaat ttcccaaagc catccttttg cttttgacga cattcaaaga
    15421 tgaagaatac atccgcgaag cattttccat cggtgtcaaa gggtatttaa tcaaacagaa
    15481 cttgcaagcg attattcctt ctgtcaaatc cgcttataat ggacaggccg tatttggtaa
    15541 tgaaatcatc gagactttca ctcaaatgat gaagaataaa ccgacgattg atagtcatgc
    15601 ttatgcttct tttagtgaac gtgagcttgc aatcatcaaa gaagtagctg ccgggaaaaa
    15661 caataaagaa attgcagatg ccttgtactt gagtgacggc actgttcgca attacatcag
    15721 ccaattacta gaaaagctag atttacggga tcgcacgcaa ttggctatct atttttatca
    15781 gcatttgcaa taagtcttat ctaacacaaa aaaacctgaa tgatgctcct tcgcatcatt
    15841 caggtttttt tcacatcttc tattcaattg tcccgcaaac catttcctca cgattgaaag
    15901 aatcaaggat tcgcagggct gccaggattc gttggattgt tcggcgtgtt aggcggaaaa
    15961 ataggcgcct tgtctaacgt tttttctagt tttgattctt cacgatcgtc tcgttctaca
    16021 tcaggacttt cattcaattt gaattctttt tccatctcat ctaacttttt aaagtcattg
    16081 ccagattctt ttgctaattt cgctaattta tcttcggttt ctttagccat aaagtaacgc
    16141 cccccttttg atttcagctt agcatatcac actgaaaaaa caaaagaaaa cgctaaccaa
    16201 cgatcatttt tagctatttc tcatctttaa aatcaagcat tttttgccgg tataaaatcg
    16261 tagggagaac tgttttccat accaatcatc ggatctacta actggttatc gatcacttct
    16321 gctgtaagaa cgaaatcttt agaaacagca ggttctacct cttctttctt ttcttctcca
    16381 acaaatgaag aaaacagatc tggttccact gtttccatta cttggttttt cgtttcattc
    16441 gtcccactgt catcttctac aggtagcttt tcttttacaa cccgtggctc ttttgtatag
    16501 ttcccatctt caaatggatc atttccgacc aaagaatctt cataagctgc tagtttggtc
    16561 cggagagtga tattcatttt atcattatcc atgatccttg atttcaacgt agtcatccgg
    16621 cttgctgatt cattcgctac acaagtgaca taggcttgct cttcccctaa caaaatcaac
    16681 agttccttac tgcgagtcac tgccgtgtag agcaaattac gctgaagcat ccgctgatat
    16741 tgatggacca tgggcaaaat aaccatcttg aattcactgc cttgtgattt atggattgag
    16801 caacaataag aaagagtgat tttattccat tcgtttcttt tataagtgac ctcatttgca
    16861 tcaaactgga taactaattc atccacttta tcttcagaat cttttgccaa gatgataccc
    16921 gtgatttctc ccatatctcc attgaataca ttcaattcgg gagtattgac taattggagg
    16981 actttgtctc ctatccgata aacggtgtcg ttccaatgta cttccttttt tccaggtttc
    17041 cctggattga atatctcctg catcatttta tttaacgcat cgatccctgc aggtccccga
    17101 tacattggtg ctaatacttg gatatcttgt gcacggaatc ccttattttt cgctttttcc
    17161 acaattttag agatcaaggg ttcaatttga taagcatcac ttcggaaata ggaacgatct
    17221 ttttgattct ttgtgaaatc ttttggcaga cgtccttctt tgatctcatg ggcaagagga
    17281 ataatactag aaccatcacc ttgccgataa atctgattga gttccatttt gggaatttgt
    17341 tcgatttcca aaagatcatg gagtacttga ccaggaccga cagagggaag ctgatctttg
    17401 tctcctacta agataacttg catattcgtc ggaatcgctt tgaataatgt gttagccagc
    17461 catgtatcga ccatagacat ctcatcaaca atcaaaagtc cgccttctaa ttctttcgta
    17521 ctgactgaag gtgttttttc ccgtccattc agccccagaa gacgatggat cgtactggca
    17581 ggcagacctg tcgtttcgtt cattcgttta gctgctcggc cagttggtgc tgcaagtaga
    17641 ataggaaaaa tttcttgggt atattttgaa ggatccaatt ccagtccgtt caactctgca
    17701 aaaagattga cgattccgtt gatcacagta gttttccccg tacctggtcc tcctgttaaa
    17761 ataaatagag gggaacggat tgcttcagcg atagcttgtt cttgcgaagg tccatactgt
    17821 atgccgaata ttttttcgat acgccgaata ttcttctgca cttctttttc aggataatgg
    17881 atttcttttt tacgactgag aaggcgctgg atcgaattac cgatccccca ttcagaaaag
    17941 tacaaactgt tttcgtaaag attagtctct tcttgttgta tctttccttc ttcaacaagt
    18001 tggataatta cagtagcaac ctgatcaggc tggatttcaa ttgggcgact tgtttctaaa
    18061 atacggatcg tttgctgaag caatgtctcc gccggtacgt atgtatctcc tgtttgaact
    18121 gcatgctgaa aaatctgatg caagatagct gctcgaattc ttctcggtga atctgctgca
    18181 ataccgattt gttcggctag attatctgca cgtttgaacc cgattccttc tatgtcttcg
    18241 actagctggt acggattttc ttcgatgata tccaaagact cgttcttata ggcctggtag
    18301 atactgaaag ctaactgact accgaacccg taacgattca gtcccatgat cacttgatcc
    18361 atcccgtggt ttagccgaat cgtctcagca agcatttctc tttttggctt cgtcaaaccg
    18421 gagatttgtt ttaataccga aggatcagca atgatttttt cgatagcatc ttctcccagc
    18481 aattctacaa ttttttctgc tgttttcttc ccgattcctg ggaatttttc gctggaaaga
    18541 tacgctacga tcccatttgc agttgttggc ttttcttgct catatctttc gagtttaagc
    18601 tgccttccgt atttaggatg atccactaat tctccataaa aacgataagt ctcattttct
    18661 tgaatatcgc cgaaacttcc ggtgactaca atctcttttt cacggtaatc agctgtggtt
    18721 tcactgatgt gtaccaaaag cactttgtaa aaattactag gattttgaaa aaaaatcgcg
    18781 gcaactgtac caacgaaata aggcatttct tctggctgca tcggaagccg cctccttctt
    18841 ctatagtaaa ggttctggtg ctgtttcagt tcctaaaaaa tctgtttggt tccatacttt
    18901 cagttcatat ggcaataatc tgcaaggctc ttcagtctct aaaatcgtca aactactatt
    18961 gaacaatcca cccatttcac gcaactcact tagctctttt cctgtcatcc attgaatggc
    19021 tgcagttaaa gaagctccat gtcctacgaa taaaagtggg ccatctttat gtcgggcaac
    19081 cgcatctgct acaacagttt cgatacgtgt gattgccgat tcaatcgttt ctccgtgaaa
    19141 aggagaagga tcatacagat ctaaatgatt acgcaaattc ggcagattat ccggatattt
    19201 ctggtacatt tcatcgatcg actgaccttc taaagtaccc agccctagtt ctcttagctt
    19261 atctgtataa aaaatctcta ccggacgagc aagctctttg ttgatacctt ctgctgtatc
    19321 acgtgctcgc tttgctgtac tggaataaat tgctttaaaa ggaacatatt ttaccttttt
    19381 cccaaatgct ttgatttctt cgtaactttg tggcaaaagc ggagaatcgc cattgctccc
    19441 ttgaaaacgt ctttcctgat tccattgtgt cttcccatgt cgggtaaaat atagttccat
    19501 acgcagcaaa ctcctgtctt tcttttattt tactctataa taaactgtct aatccagctt
    19561 gtcattttca tagtgatgcg tcaattctaa attagggaat ccttgctggc gaagtgcttc
    19621 atacactatc attgcagctg tattcgataa attcaatgag cggacatgct catcattcat
    19681 agggatgcgt aaacactttt cttcgttttc acgcataaat gtttctggca aaccggttgt
    19741 ttcttttcca aataaaaaga agtgctcttc tccatcagca aaattttcgt cactatatac
    19801 tttatgggca aattttgaga tcaaatgtag tttacgatta cctaaaaatg ttaaaaaagc
    19861 ttctaaatta ctgtggtaag taatatttac atcattccaa taatccaacc ctgcccgttt
    19921 tagatgtttg tcatcagttg aaaaacctag tggctcgatt aaatgaagcg gagtattcgt
    19981 ggctgcacac gtacgggcaa tatttccagt gtttgcaggg attaatggtt caaataaaac
    20041 gatatgattc atcaggatca ttcctctcat atttatgttc gtttctattt tctgacaatt
    20101 tgatttttgc ataaaaaaaa acgtatcaac tcggtgtcag tcactgcgag tcgttacgtt
    20161 agtgaagctc ttctcactaa cttttatcag aacaggaacc tgataaaaac caatgaaaaa
    20221 acaacttgtt gctaatataa caccctcaac ttttttttgc aacctctact cattaaaaac
    20281 tgctagaaaa acgttttctc ttagttcctt ttaataattc tatgtcaatt gtaatcttct
    20341 tcaaaggatt cttatataag tctgctttga ttcgctcttc tacctgccat tctaaaggtg
    20401 acatggccaa aagatctttt tgattttcct ctggtatctc aaattcatag gttacacgct
    20461 gacgttcagt taatttcatt gcttcagcaa acttggctac tacacgttca ttggaataac
    20521 tctgtttttc aggttgattc ggataaaaag cctgacgtag ttcctttaaa taattttctt
    20581 gtggcaccac cttcaacact tctccatctt ctgagagtac acgttggaat tcttggtaat
    20641 gagaaggcga aaagatattt aaaaccgcgt tgaatgtttg atcagcaaac ggcaaattgg
    20701 tcaaatcagc tacacaccaa aatgcttcgg cagattgttc tgtagctgcg tagactcctt
    20761 cttttgaaat atcaaatcca acagcatttt gaatcttttt caattttgaa agttcattca
    20821 agaagctacc ttctccgcag ccaacatcta atagcgagac ttcttcaggt aaatgctgcg
    20881 caatagtctc aataacgggc tgatacatgc cagattggat cattctacgc cttggaacga
    20941 acattccttg atcgtattct gttttaaccg catgggataa aaaataaagt gttccttttt
    21001 tggaaaggtc aaaacggtga ttatttttgc atatcaagcc attatcagac ttttttactt
    21061 cttcttggca aaccggacag cggaacagct gatgattttc tgccaaaaac tggcgtcctc
    21121 ggtcaatttt ttttaacata gttccccctc atttctgttt tatcttaccg ttagatgctc
    21181 actttggcaa ctgctaaaaa tatgatacaa tgcctagaag aaagtgaaag ataaaggaga
    21241 agattagatg atcaaagaat tttgcgctga aaactacaca tctattcctc tagcaattgc
    21301 tagaggagcc gatcggatcg aattgtgtga caacttagcg gtcggtggca caaccccgag
    21361 tactggtgtg atcgaagaaa cgttaagtta tgctggggaa aaagatatcc ccgtaatgac
    21421 gattattcgt cctagagggg gcgacttcgt ctataacgat atcgaattga aaatcatgca
    21481 tactgatttg attgaagcaa agaaattagg gacggatggc gtagtatttg gttgtcttac
    21541 tccttcaggc tggctagacg aagaggcctt agaactattg atcgaaacag cagaagggtt
    21601 gcagatcacc ttccatatgg cttttgacag tattcctgaa gatcggcaat tcgaagcaat
    21661 cgattggctg gtagaacatg gtgttgaccg tatcttgact catggcggtc ctgctgacaa
    21721 aactattgta gaaaatttcc cacggcttaa agaattgatt gcctatacag atggccgctt
    21781 aatcattttg cctggtggtg gtgtcactgc agagaatgca gcacatgtag ctaaagaatt
    21841 gaacgtaagc gaagtacacg gaaccaaaat cgtggatctt cagttataat cttataaaca
    21901 aaaaaacatt tgcaacaggc ataatgctta ttgcaaatgt ttttgttttt ttacataaaa
    21961 tagcccataa ataaagaagc aatattgaaa taaatcagta cactcgtcaa atcgctcaaa
    22021 gtcgtgataa agggaccgct tgctactgct ggatcaaatc ctagacgatc cattagcatc
    22081 gggattaaac tacctgcaag attcgccact gtgattgcaa atagcatagc catccctatg
    22141 acaaacccca agataaaatt atgtttccaa aaacctacga ttagaaaaat cgtgatcccc
    22201 gtaacagctc ctgtgacaac gccggtcaag acttcagcca aaacaagtcg aacaaatcca
    22261 tgatctttct catcagaaat cgcaagtcga cgaacagcca cagctagcga ctgggttcca
    22321 gcattaccgg cagttcctgt aatcagcgaa atgaagactg ctaaaatact cgcttcactt
    22381 accaagtctt catagtgact gattaaagaa gcggtcgaca tacctaaaaa caacagagtg
    22441 atcagccaag gcaaacgtcg agacgctgcc tttactggat tttcattaat ttcttccaca
    22501 ttgaccccgg caagtcctga ataatcgcta gcagcttcat catcaataac gtcgatgata
    22561 tcatcgactg tgacgattcc taacaaatga tcatcataat cggtaacagg aagagctaag
    22621 aagtcataat ctctaaatgt ttgtgccaca tcttcttggt cgtcgcctac atgaactgaa
    22681 agcactcgtt cgctcatcag atcagaaatc attgcgtcgt catcattcac gattaggtcg
    22741 cgcagagaaa taacccctac tagctgacct tcttgattaa cgacatagat gtaatagatt
    22801 gtttcagcta catctgcttc gttcttcagc acgtacatcg cagagcgaac agtttgattg
    22861 gctacgatcg aaacaaattc agttgtcatg atcgcgccag ccgtttcatc ctcgtaatgg
    22921 agaagttcct tgatttcact ggcatcgtct gccgacatca aacttaaata tttagcaatt
    22981 tgctttttat ccagcgtatt aagcaagtca acagcattat ctgtatacat ctcagaaagc
    23041 atatcagctg catagctagg acgcatttca tctaggtatt ctttcatatg ttcgttgtct
    23101 tcttcaatca catcgaacat atccgctaat tctttaggcg acagatagga atagatcagc
    23161 tgcctgttct cttctgtaag agattggtag aattgcccct gctcatagat atgcatctct
    23221 aaaaaactgt ttctgaatgc ctgaatatta ttttccctta aatcttgagt caactgggca
    23281 aatctttcct ctaattcttg attttcatcc aaaccaatct cacctcgcct tattcttcaa
    23341 accgtctcac aactgcctcc atgtctgcgg ctaaaggctg cttgaactcc agcaactctt
    23401 ctgtaaatgg atggtaaaaa cgcagatcat aacaatgaag ggcttgtctt tttattccca
    23461 gatccattcg cccgccatac atatcatcac cgagcagggc acagccaatt gcggaaaaat
    23521 gaactcttat ttgatgtgtc cttcctgtat gcagctggat atcaactaac gcgatttctg
    23581 ttagtcgctt ctctatccag tactcagtct gtgccgaacg tccttcatca gagaccattc
    23641 gcttcaatag cgaagaataa tctcgggcga tcggcaaatc gatatgtcca tgtgccgata
    23701 aatctgaaag ccttccactg actaaggcct gatatttttt cacaaacttc ttttcacgaa
    23761 gttgcatatc taattttgcg tgcgcaaatc catgctttgc aaaaagcatc aagccagatg
    23821 tatcccgatc tagtcgcgtc accacatgga ttacttggtt ttcgtaacct tgttttttat
    23881 aataagcctt tacacgatta gccattgtcc catttggatg gtattgggca ggaatagacg
    23941 aaacgcctgc tggtttattt actaccagaa cgtgttcatc ttcatacacg atatcgatcg
    24001 gtgtttcatc caaaagtacg gtttcatgtt ctccttcagc aggaatcaca atggttacct
    24061 tatcatcctt agctagagaa aacaagacat tttctacttg atcattaact ttgatttgac
    24121 cgccttgaaa ttttattttc gctaacagac cctttgagat ccctttttct tttaaaaaat
    24181 attttacttg ctgagaatga tctttctcat aatcccatgt aaactgcata tcaatcctca
    24241 ctaataaagg catctttcac acgatgccaa aaatgcatat gtcgataaga agcaaaatgg
    24301 atccgttctt ctgctattcg ataataaatt gagcggatat ttgcttttgc gacattcaat
    24361 tgatcaaccg ttacaagata atcttctgta ttttccaatt ttatttctag ccattcatca
    24421 tgggcaatca cgatcggaga acccaaagta cgaaacactc gattattcag tgaagcaatc
    24481 tctgccaact gaaaggcatt gatactagga tggatcacag caccgccgac acttttatta
    24541 tatgctgtcg aaccagtagg cgtagaaatc gtcaatccat ctcctctaaa actttcaaac
    24601 agctcgtttt tgatgtatac atcggctacc atcgtacgat tggcccgttt aatcgttgat
    24661 tcattcagtg ctaaaaaatg ctggtccggt gtttcatcca aataactgat ccgaacatcc
    24721 agcaagggat aactaacact ttgttcccgg ttcgttcgta agctgtctac tagttcttcc
    24781 agctcgtaat cccgccaatc cgtgtaaaac cctaaatgac ctgtatgcac gcccaagaaa
    24841 cgcacatcat tcagacaatg actgtagtga tggaacgccg acaataatgt gccgtctccg
    24901 ccgactgaga tgaccaattc tggctcatga tcatctcgtt gaatccctgc ttgttctaaa
    24961 agtgcgttca aacgttctgt cacttctata gtctttgctt ctttattatg aacgatcgct
    25021 actttcatcc cctgcctcct ctctttcgct ttctttcttc aaaagcgttc gatagtagac
    25081 atcatctgat tttcctcttc catgggagaa taaccgctgt gcttcttgga tttcttcacg
    25141 gatactggac atctcttcat ccaactgata ggcagcttct gccgcacgct gaagtcgttc
    25201 actcatttct tctggaaaaa cgccttgata tttataattc agcgaatgct caatcgttgc
    25261 ccagaaattc atcgcaagag tccgaatctg gatttccgcc aaaattttct tctcaccgct
    25321 gatcagctgt acaggatact cgatcaccag atgataagaa cggtagccac ttgctttttt
    25381 atttgtgatg taatctcgtt cttggagaac tttcatgtct tttcttttac ggatgatctc
    25441 aacaacttga tggatatctt ctacaaactg acacatgatc cttaacccgg caatatcaga
    25501 catttcttct tctaaacgtc ccattggaat atgccgtaat tgtgattttg ttacaatact
    25561 atcgactggc ttgactctcc cagtgacaaa ttcaatcggg acatgtcgat tttgttcacg
    25621 gaactgtttg cggatccccc gcagtttcac tttcaattct gatacagttt gttcatatgg
    25681 tgctaaaaat gcttcccaat ctcgttccat agctgatacc tctacttcat taacattcat
    25741 tgtttattct accatatatg caaagttttt tcgtactctt gatttatgca ttgaccaaaa
    25801 aaagaaaatt aggcaaaata gacaaaaagg agtcgatcaa atgacacaaa tgcttgaaat
    25861 cgaatataaa tctatgctga caaagcaaga gtacgagata ttcatcgctc actatcaatt
    25921 aacagataaa gatttccgtg tacagactaa tatttatttc gataccgttg atgaacagct
    25981 gaagaaacaa aattgtggcc tacggatccg tttcgttggt catcaagctg aatatacatt
    26041 aaaaacacct gctgaaaaag ggcgtctcga aacaacggat acctttataa cagaagaagc
    26101 agaagcattt atcttcgaaa aacgtctgcc tcgttcaggc gcagtttttc aaaaactttc
    26161 tgatttgaat atcgatcctg cttcattaat ccaaaccgga aagcttacaa caaaacgagc
    26221 tgaattccct attgaagaag gacttttagc tattgatgaa agctggggag acaatctaca
    26281 cgattttgaa ctggaactag aggtagggga tgcatctgtg ggaaaaattg cttttataaa
    26341 ttttttaaaa cgtttttctt taccttatcg tcctgctaag aacaagatcc aacgtatgct
    26401 tgaagctaaa aattagaaac ctttcatttt cacaaggcac ttgagttcat ttttttactt
    26461 ttttctgtta agttatagga tgtgaaagaa tctagtcact agttgtatag aatctatttt
    26521 gggggagatt catatgatcg aaatctattt atttgttaat cctttagggg aatactgttt
    26581 cgaaatggaa caacagttat tacaatttat cgaagaagag tacgggacac catcaaaaga
    26641 aaagatgcaa tttcgctttc ttcctttggt caatttgcag acaattgggg atgtcatgca
    26701 acaaaaagga atttctcaga acaatcttga ggaacgtaat cacttattct caatgactta
    26761 ttctgcagca ttggactgca aagcggcgca gtttcaagga aagaaaaaag gccgccgttt
    26821 tttgatcaaa ttgcaagaag cagtcggctg taatgacata ccatattctc aagaacttgc
    26881 tgaatccata tttgaagaaa tcggcggcga tttgaatatg tttaaagaag atcgtcagtc
    26941 tgacttcgtt aaagaagtat tcttttctga tcaaaaaatt gctcgggaaa tgggcatcac
    27001 aaaacaccct tctgcagtcg tttacaatta tgcctgcgac cgtgattttg gtgttttagt
    27061 agaagtaaaa ggtacaagta gcctttggag aatcacagag ctgtgtaaaa caacagataa
    27121 ctcatatcaa gtttttaatg gtgaacattt acatacaaat gattctcagt tccattcaag
    27181 aactagccat ttacacttgc tttctaatta a
//
LOCUS       DACDYM010000033        26598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDYM010000033 DACDYM010000000
VERSION     DACDYM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.033"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWM59_001418"
     CDS             complement(99..1229)
                     /locus_tag="IWM59_001418"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9979459.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWM59_001419"
     CDS             complement(1878..3080)
                     /locus_tag="IWM59_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9979460.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWM59_001420"
     CDS             complement(3140..3343)
                     /locus_tag="IWM59_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9979461.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWM59_001421"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWM59_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWM59_001422"
     CDS             4880..5692
                     /locus_tag="IWM59_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9979462.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IWM59_001423"
     CDS             complement(6716..6868)
                     /locus_tag="IWM59_001423"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979463.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IWM59_001424"
     CDS             complement(7059..7937)
                     /locus_tag="IWM59_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9979464.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IWM59_001425"
     CDS             complement(7976..9094)
                     /locus_tag="IWM59_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9979465.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IWM59_001426"
     CDS             complement(9091..11190)
                     /locus_tag="IWM59_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9979466.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IWM59_001427"
     CDS             complement(11241..12062)
                     /locus_tag="IWM59_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9979467.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IWM59_001428"
     CDS             complement(12059..12868)
                     /locus_tag="IWM59_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9979468.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IWM59_001429"
     CDS             complement(12889..13383)
                     /locus_tag="IWM59_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9979469.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IWM59_001430"
     CDS             complement(13395..13883)
                     /locus_tag="IWM59_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9979470.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IWM59_001431"
     CDS             complement(13960..16656)
                     /locus_tag="IWM59_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9979471.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IWM59_001432"
     CDS             complement(17084..18157)
                     /locus_tag="IWM59_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9979472.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IWM59_001433"
     CDS             complement(18154..18981)
                     /locus_tag="IWM59_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979473.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IWM59_001434"
     CDS             complement(18982..19788)
                     /locus_tag="IWM59_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979474.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IWM59_001435"
     CDS             complement(19800..20885)
                     /locus_tag="IWM59_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979475.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IWM59_001436"
     CDS             complement(20902..21444)
                     /locus_tag="IWM59_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9979476.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IWM59_001437"
     CDS             21702..22691
                     /locus_tag="IWM59_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9979477.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IWM59_001438"
     CDS             22688..23425
                     /locus_tag="IWM59_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9979478.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IWM59_001439"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IWM59_001439"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9979479.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IWM59_001440"
     CDS             23989..24621
                     /locus_tag="IWM59_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9979480.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IWM59_001441"
     CDS             24706..25353
                     /locus_tag="IWM59_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9979481.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IWM59_001442"
     CDS             25358..26341
                     /locus_tag="IWM59_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9979482.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8140 a         5616 c         4509 g         8333 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcagcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacata
//
LOCUS       DACDYM010000034        25361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDYM010000034 DACDYM010000000
VERSION     DACDYM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.034"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(135..512)
                     /locus_tag="IWM59_001443"
     CDS             complement(135..512)
                     /locus_tag="IWM59_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979483.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            768..1496
                     /locus_tag="IWM59_001444"
     CDS             768..1496
                     /locus_tag="IWM59_001444"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9979484.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1496..1750
                     /gene="purS"
                     /locus_tag="IWM59_001445"
     CDS             1496..1750
                     /gene="purS"
                     /locus_tag="IWM59_001445"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9979485.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1752..2423
                     /gene="purQ"
                     /locus_tag="IWM59_001446"
     CDS             1752..2423
                     /gene="purQ"
                     /locus_tag="IWM59_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9979486.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2424..4646
                     /gene="purL"
                     /locus_tag="IWM59_001447"
     CDS             2424..4646
                     /gene="purL"
                     /locus_tag="IWM59_001447"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9979487.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4631..6070
                     /locus_tag="IWM59_001448"
     CDS             4631..6070
                     /locus_tag="IWM59_001448"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9979488.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6102..7145
                     /locus_tag="IWM59_001449"
     CDS             6102..7145
                     /locus_tag="IWM59_001449"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9979489.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7142..7720
                     /gene="purN"
                     /locus_tag="IWM59_001450"
     CDS             7142..7720
                     /gene="purN"
                     /locus_tag="IWM59_001450"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9979490.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7717..9252
                     /gene="purH"
                     /locus_tag="IWM59_001451"
     CDS             7717..9252
                     /gene="purH"
                     /locus_tag="IWM59_001451"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9979491.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9268..10542
                     /gene="purD"
                     /locus_tag="IWM59_001452"
     CDS             9268..10542
                     /gene="purD"
                     /locus_tag="IWM59_001452"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9979492.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10664..11578
                     /locus_tag="IWM59_001453"
     CDS             10664..11578
                     /locus_tag="IWM59_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9979493.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11711..13276)
                     /locus_tag="IWM59_001454"
     CDS             complement(11711..13276)
                     /locus_tag="IWM59_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9979494.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13269..14720)
                     /locus_tag="IWM59_001455"
     CDS             complement(13269..14720)
                     /locus_tag="IWM59_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9979495.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14734..15750)
                     /locus_tag="IWM59_001456"
     CDS             complement(14734..15750)
                     /locus_tag="IWM59_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9979496.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15970..17058
                     /locus_tag="IWM59_001457"
     CDS             15970..17058
                     /locus_tag="IWM59_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9979497.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17112..18638
                     /locus_tag="IWM59_001458"
     CDS             17112..18638
                     /locus_tag="IWM59_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9979498.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18640..19845
                     /locus_tag="IWM59_001459"
     CDS             18640..19845
                     /locus_tag="IWM59_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9979499.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20102..21079
                     /gene="guaC"
                     /locus_tag="IWM59_001460"
     CDS             20102..21079
                     /gene="guaC"
                     /locus_tag="IWM59_001460"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9979500.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21343..22767
                     /locus_tag="IWM59_001461"
     CDS             21343..22767
                     /locus_tag="IWM59_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9979501.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23228..24337
                     /gene="pdhA"
                     /locus_tag="IWM59_001462"
     CDS             23228..24337
                     /gene="pdhA"
                     /locus_tag="IWM59_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9979502.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24340..25317
                     /locus_tag="IWM59_001463"
     CDS             24340..25317
                     /locus_tag="IWM59_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9979503.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8204 a         4316 c         5696 g         7145 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
      661 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca
      721 aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag
      781 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
      841 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
      901 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
      961 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
     1021 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
     1081 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
     1141 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc
     1201 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
     1261 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
     1321 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
     1381 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
     1441 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
     1501 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
     1561 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
     1621 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
     1681 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
     1741 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
     1801 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
     1861 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
     1921 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
     1981 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
     2041 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
     2101 tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
     2161 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
     2221 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
     2281 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
     2341 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
     2401 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
     2461 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
     2521 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
     2581 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
     2641 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
     2701 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
     2761 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
     2821 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
     2881 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
     2941 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
     3001 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
     3061 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
     3121 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
     3181 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
     3241 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
     3301 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
     3361 cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
     3421 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
     3481 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
     3541 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
     3601 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
     3661 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
     3721 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
     3781 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
     3841 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
     3901 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
     3961 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
     4021 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
     4081 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
     4141 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
     4201 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
     4261 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
     4321 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
     4381 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
     4441 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
     4501 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
     4561 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
     4621 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
     4681 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
     4741 aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
     4801 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
     4861 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
     4921 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
     4981 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
     5041 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
     5101 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
     5161 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
     5221 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
     5281 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
     5341 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
     5401 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
     5461 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
     5521 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
     5581 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
     5641 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
     5701 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
     5761 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
     5821 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
     5881 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
     5941 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
     6001 tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
     6061 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
     6121 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
     6181 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
     6241 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
     6301 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
     6361 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
     6421 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
     6481 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
     6541 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
     6601 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
     6661 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
     6721 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
     6781 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
     6841 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
     6901 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
     6961 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
     7021 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
     7081 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
     7141 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
     7201 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
     7261 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
     7321 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
     7381 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
     7441 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
     7501 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
     7561 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
     7621 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
     7681 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
     7741 tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
     7801 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
     7861 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
     7921 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
     7981 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
     8041 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
     8101 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
     8161 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
     8221 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
     8281 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
     8341 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
     8401 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
     8461 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
     8521 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
     8581 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
     8641 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
     8701 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
     8761 ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
     8821 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
     8881 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct
     8941 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
     9001 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
     9061 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
     9121 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
     9181 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
     9241 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
     9301 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
     9361 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
     9421 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
     9481 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
     9541 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
     9601 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
     9661 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
     9721 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
     9781 aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga
     9841 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
     9901 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
     9961 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
    10021 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
    10081 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
    10141 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
    10201 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
    10261 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
    10321 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
    10381 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
    10441 aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
    10501 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
    10561 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
    10621 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
    10681 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
    10741 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    10801 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    10861 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    10921 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    10981 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    11041 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    11101 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    11161 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    11221 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    11281 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    11341 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    11401 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    11461 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    11521 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    11581 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    11641 tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg
    11701 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    11761 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    11821 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    11881 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    11941 tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat
    12001 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    12061 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    12121 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    12181 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    12241 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    12301 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    12361 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    12421 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    12481 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    12541 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    12601 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    12661 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    12721 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    12781 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    12841 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    12901 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    12961 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    13021 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    13081 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    13141 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    13201 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    13261 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    13321 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    13381 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    13441 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    13501 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata
    13561 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    13621 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    13681 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    13741 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    13801 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    13861 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    13921 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    13981 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    14041 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    14101 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    14161 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    14221 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    14281 acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata
    14341 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    14401 aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta
    14461 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    14521 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    14581 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    14641 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    14701 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    14761 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    14821 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    14881 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    14941 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    15001 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    15061 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    15121 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    15181 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    15241 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    15301 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    15361 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    15421 tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    15481 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    15541 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    15601 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    15661 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    15721 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    15781 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    15841 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    15901 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    15961 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    16021 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    16081 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    16141 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    16201 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    16261 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    16321 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    16381 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    16441 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    16501 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    16561 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    16621 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    16681 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    16741 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    16801 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    16861 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    16921 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    16981 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    17041 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    17101 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    17161 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    17221 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    17281 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    17341 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    17401 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    17461 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    17521 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    17581 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    17641 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    17701 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    17761 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    17821 gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    17881 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    17941 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    18001 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    18061 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    18121 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    18181 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    18241 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    18301 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    18361 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    18421 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    18481 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    18541 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    18601 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    18661 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    18721 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    18781 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    18841 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    18901 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    18961 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    19021 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    19081 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    19141 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    19201 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    19261 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    19321 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    19381 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    19441 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    19501 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    19561 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    19621 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    19681 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggtatg
    19741 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    19801 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    19861 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    19921 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    19981 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    20041 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    20101 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    20161 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    20221 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    20281 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    20341 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    20401 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    20461 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    20521 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    20581 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    20641 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    20701 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    20761 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    20821 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    20881 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    20941 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    21001 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    21061 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    21121 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    21181 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    21241 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    21301 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    21361 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    21421 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    21481 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    21541 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    21601 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    21661 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    21721 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    21781 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    21841 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    21901 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    21961 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    22021 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    22081 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    22141 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    22201 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    22261 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    22321 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    22381 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    22441 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    22501 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    22561 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    22621 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    22681 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    22741 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
    22801 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
    22861 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
    22921 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
    22981 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
    23041 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
    23101 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
    23161 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
    23221 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
    23281 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
    23341 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
    23401 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
    23461 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
    23521 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
    23581 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
    23641 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
    23701 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
    23761 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
    23821 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
    23881 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
    23941 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
    24001 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
    24061 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
    24121 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
    24181 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
    24241 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
    24301 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
    24361 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
    24421 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
    24481 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
    24541 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
    24601 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
    24661 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
    24721 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
    24781 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
    24841 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
    24901 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
    24961 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
    25021 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
    25081 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
    25141 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
    25201 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
    25261 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
    25321 aagaagggac gaaacaaagt catggcttat caatttaaat t
//
LOCUS       DACDYM010000035        25221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDYM010000035 DACDYM010000000
VERSION     DACDYM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.035"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..485)
                     /locus_tag="IWM59_001464"
     CDS             complement(<1..485)
                     /locus_tag="IWM59_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9979504.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     C"
     gene            793..1599
                     /locus_tag="IWM59_001465"
     CDS             793..1599
                     /locus_tag="IWM59_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979505.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1611..2831
                     /locus_tag="IWM59_001466"
     CDS             1611..2831
                     /locus_tag="IWM59_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979506.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2821..4407
                     /locus_tag="IWM59_001467"
     CDS             2821..4407
                     /locus_tag="IWM59_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9979507.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            4446..6584
                     /locus_tag="IWM59_001468"
     CDS             4446..6584
                     /locus_tag="IWM59_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9979508.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6872..8263
                     /locus_tag="IWM59_001469"
     CDS             6872..8263
                     /locus_tag="IWM59_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9979509.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            8322..9131
                     /locus_tag="IWM59_001470"
     CDS             8322..9131
                     /locus_tag="IWM59_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9979510.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            9103..10092
                     /locus_tag="IWM59_001471"
     CDS             9103..10092
                     /locus_tag="IWM59_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9979511.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            10206..11201
                     /locus_tag="IWM59_001472"
     CDS             10206..11201
                     /locus_tag="IWM59_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9979512.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            11205..12347
                     /locus_tag="IWM59_001473"
     CDS             11205..12347
                     /locus_tag="IWM59_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAP9979513.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            12344..13771
                     /locus_tag="IWM59_001474"
     CDS             12344..13771
                     /locus_tag="IWM59_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9979514.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(13838..15127)
                     /locus_tag="IWM59_001475"
                     /pseudo
     CDS             complement(13838..15127)
                     /locus_tag="IWM59_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15307..15717)
                     /gene="tagD"
                     /locus_tag="IWM59_001476"
     CDS             complement(15307..15717)
                     /gene="tagD"
                     /locus_tag="IWM59_001476"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAP9979515.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            15843..17234
                     /locus_tag="IWM59_001477"
     CDS             15843..17234
                     /locus_tag="IWM59_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAP9979516.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            17367..18314
                     /locus_tag="IWM59_001478"
     CDS             17367..18314
                     /locus_tag="IWM59_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9979517.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(18354..19514)
                     /locus_tag="IWM59_001479"
     CDS             complement(18354..19514)
                     /locus_tag="IWM59_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9979518.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            19679..20854
                     /gene="mltG"
                     /locus_tag="IWM59_001480"
     CDS             19679..20854
                     /gene="mltG"
                     /locus_tag="IWM59_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9979519.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            20990..21469
                     /gene="greA"
                     /locus_tag="IWM59_001481"
     CDS             20990..21469
                     /gene="greA"
                     /locus_tag="IWM59_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9979520.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            21637..22368
                     /locus_tag="IWM59_001482"
     CDS             21637..22368
                     /locus_tag="IWM59_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9979521.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            22365..23432
                     /locus_tag="IWM59_001483"
     CDS             22365..23432
                     /locus_tag="IWM59_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9979522.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            23439..24071
                     /locus_tag="IWM59_001484"
     CDS             23439..24071
                     /locus_tag="IWM59_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9979523.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            24211..24876
                     /locus_tag="IWM59_001485"
     CDS             24211..24876
                     /locus_tag="IWM59_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9979524.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            24904..25197
                     /locus_tag="IWM59_001486"
     CDS             24904..25197
                     /locus_tag="IWM59_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9979525.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8570 a         3775 c         4888 g         7988 t
ORIGIN      
        1 atacacaaca ccgtcggaag gaagttcata cgtggctggt aatgcttcgt tgcttcacgc
       61 aagatccgtg tcaccgtccc atccgagacg aagtacttct tagcgattag tttacgtgat
      121 tgattttcgt acagttccat gagaatcgcc tgcttcagtt cctttgttag ttgatgatgt
      181 tcatcaacta agtccgtttt cgcagaaaag gtccttccac aatcatggca gagaaaacgg
      241 gtacgtttta attctaaaat agttaacttt tctttgactt tcaacatctg agtgcgtgtc
      301 gtataggaac cgttgcggat aatacgaacc gatcgacagt gttcacaata ggaagaagag
      361 tcatccaatt ttcctttaat aaactgcgct tgaacgcctt tatattcttt ttcaatcagc
      421 cattcttcat caaaaaataa atgtttatct gttaatccga gtaattttct agtatgatta
      481 tccattgaga gactcctttg gtaggatgac gttttggtcg actaaatttt accaaaagtg
      541 agtctctttt tgagtttaaa cgaagaaaaa gtgttagtag aaaatcgcct tcgattttcc
      601 accaacacaa aatattatag aacccctttt ttggtttatt gcaattgtct agcatcttct
      661 gtaaaataga aacgttagag aaaatcttgt atcatagaaa attaagaaag aaataagaac
      721 gcagctcggt accaaaaata tggtaaaatg gcacttgact gtaattcact gaaatcgctt
      781 ggaggaacac aaatgtttaa agatttgtat ttatttatga aagacattta tcagaataga
      841 aaattactgc tacagttctc gatcaatgac ttcaaatcaa gatatgcagg atcgttttta
      901 ggaatcttat gggcgtttat caatccgcta tttactgtat tgatttattg gctggtattt
      961 ggtgttggac tgaaagcaca aatgatcgat gcaaaatacc catttatcgt atatttagtt
     1021 accgggatga ttccctggtt cttcttctca gatgcatttg catcaactac ggttgttttt
     1081 cgggaatata cttatttagt aaagaaagtc gtattcaata ttcgtatctt gcccacgacg
     1141 aaaatcctat ccaatctgta cacccattta ttcttcattc tgattggcat agctgtcgcg
     1201 cttataaatg gtatatatcc ttcagcaatg tctttacagc tgatttatta tttattttgc
     1261 atgtgtgctt tcttgacagg attgacttgg ctgacggcat cgatccaacc atttttacct
     1321 gatatcatgc aattaattac gatcgtgctg cagttgatca tgtggacatt accgatatta
     1381 tggagcccta atcagtttga ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac
     1441 tatgttgttc aaggttatcg tgagtcattt ttaagtgaag cttggttctg ggagcatgga
     1501 acatatactt tgtatttctg ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg
     1561 ttccgtagat tgaaaccaca tttttcagat gtgctgtaag gagaatagct atgacagaat
     1621 atgcaattag actaaaagat atcacaaaat cttataatat gtacgcaaaa ccttcggatc
     1681 gatttcgtga agccctcaat ccttttaaga aatcctatca tgatgtattt tatgcgttga
     1741 aaaacgtgaa tgtagaaata aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat
     1801 cgggaaaatc tacaatgttg aaaatcatta cgggagtact gacaccaaca tccggcacga
     1861 tggaaatcga aggaaaaatc tcagctttgc tagaactagg atcaggattc aatcctgagt
     1921 attctggcta tgaaaatatc tatttaaatg gaatggttct aggattttca agagaagaag
     1981 tcgatgaaat ggtcgatgat atcattgaat ttgctgatat tggcgaccat atttatcaac
     2041 ctgtaaaaac ctattcaagt gggatgttcg ttcgtctagc ttttgctgta gcgatcaatg
     2101 tgaatcctga tattttgatt gtggatgaag cattggcagt aggagatctt gaattccaac
     2161 tgaaatgtat ggaaaaattt acagaaatta agaactcggg aaaaacgatt ttattcgttt
     2221 ctcatgatat tagttcgatt cgccgttttt gtgatcgttc ctattggttg aaaaatggag
     2281 aagttgtcga atatggtgat acgatggaag taacgacgaa ttacgaaaac tttttgaaga
     2341 aaaaatcagt taaaacagta gatcgaaata aaaaccaagt agaacgcttc gcagctccag
     2401 atatcgtaga cgtgacgaaa gccgaattgt tggataaaaa taaaaatcca gtggatatgc
     2461 tggaacaagg agaaaaacta tacgttaaag taacctatga agtaaaagac gatacaatca
     2521 aaaatccagt tttaggaatt gctctgcgta cagtcgacaa tttttatgtg tgcggactca
     2581 atactctact agatggtatc aagatccctt ggaaaaaagg tgagaatatc ttttatctgg
     2641 agtatgaaaa gttaggtttg ttagctggtg aatattattt tgacgttgcg gtctttgaag
     2701 aaaatgcaac ggttcctttg gtatacaaga caaaatacat gaacttgttt gtcagcggtt
     2761 cttatatcgg agaaggcatc gtggtcttag accataaatg ggaagaaggg actcatagca
     2821 atgaaatatg attttgaaat ggacctagat gaacaaagca gtgtgggaaa aattgccgca
     2881 cagatcaaac caggaagtaa agttttagag tttggtcccg gaaacggtcg attaacgaaa
     2941 catttgatcg gagcaaaaca atgtgaagtg agcattgttg agctagataa ggaattattc
     3001 gattttgtca gcgaatttgc ccaagatgga ttttatggag atatcgaaag ttttgaatgg
     3061 gcaaactatt atgctggaca gacatttgat tacgtcttgt ttgcggatgt tttagaacat
     3121 ttagtagatc ctggaaaaac attgaagaaa gtcagagaat ttttgaatga agaaggagag
     3181 atcctgatta cattccctaa cttagcacat aattctgtca tgatcgattt gttcaataat
     3241 caattgcctt gggcttctta tggtctccta gatgaaaccc ataattcttt ttatactcat
     3301 gatggtttcc aaaaagtttt tgaaaaagcc ggattattca ttaatattga agattacttg
     3361 tacttggctg taggagatac agaattaaaa tcaacttatg aagagttgcc ggaagctgta
     3421 cgctatgatt tcaaaatgcg accatttggt gaagtctacc aatacttttt ctcattgatg
     3481 aaacatccag tagtacaaac ttccattgca cagccacaaa actctaatta tgtaaaagta
     3541 ctagaagtaa cccagcaaac gaaacaagat gaaacaatcc aacaaatacc attcaataat
     3601 ttcacagggg aaaatgagac actaagtttc cccgtgtcag aagcaacaga aagaatggtg
     3661 tttcgcttcg cacagcaacc tagctttatc gaattcagtg cagaagctgc tggagaaaaa
     3721 ctcatattta tcgattcaaa tgctgtagtc aaaacagtaa atgattgtta tttgtttgat
     3781 gggaaagaaa taccagagtt cgctctaacg gatatttctg ggaaagaagt gactatccat
     3841 tgtcactatc gctttatcgg ggaattgaca ccaacaatga aagaattgct ggaaacaatt
     3901 cgtcctatgg ctgaagtcac aaagcagctt tctgagaaaa atgatgaatt agaaagagaa
     3961 aatcagcatc ttcttgagga aaatacaaga ctagaccata cgttaaaaac aaccacaaat
     4021 cgctattgca cattgttgga aagtgatgaa tggacgatca aacatagact aggacgacgt
     4081 aagaaaaaaa catcgaaaaa aatacaagaa aaagagcttt ctatatgtat tgatgagaaa
     4141 atatgggatg cagaaacaaa aatattaaaa atcatcggat ggggaatcgc taattcggat
     4201 cgccagccat tgtcctataa gttatcagct gaccaatcgc catttttcga agcaattcct
     4261 gtttcacgag atgaagtcaa tcaagcagaa cagctcgcaa aaggaacaga agcgggattt
     4321 gaattacgaa tcctttgcga gcaagaacgt tcattcttag tggaagcagt agctcaaaat
     4381 ggtcaatcat ggatcattga aatgtaaata atgattaact tataaagata aggagtaaac
     4441 gaatcgtgca aaaaaaagaa attgccgtat ttatcgatca aatcactcgt gaacgggcca
     4501 ctggcgattt gcttattgtg ggctgggcaa ttgatgaagt gaccaaagaa atccctacga
     4561 taaaggtaga aaaagaaaat gtgattgctg aagcaacaca tgtcgtaaga ttggatatca
     4621 atcatttata taatcttgat gttaagaccc aaagcggttt taagatacgt ttatccggga
     4681 aaatgagagg aaaagcgatc ttagattttc aaacggcaaa acatcaaaat ggcatagctg
     4741 tgaaactaaa tggtaaatac ccttatgatg acggcattga atcttcatgg gaaagaaaaa
     4801 aaagattact gaaaaaaggg ataaattatg caaggactca tggcgtaaaa aaaacaatcc
     4861 gccgggtcaa actggaattg aaaccaggat cgatcgacta tgcagagtgg atcagtcgtc
     4921 acgagaaacc tgatctgaaa gagcaaagga aagaagcaca agggtttgca taccgtccat
     4981 tgatttccat acttatgcct gtctataacg ttgaaatcaa atggctcgaa aaatgcattg
     5041 attcggtatt ggatcaaacg tatgatcact gggaactatg tatcagtgat gatgcttcta
     5101 ctgatccagc tatccgaaag tgtctggaat cctatcaagc aaaggatgat agaatcaaag
     5161 ttgttttccg acaagaaaat ggacatatca gtctggcaac taattctgcg ttggaaatgg
     5221 cagaaggaga atttatagcg ctcttagata atgatgatga attaccgcca tttgcattat
     5281 atgaagtggc aaaagtgcta aacatacatc cagaactgga tttgatctac agtgacgaag
     5341 ataaaatcga tgcagatggc aatcgctttg atccgcactt caaagcagac tggtctcccg
     5401 atacattgat gggaaataat tatatctctc acttaggcgt atatcgaaca agtattgtga
     5461 aaaagcttgg cggtttccgt aaaggctacg aaggctctca agattatgat ttggtccttc
     5521 gtgtgacaga acaaatccct gcagatcata tttaccatat tgaccgagtc ttgtatcatt
     5581 ggcggacgat cccaggatca accgcaagta atggtgaagc caaaagttat atctatgatt
     5641 ctggagtgaa agcattgaca gatgccctgt ctagaaggaa tatcaaagga agcgtacgtc
     5701 ctggcagaat ttctggattt tatgagattg cttatgacgt cttgcaagag gatttggtca
     5761 gcgtgatcat tccaacgaag aatggctatg aagatctaaa aacttgtgtg gattcgatta
     5821 tcgaaaaaac gtcttatcca aattatgaaa tcattattgc agataatgga agtacagatc
     5881 ctaaaatgca ggaattattt gcagaataca agcatcagct gaaagaccga tttatcgttg
     5941 aattaattga cattccattc aattattcac gtatcaacaa tctggcagca gaaaaagcaa
     6001 acggcaaata ctttttattc ttgaataacg atacagaagt gattgaacct gactggatga
     6061 cagcgatggt ttcttatgca cagtttgatc gaatcggctg tgtaggagca aaactgtttt
     6121 atccagatga tacaacgcag catgcaggag tattattagg aatcggtggt gttgcaggac
     6181 atgcattgaa taactatgat cgaacacatt gtggttattt cggccgattg gtcattgacg
     6241 tcaattactt agcagtcact gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg
     6301 tcaatggatt tgatgaaaca ttagaagtag cattcaatga tgttgacttg tgtttgaaag
     6361 tttatgaact gggtcgttac aatgtctatg cacatcaagc agaattgtat catttcgaat
     6421 ctaaatcaag aggttatgaa gatacaccag aaaaacaaag acgttttgcc ggggaaataa
     6481 aaaaaatgca agataagtgg ccttcttata ttgctcatga cccattttat aacgataatc
     6541 taactaaaga aggaatcggc gatttttcat tgagacctga ttgattaaaa gaactgtatt
     6601 ttcattttta ttgatgaaaa tacagttttt ttagtatatg aaaattgtaa cttgtgaccg
     6661 aagagatgat aggaagaaca cccattcagt aggaagaatg tgatacgatt tttgtccaac
     6721 tctctgttag gcagaaatat acatataaac atacaatagt caccttttct agtttctttt
     6781 aatttatttt acaatttggt tattcactag gtttttaaac tttgctatgg taagatgata
     6841 gaggcattat tttatggtta ggagtgtata tatgaataaa aatggggagt ggaatgcggc
     6901 acgtaggata ttcattatca tcatggatgt acttgtattg aatgtgtcga tctatcttag
     6961 ctttttgctg aagttccaag gagaagtccc agcaagaaac ttagagacct ttcagcattc
     7021 agcagttttt atttccatta tctttattgc gttgaatatt ttgttaggag cttatgtatt
     7081 ctatagccga gtgatcagtg atatcgtctt cattactgtt attggacagg tgctgatagc
     7141 attggggatc atggtagtta cttttgctgg acgttggttt gcatttccgc gtggcgtcat
     7201 cgtgctctcg tttatacttg gcacgatatt acttagcgtt tatcggatag ttgtttataa
     7261 actatactta agagtcagtc gtgataagaa agtccttatc gtaggtttgg agaaagatgt
     7321 cgcaccagct atccaaaact ttcaatctaa gaaaaacaac aaacataaag ttgaagcagt
     7381 agtgatcgac aagtattacg aaaacacaaa aaaattgatt gatgatatcg atattgtcta
     7441 cttggcttct aaaatcccag aagacgaaaa aatcaatatt tatcaattag taactaaaaa
     7501 agagaagaag ctatttttaa atacaacttt tgaaaattta acgatgatca atccgaacat
     7561 catgaatttc gaagatgaaa gtatcattga agcttccggt ttcaaaattc agccggaata
     7621 tgaattattc aaacgtcttt ttgatatcat cgtttcgctt attttactga ttcttgcttc
     7681 accttttatg ctaatcacag cgattttggt aaaaacaact tcacctggac caattattta
     7741 taaacaagta cgtattacga aaaatcaaaa ggaattttca atctataaat ttcgttccat
     7801 gagtgtaaca gctgaagcaa aatcaggacc ggtcttggca aagagcaatg atgcacgtgt
     7861 gacaccagta ggcaaattta tccgtgccgt acgttttgac gaattacctc aaatctttaa
     7921 tgtattgaaa ggcgatatgt cgatcgtcgg accacgtcca gaaagaccat ttttcgttga
     7981 tcagttcaat gaagaaaatc cttattatta tttaagacac aatgtacgtg cagggattac
     8041 cggatatgca caagtatacg gaaaatatgt atcagattat aatagtaaac tacgttttga
     8101 tttgttgtat atcaaaaagt attccttact attagatttg aagattttac ttcaaacaat
     8161 caaaatctta tttgataaga tgtcctcaca aggactagat gaagatgaaa atatccgttc
     8221 gttagcagaa atcgactttt cacaagagaa aatttatcgc tagatgggaa aaattttgag
     8281 caaagaagta ggagagaaca ttggaaaata aaaatataag tatgctgctt tctttatatg
     8341 ataaagaaaa accagagtat ttaaaggaag cattaactag tatttttgag caaacagtaa
     8401 tgccaaaaga aattgtatta gtttatgacg gccctataaa tgaaaaactt caagtagttg
     8461 tcacttattt ccaacaaaaa gatcctcact tgtttacagt tgtaaaatta gaaaaaaatc
     8521 aaggattggg gattgcttta gcaattggat tagaacatac gaaaaatcgc ttagtagcca
     8581 gaatggatac agatgatatc atggaacccc aacgattaga aaaacaatta gagatcttcc
     8641 aaaaagatcc agaaatagcg attgttggga gtaatattga ggaatttact ggcacgctta
     8701 gcaatgttat cggaagaagg attgtacctg aatataatga tgaaatccgt gtgttttcga
     8761 aaagaagaaa tccctttaat catatgacag tcatgtttga taaaaatgct attttagaag
     8821 tgggaaacta tcgcccatta attggatttg aagattatta tttgtgggta agactgttga
     8881 aggcgggata taaagggtat aatatccaag agattctagt ccatgctaga gcaggtgctg
     8941 acatgtatgc tagacgtgga ggaagtaaat atcttttacc aggaattaaa ggccgatatc
     9001 gcgtatggaa agatggtcta ggatcactaa aagattttct gatagttatt tgcggacatc
     9061 ttgtaattag tttattaccg aataaaatta ggggaaaagt atatgaatca aaacttagaa
     9121 aaacaaacta aactttctat aatcatacct ttttacaatg caaaaaatac tttaaaaaga
     9181 gctgtagaca gcgtcttaac gcaaggttat cccaatattg aaatcttgtt aattgatgat
     9241 ggatctacag atggtagtag tgacattggt gacgaaattg ctaatctaga gagtaaagta
     9301 gcatgttatc acttggaaaa cgctggtcca tcagttgcta ggaattgtgg gataaaagaa
     9361 gcaactggaa atttggtagg atttttagat agtgacgatt attttttacc tgattgtttt
     9421 aataatgttt tgaaagacta ccataggaat taccccgata tcattgcttt tggattaagt
     9481 aaaggaaaaa atatagaaaa tgcaaattta tttgttcccg aaaagaagat tggttgttca
     9541 agtgaagaag caattgagca aatgtttaga agtaaggcag tggattttta cgtatggaat
     9601 aaatttttta aaagggaatt attcaatcat atagtttttc cagaaaataa attatatgag
     9661 gatatggttc ctatgtatga agcttttaga gcggctagta aaattgatat tttgccattt
     9721 tcaggtattt tttattatca aaataatgat agcatcgttt atcagtcttt taattcaaaa
     9781 cagtatgata atattttgca aagaaaaata ttagttgatg aagtaaagaa agattatcct
     9841 cgacttatta atttggccaa tgctcgatta gtagacggat acctttcaac agggtttaaa
     9901 attactggca agaatcaaaa tcagaaagaa aagcaaaaat attataatca gtctaaaaga
     9961 gaaataaaaa gtatatattc aaaaataaga agtaacaatg agatatcaat tgctaaaaaa
    10021 ttagctttat ttttatactt aattaatcct aaaacttatt ttattttata taaaaaagtt
    10081 ctaaaaaaat aaagtttttt tcgatatgtt attttttatg tttattaact tcggaagact
    10141 aaaattgtta ctggtaatga tacatttgta aaaataaaaa agatgatttt taggaagtga
    10201 agagagtgca taaaattagt gttatagtac ccgcatataa cgtagaagac tatttagaaa
    10261 actgcttaga atctatttcc aaccagacac attcaaatat tgaaataata gtagtcaatg
    10321 atggttcccc ggatcgaagt ggggaaattg cagaagaata taaaaagaaa gatatgcgat
    10381 taaaagtgat acatcaagtg aatggtggtt taagtgcggc taggaattct ggtcttgatt
    10441 ctgcaacagg agattttgtg tgctttattg acagcgatga ttggatagaa ccagactatc
    10501 ttgagttact atattttggc acgcaaactt atggagcaga tatctcggtg ctaaaaataa
    10561 aaaagatttc aagtttagaa caactaaaag aacatacaat tgctgagaga aaatgggaaa
    10621 tatttgatag agaacaagca atgaaaaact tatttataaa taataaaatt ggctattctg
    10681 caaataataa attattcaat agaaatttat ttgataatat aagattccca ctgggtaaat
    10741 taatggaaga taaggctact acttatttgc tgatcgatca ttccgaaaaa gttgctgtca
    10801 attcatctgc taaatatcac tattaccaaa gccctaaaag tatactgaga ggaagcttca
    10861 atcctagaat ttttgattct tttgaagtcc atgaagatat actaatattc atagataagt
    10921 tttatccgga attatcaatt aatgttagaa ctcgctatgt ctatgaagca ataaggatga
    10981 tgatgacaat gattaaaaat aactatacca ataaagaaga ttatttacgt tctataaata
    11041 ttttacaacg ctttgaaaaa gaagtgaaaa ttgatcccga cttttcacca aaattaaaaa
    11101 aattagtaaa gtttttatta ataaataaaa acttgcctta ctatatgtca agtagtaaga
    11161 tagcaagtaa attcttgatt aagatagaat ctgtgaggta agagatgaag aatataaaaa
    11221 aatttttttc aaggaatatt ttcccggttt ttattaaaat attacctatt caaaaaaatt
    11281 taattgtatt ttcaagtttt ggcgggcgta actattctgg aaatccaaga gttatttcag
    11341 agaaaattca tgaaaaaaaa ttgaattaca gaattatttg gttaatcaaa aaagaagcga
    11401 ttataactga tttaccagat tatattaaaa gaattgactc cgattctttt ttagctgcct
    11461 tttatttata tacagctaag tattggatag acgatagtcg gaaaacgatt aagtatgcaa
    11521 aaaggaaaaa acaactttat tttcaaacat ggcacggaac gcctttaaaa aaaattgaat
    11581 ttgatgcaaa agataagtta cctaaacgtt acctaagcta tgcgaaaaaa gatagtgagt
    11641 caattacata tttattatct ggtaatcgct atagttctaa gaagtatgtt tcagcattca
    11701 atatcgttcc ttctaaaata ttagaagtag gaactcctag aaacgatgaa ttagcctcta
    11761 gtaaagagca agtgtttgac ttgaaaaaga aacaaatcaa tgtcttattt gctccaactt
    11821 ttcgaaataa tattgaggac aacggaatta cgcaactaga atggttaggt gtggataact
    11881 tacgtgaatt ttttaaaaag caacaacaag aattaaattt tatgactaaa tttcatccta
    11941 atgttcatag taaattagca actgattcta aaagtataga atatatgaaa aaacatcaaa
    12001 ttactttaat taatgatggg attcctcttg aaatgctttt tgaagagata gatgttttaa
    12061 tcacagatta ttcatctatt tttttcgatt ttgcgctaac aaaaaaacca attattcttt
    12121 ttaactatga tgaaaaagaa tatagcttag aaagaggttt ttatatacag ttaagtgagt
    12181 taccaattat aagcgtacaa gatagttatg gccttgttga tgtttttaaa aaccaacaag
    12241 aaaagctttt gaaaagttct caagagttac ttgattatat tggaaattat gaaacaggag
    12301 caagtacaga aaaggttctt gagttgatag agagggatat aaagtgaaaa aaacagtaaa
    12361 agatatattt tataatgcga tatatcaagt ctttttgatt gttttaccct tacttactat
    12421 acctatactt agtcgtagaa ttggttcaac tggtttaggg atttatgggt atgttttctc
    12481 aatatcacag tttttaatga ctgtcattgc tgtgggaatg aatccttttc gtattcgaaa
    12541 tatcgctaaa tcaaggaaag ataaaaaagc actatcttta caattctgga atatttactt
    12601 tattcaattt ttaataggct tgagtgtttc ctttctctat atagcctata tttatttttt
    12661 tgatgtaaag tataaaaatt tatacttaat tcaattgata tttattgtag ggctaacatt
    12721 agatatttct tggttttttc aaggaattga agaattttcg aaagtggtta ttagaaatac
    12781 agttatcaaa ttatcatcgg tattgttaat aatcattttt gtttcaacta aagatgattt
    12841 gtggatttac gtgttcatta cttcaattac taacttttta ggaagtttag ttttctggtt
    12901 aagtatccac ggaaaagtag gtaaaccaat ctttaactct aatgttttta aacagttgtg
    12961 gaaaccagct tttattattt tgactcctca gttgtttatg caagtgtaca caactttaga
    13021 caaaacgata gttgggtatt ttgtggatcc aacggaatta tcttattatg atcagtctca
    13081 aaaaattgct cgtattatat tggcagtctt aacatcaatt actattgtta tgttgcctaa
    13141 aatatctaaa gcacaggcag aggggaaatc agaaaatatt ttagtttaca caaaaaaatc
    13201 ttttgattac acgattatca ttgcattaat tctttttagt gtggtgttta tcaatacaaa
    13261 agaatttgtt ccatggtttt ttggggagaa tttcaaaccg atgacaataa acatgcttat
    13321 ttcttgtcta attatagttg ttagtccaat tggtggagtt tttgctaacc aatttgcaat
    13381 tgctatagaa aaagataagg agtatgctta tccaatgatc attggtgcga ttgtaagtat
    13441 tattggaaat gtagcttttg tgcctactta tggcgctgta gcggctacga tcgtactcgt
    13501 ggtagtagaa ttattagtct ttttctttag agtatttttt gtacgaaaag aaataaaaat
    13561 atcctatttc tttgggaaga acgttatctt attttttata gtcaatctta ctttgactac
    13621 ttgggcctat tactttcttc cacaattatt ctcttcttca tttttagata tggcaattaa
    13681 atcaataatg attttcatta tttatcttcc gtttgtttat tttctttttc cagatatacg
    13741 aaatgaaata agaaagatta ctcgaaaata aaataatgtt gtaaaaaagt agttctttgt
    13801 caaatcgggc tgataaggtc atttatgaac aacaaagtta gtaagagtta ctagctttta
    13861 atgcatagct gattaacctg ttgattagaa aaatccacaa gcgcatattg gtaaaactgc
    13921 gaaacccata agcaaatcgt ttcaatgttt tgatatgtgt gcgaagattc tctaattttg
    13981 ttattagaat actttttcga aaatgctagt taaaagcctt tttcagatat gaaaaaggct
    14041 ttttgagata gtgaagtttc gagggaactt ctttggtcgt ttctcgaagt agctagctgt
    14101 ttcttgtgat aattgctaag cagcttttga taaactgctt agcaatttta agttcataag
    14161 agattgaggg tagacgatca actacttcac tttctgttaa atagcgccat tgaaaattct
    14221 gccatttctt gaatgttgtg taatccaact cgtcatctga ttttagaagt aatttataca
    14281 aaggtttcaa ttgtcgatag tacttagctt gtgaagtgtc tttttgattc aactgcttca
    14341 tcacacgttt tcgaacacga tcgaaaaact tgttcaagtg ttgtccaatg tgaaagcaat
    14401 caatgatgat ttgggcgttt ggaaaggact ctttgactaa tgaaaaataa ggggcattca
    14461 tatttgtgac aatgtatcga acccccatat gagcttttct tgtaaaacgc atgaaataag
    14521 caatcaaatc tttttttctc cggctggaca aaatgtcaaa gagcttccct gtctctctat
    14581 tcatacaaga aaaggagaac tttccatggg aaattgaaag agagcgaaac tcgtcgaata
    14641 gtaaacaatg aggtaaatag tctagcatct gtcgcagcat tgccgataga gcagataatg
    14701 tccgttgaaa tgtcgttact gaaagatcac aacgtttcgt aagtgtcttt aacgcttgac
    14761 gctcggtcaa ctcttctaat atcgtgtaat gaacttgttt cgaaataaaa catgaaggaa
    14821 cgacaagata cgtatgagca gtaaaatatt ttccgcatga ttggtagtgg taatgttgtt
    14881 ttctaagacg caaataggtt tatttattcc cactgcgatt taagataatc attgaaatct
    14941 tcttgccgtt tttcacgagc tgcttcgcag tttctttaca aaatagacaa tgtctaaaag
    15001 tgcgaactaa tgtaccttcg attacgatgg ctttcttgtc cttataaaaa gttttttgaa
    15061 aagaattaat ttcaatattt ttatctttaa tagtgaagtg ctctttgata gaacgagaaa
    15121 tggatataca aaatcttcct ttcagattga tgttttagac gactctattt tatcgaaatt
    15181 tttgtatgtc tgtttttttt acaagaaaat ctaggatcga cagaaaattc tatcagtcct
    15241 agaaattata gagccatttt tttacgtctt atccagatat attgttcttt cattgtttaa
    15301 aaaatattat ttatcactat ttaaagaatt aagttcgttc tttatttgag ttgtagagat
    15361 ctcaggtgtt cttggtaaat aaattacttg tgcatctgtt agttgttcaa gttcatcaaa
    15421 ttgtccagcc caatcatcgc ccatcacgaa ataatctgca tggtattctt ttatatcttt
    15481 tactttttgt tcccaatttt cttcaggaat aactaaatca acgtagcgaa tagcttcaag
    15541 taactgtttt cttttttcat aactaaaata acattttttt tgtttcgcat tccaattgaa
    15601 ttcatctgta gaaagggcaa caataagata atctccatat tgttttgctc gtttcagtaa
    15661 attgatatgc ccgtagtgaa gcaaatcaaa ggtaccgtaa gtaattactc ttttcatctt
    15721 tcacctctcg aaattcatta atatacattg ttagtttagc atttttttgt tagaattgaa
    15781 agaaccagtt tatcctaaaa aggataagaa atgtttatga gaaataggtg gatggaggaa
    15841 aaatgttgaa tgaaaaattt ttaaaatatg tgaaaggttg gtattttatt tattttggtt
    15901 ttattctaat gttacgagca ataggcccgt atttactatt acctactaga tatgatactt
    15961 tacttttttg gcctgctgga atagcgggag ttgtcttgat cattatagat gcaattattg
    16021 catttagaga aaaaaatctt aaaaaatatg attggctatt aattgctttt attggcgcga
    16081 tgctagtaag cagtttaatg aatttaaaat atggatatag cgaaaatatc aagttaatca
    16141 tgtggcaatt aatctttttc tttatgattt atcagtttgg caaagactac agtagtaact
    16201 tttttttcaa agtatttact tggatacttt cattggtatg gtttttagct aatttagtat
    16261 cattatactg gtttgtcatt caatatggca taaaaattcc aataaaatat aagtattacc
    16321 cagttcgttt agggtggtta ggcaaccgtt tatttggtgt atatacagat ccaaattttg
    16381 gtgcggttat atcattaatt gtcatcatta ttgctatata ttatttggtc acttacaagc
    16441 atttaaatct tctatttaaa atattttttg caattaatat ttttattcaa ttctcgttta
    16501 ttgctttatc gggctcacgc accacattag tttgtttatt aatagcaact tttgttttta
    16561 gtttctttac aagttatagt agtcgttttc ttaaaactaa tactttaaaa agatggctaa
    16621 ctgcattagt agtaggattg ctaagtataa ttgcgatgta ttatgtaaca gatataactg
    16681 ctcaagcatg ggcacatgtt ccgcatatta cagaaataaa aaaagatata tctgattcat
    16741 caggaacaac tagcagcaat aaagttgatt taaaacgccc tgacactgat ggcggggatg
    16801 tttctaattt aaggtttgga ctttggaaaa gtgcaattga aatattcaag agtaagccga
    16861 tatccggcgg ttctccagga tactatattg aatacgcaca tgataaacta ccatatacat
    16921 taatgggaaa agatacttta actgctcata attttatttt cttagttatg gctgctacag
    16981 gaggattagg tctattgaca ttgttaacct tcttatttat ccaatgcttt aaaactttgc
    17041 gttattgttt cacacaacca aatctagtaa aagataagca cttttttgct attttaatga
    17101 ttcttacaat agcagtttcg gcattattta tcacagaact cattttggtt agtacgatag
    17161 gttcatttat tttttgggga tatctgggtt ctatccaaaa aactatatct aaagaagaag
    17221 gagtaactaa ttgaaactat ttttaggtat ataaagtaga aaagaaaact agttatccca
    17281 acttcaacaa attttatagt ttttacaata taaaaatggt aaactaactt tattaagtat
    17341 gagaatgcat aattggagtg aatttgatgg atactatatt gattactggt ggagctggtt
    17401 ttatcggttc aacgctagcg aattatttgg gaaaagaaaa caaaatcgta gtagtcgacg
    17461 atttatcaat gggaaaaaaa gaaaacttgg atatggagaa acaagtgact ttcattgagg
    17521 gggatgtatc agatcctcaa ttgatggaaa gaatcatgaa acagtatcag tttgcctata
    17581 tttttcattt agctgctgta gctagtgtgg cagattctgt agaacgtcct ctagaaactc
    17641 atcgtgtaaa ttttgacagt gcgcttttat tgcttgaatt ggtacgaaaa tatcaatctg
    17701 gtttaaaacg attggtattt agttcatcag ctgcagttta tggcgatgaa ccaacgttgc
    17761 caaaaaaaga agaatcggtc atccgtcctt taacaccata tgccatcgat aagtttgcat
    17821 cagaacaata tgttttggat tactgtcatt tatatgatgt tccgactagt gctgtccgat
    17881 tttttaacgt ttatggtcca aatcaaaatc ctaactctcc atatagcgga gttatttcga
    17941 ttctagtgga ccgatacaaa aaacaattgg caggagaaaa aacagaattt actttatttg
    18001 gtgatggtag ccaatctaga gacttcgttt atatagaaga tgttatacaa gcacttttac
    18061 ttgttgctaa ggaagaaaaa gctttaggac agcaattcaa tgtcggtaca ggaaaaagca
    18121 ctacgctact tgaattgatt cattcaattg atcaaatctt aggaacagag ctagcgctta
    18181 aatatgaagc ggagcgttca ggggatattc gtgattcttt agcggatatt tcaaagatcc
    18241 gttctttagg ttatcaaccc aaatttgata tattgaacgg aatggaacgt tatttaaaga
    18301 cagaaatcga ttgaaacaaa aaaccagcat cctgctggtt tttttgtctc gacttagctt
    18361 tttaaattaa caccttccat cagttcgtaa atctgatttc ttaaatcagt ggccggcaat
    18421 ttgatgtttt tatcagattg gatattactt tccatgacga ttgcatcttt tgtgtctttt
    18481 ttcatcgccg ctactgcatg gaatcgattg atatttccct gagcaaggat ataatcattt
    18541 ccactgtaaa caccgccgct gtaaggatgt ttatcaccat tattttgcca caatgagaaa
    18601 agtagttctt ttggtataat atcgccagac agaacactgg taaagaattt gttcaagtca
    18661 taaacagaca tacttatatt tcctgtgcct aattcatttc ggatgtctgt ttcaggctcg
    18721 accaatgctt ttgaataatc gtcagttgca gacatttggt aagatacagc atgctgaggg
    18781 gaatttttta cattttcata aaaatctgta tgttctagat ggagtggccg aatgatttct
    18841 ttttttaaaa gagtttcata aggctgattc attagtttga tcaatacccc tgtaagcaat
    18901 gtataattta atggttcata atgatacgta tcataaccag taaatttcaa atgatgaagg
    18961 gcaaactgaa tgatctcact atctgacata ggtttttttg gagaagcggt tcgatgaaga
    19021 ccagagcgca tgtaaagcat atttttgatt gagatttgct gacttccagg aatctgagga
    19081 taaaattcag atagctttgt gtcaaaagtt atcttgccag attgaatttg ttttaggata
    19141 agtaaagcag tcatcgcttt ttggatcgaa ccgatttgat agtaaagaga aggagaattc
    19201 gattctttta tgcgtagatt tgcgtaccca tatccttttt ctaatatcac tcgattattt
    19261 ttgcgtacat aaatcgaacc gatataatct gatttttgca gtaattgatc aattttgctg
    19321 gcaagcatct gctcattaga tgttaaattg gaagcaagtt gtgcgggttc cgttttataa
    19381 atttgtggtt gaggttgttt tgttaattgc catcctgtaa tgatgcctgt tgaaaagaag
    19441 ctgacagcca gcaacaaaac tgcgagaaaa gaaagagaga tctgctgttt tttttgatgt
    19501 tttccttttg ccatattaaa tcctttctat ccatttttaa ctatttttca ttgtaatcct
    19561 caaaaagagc atttgccact tacaattttg acataatatt tggtaaaata attcacggca
    19621 gtagaaattt catgtataat aaacagagtt acgaagatga tagtaaggag gagttgtttt
    19681 gtcaggaaac aaacaacacc caagcaaaga caaaaaagaa gccagtaagg cagctacagc
    19741 agatataatc gacaagcgga gaaatctgcg taaaaaagaa gataagatcg tgaggaagat
    19801 cattttagtg atcgctctta cattattgat tattggcgga tttctcgggt ttaccgtata
    19861 tcgttatgta gatagcgggt tgaagccttt agataaatcg gacgaccaat tagtccaagt
    19921 ggaaatccca aacggctctt ctaataagca gattggcgaa attttggaga aagacaatat
    19981 tattaaaagt gggattgttt tcaattatta tacaaaattt aaaaacttaa ctggctttca
    20041 agctggttat tatcagttag cgcctaatat gactcttgat gagattggca agcagttgca
    20101 agaaggcggc acatctgagc caacaaaagt tgcagatggg aaaattgcca ttccagaagg
    20161 ctacgacatc gatcagattg ctgagcgggt agcgaaggta acaggtaaag acaagaaaga
    20221 atttttagat ttagtaaatg atgagacttt ctttaacgag ttgtatcaaa aattcccaga
    20281 attgttggaa agtgcctcta aagctgagaa cacaaaatac cgtttggaag gctacttatt
    20341 cccagctact tatgattact acacgagtac ttcgcttaag gatcttgtga ttgagatggt
    20401 gaacaagaca aatacggtca tgcaaaacta ttattcagct atcaaacaga aaaatttgac
    20461 tgtacaagaa gttttaactt tggcttctct agttgaaaaa gaaggcgtaa aagaaaatga
    20521 tcgtaaaaat attgcacaag tcttctttaa ccgaatcaag gcaaatatgc cacttcagtc
    20581 agatatctct gttttatatg cattaggaga acataaagaa ttggtgacgt atgaagatac
    20641 agctgtagat tcaccataca atttgtatac caataccggc tatgggcctg gtccttttga
    20701 caatccaagc gaaaaggcaa tcaaggcagt attagaacca gcagaaaatg actattatta
    20761 tttcgtagca gatacgagca ctggaaatgt ttatttcgct aaaacgtatg aagaacatat
    20821 ggaactagtg caaaaatatg taaataattc ttagaaaaac aggggctagt cattgatcat
    20881 ttttagaaaa acatttacat tttaagaaaa gcgtggtaat cttttttgga ctatattcct
    20941 caaaagatta ccttttgcgg tattttatcg aaaaaaggag acgttacaca tggtagagaa
    21001 agtttatcca atgactctag aaggtaaaga aaaattagaa aaagaattag aagaactaaa
    21061 aacagttaaa cgcggagaaa tcattgaacg aatcaaaatc gctcgcggtt tcggagatct
    21121 ttccgaaaac tccgaatacg aatcagctaa agatgaacaa gcatttgttg aaggaagaat
    21181 cacaacaatt gaaaatatga tccgttttgc acaaatcatc gataatgacg gcgtagatgc
    21241 agatgaggta tcgatcggta aaacagttac gttcatcgag ttgcctgatg gagatgaaga
    21301 agagtacacg attgtcggaa gtgctgaagc agacccgttt tctggaaaga tttccaatga
    21361 ttctcctatc gcgcaagcat tgattggcaa acgtaaaaat gatgaagtaa ccattgctac
    21421 accaggtggc gatatgttag ttaaaatcgt caaagtagaa caagcgtagt ctgactgaaa
    21481 gatctagaga gttcatctta ctgatgacgc actctagatc ttttctcttt gagtattgca
    21541 ggtttcactg aacgatctta catctaaagt cctatagcca acagtcaaaa aaataggtat
    21601 gatgaaatga gaatgtgtca ggagggagaa aaagcgatga atagttcttg gcgttttttt
    21661 gtggtagtcg aagcactgct gctgttattt gctgtttggc aaatcgtaaa caatactgga
    21721 cttttgcttt tagtaatatt cggtattttc aatatatatc tagctatgcg caaatatcct
    21781 cgtactaaat ttcaaaattt tcaattgatc ttagggagct tggtcatttt ttttagctta
    21841 gtcaacagtc ctgctttatg gatgatggtt gtattggccg ttttatttat cgggctgaaa
    21901 ggtgtagaga tttcaggaat agatttgaca aaaaatgctt tttggcgaaa aaaacagatc
    21961 atgatggtcc aaacagagca attaaaaaca cataataatg agcgtaaaag acagcaatta
    22021 tttggtaacc aacgtattgg aaacgacgtc tatgaatggg atgatatcaa cattgccatc
    22081 atttctggtg atactatcat cgatttgggt aatacacttt tgccaaaaga tgataatatt
    22141 gtgattgtaa gaaaagggat aggcagaaca cggatcttag tacccctggg agtggctatt
    22201 cgtttagagc atgcgacatt agtaggaaat gtcttgtttg aagaagagca gttttcctta
    22261 aaaaacgaac agatcaaaat ctgcagcagt gattatgatg aaaatcctcg cagattgaag
    22321 atcattacca ataccttggt tggagatgtc gaggtgatac gaatatgatg ggaagaatat
    22381 ccaaagcaat gctagctgtt tattcgggga ttgcggcttt ccttattatt ctattttcac
    22441 ttttcactta tttttatgcc agtaaccaaa gtcattggtg gggagaattg ctgcgtgcgc
    22501 gtttattata cgttccgctt atcttccatt tgatggcaat ttcattaggt gtaggattga
    22561 tcgtatttct attgttatca cttattcaaa aggcaaaata cgggaaaatc gaagaaaagc
    22621 ttcgtgcact ttcttctggc aattatgaat ccaaactttt gcatcagccg atcccaagcg
    22681 cgtcggatga tctgtacatc aaagatattg atatggaaat tgctaaaata aaagaaaaaa
    22741 tgatcgaagt atccagtgaa ttacagatta tgactagccg tcctcaatat gtagatggac
    22801 aaaccaaaga agagatttta gaattagaaa ggcatcgatt agctcgtgag cttcatgatt
    22861 cagtttcaca gcaattattt gcagcgatga tgatgatgtc ggcattgacg gagcaagcag
    22921 aaaaaagtga gacaccagaa atgttccgta agcagttgaa aatggtggca gagatcatca
    22981 acgcttccca gtctgagatg cgtgcgctgc ttcttcatct gcgtccagtc aatttagaag
    23041 aaaaaagttt aaaacaaggc attgagcagt tattgaagga attgcagaat aaaatccaga
    23101 tttcgctaaa atgggatgta gaagatgtaa aattaactag ttccattgag gatcacttgt
    23161 tccgaatcgt tcaagaattg ttatcaaaca cattaagaca tgctaaggct aatgaattag
    23221 aagtctattt gcataaaata gataacaatc ttttattacg tatcattgac gacgggacag
    23281 gatttaatat gaatgaaacc aaaacgggaa gttatggatt gaacaatatc aaagaacgag
    23341 tagctggtat tggtggaaca gtaaaaatca ttagttttaa agggcaaggt acgagcgtgg
    23401 agatcaaggt ccctttgatg aaggaggcat agataaagat gataaaagtt ttattagtag
    23461 atgaccatga aatggtacgc ttaggcgtct cttcttattt atctatccaa gaagacattg
    23521 aagtgattgg agaagctgaa aatggacgtc aaggctatga aaaggcgatg gcacttcgac
    23581 cagatgtcat tttaatggat ctagtcatgg aagaaatgga tggcatcgaa tcaacaaaag
    23641 caatcttaaa agattgtcca aaagccaaaa tcattatcgt cacaagtttt attgatgatg
    23701 aaaaagttta tcctgcaatt gaagcaggag cagcaggata tttgttgaag acgtctaccg
    23761 cacatgaaat agctgatgct ataagggcta cgcaacgcgg cgagcgagta ttggaacctg
    23821 aagtgacaac aaaaatgatg gaaaaaatga gtcggcgaaa tgatccagta ttgcatgaag
    23881 aattaacgaa tcgagaaaat gaaattttga tgctgatttc tgaagggaaa agtaatcagg
    23941 aaatcgcgga tgaattgttt atcacactga aaacagttaa aacgcatgtg tcgaatatat
    24001 tagctaagtt agaggtagaa gaccgaactc aagcagctat ctatgcgttt aaacatggtt
    24061 tggtgaaata aaaacgatga taaagaggat aatcaaaagc tgagaaaata actattattt
    24121 ttcttggctt ttttacaata atgatgcttg cttttgtttt tggaaaggct ttgttatact
    24181 gagaaaagaa gatactaaag gaggaaaatg gtgaaacaaa attttgcaat tgtaggcctt
    24241 ggacgatttg gcggaagcat ctgccgtaca ctgatcgaag ctggacaaga agtactggct
    24301 attgacagta atgaagatcg agtcaatgaa tatatgaata tagccactca tgctgttgtg
    24361 gcaaatgcac aggatgagat gacacttcgt tcattaggta taagaaattt cgatcatgta
    24421 attgtcgcta ttggagaaga tatccaagcc agtatcttag ttactctgat ggtaaaggaa
    24481 atgggcgtac cgaatatttt agcaaaagcc caaaatgagt accatgcccg tgtactagag
    24541 aagatcggag cagatcgtgt cgtacaccct gagcgagaca tggggatacg gattgcacat
    24601 aacttagtat caaagaatat tctagactac ttagagctgt cagataaata ttctttagca
    24661 gaaatccgag tctccaatcc taaatttttc aacaaaacat tagctgaatt aaatttcagg
    24721 caacgttttg acttaacagt agtcgccata cgaagagtag atcaaacggt tatcgcttct
    24781 cctgcagcag atgaatatgt tcgcgaaaat gataatctac tggtgattgg agagacaggc
    24841 gatgtggata ttctagacga taagatgaat caatagaaag aaaagagaaa gtaggttgtt
    24901 gggatggaat taacggtaac gccaaaagca caaaagtggt ttgactcaga aattgatctg
    24961 ccagaaactt acggaatacg tttttttgga aaagtctacg gcaaaacaga ggttcatgat
    25021 ggtttttcga ttggaatgtc agttgaacag ccagaacatc ctgtaaagaa agtggaaatg
    25081 gatgggacac ttttttttat cgaggaagca gatgaatggt tctttaaagg atatgattta
    25141 cttgttgatt atgatcccaa cctggaggag ccaacctatc actttaaaaa acaataaaaa
    25201 aagagagggt gacacaagtt t
//
LOCUS       DACDYM010000036        24747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDYM010000036 DACDYM010000000
VERSION     DACDYM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.036"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(57..173)
                     /locus_tag="IWM59_001487"
     CDS             complement(57..173)
                     /locus_tag="IWM59_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9979526.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(418..1332)
                     /locus_tag="IWM59_001488"
     CDS             complement(418..1332)
                     /locus_tag="IWM59_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9979527.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(1347..2348)
                     /locus_tag="IWM59_001489"
     CDS             complement(1347..2348)
                     /locus_tag="IWM59_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9979528.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            complement(2345..4528)
                     /locus_tag="IWM59_001490"
     CDS             complement(2345..4528)
                     /locus_tag="IWM59_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9979529.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            complement(4531..6978)
                     /locus_tag="IWM59_001491"
     CDS             complement(4531..6978)
                     /locus_tag="IWM59_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9979530.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(6962..7354)
                     /locus_tag="IWM59_001492"
     CDS             complement(6962..7354)
                     /locus_tag="IWM59_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9979531.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            complement(7441..7938)
                     /locus_tag="IWM59_001493"
     CDS             complement(7441..7938)
                     /locus_tag="IWM59_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9979532.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(8055..8279)
                     /locus_tag="IWM59_001494"
     CDS             complement(8055..8279)
                     /locus_tag="IWM59_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979533.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(8383..10293)
                     /gene="ltrA"
                     /locus_tag="IWM59_001495"
     CDS             complement(8383..10293)
                     /gene="ltrA"
                     /locus_tag="IWM59_001495"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9979534.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11077..12282)
                     /locus_tag="IWM59_001496"
     CDS             complement(11077..12282)
                     /locus_tag="IWM59_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9979535.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(12305..12458)
                     /locus_tag="IWM59_001497"
                     /pseudo
     CDS             complement(12305..12458)
                     /locus_tag="IWM59_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(12461..13846)
                     /locus_tag="IWM59_001498"
     CDS             complement(12461..13846)
                     /locus_tag="IWM59_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9979536.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(13875..14261)
                     /locus_tag="IWM59_001499"
     CDS             complement(13875..14261)
                     /locus_tag="IWM59_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9979537.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(14277..14591)
                     /locus_tag="IWM59_001500"
     CDS             complement(14277..14591)
                     /locus_tag="IWM59_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9979538.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(14944..15423)
                     /gene="rlmH"
                     /locus_tag="IWM59_001501"
     CDS             complement(14944..15423)
                     /gene="rlmH"
                     /locus_tag="IWM59_001501"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9979539.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15851..17140
                     /locus_tag="IWM59_001502"
     CDS             15851..17140
                     /locus_tag="IWM59_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9979540.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(17179..17784)
                     /locus_tag="IWM59_001503"
     CDS             complement(17179..17784)
                     /locus_tag="IWM59_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9979541.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(17980..18444)
                     /locus_tag="IWM59_001504"
     CDS             complement(17980..18444)
                     /locus_tag="IWM59_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9979542.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(18466..18786)
                     /locus_tag="IWM59_001505"
     CDS             complement(18466..18786)
                     /locus_tag="IWM59_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9979543.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(18929..20008)
                     /gene="pepA"
                     /locus_tag="IWM59_001506"
     CDS             complement(18929..20008)
                     /gene="pepA"
                     /locus_tag="IWM59_001506"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9979544.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20208..20558
                     /locus_tag="IWM59_001507"
     CDS             20208..20558
                     /locus_tag="IWM59_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9979545.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            20743..21696
                     /locus_tag="IWM59_001508"
     CDS             20743..21696
                     /locus_tag="IWM59_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9979546.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            21709..23496
                     /locus_tag="IWM59_001509"
     CDS             21709..23496
                     /locus_tag="IWM59_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979547.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            23483..24685
                     /locus_tag="IWM59_001510"
     CDS             23483..24685
                     /locus_tag="IWM59_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979548.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         6992 a         5007 c         4518 g         8230 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggtg caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
      361 tttttatcaa agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta
      421 ttctataatc ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg
      481 cgttgctttg gtctgctggt cgatatactc cactgtcagc gataccgtga cttgattatc
      541 cttacgattg taaataggat ttaccagttc ttgaaagatg tactcttttc cgattggttt
      601 taatatcccg tcattcacat agtaggaaag ttcactggct gtcgctgtag gatagagctt
      661 gaagaacgtc gttaaaaact cattgatttc attggttgta atggaatcaa ccgtaccctc
      721 actttcaata gcttttggtt tatagtctga tttctttggt atgctagtaa tggtcggatt
      781 cttaatgagg accatatttc cagtactgtc tacatagaca ctcactatat aagcagagtg
      841 gacggtcttt gtattttccc cctctgtaat gagttggtct acactgtagg ttacatcaaa
      901 ttgattgtcg ccatctgctt ctaccgtcca tatctgaaat ccttttacag aagacgatac
      961 aggaatatct ttacgaactg tatcgacatt cagagcttga agttcctctg tcagatatgc
     1021 tttcaggttt tctatgcgat tatcaatgga cttgtcgttt agttcccatg aatagtagac
     1081 tttcgcaaag ttctctacaa aattttccac atgatgagta tcgacatatt ccttttctat
     1141 gatggtggtt tcatgaatgg tatgggtatc aatggctgta aagtgcttga atatcgcaaa
     1201 gctgaaacta agtcctaaaa gtacccatag gacaatcaca acctttttac gaggattgac
     1261 cttatagaca cgaggtttct tttcctttgg tgtctgtttt tctgtattct gattttttcc
     1321 gaatttcatc attgaatcgt cctttcttat tgtttgattc gtcctgctcc cactaaatgc
     1381 tgttgccagt aagagcttgt taagtcggca taaccgatag ggtcgcctgc atgaaacata
     1441 cggttatcgc ctaaataaat cccgatatga gtaatataag agccagcgtt ataggtagaa
     1501 tgaaagaaaa ccaaatcgcc ggcttttgct tccaataatg ggatatgccg ggttacatca
     1561 tattgctgtt gtgctgttcg tggtaagttg attccagctt ttccatacgt ccattgtgtc
     1621 agtccgctac aatcaaaaga ggtagtcggg gaagctccgc catagacata cttccagcct
     1681 tcaaatttca gagcttcgtc catgatggct tgtactgtat catcatcaaa ctctgttgtg
     1741 acaagatact gcgttaccag ttgcacataa aacatattcc cgtaattgta tcgccagccc
     1801 ccattgatag gtatggctat gggattgggg taagacactt tttcgtcacc cgaatactct
     1861 tttgagaaac tttgagccag ttcaaaggta tatttattcc cacgattagc cacatatcct
     1921 aagaaaccac caccataatt gtaggactgg ataaccgatt ctaaatctac accaagcctt
     1981 tcgctactgg ctaataactc actgaaatac ttcacacctt gcttaatgga ttcttctgta
     2041 ctcaatgaat taggtggaag accgagggat tccgaggact gcataacatc ttctgctgta
     2101 ccacccgatt ccacctgtat aatcgcaaga agtatattga cgtattcttc aattccatat
     2161 tctttggcaa ctttttctac cataggctta tgagccagca cttctgcgga aacatccaca
     2221 ccgccatagt gaatgttgga aaaaccgcca tcttgttcat ctgaaaataa aatggcgaca
     2281 aacagaagca gtgagaagac catcaagaat aatcccgaac caccaatcac taaagttttc
     2341 agcttcatgg tttcttactg cctttcttaa tggtggcggt tttgattggt ggtctacttt
     2401 ttgtattttg tagtggtact ctttgaacag tagactgacg ttcttttgtg attgaacgtt
     2461 gtgaagctct atctgttggt gcagttgaag ttactggctt ttgaaccgtt ctttcttgaa
     2521 ctgtattact ttgacgttcc gtttttaaac ttgaagaatc ggacttaact gttggacgct
     2581 cttgtttggc ttgttgagat tccctatagg aagcctgtac agttgtttgc cttgaagtct
     2641 gtccatcatg agattgttct tgcttagtgg ctggtctttc atgaacggaa gaagctggct
     2701 gttttttctg tttgacctgt tccatttcag agcgacgctc cgcaatggtt ttccgtcttt
     2761 gttcctgctg tttcttgcgt ccactggctt tatccgcttt ggtttgagaa atacttctgg
     2821 ttaaatcatg gacattatcc gtcactttag attttccttg atatactgca tatcttgcat
     2881 tggtcggcaa atccttaacc tgttctttca aattaccagc agtatctacc attctgtctt
     2941 tggtatcagc tactgtaccg atggtttgac cgatacgctt tcctagtgtt gatttttcct
     3001 gcccgtttgg tcgagagtga tctgcttgtg tactcgcaga acttcccgaa cccgactgtc
     3061 cttttttacc cgtagcactg gcaatggcag acccagcccc taaagcagtc acggagcgtc
     3121 caagtttccg ctgtagacgg tgcatgtgag cgtgcataag catacgaggt tttctcatca
     3181 tacgacttcc cacactttga gaatcgttac tctgtagaga aaacatactc attaaatcgc
     3241 ccagcttaaa gtagattcct gcaaaggtca caatctgtag aaaagcaatc aaaaagaacg
     3301 gataaccagc cgataaggta tagagcatgg ttgaaatact aaatgctgtc gtaataatca
     3361 atgtgattcc agctcgtgtc aaaatggtat taaagagctt tgttatggct cgttttgaca
     3421 taccatcaaa tgatggaatc atgcttaaaa taaagctcac aggcagaaac atagcataga
     3481 tgataaaaag tacctgcgag aaaatcatga ttcctgttaa taggaataca aatatggaaa
     3541 tcccaatatt gaagacaaat aggaagaaga ctgtacctaa acggttaatc gtctttgtaa
     3601 tcgtcatatt ggtattgctt ctatcttcaa tttcttccgc aacaattttt tctctgtctt
     3661 ccccattgtt ggaatctgga ctggttgaga gcaggctttc cacacggtca ataccaatac
     3721 tttcaatgtc tgaactgtta tattgaagca gtagccacgg ttgctgaacc tgtatggaaa
     3781 ataggctgtc acgtatcaag tccacactgt ccttgccttg actatctgaa tggggcatga
     3841 caatttttgt acccagtgac aaactagcgt tgctgatgtc tgatgaaaag tcattgattt
     3901 ttttaatgta gtcgggagcg taggcaataa aggaagccga taggataaaa accagcacaa
     3961 aattcatgag ggcatgaatt gcctttgtgg tttctctctt tatcagtcct gtataggcaa
     4021 cataaacccc aagaaccaaa atcaagagta aaaggaatcc aacatagaaa ccctctgttg
     4081 atagtccact aggcgtaacc cctgcaaggg tctgcatatt cttgccaatg gaatctgctg
     4141 tagcggaaat gaagtctaag gaataggctt cctgtactaa gtaacccgtc gcattggaaa
     4201 cataaagact gattgtccaa ataaaattgg taatggcata tagtccatac atgacttgtt
     4261 ttccaattcc gtccgaccag ttccacggta gccagcccca gctattatcc acataaaaat
     4321 ccagttgata gttttcaagt gggtatcggc tgtattcatt tgccacattg accgtatcat
     4381 ctaccaagcc cgcagcttga accaccgttc ccagcatggc taaaagaaaa atggcaatca
     4441 caagtgtgaa agccactgtc attgccactt tacctagacg tttcagcgtc cagtttgatt
     4501 ttattctgtt taatattgat ggtttcacat ttacacctct tttcgcacag gtggtctggt
     4561 atcaaaggca tggagcagtt cttcaaatac agggtggaac tgtatcacac cgacacgacc
     4621 atataaatca ctgataaggc attgcccgtt ttccaaatca cgcaatcgct tctgattgtt
     4681 ttcgtcctct gggtctacac caaaaaaggc aagggttttt ttaatctcgt taaggtcagt
     4741 ggaacgaaag gcaaatttta agccgaggtt atttttcagt ttttcatcta agaggtcatc
     4801 tgtattttgg gtcacgaaat ataccccagc gttcatagca cgaccagcac gaaccagctt
     4861 catagatagt gttttccctt gtgccacctg taagaagctc catgcttcgt ctaagtctac
     4921 aatcttgaaa atacttcggt ctgtatggat aaagtccaac gcaaaggtac taatgacaat
     4981 cagcattgct accgatagta actccattgt ggtatattcc tcaaaggaag tttccttgtc
     5041 aggaagtacc aagtcggcaa cctgtataat gttcagttgt ttttcaaggc tgatagactg
     5101 ctctacataa ccatcactaa aaagcagatg ggcaaagtca tagtctgtaa aactttcgat
     5161 atggtcggct atgctggtac ttagtggcgt attctcaacc cgtaattctt caatcacttt
     5221 catcaaccct cgcacttcac tattggttac tgcacgaatg gcttttctaa ggattgggaa
     5281 gcgttcccca tcacgagagg aaatccccgt aaggaatgtc aaaatatcaa tagccagtga
     5341 ttcagaatct ttggtatttt tcataattac ataagggtca agtaagcctt tgtttttctc
     5401 atcagaagtc agattgacga tattgatttc atgggaaatc tctggcaagg tttctttcca
     5461 tctgccacgt tctgcttttg ggtctacaat cactgcttgt gccccataaa gcactgcata
     5521 atagacgata aggttattcg caaaggattt accaccaccc agcgaaccga caaaggcaga
     5581 cgctaacgca ttggttactg aacccttaac cccttgactg gcaagagcag gtttcagata
     5641 gacattgcgt ccagtatcta agctgtagcc aacataaatc ccctcatttt cacccagcat
     5701 ttgagtagca ccaaaaccta aaccagcgag gaaatcagag gtcacgtatt gaatatagtc
     5761 attcatatat cttttgctgg caggtaaaaa ttcttcatgt aagcccagca tatccccaaa
     5821 aggtcgtacc agtttcacgc tcaaatcatc atagaaatct ttcacttcat tacaacgacg
     5881 tttaagttcg tcaaggtcat ttgctgatac ccttacaaca taggacagct tatacataga
     5941 ttccttgctt tggtctaaat tggtttccag ctcgttcaca ctttccagag cttccgccac
     6001 attagagctg gtttcattat cactttgcca agcgtggtta tccaagtctt tcagttcttt
     6061 ctttttattg cggacagtag atagggcttt acgattcgct acaatttcca cattcattga
     6121 cgtatcaatc ggaaaggtaa attgctgttg ctggtagtag aagatttcag aggacgggaa
     6181 gtccagttcg ccgacaatac tgttgatggt aaagtaagct acataggcgg tttcgtcttc
     6241 ctgctggatt ttcaaatatc gctgtttttc ttccaccaga cagcgagtag gcttaatgag
     6301 gtcataggtt ttaatcagtg tttcatgttc cagctttttc tttgatagag gatactcata
     6361 ctcttcatag gcagtgcctg tctgtccgta aaggtgttca atcagatagc cgaagtcatc
     6421 cttatctaat cttcggattt tgaaacgacg ggagatttta ttttctaaaa gcttttccat
     6481 cttctgaaaa cgcaggattt catcattact catactaaca aaatcgccca tcagcttatg
     6541 gttcacatca tagacaaaat cagtaaaagc attttttgct tcaacggtaa gacttttcat
     6601 agaaaactcc tgatcgttga gaagcaactt aaaaccgata aagaaacggt agtccacttg
     6661 attttcgcca atcatggata ttaaagcgtc tgtctgttgg tcgattttgt cataggcaac
     6721 cgttttgagc tttccagtga cttcattttt ggaacgttct tgtgcagaaa gtatgctgga
     6781 ttctgtactg atttgtaaag catgaatctt gccatcacga ttttgtgcga taagctgtct
     6841 gaaagaatca tgtacttgta ttttctgttc cggacttaga aatgagtaat tgtagggaac
     6901 aagctcatag taagcgtaac attccccatc tttattccag accagattgt tttcaatata
     6961 tttaattgga tatgccataa aattcactcc taactgctgt aatggcttct tgtggctggt
     7021 ttctgccaag cgttactttt tttcctgcat aggtcagctt tcgtcgcagt gcataagcaa
     7081 tgacagactt caaaaatcca taaggctttt taccatcaaa ggtttttgta gacataaacc
     7141 atgtgaaagc cacaggaatc ccaaagtatt tgagaaatgc tccctctatc atagaaagag
     7201 ggggcaagtt gccaagtatc ataactgcaa agagtgacac gacaaaccat gtcatttgcg
     7261 taaaggttat gggaaacgga agtctaaagt cattgatgga gtacaacact ttctcaacag
     7321 accagatact ggtatagctt cgtattttct tcatgtaatc aatcctttca taaaaatggg
     7381 gatagtcttt tgactacccc gtaaacaaga aaatctgcca gtaagaatgt accgacagat
     7441 ttaatagacg atttcaaaaa tcccgtgatt ggttgagata aacgttcccg aaaggtctaa
     7501 atcccgacca taggattcat aatcaatata gttttgaaga ctggctggca cttcgcctaa
     7561 agcacccgtt tcttcaatgt agtagcgtgc cacgtcagac atatcatcac aatcggaatg
     7621 aatgataata tcttcttgat gttcgcttag ttcttcaata cttgaaaaat gagtgagcag
     7681 agcagatagc tccgattgta gttcttctgg caactccgat accatttccc ataggcgatt
     7741 gagttcgcta atagaagtgt attcgtcaac cgtaaagggt aactcgtaat catgaatggc
     7801 gtattcctca tattcatcat tcaagccgat tttctctttg acttcctcaa agtcaatggg
     7861 aaaggtaaac cacgcaccga ccaattcgcc ctcattgtat ttgcctaaat tcgcaatata
     7921 gacttgcata tcgtccatat attcacgtcc tttctttgta gagattcaaa aatccctacc
     7981 gcacttcgtt tggtgtacca ttcctttgcg gaacataaga aaaccactta tattccacaa
     8041 aagaacggtt ttatttaagc accaataatg cgattgaata gctctagtaa aatgtctttt
     8101 actccagcag cgttgaagac taagccaacc gcaataatcg caataattaa aaagccaatc
     8161 agtttgctaa actcacgctt gaagccaaga tacaagccaa tcacaacgat tgctaaaagc
     8221 accagtgatt gagcgtttga tagaaaccag ttatatatgg ataggtaagt tcctgatatt
     8281 ataaatatct caaacttttc cccccattca cacggagcgt gcgactttca ccgcactccg
     8341 cgttccatcg tgtttatatt gaaaagtatt tatgtagtag acctatatca agtttaattc
     8401 ttgattgcca gctaatactc tatatttatt tagtcttatg agctggactt tatttagctt
     8461 caatagagct atatattttt gtattgtttc ttctgtttta gcgtgtatta gacggtgtac
     8521 cgcctctttt accaaaataa gatttttata atcgtcattt ccaccagctc cagttggtac
     8581 aatatgatga caatgtattt cattagcttg ttgaaattct tccagagtta tagcacattt
     8641 tccatgttga gcagaaaata gcgaaattcg attgtctgcg tattctattg tgcgattttc
     8701 caaagataat ttcattaact ggtacatcag taatttatca acacttagat tggtgtggat
     8761 aaattttctt ccctctagag tgtagcaatt cgctaaatac acctttgcca ttggttttct
     8821 gtgttggata tatccaacag ggaaaatagg ttcgccctcg ccagcaacat accgaagcat
     8881 tttagattta ccatatcttg ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa
     8941 tctgcccctt ttgtttgtac ctaatctgtt agtgaagata gccattacag ctctattcag
     9001 tttcatacaa tccagattta cattcgttgc aatctgataa taattttgca tacctaatac
     9061 cattgaatta taaagtcttg tttcttctaa ttccgttttc ccttttgagg gagaagcaat
     9121 tttctttgcc tgtgatacca gcttgtcttt ttggtttttg aattgtttat cacttatatg
     9181 tgatttaaca actaacttct ttcttctcgg tatcatttta atcttgaaac caagaaagtc
     9241 ggagtatctt tttctaacat ttacaattct tgttttttct tgtgatactt ccaattttaa
     9301 tcgttgttca atccattgag tgattgctat tttggctttt tcagcacttt ccttgtagcg
     9361 gcagaaaact ctaaaatcat ctgcataacg aaccatatac atttccttta actttgtttt
     9421 cttcatttct ttgtaagcgt tgcttttaca tgggctaccg cttttattga tatgaaccac
     9481 atagtttttt gtaacaggat tttcctgcca ttggctttct acccaatggt ctaactcatt
     9541 aagaacaatg tttgctagta gtggggatat tattccccct tgcggagttc ctttctctgg
     9601 atatgttatt gtaccgttag tcattttgat tggagctttt aatatccttt ttaaaatgaa
     9661 tatcaagtgc ttgtcccgta ttcccatagc ccatatctgt tttatcagct tggaatggtt
     9721 cacgttgtca aagaaaccct taatatcaaa ttcaatcaca taatgtagct ggctgatttg
     9781 taggcggtta taggttgctt ttattgcatt ttcaactgac ctattaggtc taaaaccata
     9841 gctgttttca ctaaatttag cttcgcagat tggttctaat acttgtttta tacattgttg
     9901 tataagcctg tcccacatac aaggaatacc tagaggtctg gttttaccat ttggttttgg
     9961 tatttctttt cgcctcactg gcttaggtcg atacccatgt ggactgcctg caacaatgta
    10021 tcttactttt ttgacaactg tttctgctgg caatttgcca atatctttaa ttattgtacc
    10081 gtctgttcct gctgttttac tgcctgcatt tgctttgata tttcgatatg ccagtaatat
    10141 attttctctc gataggataa tatccataag atgagtgaaa atatctccat tcttactttt
    10201 ggcatataaa tcatcaaagg tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc
    10261 tacacacaat gtttcatttt tatttttagg cataaggctt cactccttta cttgaaattg
    10321 agtgagcttt tttatcttac tcgtaatcct tattgaaaca acataatact tttctaatat
    10381 atacacgact tgagggcatt cctccattct cattacaaga atttcttagg ttcgccctct
    10441 gcttttcagc attagttcaa ctgcttattg ttcttcgtca gtttattctc ttgatgagaa
    10501 cccaatacct ttcctcgttc cgataatctt atctatacat atatatcctt aggtcactag
    10561 ctataagcct gctggcttga taccgcctgt aacgttatat ggcatttcat aacctatatt
    10621 cttactttgc cacaccagct acgatttctc gtacctttgc atttctacaa agttcaaatt
    10681 tagacccgta cattcgctgt ttcgtcagtt ccttatagaa cattctaacc atagatattt
    10741 tatagacccc cgacctgttg gatacgctac ccacctctgg atagttttcg atagaagata
    10801 tatgttcatc aactattacg gtatctttca gccgtcttta ccgagcttca aacattctga
    10861 gtttcctcat acaatgcttg tcggaggctc agggcaggcg tttcagagcg ttacttcatc
    10921 attccacctg tcagattatc agttctccta ataaccaaac tttttcgttc ttttattagt
    10981 gccttagctt ttcactaagg aacgagtcgc acaaaggttt tgtccaaaat tcataaaaat
    11041 gttctcctct ctatattcaa tgaatttgta tttgagttat ttttttgttg ttatcacgtc
    11101 ctgttctttt actgactgtt gcttcaaaat ctgcttgtgt cggtctgtca gtttcgcatg
    11161 gtcgagaatg tcttttacaa cctgcgtctg gttgatttca tcaagtttaa tcgcaacctt
    11221 taaggtcggg gcaacttgat gagatagcca gttcagcgtc ctttggaagg agtaaggctc
    11281 tggttttgtg gttagtttta atcgttcacg attgttccca ataaaccaag cccattcttc
    11341 attcagtttc caatcagaac gaggtttgga atcgtcttta tctacaaaac ggatataccg
    11401 attgataatt ttaaaggcgg tatgctctgg attgtcatag acgagtaaat cacggactgc
    11461 ataataggca cgctcatttt tcaatcgaat ctcaaaacgg ttttttactt ctgcgtcttc
    11521 aatgggaata tcatttttct tgtactgctc gtagtccttt tcatagatac agaaataaac
    11581 ttcactttgt aatgaaccga tatagagggt gtttcccata cattcctttt cctctttgcg
    11641 taccagttcg ccactgcgat agcttttaaa actgcggaag acggagatac attcttcctg
    11701 ttggcacttt tcagtgagta cagggatatt taaaatccct gtcttatcgt taatggcaag
    11761 gtcaaggcgt ttcatcacac cgccagccac caaaacgtcc ataaagaact cataccagct
    11821 tctttgttgt gccagaagat agctttcaaa ttgtctgcac ccacgacctt tcaattccac
    11881 cagaactcct ttgtccagtt catgggagca aaggacgaat atgtcgccta aagcataatg
    11941 ctctgaataa gaatagaaac catagtcctc atgaagaaaa taggacagtt tcagttgtaa
    12001 gatgttttcg accacctgct gtacgtctgt tgtcggaaag cgaattctta cataatcaaa
    12061 cagcatttca aggggagcgt cgggattgaa gcgttccaga gcttcccaaa gggactgctg
    12121 taaatcctct gatggcttga cttttcctgt ttcaatatcg cttagatact gccttgtaat
    12181 accagtcgca acagctaaac ggttttgaga tagtccataa gccaagcgtt tttcttttaa
    12241 atgctgtaac caagtttgtt cattcagtaa aaatccctcc aatcaaaaag gcgtatgtca
    12301 acttttaaag cccatttgac atacgctgaa attttgtaaa tcccttgtaa ccaaaggatt
    12361 ttctaatgtt tttttgactg tttcctgtcg atttgtaccc ccctgttaga taccgagggg
    12421 ttaagtgctg gcgtggctat tgccacacca gccagcaaga tcagtccaca cctgcgactt
    12481 ccgcttcgca cgtcgcctgc gtggactgtc tgctgttgga taacttttta atttcctcca
    12541 agaaatcata tccttttggt acaaggggag tataaaactc tgatatgaca cttgttccta
    12601 catcaacata gccacgacct ttgattcgct ttaagaagaa atccttttgt acgtcactgc
    12661 caaacatcat gccatagccc atttcagaca tacgacctaa agccactctg aaattaaact
    12721 gatcacggat tccgtcgcct aaatattttg cgtctggacg ttgacaagcc agtattagaa
    12781 agaagccagc ttgacgacct aacatgacaa tctgtttcag cttattcata actgcggtgt
    12841 tttcttttgt tcccagcatt tccatgaaag cgacgtattc atcaaagatt aagaagtgtg
    12901 ccgggagacc taagtaagca taatttttgc cagtcttata gttcttcatc tgcttcattt
    12961 cctcactacg tttcatcatt tcttcataga atgtttcaat gcaagaaagc aagtcttctt
    13021 ttctatagta gacatttgcc atcacagaac ctaagtccgc aaggtcagca tttttcgggt
    13081 caagaatata cagttttgaa tctgtatgaa gcaaggcttc aatcagtgtc agtataaagt
    13141 aagttttacc gccacctgta ccaccagcaa tcaacatatg agggagctta tcatattccc
    13201 accatacgtt tttcattaag cgaagtttac catctttagc ttctacttca tcaatagaaa
    13261 tacgactggc tatggtgtca tagagcaaag tatattccac ataggaatcc tttaactctt
    13321 tatccgtcag ctcacagtac aagccactct ctaatttctt ttccaagtgt aagagttggt
    13381 cttgatattt tcccagcgtg atttccaccc gtatctgtat caagccattt ttaagtcgat
    13441 aatacatttt agggaagtag gttatctttt cctttgtacg accagcacta tctttaaaga
    13501 aaccctctgt tttgacctgt tcagattcat accacttgtt ttcaagtatc atctttgcca
    13561 gtttttgacg gtggtaaagt tgtttaaccg tatcatagcg aacccgtttg aatacaaacg
    13621 ctaccagcaa gcagataaga attgcgacac tgaaactgat aattaaatag ggaatgtcaa
    13681 tcttatctgc ttgtgatagg ttaaaatcct gccagttgat ctgctggatt gtcttcacat
    13741 gaaacagtcc gacaaccagc aggaaaacag gcaggagtga cgctatcgta aaatgaaaga
    13801 ctaaatcttt accagatggg cgaatccttt taccacgctg tttcatgcga aaaagtctcc
    13861 tttctaccta gcgactattt gtcttgtgtc ggttctttct ttgcttgtgg ttgagctttg
    13921 aatgaactag aatcctttgt cagcacaata tcgtctgcct tgatatacca gtcaacatct
    13981 gctccttgat aggtggcagt agcaacggtg tccgcaatgg gattgataag ttccacccgt
    14041 gcgttataat caaactcttt caaaggcacg ctggcaggaa tacttacttg aatcatgcgt
    14101 ccttgtcctt tggattttaa gtcataggta cgttccttga tttcatctga aaccgacccg
    14161 tcttcatttt ggattctcac ttcacgacgt agagcagaga atttcaattc tccaaaagtc
    14221 gtgtctttat ctaatacaat gccatttgct aatctcatca tttttcctct ctttctttat
    14281 tcttttatca tgtcgtcagc atgtaaaagg taatttgtaa aaccacgagt gccgattttg
    14341 tagccctctg cggtaatacg tggattgact aacttcacac gttcctcaaa gccgaaatgt
    14401 ttttcgccag cttcagcagg aagcaccacc acaatatcat ctgctctttg aacatcagaa
    14461 tagagattat agcttcttga taagacagtt agccgtccgt tgattcttcg ctgaacgact
    14521 ttatcctcgc cagcaaattc taaattgccg aatgtttttt ccatgttggg aatcacaaat
    14581 ttaagttcca tatttttacc tatcctttct tttttattgg ctgaatgaat gtttgatggt
    14641 cttaaagagt ggggaacgac cttttgattc ttgatttttt gttttcataa gttcacttcc
    14701 tttcaaaatc gggtaaaaaa atagacacct cattttttga agtgtctacc tattaaatat
    14761 tcaaatttta ttggaagtat ctttatatct tcacttttca aggataaatc gtcgtatcaa
    14821 agctcattca taagtagtaa attagtagta aattgagtgg ttttgacctt gataaagtgt
    14881 gataagtcca gtttttatgc ggataactag atttttatgc tatttttctc ttaaaaaata
    14941 tcattatttg tggtatggag cccccgcgtt gatccggaag gcacggtaga tttgctcgac
    15001 gaggatcagg cgcatcaatt gatgtggata cgtcattttg ccaaaagaga tctgagcatt
    15061 gcttcttttc atgacggctt cgctgagtcc taaagaaccg ccgataacaa aaacaaggtg
    15121 gctttttcct tggataccta attggtcgat ttgtttagca aaagattcgc ttgtgggatt
    15181 tttcccttca attgctaagc catagacgta ttcttgttct ttgattttcg ctaagatccg
    15241 ttctccttct ttttctttta cttgtctcat ttgcgcttcg cttaaattct ccggggcttt
    15301 ttcgtcagga acttcgatca attcgatttt cgcataggca ttcagccgtt tgacatactc
    15361 attgatacct tggatcaagt atttttcttt taattttcct actgaaataa ttttgatatt
    15421 catgttttct ctcctttgat cttcacgctc actttgttta ttataatcaa ttttttcgtc
    15481 tatatgttta ttttgactat ttttgttaaa aagatttccc caacagttat gcacaacatc
    15541 cacaggttta tccacagaaa acagcgttgt tttaccttat ttcatcacat tcattaggca
    15601 aacatacgtt ttataagaat ttatccactt tatacacagg ctgtggatat gttttatccc
    15661 ttatttttcc aatcctatcc gcgtagtttt acacaatatc tgtggaaaaa gaaaatcaaa
    15721 tattttcatc tgaaaacaat gatttttcct aaagtttttc ataaaaagtt caaagtctca
    15781 aagtagacta aagtcaacaa ataagactat actaacagta gataaatgaa tgtaattgga
    15841 ggaggaacac atggatagaa aaaatgtgac accaaagatg aaaaaaaata aaaataatag
    15901 cctctggcgt aagttgggtc tggggcttgt tggagggatc gttggaggac tactgacagc
    15961 tggcattttt tatgcagcga tgggcactgg caacaatagt tctactgcta caagcggcaa
    16021 tcaaaattct gccggtgaaa cagttgtgga aaatgtcaaa gtcaatgtag attctgatat
    16081 caccaaagcg gtagataaag ttcaagacgc tgtcgtttct gttatcaact tacaaagtca
    16141 aaaccaatca agcggttttg gcggattatt cgggcagcaa gaagaaagta gcagctcaga
    16201 tgatagtaat ttggaagctt atagtgaagg aagcggcgta atctacaaaa aagacgggaa
    16261 tactgcttat gtagtcacaa acaatcacgt agtagatgga cagcaaggtt tggaagtcat
    16321 gatgaaagac ggtacgaagg taaaagctga attagttgga acagatgcct attcagattt
    16381 agcggtgttg aagatcaatt cagacaaagt cgaaacggtt gcctcttttg gggactcaag
    16441 tgctttgaag gttggtgaac ctgcgattgc gattggttcc ccattaggat ctgaatatgc
    16501 gaattctgtc acttcaggaa tcatttcttc cttgaaccga caagtaacaa gtacgaatga
    16561 atcgaacgaa acagtcaata tcaatgcgat ccaaacagat gcagcgatca acccgggaaa
    16621 ttctggtggt cctttagtga atatcgaagg acaagttatc ggtatcaact caagtaagat
    16681 cgcaagtact tctgaatcga cttctaatgt aagcgtagaa ggaatgggat ttgccatccc
    16741 aagtaacgat gtagtgaata tcatcaacca actggaaaaa gacggaaaag tgacacgtcc
    16801 agctctaggt atcacaatgg tcgacttatc tgccgtttct actcagcaac aagaacaaat
    16861 cttgaagatt ccttcatctg taactaacgg tgtcatcgtg acaaatgtcc aaacagctac
    16921 accggcagaa aaagctgggt tgaaacaata cgacgtcatt actaagatcg atgacaaaga
    16981 agtttcttct ggtgtagaac ttcaatctgc tttgtataag aaaaaagtgg gagattctgt
    17041 caaaatcact tactatgaag gctcgaaaga aaaaacaact actgttgaat tgacgatcga
    17101 tcaatcggca ttaaaacaaa atcaaaatag tgaaaattaa taaaaaagag gttgtgacaa
    17161 agacttgtca caacctcttt attttggaaa tacttctccg actacggcat cctcaggcaa
    17221 tactaagatt cctttttttt ctggagcatt tggcaaatgc aattcacgag cagaacaaat
    17281 cattccatag ctggctaccc ctcgcaaagt tcctggccag atcatcattc catctggcat
    17341 catggctcct ggttttgcaa cgacaacttt ttgtccagct tcgatatttg gtgcaccgca
    17401 aacaatttgc aatactgtcc cattgtccac ttctgtctca gtaatcgata aatggtcaga
    17461 gtcaggatgc gggacgcatg tttttacata cccgacaacg atcttcggtt catgatcagc
    17521 ttcaagctgt tcttcgaatc ctgcttcttt aatcaatcga ttcaatttcg ctaattcttc
    17581 atctgttaag cttacttggc cgttcccttc aattgttaga tgatcggaga tatggaaaaa
    17641 gttccaagca acaacttgac cttgttcatc tttgatccga gcaacattcc atttacgttc
    17701 gacagtgttt tctgttcctt tatcgtcggc tatgataacc atcaaagtgt ctccgacgtg
    17761 tgctttgtta taagcaaaaa tcatgttctt tcctccatta tcctttgctg agatttatct
    17821 taacaaaaaa ttgcattcac tgcatgagat ttttttcatg ctttatttcc gaagaagctt
    17881 cttctggaac accatttatt cgtaacaagg gactgtgaca agacttttgt cacagtccct
    17941 ttactgtttg attaaaaagg ttatacaagt tccacttgct taattactcg gctgttcttc
    18001 ttcgtcagaa acgatcaaga catcacaagg tgaatgacgt atgacgtagc tggctacact
    18061 tcctgtcata aagcgttcga ctgcatttag tccagactgc ccaaccatga ttagatcgat
    18121 tgcatatttt tttggcaatt tgactgccat ggattcacga acagagccgt atgcaataat
    18181 tccttctata tttgtgtaat caacagattt tccatattct ttacattctt cgatcatttg
    18241 cttggctgtt tcagtttgct gatcgactag attttgatca ataggagcat agcccataaa
    18301 attataaaat tgctgatcga ttacgtttgc tacatatact ttcccattat tacggcgagc
    18361 cacttctaac gcttttttga aagcagcaag tgcttgttca ctaccgtcaa tacctactaa
    18421 aatgttctta tattcttgat tcatcttgac ccctcctaac agatgtcagc cttttattac
    18481 actttctagg aacgattcga tttcctgttt tgttttacga tctttattca ctagacgacc
    18541 taattctttt ccgtcttgga tcacgacaaa acttgggata ccaaaaatgt tccattcagc
    18601 ggctacatct atgtacttgt cacggtctac ttcaataaat tgccaatcag aaaagtcatt
    18661 ttcgatttca ggcatttgcg gtttgataaa acggcagtct ccgcaccagc cggcagtaaa
    18721 gaaaaataca ctttttccag tttctacgta gcctgctaat tcttctaaac tatttgggat
    18781 aatcattctt tttcctctac tttctgttta tgtatttttc aattaaatta tacacctttc
    18841 tttcgaacta acaaacattg atcctctttt tataaaaaaa atctgacaaa aggttgcttt
    18901 tgccagattt tatagtttca ctatcgcgtt atttcccgta tacgatcgtg ttcactgttg
    18961 atttatctaa acctttcaat gtttgaatca acatttctcg tgcagcttca aaatcgcgaa
    19021 tactgaacat cgtttgatgc gtatggatat aacgtcccac tacgccaatc actgtacttg
    19081 ggattccttc attttgtgta tgagcagcac ctgcatctgt accgcctttt gaaacaaagt
    19141 attgataagg gatgttgttt gtttcagcga tatctagcaa gtactcacgc aaacgaggaa
    19201 gcatgatcaa acctggatct tggatacgca tcaatgtacc ttctcctaaa tgtccgaatg
    19261 tgccattttt cgtcactgtg tcatctgctg cagagcagtc tactgcaaag aacagatctg
    19321 gcttgaattt attgacagaa gctttagaac cacgcaagcc tacttcttct tggacattcg
    19381 cgcctgcaat caatgtatga cctaatgttt cattttgtaa cgcttctaaa gcttctaata
    19441 ccattgtaca gccatagcgg ttgtcccacg atttgctgat gatattcttc ccgttggctg
    19501 ttttgatcgt ttcagcgtaa ggaacgattg tgtctcctgg aagaacaccg taactcattg
    19561 cctcttctct tgattcaaac ccagcatcga acaaaatatc tgttacttct actgattttt
    19621 gtccgcttgt tcctcgtaat aaatgtgggg gaacggaaga agaaatacat ggataattct
    19681 taccagtaga ggttttaagt gtaaaacgtt gagcagaaac aacgtaaggg ttccatccgc
    19741 ctagaggtac gactttaaaa agcccgtttt cttggatttg tgtcagcatg aatccgactt
    19801 catccatatg tgctgcgacc attacacgag gtgcatccgc ttcttcatgg tggcgcagcc
    19861 caaaaatgcc gcccaaacca tctaactgta actcatctac cagcggttcc atatgacttt
    19921 ccatataggc gcgaacgtct tgttcaaaac cgcttgttcc ttgtaattct gttaactctt
    19981 tgattcgttg aaatgttttt tcttccatat gctattcgtc taaatttcag actaaactcc
    20041 tctcagactt atattgatct gcggataaaa tatgattttt tctgcagacc gatcgtattt
    20101 attatagctt attttttctg gtagcggtta tttatttcaa agaaaatatg agagtgtggt
    20161 aaaataaaca caaagagtag caagtgagaa gaaggaggta tatccacatg tatgaagaaa
    20221 gcgaattaag ctattttaaa ggcgggttag ccgttgggat cggtctagga ttattgagtg
    20281 gggtagcttc aacgatttgg tatcatcgca aaaaagtgtt atcagcagac caagtgcttg
    20341 aaaatatcaa aacatctttt ttaaaagaag gcgcaatcga aggtgcttgg atctcttttg
    20401 aaaaagaacc tattcggaaa ttcgccgttc ggtcgagtgc ttatcggggt gggctttccc
    20461 ggatagaaga tggcgaaact gtttattatg aatttttagc agatgctcat acaggaaccg
    20521 tattagacat ttcaagaaaa aagggcgcaa tagattaata cgtcaaaaaa atgaaataac
    20581 gaatacgcac ccactatcta aaagagaggt atcctatcct tcttttatag tgggtgattt
    20641 ttataaaaat agggctattt cctcttggaa tagctagtat ttgtagttat tatacggtat
    20701 tcttgaaata agattagttt tattatataa ggagagtgac aaatggaatc acgtgaaaaa
    20761 attgccgtgc tcattccttg ttataacgaa gaggcgacaa tttcaactgt tattgaagat
    20821 tttaaaaggg aattaccaga agcagatatt tatgtctatg ataataattc gaccgatcga
    20881 acatacgaat tggccgttca aggcggagca atcgtaaaga aagaaccacg tcaaggaaaa
    20941 gggaatgtaa tccgtcaaat gttttttgat attgatgccg attattattt gatggtagat
    21001 ggtgatgata cttatcctgc agaagctgca catggtttgc ttgagaaatt acgttctggc
    21061 gaagcagata tggtcattgg cgatcgtcta tcaaatggaa cctattttga tgaaaataag
    21121 cgtcctttcc atgattttgg gaataatctg gttaaaaata caattactta cttatataaa
    21181 acaaaaattc gcgatgtcat gaccggctat cgaggattca atcgaatttt tgtcaaaagc
    21241 ttccctatta tgagttctgg ttttcaaatc gaaacagaat tgacgattca tgctttagat
    21301 aaaaaattca agttcgttga gattccaatc gattatcgcg atcgtcctga aggtagcgaa
    21361 tcaaaattga atacattctc tgacggattc aaagtgatca tgatgatcat caaaatgtgc
    21421 aaagattata aacctttgct tttctttggg atctggacca tcttcttctt tttatttgga
    21481 ttggtcatgg ggattcctgt gattcgtgaa tatatgctga caagctttat tacacgcatc
    21541 ccttctgcga ttttaagtac tggattgatg actttagccc ttctttcatt agtaacaggg
    21601 ttgattttgg atacggtcgt cacaaatgcc aagaaagaat atgaattgaa tttataccat
    21661 gtctaccacg aatatcaaaa taaagaaatg aagtaggagg ccttttttat gcataagaaa
    21721 aaggagaatc tatttcctac tttttttgca actttatttg cgtcaggttt tgcttttttt
    21781 acttcttatg gtctaggaac tttgaatcca acagtaatca ttggtttagc catggttttg
    21841 ctacttgctt ttaagcaata tggaatagag ttgttttcaa taaaaaacaa agttgcagct
    21901 attgcccgat tgtttttttc tgcagctttt gctcttacac tttctattgg cagacagata
    21961 caatttcagt atgtctcagc aaactataca aagacaacga ttgctgatgg aagtattttt
    22021 tactttcttg tttatttctc catttttgtg tattcttttt tatttttaca atcgatttat
    22081 ttatcaatgc caaaactgga aacatttttt actgctcaaa acacacaggc tttctcttca
    22141 agaaaatatt ttttattctt tactgtcttt ttagttctgt gttggcttcc ttatctgttg
    22201 gcattgtatc ctggagtggt tttgcctgat tcgttaagtt cggtcgccca atctatgggt
    22261 gatgttccta taagcaatca tcatccagtc ttattcacct tactggtcaa gctatttgtt
    22321 catgatttac caactgatac gatcaatcac ggtgtatttt tattttcgat ttttcaaaca
    22381 ttgataacag cttcagcgat aagttattct ctttgctggc tgttaaaaag aaacatccat
    22441 ttttttccgg tactcattag tttatgctat tttgcatttg caccggtttt tccaatctac
    22501 gccttgaaca tgcaaaaaga tactttgttc tctatcgctt gctttttatt ttccttagcg
    22561 gtttttgact ttatttttca acgagaaaag ccaacgattc tcaatcagat ttacgtaatc
    22621 atccttgctt tactgaccac ctatctaaga aataatggga tttatgtagt aaccggcaca
    22681 ggtttgattt gcggcttgat tattttgaaa caaaaaaggt atcaagcatt ctttcaagtg
    22741 gtgctgggtt atacagtgat tgctggacta ctcatccacc cgttgatggc tcgttatgct
    22801 attcctacag aaactgctga acaattaggt gttcctttgc agcaaatttc tagaactatt
    22861 gttatggatg gtgacttgtc taagaaagat aaagcattca tgaatcagct actcccgata
    22921 aaagattatg aagcgtattc tcctagtctt gcagactcaa tcaagtggca taaaaatttt
    22981 aacaatgagt ttttgaatac acataaaaaa gaatttctat cactatgggc aagaaattta
    23041 ccaagtaata taaaaatcta tacagatgct tatattttag aaacgttcgg gttctgggta
    23101 cctggtacaa aaaatagtta cggcttttta gatactcgtg taaacacgaa taattatggt
    23161 atgaagcaag tagatttgat tgaacatctt actggattgt caggaatgaa aaaaatcatc
    23221 gatgctcgtg atttctttgg ttccggcaca ttactgtggt tagctatcgg cagtgcactt
    23281 ttagcaatct ttaagaaaaa atatcggtat ttactccttt ttcttcccgg tatgcttgtt
    23341 tttgctacta ttatgattgc tactcctgtg gcttttagtt tacggtatgt gtttgtttta
    23401 gcattaggtc tacctgttta tatcctctct ccttttttac tagataaaag agaaaaaatg
    23461 aaaacagata taggagatac tcatgaaact atttaaaaag atattttttg cttttgttct
    23521 cttgatcgct agctattgcg tactactgac aattactttt ttcattccgc agcactctat
    23581 tgaatcgaat gcagaaaaat ctctacagat ggttgaaaaa gaaggaatgt atccttctat
    23641 caatcaagga aatatgctag gcactcggtt agataatttt actgatcatt tgatgatacg
    23701 aaaaacaaaa gcagatcctg aattgaactc cttggagaac gcgatgtcta tggcggatta
    23761 tccccgttat tggcatggtt atcagctttt tttacgacct ttactgctag ggatgagttt
    23821 ggggagtatt cgaatgattt acgcagccat tctttttctg ctgattggat tgacgagcta
    23881 ctttttaacc aagtgtagcg acatctattt aacgacagcc ttgttgattt cgcttgctat
    23941 aggaaatgct gcaacctttt tcttcagtat gcaattttca aatatttgga ttttgacttt
    24001 attgtctgta ttactatttt taacaaaacc acaactctcc aacaccaacc aacaattatt
    24061 cttgtatttc tttgtcattg gttctttagc taactttttt gacttgttga caacaccggt
    24121 catctcttgg gggatacctg tgatcattat ttattatgta acaaataagt atttaatgga
    24181 taaacgcagt tcattgagca aacaagtagg cagttttgtt tttaccggta tcttttgggg
    24241 attaggctat ggtttgacat gggtggctaa atggatccta tcatcgatca tattgaacaa
    24301 aaacattgta aaagatgcaa tcaaccaaat cctctttcga acggaaggaa acgacaaata
    24361 cccgttgcat cgattagaga tgctgaaaag caatattaga ttgatgtatc caaaagtagc
    24421 aatattattg ctattgatta cttgtcttgt ttttctctat tttgcttatc gtaaacgaaa
    24481 tagctggcgt ccgttccaac ttctagtttt gtttgtttta gctgctgtaa cgccgtatat
    24541 ttggtacaat attttagcta atcactcaca gattcattat tggtttacgt atcgtacgca
    24601 aatcatcaca agttttgcta tacttagtgc tttcgccttt ttgatccctc ctgagaaatc
    24661 aaaagacgaa ctaaattact tttaagaata ccgatgtact aattcatttt taaacataaa
    24721 aactccagct gtcagagata gccggag
//
LOCUS       DACDYM010000037        24577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDYM010000037 DACDYM010000000
VERSION     DACDYM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.037"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(152..550)
                     /locus_tag="IWM59_001511"
     CDS             complement(152..550)
                     /locus_tag="IWM59_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9979549.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="IWM59_001512"
     CDS             complement(547..1320)
                     /locus_tag="IWM59_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9979550.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="IWM59_001513"
     CDS             complement(1317..2000)
                     /locus_tag="IWM59_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9979551.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="IWM59_001514"
     CDS             complement(2167..3054)
                     /locus_tag="IWM59_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9979552.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="IWM59_001515"
     CDS             complement(3051..3920)
                     /locus_tag="IWM59_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9979553.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="IWM59_001516"
     CDS             complement(4057..5343)
                     /locus_tag="IWM59_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9979554.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="IWM59_001517"
     CDS             5616..6194
                     /locus_tag="IWM59_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9979555.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="IWM59_001518"
     CDS             6194..7123
                     /locus_tag="IWM59_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9979556.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="IWM59_001519"
     CDS             7120..8856
                     /locus_tag="IWM59_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9979557.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IWM59_001520"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IWM59_001520"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9979558.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="IWM59_001521"
     CDS             complement(9752..10261)
                     /locus_tag="IWM59_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9979559.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IWM59_001522"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IWM59_001522"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9979560.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="IWM59_001523"
     CDS             complement(11562..12653)
                     /locus_tag="IWM59_001523"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9979561.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IWM59_001524"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IWM59_001524"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9979562.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IWM59_001525"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IWM59_001525"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9979563.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IWM59_001526"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IWM59_001526"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9979564.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="IWM59_001527"
     CDS             complement(16616..17590)
                     /locus_tag="IWM59_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9979565.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="IWM59_001528"
     CDS             complement(17571..19454)
                     /locus_tag="IWM59_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9979566.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="IWM59_001529"
     CDS             complement(19472..21697)
                     /locus_tag="IWM59_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9979567.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="IWM59_001530"
     CDS             21812..22846
                     /locus_tag="IWM59_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9979568.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="IWM59_001531"
     CDS             complement(22913..23926)
                     /locus_tag="IWM59_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9979569.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="IWM59_001532"
     CDS             complement(24004..24192)
                     /locus_tag="IWM59_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9979570.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7063 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aactaaa
//
LOCUS       DACDYM010000038        24271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDYM010000038 DACDYM010000000
VERSION     DACDYM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.038"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..572
                     /locus_tag="IWM59_001533"
     CDS             <1..572
                     /locus_tag="IWM59_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9979571.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="IWM59_001534"
     CDS             576..1217
                     /locus_tag="IWM59_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979572.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="IWM59_001535"
     CDS             1214..2131
                     /locus_tag="IWM59_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9979573.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="IWM59_001536"
     CDS             2128..2799
                     /locus_tag="IWM59_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979574.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="IWM59_001537"
     CDS             complement(3340..3828)
                     /locus_tag="IWM59_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9979575.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="IWM59_001538"
     CDS             complement(4029..4295)
                     /locus_tag="IWM59_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9979576.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="IWM59_001539"
     CDS             complement(4295..4558)
                     /locus_tag="IWM59_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9979577.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4551..4823)
                     /locus_tag="IWM59_001540"
     CDS             complement(4551..4823)
                     /locus_tag="IWM59_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979578.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5034..5219
                     /locus_tag="IWM59_001541"
     CDS             5034..5219
                     /locus_tag="IWM59_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9979579.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="IWM59_001542"
     CDS             complement(5361..7184)
                     /locus_tag="IWM59_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9979580.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="IWM59_001543"
     CDS             complement(7782..10487)
                     /locus_tag="IWM59_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9979581.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="IWM59_001544"
     CDS             complement(10793..11740)
                     /locus_tag="IWM59_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9979582.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="IWM59_001545"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="IWM59_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9979583.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLHKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="IWM59_001546"
     CDS             complement(12375..12737)
                     /locus_tag="IWM59_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9979584.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="IWM59_001547"
     CDS             complement(12748..13869)
                     /locus_tag="IWM59_001547"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9979585.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="IWM59_001548"
     CDS             complement(13885..14235)
                     /locus_tag="IWM59_001548"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9979586.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="IWM59_001549"
     CDS             complement(14365..15876)
                     /locus_tag="IWM59_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9979587.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="IWM59_001550"
     CDS             complement(16198..17550)
                     /locus_tag="IWM59_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9979588.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="IWM59_001551"
     CDS             complement(17703..18779)
                     /locus_tag="IWM59_001551"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9979589.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="IWM59_001552"
     CDS             18975..20720
                     /locus_tag="IWM59_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9979590.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="IWM59_001553"
     CDS             complement(21000..21437)
                     /locus_tag="IWM59_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9979591.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="IWM59_001554"
     CDS             complement(21421..22794)
                     /locus_tag="IWM59_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979592.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="IWM59_001555"
     CDS             complement(23116..23604)
                     /locus_tag="IWM59_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9979593.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="IWM59_001556"
     CDS             complement(23626..24228)
                     /locus_tag="IWM59_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9979594.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6960 a         5163 c         4487 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcat aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DACDYM010000039        23827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDYM010000039 DACDYM010000000
VERSION     DACDYM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.039"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(53..787)
                     /locus_tag="IWM59_001557"
     CDS             complement(53..787)
                     /locus_tag="IWM59_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9979595.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IWM59_001558"
     CDS             1114..1524
                     /locus_tag="IWM59_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9979596.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IWM59_001559"
     CDS             1517..2218
                     /locus_tag="IWM59_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9979597.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IWM59_001560"
     CDS             2218..3804
                     /locus_tag="IWM59_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9979598.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IWM59_001561"
     CDS             3823..4044
                     /locus_tag="IWM59_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9979599.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IWM59_001562"
     CDS             4041..4802
                     /locus_tag="IWM59_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9979600.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IWM59_001563"
     CDS             5182..5691
                     /locus_tag="IWM59_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9979601.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IWM59_001564"
     CDS             complement(5766..6305)
                     /locus_tag="IWM59_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9979602.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IWM59_001565"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IWM59_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9979603.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IWM59_001566"
     CDS             7372..8169
                     /locus_tag="IWM59_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9979604.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IWM59_001567"
     CDS             8197..8832
                     /locus_tag="IWM59_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9979605.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IWM59_001568"
     CDS             8852..9625
                     /locus_tag="IWM59_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9979606.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IWM59_001569"
     CDS             10033..10830
                     /locus_tag="IWM59_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9979607.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IWM59_001570"
     CDS             10808..11221
                     /locus_tag="IWM59_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979608.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IWM59_001571"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IWM59_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9979609.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IWM59_001572"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IWM59_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9979610.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IWM59_001573"
     CDS             15998..16558
                     /locus_tag="IWM59_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9979611.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IWM59_001574"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IWM59_001574"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9979612.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IWM59_001575"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IWM59_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9979613.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IWM59_001576"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IWM59_001576"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9979614.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IWM59_001577"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IWM59_001577"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9979615.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IWM59_001578"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IWM59_001578"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9979616.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IWM59_001579"
     CDS             21905..22483
                     /locus_tag="IWM59_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9979617.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IWM59_001580"
     CDS             22535..23017
                     /locus_tag="IWM59_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9979618.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IWM59_001581"
     CDS             23134..23508
                     /locus_tag="IWM59_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979619.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atagaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DACDYM010000040        23677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDYM010000040 DACDYM010000000
VERSION     DACDYM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.040"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            164..2698
                     /gene="secA"
                     /locus_tag="IWM59_001582"
     CDS             164..2698
                     /gene="secA"
                     /locus_tag="IWM59_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9979620.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            2825..3926
                     /gene="prfB"
                     /locus_tag="IWM59_001583"
     CDS             join(2825..2887,2889..3926)
                     /gene="prfB"
                     /locus_tag="IWM59_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9979621.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4031..4717
                     /gene="ftsE"
                     /locus_tag="IWM59_001584"
     CDS             4031..4717
                     /gene="ftsE"
                     /locus_tag="IWM59_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9979622.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4701..5594
                     /locus_tag="IWM59_001585"
     CDS             4701..5594
                     /locus_tag="IWM59_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979623.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5746..7194
                     /gene="cls"
                     /locus_tag="IWM59_001586"
     CDS             5746..7194
                     /gene="cls"
                     /locus_tag="IWM59_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9979624.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            7531..8388
                     /locus_tag="IWM59_001587"
     CDS             7531..8388
                     /locus_tag="IWM59_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9979625.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8503..9423
                     /gene="pstC"
                     /locus_tag="IWM59_001588"
     CDS             8503..9423
                     /gene="pstC"
                     /locus_tag="IWM59_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9979626.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9423..10307
                     /gene="pstA"
                     /locus_tag="IWM59_001589"
     CDS             9423..10307
                     /gene="pstA"
                     /locus_tag="IWM59_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9979627.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10322..11128
                     /gene="pstB"
                     /locus_tag="IWM59_001590"
     CDS             10322..11128
                     /gene="pstB"
                     /locus_tag="IWM59_001590"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9979628.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11144..11902
                     /locus_tag="IWM59_001591"
     CDS             11144..11902
                     /locus_tag="IWM59_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9979629.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11915..12592
                     /gene="phoU"
                     /locus_tag="IWM59_001592"
     CDS             11915..12592
                     /gene="phoU"
                     /locus_tag="IWM59_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9979630.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12814..13848
                     /locus_tag="IWM59_001593"
     CDS             12814..13848
                     /locus_tag="IWM59_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9979631.1"
                     /translation="MQKKISIIITLFFICILASWFIQRQAALYDVPVIEIERVRIEED
                     QQLITGRLMNTDQKGEVISLTAPYQKNQIETANITTRQIYLVQMHGDEWQLVAKKNDG
                     WLFFFTSVFIGTMLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAILFV
                     VIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTR
                     PYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVIGS
                     MANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIAVL
                     FFLAVRRLKK"
     gene            13845..14612
                     /locus_tag="IWM59_001594"
     CDS             13845..14612
                     /locus_tag="IWM59_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9979632.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            14712..16280
                     /gene="liaX"
                     /locus_tag="IWM59_001595"
     CDS             14712..16280
                     /gene="liaX"
                     /locus_tag="IWM59_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9979633.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16459..16764
                     /locus_tag="IWM59_001596"
     CDS             16459..16764
                     /locus_tag="IWM59_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9979634.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            16764..17114
                     /locus_tag="IWM59_001597"
     CDS             16764..17114
                     /locus_tag="IWM59_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9979635.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            17280..18218
                     /locus_tag="IWM59_001598"
     CDS             17280..18218
                     /locus_tag="IWM59_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9979636.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18233..19063
                     /locus_tag="IWM59_001599"
     CDS             18233..19063
                     /locus_tag="IWM59_001599"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9979637.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            19107..20132
                     /locus_tag="IWM59_001600"
     CDS             19107..20132
                     /locus_tag="IWM59_001600"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9979638.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20147..21085
                     /gene="galU"
                     /locus_tag="IWM59_001601"
     CDS             20147..21085
                     /gene="galU"
                     /locus_tag="IWM59_001601"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9979639.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            21210..21659
                     /locus_tag="IWM59_001602"
     CDS             21210..21659
                     /locus_tag="IWM59_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9979640.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            21652..22254
                     /locus_tag="IWM59_001603"
     CDS             21652..22254
                     /locus_tag="IWM59_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9979641.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(22317..23420)
                     /locus_tag="IWM59_001604"
     CDS             complement(22317..23420)
                     /locus_tag="IWM59_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9979642.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
BASE COUNT         7777 a         4153 c         5190 g         6557 t
ORIGIN      
        1 ttttttgagt ttcctcctct tttagatgaa atatgaataa attatgaagt tcttacttta
       61 atacgcttaa gatatttcaa ttatctataa ctaatgataa aatgaaaagt aaagtctaca
      121 tagtctggat aatacacaga aggaaaagga gattacacaa cgcatggcca atttgattcg
      181 ttcacttatc gaaaacgata agaaagaact aaaacgttta gatagcattg caaaaaaagt
      241 tgaagcatac gctgaccaaa tggcagcatt aactgatgaa caattacagg caaaaactgc
      301 tgaattcaag ggcagatacc aaaaaggaga aacattagat caattacttc cggaagcttt
      361 tgcagtcgtc cgagaagcag caaaacgtgt attggggtta tatccatacc acgtacaatt
      421 aatgggtggg atcgtgcttc acgatgggaa tatccctgaa atgcgtactg gtgaaggtaa
      481 aaccttgact gcaacgatgc cggtgtatct gaatgcgttg actggcgaag gggtccacgt
      541 agttaccgtc aatgaatatt tagcaacccg tgactcgacg gaaatgggcg agctttataa
      601 tttcttagga ttgacagttg ggttgaatat caactcaaaa tcttcggatg aaaaacgcga
      661 ggcttataat tgcgatatca cttatagcac gaataatgaa ttaggatttg actatttgag
      721 agacaacatg gtcgtttacc gtcatcaaat ggtacaacgt ccgctgaatt atgccattgt
      781 cgatgaagtt gactcgatct tgattgatga agcgagaacg ccgctgatca tttcagggca
      841 agcagaaaaa tcaacagctt tatatacaag agcagacaac ttcgtgaaac gtttgaaaga
      901 agaagaagat tataaaatcg atatacaatc aaaaacaatc agtttaactg aagcagggat
      961 cgaaaaagct gaagaaacat tcggtttgga caacttatat gacattgaaa atactgcatt
     1021 gacccatcac ttggatcaag cattgagagc caactacatc atgcttttag acatcgatta
     1081 tgttgttcaa gatggaaaag tattgattgt tgaccaattt acgggtcgga tcatggatgg
     1141 acgccgttat tcagatggtc ttcatcaagc gatcgaagct aaagaaggcg tagagatcga
     1201 agacgaaacg aaaacgatgg cgacgatcac tttccagaac tatttccgta tgtacaagaa
     1261 acttgcgggg atgactggta cagctaagac agaagaagaa gagttcagag agatttataa
     1321 tattcaagta taccagattc cgacaaaccg tccggtcatc cgtgacgatc gaccagattt
     1381 gctttaccca acattacaaa gcaagttcca tgctgtcgta caagatatca aagatcgcta
     1441 ccgcaaagga cagcctgtct tagtcggaac tgttgcagtt gaaacatctg agttattatc
     1501 agatatgctg aatcaagaac gtattcctca tgaagtactg aatgcgaaaa atcactttaa
     1561 agaagcagaa atcatcatga atgcgggtca aaaaggtgca gtgacgattg ctaccaacat
     1621 ggctggtcgg ggaacagata tcaagctagg gcttggcgta agagaacttg gtggcctagc
     1681 agttatcggg acagaacgac atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc
     1741 aggacgtcaa ggagaccctg gtatgtcaca attctacttg tctttagaag atgatttgat
     1801 gaaacgattc ggttcagaac gaatcaaagc tttcttagat cgaatgaaga tcggtgacga
     1861 agatgctgtg atccaaagca agatgctgac gaaacaagta gaagctgcac aaaaacgcgt
     1921 agaaggaaac aactacgaca cacgtaagaa cgtcttgcaa tacgatgacg tgatgcgtga
     1981 gcaacgtgaa gtaatctata aacagcgtca agaagtgatc atggaagaaa aaagcttatc
     2041 taaaccactg atgaatatgg tcaaacggac aatcagtcgt gtggtagatg cgcatacgca
     2101 attagaaaaa gaaaattgga atttggaaag tatcgtcgat tttgctggca gcacgcttgt
     2161 ccatgaagac agtatttccc ttacagatat cgaaggaaaa acctctcaag aaatcaaaga
     2221 atatctaaac gaacgagcac aagaagtatt caataccaaa gcagctcaat tgaacggacc
     2281 agagcaactg cttgaatttg aaaaagtcgt gatcttacgt gtcgtggact ctaaatggac
     2341 agatcatatt gatgcaatgg accaattacg tcagtcaatc ggcttgcgtg cctatggtca
     2401 aaacaatcca ttagtcgaat accaaacaga aggttataag atgttcgaag atatggtagg
     2461 tgccattgaa tatgaagtca ctcgtatctt catgaaagct gagatccgtc aaaacgtaca
     2521 acgtgagcaa gtttctcaag gaacagcttc tcactcagaa gatggagatg ccaaagaaaa
     2581 cagtaatact agcgcgaaaa aacaaccaat ccatgtgaac aaggttggta gaaatgatcc
     2641 ttgtccttgt ggaagcggga aaaaatataa gaactgtcac ggaaaagatg caaactaatt
     2701 tctgacagca ggattggtga gacaaattct tttttaaaga atttgtctca ccttctttct
     2761 gttatttgac gattctgtta gactagatat aataaaaaaa gtatagtgaa agaaggaaaa
     2821 aaacatggaa atatccgaaa ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag
     2881 cttcaggagg tctctttgac ttagaccagc tggaagaaga cattgcggaa gcagaagcaa
     2941 aaatggctga acctggtttt tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa
     3001 acaatgcaaa taaagaaact tatgatcaat tccattctct tgctgaagaa ctggaagaaa
     3061 tcgaattgat ggtggagatg ttgcaggaag aacctgatgc agtaatgcaa gaagaagcag
     3121 aagcacgtat caatgcgcta gatgaaaaaa tgaatacgta tgaattatct atgttgttag
     3181 atggtcctta tgatcgaaac aacgccatcg tcgaattgca tccaggggct ggcggaacgg
     3241 agtcccaaga ctggggcagc atgcttttac gcatgtatac acgctgggca gagcagcatg
     3301 ggtatcaagt agagacacta gattatcaag caggcgatga agcaggaatc aagagtgtga
     3361 ccctactgat caaaggacac aacgcatttg gctatctgaa atcagaaaaa ggtgttcatc
     3421 gccttgtacg tatctcgcct tttgactcag caaaacgtcg tcatacttct ttttgttcgg
     3481 tagatgtgat gccagagctt gatgaaacga tcgatataga aatcaatcca gatgatttga
     3541 agatcgacac atatcgggcg agtggtgcag gtggtcagca catcaataag acagaatccg
     3601 ctgtacgaat cactcacttg cctacaggaa cagtagtagc gagtcaggca caacgttcac
     3661 aattgaaaaa ccgggaacaa gcgatgggga tgctgaaagc taagctttac caattagaag
     3721 tggaaaagaa agaacaagaa gcagctgcac tgagaggaga acaaaaagaa atcggctggg
     3781 gatcacagat ccgttcttat gttttccatc catattctat ggtaaaagac catcggacaa
     3841 acgtagagac aggaaatgtc caagctgtta tggacggaga tcttgattta tttattgacg
     3901 gctatttgaa aatgcatctg caataagagc cctttgttaa attgaaataa aattgtaaga
     3961 ttttgcaagc aagttgaaac attgccatgc tataatagct aaagactgaa aaagatatgg
     4021 agagaacgcc atgattgaaa tgaaggatgt aatgaagaag tactcaaacg gtaccactgc
     4081 tatccgcaat ctctcgattg tcatcgaaca aggagagttt gtgtatgttg ttggtccgtc
     4141 tggagctggt aaatctacat tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg
     4201 aactttgaac gtcgcaggtc atgatctaat agccatcaaa aaccgcgaag ttccttatct
     4261 acgcagagaa atcggcgtgg tgttccaaga ctataaatta ttaccaagaa aaacagtata
     4321 tgaaaatgtt gcgtacgcca tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg
     4381 agtaatggaa gtgctggatc ttgttggttt gaaacacaaa gtccgagtct ttcctagcga
     4441 attatctggt ggggaacaac aacgtgtttc tattgcccga gcaatcgtaa atacacctaa
     4501 agtacttatc gcggatgaac cgacagggaa tctagatccg gaaaactcat gggaaatcat
     4561 gaaactatta gatcggatca atgcacaagg aacaactgtc gtgatggcta cacataacag
     4621 tacgatcgta aatacgattc gacaccgagt aattgctatc gaaaacggcc gcattattcg
     4681 agaccaagcg gaaggggaat atggatacga tgattagaac ctttttccgc catttattag
     4741 aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt agcttcggtc agtgcggtaa
     4801 cgatcacgtt gactttagtt ggtgtgttca tggcggtcat catgaacgca accaaactag
     4861 cacaagatat agaaggaaac gtggatgtat ctgtgttcgt agacattggt acaaaagacg
     4921 aagagatgaa aaaattagaa aaagaattaa atgacttaga tcatgtcaaa aaagtcgaat
     4981 tttcaagcaa agaacaggaa ttgaagaaaa tccaaaacga aatgggcgat tcttggaaat
     5041 tgtttgaagg ggataataac ccgttatatg atgtatacgt agtaagtgct accgatcctt
     5101 cttatacaaa acaagtagca gaaaaagcgg cagatttgaa gaatgtttcc cgttctgatt
     5161 acggtggggc ttcctcagat cggattttcc aaattgctgg tgcagttcga acatggggat
     5221 tagttgcggc ggcattgctg ctgtttgtag caatgtttct catttctaac acgattcgga
     5281 tcacgatttt atctagacag cgtgaaattc aaatcatgcg tttagtaggg gcaaaaaatg
     5341 gatatatccg ctggccattt ttccttgaag gtggttggat cggcttaatc ggtgccattc
     5401 ttccgatact gatcattaca tttggttacg catggtttta ccaattgatc aatccgtcat
     5461 tattacgatc tcattattca ttgatccatc cagaaaatat cgtttggaaa atcaatcttt
     5521 tgatggtcgg tattggtgtg atcattggtt cactcggctc gatcatctct atgagacgtt
     5581 tcttgaaatt ctagtgtcac aagataaata taatcagaaa gttggacgat ttcgttccag
     5641 ctttcttttt ttatcagcta ggtggagtaa atggggcggc ttttttctct ttactttaaa
     5701 aaacaacttg ggtattctta agttggtaga aaaagaggtg aagccgtgaa gattatcgtt
     5761 gacaattttt ttacgatcct tttgattatg aatattttgc tttcctttat tatcgttttt
     5821 cgagaaagaa aggaaactgc gcaaacatgg gcatggctgc tcgttttgat gttcattcca
     5881 gttgtaggat ttattttata tattttttta ggaagaggga tttccaaaga gaaaatcttt
     5941 gatttgaaga tccaagggaa aatcggtaag aatctagaaa tcgaagaaga taaacaagca
     6001 ttgatgcgag ggctttatcc tcatccgcct actggacaag tagatgtgaa acaattgatc
     6061 tatatgctta ctgtatttga aagtacgctt tatacgacta aaaatgaaat cactttattt
     6121 acagatggtc gtgaaaaatt cgatgcgctg atcgaggata tcaaacaggc agaagaccat
     6181 atccatttcc aatattatat ttaccgcagt gatacattag gtgaagaagt tcgagaggca
     6241 ttgacagagg cagcaagacg cggagtaaaa gtcagagtcc ttcttgatgc atggggttcc
     6301 acacaagttt cttctagttt ttttcagaat ttgaaaaaag caggtggaga agtcgcattt
     6361 ttctttcctc tattcgttcc gtatatcaat cctcgaatca attatcgcaa tcatcgtaaa
     6421 atcgtagtga ttgatgggaa aatcggatat acaggtgggt tcaatgttgg aaacgaatat
     6481 ctaggattgg taaagaaatt tggttattgg cgagacaatc atctgcgtat ctatggagaa
     6541 gcggtttaca ccttgcaaaa ccgttttttg atggactgga attctcagca tacaaaagaa
     6601 gtaggatatt caccggaatt tttcccgacc attcattcta caggtgatat ggctgttcaa
     6661 atcgtgacaa gcggaccaga taccgaacat gagcaaatca aaatgacata tttgaaaatg
     6721 atttcgttag ccaaacggga gatattgatc caaacgccgt attacatacc ggatggatcg
     6781 attcatgagg cattgaagtt ggctcttctc tcaggcgtaa aagtccatat ccaaatacct
     6841 aacaaaccag atcatcttct cgtttactgg gcaacgtatt ctttcgctgc agagctgatc
     6901 gagtatgggg caagaatcga aacatatgaa aatggattta tccatgcaaa gacgatgatt
     6961 atcgatggcg gcatcgtttc cgttggttca gctaatatcg acgttcgctc atttagattg
     7021 gattttgaag tcaatacact gatctatgat gaacgaatgg cttctcgcgt aagaaaagca
     7081 ttctttgaag attcaaagat ttcgacgcat ctcacaaaag aagtgtatga aaaccgaggg
     7141 atgatgatca agatgaaaga aggattagca agactgattt cgccgctcct ttaaaataca
     7201 aaaaggacag gagagccatc aatagatgga aatctcgcgt ccttttttct cggggccatt
     7261 tctgagagta cgtcagaaat ggagaaagcg tctcgcatga gcgaaattaa ctttggggta
     7321 tctcttgttt tttggggaaa gcaagaaata agctaatata tgctattaaa agagacacat
     7381 ttcaaagcca gtatagcata gaaactttta atgagatagc tttttttgca attatgagaa
     7441 tttattttgt tatttaatga ttttgattcg ttttcatcaa gtaatgaaaa tgatagaata
     7501 aacatacttt aggattaaag gagatttcta atgaaaaaac taaaattatt cttaccaata
     7561 ataggattag ctattctttt atcaggctgt tcaaagtgga ttgatcgagg agaatccatt
     7621 actgcagttg gctcttctgc cttacagcct ttagtagaaa cggttgctga aacctaccag
     7681 gcagaaaatc ctggaaaatt catcaatgta caagggggcg gaagcggaac tgggttgtct
     7741 caagtacagt caggagcagt cgatatcgga aattctgatt tgttcgcgga agaaaaatca
     7801 gggattgatg cttcggcatt agtcgatcat cgagtagcag ttgttggaat cactcctatt
     7861 gtgaacaagg atattggcgt aaaagatatc agtatggaaa acctgaaaaa attatttacg
     7921 ggcgagatca aaaactggag tgaagtcggt gggaaagatc agccagtagt tatattgaac
     7981 cgtgcatcag gaagcgggac ccgtagtacg tttgaaaaat gggtattgga cggagaaaca
     8041 gctgttcagg cacaagaaca agattcaagc ggaatggtgc gccaaatcgt tgcggacaca
     8101 cccggagcaa tcagctatgt tgctttttct tacgtttccg atgatgtgga tacgttgaac
     8161 gttaacggtg tggaaccaac agatgaaaat gtgacaacga acaaatggac gatttgggca
     8221 tatgagcata tgtacacaag agaccatcca aaaaaactga caaaagaatt tttagactat
     8281 atcttatctg atgaagtaca agataatatt gtcggagagc taggctatat ccctgtttca
     8341 catatgaaag ttgaacgtga ttggcaagga aacattgtta aagagtaatc tttacacaaa
     8401 atttacaaac ttagctaaac ttttacatgt tattaaaacc aaattgatgt tcaatttgta
     8461 cactgagtga gtatgttaaa cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa
     8521 gtattattaa caaaatcaaa acgttcaaaa atggaacagc gcggaaaatt cattagtttt
     8581 ttgtgtatcg cgctgatcgt gttggttgta ttatctattt tttattttgt cgctagtaaa
     8641 ggattggcca ccttttttac tgataagatc aatgtatttg attttctgtt tggtacagat
     8701 tggaatccaa gccaaatagg agcaaatggg aaacccatgg taggggcatt gcctatgatc
     8761 acaggttcat ttattgtgac ctttttatct gctattgttg ccacaccatt tgctattgga
     8821 gcagcagtgt tcatgacgga gatttctcct aaaaacggaa cgaagatctt gcaaccggtc
     8881 atcgagctac ttgtaggtat tccgtctgtc gtttacggat ttatcggttt atctgttatc
     8941 gtgccttttg tcagaagtat ctttggcggg acagggttcg gtatcttagc tggtacattt
     9001 gtgttattcg ttatgatctt accgactgtc acaacgatga ctgtggatgc attaaaggca
     9061 gtgccgcgac actatcggga agcttcatta gcgctaggtg ccacaagatg gcaaacaatc
     9121 tacaaagtgg tgctgcgagc agcagtgccc ggaattttaa cagcagtcgt ttttggtatg
     9181 gctcgtgcgt ttggcgaagc cttagctatc caaatggtta ttggtaatgc ggcattaatg
     9241 ccaacaggac ttactactcc tgcttctaca ttgacgtcca tcttgacaat gggaatcgga
     9301 aatacaatca tgggaacagt tgaaaataat gtgttatggt cattagcgct gatcttgcta
     9361 ttgatgtcat tattctttaa tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa
     9421 taatgaacgc aaaacgctca gataaaattg ctacaggtgt tctatatgtg gtttcaggga
     9481 tcatcgtatt gatcttagca gctttattac tttatatctt agttcgagga ataccgcaca
     9541 tttcatggtc attcttgact gaaccttcca aagcctacca agaaggcggt gggatcggga
     9601 ttcaattatt taactcgatt tatttgcttt tgattaccat gatcatcagt tttcctatct
     9661 ctttaggtgc gggtatctac ctttcggaat atgccggaaa aaactggctg acagacgtga
     9721 tccgtacgtc tatcgaaatc ttgagctctt tgccttctgt tgttgtcggt ttattcggtt
     9781 tcttggtttt cgttattcaa ttccagtatg gtttctcaat catttctggt gcattagctt
     9841 tgaccgtatt caacttgcct cttttgactc gaacggtcga ggaatcttta caagcggtcc
     9901 attacacaca acgagaagct ggattggcgt taggtttgtc acgctgggaa acagtgataa
     9961 aagtggttgt tccagaagcg acaccaggaa ttttgactgg tgtgatccta agttcaggaa
    10021 gaatttttgg tgaagctgcc gcattgattt atacagctgg tcaaagcgcg ccagcattag
    10081 attttacgaa ctggaatcca ttaagtgttt ctagtccgat cagcattttc cgccaagctg
    10141 aaacactggc tgtccatatt tggaaaatca acacagaagg tacgatgcct gatggtgttt
    10201 ctgtttcggc tggcgcttct gccgtgttga tcattgctgt tttactgttc aacttcggcg
    10261 ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc agcctaagga gaagaagtaa
    10321 aatgaaaaaa tataattgga atgaaacaaa tgtcattacc atgccaaaag ataaggaagt
    10381 tgcattatat acgaatgatc tgcatgtttg gtatggcgac aatgaagcaa tcaagggagt
    10441 ggatctagaa ttcgaaaaaa ataagatcac tgcattgatt ggtccctctg gctgcggaaa
    10501 atccacgtat cttcgttctt tgaatcgtat gaatgatggg attgctaata cgaaaattac
    10561 tggaaagatt atgtataaag acgtggatat caatgcgcct caagtggatg tttatgaaat
    10621 gcgtaaacgg atcggcatgg tgttccaacg ccccaaccct tttagcaaat cgatttatga
    10681 caatataact tttgctttga aacagcatgg tgaaaaaaac aaaaagaaat tagatgaaat
    10741 cgttgaaaca agcttgaaac aagcagcttt gtgggatcaa gtaaaagata gcttggataa
    10801 aagtgccttg gctttatctg gcggacaaca gcaacgtttg tgtatcgctc gtgcaatcgc
    10861 gatgaaaccc gatatcttgc tgttagacga gccggcaagt gccttggatc ctatttcgac
    10921 aggaacggtt gaagaaacac ttgtaaactt gaaagaagac tacacgatca ttatcgtgac
    10981 gcataatatg caacaagcag ctcgtatcag cgactatact gcctttttct atctaggaaa
    11041 agtaatggaa tatgacaaaa caaacaagat atttaccaga ccaaaaattc aggcaaccga
    11101 agattatgtg tccggtcatt ttggttaaga cggaggcctc aacatgacaa aagaaattat
    11161 ctcatccaaa gatcttcatc tgtattatgg aaagaaagaa gcactaaagg gaattgatct
    11221 aagtttcaat caaggtgaaa tcacagcgat gatcggtcct tctggttgcg ggaaatctac
    11281 ctatctacgt tcgttgaatc gaatgaacga tctgattcca aatgtaacga tcaccggaag
    11341 tgttttatat aaagacaaag acatctatgg tccgaaaaca gatgttgttg aactaagaaa
    11401 agaaatcggc atggtgttcc aacagccaaa tccatttcct ttttctattt atgaaaatgt
    11461 catctatgga ttgaagttaa aaggtgaaaa agataaaaaa gtccttgatc aagtagtgga
    11521 agaaagtctg aaggcagcgt ccgtttggga ggatgtgaaa gataaactgc ataaaagtgc
    11581 cttgtcttta tccggtgggc agcagcagcg tgtatgtatc gcacgtgttt tggcagtgaa
    11641 tcccgaaatc atcttattgg acgaaccaac aagtgcactt gaccctgttt ctactgggaa
    11701 aatcgaaagt atgctgttag aattaagaga gcggtatacg atgatcatcg tgacccataa
    11761 tatgtctcaa gcttcacgta tttcagataa gacggcattc tttttagatg gtcacttgat
    11821 cgaatacaac gatacgaaga agatattcat gaatccagaa aaacaagaga cagaagatta
    11881 tatttcaggt cgatttggat aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga
    11941 attgctgaat ctccataatc agttctacga aatggggatg atggtgagca gcgctgtgca
    12001 caagtcagtt cgtgcataca tcaaacacga taaaatctta gctcaagaag ttatcgaaaa
    12061 cgatataaat atcaataata tggaaacgcg tttggagaag aaaagttttg aaatgattgc
    12121 attacaacag ccagttacaa ctgacttgcg tatgattatc acggtcatga aagctagctc
    12181 tgatttagaa cgtatggccg atcatgcggt gtccgttgct aaatccacaa tccgcgtaaa
    12241 aggacagacg agaataccgg aaatcgaaaa agagatctcc gatatgtccg attacgtgaa
    12301 aaaaatggtg gacaacgtcc tagttgcgta tgtcaaaaca gacgaaaaag atgcgcgtac
    12361 aatcgccaat atggatcaaa gagtcaatga ctatttcaat cgaatctata atgcaaccat
    12421 caaaaacatg caagaaaatc ctgaaacagt catcagtggc acagactatt tgaatgttgc
    12481 cagctatcta gaacgaatcg gagactatgt gacaaacatc tgtgaatgga tcgtctactt
    12541 ggctacaggg aaaatcactg aattaaatac gaaccacaat gagattttct aaactgaagt
    12601 tgtgattctg ctgtttattt aggaaaagag gttgtgaaat cagcgttttg tcctgagtat
    12661 tagagaaaca aacggtaaaa tagttcctca tattttgccg ttttttgagc taataccgaa
    12721 ggtcagctga ttgaacaccg tttattctgg aaaagggttt gtgacaaaac atgtcacaaa
    12781 cccttttcat tattccaata gaaaggataa agaatgcaaa aaaagattag catcatcatc
    12841 actctatttt ttatttgtat acttgcttca tggtttattc agcgtcaagc tgctctatat
    12901 gacgtacctg tcatcgagat cgaaagagtg agaatagaag aggatcaaca attgattaca
    12961 ggaagattga tgaatacgga ccaaaaagga gaagtgattt ctttaacggc tccttatcaa
    13021 aaaaatcaaa tcgaaacagc aaatataaca acaagacaaa tctatcttgt ccaaatgcat
    13081 ggagatgaat ggcaactagt agcaaaaaaa aatgatggct ggcttttttt ctttacaagt
    13141 gtcttcattg gcaccatgtt gcttattggt ggaaaacagg gcgtatttgc tttaggaagt
    13201 ctcatcatca acatcgtttt acttttactt ttgttgctcc tctttaccaa aacagaaggt
    13261 atttctctct taaatatagc aatcttattt gtcgttatag ggataatcgt tagtatattg
    13321 gcgttagacg gatggaatag aagcagtata atcaaaattt cggctgcagt tacaagtgtt
    13381 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagatca aggactgcgc
    13441 tatgaagaac tgaattatct gacacgaccg tatcgttctg tttttctttc cagcgtgctg
    13501 attgggacag ctggagggac actcgatacc gtgatcacag tcattgcgac attagaagaa
    13561 ttgaccaagc aacaaccgaa aatatcagcg aaacagttat ggctttcagg aaggcaagtg
    13621 ggccgagatg tgattggttc gatggctaat atccttttat tcgtctatat cagcggagcg
    13681 attccatcgc ttctgttata tttaggaaat caatggtctt tcaaagaaac ggtagaacga
    13741 catttatcat tagaactttt acgcgtcatt gctggcagct taggaatagt attatcaatc
    13801 cccatcgcgg tcctcttttt tctcgctgta aggaggttaa aaaaatgaat gtacttatta
    13861 tacttgttgg gatttttgca atcagtgtgc tatttgtcgg tggtacgcaa ggtatgtata
    13921 ttcttttagg attattcatt aatttaggaa tctttttcct attattattc ggctatcatc
    13981 agaagtggcc gatattggta ttgtcgatta tagggttcct attgatcgct gtggttatct
    14041 tattttttat caatggttat aatctgaaaa tgcgagccgc ttttgccagt atactgatct
    14101 ttttgttttg tttcctacta ttgattccta tcactgattt tttagcgatc caaggcttta
    14161 cttcgataga actagaagaa ctatctggct tagataagac gttagcaatt gattttagac
    14221 tacttacgag gtcgttgctt ctgatcagtc ttagcggagc tgttttagat gcgagtgtag
    14281 cgatttctag tggaacattc gaagtatatc aggcaaatcc gcaaatgtct tttaaccaat
    14341 tgcgccatgc tagctttgcc attgctaaaa aaatattggc atcaacagtg atgacgcttc
    14401 tttttgcgtt tatggggagt tctttagcgt tgattctttg gtttattgac ttagacattt
    14461 cttttcagca aataatcaat gaaaaatcat ttgtcttgga atatacgatg gctattctta
    14521 ccacactggc cgcactactt gtcctgccct taacttgcgt gactacagca tatctcttta
    14581 caaaaaagaa agaacatctc aagatggagt aaaagttaca acttaagtcc tatgacattt
    14641 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
    14701 aggcaatttc catgaaagaa agagaacgta ttttagactt agtgaaaaaa ggaatccttt
    14761 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaaacaaa
    14821 tcaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
    14881 tgatcgatga acttgaaaac ggtgaagaaa aaatttctga gccagttgat ccaaaagaaa
    14941 aagaaagaca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
    15001 ataaaacttc ggcgcgtcta gacgaagtaa atgctcaatt cgctgacaat aaggcagcac
    15061 gtaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
    15121 cagaagaaga attagcacag cgtcaaacgt tagaagcaga aatcaaagag ttagaagcaa
    15181 ccggcgatac actattagaa gaacaattga agctggaagc agaattaaaa gatatccgca
    15241 agaatcaatg gtctgagaaa aaagaaacat ttacggataa gtttgaattg ccagatgatt
    15301 ggaaagatca agcgacagat acgctgaatc aagtaggcga aaaaatgtca gaagctggat
    15361 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtaaatgata
    15421 acatggaatg gaaagatgtc agcttacgcg tccctggaat tgcgacaacg aaatttgaac
    15481 acgaatttta ttatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
    15541 acgtgacgtt aaaaacgtgg gacagtgatg atgtgaaagt ggaagccaag atcaaacttt
    15601 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag atcgaggtca
    15661 atgaagatca tatctccttc caaattccaa ataagcgtgt gcgtgcagac cttgttttct
    15721 acttgcctaa acgagtatac gaccatgcag ctatcaaact tctaaatggc aacatcatga
    15781 ttgagacatt ggaagctaaa gatatttata caaaatctac gaatgggaac atcatcgtga
    15841 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
    15901 acgggaacat cttagattct attatagaaa cagtaaacgg tacagtgaca tttggtgcca
    15961 caccagaaaa cctttctgtg tctttagtaa atggtgatgt acgtcttacg attaaagaag
    16021 ataatttgaa aaaagtagaa gcaagttcag tgaacgggaa tgtcaaagtt gctttaccag
    16081 atagcatcgg tttggaagga cacgctaaaa caagtcttgg aagcattaac agccgtctgt
    16141 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
    16201 gtgtttcaga tgatgagatc gcacaagtgc agctttcaac gacaacagga agtatctatt
    16261 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
    16321 gcgatttttt aaaatagctt cccctatttt aaaaaaatgt gccttattgc cgataggcta
    16381 tctcttgaac accgattaag aagaaggttg tcacaacgat tttcttcatt taaaatagta
    16441 gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ccaataaaaa tgttgttttg
    16501 acaggtactt tagcagggat tggggaatat atcgggattg atcccactgt tatccgtgtg
    16561 ctttatgtat tcttaagctt agttgctttt ggaagtccaa tcattttata cattttatta
    16621 gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa caatgcatat
    16681 tatgaaaaaa acaattatcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16741 gatgacgaat ggagtgactt ttaatgtcgt actttcagcg tattttggtt aatacgttaa
    16801 cattcatatc gttggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    16861 ccgtccttgc cagcttcgta ttatctgtgt tgaacatgtt tgtcaaaccg attttgacga
    16921 tcctgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    16981 cgattcttaa aatgacttcc ttttttgtag gagcagaaca ttttagtttt tcttcatttg
    17041 gtgcagcgtt attcgtagct atcttgatgt cacttgtcaa tgtaattgtt agtgaacata
    17101 acagcagaaa ataaaaaaag ctgtgccgag atctcggcgc agcttttttg tttagaataa
    17161 agaatgcgtt ttctattaca ttggtaatta aataaaaacg ttttttttaa ttggtagtac
    17221 ttttggctag acaatgataa aatgatagag aacactctaa tagaaatgag gtagcatgaa
    17281 tgtcagaagt tgtaaaagtt agccaattag taaaaaaatt atctttggaa atcgtcacag
    17341 gcgacgagaa aagtttagac cgcgtcatcg tgactgggga catctcacgc ccaggactag
    17401 agttgaccgg ttattttaat tattattccc ataatcgttt acagctattt ggcagtaaag
    17461 aaatcagttt tgcggaacgg atgatcccag aagagcgttt gatcattatg agaagattat
    17521 gtagtgaaga tatgcccgca tttattattt caagaggatt gccggctccg gaagaaatga
    17581 tccaagcagc caatgaaaat gggatagctg tgctgcgttc gcctatctca acgtcccggc
    17641 tgctaggtga attatctagt tatttagatg gacgcttagc ccctcgaaca agtgtccacg
    17701 gtgtgttagt cgatgtctac ggattaggtg tactaattca aggggacagt gggataggga
    17761 aaagtgaaac ggcgttggaa ctgattaagc gcggacatcg attgatcgca gacgatcgag
    17821 tagatgtcta tcagcaagat gaactgacgg taatcggaga acctcctaaa atcctggaac
    17881 atctgattga gattcgaggg atcggtatca tagatgtaat gaatcttttc ggtgcgagtg
    17941 cggtacgagg ctctatgcaa gtccagttag ctgtttatct agaagcatgg gcgaaagata
    18001 agaaatacga ccgtctgggc tctgaagaca cgaccgttga aatcgcggaa gtgggcattc
    18061 cacaaataaa aatccctgtc aaaacaggac gaaacgttgc aatcatcatt gaagtagccg
    18121 caatgaattt ccgcgcacgt acaatgggct ttgacgcaac gaaaacattt gaagaaagat
    18181 taagtcgctt gattgaagaa aactcaggca atgactagca aggagtgaca atatgttagc
    18241 acaaatcaat cgagttgctt ttgagcttgt aggaattcct atctactggt atgcattgat
    18301 tatcgtatca ggaatcatta ttgccatgtg gctcagcagc agagaagctg ttcgggtagg
    18361 attaaaagca gatgacgtga cagattttat gttgtgggga ttgccgattt ccattattgg
    18421 tgcccgcttg tattatgttt tattcgatct tccccaatat atagctgatc caatccagat
    18481 attcaatatc cgttcaggag gattagcgat ttacggggga ttgattgcag gtgcaatcac
    18541 actgtacttt tttaccaaat atcattttat ttctttgtgg acctttttag atatcgctgc
    18601 accaagcgta ttattggcgc aggcaatcgg acgatgggga aatttcatga accacgaagc
    18661 gtatggtcca gacacgacac gaagtttctt agaaagtctg catttaccgc aattcatcat
    18721 tgataacatg tatatcgaag gaatctatcg acaaccaacc tttctttatg aatcactttg
    18781 gagtttgatt ggatttattc tgcttttggt tttgcggaag aaaccacatc ttttgaaaaa
    18841 aggagaagtc cttttatgtt atctaatgtg gtatagtttc ggacgtttct ttatcgaagg
    18901 gttacgcaca gacagccttt actttttagg tgtgatccgc atttctcaat tattgtctgc
    18961 tgttttattt gtcggcacat tgattctgtt tatttggcgt agaaaaaaag gaagtttacc
    19021 ggattatgac cgctcattag gaaaaaatca acaaattatt tgattttcag agcgttaaaa
    19081 atgaaaaaaa tttcaggagt ggtaaaatga attatagagt agcggtttta ggaccaggct
    19141 cttgggggac tgctttagca caagtcctcg ctgaaaacgg acatgacgtt cggatttggg
    19201 gacatcgtgc ttcacaagta gaagaaatca acacccaaca caccaatcaa cgttatttac
    19261 cagatcatca tctacccgat tccatccgag cattcaccga tatggaagat gcagtaaaag
    19321 atgcagatgc ggtactattc gttatcccga cgaaagcgat acgttctgtt gcaaaagaac
    19381 tagttgccaa attgaatacg aagccagtga tcatccacgc aagtaaggga ttagaacagg
    19441 gaacgcataa aagaatctct gaaatcttgt tagaggaaat cccgacagaa aaaagaaaag
    19501 ctgttgctgt tctttctggg ccaagccatg cggaagaagt agcaaagcat gacattacga
    19561 cgatcacagc ggcaagcaca aatgtaaaag cagcaaaata tgtacaagag ctgttcatga
    19621 accattattt tcgaatctat accaaccctg atgtgattgg tgtagagatg ggagcagctt
    19681 tgaaaaatat tattgccatt ggagctggtg ctattcacgg cttagggttt ggcgataatg
    19741 caaaagcggc aatcatgacg cgcggattag ctgagatcag ccgtttaggt gtagcaatgg
    19801 gtgccaaccc gctgaccttt attggcttaa gcggagtagg tgatctagtc gttacttgca
    19861 caagtgttca ttccagaaac tggcgcgcag gcaacttgtt aggacaaggt cagcctctag
    19921 aagaggtgct agccaatatg ggaatggttg ttgaaggtgt gaatactact caagcagcga
    19981 tggaactagc ccaaatgatg gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc
    20041 tgtatgaaca aaaagaaatc aaaacagccg caaaagaaat catgttacgt gatgggaaaa
    20101 aagaaaatga attctcatta acaatttttt gaaggagcga atgtatatga aagtaaaaaa
    20161 agcagttatt ccagcagcag gattaggaac aagattttta ccagcaacaa aggcgatggc
    20221 aaaggaaatg ttgccaatcg tggacaaacc aacgatccaa ttcatcgttg aagaagcgtt
    20281 gaaatcagga atcgaagaca tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga
    20341 tcactttgat tcaaatatcg aattagaaac aaacctaagt gaaaaaggaa aaaccgaatt
    20401 attgaaatta gtagaagaaa cgacagatgt caatttgcat ttcatccgtc aatctcatcc
    20461 aaaggggtta ggacacgccg ttcttcaagc aagagccttt attggaaatg aaccgtttgt
    20521 cgttatgcta ggcgatgata tcatggaaga cgaagtacca ttgacacagc aattgatcga
    20581 tgactatgag cgtacacacg cttctacgat tgccgttatg caagttcctc acgatgaaac
    20641 ttcaaaatac ggaatcatca acccagggga agtactggaa gatgggttat acaatgtcaa
    20701 aaactttgtt gaaaaaccag atccaagcaa agcaccaagt gatttagcta ttattgggcg
    20761 ttacttattg actccagaaa tctttgatgt attatcagaa caacaaccag gcgctggaaa
    20821 tgagatccaa ctaactgacg caatcgatac gttgaataaa acacaacgcg tatttgcccg
    20881 taaattcaca ggtaaacgtt atgacgttgg tgacaaattt ggttttatga aaacaagtat
    20941 tgaatatggt ctgacacatc cagaagtagg ggcacctttg cgtgaataca tcatgtcttt
    21001 aggtaaaaca ttagaagcag aaggaagcaa gatcaatcat aatccaaaag gaataacaga
    21061 aatcaaagaa gaaaagaaaa aataatcttt ttttctaacg agcttcagct gctaattgaa
    21121 taaaaagtag ttgaagctcg tttttatttg tttaaaaaac acgaaaatgc tatccttata
    21181 gaaaagagag acaagaaggg agtttgatca tgtcaggagg agaaattgca ggattgatcg
    21241 cagcaatcgc ctttgcgata cttgtcgttt tcatcgttcg cgtattgttg caagtttcca
    21301 aaactttaga aaaagtagac cagactgtcg cagaagcaaa tacgacaatt gagatcgtca
    21361 cacaagacgt agatctttta tctagacaag tagaaggctt gctagtcaaa agtaatgagc
    21421 tgctcttgga tatcaatgga aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg
    21481 atttaagtga aagtgtatcg gaacttaatc attctagtaa aaacatcgtg acgaaagtcg
    21541 gcggaatcgg aaaaacgaca gctaaagcga ctgttgccgg aaaagtcggt ggaacagcga
    21601 tgaaattttt aaaaacaaaa aacatactga aacgacagga ggaaaataag aatggctaaa
    21661 aaaggtggat ttttattagg tgcagtagtt ggaggtacag ccgcagcaat cgccgcatta
    21721 ttgttagcac ccaaatctgg taaagaactt cgggatgaat tggctgctca agcagatgat
    21781 ttgatggaca tggcttcaga ttacacagat atggcaaaag aaaaatcatc tgagttaaca
    21841 ggtattgcaa aagaaaaagc gcaagatctt tctcaacaag caggggatct agcaggcaat
    21901 gttaagcaga aggtaagtga atcagcagag gaagcaggaa gcgaatcaga tgatatcttg
    21961 ttaaacctca aagaacaatc agctgattta tctaatcgtt tcaaaaaaac agcagaagat
    22021 ctgaaaaagc cagcagacga attaggtagt attgctaaag atacttcaga agatatcatg
    22081 atcgatgtaa aagatgctgc agataaagca ggagagacgg tcaatgaagg aatgtcagaa
    22141 gcaaaaccag ttgcagatga cacaaaagaa atgttgaaaa tggcaaaaga agatgcaaaa
    22201 gaagctaaaa aagaaatgaa agatacaatg acagattcga ctaccaatca ctaagattac
    22261 tcatttctag aataaaaaag gtgagagcgg accagctctc acctttttta ttttcattac
    22321 tctaaaacaa gtaattcttt tggtgtagtc gtgaatggca cacaaccatc acttgtcaca
    22381 tacacacaat cttcgatacg tacccctact ttttcaggaa tatagattcc tggttcaaga
    22441 gagaagcaca tcccttcttc aatcactaag tcgtttcctt ctacaagaga agggaattcg
    22501 tggacggttg ttccgattcc gtgacctaaa cggtgattga aatattcgcc gtagccatag
    22561 ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc tgggcgaact
    22621 gcctcttgtg cggcaagctg tgcttcaaga gtgatgttat agattttttc ttggaattca
    22681 gttggttttt gataagcaac tgtacgagta gcatcactgc aatagccatt ccaaataacg
    22741 ccaagatcga ataacactag ttcatttggt gaaaccttcg tgcttcctgg tgtaccatga
    22801 gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg tgaaacacct
    22861 tgttttttta attggtattc gatttctgcg ataattgctt gttcagttac gccttctttt
    22921 actgttttga agccaatttc aaaggcaaca tcggcccatt taccagcttc gatcagtttg
    22981 tctttttctt ctaccgtttt gatcagttgc agacgctgga taatagaagt tagatcggtg
    23041 taagaatctg tacctgtcaa gatatcagag atagcatcga agcgatcgac agtcagagca
    23101 ttcttttcaa tacctacatt accaggttgc ccatagcgtg cgcgaagctc ttttccgatg
    23161 atctcccaag gattttcgct gtctaagtag ccacgtacat cgtacgccca gctgctgttt
    23221 tgagcatctt caacttccag agcaggagtg aaaaggaaag gatcttgttc cagagggata
    23281 aaaagagcta gtacacgctc gtgaggatca ctttcatatc cggaaaagta agcgatgtgg
    23341 ccagggtcgc tgatataagc caattctaat tggtgttgtg ccatccattg acgtagttca
    23401 ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt attcaataat
    23461 tgtaccactg attgattaaa agaccaacgt tcttaaaact gaaaaaagta aaaattttca
    23521 tgaaaacaga gataaaacgg ttgcaattgt tgagattatt tgatatccta ttttagaaat
    23581 gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa tggaaaagca
    23641 aacaatcact atctatgatg tagcgagaga ggcaaat
//
LOCUS       DACDYM010000041        23573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDYM010000041 DACDYM010000000
VERSION     DACDYM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.041"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IWM59_001605"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IWM59_001605"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9979643.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IWM59_001606"
     CDS             complement(1174..1869)
                     /locus_tag="IWM59_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9979644.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IWM59_001607"
     CDS             complement(1882..2598)
                     /locus_tag="IWM59_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9979645.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IWM59_001608"
     CDS             2928..3533
                     /locus_tag="IWM59_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9979646.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IWM59_001609"
     CDS             3858..4181
                     /locus_tag="IWM59_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9979647.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IWM59_001610"
     CDS             4363..5733
                     /locus_tag="IWM59_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9979648.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IWM59_001611"
     CDS             complement(5863..7164)
                     /locus_tag="IWM59_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9979649.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="IWM59_001612"
     CDS             complement(7394..9385)
                     /locus_tag="IWM59_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9979650.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="IWM59_001613"
     CDS             complement(9529..9795)
                     /locus_tag="IWM59_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9979651.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IWM59_001614"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IWM59_001614"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9979652.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IWM59_001615"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IWM59_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9979653.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="IWM59_001616"
     CDS             13139..13702
                     /locus_tag="IWM59_001616"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9979654.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="IWM59_001617"
     CDS             complement(13955..14476)
                     /locus_tag="IWM59_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9979655.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="IWM59_001618"
     CDS             complement(14479..15321)
                     /locus_tag="IWM59_001618"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9979656.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="IWM59_001619"
     CDS             complement(15314..16039)
                     /locus_tag="IWM59_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9979657.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="IWM59_001620"
     CDS             16215..16511
                     /locus_tag="IWM59_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979658.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17079..18671
                     /locus_tag="IWM59_001621"
     CDS             17079..18671
                     /locus_tag="IWM59_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9979659.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18684..19424
                     /locus_tag="IWM59_001622"
     CDS             18684..19424
                     /locus_tag="IWM59_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9979660.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19592..20941
                     /locus_tag="IWM59_001623"
     CDS             19592..20941
                     /locus_tag="IWM59_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9979661.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21069..21659)
                     /locus_tag="IWM59_001624"
     CDS             complement(21069..21659)
                     /locus_tag="IWM59_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9979662.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21806..23158
                     /gene="brnQ"
                     /locus_tag="IWM59_001625"
     CDS             21806..23158
                     /gene="brnQ"
                     /locus_tag="IWM59_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9979663.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6751 a         4746 c         4490 g         7586 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    16561 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    16621 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    16681 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    16741 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    16801 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    16861 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    16921 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    16981 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    17041 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    17101 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    17161 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    17221 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    17281 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    17341 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    17401 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    17461 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    17521 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
    17581 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    17641 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    17701 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    17761 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    17821 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    17881 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    17941 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    18001 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    18061 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    18121 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    18181 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    18241 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    18301 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    18361 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    18421 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    18481 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    18541 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    18601 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    18661 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    18721 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    18781 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    18841 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    18901 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    18961 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    19021 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    19081 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    19141 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    19201 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    19261 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    19321 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    19381 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
    19441 ggacaaagag ctgcggtgtt taccgcagtt cttttttatg aattactcat aaaaggtaaa
    19501 tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa actctcttaa tatggaagta
    19561 attaaacttt tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg
    19621 actgaaaaat agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt
    19681 tttaggttcc ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat
    19741 tactggatgc atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac
    19801 caataaccat tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat
    19861 cactggctgg acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat
    19921 cggaatgtat gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc
    19981 attaggtctt cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt
    20041 ttggtttgca ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat
    20101 gatcctcacc cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca
    20161 tggcggattc tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt
    20221 tgcttttgct gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa
    20281 agtgttgccg gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc
    20341 tctttttgtc attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt
    20401 tgtagaagta ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt
    20461 attaagtgca gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg
    20521 ttctttagcg caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt
    20581 gccagggaat gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta
    20641 cgtgatgcct agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt
    20701 tatctggagt atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa
    20761 agctcattca tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat
    20821 ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac
    20881 acctgtgtgg ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta
    20941 agaaagtagt tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt
    21001 gcttcttaaa tttttgtgaa atttcggctt atttcttgga gataaaagct tctgtcccag
    21061 cctcttttct atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa
    21121 aaaatccaaa taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag
    21181 acaccggcaa tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac
    21241 aaaactctca cgacatggga atctggttta tttgctttca tgatttgcct catcaagata
    21301 ccaaaagaaa aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct
    21361 tcagggttac gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa
    21421 aagatattcg accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga
    21481 tggttcaccc agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt
    21541 cggatgaccc ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaata
    21601 actctcaaaa caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg
    21661 atgattcctc ctcagatgat tcagcgcttt tattttatca cttacgttat taaataacca
    21721 tcgttttata tagggaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt
    21781 taaaaaaacc gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt
    21841 tataggctcc atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca
    21901 tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg
    21961 tatcggtctt cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata
    22021 tgaattagca aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt
    22081 agttatcgga ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt
    22141 agcaccattt atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt
    22201 ttttgtgctg gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa
    22261 gttcctgaat cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa
    22321 tccactaggt tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt
    22381 cacagggttt actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat
    22441 catcattgtc acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga
    22501 cacgatcaaa tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc
    22561 ttatttaggt gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct
    22621 ggctcagatt gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt
    22681 tatattagct tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac
    22741 agatttgttt ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg
    22801 tttagcggca aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt
    22861 catctatcca ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca
    22921 gcgtacggct gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg
    22981 gttgaacgct tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt
    23041 tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat
    23101 cggttttgtt atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata
    23161 aaagaggttg tgaaagagac gttcagcctt gagcaataaa aagtaatttt gaaaaatggc
    23221 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgttt
    23281 attgagaggt cgtgaaaaag ccgcttagtt tcaagtatta agaaatattt tgtgaaaatg
    23341 gcttctcaca tttttgcata aatttgactt aataccgaga aactggcttt tgaacaccgt
    23401 ttattgagcg tctataaact gacctgagta ttagagaaac aaaacggcaa agtagctcca
    23461 tatactttgc cgttttgttt tgaactaata ccgaagagaa gctgatcaaa caacatttct
    23521 tttataatat agattttaga catatatatg atataaaaat agttttcgca aaa
//
LOCUS       DACDYM010000042        22418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDYM010000042 DACDYM010000000
VERSION     DACDYM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.042"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            162..1025
                     /locus_tag="IWM59_001626"
     CDS             162..1025
                     /locus_tag="IWM59_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9979664.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1154..2776)
                     /locus_tag="IWM59_001627"
     CDS             complement(1154..2776)
                     /locus_tag="IWM59_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9979665.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3025..3546
                     /locus_tag="IWM59_001628"
     CDS             3025..3546
                     /locus_tag="IWM59_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979666.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3732..5462)
                     /gene="spxB"
                     /locus_tag="IWM59_001629"
     CDS             complement(3732..5462)
                     /gene="spxB"
                     /locus_tag="IWM59_001629"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9979667.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5627..6445
                     /locus_tag="IWM59_001630"
     CDS             5627..6445
                     /locus_tag="IWM59_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9979668.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6548..6745)
                     /locus_tag="IWM59_001631"
     CDS             complement(6548..6745)
                     /locus_tag="IWM59_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979669.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6914..7492
                     /locus_tag="IWM59_001632"
     CDS             6914..7492
                     /locus_tag="IWM59_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9979670.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8537..9040)
                     /locus_tag="IWM59_001633"
     CDS             complement(8537..9040)
                     /locus_tag="IWM59_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9979671.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9399..10424
                     /locus_tag="IWM59_001634"
     CDS             9399..10424
                     /locus_tag="IWM59_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9979672.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10747..11625
                     /locus_tag="IWM59_001635"
     CDS             10747..11625
                     /locus_tag="IWM59_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979673.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12101..12217
                     /locus_tag="IWM59_001636"
     CDS             12101..12217
                     /locus_tag="IWM59_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9979674.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13145..13483
                     /locus_tag="IWM59_001637"
     CDS             13145..13483
                     /locus_tag="IWM59_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979675.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13577..13807
                     /locus_tag="IWM59_001638"
     CDS             13577..13807
                     /locus_tag="IWM59_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979676.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14207..14578
                     /locus_tag="IWM59_001639"
     CDS             14207..14578
                     /locus_tag="IWM59_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9979677.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14852..15544)
                     /locus_tag="IWM59_001640"
     CDS             complement(14852..15544)
                     /locus_tag="IWM59_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9979678.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15547..16332)
                     /locus_tag="IWM59_001641"
     CDS             complement(15547..16332)
                     /locus_tag="IWM59_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9979679.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16569..17969
                     /gene="lacG"
                     /locus_tag="IWM59_001642"
     CDS             16569..17969
                     /gene="lacG"
                     /locus_tag="IWM59_001642"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9979680.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17994..18311
                     /locus_tag="IWM59_001643"
     CDS             17994..18311
                     /locus_tag="IWM59_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9979681.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18324..19997
                     /locus_tag="IWM59_001644"
     CDS             18324..19997
                     /locus_tag="IWM59_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9979682.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20011..21642
                     /locus_tag="IWM59_001645"
     CDS             20011..21642
                     /locus_tag="IWM59_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9979683.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT         7001 a         3752 c         4511 g         7154 t
ORIGIN      
        1 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcttattttg
       61 attagaactt gcgaaaataa accggttaaa gtgtgtctag cgcaagagat ttgctataat
      121 agacgataag ggtaaaatgt aacgagaagg ggttgaaaaa tgtgtatcaa attgtaacag
      181 attcttgttg tgatctgcca taccatgtat tagcaaaaga aaaagttgat tttttatcaa
      241 tgaatctcca attgaatgat caagagttag tagatgacct cggaaagaca ttcaattatg
      301 accgctacat gaatcagctg aaaaatggtg cgatgcctac gacatcacaa atcaatgttg
      361 gccgatattt agaatttttc cgttcttatg cggaaaaaca gattcctgtt ttatatgtgg
      421 ctttttccag tggattaagc ggctcttaca gtagtgcgct tcaagcggtt gaattgctga
      481 aggaagaata tgaagaccct gagatctatg tttttgatac aaaagctgca agtattgggg
      541 aagggctttt agtattggaa gctgtaagat tgaaaaaaga aggtaaaagc atcgaagaaa
      601 ccctagagtg gctggaaagt aattatttga aagtccattc atgggtaaca gtagacgatc
      661 tgaaatatct agaacgaggc ggtaggattt ccaaagcaag tgctactctt ggcagtctat
      721 tgagtgtaaa gcctattatt acggtggata aagaaggaag gctgacgaat gttgacaaag
      781 ttcgaggaag aaacaagtcg atccagacaa tcgtcaatga gacggtaaaa acaatcgttg
      841 caccacttga tcaaatcgtt tatgtggctt acgctgggga tctagagagc gcggagaagg
      901 caaaacggct tttggaagag cagataaaga tgaaagatgt gaagctttat cccttaggac
      961 caacgatcgc cagtcacaca ggctatggct gtatcgcgat tttttcgatg ggaatcagtc
     1021 gataaaagca gtaactcatt ctgtaaaaaa gcccagaaat tgattgtaac aaaaacagcg
     1081 actcttaaaa gatagatgag catcctatct cttagaggtc gctgtttttt tattttttaa
     1141 gctaaatagg ccattatgcc cataactcgt tcaattgttt ttgaatatct gcattttcca
     1201 aaaattcatc atatgtcgta tcagctcgat cgatcacacc tttatccgaa acggcaatga
     1261 tgcggtttgc caatgtttgg ataaattgat ggtcatgtga cgcaaataag attgagccag
     1321 taaatgccat caagccgtca ttcaatgctg tgattgattc taagtctaaa tggttcgttg
     1381 gatcatctaa tacaagtacg ttagctttcg aaagcatcat ttttgaaagc atgcaacgga
     1441 ctttctctcc accagataaa acattcactg gtttcaatac ttcttcacca gaaaacaaca
     1501 ttcttcctaa gaagcttctt aagaaggtgt tatcatcttc ttctttactt gcatattgac
     1561 gcaaccagtc taaaatcgtt agatcagtat cgaattcttt cgttgtatcc tttggtaaat
     1621 aagaattact tgttgtcaca ccccaacgta ctgttccagt atctggcgta atttcaccca
     1681 tgataacttt gaatagcgtt gttgttgtta tatcattttc tgcgacgaaa gcgaccttgt
     1741 cttctttgct caaattaaaa gatatatcat ctaaaatttt ctttccatca atcgttacac
     1801 ttacattttc tacctgcaat aagtcgtttc caatctcacg ttcaggtttg aatccaacga
     1861 atggataacg tctagaagaa ggttggatat cgtctaaagt aattttttca agcatttttt
     1921 tacgtgaagt tgcttgtttt gattttgatg cattcgcact aaagcgggca ataaattctt
     1981 ggagttcttt gatttgttct tcttttttgg catttgcgtt tgcttgaagt ttagccgcta
     2041 gttgactaga ttccaaccaa aaatcataat ttcctacata aagtttgatt ttaccaaaat
     2101 ctagatccgc catatgtgta catactttgt tcaaaaagtg acggtcatgg gatacaacga
     2161 tcacagtatt ttcaaaattg atcaaaaatt cttccagcca attgatcgat tggatatcca
     2221 gaccgtttgt tggttcgtct agcaaaagaa catcaggttt accaaatagt gtttgtgcta
     2281 ataacacttt taccttttgt ccaccagtta attcactcat ttgctgatgg tgcaattctt
     2341 cagggatgtt cagaccttgc agtaaaacag ctgcttctgg ttcagcttcc catccattaa
     2401 gctcagcaaa ttctccttct aattcagctg ctcgaatacc atcttcgtca gtgaaatcct
     2461 ctttcatgta aatcgcatct ttttctttca gcacttcata cagacgcttg tgtcccatga
     2521 tgactgtttc aagtacagta tagtcttcat agtcgtaatg gttctgtttc aatacggcca
     2581 tccgttcatc tggtcccata gtaacggaac ctgtggaagg ctgcagatcc cctgataaca
     2641 ctttaaggaa cgttgattta cctgcaccat ttgcaccaat caagccatag cagtttccgg
     2701 gggtaaattt aatattcaca tcatcaaata gtttgcggtc tgaaaagtgc aaactcacat
     2761 catttacagt aatcaatttt tttcctcact tttatcatta ttagttttga caagtcttat
     2821 agaattatat aggggaaagg taaaagattc aaggcaatcg aacattcttt gtgtttcacg
     2881 tgaaacagaa tgaaaaaaat aaaaataaaa gcggagcgaa aatagctact ataggattta
     2941 ttagtttgca acagcataat ttttgacgtt atttataaga gggagtacta taaaactgta
     3001 aaagaaatga aagaaggatt gaaaatggat gaaaaaagaa ccatcgactg gggctcattg
     3061 attttgggaa ttttatttgt acttgtctca ttgttatcat ttcaagaccc tgtcggaaat
     3121 ttagttgcaa ttgtagtagt ctttgcgatt tttgccttta ttaaagggat atttgaactg
     3181 tttgtacgga acagattaaa agagctaaca ggatataaag gaaagatgcc cttaataatc
     3241 gggatcatcg atattctagt tggcgtcttt ttcttattca atattggtgc aggagtcgca
     3301 gcattgcctt ttgtttttgc agtatggttt attgctgatt cagttttagc gttatttact
     3361 gctgacttgg caagaggagt aagtgaagga tattattggt ttacgatcat cgtcaatatt
     3421 ttaggaattt tattaggaat tttcttattg tttaatccaa tttcttctgc gttgacatta
     3481 agctttttag ttggattcta ttttatgtta tttggtatca cacatatcgt ctatgcattt
     3541 agataagtaa aatcaaacaa ataaaaaagc cggcttccga ttacagtttt actgttgtaa
     3601 ttggaagccg gcttttttat catggacaaa tatgcacgag gtgattttta ggtgtcgaga
     3661 caaatttatg caaaaatgtg agaagctata tacacaaatt ttatcttaat actcgaagca
     3721 tagcgcagtt gtcacaacct cttttcatat aattcattca atactggcaa atcatggact
     3781 tcatattttt ctttgaatgc ttggatttct tcttcagaat attttcttgg atctaaatgc
     3841 agatcttcta ctggcaatgg gcgcttgtta ttgatcttca catcgattac tacaggacga
     3901 tctgattttg cagctgcatc aaaggcaaat cgaagttgcg agtattctgt gactgtaaag
     3961 ctatttgccc cgagcgcttc tccaacttta ccgaaatctg catcttctaa aaatacacca
     4021 aatttctttt gttccgtatc ttcttgttct gcttcgatga aaccaaaaga atcattcgaa
     4081 aagacaacat taataattgg aagctgatat ttgacttgtg taatgatatc ttgcatgacc
     4141 atagcaaacc cgccatcacc acttaaggta aatacttgtt tttctgggta gcttagttgt
     4201 gcggcaattc ctcctggcac accattaccc atagttgcga accatccaga tgtcgtatgt
     4261 ttttgtttac cgttcatatt caataaacga atagaatgga tagtcgtgtt accgacatct
     4321 gtgacgaaaa tcgcatcgtc ttcagcaata cgattgattt ctttatagac agcctctgga
     4381 cgaatcggtt cttcctctcg atcttcaaaa gatttcaacc aatttaccca tttttcttta
     4441 ttttcttgat ttgctaggta ccagctatca gcagggcgag catcccctaa ctcgacaagt
     4501 ctttgcaaag ctgtgtttgc atcaccgagg atcgacaaag atgtttcatg gcgtcgtcca
     4561 aattttgacg catcgatatc gatttggata aattcagcag ttggattaaa aaatgcacgt
     4621 ccaaatggga agtcgctacc gacaaataag atcagatcgg cttctgccaa tgcttcattt
     4681 ccgggtttag tagcgacacg tccagcaaaa ccaagaaagt tttcataatc atcaggaata
     4741 attccttttg ctagaacgga agcgacaaca ggaactgaaa aatgttctga gaattctttg
     4801 atttgcggaa atccgtttcg cgttccttga ccaacataaa gaactggttt ttttgctttt
     4861 tcaagataag gcaaagcagc tagcaaatct ttttcttctg gtaatatcac acttgttttg
     4921 tgagtgtgag cggtggagaa aggttgttcg tcaatttctt caaaaccaag atcaacaggg
     4981 atagtcacga tagccacgcc tttatgctca taggcggctt taatggcttc atctactaca
     5041 tgcggaagac tttgtggtgt catgactgta cgattgtata cgctaacatc tgcgaaaata
     5101 ggattttcat ttagttcctg gaaagagtta tagttcatgg atgtagaagc aacttgtcct
     5161 aagaacgcta ctagaggcac atggtccatc tgtgcatcat acaaaccatt gatcaaatgt
     5221 gtcgcaccag gtcctgcaga gccgaagaca gcgccgactt tccccgttaa ttttgcatct
     5281 gcagcggctg cgagtgcgcc gacttcttca tgacgaactt gaatgtagtt gatttcggtt
     5341 ttttcttgat atagcgcatc cataattgaa ttgaaagatc ctccgggaat gccgtaaata
     5401 tggtcgatac cccagctttc aagaactttt accattgcga ttcctgcatt gatttttgac
     5461 atggtgtttc actcctttag caatgatgat acataaattt tttgtatcta gagtatagaa
     5521 ctttttatcg aaaatcccaa ctaattcgtc ttttcacaaa aatgtagtca tgtttttggg
     5581 aatatgctag agtagtacat agatagattc aagagagggg aatggcatgg ttgaatggct
     5641 attgagactt gaaccttggc agcaagcact tgctgggaca gcttttactt attttatgac
     5701 agctcttggt gccgggttgg tttttttctt taaagaaatc aaaaaagaag tgctaaactt
     5761 gatgttagga tttgcttctg gtgtcatgat tgctgcaagt ttttggtctt tgttagatcc
     5821 ggctattaca aaagcagaag aaaatggaga tattgcttgg ctagttgtaa gtattgggtt
     5881 tggtttaggc ggtgtgtttt tatatatggc agataaaaca ctgccccata tgcattttgg
     5941 accacaacat gaagcagaag gattgcctac ccatttaaaa cggacgatct tgcttgtttt
     6001 ttctattaca ttgcataaca ttcctgaagg tttagccgtg ggggtagcat tcggtgcagc
     6061 agcaactgcg gataatccga cagccgctat attagccgct gtttcggtgg cgctaggtat
     6121 tggtatccaa aacttcccag aaggagcagc tgtatcaatc ccgttgcgac aagaaggtct
     6181 gagtcgtaaa aaggcctttg tttacggaca agcttctgga atcgttgaac caattgctgg
     6241 agtgattgga gcgctactag taacgaaagt ggagctgctg cttccttatg cacttgcttt
     6301 tgccgcagga gcaatgattt atgtcgtggt ggaagaattg atccctgaag cacagcagac
     6361 attgtccagc aaacgtcatt ttgcagtttt tggcgtcatg agtggattta tcattatgat
     6421 gattttggat gtggcattag gataaataaa aaaaatatat atgatatatc aaatagggcc
     6481 gcgaacttat atcgcgaccc tattttttta gtaagttata tcataatgaa gtgaatggta
     6541 tcatacatta ataatagttt tttctatgtg tatttccttt gatccagcga acaagccaga
     6601 tgataagtgc gattgggatt agaactttga ccgcaaacca taaaagcccg cttaggacac
     6661 tgacaaaaag actgatgacg aagctagctg caattagcac taaaagaacc atgaagatat
     6721 ttaagaacat atttttttga cgcatacaaa accctcctct tgttcattct ttactataga
     6781 aaaagtataa cagggattgt ttaaaaacac ctccaactga agactgattt ttacttcatc
     6841 tacttaaatg atcaaacaat tgctttttct ctggtttatc atctaaaata gagcggaaaa
     6901 ggaggagaat atcatgccta aaaatcgtct tgaagcattt acagatgctg taatcgcgat
     6961 tatcatgact attttagttt tagaacttca tgaacctaac ggagacacgt gggaggcgct
     7021 cgctgcatta ggttaccgat tttttatcta tattgtcagt tttatcattt tagcgattta
     7081 ttggaacaat catcatcatt tatttcaagt tgtacataag ataaatggac gggtcttatg
     7141 ggcgaacagt ttctttgttt ttgcgctttc gctttttccg tttgcaactt catggatcag
     7201 tgagtacatc aatagcctgg ctccagaaat gacttatggt tttgttacct tgaccgctaa
     7261 tattgcgtat tatatcttgg caaaagaact tgtcaaagca aataaaggcc acgagaaagc
     7321 attgaaggcg atgttgaata attacaagaa atcgtatcta tcgattggtt tgaatgtgtt
     7381 aggaattgtc ttagggtatt tagtagcgcc agtagccgtg ctgatcgcaa acatcgtgat
     7441 tctagctgct tggatcatcc caagtcgtac catcgaatca caatatcggt aaaatgatcc
     7501 gtgtttaaaa tttaagagaa aaatatagaa aaaataaata aacgatcaaa tatccgagtg
     7561 atactagtat tcaaagaggg atgcagatgg atcaaaaaag aattcatgta ggactgatta
     7621 ctagttttgt cacgatgctt atttatttgg atcaaggtca tttgacttgg acagtagttg
     7681 gtgtcgtagg atttttgaca ttattcacag caataaaaaa ataaacatct ttatgtaatc
     7741 tgatgttcta tctatttaag aaaagcatgt ttacagcagt atgattctgt tgtaaacgtg
     7801 tttttatttg tggaaaattt aatttttaga aaagtgtatc gaaaaataga tagttagttt
     7861 ttccacagtg gataacttcg taagagataa aaataaaaaa ttagtcaaag aagggcgtat
     7921 agatttcttt tgataggaga tatacaacag aaatgaataa atattaacgt gttttcaaac
     7981 agtataaaac ggtgtggttt ttggaataaa aaaataacat atctcatttt acgcttttgt
     8041 tattttatac ttaaaaaata acgacacaga aaataccgtg tctcagattt ctctactact
     8101 tctaaaaact actgtttttt tcctttgaaa tgccttcaaa aagtaggatc aggcagaata
     8161 tactggcaga atcaaggaga agggagccta aataaggaga aaaaccatag aaaatataaa
     8221 gtaattactt ataaaacaag ctttttatag aaaaataata tacctgagta gggtataaat
     8281 taagacggaa ttcctttcta gattgagata ttgactagga agaatgttaa gattttaagc
     8341 ctttaagcta aaaaaaaacc ctttttgtta aataagggga atatagcctg tcagtttcaa
     8401 actgcaagtc gtcctgctgc aataatatag tagattagag atgaaaatag atttttttaa
     8461 accaatgact gaataaaaag accgtcatag atacacattg gaatgtgtct attacggtct
     8521 ttttttacga acgattttat tgaagacgga cagttcgggt caaaaataaa aaagctacga
     8581 tcagaaataa gaataaaatg tatcccaaaa taatggctag acggtctaac ttatttgtcg
     8641 atagatgttt gtttagaagt tgataggccc ataaacctat cagtcctcct gttgtattag
     8701 ttatcaaatc agtgatgtcc gctacgccaa taccgaatag gaattgaatc aattctgcca
     8761 agaagcttac aaggaaaata atccctaatt ttttagaaaa agaaagtctc ttgaaattga
     8821 cattcaaaag caccccaaaa ggaaaaaaga caatcaagtt ataagtggtc tcacgtaata
     8881 ccgtcgtatt ttcaaaagaa aaagtagaaa aagggacgag attgacattg ctgtaagaat
     8941 aagtgagaaa ttgtggaaga ttaaatgata gcttgaataa aatcaaccat aatagaaaga
     9001 ataaataaat ccctaataaa aattttgata cataccgcat agacatctcc tccatttaaa
     9061 aagtatacca tcgaaaaccg ctgaaaaatc ataaaaaagt atgaagttta aggaccaacg
     9121 tagttatcgt acgaaaatta gaagcttttt tttgttggga atccggtttc tttaacaaag
     9181 ctaagatagc gagttgcttg gaaaagttgc tatctttttt ctgtcaaaac gactcggaat
     9241 gttcgttttt tgttctaatt tctccaagtt gtttactttt tgatactaag tttatgaaaa
     9301 atcaagaaat agttgttata ctaggtaatg ctgacaaacg ggattggcat ttcatcttgg
     9361 ttcagctttt tttccaatta ctaggaagga gggatagatt gttcaagact tattttcgtt
     9421 tcaataaaat acaagataat ccttttcgta tcatgaatct gatgctactt atctttattg
     9481 tattatggtt aggttacata aatcatgatt ttttgatttc aagtttttca tcattaggga
     9541 tctttacttt tttctattat caaaacatac cgatgaaata tttgctgaaa cgtatgtttc
     9601 tgatcggttg cggactgttg gttgctttcg ttttggggat cttatcgact tatgttttat
     9661 ggcttgaacc atttgccgta gctttggttg ctttttccag cagattcgtg ttgcgattat
     9721 tccatatttc taaaccaggt ggcttgtttt ttgcgatgct ttctgccatg ggaacgagca
     9781 tgcagctgcc aatcgctcaa ttaccgatcg tctcacttta tttttttatg ggtgtcgttt
     9841 ttgccctgat tgcggcagtg attacaaaat tactggattc acggcctgag cagacaattg
     9901 aaaaagcaac tttaaaagaa agatttcacg aagagccttt agtgatcatt gattctgttt
     9961 tctacagcgc tgccttattt ctttccgttt atgtcagtca cggcttgaat ctgcataatc
    10021 cttattggtt gaccttgtca tgtgcatcga ttttgctagc agaaaacttg gatgcgatga
    10081 agcatcgaca agtccaatat ctgattggat cgatgggtgg gttatgtgtg tcggcattct
    10141 tgtcatttgt gccgtttacg cagttgcaga ccatttttct gatcactttt ctatatggaa
    10201 tcgcacaatt tctagttgct agaaattatg cagttgctaa tatttttctt aatccgatgg
    10261 cgttgatgct atctacactt attcgtggag catacttgat ttctttgatc gaatatcgtt
    10321 ttttagggat cgtcatagga agtttcatcg gtttaggtgt tgcgtgggta atgacggttg
    10381 gtttgcaaca ttatttaacg gttgttcgtc aaaaattaga atagaaggac aaatatatgt
    10441 gccaaaagag gttatgacaa gatttttgtc acaacctcta attcaaggtg ttcaaaagcc
    10501 agattctcag cattaagtca aattcacaca aaaatgtgga aaggaacttt caaaaaaatt
    10561 ttatatttat tctatcaaaa aattttgttt atgatggtaa ctgaataaac tttcctgaga
    10621 tggtcaatgc tttccttgaa agtcttacag atattctttc ttcatagaaa gtttaagttt
    10681 tatagttagt gtacaataaa agtatgttta taccatatgg tgtccggata atcagtcatt
    10741 taaacaatga ggttcaaaga tatgaaaagg ggaaatttga tgaagaaaat aagcaatgaa
    10801 atccaaattc tggcagaaac tttaactctt cccaaagtcg ttatagagca aatgattgta
    10861 caagcaaaaa acatccttga tgaggtgttt gagcaatata gtcatgaact aatggatcct
    10921 aataaagcaa aggcgactgc aaagaaaata gacaaagtct atgaagagga accttttgca
    10981 gcattagcta tttatctata tgcagcttct caaagttgga aaaaaattta tcaaccattg
    11041 aagattccaa gaactatcta tttggcaaca atgaatgctt ttactagatt tattcatgaa
    11101 catttccaag taacaggaag ctatcgcttt gaccgtggtt tttggatttg gcattacact
    11161 agtggcttga tctttcggat tggtgaattg gagtacgaaa tgacatattt tccgaaaggg
    11221 tacacggatc taagattaga aggaaagcag tgcctttctc ttcatattcc ttccgatgca
    11281 gatttatctc agaataagat aagagatagt tacgaaagtg ccattcgttt ttttgaactt
    11341 tattttcctg attaccatta ccaagtgatg tacacagata cttggttact atcacccaat
    11401 cttactaaat ggttgaaaaa agagtcaaaa atctgtcttt ttgcagccga ttatcgatta
    11461 ttatcagttg atgaacagga cgatagtgga gtcccttgga tctttggacg agtagatgcc
    11521 caaatacatg attatccaga aagtacgagt ttacagcgac aagcaaaaga gcaattgctt
    11581 gcaggcgagc acattggctc tggcttaggt attcttactt cataagttta gtacttcgaa
    11641 atagtttaaa agagcgtgag acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
    11701 tataaacggc ggaagcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
    11761 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
    11821 cttttttagt gttctatcga acacacagta cgttcaatag gtaaactagc tataacaata
    11881 gatattaaag ttaacggaca agagggcctt ttttaggtag ctaaaacgta ataatgaaaa
    11941 tttatttcca gagataggag tcaatcagct agtctgatcg actgttttaa caagttgact
    12001 ttcattttaa cttttgatac catttaaaac acaaatcgta attgtttcga tttgtgtttt
    12061 ttagcatgac tacaatcaaa gaagaaagtg ggtcgtccat atggaaaaaa cagatatctc
    12121 aagtgcatac cgccgcttga aaagcccaaa tatcaaaaca cgaaaacgtg cgctgaagat
    12181 catcaaagat gtgaaacgga atagtgggaa aaggtaagaa gaaggaaact taattaaaaa
    12241 aatagccgaa aaacgttttt cggctatttt caataatcgg tttttgtgta aggtttccga
    12301 aagaactgct gttgattttc catttttaac atatgataaa ttggtagaat ctttcttttc
    12361 aaattgatac cattccctaa ttccagttat atagttaact acagaaccac aaagcacgta
    12421 cgtttataaa agtattatct gtagttattt ttcaattccc tttcaacctc ttattttgac
    12481 cagtagtatt gatcacttta ccttttaaca cctttgttcg tttttgaaac atttttagtc
    12541 aagaatggaa acgtaactac ataatcaaag gttttgcgaa atatttcatg aaatatttca
    12601 tgtagaagcg agcaatacgg ttgcaaataa ccattatcgt ttaataagca gttacgaaag
    12661 ctcccaatat ctctaacgtt cagctcactc catttctttt cttcaaaaaa atcagtgatt
    12721 cgtatctctt tctatttgtc gaaaaagtta actttaaaaa gtaacgccga aaaaatatgc
    12781 aaaaaaataa aaaagcagtt actattgcta gaataacatc aaggaataat aagttttggt
    12841 tttcagataa tagtcgataa gttgtgtatt ggaattttag atacagtaat aaagtatttt
    12901 tcatgaaatt ttgtgagaat cactattaca agtatcttta ataaaattaa tggttaagga
    12961 ggaacttaaa gtagaatgta aatgaatagt tgtagtcagt cgattgtgat actagactca
    13021 aagttgcagg aataagtttt tcaatttgtg attggaagat aaaaaatgaa aaagaagggc
    13081 ttttttttat actttgttga ttgtagtgtt tattttccct ctgtttagcc aagtaacatt
    13141 ttctatgact caaccagttt caagtgagga acatgataac ctttttcgtg ctacagaaga
    13201 gtttaagaaa tatcaggaaa atattgttcc ggagactcca ttagctagtg agccaattac
    13261 tgatattgat ggaaaccaaa taggatggta tatacagtac gaagttatgg tggtaaataa
    13321 tgactcaata gtaaaaaata cagtaggctt agagctaaca ccgattgaaa aggtgagttc
    13381 attgttaacg tatatttata ctgatgaaag tgacgagact gagatcattt taacggatta
    13441 ctcaaaaatt gctactgata aaaagtatat attgtggatg tagacactag tgaaatactt
    13501 tatgatatag aaattgatga aaatttacta gatgtagcac aagaaatcga tcatcaaaag
    13561 caggaagcta ttcgatatgc tggtagaaaa gggaaaagaa aatatttatt acgtgaatgt
    13621 agcaaaagtt agagaagatg aaaatgagtg gaaagaattc aagagtcgct actcaattaa
    13681 ctcgactccg acatttacag tttacagaga aggatcgata gaaaaaactg ttttttggac
    13741 taaagaaagt ggaatgtctt tagctgaagt agaagagttt ttggattatg ttagtatgca
    13801 acagtaaggg agttcttatt atgaaaaatt ttgacacata ttatgttatt gttttttcga
    13861 tatctttgct gttaatatta ctaactttaa tgcagtataa agagtatcca gagtatttta
    13921 ttttaacttt tgtatattta gtaagtagct acacacttct atataaattc agaagttctt
    13981 ctagagtttc taaagtttgt ttcttaatta gcttattgat tctataatat ttttgattta
    14041 tataggaaat taaaaatatt atagaatcaa cactcagaga acgggttggt ttttaataga
    14101 tttaataagt actgataatg aaatttctcc tggttgggaa aacgaagttg gttaccctgt
    14161 agaagaattt tttcaagatt actttgcgga gtatgccgat aggataatga gagattacgt
    14221 aacagataaa actcaagaag tcatcaatca atcccagtca ttaaatgaat tatctgataa
    14281 ggcgaaagaa gaagcagttg ccattatttc ttcagggatt agtgactata ttgatatgag
    14341 tggtgaaatc aattatggcg ataaagacaa aattgtacgc atgataaaag cttttatcaa
    14401 aacttttgat gtgaaggcag atcttaagtt taatgatact tacctagtag gaaaattaat
    14461 taatatcctt gggacagaga aagatacgga aacgtttatt tcctctttgt ctatgttgtt
    14521 tacagataag catttttcaa ctcagcttgg tggagaggaa ctagtggaaa tcttttaacc
    14581 attacgccca gcattaataa aagtatatca aaaaagtact cctgatgatt tttaagattg
    14641 gtttgagaaa taatgtgaaa acactatttt taatgttaat tatagataga gatgaacgcg
    14701 tcatatggtt aaaagaatga aaaagtcaaa gatacactgc tatatttcac taaggatata
    14761 gcagtgtatc tttcatgtcg tttcactggc agttttttat aaaaggaggt gtgcatttca
    14821 gttgcttttc tttggtagtt agttgaatcg tttaagaatt aacggatgtg gcagacaggt
    14881 gctctgtcca aaaaccagta aaatctttga tggttccatc tttatttagc tgtgcctgtt
    14941 caaatactgt tttgttttta ccgaatggat caattgcaat aattgttccg atccccgcac
    15001 gtttggcagc aagtaatcct gagtaagcat cttcgataac aagacaatca gccggagcta
    15061 agctcagttt gtctgccgct ttaagaaaaa tatccggttg cggtttccca gggaaagagc
    15121 cgtcgtcata gacaatcttg tcccagtcaa accagcgggc taaatcgaaa atatcaaaat
    15181 aaaaagcaac attctctttg acagtagccg tggcaatcgt taatggaatc tgttggtcag
    15241 tcagctggtc aagagtcgct tcaagccctt ttgtcaactg aagctcgttt tcattttgca
    15301 agcaaagcat acgataatag ctttcttttt cttctgacaa tacggcgatc tcagaggcgg
    15361 tcaaatgctc tgaaatgaaa tgtttcaaaa tctccgtatt cgtccgacca tgaatatttt
    15421 ttaaaatatc ctcgtctgat aaaccatctt ttgtatgatc atgtatcata tgcagccaag
    15481 cagcttcatg cagatgagag tccaaaaaca ttgttccgtt gaaatcaaat ataatccctt
    15541 tcatagttac gctcctatag tggtattttc ggttgaatca gcaatttgca gatcgacata
    15601 ttgtgcatag tgctcttgaa cctcccgcga gatctggcga tccgtaatga gcagatcgaa
    15661 atcatgcaaa ttataaaaca cataaaaatc atctcgattg aattttttat tatcaacgag
    15721 tagatatttc ttacgggaat tatttagagc gatctgctgt gcttctcctt cttcggtact
    15781 gtaagtagag atcgcttcgt tgtgaacgcc atttgaactg ataaatgctt ttgtaaactt
    15841 tagtcttctt aaattgtcgt tggtgatcgg tccgacaaat gcgccggtat ttttccggaa
    15901 atcaccgcca atcagtagaa tttctggtcc atctgcttga gcaagaatat caaaaactgg
    15961 ataattattt gtaatgatcc gaattttctt gttcagtaaa tgcgtcgcca atagttcgat
    16021 tgttgtacct ggtcctaaga aaatcgtttc tccctcgtga atcaaggagg ctgctgtggc
    16081 agcaatttct tttttttctt gaatctgcag agtggctttt tcagtgtgag aaagttcttg
    16141 atctaaagtg taggaaagac tttgagcccc tccatggaca cgcagcagct tgcccgattt
    16201 ttctaattca tctaaatctc tgcgaacggt catatcagaa acatctaatt gattgatgat
    16261 ttcattaacg gtaaggattc cttttttatt gaccatttct acgatagtaa gcaatctttc
    16321 ttttttcaac attgtcttca cctctaattt aaggataatc tatcatataa acggacaaca
    16381 gtcaatctaa ttcgaacaaa agtaaacatt tagggtggtt tttatgtttg ttacaataga
    16441 ttttattgct gttttctggt ttttatgatt atttgtgttt gtttttgttg acaaatgttt
    16501 tggatgcgtt tacaatgcaa tcagttagaa agagcgaaaa ctgattatag atttgaggag
    16561 agatgtacat gcttaaatta ccagaagatt ttatttttgg cggagccaca gcagcctatc
    16621 aggttgaagg agccactaag gaaggtggca aaggcgctgt tgcttgggat gactttttag
    16681 aggaacaagg gcgattcagt cccgatccgg caagtgattt ttatcatcaa tatgcaaagg
    16741 acattgaatt gtgtgaacgt tttggtgtaa atggtctaag attatccatt gcttggagcc
    16801 gcatttttcc tgatggagct gggaaaccaa atccagaagg aatcgctttt tatcatcgtg
    16861 tgttcgaaga atgtaagaaa agaaacgtaa cgccgtttgt gacgttgcat cattttgata
    16921 cccctaaacg tttgtttgac catggagatt ttttgaatcg agaaacaata gaagcatttg
    16981 tttcctatgc cattttttgt tttcatgaat tcaaagaagt caaggtgtgg agtaccttta
    17041 atgaaatcta tccagttgca accaatcaat atttgttagg tgttttccca ccaggaatca
    17101 aatatgattt tacaaagatc gtcgcctgtc ttcataatat gatggttgct catgcccgtg
    17161 tagtgaacta tttcaaagaa aacgaacttc cgggtgaaat tggtgtagtt cactcattag
    17221 agacgaaata tgcggcaaca gacgcgccgg aagataagca tgctgccttt ttagacgatg
    17281 cattgtcgat ccgtttctta ttagatgcta cctatttagg ctattattca actgaaacct
    17341 tgactgctct tgatgagata tgtgaagcaa atcaggccag ctatcatttt cctgaagaag
    17401 atttcgttga attgaaaaaa gcatccacgc gtaatgacta cttagggatc aatcactacc
    17461 aatgccattt tgtcaaagct tacgatggag aaaatgcaat ccatcataac ggtacgggtg
    17521 aaaaagggac ctctgtttat aaagtgaagg gaatcggaga acgtatttat aaagaaggaa
    17581 ttcctcgaac tgattgggac tggctgattt atccagaagg tctatatgac ttgttgctgc
    17641 gaattaaaag cgattaccca cattacaaca aaatctatat cactgagaac gggatggggt
    17701 ataaagatca gtttgaagat ggcattatta tggatcagcc gagaatcgac tatttgagag
    17761 tctatttgga atccttaagc aaggcgataa cagccggggt aaatgtgaag gggtattttt
    17821 tgtggtcgct tatggatctt ttctcatgga caaatgggta taacaaaagg tatggcttgt
    17881 tttatgttga ttttgaaacc cagaagcgtt acccaaaaga atcagcttac tggtacaagt
    17941 tggtcagtga aaccaaaacg attatttaat aagtgataat ggaggaagaa aagatgaata
    18001 aagaggaatt acaaatgctt ggttttgaga tcgttgcata ttcaggcgac gctcgcagta
    18061 cattattgaa attattgaaa gaagtaagac aaggaaactt tgaacatgtt gattcggcgt
    18121 tgaaagaagc cgacgaaaat ttaacccttg cacataattc gcaaacaaaa attttagcgg
    18181 aagaagcatc tgggaaagag atggagatgg gattcatttt tatccatggg caggatcatt
    18241 taatgaccac tttattgctg agagatctga ttcaagactt tattgtactg tacagacaga
    18301 atcaaggata ggagaagaac gctatggatg gaatcattaa acaaatcgaa aaagggaaac
    18361 cattttttga aaaaatctca cgtaatagtt atttaggagc cgttcgtgac ggatttttaa
    18421 cagcgatgcc agctatttta ttttcaagca ttttcatttt gattgctgcg gttcctgaga
    18481 tttttggtta ccaatggcct gaatctgtct ccacatggct atggaaagtc tataattatt
    18541 cgatgggtgt tgtagggtta ctcgtttcag caaccactgc tcgctgcttg gctgattcga
    18601 tgaatcgtcg cttaccgaaa aacaaagtaa tcaacaccac ctctgtgatg ctggcctctg
    18661 tggtaagttt cctgttgtta agcgtctctc cgattgaagg tggattttca acggaatata
    18721 tggggacaaa gggactactg gcttcctttg tcgctgcatt tttaacagtt aatttatata
    18781 agttttgtgt gctgaaagat attaccatta aaatgcctaa agaagttcct ggaacgattt
    18841 cgcaaacctt ccgagatatt tttcctttct ctttttcagt atttgcggcc gttttaattg
    18901 atttaggcat tcgacaaatc ttccaaatct catttgcaga agcaatcatt acggtgctgc
    18961 agccgctatt tacagcagct gacgggtact taggaatcgc tattatctgg ggagcgatgg
    19021 cgctgttttg gtttgtcggt gttcatggac cgtcgatcgt ggagccggca attgcggcaa
    19081 ttatctatgc caatgtggaa accaatctgc aattgttcaa ttccggtgaa caagcttcta
    19141 aagtactgac ggtgggttta gggaattttg taggaactat gggaggaact ggggcaacat
    19201 tggttgttcc ttttatcttc ctactctttg ctaagtctaa gcagttgaag gcagttggga
    19261 aagcttcctt tattccagta agtttcgctg tcaacgaacc attgctattt gcggcaccca
    19321 tgattttgaa cccttatttt tttattccat ttttgttaac acctgttgta aacgtttgcc
    19381 tgttcaagtt ttttgtcgat gtattgggca tgaacagctt tatgtacgtt ttgccttggg
    19441 caactccagc accgatcggt ttggttctag gaacgggcat gggtatctta gcttttgttc
    19501 tagcagcggt tctaatcgtc gttgacttcc tgatctatct accattttgc aaagcatacg
    19561 atgctgtctt agtagagaaa gaagcacaaa aagcagaggt gatcgcaacc gaggaaggtg
    19621 cttctgccga tgtacgtgag gaagtacctg tggcagcgag tcaagttatc agcgatgcaa
    19681 ataaagaaat caatgtgttg gtactttgtg ctggcgcagg gacaagtgcg atgttagcta
    19741 atgctttaaa aaaaggtgca tctgaatcga aagcaccgat cagtgcaatg gcgggtgctt
    19801 atggttctca ttacgaaatg ctgcaggatt ttgatatggt ggtcttagca ccgcaagtca
    19861 attcttacta tgatgatatc aaagaagaca ccgatcgctt aggaatcaag ctggttgcga
    19921 caaaaggagc agaatatatc ggattaacga gagatcctga aaaagctgtt cagtttattc
    19981 tgaaccaatt tcaataaagg aggagccata atgaaaatgg gaatttggtt gcgtaaagct
    20041 gttttatttg cagctgtgtt aggggctttg ggggctgttt caaaaactgt tgaagcatct
    20101 gccagcgaat ttattcgcgg agcagatatt tcgatcctgt cagatatgga aaaatctggt
    20161 gcaaaatatt atgaagacga tatttcaaaa gatgccttag agattttaaa aaataatggt
    20221 gtcaactatg ttcgactgcg tttgtggcag gatccttata atagtaatgg agaaagttat
    20281 ggagcaggga caaatgatct ggaaacgacg attgaccttg caaaacgagc aaaaaatctg
    20341 ggacttaagg tgctgctgga ttttcactac agtgattttt gggttgatcc tgggaaacag
    20401 aatcttccta aagcatggca aggtctgacc tttgaagaaa tgaatacggc attgtacgac
    20461 tatacaaaga acgttctgtc ggaaatgaag cagctggacg tttatcctga tatggttcag
    20521 atcggcaatg aactgaatag cggaatgctc tggccttatg gcaagtcttg gggagaaggc
    20581 gggggagaat ttgatcgatt ggctgctttt ttgaagtctg gtatccaagc tgttcgagac
    20641 actcaaccta aaacaactcc agtaatgctc cacttagctg atggtggcga tacaggagca
    20701 tttacttggt ggtttgatga aatcacgagt cgaggtgtgt catttgattt aattggagtg
    20761 tcgtattatc cgtattggca cggttcaatg aatgaacttc aaaccaatat gaatgcgatc
    20821 agccaacggt atggtaaaga agtgatcgta gttgaaacag cttatgggca tacaacagcg
    20881 aatgccgata ctatgccaaa tgcttttggt gaagccgaag cagctgcggg gggatatgaa
    20941 ccaacaccga caggacaagc agagtatttg ctggatcttg ccgatcggat tcaggcagta
    21001 ccgaataatc gcggggcggg cttcttctat tgggaacctc tttggtacaa cggaaatgtg
    21061 agttgggcaa cacaagcagg tatgaattat ctcagtgttc aatccactat gggaaacgaa
    21121 tgggacaacc aagcgatgtt tgattttgct ggaaacgcct tgccaagctt gcgtgcgttt
    21181 aaacaagcag gggcgcaaat aaatctagtt aaaaatgcaa gtttcgaaag cgatggacca
    21241 acgaccagtc cttccaattg gcaaacctgg tttcaacaag gaaccgctaa gggtacgatc
    21301 aaaacggaag cgggcacttt ttttggggac tacaaactaa ctttttggca ggatcaagca
    21361 tacgaggcat cagcttatca aaatgttagt ggcataagca aaggaactta cacattgagt
    21421 gcctatgtaa tgtctggggg agagtttgat actaatcagt tatatatcaa aaattatggt
    21481 ggagcggaac taaatactgc ggttgttaaa acaagtgaat ggacaaaaat tacaatcaaa
    21541 gacattccaa ttacaacagg tgtttgtgaa attggggttt atagcaaggc ttcaggaaat
    21601 gcttggacga acgttgattt tgtttcattg gtgaaacaat agactgcgga tcgtttcgat
    21661 tcaagatcgg aggttccctt cagccacatc gttagcgtag aaggcaggaa tcgaacctgc
    21721 acctcgcctt tgccaaaaat aaccagaaac gatttccagt catttttacc aaaacgagta
    21781 aagcaccaga cttcatctac actttgtatt atgctaaaga gagttcaacg atttcgtaag
    21841 aagcaatgaa aaaattgcaa aaagttgtgc agataacata aagtttgttt aagaaagggt
    21901 aaccttagaa ttccgtcgtg aatatgattt gagcacttag atggatagaa aaaagttctc
    21961 ggaaaacagt tcgtttttcg agaacttctt tttatgataa gaatgaaaca aattaactta
    22021 tattatagtc tcttttggta gggaaccatc ttatttttaa tgaaaatcaa gcattttagc
    22081 tattgaagaa tgttgattta tagggattcc ccctccatat tatgttggct ctttattccc
    22141 actctgtttt caacgtaaag tgtataaaac agaatgcgat actatcaagg gagttaaaac
    22201 aatatacaga acagtaaaac actataaata aaaataaaag tgcaataaaa gtgcaatttt
    22261 tgcacctaat catactctgt ttatatatga aaactagatt ctgaatccaa tatttggtta
    22321 acgagaggcg ttcgaatttg aacgtctttt atatattact ttcaaataaa tatgaggtag
    22381 aatactatct attttctttt taacccaatt gttataat
//
LOCUS       DACDYM010000043        20808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDYM010000043 DACDYM010000000
VERSION     DACDYM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.043"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..307
                     /locus_tag="IWM59_001646"
     CDS             <1..307
                     /locus_tag="IWM59_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9979684.1"
                     /translation="AIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTK
                     ENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(731..901)
                     /locus_tag="IWM59_001647"
     CDS             complement(731..901)
                     /locus_tag="IWM59_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9979685.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1095..1274)
                     /locus_tag="IWM59_001648"
     CDS             complement(1095..1274)
                     /locus_tag="IWM59_001648"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979686.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1661..2773
                     /locus_tag="IWM59_001649"
     CDS             1661..2773
                     /locus_tag="IWM59_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9979687.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2851..3144)
                     /locus_tag="IWM59_001650"
     CDS             complement(2851..3144)
                     /locus_tag="IWM59_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9979688.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3312..3626
                     /locus_tag="IWM59_001651"
     CDS             3312..3626
                     /locus_tag="IWM59_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9979689.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3647..5626
                     /locus_tag="IWM59_001652"
     CDS             3647..5626
                     /locus_tag="IWM59_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9979690.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5639..5965
                     /locus_tag="IWM59_001653"
     CDS             5639..5965
                     /locus_tag="IWM59_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9979691.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5981..6499
                     /locus_tag="IWM59_001654"
     CDS             5981..6499
                     /locus_tag="IWM59_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979692.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6499..7851
                     /gene="celB"
                     /locus_tag="IWM59_001655"
     CDS             6499..7851
                     /gene="celB"
                     /locus_tag="IWM59_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9979693.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7935..9440)
                     /locus_tag="IWM59_001656"
     CDS             complement(7935..9440)
                     /locus_tag="IWM59_001656"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9979694.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9532..10122)
                     /locus_tag="IWM59_001657"
     CDS             complement(9532..10122)
                     /locus_tag="IWM59_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9979695.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10301..11947
                     /locus_tag="IWM59_001658"
     CDS             10301..11947
                     /locus_tag="IWM59_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9979696.1"
                     /translation="MDNQRKPSLDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11996..12715
                     /locus_tag="IWM59_001659"
     CDS             11996..12715
                     /locus_tag="IWM59_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9979697.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13194..14141
                     /locus_tag="IWM59_001660"
     CDS             13194..14141
                     /locus_tag="IWM59_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9979698.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14153..15073
                     /locus_tag="IWM59_001661"
     CDS             14153..15073
                     /locus_tag="IWM59_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9979699.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15114..16571
                     /locus_tag="IWM59_001662"
     CDS             15114..16571
                     /locus_tag="IWM59_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9979700.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16916..17530
                     /locus_tag="IWM59_001663"
     CDS             16916..17530
                     /locus_tag="IWM59_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9979701.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17523..19247
                     /locus_tag="IWM59_001664"
     CDS             17523..19247
                     /locus_tag="IWM59_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9979702.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19244..20695
                     /locus_tag="IWM59_001665"
     CDS             19244..20695
                     /locus_tag="IWM59_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9979703.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6711 a         3511 c         4168 g         6418 t
ORIGIN      
        1 ggcgatagat ggcttagcac caggagcata tcagctggta gaaactcaag cgccaacggg
       61 gtatgaattg gatgcgacac cagtaacttt caagatcgaa aaagaacaag aagtggcgat
      121 ctttttaaca aaagagaata gaaaagttgc tgatagctca gatataagag gaaacaatcc
      181 aacaagcagt ggaaataagc atcttccaaa aactggagaa accatcatta gccagtttat
      241 attgtcaata ttgggtgtgc tacttgtttt catcagtata aaatttagaa agaaacgaaa
      301 tatatgagct aaaataaaaa attgaataag catagttttt aaaatcaact agtacccatc
      361 aattgaatgg aaactaccga ttttagaagg ggtatacaaa caaatattag tatcggagta
      421 gagaaggttg aacccaacca gtaaataagt aaagacaatt ttgtgctgcg ttgagaagtg
      481 aaaaataaac ctttgtagta atactagtta ataaaaaata tacaaaaaca ctgcgaaaat
      541 cattcttgag tttcgcagtg ttttttgcct aatgactcaa gtttattggg taatactttg
      601 attttctttc tacaaataat cagcgatcag ttgtaatttt ttctctagtt ttaatggctc
      661 tttgtcaata aggagaaaaa caagtcgggt cttttgtctc ggaaagcttc aaccaactcg
      721 tgaataaagg ttacgcctcc ttgaaaggcg tggaaaaacc aagtaatcga tcaatcacca
      781 atttcgcatt tggtagaaca tgtttggtga gctgaaagta ggtggcgttc atgtctgtaa
      841 ctaagaattc tatttcttta ggattagtgc agctaaggaa atagtttgtt aatcgaggaa
      901 ttttttgtgt aggtaaattt tctcgattaa tttttctgtc tcgccatccg cggaaataaa
      961 actcagttta tcctcaacgg aaacgtgaga acgaaattca caaccctcaa cactcaagac
     1021 agaattcttt tggaagactt tggtaaatag cttctcaact ctttcaatgt gcgaataacg
     1081 gttgtcaaag atacctaaca acttttcgaa ataaaaaata acgacacttt ttcagtcagt
     1141 aaagaagtga ttttatgtct gacatgattt gaaatcgagt gccttggacg gataaaataa
     1201 ctttgagcgt tccaatggct tcgacagttt ttacaggtat agcgctgctt ttttaggcgt
     1261 ataaccattg gcatatgatt atattattca aagtggacaa tggtttcctt cttcttgttt
     1321 ttcaccacca ttaatttcct atttccatct accacagcag aaccacaatt tctacagaca
     1381 catggagcag ggaagagaac tgcagcaacg atcaatgttt ttttcttctg taaggtttcg
     1441 taagagacct ctgtaatcat caaatttttg tctattgatc tcagaatttt tttgatagaa
     1501 ccattcataa agcatatcgt tctctcagtt gtttattttg tggtgattta atcatacaag
     1561 aaaacgatat gtttttttat aaaaacatga ataggaaaaa tagttgacaa gaaaatgaca
     1621 acgccatatg atgttccata gtatttacaa cctgtcatag atgactgtaa aaaataaaag
     1681 taaaaagaaa ggacgacaac aggtggattt ttatcagtct ctacaactag atccgtttat
     1741 tttaaaacaa aaaatacgag aagcagcttc taaaaaagaa aagtgcatgt atatttgttc
     1801 attgttttta cgatcattat tcatcgtatt gtttgccatt tgttttatca ttataattac
     1861 aacattattt gaatcgaagc ataaaccata cgctgtggtt ttattctgta tgttgatgag
     1921 tattcgtttt gttgactttg gttataagat ttcccattcg atcataggtt tagcaatcgt
     1981 catgttctca ctattagtag caccgtatgt ccagctgatc aaatggtcag ttatgggcat
     2041 gttgattcat tttattttac taagcagcat attaatggca accgctagtg atcccaaaat
     2101 ggggaatgct tctttgtacg gcttttcata tctctttatt gtttattcgt tacctaaaga
     2161 ttcgttgaac aagaatttct ttacacaaac aagtagctta ttatttttgt ttttttgttg
     2221 gttttcagtt ctcctttaca gaaaacatcg agaaaagaac aaagacaaaa gcttgtttaa
     2281 agagattttt ttgaaaggca tgtattcgca ggaaaaaatt tggatgctca tctatgcatt
     2341 tggcattagt cttttgatcg ttgcaggaga gtatgtccct tttcagcgat tgatgtgggc
     2401 agggtttgca ttttcttcta ttgtatctag ctatggattg atgtcgattg gtttcaaaga
     2461 acgagcagta gaccgaatca ttggctcatt gattggatgt gttttattta ttggtatcag
     2521 ccaatttatt ccttttaact gggtcggtat attaggtggt ctagctttag gtgtttgttc
     2581 tacctataga tataaaacag tttttaattc ttttggtgct ttggcaatca ctgccagttt
     2641 attcggggtt ccaggagcag taacgatccg catttttgag aatatccttg gggtatgttt
     2701 aggaatcatg tatattgggg tgactgaaat acttatccga aaaataagag aaaaacacgg
     2761 tttgaatcat tagatgagat cgaataaaaa ataaggaaaa agtgaagata cacatggatg
     2821 ataaattgct atcattccat gtgtatcttt ttacggtaaa tcagtgaaac gttcgacttt
     2881 catttttaat ttatactttt tacggagttc gtttatttga gccatcattt cggatttgtg
     2941 atgcttttcg attgcttctt catctttcca acggtcgatc agtaaaagag attctggatc
     3001 ttctagtgga aagtaatagt catatttctc gtttccttct tctgcacgaa tcgctttgac
     3061 aatgtttctt tcgaccattt cttctgcaaa ttttctggct tgtccatttg tacttgtata
     3121 caaaatattc actgtgatac tcactaaatc ccctcctgaa aaaattctat cactagtata
     3181 tcacggtgcc agaaattatt attcaagata tgatacacat tttttccgca atgctacgga
     3241 aatatatatt ttgaatagaa aacgcttaca agatatgctt ggtttataaa aataaaagga
     3301 gtgattgatt tatgaaaaaa gcattgatta tttgtgcggc aggaatgtca tcctcgatga
     3361 tggcctcaaa gacaacggat ttctttaagc aaaaaagaga agaggtactc gtagatgcag
     3421 tttcagctac agaaggcgat aatatgatta agaatagtga ttttgattta tttctgatca
     3481 gcccgcaaac gacgatgttt ttggataaat tcaaaaaatt aggagaatct gttggaaagc
     3541 ctgttgttag tatccctttc caagcgtatg tcccaatccc tacaggcatc cagaagttag
     3601 cagaggtgat cgaagagaac atatgatgga ataggagtgg atgataatga aacagaagga
     3661 aaaattgctg ttgagatatt tgattgatca caagaaggag tatgtcacca gtcaacgttt
     3721 agcttctgaa ttgttattat ctgatcggac aatccgtaat tacttgcagc ggatcaaaga
     3781 gttagtagaa aaaaacggag gtaagatcat tgctaaacca ggttatggct accaactgca
     3841 tattttacaa agactgacat ttgacttgtt tttgagtcgg caagaaatag tacctggata
     3901 tgctagagaa ccgcaaggat tttatgagtc cgaagatcga caaaagtata tattgaacca
     3961 attgcttttg gaagatgcca ttctttttat ggatgatttg gcagaagagc tttatatcag
     4021 tcgttccagt ttgacaaagg acatgcaaga aatcaaggag cgattgattc catattcact
     4081 aaaaatcgtt tctaagcatg gtcaagggac atggatcgaa ggggaagaaa gaaatcgacg
     4141 gcattttatc atggatactt tttttggaaa ccattatgga aattcattga aagaatattt
     4201 agggcaaagc cgttttttcc cagacatcag ttttgaagag ctagtcatta tcattctcga
     4261 tgaaacaaga gaagctaagt taaaagtttc ggattttatc atacagaatc tgactttaca
     4321 cttggctttg gggatcaaac ggctccgaga agggtttgaa atcaaggaat taggcatcga
     4381 acaagaagtt tctaagcgag tagagtatca agtggcgcag cggatcgttc aacgtattga
     4441 ggtaatcgca ggtgtatcgt ttcccgaaga agaaattgct tatttgacat tacatctcat
     4501 ggctaagtcg aatcacaaag aaaatcaaaa tgatcaggaa ctatcaaagg aattagcgaa
     4561 agttctgaaa agattgtctc aggaatttca tttttcatta gtagaggatc tccagctttt
     4621 caatggacta ctcgatcatt tgtcaccgat gatgattcga ttgagccgag gtattcgcat
     4681 tgagaaccca ttgactgagg agataaagaa agaaaatccc aaagtctttg acgcagtaaa
     4741 aaggcatttc agcaacatgc cggctttgaa aaactatacc atcaatgaag atgaatgggc
     4801 ctatttggct ttgcatctca tggcggcatt ggaaaaagaa agagctgcgc ataaactaca
     4861 tgcattgatt atctgtgcaa cgggttacgg cagtgcccaa ctattaaaga atcgcgtagt
     4921 cagtgaattc ggtaaaaata ttacagtggt aagtgtaaaa ggctattatg agatcaatga
     4981 aacaacttta aaaggaatcg acctgattat ttcttctatt gatctttcga caatgttttt
     5041 caaaattcca gttttacatg tcagtatttt tttgaatgaa gatgatgttc gtaaaatacg
     5101 aaaggtagtt ggagaatcta taccctatca ttcctcagat agaccgatac cagcattttc
     5161 tctacagcaa aaaaagcaga tttacaccga acagttgtct gaaaaatttt tcaagactta
     5221 tacgtacgaa ccgaaaaagg aagaaattct tcatgatttg ctgcaaaagc tgagcataaa
     5281 tgaagaagaa ggatatataa gagaaatgcg aaagcagatc cagcaacgtg aaaatatggg
     5341 acaaatcata ttcagtgata cagtggcagt cccacatccc gctgttccag ttggaatcga
     5401 aacaaaggta gccgttgcat tgattccttc tgggatgcag tgggatcaat atcaagaaat
     5461 tcattttgtt tttttagttt cgccttctta tgtcgaaaat gaaggaatta cggtcgtgac
     5521 taaagcaatc gttaggttag ttgaccagct aaaagtacag caggaaatct tagctgaacc
     5581 tatttttgag aatttcagca gtaaatttat caaactgatc gaataaggag gaaaacagat
     5641 ggctgaaaaa ataaacagtg aaacactaca agtgacttct tttgatatca ttttccatag
     5701 cggaaatgca cggacactga tacatgaagc atttcgctcg atgcgtaaag agatgtttga
     5761 agaagtagaa gagaagttgc gacaagcaaa tgaagagatt cttcatgcgc atcagtcaca
     5821 aactcaactt ctgaaagaat atgccagcgg gacaaaaata gaaatggaaa ttatcatggt
     5881 ccatgcacaa gatcatctga tgacgacgat gacattattg gaagttgcac aggaaatgaa
     5941 ttatttgtat caaaaaaata aatagaaagg aaggatcatt gtgcaggata cagtaaaaag
     6001 gaaaatcgaa ttagaaaaaa agcaacttag tttgaaaaac atgtatttca atcgttatct
     6061 aatcgtacgt tacctgactg cctttttctt tttcatgaac atgcaatgga tgattctgct
     6121 tctatcagct aaaagtttag gaagtagttt accgatgatt ctgattttag ctattttgcc
     6181 tgcagtagga gaacaagtaa agctttatcg aaaacaccaa acgaacgttc catggacaaa
     6241 gtggtatttt ttgttccaga gtgtctttaa tttattagtg attccagtgt tgtttacctc
     6301 aggatttaca ttcttgtatc cttttatggc aaataacaat ggaggacagt tatttgtttt
     6361 cattcttgtt gtaagcggta tttttgccag tgcatttata cagtatcgtt taaagaaaat
     6421 ttcactcaac caagaccaac agtatatccg tattaaacaa tatgaaaaag cgttatattt
     6481 aggaaaggag aataactaat gtttgatttt ttgcagaaat acctgatggg acctatgggg
     6541 aaaattgcgc aacttaaaat cgttcgggct gtcatggcag cggggatggc ttctattcct
     6601 ttcacgattg taggctcgat gtttttggtt ttaaatattt tgccattacc gttcccattt
     6661 ttagaaggat tttttaatgc aacttttttc aaagttagtg acttgtacat gatcgtcaat
     6721 acaatgacga tggggatttt atctatttac tttgccattg tgtttgctta tgaattaacg
     6781 tcaattgaac gagatgaaca aaaattaaat gttaatccgc tcacgggggc attactatct
     6841 gtttttgcat tctttatgtg tatccctgaa ttgatcatga ctgatggtaa aatcagcttg
     6901 attgcttcta tcaatgagac agaaactgta gtaaatggta ttaggatggg ctcttttgtc
     6961 gaacgtttag gaacttcagg aatctttaca gctattatta tgtcgattat tgcggtcgaa
     7021 ttgtattgta tgtgtgtcaa gagaaattgg gtcataaaaa tgccagatgt tgttccgcca
     7081 ggagtttctc gttctttcac tgctctgatc ccaacatttg tcattgcgtt tgtggtgatg
     7141 atcatcaatg gattattagt tgcactagga acggatatct tcaaaatgat tgctatccca
     7201 tttgggtttg ttacgcagtt gacgaataca tggcttggtg tcatggtgat ttatttcttg
     7261 atccatgcat tgtggatcgt agggatccat ggagcgaata tcatcacttc tttcttgacc
     7321 ccgatcgtat tagctaatat ggcagctaac gcacaaggcg caaattatcc gttggcagga
     7381 gaattcaaca attcttatgt aaccgttggt ggatcaggtg cgactttagg attgatcatc
     7441 tttattgcat tcctcgcgaa atctgatcag ctgaaagtgt taggaaaagc ctcacttgta
     7501 ccaggattat tcaatatcaa tgaaccaatc attttcggga tgccaatcgt ctacaatccc
     7561 tatttagcgg ttccgttttt cttagctccg atggcttctg ctacactcgc ttattttgcc
     7621 attcggctgg aaatcgtcaa accgatgctt gcacagatgc cgtggccttc accagtcggg
     7681 atcggagcat ttgttggtag tggtggtgat tggaaagctg ctgtgttagc agtcatttgt
     7741 gcaatcatcg cattcattat ctggttccca tttatcaaat tctatgataa taaattatta
     7801 aatgaagaaa gagaaaaggc agcagaatta gctagtgaaa agttaaaatg atttctaaga
     7861 aaaaaggaac tgtgacaaaa aatcacagtc cctttttttc ttagaaatgg gaaaccacta
     7921 gtatataaat accattattc ttcaatcaac cgcttcacga ttgcataatc cccttcataa
     7981 ggttcatcta caccattgat tttttgatag ggatccacgc cttttcccgc aataacgact
     8041 gaatcttgcg gagcgctttc tgctaaagcg cgtcgaatcg cttcaggacg atccatttca
     8101 taatgaacga tcaaatctgg attagtaatt gcttcattga tttcttccgc gatttttttc
     8161 ggatcttcaa atgctgggtc atctgccgtc aaaaaggcga tgtcagctgt ttctgatagc
     8221 acttttccaa aatcatgtcg acgggagatt gctttgtttc ctgggctgcc taatacaaca
     8281 attacacggc catctggatg ttcatttttg gcaaatttca gcaatgtttt caagcttaga
     8341 taattatgtg catagtccac atagacatgc gctccattgg attggatcaa ttgatccatt
     8401 cggcctggta cacgagcttc ttttaatccg tcttgtgcat cttgactgga ggcacctgtt
     8461 agtgctgcgg caatgatcgc actggctgca ttttcttggt tgaatcctcc agccagcaaa
     8521 atatcataag cagtatcgca gatagctaag cgatcttcat gagaagataa agtaaaaccg
     8581 tgagtacctt tatctgaacg gatcacttga taattgcttt cttccgaacg accataagtg
     8641 atcgttggaa tctggaatag atcggctgtt tccctaagta aatcaaaata gtcactttca
     8701 tgatttaaaa caactgtttt tgaattcaag atcaattgtc gcttgcaata gaaatagtca
     8761 tcaaatgtcg gatgttcgat cggactgata tggtcaggcg agatattcaa gaagattccg
     8821 acatctaaag tcaagccgta tacgcgttgt gttttgtaag cttgtgaaga aacttccatc
     8881 actagatgag tcataccatt ttcaactgct tctgacatca tacgatataa atctaatgac
     8941 tcaggcgttg tcaaatggga tttgaaatat gttttgccat ctaacgttgt attcatagta
     9001 gaaagcaatg ctgttttttt atttgttgtg tgatccaaga ttgcttttgt aaagtacgca
     9061 gcagttgttt taccttttgt tccagtgaat ccgatcactt tcaatttatt ttgcggatag
     9121 tcgtaaaaag ccatagcaag aagcgccatt gctttacgga tatccgttac gatgattcct
     9181 actgctgaat tttcgtatgg ttgttctgat acataatatt gaataccctg ttcgattgct
     9241 gaatccaaat attcttcttt gaaattcatt cctttacaga aaaacaaagt agaagcatca
     9301 gcttttcttg aatcataact taactgaaga aattcagctt tttctggtac atcaagatgc
     9361 cagcctcttg ttgaaacaaa ttcttttaat agattttctt ttaatagtaa atgacggatg
     9421 tcttctaaag agaagctcat tgattgatcc ctcgctttat tcaatttctt ggtttattat
     9481 agcacactct ttttaatata tcgtttgttt ccgctatgac aaaatccagc tttattcaaa
     9541 ttttcttgac acaggggaca agcggatgag tattttcata aacaatgaac tgactgtaat
     9601 agataggaga acgaatagtg tctccaacaa tccaaaaaca taataattca aaaagacgat
     9661 aagactattg gtaagtaaaa gtccttgcgg cattggaagc cgaaaaaatt tgtggaaaat
     9721 aatcggcggt atcgttatac cgccatttga ggccccgact gtgtacaatg tgtaaacccc
     9781 aagactgaac aaaagacttc ctgaaatcag tgaaaagaaa tgtgaggaga tcatttccac
     9841 ttttggaaca atctctaaaa caacaggaaa aagcagactt cccgctacag tcttaaaaaa
     9901 tactgatttt cctaatagga cagcggctag aagcagcatc ataagattga tgctccaaac
     9961 agtaactgag cgttggatag aaaaggccgt ttcaactaaa tgtccgatgg cgcccactcc
    10021 ggcggaagct atatcgtgag ggagcaagaa ccaattcaat ccaactgtga taaagaaaag
    10081 acctaaaaat ataacgggta gtttttttat ttcttgtgtc attgatatct cctataaatg
    10141 ttagccataa aaggctgtat atttctttac tttaccaaaa aaacgaaacc gaattcaaaa
    10201 aaacaattgt tcttataaaa gttatataaa atcgattgcc agtttctgtt ttaccaatta
    10261 aaatgatagt aatgttttgt agaaagttgg aatctgaccg atggataatc aaagaaaacc
    10321 atcattagat caattaacac cgcaagaaaa aatggcacga ggctctgctt ggatgaccgc
    10381 cagtaatatc atttcaagat tgctaggcgc tgtctatatc attccctggt atgcttggat
    10441 gggcgaaaat gctaaggctg ccaatggatt attcaatatg ggatacaata tatatgcatt
    10501 gtttctactg atctctacag caggaatacc tgctgcgatt gcaaaacaaa ctgcacgcta
    10561 taattcattg aatgaatatg gcacttcacg acgattattt atccgtgcat tacagatgat
    10621 gggcgggtta ggcctgttgt ttgctttgtt catgtacatc gcctctcctt ggctagctca
    10681 tgcttctggt ggcggagaag aactgattcc aacgatgcgt tctttgagta tcgctgtatt
    10741 ggtttttcct tgtatgagtg tgatacgagg gtatttgcaa ggcaatcaag aaatgatgcc
    10801 ttatgcgctg tcgcagattg tagaacaggt ggcacgtatt ttttatatgc tattggctac
    10861 attcatcatc atgaaagtca tggatggtga ctatgtgacg gcggtcacgc aatcaacttt
    10921 cgcggcattt atcggtatgc tagcaagtat tcttgtcctt ttgtattttt tgaagaaaca
    10981 tcaagcgtat acatcggcgt ttattcacta cagcgaaaac aaaataaata ttgcaacgaa
    11041 ggaattgttg ttagataccg ttaaagaagc gattccgttt attattgtgg gttcaggggt
    11101 aacgatcttc aaattagtcg atcagttcac ttttatgaag atcatgtctg catccaccga
    11161 gtattccaat gcacagttac tggatctttt ttcgattttt agtgccaatc cagataagtt
    11221 gacgatggta gttattgcat tggcaacatc gatcgcgtct actgggttac cgttgattac
    11281 agaagctgtt acgttgaaag atcgagtagg tctggcaaaa ttgacaagcg gaaatttaca
    11341 gttgttttca ttttttatgt ttccggcgac atttggtgtt atgttacttg cctatccgct
    11401 aaatacatta ttttatacac cggattctct aggaagtaat gtactgatcc aagcaagttt
    11461 tgtaggactc ttcctaggac tatatatgct agtttccaac atgctgcaag gaatgtttga
    11521 aaacaaggcg gcaattaatt atttggttat tgggtttcta gtgaaactcc ttcttcaata
    11581 cccagccatt cgtttatttg aagtatacgg ccctttattg gcgacaatga tcggatttgc
    11641 tgtgtcttgt accttgatct taaagagaat ccatgaagta gcccgcttta aaccaagttt
    11701 tgtctggcgc aggacattgc tgatctttat tcttaccttg attatgcttt tggcttcgtg
    11761 gatcacaaag atgatttttg gtgctttttt aaacgaagat agcaaattcc agtctctatt
    11821 gttgattctt gtagtagcag gtataggggc tttagtgtat ggttatttag ctttgaaaat
    11881 ccgcttggca gaaagactac taggaccagg aatagcgcgt ttacgacata aattaaaaat
    11941 aaaataagtg aatcagaaga gctagattga tgctcttttg aaatggggga aacgcatgcg
    12001 tttagataag tttttagcag atatgggact tggcagccgc aaagaagtca aaggattaat
    12061 caaaaaagga acgatcgaag tcaatggaca agtggtgaaa agcgataaag cgcaagtaaa
    12121 tgaattcgaa gatgcggtta cttatttagg agaaatcatt acgtatcaaa aagattttta
    12181 ctacattttg cataaacctg caggagtcat ttctgctact caggataatc atgatcaaac
    12241 cgtaatcgat ttattggaag accaggatta tcgggaagat ctttttccag tcggccgatt
    12301 agataaagac acagagggcc tcttgatttt aacgaatgac ggggtgtttg cccatcagtt
    12361 actatcacct aaaaaacatg tggagaaaga atatctagct gaggttagcg gtataatgac
    12421 agaagaagat atccgtttat ttgcagaagg actgattatt gatggcgatg agcagacatt
    12481 gccggcaaaa ctccaaattg atcaagtaga tcaagagaca gaaacttcaa ttatccgtct
    12541 gattttgcat gaaggaaagt ttcaccaagt taagcgaatg gtcaaagcag taggaaaaga
    12601 agtcttatat ttaaaaagaa tccgtatggg aaaattttgg ctgcctaaag atttgaataa
    12661 agggcaatat cgacccatga ccaaagaaga gctagaacaa gtgagagaaa aataattgaa
    12721 tggatagcta aaagaataag cagagtagtt gtagcgccat ctgaaaagca gacttgtccg
    12781 tctgactttc ggatggcttt tttgtgaaat ttcggcttat ttccgtagaa gctacttctg
    12841 gaacaccgtt tgttcggaat aagggactgt gacaagactt ttgttacagt ccccattgtt
    12901 ccttgtgact cagagttaga tagtaaggac acgatctccc taatactcag gccaaaacgc
    12961 tgaattcaca acctctgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa
    13021 aacggcaaaa tatgaggaac tattttcccg tttttttctc taatactcag gccaaaacgc
    13081 tgatttcaca acctctccta tactttgttt cattttccct atactttgtt tatttattct
    13141 cattattgaa agcgctataa ttacgttgcg aatgaatcga aaggggtttt tagatgaaaa
    13201 aacgaaaggg gttcaatcat ttttttatga atctatggcg ttataaagca cttgtcctga
    13261 tggctgtacc gggtatgctt tggatgattt ttttctttta catacctgta atggcgaatg
    13321 tcgtagcatt caaaaatttt catatttcag cagatgggtt cttggctagc ttgagggaaa
    13381 gtccttgggt gggttgggaa aatttcaaat ttttattttc ttcggatcaa gcatttctca
    13441 tcactaaaaa tacaatttta tataatgtag catttatcct tttgaatctg ctgatctcag
    13501 tcgtatttgc aatcattatg agcgagttga gaaataaacg actcgttaaa gtttatcaaa
    13561 cgatgtcctt actaccttat tttctatctt gggtcattat cagttacttt gtttacgctt
    13621 ttttaagtcc tgacaaagga atattcaatc agtggatcac tggacatgga ggagaagctg
    13681 tcaattggta taatgaaccg aaatattggc cgtttatctt ggtgttcatt ggtacatgga
    13741 aagggattgg ttataacagc attatttact ttgcatctgt tatgggaatc gatccgactt
    13801 attatgaagc tgctatggtt gatggagcta gcaaatggca gcagatcaaa catgtgacga
    13861 taccacaact tattccacta atgacgattt taacgatttt agccgtagga aacattttcc
    13921 gagctgattt tggcctgttt tataacattc ctaggaattc cggtgcatta tatgatgtga
    13981 ctcaagtttt ggatacgtat atctacaatg gcctgacatc cacaggagat tttggtatga
    14041 cggctgctgc aggactttac caatcagttg tcggctttgt cttgttgatg atcacaaata
    14101 cgatcgcccg acgttttgat agcgattctg cattattcta aggaggaaaa aaatgaaaaa
    14161 aaagaaagtg gagagagtcc aagtccgctc tttcaataag acggctaatt ttttctctaa
    14221 tataatgatt gcactctttg ctatttcctg tattttgcca tttatatttg ttatcgccat
    14281 ctcttttacc gatgagtcca tattgtcgac tcacgggtac agtttttggc cagaaaagtt
    14341 cagtatattt ggttatacgt acttgttcga ccagatgcag gataaaattt tccaagcgct
    14401 tttcgtcact gtacttgtta ctgttttagg tacactgatc aatagcacgg ctacttcttt
    14461 atatgcttat gcgatttcac ggtcaaactt tccattccgt cgttttttta ccgttttttg
    14521 tttgattacg atgttgttct caccagggat ggtggccaat tatcttgtta tgacaaatct
    14581 tcttcagtta aaagatacga tttgggcatt gatcttgcca atggctgtca gtcccttcaa
    14641 tattattgtg atgcgcacat tctttaaacg ttcggtctct gattcgatta ttgaatcggc
    14701 acggatcgat ggagctagcg aactgcgaat atttgttcag atcgttttac cgttagctgt
    14761 tccaggtatt gcgacaatca gtttatttgc cgcattgggt tactggaatg actggttcaa
    14821 tgctttacta tacatccaag atgataagct agttccacta cagtatcttt tgatgaaaat
    14881 ccaaagtaat attcaatatc tgacacaaaa ttcaggagca ggaagccaat tgacaggcgg
    14941 attagcctca gttccaggag aatctgccag aatggcgatc gtagtcatct cgacgttgcc
    15001 tattgcgatc agctatccat ttttccagaa gcattttgtc aaaggtctaa caatcggcgg
    15061 agtaaaagaa taaagaaaca aaaatataaa tcataaaatt aggaggaaga aaaatgaagt
    15121 tatggaagaa actagccttt acaggtgttt cagcgttgtt gttgggaaca ttagcagcat
    15181 gcggaggttc tggttctaaa gaccaggcag aagctagcaa ttcggacact ttgcagatgt
    15241 atcaaatcgg agataaacca gataatttcg atcaattgat ggaagtagca aacaaacgaa
    15301 tcgaaaaaga gatcggtgta aaagtcaata tcaattacat tggctggggc gattacgaaa
    15361 agaaaatgaa cgtcatcatt tcttccgggg aaaattatga tatcgccttt gcgaacaact
    15421 atgtttccaa tgcccaaaaa ggagcctttg ctgatttgac tgaattagcg cctaagtacg
    15481 ctgaaaaagc ttataatgat ttagatgaag catatattaa aggaaatctg gtcaatggaa
    15541 aattatatgc tttcccagtc aatggaaatg tctttgctca acaagtactt acttttaaca
    15601 aagctttact agataaatac gatctttcaa ttgatggaat ccaatcttac gctgatgcag
    15661 aaaaagtctt gaaggaattc catgaaaaag aaccaaacac ggcagctttt gccattggac
    15721 aaggcttcaa agtccaaggg aactttgatt atcctttagg gaatacgtta ccatttgcta
    15781 ttgatctgga tggagatgat tcgaaaatca tcaaccaata cgataatgaa acgtatatcg
    15841 gactgttacg tacaatgcat agctggtaca aacaaggact cattccaagt gacgctgcaa
    15901 cgagcaataa ggattatcca ctagaaggaa atacttggct aatgcgtgga gaaacacaag
    15961 gaccgtatga ttatggcgat acaattttga caaatgcagc aaaacaagaa cttgtctcaa
    16021 aagcaattac tgttccacta aaatctacag ctcaagcaca aatggcaaat tttgtcgtat
    16081 caaatacttc aaaaaataaa gaaaaatcag tagaacttct agggctgctt aacagtgatc
    16141 cagaattatt gaatgggctt gtttggggga tcgagggaga agcatgggaa aaagtcgatg
    16201 gttcagatga taaaatcaaa ttgctagatg gttatcagcc aaatacacat atgtctgctt
    16261 ggaatacagg aaacaacaag attctatata cacaagagtc tattacggat gacatgattg
    16321 ccaaacgtga tcaatcgatt gctgatgcag aaacttcacc gattttagga ttcagcttca
    16381 acactgacag tgtcaaaaca gaattaagta atatttctaa tgtcatgaat caatatttag
    16441 atggattgaa tacagggact gttgatccag atgaaacatt accgaaattg aaagacgctt
    16501 tggacaaggc tggctatgat aaggtattga aagaaatgca aaaacaatat gatgagttcc
    16561 gacaagaata ataatgaata aagacaaagg ggttgtgaca aaagacgtgt cacaacctct
    16621 tgttccgaat aaacggtgtt caaaagccag tttctcggta ttaagctaaa tatatgtaaa
    16681 aatgtggaaa gccatattca caaaattttt cttactactc gaaactaagc ggctatttca
    16741 caaactcttg gtaaacggtg ttcaatcagc taaccctcgg tattcgctca caaaacgaca
    16801 aaatatgagg aactattttt ccgtttgttt ctctaataat caggtcaagg tgctgattgc
    16861 ccaacctttt tttgtataat gagaaagtat aaacttaatg tttggaggag aagatatgtt
    16921 agggaaggtt ttggaagagt tatttatccg gatctgggtc attataaaac taaccttgta
    16981 tttctggata tatacatttg ccggcgggat catctttgga ttaggtgcgg catggaaaac
    17041 agtcaatgaa ctattttatt tgtacggatt cgagtataaa gaaatcacaa taaaaagagg
    17101 ctggaatata tataaaagaa attttttaag aggcaatctg ctatttagtt tatttttatc
    17161 tggtactgct ttattgagtt acaatttata tttatctgtt caaatcaaag ggctcatttt
    17221 tttagctatt gattttattt tactttttgc cttgctctgc ctatttgcta cttatcaata
    17281 cagtatgatt ttagatagtg aatatacgat ctctgttcct aatctgctaa agctaagttt
    17341 tatttcggtt ttttccagct tttcgtcttt tctaaaaata attattggta gcggtataat
    17401 tttaggcgtg acttggcaat tcaaaggatt gatcctgttt ggtgtcattg gtcttttgac
    17461 ggtttggaat ggtacgatga caacacattg gcgtgaagaa ttagataaac agttggaaag
    17521 ttatgaataa gtttcggatt ctattttcaa aaggaacctt actaaatcgt ctgttgcgga
    17581 aatacgcggt tatcatgatc ggtgtaacga tgactgctac cgttattttt agtatccata
    17641 cttgggaaca aaatcaaaaa caggcagaaa acatgacaag tgatgccgtt caaagcacaa
    17701 gcagaatgct gaatgataaa acaaccttga gtaggatcat caaaaaccag cttgtaggga
    17761 attccgaaaa gatcgaaaat gtgacgacct acttaacaaa acccatagat cagtatctga
    17821 tgtatgtata cgaacagcaa aacagtaccg atgaattagt ttctttccca aatcaaatca
    17881 aagatctcta cgcaaattat gaagaactaa gtgcgatata tatcgtgttg aatcaactac
    17941 cggagtatta tgaatcttct agagaaaaca aaggtgggga aataaaaaag gggacaccaa
    18001 aattactaga cgccttttat atcaagttgc ctatcacaca aggaggagct gagtcaggaa
    18061 gtatttttat tggttttcaa aagaaagaac tagatatgat tttagaaaat ctaacgtcat
    18121 tcaatggact ctctttatac atgatttctg gaacgacaaa tcgactatac acattccatg
    18181 ataataaaat agctaaagaa acgtttgaaa gacaagaaat gatcatcaca gagagtctga
    18241 agaaaaattc acaattgccg attgacgatc tggaaaaaaa caattttgtt caacaccaag
    18301 aactgtctga tgattatcgt atattagctg tactagataa aaaaagtgta aaaatcgaaa
    18361 cgggaaaaaa tcttgccccg ctgatcattg gtatcattat tttagacaat atactgctta
    18421 tctttcttta taaaacattc aaacgatatt cacagcaagt atctatcgtc atgcaggcca
    18481 tggatcaaac agcagcaggg aatttagaga cgcggatcga cataacaaac acggaatatg
    18541 aactgaagga actttcaatc ggtatcaacg aaatgttgga tagcatcaat cagtttgtag
    18601 aagacatcta caaattagaa atcaaacagc aagacgcaca tatgcgggcg cttcaagcac
    18661 agatcaatcc acacttttta tataatacat tagaatatat ccgaatgtat gcaatcagcg
    18721 aaggaagtga agaattagca gatgttgtgt atgcgttttc cgctttgctt cgaaacaata
    18781 cgaatcaaga gaaaacgatc actttaaaag aagaactgga cttttgcgaa aaatatgtct
    18841 atttgtatca aatgcgttat cctaatcgag tagcctatca ttttatgatc gatcctgatt
    18901 tggaaaagat cgaagtacct aaatttgtga tccagccatt agttgaaaat tattttaaac
    18961 acggtattga ttttactcga ttcgataatg cgctaagtgt caaagtcttg caggaaggca
    19021 aacgggtaag gatcatcatc aaagataatg gaaaagggat gactgaaaaa cggctaaaac
    19081 agattgaaga aaaactgtct catcctaaag tggaacttca cggttctatc ggtctgcaaa
    19141 acgtgaatga acgtttacgt gccagcttcg gcagcagtta ttatatgtcg cttgagaata
    19201 atgagacagg tggactgacc gtttctatta cattcaagga gggatgagcg tgtatcaagt
    19261 gttattggta gatgatgaat acatgatttt gaatgggtta aaaaagataa ttgattggca
    19321 aagtttaggc tttcaaatag tagcaacagc agagaatgcc aaagaaggac tagctgtact
    19381 tgaacagcga cagattgatt tagtagtaac ggatgtgaca atgccagaaa taaatggact
    19441 ggaatttatt gaagcggctc aaaaagaacg tcataatttt gaatttatga ttttatcagg
    19501 ataccaagaa ttcgattatt tgaaaggagg tatgcaatta ggcgccgtga attatttgat
    19561 gaaacccgtg aataagttcg aattggttga aagcctcaag aaaatcaaaa cacggttaga
    19621 tcagcaaaat gaacaaaaaa atcaacaaga aatctatcaa gaactgttat tcagtcaatg
    19681 gttgaatgaa gaattggatg aaataagtga agaagaaata ataggacaaa taagtgagaa
    19741 gcagcatagg atagtattga ttcaactggc aagaactcat gatactctta tcaatagatg
    19801 gttgcaagaa cggcaacaaa tcttttacta tcaacgaaat tacggagacc gtgtattgat
    19861 cacgttgttg ttggaggcag atgaagttga tggcttctgt tgttttgttc aagaaaactt
    19921 tcctgatcag gaatggctga tcagtatcgg ggaagagaca gattctttgg acaaaatccc
    19981 tgaaacattc caacaagcga aagacaatct acagttgcat caattttatg gagataaaga
    20041 cgagcatgtt ttttacgcag tacaagcaga aacaaaagat caaaccatcg atttttcaag
    20101 tttcagacgc gtgctgcaaa ataaacaatt ggaagtcgct caaaaaatga tcactgattt
    20161 ttttgaacag tttcagttag cagccatgat gccggaagat atacgatatt ccagtttttt
    20221 actgtttatg gagattcaac gggaattgat tgatctggaa gatgaggaat atttacaagg
    20281 aatcgaaaaa atccatcagg caaaaacggt gcaagaactt cagcagctac ttctgtcatt
    20341 catcaaaaag catcagagac agaaaaagta cagtgacaat gtggaaaaag taatcgtaat
    20401 cctgcaccag cattatcaag aaccacttac actcaaagaa gtgtcagaaa acctgcattt
    20461 aaatgtcatg tatctaggac agctgttcaa aaaagaaacg aaaaaaagtt tttcagctta
    20521 tttgaaccat ttacgtatgg aaaaagcgaa acaactgctt ttgcacagta atcaaaacat
    20581 caatgagata gcaagtgaga ttggctacaa caacaccacg tatttctcta aattattcaa
    20641 gaaaatcgtc ggcagatccc ctaaagagta ccgagaaaac agtggagaat cataaaagaa
    20701 aaagccaatg taatgaaata acttataaaa aattgtctag aaatctaatt ttgggctgtt
    20761 cttattactt gaggcaaggc agctttttca tgacctctcg ataaacgg
//
LOCUS       DACDYM010000044        20664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDYM010000044 DACDYM010000000
VERSION     DACDYM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.044"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..982
                     /locus_tag="IWM59_001666"
     CDS             131..982
                     /locus_tag="IWM59_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9979704.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            1103..2968
                     /locus_tag="IWM59_001667"
     CDS             1103..2968
                     /locus_tag="IWM59_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9979705.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            3016..4425
                     /locus_tag="IWM59_001668"
     CDS             3016..4425
                     /locus_tag="IWM59_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9979706.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            4533..5234
                     /locus_tag="IWM59_001669"
     CDS             4533..5234
                     /locus_tag="IWM59_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9979707.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            5393..5872
                     /locus_tag="IWM59_001670"
                     /pseudo
     CDS             5393..5872
                     /locus_tag="IWM59_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            6720..7295
                     /locus_tag="IWM59_001671"
     CDS             6720..7295
                     /locus_tag="IWM59_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9979708.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            7295..7735
                     /locus_tag="IWM59_001672"
     CDS             7295..7735
                     /locus_tag="IWM59_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9979709.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            7751..8452
                     /locus_tag="IWM59_001673"
     CDS             7751..8452
                     /locus_tag="IWM59_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9979710.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(8940..9365)
                     /locus_tag="IWM59_001674"
     CDS             complement(8940..9365)
                     /locus_tag="IWM59_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979711.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            9739..10710
                     /locus_tag="IWM59_001675"
     CDS             9739..10710
                     /locus_tag="IWM59_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9979712.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            10742..12139
                     /locus_tag="IWM59_001676"
     CDS             10742..12139
                     /locus_tag="IWM59_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9979713.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            12298..12921
                     /locus_tag="IWM59_001677"
     CDS             12298..12921
                     /locus_tag="IWM59_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979714.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(13147..13671)
                     /locus_tag="IWM59_001678"
     CDS             complement(13147..13671)
                     /locus_tag="IWM59_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9979715.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(13664..14137)
                     /gene="tsaE"
                     /locus_tag="IWM59_001679"
     CDS             complement(13664..14137)
                     /gene="tsaE"
                     /locus_tag="IWM59_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9979716.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(14260..15240)
                     /gene="pta"
                     /locus_tag="IWM59_001680"
     CDS             complement(14260..15240)
                     /gene="pta"
                     /locus_tag="IWM59_001680"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9979717.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(15393..16073)
                     /locus_tag="IWM59_001681"
     CDS             complement(15393..16073)
                     /locus_tag="IWM59_001681"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9979718.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            16199..17068
                     /locus_tag="IWM59_001682"
     CDS             16199..17068
                     /locus_tag="IWM59_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9979719.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            17094..17609
                     /locus_tag="IWM59_001683"
     CDS             17094..17609
                     /locus_tag="IWM59_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979720.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(17656..18219)
                     /locus_tag="IWM59_001684"
     CDS             complement(17656..18219)
                     /locus_tag="IWM59_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9979721.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(18274..18453)
                     /locus_tag="IWM59_001685"
     CDS             complement(18274..18453)
                     /locus_tag="IWM59_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979722.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(18530..19039)
                     /locus_tag="IWM59_001686"
     CDS             complement(18530..19039)
                     /locus_tag="IWM59_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979723.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(19135..19515)
                     /locus_tag="IWM59_001687"
     CDS             complement(19135..19515)
                     /locus_tag="IWM59_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9979724.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            19845..20594
                     /locus_tag="IWM59_001688"
     CDS             19845..20594
                     /locus_tag="IWM59_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9979725.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         6544 a         3710 c         4166 g         6244 t
ORIGIN      
        1 aaaattcgat ggtatgatga ttttgaataa gaaatggatt gttactgtac ggcaggcaaa
       61 accagaattc agctaagaaa cagtaggcga attctaggtt tttttatttt tcttgagagg
      121 gcgaagcaaa atgaaaattg ataaaatttt gaataacaat gtcgtcattt cgaaaaatgg
      181 attcggggaa gaagtcgttt gtatgggaaa aggactagct tttcaaaaaa agattgggga
      241 cgagatttcg ccagaagcgg tccaaaaaga atttgtttta agagattctt ttgctaaaaa
      301 ccaattccag cagctgatgg ctgatattcc ggcagaggag atcgaatggg tcaaacaagt
      361 agtcgaactg gctgaagata aactaaaagt tgaattttcg cctaatattt atctgacatt
      421 gaccgatcat cttcattatg cgatcacacg agcaaaagaa aatatccaac tgcctaatcc
      481 attattattt gaaacaaaaa aattttatcc gaaagaatat caagcagcga aagaggctct
      541 tttgttcgtg aaacaaaaga cgggtgtgga actttctgag gatgaagccg gttttattgc
      601 ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg caagtgacga tgaccgctac
      661 cacaatggta agagatatac tgagtatcat cagtaaattc ttcggcatcg tgttcgatga
      721 agaatctttg aattatcagc gtatcattac tcatctgcag ttttttgccc agcgttatct
      781 caaaggggaa ggatctgacg agcaagatga atttctctac gcattagttc aaggaaagta
      841 tccaaaagcg tttcattgtg tcgaacggat caatgagtat cttcgacaaa cgcaaaaaca
      901 ggaaatgggg atcgcagagc agatttattt aacgattcat attcaaagag tagtaagtga
      961 gaaaaaaagc atccaatcat aaggattgtt actgtatggc aggcaaaacc tggattgaaa
     1021 aaataggttc acgaatgttt gtgactgttt ttttcaatct gggtttttat tttttaaata
     1081 aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt ggtcgtcgtg tatgggaagc
     1141 ggtaggagga caaaagaatg tcaaaagttt agtacattgt gctacgcggt tgcgattcag
     1201 attaaaagat gaatctttag cagatacgca aaagttaaaa gaagacccgg acgtgatcca
     1261 agtcgtacaa agtggcggtc agtatcaagt agtgattggc agtaatgtag cagatgttta
     1321 tcaatcgatc gtggatgagg aaggtcttgc agtttcggag gatggaaaag aagagtccaa
     1381 aaatattttg aatcgtctga tcgatattat ctctagtata tttacacctt ttctaggggc
     1441 gatggcagcc gccggggtat tgaaaggatt cttatcattg gcaacggtga tgggctggct
     1501 gacagctgat agcggtactt atcaaatatt gtttgctgca gcagatggtg tctttacctt
     1561 cttacctgtc atgcttgctt ttacagcagc taaaaagttc aaagccaacc agtttttagc
     1621 agtagcaatt gctatggcac ttgtgtatcc agctatcaca gcagttgctg gtgcgggaga
     1681 agctatcagt ttctttggga tcccagtgat tctttcacca tctggttata cttcttctgt
     1741 cataccaatc attctagccg tatgggttca aagtaaatta gagccattcg taaaaaaagt
     1801 gattccgcaa tttctgcaaa tgattttagt tccgcttgtc gttttagtag tgatggttcc
     1861 attaacattt cttgctttag gaccaatcgg gacggttgcc ggtaatgctt taggcggtct
     1921 cttcaactcg atttatggat ttagtccaat cgtagcaggt ctaatcatgg gaagtttatg
     1981 gcaagtattc gtgatgttcg gcatgcattg gggattcgta ccgatcatgt ttttgaatat
     2041 cgaacaatat gggtttgatg tattgatgcc aatgcttctt ccagccatct tggcacaagg
     2101 tggggctgct ttagctgtag cattgcgaac aaaagatacg aaacttcgag cattagggat
     2161 ttcttctaca gtgacttcgt tatttggaat cactgaacca actgtgtatg gtgtgacatt
     2221 acctttgaag aaaccattca ttgccgcttg tatttctgga ggtattggtg gagctattat
     2281 cggattttcc ggagtaaaag ctttctccag cagtttggtc agcttattga cgattccgac
     2341 tttcattaat acggtggatg gtgtagagtc aaatgtgaca gtagcagtaa tagctacagg
     2401 aattgctttt gttcttgcat tcgtaggtac attgatttta ggatttgacg aacaaacaca
     2461 agacaatcaa ttagaaaaca aacatgcgaa tgcaggagaa ccgattactt cagcacggca
     2521 taccttaaaa agtcctttaa cgggtaaagt actgccattg agtgaagtac cagaccaagt
     2581 tttttcttct ggcgtgatgg gaaaaggtat cgctattgat ccagaagtag gagaactagt
     2641 tgcacctgca gacggagaga tcacgactat ttttccaact ggacatgctg tagggatcac
     2701 aacgactgac ggagcggaga ttttgattca tatcgggatg gatacagtag aattaaatgg
     2761 gaatggcttt gagatcttag tcaaacaagg ggatctcgtc aaagcaggag atttattgat
     2821 tcgttttgac atcgaagcaa ttagagcagc tggctatagc gtaatcactc cagttgtcat
     2881 tacgaatact gatgcatttg ccgatatact tgaattagac caaaaagaga ttattgcaaa
     2941 cgaagatgtt ctggcaattg taaaataagg aggaaatcag gatgcaatca cgtttttcag
     3001 aaaaattttt atggggtgga gcaactgctg ccaaatcaat tagaaggggc ttatgatcga
     3061 gacggaaaag gactatccgt tgcagatgct atgcctggag gtaagcagcg ctttgcgatt
     3121 atcggcagtg aagaatttga ctggacgatt gatcaagaaa aatatattta cccgaatcat
     3181 cgaggaatcg atcattacga tcgtttcaaa gaagatcttg ccttgtttgc ggaaatggga
     3241 ttcaaatgtt atcgtttttc gattgcctgg acacgaattt ttccaaatgg agacgaaggt
     3301 actcctaacg aagcgggatt agaattttat gatcagctga ttgatgagtg cttaaaatat
     3361 gatattgaac ctgtgatcac gatttctcat tacgaaatgc ctcttcattt ggcaaaagaa
     3421 tacggtggct ggaaaaatcg aaaactgatc gacttctatg aacgatttgc tcagacagta
     3481 ttagagagat acagcagtaa agtcaaatat tggatgactt tcaatgaaat caattcggcc
     3541 tttcactttc cagctttaag tcaaggatta gtaaaaagca atggagcagg agagtaccaa
     3601 aatattttcc aagcatggca taatcaattc gttgcaagta gcaaagccgt aaaaatcggc
     3661 cacgaattgc gttcggatat ccaaatcggc tgcatgatta tctatgcaac aacttacagc
     3721 atcgatgcca atccagtcaa tcaggcagca acaatgatcc aaaaccaaga attcaatttt
     3781 ttctgcacgg atgtccaagt acgtggagaa taccctgcat acacagcgcg tacccataaa
     3841 aaatatggag tcgatccgga aaaattagag caaacggaaa aagacttcca gttgctaaaa
     3901 gaatatccag tggactacat cggatttagt tattatatgt ctacggcaat caatgagact
     3961 gatccacaag cagcaacttc agaagggaac ttgctgggag gagtaaaaaa tccgtttctt
     4021 gaggcaagtg aatggggctg gcagatcgat ccagaagggc tgagaatcgc attgaatgaa
     4081 ttatacaatc gttaccagaa accattattc attgttgaaa acggtcttgg agccattgat
     4141 catatggaag aggatggcac gattattgat gattatcgga tcgattattt acgtcgtcat
     4201 atcgaagcga tggctaatgc agtagcagac ggtgtagatt tgatgggcta tacgccttgg
     4261 ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga gtaaacgtta cggctttatc
     4321 tatgtggatc tagacgatga aggaaatggg acactgaatc gatcaaagaa aaaatctttt
     4381 gattggtata aagaagtaat tgcctcaaat ggcgtaaact tgtaattaaa gcagctgaat
     4441 gaaaaagaac acacttctca catgtgattt gtgttctttt tttatttttt atgtgctatt
     4501 cttttagtaa tagtaaagca aagggtgacg ttatgaaaag gacacgtgta ctatacttgg
     4561 aagtcgcaga taaaattaaa gaagatattt tctcaggaaa atatccagtc ggttcgatgt
     4621 tgccgacaga aagtgaatta gaagaactgt ttcaagtcag caaaatcact gtccgaaagg
     4681 caatcgaact gctggcatca gatgaatatg tcgcaaagaa aagtggtcgt gggacgacgg
     4741 ttctaagcaa tcgtccttac aatcgattat ccaaagcggc aagctttacg caaatcttgg
     4801 agaactcccg atatgaagtc cggaaagaaa cgctggattt gcaattggtc catataaaga
     4861 aggaagatcc tttgttttcc tttttcggtg agcaagctgt ttgttttaag cgcttatatt
     4921 atttgaacaa tcagccttat atttattttg ttcattatct tccacaagaa atgatgcaat
     4981 tcagcagaga agaatttggc aaagaatctt tgtatcgttt gctgacgaaa aaaggatata
     5041 ctattgactc attttctgat gatttctcgg cagtattttt aacagaagaa gaacaaggaa
     5101 ttctgaaaac agacgcacag atcggcatca aacggatacg gaaatcgtct acgcaaattg
     5161 gggaaatgat tgaatattcg gaggcggtct accatacgga gctgcatcct taccatattg
     5221 attatgaaac ataaataaaa aaagaggttg tgaaaaagct gccctgcatc aagtaataag
     5281 aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttaggcttat taccgcagat
     5341 acacggctgt ttctggatta ccatttattc tggataaggg ggtgtggcac gattttcaga
     5401 aggaatctat gaaccaatca ctgtattgat tttagcttcg atggtaaccc cattcggctt
     5461 gacgatcgct tatttcttag ggaaaatcat tcgtaaaaat atactcaatc gtcaagaaat
     5521 cgatacattg aagaccgctt ttcctatggg gatctgtcag atcacagagg gatgtttccc
     5581 tatcgtattg aacgacttgg cacgcaatgt catagcaacg ggagtcggtg ggacagtagg
     5641 tggcggatta agcatgtttt ggggcgcaga tagccgtatt ccggcatcgg gaatgtttgc
     5701 agtcgctacg atgaccagac catgggcatt cattggtgca ttgttagctg gttcttttgt
     5761 cacaggaata accattttat tattgaaaaa accagtagat ccgaatgcag aaatcatcca
     5821 agaagaaaaa gaggaagaag acatatcttg ggatgatttg acaattagtt aggagaggca
     5881 gaatggatta caagaaaaaa gtacaataga tgtaccttga ggcaggaatg gtatgaaaga
     5941 atcagagtta gaaagaatcg aatatacaga ttttggttta ggagcgatcg aaactgtcca
     6001 gagcatcggc accctccttg aaatgacgat gaaggaaaaa tgagaacctt tcgttgtcga
     6061 aagggtctgg tataccttat gtcgaaggaa aggaaacaaa aaaactttcc tgtactattc
     6121 caaaaaggaa agaaagttat tatacagtaa aaaaagaaat tattcttcat gctagagatc
     6181 agtacacgtt cgagcccaat atcaaacact agttccaggc aggcgcaaca ggtgcggtta
     6241 tcactgaatt ttcttccagc agtgacgatt caagtgatat ttttacaaat ccagctattt
     6301 atcgttaaca aaaagatttt taggagaagc tgatgaacaa aaatgatcaa attttgatcg
     6361 tttagttcgt cggctttttt atttagagaa acatcattta tcagtgaaac ataacacgag
     6421 aacaaaatga ttttttcgtt aataaaagaa acaagtattt gttgtttaaa ataatacacg
     6481 ttattggcat ataggacgaa atcccagtat cttctataag taagtcattt catatcgatg
     6541 aaaacacgtt atttattttt gttttatcta taaaatggct gaaaaatgat ttttttctaa
     6601 atactatata tagtggcgtc tatcctatat accactaaaa aactacaaaa tctagtcttt
     6661 tattttaaaa aaacacgtgt agaatgaaga tagacttttg agttagggag tgttttttta
     6721 tgaaacttac tgttttttgc ggttcccgtt ttggtaataa agaaagttac aaatttattg
     6781 cgcaaacgtt aggaaaatac atggcacaag aagaaatcga actggtttac ggtggttcat
     6841 actccgggat tatgggagtg attagtaaaa cagtactgga aaataacgga aaagtcacag
     6901 gtatctatcc gaatgggctt tttgaaactg aattgcctgg aaaagatgtt actacattta
     6961 ttcctacaga aacgatcgat gaaagaaagg tattattatt tgaaaaggga gatgctgtcc
     7021 tcgttttccc tggaggtcta ggtacattgg aggaattctc acaacttctt tcttggatag
     7081 caatcggtct gacgcccgac aagccaatcg gaatattgaa tattggcggt tattacagcg
     7141 gcttgcaaca attattagaa acttttgcaa aagaggattt tatggataaa aaatggttgg
     7201 atcaagtaat cttttccaac aatccattgg aactggtcaa tgaattacga gcagtatcta
     7261 ttaaaaatca gttattattg aaggaggcaa aataatgagt caaatatatt tagcaggacc
     7321 atttttttca gaggagcaaa tcgatcgagt aagtcgtatc gaaaaagcgt tggaggaaaa
     7381 caaaacagtc acaagtttct actctccacg tcatcaccaa gaaagcaatt atgaactatt
     7441 cagcgctgga tgggcgcagg aagtatacga aaaagatatg gaagaactga caaatgcaga
     7501 atttgttgtt gctatccttg actttgagca tcaaacaatc gatccaggaa cggcttacga
     7561 attaggtgtg gcaactatgt tgaaaaaacc gatgatcatt gtacaagaag agacggtacc
     7621 tacgaacttg atgattacac aaagtctcca tacgtattta aaatcagacc aagctgtacg
     7681 tgaatatgat tttgaaacat tgcctgttga aacttacgta ggcgagtact tatagaagag
     7741 gaagggttgt atgtcagtga ttttgatttc aggaacgatt ggtgcaggta agagcagttt
     7801 gacggatatg cttgcaaaag agattgattc taaaccattt tatgaaaatg tcgaagataa
     7861 cgaagtattg ccgctctttt attcaaatcc tgagcagtac gcttttttat tgcaaatctt
     7921 ctttttgaac aagcgatttt tagcaatgaa aaatgcttta gtgaacgatg acaatgtgct
     7981 agaccgatcc atctatgaag atagcctgtt gttccatcta aacgctgatt taggtcgggt
     8041 aacggatatc gaagtacagc agtacgatag cttattagat acgatgctaa acgagttaga
     8101 tgatgttgct ccaaaaaaac ggcctgattt gatggtccat atcaaagttt ctttggacac
     8161 gatgctagaa cgtatcaaaa aacgtgggcg tgactatgaa cagctagaaa gcgatgagac
     8221 gttatatacc tattatgaaa cgttgaacac ccgctataat caatggtatg aagattttga
     8281 tgtatgtcct aaaatacaag tggatgggga taaattcgat tttgtagaaa atccagaaga
     8341 tgcaaaaaag gtgatccgtc agatccaaga aaaattagct gaattggaag gaaagcctgc
     8401 tggaaaatat tttacggata accagcatgc aaaaggtcaa gaattaggct aaaaataaaa
     8461 aaccaaacga cgggaaagaa aaattactac gtgatgtaga ataagccttt ctatcgtttg
     8521 gttattttat attcaaatat ttgaaataaa taaatcttta ctctttgatt tgtaaactga
     8581 aatgatccac tgtttgtttt actgatgaag cttgatcaaa ggaagaagct gagtttgaag
     8641 ttcataaaaa tcagccatag tgagttttgc ttggatttgt ttatcttctt ctacttttta
     8701 cataattttg aacaggataa gattcaaatg ttttaaatga atctttgact agtttgtgac
     8761 agttgtcgca tatgctcaag cttccttggt cattaaattc tgtatggata tttttgttgt
     8821 tattacatgt acaataatca ggactatcga tcatccattt cgtctccttt acaatcatct
     8881 tgcttcttta ctatagcaga aaagaagggg agaaacaaaa gaagatccaa cagacggcac
     8941 tatagactgt agatgttgcc atcctcatcc ttcaaacggt aattaccgat ttttgtaaat
     9001 atgactgttg tttctttttt attttctttt cctgaaataa tatattcgtc atctttggta
     9061 gtgatcttga tatctgtcag cactgttttt tcttcagatg attcagaaga atcagttcct
     9121 tgtgtctgat acgtgcgtgt ccattccatt tttttatctg taaatgtcaa ctgtcccgcg
     9181 gataaatcca tatctttacg tacttttagc gtatatacac tgttggtggt atctgctttt
     9241 tccgctttta agacagaaga atcggttgtt tcgtccgagg attggtcaga gccgccaaac
     9301 aaatcacagc cagtcaatgt caaaagtccc gcagacaaaa taaatagtac gattaatttc
     9361 ttcaaaatac ttcccccatt tgataggata aagaaattat accattggaa tgtaaaactt
     9421 caaacggatg gaagagaaaa acgcacaaaa cacccacaaa tagagtaaat tttagaataa
     9481 aattttaaaa aggcaaaaaa tccgccactt tcagaaagcg ttttaaaaaa gtgttgcaat
     9541 cttttaaaaa aatgctatat taaaaatgaa ccggttcaaa tacttgttta agtataatta
     9601 atagtgagag gaagttgctc tactgaagac tttagttata tggatattga tgacaagcaa
     9661 cactatgcaa agaatgcggt catcataatc gaacaacaag aaagagaaat caaatgtaac
     9721 tggcaggagt gagaatgtat ggcatcgatc cgtgatgtgg ctaagatggc tggtgtttca
     9781 gtgggaacag tttcccgtta cttgaatggt cagcaactga aggaaaagaa catgaaaaaa
     9841 atagcagaag cgatttcagc gttggactat aaagaaaaca tcattgctaa agggttgaag
     9901 aataatcgaa gcttctctat tggacttttg ataaacggga tttccagccg ttttgggtcg
     9961 gaagttgtct cagggatcga aagagtagct gaagaaaatg ggtatagctt attattaagc
    10021 gggtttgccg atcagccaga aaaaattgaa caaaaaattg aatacctgat gaagcatgtg
    10081 atcgatgggc tgattgtctt cttatccgaa gaagagtggt ctggatttga aatgctcaca
    10141 aaaatgagta ttccagttat cgcattgaat tgtccagaag gaccagcagg tattgataca
    10201 atattagtga atgatcgaga aagtgtagca aaagtgatcg ctcatcatgg tgaacaagga
    10261 cataaaaaaa tcggatttat tgctgccact caaacagact acgtagcaag agaacgttta
    10321 gcaggagcca aagaagcggt ccaaaatgat ccatcgatcc aattagaggt ctttacagga
    10381 gactattcta gaaaaagcgg ctattacggg gcaaaagcat tattagaaaa aggggcaact
    10441 gctatttttg tcagcaatta caatatgtcg attggtgcca tcgaatgttt caatcaagaa
    10501 ggtgtcagaa tcgggaagga tattgcgttc agtcactatg attatttaga taaaaacgat
    10561 ctgagtatcc ttccgaaaat cacgatcacg ccaccaacag ataaaatcgg ggaacgagca
    10621 gcagaacagc tgttaaaacg aatcaaaaaa gaagaggaag aaaatgaaaa agtgattgtg
    10681 atggaaaata tcatacatgg aatcaactaa aacgaactaa aaaagaaaaa gaggtgcttt
    10741 gatgaaaaaa tatcaatttc cagcaaattt ttggtggggt tctgcagcaa gtggcccaca
    10801 aacagaagga aggatttcag gcgatggaaa aggagagaac atttttgatt attggtacaa
    10861 aaaagaaccg caaaagttct ttgatcaagt aggacctgaa aaaacctcgc aagtttatac
    10921 gaagtatcaa gaggatgtac agttgatgaa acagactggg cataattcat ttcgaacatc
    10981 tatccaatgg agccgtttga tacctgatga atctggaaca atcaacgaag aagccgttcg
    11041 tttctatcat cgttattttg atgagttgat tgaaaacggt atcgaaccct ttgtgaatct
    11101 gtatcatttc gatatgccga tgtatctgca agaaaaaggc ggatggttga accgagaaac
    11161 ggttgaagcc tatgaaaaat atgccaaaag ttgttttgaa ttgtttggtt cgaaagtaaa
    11221 aaaatggttt actcataatg aaccaatcgt tccagtcgaa gcaggttatt tatatcgatg
    11281 gcattatcca gaagagtcag atatgaaaaa agcgatccaa gttggctatc atgaagcgtt
    11341 ggcttcagcg ttagccatca aatcttacca tgaaatggag caaggtggtg ttattggtat
    11401 catcttgaat ctgacaccaa gctatccaag agacgaatca aaccctgaag atgttaaagc
    11461 agcgcaaata gcagatgcgt tcttcaatcg ttcattcctt gatccagctg taaaaggcga
    11521 gtttccaagt gaattgattg agatagtaaa agaacttgat ctcatgcctg tgatccatgc
    11581 agaggatttg acggttatca aagaaaatac agtagatctt ttaggtatca attattacca
    11641 gccacgccgg gtaaaagcaa aagaaacacc aatcgatact aaaaacggtc caatgcctga
    11701 ggattatttc gacaattatg aaatgcctgg tcgtaaaatg aatccttatc gcggatggga
    11761 gatctatgaa caaggaatct atgatatttt gatcaatgta cgagatcatt atggaaatat
    11821 cgactgcttt atttctgaaa acggtatggg tgtagaaaac gaagcacgat ttatcaaaga
    11881 agatggcatg atccacgatg attatcggat cgaatttgtc caatctcatc tgaaatatgt
    11941 ccatcaagcc attcaagaag gtgccaattg tttaggctat catatgtgga catgtatgga
    12001 taactggtcg tggaccaatg catataagaa tcgatatggc tttatttcag tagacttagc
    12061 acaagatgga aaaagaacaa tcaaaaaatc gggtcattgg ttcaaagacg tatcagataa
    12121 aaacggattt gaggcttaag aaagaatagt tgagagcaaa tgcgtgaaat tctccattct
    12181 taccattttg tgaaaaagaa gttcaattat ttacgatctt tttcaacttt tttgtctttc
    12241 tatttcattt tttgtatata atagaatagt caaaattaaa taggagtgga ggaaagcatg
    12301 aggaaaaaaa gaccgtttct tctcttttgg ttattcggtt ttttcttact gcttatttta
    12361 tccgcagcat tttcaggaaa aatgacagca gatcttgctc agaaagaaac agaaaaaatc
    12421 gtagcaagca aagaatcagt agataatttc aaaaaagaat tctcaactta ttggtgggcg
    12481 gagtataaaa taactgccaa ttacgaaaaa ggcgaattgg acatttattt gccaaaagga
    12541 aaagaagaaa atcaatttat taatatcgat gacgtactta gtgcgatttc tctcagacaa
    12601 tatcaattta aagacaagca tttgaccgtt ttgcttttga ctcatgatgg cgacaaagta
    12661 gcccgtttga aaaatggaaa agtgatcacc gcttattctg aaaaagtaga caaaaaagtg
    12721 gtcgccgatc gtttagcgga atggaaaaag acaaatgaac aagtcaagaa aacaactgtc
    12781 tcttctcaaa aaaacgagtc gacaggaaca agtccttcag attctggcag tggacaaacg
    12841 ataacgggaa gtgaagaaaa tcaagcctca attattacga atgagtcatc acaaggacaa
    12901 tatcaatcct atggacaatg aaaaacagag gttgtgaaat cagcgttttg acctgagtat
    12961 tagagaaaca aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa
    13021 ggtcagctga ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac
    13081 agtcccttcg tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta
    13141 tttcttctat tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt
    13201 tcttgcccct ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac
    13261 ggctctttca ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt
    13321 cgcccaactg atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg
    13381 cagaatactg ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac
    13441 agatgctgtt ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct
    13501 ttcgtatata taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc
    13561 atccaaaact aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt
    13621 tgcatctcct ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag
    13681 cccatttttc tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt
    13741 tcacataccg cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct
    13801 ccggcagtgc ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa
    13861 aatattcatc taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat
    13921 acaacggaat ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca
    13981 tttgcgttat ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta
    14041 gatcaccagt caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga
    14101 attgttctgt cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct
    14161 ttctttttgc cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag
    14221 aacagcagct gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc
    14281 tgctgtgata atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga
    14341 aaccggtttg ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg
    14401 agcaatttta tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg
    14461 accagctact tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc
    14521 aaattgcaat tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc
    14581 ttcaactact ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat
    14641 tgctactttt ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc
    14701 agccaattct tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc
    14761 ttgatctctt ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt
    14821 tttgatgatc tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac
    14881 cattccgtct gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa
    14941 aatcttttct gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac
    15001 catttcatcc cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat
    15061 tcctctggca tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac
    15121 aagttcttct gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg
    15181 gaaaacgatt ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac
    15241 aagatgacct ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca
    15301 gtttatttta tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc
    15361 gaactgcact ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca
    15421 atcggttctt ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca
    15481 aaaaagccac gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga
    15541 ctcttatcga tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt
    15601 ggtttttccc gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc
    15661 ccgcgatgag aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact
    15721 ccctgtttag cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca
    15781 taaagctctt tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa
    15841 aaagataagc catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact
    15901 ttaacttctt cataaggagt caattccaat gcttcataga tatgatacat atctggatgg
    15961 atcttttggg acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt
    16021 gaaaattctg aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc
    16081 acctcctaat aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct
    16141 cagcacttga ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat
    16201 gatcaaatta attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc
    16261 tcctgaaaat gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc
    16321 tactggtcga aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc
    16381 gatgatcact ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt
    16441 gtcgattcca aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta
    16501 ctatgaagta gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag
    16561 ttttgcttcc atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat
    16621 ggcctcagct aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt
    16681 ggaagaaaag aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt
    16741 attaggtcca gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg
    16801 tcaaaacaat atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt
    16861 cgctcacgag cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga
    16921 tgtcagcatg ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt
    16981 aaaagattat gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat
    17041 attgcgcgcg attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag
    17101 aatttttcat tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa
    17161 aaggacattc cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc
    17221 tttatgccat gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta
    17281 cgcgttttga gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt
    17341 ggcccggtga cttttattat attcgtagac ttcactggac agtccaaggt gatatctata
    17401 atcatcaata tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac
    17461 ttttttcagg agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta
    17521 tttgtattgc tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc
    17581 ctgtttctta ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat
    17641 ttcaggtaca tctttttaga tattcttttg catatgatca taaagatgac cactgatcat
    17701 ccgctgaccg acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt
    17761 ttcatccaca cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc
    17821 ttctaataat cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc
    17881 caaataccat tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag
    17941 ctgatatttc tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt
    18001 ataaccgtaa gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata
    18061 actgtaacga taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc
    18121 ccctgtctgc ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc
    18181 gatttctgga gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca
    18241 ttctaacatg cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc
    18301 agtaatgaaa taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac
    18361 gactggcatt tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt
    18421 ttttccgcaa ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac
    18481 tctcttagct tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata
    18541 tccatacctg acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat
    18601 ctttgactag aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt
    18661 ccttcatcgc tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt
    18721 ctgcggcctt ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta
    18781 tgacttgtcc ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat
    18841 ctttttcaac tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga
    18901 ttgctgctgc ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa
    18961 aagcggcatt gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac
    19021 caaacatcat ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa
    19081 atgaacgaat gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc
    19141 gtgcagttct aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa
    19201 ggtatcttct ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc
    19261 ttcaatatcg tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg
    19321 tcgttgtggc gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc
    19381 taatttatag tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt
    19441 attaacataa aaatcacgtg atttctgata atctgaaaca atgatggcta tatgatggat
    19501 cgttgtaagg ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct
    19561 ccagttccat tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa
    19621 aaaaggcaaa aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat
    19681 aaatgaaagt aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat
    19741 tatttataat tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta
    19801 aatacagaac acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc
    19861 agtttttagc tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca
    19921 gtcttggctg ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta
    19981 catttatcgg taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag
    20041 tcaacggtat tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa
    20101 agaactattt aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat
    20161 tgttaagtaa agaatggaat cataatatgg cttcaaaaat ccgaaaattc actggaaaaa
    20221 gctggtttat cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg
    20281 gtcgttatac agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga
    20341 ctgctggtat catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag
    20401 ggcttgctca aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga
    20461 tgttcgtgaa cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt
    20521 ggtataacca aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac
    20581 aacaacacgc ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc
    20641 tctaaacacg tataaacggc ggaa
//
LOCUS       DACDYM010000045        19859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDYM010000045 DACDYM010000000
VERSION     DACDYM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.045"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            208..2250
                     /locus_tag="IWM59_001689"
     CDS             208..2250
                     /locus_tag="IWM59_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9979726.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="IWM59_001690"
     CDS             2261..2881
                     /locus_tag="IWM59_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9979727.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="IWM59_001691"
     CDS             2893..3351
                     /locus_tag="IWM59_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9979728.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="IWM59_001692"
     CDS             3370..3648
                     /locus_tag="IWM59_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9979729.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="IWM59_001693"
     CDS             3673..5040
                     /locus_tag="IWM59_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9979730.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="IWM59_001694"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="IWM59_001694"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP9979731.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="IWM59_001695"
     CDS             6227..6718
                     /locus_tag="IWM59_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979732.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7161..8360
                     /locus_tag="IWM59_001696"
     CDS             7161..8360
                     /locus_tag="IWM59_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9979733.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8855..9613)
                     /locus_tag="IWM59_001697"
     CDS             complement(8855..9613)
                     /locus_tag="IWM59_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9979734.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9781..10716
                     /gene="pfkB"
                     /locus_tag="IWM59_001698"
     CDS             9781..10716
                     /gene="pfkB"
                     /locus_tag="IWM59_001698"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9979735.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10713..12158
                     /locus_tag="IWM59_001699"
     CDS             10713..12158
                     /locus_tag="IWM59_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9979736.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12186..12641
                     /locus_tag="IWM59_001700"
     CDS             12186..12641
                     /locus_tag="IWM59_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9979737.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12660..13634
                     /gene="lacD"
                     /locus_tag="IWM59_001701"
     CDS             12660..13634
                     /gene="lacD"
                     /locus_tag="IWM59_001701"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9979738.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13745..14306)
                     /locus_tag="IWM59_001702"
                     /pseudo
     CDS             complement(13745..14306)
                     /locus_tag="IWM59_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14450..15064
                     /locus_tag="IWM59_001703"
     CDS             14450..15064
                     /locus_tag="IWM59_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9979739.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15305..15688
                     /locus_tag="IWM59_001704"
     CDS             15305..15688
                     /locus_tag="IWM59_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9979740.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16051..16560
                     /locus_tag="IWM59_001705"
     CDS             16051..16560
                     /locus_tag="IWM59_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9979741.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16660..17310
                     /locus_tag="IWM59_001706"
     CDS             16660..17310
                     /locus_tag="IWM59_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979742.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17761..18699
                     /locus_tag="IWM59_001707"
     CDS             17761..18699
                     /locus_tag="IWM59_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9979743.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18712..19764
                     /locus_tag="IWM59_001708"
     CDS             18712..19764
                     /locus_tag="IWM59_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9979744.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
BASE COUNT         6329 a         3150 c         4195 g         6185 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg attactcgat ccctgctttt tttaacttcc tttgcagcaa aaaaagctcg
     6901 taatctaaga gatgatggat agtttttaga atatatatat tttcttcaat tccagaaaaa
     6961 aatgaccaag ctagtatgct aaaaaagtac gatgcttcta tgatcttttt tccgtcttta
     7021 ttaacagatg ttttaattga tcaacgcata ttgtaatcta ccctaatttc catagttctt
     7081 caccactttt aaaaatagta aaacccctac aagtatgcta tagtaaattg ctaaagaaaa
     7141 accaataagg tgggagatgt gtgcgcgatc aaacaagcgg aaaaatgatt cgtttttatt
     7201 atacaagttt gcattttctt tattggatga tgttttgttc tgtctatggg tttgcgacat
     7261 tgtttttgat ttatttcaag attgatccag ctaaaatcgg agggatctta gcattggtca
     7321 atattgtttc aacggtgctt cagccttttg tcagtcaagt gatcatcaag aaaagaaaga
     7381 tcccactgat caccgtgctg aagacgatga tagcaggatt aggagtgatt ctcttagctg
     7441 gggtattgat tccgaatttg ccgatcatta cttatatctt gggtgggatt ttagtagttt
     7501 ctatgcaatc atttctcaat gcgctggcat ttgagtattc aaataccggc tacaggatca
     7561 actttggctt ttcaagagct ggtggttcat tttcttatgc tgtaacttct tttttactgg
     7621 gacagctgtt agcgatttat tctgccgcga ttttgccaat cttggttata gttgaagcag
     7681 tgatattttt cgttattttg catctgctgc cggatgtaaa aaatcaagaa gtttttccta
     7741 tgacaaaaga acaggatcgc tcagtttctt tgagaaaaaa atatcctttc ctgattttgt
     7801 tatttttagg tttcagtttt ctttttactt tccacaccat gatcaacagc tttttggcac
     7861 agatctttga aaatatccac ggcggcagta aagaagttgg catcgcattg atgatcgccg
     7921 ctttatgcga attaccggga atgatctttt ttgaacagct tgtcaaaaag aaatccagca
     7981 aattttggct gtctgttgcc agtatttcat ttgctgtcag aggattcatg atgctgctgg
     8041 ccagttcgat cctgatgatg gaaatgacgc atctcctaca agccttcagt ttcgctttgt
     8101 ttattccagc ttcggcttat ttgttcaatc aatttctggc ggatgatgat cgtgtatttg
     8161 gacaaacgat gatcatcatc gccacaactc ttggtggggt gataggaaat gtcttgggcg
     8221 gcagtctgct gcagcatttc ggtgtatggg agatgctgat tttcggtact ggttttgcgg
     8281 tgctaggtgc tttattaact attttgggca ttcgaaaaat accgaagaca ggaaacgata
     8341 gtgattctct tgttgagtag cataaaaata cttagatact agttttatat aaataacata
     8401 tagtgattat tggcgtaatt acaaaaaacg agaataactt aagtaagcat ttctaaataa
     8461 ttgagaaacc tcatctaaaa agataagaaa atatccctaa taaaacttct gtaacatttt
     8521 agataactga ttgtaatctg aataataaac tataaggaga cggttaggcg gtctcctttt
     8581 tttgcgtatt ataattctag aattgttgga aattgataat aattaacaag aaatattaga
     8641 caggaacttc gaactgctta tcacattctt attattatat atttgcgtaa gtagtcctgg
     8701 tagtctcaat gcactattct atcttgaaac taagaagtta tagaaggagc aatgatagaa
     8761 agcacgagaa tacaaatagc gattaaatat ttttctagac aaccttagtt aattcacaca
     8821 gatatatttt aggtacagtc ttacagatat agagttagtg ctgtgtgaat cctgatgttc
     8881 gcagtgcata ggagtcaagt aagattctag ccagtataga aagtggaagt ctggagcgta
     8941 cctcataatc tgggaagaag gagccttctg atttaaagcc aacaaaagga atctcgatat
     9001 gttccatcaa acttgaatga cgattagcag taagcaaaat tgtcccgatc tcagctttat
     9061 aacagttttc agctgcggta gtcaattcct ttgtttcacc gtttagtgaa acaaagatca
     9121 cgatgtcatc tttggataat tttctactaa tgttttttat gatattggga tcagtatgca
     9181 tctcacaata tttaccggta agttgaaatt tgaccatcat ttcctgagca atcaattctg
     9241 aaaaacctcg ggcaaaaatc atgattcttc tagcagcctt gattcgttga atagcatctt
     9301 caatattccc gctttctacc atattgatgg tgcggattac ctcttgctca tttttaagaa
     9361 tagcagtctt tattccttca tcaactttat caagagcggc aaaattgata tttgtatttt
     9421 ctttttcttt taacgaatgt ttaaaagacg taaacccctc atatcctttt ttcttcatag
     9481 tacgaacgac ggttgctgtt gaaacgttgg catattcact tagttttaca atggaatagg
     9541 aggggatttc gagtatgtga ctttgaataa aatcccagag atatttctcc gattcactta
     9601 attttgtatc catattttgc ctccgattag ttaggataat tctattgtaa agcgggagtt
     9661 tcctttactt caagtttatt taatatattt tgtaaaagtt ttcaatgaat ttagtcattt
     9721 atgaaaacat ttacattcga tcttgataca taatgaatat gtaagaaagg aggagcgtaa
     9781 atgtcagttt atacatgtac tttaaacttg gcaatcgacc tttttatcga aacagatgaa
     9841 atgaaaccat cccaagtcaa tcgaacaaac gatgatgata tacaagctaa tggaaaaggt
     9901 gtgaatgttt cattgatcat gaagcaatta ggtatcgata gtacagcttt aggatttaaa
     9961 gcaggtttta ccgggaatta tattgaggat tatttgcatc aaaaagatat tagcactgat
    10021 tttattagcg taccgggaat gactagaatc aacgttttta cacaagttaa taaaaccaat
    10081 gaagaattca aattggttaa taaaggacct gagattcctc aggaacaaat caaaactttt
    10141 ctaaatcaaa tacaaaagct gcaagctgac gattatttgt gtgtttctgg cagtttgccg
    10201 cgaggggtct cacccgaaat tcttattgat atcagtcgta tttgttttga aaataaggtc
    10261 catctgatta ttgattcaag ctatccggaa ataattgatt gtctgccgta tcagccgttc
    10321 ttattaaaac caaacgagga ggaattgcaa tcttggtata atgtcgagct tagtgatcgt
    10381 gaaggatata ttcattatgc tgaacgcttg attgccgatg gagcgaaaaa tgttctcctt
    10441 tctctaggag gagagggagg aatattttta aataaggata tcgtttattc tgggaattct
    10501 cctaaaggta aagtagtcaa tacagcgtgt gctggtgaca ctttgttggg aacatttctt
    10561 gcaggatatt taaaaggtga acaggtagat attaatttga agaaaagttt agcagctgga
    10621 agttcaaccg cttttcgtaa aggattaaca gatttttcgg atgttgcaga gttggaaaaa
    10681 caaatcgaaa tcaaagaggg agcgagtaaa aaatgacaac ttacaaaatt gttgccgcaa
    10741 caggatgtcc aacaggaatt gcccacactt atatggctca agaagcattg gagcaggcag
    10801 ctaagcgcaa aggggtatct atcaaagttg aaacccatgg tcaagtaggg atagagaatg
    10861 aattaacacc agaagaaata aaaaacgctg acgctgtaat cattgcagct gacaaagatg
    10921 ttcaagcaga acgtttcgca ggtaagcgtg taatcgatgt gcctgtcagc aaggggatta
    10981 aagatgctga ccaattgatc gaagatgcgt tggctggtaa aggttcaatc actgcagaaa
    11041 acaaaacagt tgacgctgct ggtgagaaag cggagatggg ggttgcagca ggcattggtc
    11101 acagtattta taaaaatctc atgaacggtg tttctcatat gctgccgttt gtagtggcag
    11161 gtggggtaat tattgcaatt tcgtttgcca tctggggtgt gtactctttt gatcccaatg
    11221 atcctactta caatccgact gccgctatgt taaagggtgt tggtgatgcg gcaatgggaa
    11281 tgatggttcc tattttagca gcttatatcg ctgaaggaat cgctaaacgt cctggtttgg
    11341 ttgttgggtt tgttggcgga ctgttagcca attctggcgg agccggtttc ttaggcggaa
    11401 tttttgcagg tttcttagca ggctacttta tcttactatt gcaaaaggtc atgaaaggct
    11461 tgcctaaaca attggatggt ctgaaggcga tcttcttata tccggtgatc ggtgtcttgt
    11521 ttgttggtgt agtcatgtct ttattagcaa atccaatgga agcagtcaac caaggaatga
    11581 tgaatttctt ggcaggattc caaaattcta atccattaat tttagggatt atcgttggct
    11641 gtatgtcagc atttgatatg ggaggtccag tcaataaagc ggcttatgtt acagggactg
    11701 cgttgttggc acaaggtaac catacattta tggcaggggt ttcggcagct tgtatcgcgc
    11761 ccccattgat tactggattt gccgcactgt tttttggaaa atatttcgac acgaatgatc
    11821 gcaacgctgg tttagtcaac tttatcttgg gctcaacgca cattaccgaa ggagcaattc
    11881 catttgcggc taaagatccg ctgcgtgttc tgccaactat gatgctaggt tcatcaattg
    11941 ctgctgtact gacatatatg tttggtgtcc aagttcccgc acctcatgga ggttttctag
    12001 tattacctgt tgttactggt aaggttgcat gggtagggtc gattttactt ggttcaatcg
    12061 ttggcggagt aattttaggg ttgatccaaa aacgtagtgt agaaaaacgt gaagcagaag
    12121 cagctgctgt tgaaatgaaa gaagcaacca acaactaaag gttattctag aaagaaggat
    12181 ttcaaatgac tttgatcaaa acaaataaag tttttatgaa taaaaatttt acgacgcaag
    12241 atgaggtaat gaacttcctt gcagatcagt caattgtctt gagcattagt gaagaccgag
    12301 atgcagttta cgaaaaattg atggaaagag aagaagaaag cacgactggt atgatggatg
    12361 gttttgccat ccctcatgcc aaagatgcta gtatcaagga tgccgatatt gtgattgttc
    12421 ggttaaacgg taaggtcgat tggaaaagtt tagatggaga aggaacggat tttgtaatcg
    12481 ctctgtttat tccagattca gaggctggaa caacccatct aaaattttta tctcaggtag
    12541 ccagactttt gatgcacaaa gatgtgacag aaggtctgaa acaagcccag tctgctgaag
    12601 aaatagctga acttttaaac caaaaattag aagaaaacta aaaaggaagt agtgaaatca
    12661 tgcctttaac agaaaaaaag aaaacttata ttcgtcaact aagtaatgaa agtggcatca
    12721 tcagtgcgtt agcaatcgat caacgtggtg ccttgaaaaa aatgatcggt aaatttcaaa
    12781 ataaatcagc aaccgatgaa caaatcatca atttcaaaaa aatcatttct tctgaattga
    12841 ctccatatgc ttcagcaatc ttattagatc cagaatatgg gttgccggca gccgaagttc
    12901 gcgataaaaa tgctggtctg ttgttggcct atgaaaaaac tggttacgat gcttcaactc
    12961 caggacgttt accggatatt ttatctattt ggtctgtaaa acgtctaaag gagcaagggg
    13021 ccgatgcttg taagttcttg ttatactacg atgtagatga atctgatgac atcaatgacc
    13081 aaaagaaggc gtttatggag cggattggtt ctgaatgtgt tgctgaagat ttgccatttt
    13141 tcttagaatt ggtttcttat gatgccaacg atcctgatac ttcttcgaag gaatatgcct
    13201 tgaaaaaacc gcataaagta aatgaaatga tgaaggaatt ttctaagcct cgctatggag
    13261 ttgacgtttt gaaagtggaa gttcctgtta atatgaacta tgtggatggt tacactaaaa
    13321 atgaagttgc ttataccaaa gaagaagctg ctgcttattt caaagaacaa tccgagacta
    13381 ccaacctgcc atttattttc ttgagcgcag gagtaactgc tgaattgttc caagaaacct
    13441 tgaaatttgc aaaagaggct ggctctacat tcaatggggt actctgtggg cgcgcaactt
    13501 gggcgaatgg tgtagaacca ttcgttacta aaggtgaata tgctgccaaa gaatggatgc
    13561 agacacaagg acgcaagaac atcgaagagc tgaataatgt tttgaaagaa acagcaacag
    13621 ctattaaatt gtaagaaagc tttaactata accttaagtt tgtaacctaa tgatttttaa
    13681 taatcaattt gagcttttaa ataaagaatt ggaagcgtcc cgacgcttcc tttttcgttt
    13741 gtttttaagg catgataggt tctatgttgt cgttcaataa gcgcaagaag gtcagcattt
    13801 ttttcttaga gtatgcaaaa ttgcttttct acccaatgat ttaaaacgga gaggcaccct
    13861 tggatatggt agatggtgaa gtaacgcatt tcttctgtga tcacggtttt tttgtcttct
    13921 tgtagccaga tttttaaata attttcttcg atcagattat ttaatctctt ttgaaattgg
    13981 atatctccgt ggttgatgac gaataacttg tatacgaacg tattcgtata gacatagtcg
    14041 atcaattgag cgtatagatc ctcgtagcta tgtgtcgtat cgtaggaaat gatttcatcc
    14101 aagtcttgga aaaagctatt ttccagtttt tcataaagat catagatatc cgtataatgt
    14161 gtataaaatg tcgaacggtg aagatcagca gtttcggtca gttcacggat ggtgatgttt
    14221 tgaatttttt tatctactaa taattttgat aatgcttctg ctaacgcttt ttctgttttt
    14281 ttgacgcggc gatcctcttt tttcattttg cagcctccta atcaacataa tcaaggaatt
    14341 gtgtcggtta tccgacaatt tcaccgctat ctgaacatag ataaccgaca ccttgtctat
    14401 tataatataa gtatagcata tcaatattta aagaatagga gaataagtga tggaattagc
    14461 ctttaagcaa ctggaaaaga aagactacaa aaaagccatt caattcgcga ttaaaggtat
    14521 gcactttgat tggtatacca agaacaaact ccttttgaat ctttatggaa gatacttttg
    14581 gtatctagaa ttgaatcgcg cgacacaaat cattgcttta tatgcggatg atgagttggc
    14641 aggtgtttta ttagcagaca tcaaaggaaa atcaaaaaag catcattcat ttagtcaaaa
    14701 gttatatgtt aaactatttg atttcttaca aaatgcattt gtgaaagaca gcaaaggcgg
    14761 gtatgacgac accaatgacg aattgctttc agactattta aaaaaacatt ccccagatgg
    14821 agaaattgtc tttttagctg ccaaccctga tttaaagatc aaggggatcg gtagtaaatt
    14881 attaaaagaa tttgaacgcc gagagcaagg aaaagaagta tttctgttca cggatagcgg
    14941 ctgtacgtat caattttatg aacgacgcgg tttcgaacgg aaaaaagaaa aagataacgt
    15001 catgaagatc cttaataaag aagtagatct ccgctgtatg ttatatagta agatcattca
    15061 gtaatgtttt ttactatcag aaagcagaag taccacgaag gtcaatggaa aaagcgagta
    15121 attgatccat ttagatgaag ataggtgtag tctcaaagat attagctggc aaagatatcc
    15181 ccagttaaaa atagatttct gtatccaaca ataaaaatat ttttgtattt attagtatat
    15241 atactttaca aaaatttgta tatatactaa tttttttgta ggatcaatga aatgagggga
    15301 aaatatgcca aaatataaag aaatagcctt ggctctaaaa gagaggatcg ttgagggaaa
    15361 atatcaggaa ggttctaaat tacctgagca agaaagtctg gctagagagt ttaaaacaag
    15421 ccgtgttact atccgcaaag ctatacaact ccttattgat gaaggtttgt tatatacgag
    15481 acgtggatct ggtactttta tacgaagcaa tatcagacag gacaatgaga atgtcactca
    15541 agtaaataat gtatttggca cttcctcaca agaaaataca aatgtgacaa gtaaaataat
    15601 ccgttttgat gtccggtttc cgacagatta tgaaaggcgg ctttctgcaa cctacttccg
    15661 atccccttcc tgcaaatcat gggtataaaa aataggcata ttaagcgggc taggaggaga
    15721 aatattcaca tatcattttt tcattccgca agctgcgtca agttatttgg aatttgtaga
    15781 acagatgaga gaggtagaag cagcactaca aactactttt caataagtag gaattataga
    15841 aattttactc aaaaaatctt cattaaaaaa tctgactaaa taagttgaga aaacagaata
    15901 tacttcaata tccagtgatt tataaatatc attcactcat gatacccagc tccgtatcat
    15961 aagtgatgcg gtttttatca ttgatcctaa aaatcttagc aagacaaaaa gtacgtaaga
    16021 aagtaaagac taaatacttc ggaggaacaa atgaataaca atgataggtt attgcgattg
    16081 cgttatgcga ttgatttgaa agatacggat ttgattaaag cttttgagct aggcggtgta
    16141 ccactgacta aagaagaagc tcaggcggta ctgaccaaag tacaggataa gaacaaagat
    16201 aatgcagaaa ataatgtgta tgaaaaaacc atcaacaacc aagtttttga cgcattccta
    16261 aatggcctga ttctcttggc gcgtggtccg caaaaagata agccgatagt agacacgccg
    16321 caaaaaagca aggaaatcaa atatatcaac aatgtgttgc taaaaaaatt aaaaatcgcc
    16381 ttgtctatga ccactgatga tattttagaa gtttttgctg aggcagaact ctatccatca
    16441 aaaggcgaaa tcggggcatt tttgagaaaa gaaggccaac gaaattttaa gccttgcgga
    16501 gataaatata tgcgtaattt tctcaagggg ttaggaattt ataatcggag aaaagtataa
    16561 agaaatttca tgagaaatca aaacaatggt tgttttgatg agagctagtt tttgtatgag
    16621 attaaaatat tatcttaatc gaggagctgg attggtttta tgaatgatga atataagaat
    16681 gatgaagata agatgctttt tgaagagata gagaatcgat gtaggctcaa ttttgagctt
    16741 cggggaaaga tgtctttaat acaacaaaaa aagtatttag cgaataaatc agagtttact
    16801 ctgggacatg ttgaaaaatt aatctcggat tggattagta gtagaagcga atttacaaaa
    16861 ataaaacagc cgattaaatt tgacatgaaa aaacttttgt taaataagag tgagataggt
    16921 aatagagatc aatatattag agctaaaggg caagaaataa ttgattcatt aggtgaagtg
    16981 aggtcataca attatttgta tgtaacacat agagctgatg gaatggtcat tactgtgggg
    17041 aaatcttctt ctaatgatat ttttttagat ggagatcttt tttatcaact caatacaaat
    17101 cacttgtctg gcactgaaaa tataatttta aggacggaat atggcaatga aatatttgca
    17161 aaatatgatg agattttaaa aaattatttg gactgggctt ggataattcc ggttgagtca
    17221 ggagatgcta agaaactgga gagattattg ggcgatgaac taattaataa aaaagtacca
    17281 attttaaatt attattccca tagacaataa aaggctattt ttcatgataa tactagttat
    17341 aactgtaaaa atggaagaaa atctgggaaa gaagttttta aataagtgta ttgttcgatt
    17401 ttaataaata ataagcaata ccatccgtct gtgttgcgca acatcgtact acaatgaata
    17461 ttttttcaca aaaagaaaat catccatcga atgaatggaa ggttttagta tacacgaggt
    17521 taatgtcccc tcttttataa tcagaggagt gaggtttttt ctatgaatgt ttctattttc
    17581 ttattgctag tctagtttct cttacgatga aattgggatt ctattccagt ttttattgat
    17641 ttgctgataa aataacttga atggtggata taaacatgat aaataagaga agaataacaa
    17701 aacaaggttg tgaaatgatt ctcaaataag tataatagaa agaaaaagga ggaagaatca
    17761 atgaaaatag caattgcagg cgctggcgca atgggaagtc gctttgggtt aatgcttcac
    17821 caaagtggga acgaagtatt attaatcgat ggctgggcag aacatgtcca acaaatcaaa
    17881 gaacatggat tacaagcaaa ttttaatggt aaagaggtag aagcaaaact accaatcgtt
    17941 cttcaatccg aagtagaaaa agaagatcaa gttgatctga ttattctatt taccaaagcg
    18001 atgcagctgg aaaaaatgct gcaggatatc caatcattaa tcaaaaaaga tacagaggtc
    18061 ttatgtctat taaatggtat cgggcatgaa gatattattg agaaatttgt accaatggaa
    18121 aatatctata ttggaaatac catgtggacg gcaggtcttg aaggtcctgg tcaggtcaaa
    18181 ttatttggaa gtggttcagt agaattacaa aatttaggtg atggaaaaga agcagctgca
    18241 aaaaaattag cagacaaatt gtctgaatca ggattaaatg ctcatttttc tgacaatatt
    18301 cactattcta tttatcgcaa agcatgtgtg aacggaacaa tgaacggact atgcacaatc
    18361 ttagacgtta atatggctga gctaggaaaa acatcgactg ctcataaaat ggtggcgacg
    18421 attgtcaatg agtttgccaa agtagcagca gtagagaaga ttgaactaga tgtcccagaa
    18481 gtcattgcac attgtgaatc ttgttttgac ccggaaacaa ttgggttaca ttatccttca
    18541 atgtatcaag acttgattaa gaaccatcga ttaacagaaa tcgattatat caatggcgca
    18601 atttctagaa aagggaagaa atatggtgtt gcgacgcctt attgtgattt cttgactgag
    18661 cttgtccatg caaaagaaga tagtttgaac gtaaaataaa ggagagattg aatggaaact
    18721 tcacaaaaaa tatcaccgaa aatttttctg aataaagtcc tcgctggtac agcaacagga
    18781 atcattattg gattgattcc taatgcagta ttaggtgcta ttttaaaata ttttagtgcg
    18841 tatccctttg cggtaacgat cgcacagtta gcagttattt ttcaattagc aacacctttg
    18901 atcattggtg gtttgattgc cttacaattt ggatttgatc cgatgagaat gatggttgta
    18961 gccggagctt cttttgttgg ttctggtgta gtcaaattca cacctgattt gggtgaaacg
    19021 ggtgtttacg ttggtgctgg tacaggcgat attattaata cgatgctgac agcatcgatt
    19081 gctgtcgggc tgattttact gattggaaat agttttgggt cagtgtcgat tgtgttaaca
    19141 ccgattgttg taggtgtagg tgcaggttta cttggttttt atttattccc ttttgtaaca
    19201 gcaattactt ctgcaattgg atcattgatt aataacttca ctaccttaca accgctgtta
    19261 atgtctatct tgattggttg ttcctttgcc tttttgatta tctctcctat ttcaacagta
    19321 gcaatcggaa tggcaatcca actagatggt gtttctgctg gtgcggctgc gatgggcgta
    19381 gctgcaacca cttttgtttt agtagtgaac tcttggaagg ttaataagtc aggtgtgaca
    19441 attgcgattg ctttaggcgc tatgaagatg atgatgccta atctatttag aaaacctgtg
    19501 attcttattc cttgtttagt tagtgcagtg attacagcta ttcctgtcgc tctattttct
    19561 atttcaggta cgcctgcttc tgctggtttc ggtgtggtcg gacttgttgg tccgttggct
    19621 tctttagatg cagggctaaa tggctttctt gttgttcttt gttggttagt catccctgtt
    19681 tttgcggcac taattactca atttttatgc gagaaagtat ttaaactgta tgatcgagaa
    19741 gaagtcttca aatttttagg ctagtagatt ttactaagaa attttcaatc aatggatgcg
    19801 taagtgagat gtcaacaaca tctgacttac gtttttttgc caaaatatgg tttttattt
//
LOCUS       DACDYM010000046        19255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDYM010000046 DACDYM010000000
VERSION     DACDYM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.046"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..575
                     /locus_tag="IWM59_001709"
     CDS             <1..575
                     /locus_tag="IWM59_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9979745.1"
                     /translation="IGVFLLITSPLYLTGAYRLDSGNANIVPLITSFILFIAVGASEE
                     LLFRGFFQHQMLRFGPLIAMIGSAALFALLHGLNPNMTFLAVFNIFLAGCFFSALIYS
                     TGSLFTAIGAHITWNWTQGAILGIPVSGTQEQGFFSTLIQSQNTIITGGNFGAEASIS
                     TTLFLLVLIVGLLLYAYKTKRMKKYTAVYQ"
     gene            771..1691
                     /gene="sgrA"
                     /locus_tag="IWM59_001710"
     CDS             771..1691
                     /gene="sgrA"
                     /locus_tag="IWM59_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9979746.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVN
                     NELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSR
                     TLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            1989..2345
                     /locus_tag="IWM59_001711"
     CDS             1989..2345
                     /locus_tag="IWM59_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979747.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            2348..3505
                     /locus_tag="IWM59_001712"
     CDS             2348..3505
                     /locus_tag="IWM59_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979748.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(3531..3971)
                     /locus_tag="IWM59_001713"
     CDS             complement(3531..3971)
                     /locus_tag="IWM59_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9979749.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            4131..4637
                     /locus_tag="IWM59_001714"
     CDS             4131..4637
                     /locus_tag="IWM59_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9979750.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4805..6717
                     /locus_tag="IWM59_001715"
                     /pseudo
     CDS             4805..6717
                     /locus_tag="IWM59_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
     gene            6722..7723
                     /locus_tag="IWM59_001716"
     CDS             6722..7723
                     /locus_tag="IWM59_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9979751.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(7925..8131)
                     /locus_tag="IWM59_001717"
     CDS             complement(7925..8131)
                     /locus_tag="IWM59_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9979752.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(8777..9625)
                     /locus_tag="IWM59_001718"
     CDS             complement(8777..9625)
                     /locus_tag="IWM59_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9979753.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            9789..10691
                     /gene="gnd"
                     /locus_tag="IWM59_001719"
     CDS             9789..10691
                     /gene="gnd"
                     /locus_tag="IWM59_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9979754.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            10693..11694
                     /locus_tag="IWM59_001720"
     CDS             10693..11694
                     /locus_tag="IWM59_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9979755.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            11691..11975
                     /locus_tag="IWM59_001721"
     CDS             11691..11975
                     /locus_tag="IWM59_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9979756.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            12008..12805
                     /locus_tag="IWM59_001722"
     CDS             12008..12805
                     /locus_tag="IWM59_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9979757.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            12819..13652
                     /locus_tag="IWM59_001723"
     CDS             12819..13652
                     /locus_tag="IWM59_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9979758.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            13670..14527
                     /locus_tag="IWM59_001724"
     CDS             13670..14527
                     /locus_tag="IWM59_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9979759.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            14549..15058
                     /locus_tag="IWM59_001725"
     CDS             14549..15058
                     /locus_tag="IWM59_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9979760.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            15095..15496
                     /locus_tag="IWM59_001726"
     CDS             15095..15496
                     /locus_tag="IWM59_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979761.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            15608..15961
                     /locus_tag="IWM59_001727"
     CDS             15608..15961
                     /locus_tag="IWM59_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979762.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            15995..16438
                     /locus_tag="IWM59_001728"
     CDS             15995..16438
                     /locus_tag="IWM59_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9979763.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            16455..17540
                     /gene="uxuA"
                     /locus_tag="IWM59_001729"
     CDS             16455..17540
                     /gene="uxuA"
                     /locus_tag="IWM59_001729"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9979764.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            17533..18246
                     /locus_tag="IWM59_001730"
     CDS             17533..18246
                     /locus_tag="IWM59_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9979765.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            18998..19198
                     /locus_tag="IWM59_001731"
     CDS             18998..19198
                     /locus_tag="IWM59_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9979766.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         6681 a         2982 c         4037 g         5555 t
ORIGIN      
        1 gaattggagt gtttctgctt attactagtc cattatattt gacgggggcg tatcggttag
       61 attcaggaaa cgcaaacatt gtgccgctga taacgagttt tattttattt attgcagtgg
      121 gggcatcaga agagctttta ttccgtggct tttttcagca tcagatgctt agatttggtc
      181 cgttaatagc aatgattgga tctgctgcat tatttgctct tttgcacgga ttgaatccta
      241 acatgacatt tctggcagtt ttcaatatct ttttagcagg ttgctttttc agcgcactta
      301 tctacagtac aggaagttta tttacggcta ttggtgctca tattacttgg aattggacac
      361 aaggagctat tttgggtata ccagtcagcg gaacgcaaga acaaggtttt ttctccactc
      421 tgatccaatc gcaaaataca ataattactg ggggaaactt tggggctgaa gcatctatat
      481 caactacatt attcttgtta gtcttgattg ttggtttgtt actctatgca tataaaacaa
      541 agcggatgaa gaaatacact gctgtttatc aataaaagat gaagaaagca aggctgatat
      601 cggtcttgct tttttaagtc tgtcattgat taaacgaaaa agtaaacaaa ttcaagcgaa
      661 tacacgacat cttttgttag attttcacaa ctgactgtcg tgttctcatc attttcattt
      721 tatataattc ctttatcaaa tagaaggtaa ttatatggag agtgatagaa atgaagaaaa
      781 cagcgaccgt attatttgtt attcttgggc tgagtataat tgcagcaatg attggaatca
      841 gtacttctgc aaatgaacgg gcaaatgaga atctttcatt tactgtgaaa actgatcgga
      901 ttgtttatga catgacacaa caagtcatta ctattccagt caaaccaaac aaaagcgtga
      961 atgctagtga tgtacatgct gttctaacat atggttggga tggtaatgga agttcagaaa
     1021 aagttattgg ggaagtttat ttaaaagacg tacaatggac cgctggtatt gagtatacaa
     1081 taatgatttc tgcagaacta tcgattgatg agatcaagag taaagataaa gttgatttga
     1141 ttgtttttta tgatggacag atgacgatca cggagaattt gaaaccaagc agttggacag
     1201 ttgttggacc gccaagcagt acggatagca caagtagtga aagcagtaca gaaaacacga
     1261 gcggtgaaag cagtacagag agtacgagca gtgaaagtag cacagaaaac acgagcagcg
     1321 aaagcagtac ggagagtacg agtagtgaaa gtagcacagg aagcatgagt agtgagagta
     1381 gcacaggaag cacgagcagc gaaagcagca caggaaaagt aaataatgaa cttgatttgg
     1441 tacctatcat accaactaag gaacaaaaga gaaatgcagg gagcaaccta aaaagtgtgc
     1501 taaaacagcc tgattttgtt tctcgtggaa ttgatcatca agaaaaagcg aaagaaacag
     1561 ataatagaaa aagcgattta ccagaaactg gtagtagaac cttgaacaag ctaattacat
     1621 ggatgggtgt gttactcatt ttgattgtag gagcaagcta ttttcgttct ctcttccatc
     1681 gagtgaaata aagaataaaa gcaacagatc taaaattgaa taaaacgata gatttttgag
     1741 tgaatatttc attcgagagt gctgtattgc ctggcgaagt tgtagctttg ccagatctat
     1801 ttttgtttac taacatattt gagaaacagg aaataacagg aacacaaatg gaaaataaga
     1861 gcaagaataa ttgacaatgc acactgcgca tggtagtatc tctgaaaaat cagtaacgaa
     1921 tctatttgta cgttattcat gtagctgtcg gaataagtct aaataataga taaaaaggag
     1981 tatgtacttt gtttgaagaa tttattgata tcaatgaaag gcaagtatat caatttttga
     2041 actattgtta tgaaagagac gagaaattat atgtggtgaa ggatatcgct ttagatctaa
     2101 actacaccct ggctaagatg aattcggtga ttcaacaagc agaatcgttt tgcgaaaggt
     2161 atccagaata taaattatct tttctttcag aaaacaagat gataaaagtc gagttttctt
     2221 ctcagttttt actgagtaaa gtttattcta tacttttaga gggaacgatt ggttatattt
     2281 tactggattc actttacaaa ggtacttatc aatccttaga aaatcttagt caaaaaatta
     2341 tataagtttg cgtacgattc aaagaaaatt aagagaactt aatactattt tgaaaaacta
     2401 tcatttatct tgcagcttga aaaaaggtaa tccgatagaa ggagaagaat accgtatccg
     2461 atactttttt cacttgatgt attggcagtc attcgacgaa aatcattctg atatattatc
     2521 aagtataaaa aataaacgtc aactattaga aaaatttgcg aaacatgcgc catatacgcg
     2581 tagtatagat agaaagaaat ggatcagcct actaagaatt tcgttgcaac gaatgaaaca
     2641 aggccactcg attacgtatg tcccagaaga gatgaacaaa tttgtgcatc cattgataac
     2701 aaaagaagag tttacgcatt acatactaga gccttttttt gaagcaaact tctggtcgat
     2761 gaatcacatc actccaacag agcaagctta cctttatttt atgtttggtg taatgaacac
     2821 ctatttggaa gaagatttgt tggatactcc agataaagta caagaaagtt ggggctggat
     2881 tgaatcgtct attgtagaaa attttgaaaa aaacttcaag atttgtttta caacttcaga
     2941 aaaaaattat ctatatgtca atttggcaat gatccatttg tattcaaggg tttttgggac
     3001 aaaaaagaag gtggatgctt ttggaaaatc agcagaagac agagattttc agcatatatt
     3061 tccagtgatg tatccaatca tgcaacagtt ttttagtggg gtagctcgca gaattccaga
     3121 gcttaatact tattatgaaa agaacagacg gttgattttt caatactgta tgttagtcag
     3181 ggatattttt ataaaacacg aacaacctgt catattctat attcaatcaa agtatggtaa
     3241 aacacaggaa ttatgggcaa agaaaaggat attatctatg actgaacgcc caataaaata
     3301 tagtgcatcc gctgatcagt tacctgatat tgtcattagc gattatccta tcgacaaaaa
     3361 ggcatatgat caaacgaata cacagatatt ttactggaat ggacaaccta caaaaaatga
     3421 ttgggcgcgt ctgaaagctg aaatcataaa aatcaggaat gagaaaaata gatccatatt
     3481 ggaaaaattg gatttcgtta attaagaaaa gcagattgtt tatgccaaaa ttaagacatt
     3541 gcaaaaagat tattttcttt aaaatcgaca agatgaccag aaagcaagag ttcgttgagc
     3601 tcttgctttc ttttagtttt agaaaggaac atcctgattt tatcttcgct aaatccagtc
     3661 aaaagttttg cagattggtc gtagataatc ggttttttca ataaagaagg ggatgtacga
     3721 atctcaatat atagttcact caaactgagt tcatctattc tgtgaacaag atttttataa
     3781 ctaatagaat gagtagagag catttcggtc acgccgttgt ttgtcaattt tagtaagtgg
     3841 ataaattcat ttttggttag tggttgacga attaagttct gttcgataaa cggtatatca
     3901 tggacgataa gccaacgttt tgcgcgtctt gaagacgagc agccagaacc tgtatacaat
     3961 ttaatcatca tacattcctc caatagctaa gtatttatga aataataaca tgaagaaata
     4021 gtcgaaaagt gccatttaga ggtgtaaaat aagaaaatgt tgtcacgtat aatggaacta
     4081 gctagataag aaatactatt aaatcaagaa aaaagaaaga aggcacaagt atgtcaaaca
     4141 cagccgacgc aatagtagtg atcgatttac aaaaaggggt atgttttgat tcttcgccga
     4201 tacacgaatt agaaacagtg acagcattag tgaataaaag aatcaaaaca tacgaaacga
     4261 ttgggaaacc agttattttc gttcaacacg aagatgctca attagtgcca caaacaaaac
     4321 catgggagct attgtcggaa ttatctattc ctgagcacag ttattttgtg agaaaaaccc
     4381 atgcgaattc attttataag actaatttga gtgttttatt aactgaattg gactgtcatt
     4441 cgatcgaatt atgtggggca caagcagagt attgtataga tacgactgtg aagtttgctc
     4501 atggattagg ataccagctt atgatgtgta gaggagcaac gacaacttat gataatcctt
     4561 ttatgaaagc aaaggaaaca atcgcttttt acgagaaaat ttgggataaa cgatttctga
     4621 catttttgga gcaataagtt gtttcttcgt ttaaaaaaag cagaatggat actggtttat
     4681 ccattctgct ttattacgta aaatgagaaa ataatcctgt tctattttta tgggataaac
     4741 catttatttt ttgtataaaa aatgtttgcg tttacatata attctcctag aaacataaaa
     4801 aggagtgaat agcttgagaa caaaacaagt attaaaggga gcgttggtca tcttatttgc
     4861 tgtaagtaca agcgtcccgc aatggacagt atatggagag agcatttctt ccgaatcgga
     4921 atctcgggag gtagaaacca cagaaactac tgatgctgga aatgaaacga aatcgagtac
     4981 ttcacaaaca gaaacaaata ctgaaacaac ggttgagaca tctgttacta gcacagatca
     5041 agaaacgacc gctaccacag ataaccaaca aacagaagaa gcaacagcta caaaaatgtc
     5101 aaacgacgaa tcctcttctg ctaaagtaag tgacaaagag aatgggacag aaaatgatga
     5161 tatcggtaaa ggtgaaaatt tcaatggtaa agaatatttg aattacttcg gtaaacagat
     5221 gacagaaata cttaatggac caacagaaga catcaagtca aaattaatag agttattagt
     5281 cactgagctt gctgttaatg attatggtgt agataaagag attatcaaac atctgattgc
     5341 tacttatcta ttgtacgata tcgcttttgc aaaaaacagt gtgttgatgt cgatgcttgg
     5401 aggtacagat gatgggacaa cgcctacaga aaacacaggc ttctatccaa atacaaaaca
     5461 acttcatcaa actgtactaa acaaacaaga tggcaaaatg atggatgtat atgcatatta
     5521 tgtcgatcaa ggttcagata aaacagtcat gatccatggc ggatttcgag ggaattggaa
     5581 taacgggatc gttacagaag aatacaatga tttttataag gccgggtata atttattatt
     5641 tgttgattcc agagcgactg gtaatagcgg aggcgattat gtgacctatg gacaatatga
     5701 gtcagatgac gtcctttact ggataaatca agaagttcga gagcggccat ctcaaaagat
     5761 tttactttat ggtggttcga tgggagcagc tacgatgatg tctgtattag cgaaagatat
     5821 tcctgtcaat gtcaaaggaa ttattgaaaa ttgcggattt gcatcaattg atgaacaact
     5881 acgttttacg tacagccaga cagtagcccc cgcactaccg gatgccataa aaaatcagtt
     5941 agacatcatt ggggatcaag agcatgaaga tttatttatg ggccttttga aacaatacta
     6001 ttttgatcag gagatgcatt tggatacaaa agctgctcta cctacgattg gaatgtcaga
     6061 ttcattgcct aaattgatta tccatggaac agcagatgat gtagtacctg tgtctaacgc
     6121 gcaaaaatta tatgagttat caggagggta taaagatctt ttattagtag aaggagcagg
     6181 gcatggaaag gcacaggaag ttgaccatgc tgcctacacg aaacatgtaa cggacttttt
     6241 gaaagtagtg ttccatgatc agataaacgt aaagtatgtg gatgaaaaca ataaaagtct
     6301 actcaaagac caggacgaaa tccagcttta cggagcttat ggagaaaatt atatgacaga
     6361 gcaaaagaca ttcgaaggat acgaattagc aaatgttgaa ggaccgactg aagggatttt
     6421 taatgagacg ataccaacta ttattttcta ttacaaaaaa atcccagtag ctccaccaaa
     6481 aaagcaagat ccgacagaga atgcagacaa taagggaaag aatgaacctc gagaggatca
     6541 atcaagtgat gatacgcaac cgattcagat gacagtgaaa gtacaaaaaa agacagatgc
     6601 aaaaaaagaa tgttgccgaa aactggagaa aagaaacatt cgcttctcat ttattcaggt
     6661 ggatttttat ctgttagcac gattatcaca tggatttggc gaagaaagag agggtaaatg
     6721 aatgaaatgg ttcaagcgaa tagtgattgc agtaccagtt atcatcgtag ttattttagt
     6781 cactgtattt ttgattaatc aaatcacacc aaagccgatt agcttgcttg ttagaaaaca
     6841 atttgacaca agtgaaaaag aacaagtata tgcccgacca gatgatttcc agcaaaaaat
     6901 cgaacaaatc gatataaaaa aagaaatatc ctatccttca aagtacaaaa ataataatat
     6961 ggatatctac acaccgaaag tggaaaagaa aaaattaccg attcttttct ggatgcatgg
     7021 aggcgcatat gtaggagggg ataaaaatga ttgtcgtgat tatttggaat atctatgttc
     7081 agacacgcag caaatcatcg taaatataga ttatgaacga tcaccggaag caaaacatcc
     7141 tacaccagtt atccaattga acgaagccat tcaagcagct aaaaaagaga taaaagatca
     7201 agcagattgg tcgaatattt cgattggtgg aaattcggca ggtgcacaaa ttagtggaga
     7261 atatttgtta gcacttcaaa atgaagaaat taaaaaagct gatcatcttg atccgacttt
     7321 ggagaacaag cagattacga agtttatttc tttgtctggt ctgttggatc cttctgcact
     7381 tactcaagta agtgataaaa tctctagctt tctttatgaa aaatgtggat gggcttactt
     7441 tgataataag gactttgaac aatcagagaa ggtcaaaagt ttagccttgg tcagacatgc
     7501 ggatcgttgg acgcaaaaca cattcttaac ggatggaaat acaaatacgt ttacgaaaca
     7561 aatggaagaa gtaacttctg tatttgagaa agcgggggtg aaagtcacaa aagtagatta
     7621 tgcggagaaa aatgctgttt tgaaccatga atatcagttt gatttcagca ataaacaagc
     7681 gcaagaaaca tatcaaaagt tagtaatgtt tttcaaggag taaagtctta tgatgcaaaa
     7741 gaggttgtaa tctgtcactt agctccaagt cacaaggagc aatttcaata aacaagcttt
     7801 tcatggttta tgaatattgt aacttgtgac tgaggagtta gagatggcaa ggggcacctt
     7861 ttattcgaaa tttgggaata tcacacgatt attgtcacga tctcttttac gtaaatttta
     7921 taagtcagat ttgttgtttt gatcgtttaa aaaaagcaac aaatccgaag cctgcggagg
     7981 caagaaggga caaaaagccg agtggtaagc aaaagaatgc tggttccact aatattccgt
     8041 tgttattgat ataaggctgg gaacaaagat gaatagcaaa taaaatcaat gcactagcga
     8101 aaaaaagcaa agaaatatgg atagctttca taagcagcac ctcctttttt ataattgtat
     8161 gataaataga ttaagaatct ataaatgtga caagtttgtt aggatgtgaa taaaatgatc
     8221 atctagcgaa cttgaaacaa agatggctaa tgataagcaa ttaatgattc agcaatcaga
     8281 taggtacaaa gtttaccatc atacgtctag accaataata gaaaatatgg gaatataata
     8341 gaaaagattt aggatacccc caaaagaatt tccccaaaaa ttcgatgtat cctaaacttt
     8401 tttattttac ataacaatca tatcaactgt gaaataaaat tacaaatgat aacctaaaat
     8461 gaggccaaaa gccagtgtta tcaaggaaat acttaattca ccaaaatttt ttttaatttt
     8521 ttagaaaata agaaacaaaa ataataaaaa aaagaaaaga taaatttttt actactgtat
     8581 actaactttg ttatcgcatc taacaagtaa ctcgaataat accaatacaa ccttctccaa
     8641 agatgcgatg acatacgttc taacccaccg actattgatg ttctgtacca cccttgcaga
     8701 accaaaaaag ccactaccaa atatttggta gtggcttttt ttgttttatg gatttttcgc
     8761 tttttctgtt atggtgctat tctttgattt cagaaatagt atgtcgaact ttagcgatag
     8821 aacgacgtga ttctttttcg ttgtaaaaca tcaaaatcac atatagagag tccaaaatgc
     8881 ttagttgaga aatacgggaa gctaaagctt cagatcggaa ttctgtttct tcagaaatag
     8941 aaataaaaac gacatcgcca agtttagcaa gggaagaatt agcttggcta gtaataacga
     9001 ttgtttttgc tccagttttt cttacggttt cagcaatatg gattgtttct tttgattgcc
     9061 ctgtgtgaga aatcaaaaca gcacagtctt tttccgtcaa taaagaagct tccatcaatt
     9121 gaatatggta atccgtgctg tgagaagttg cgatagggga acgcaaaaat ttatgataag
     9181 cgtcagttgc tacaatctct gaaccgccaa caccgaaaaa atagacacgt ttagattcat
     9241 tgataatagt agccgctttt tttagatcct tttgttgtaa caactttttt gtgtcattta
     9301 aagttgaaat actagactcg aacacctttt gcgccatagt gagctcagta tctgttttct
     9361 gaatattttc gtggatagaa atagcagata gatcattttc ttggatcaac atggccattt
     9421 taaaatcttt aaatccatta taaccaagtt tttttgtaaa ttgaaaaaaa gtagagtcag
     9481 caatgcccag ttcacttgct aaattgctaa taggactatg acttactttg cttggatttt
     9541 ctaaaatata atcggcgata gcctgttctt ttgggctgaa gtcgttgtac attgttttga
     9601 tttgtagtct tatggagacg ctcatgagaa ttcctcattt tcataataag attatcattt
     9661 tttccgttct cattaatgtt aaagatacaa ggagagtagg aaaaaatcaa acaaaaaata
     9721 tttttgtaaa atctatttac aaaaaaattt ttttgtataa tgagggtata agaaatggag
     9781 gcgctatcat ggaaattggt gtaattggtt tgggaaaaat gggattgaat ttagctttaa
     9841 acttaaaaga gcaccatcac aaagtacaag gactcgacat ttcaagtaca gctgttagtc
     9901 aggcaagaga aaatgacatt cctgcttatc aagagatctc tgaatttcta gctacttttg
     9961 agcagaaaaa aatcatctgg ttaatgcttc ctgctggaga gataaccgaa aacatgttaa
    10021 gaaaactttt tccaaaactc ggaccaggag acattattat tgaaggcgga aactcttatt
    10081 ataaagattc aatccgtcga gcagaagagt ttcagaaaaa tgaaatcggt tacttggatt
    10141 gtgggacttc tggaggaatc gaaggagcac gacatcaagc ctgcttaatg attggaggag
    10201 ataaagaaac ttttctatcc gtggaacaat tattcaaaga tgtagcaatt gaaagaggat
    10261 atctgtatgc tggtgcatcc ggaagcggac acttcttgaa aatgatccat aatggaatcg
    10321 aatatggaat gatgcaagca atcggtgaag gattccaact tgtacaaaaa agtgagtatg
    10381 cttttgactt ggaacaagtc gcacgtgttt ggaatcatgg ttcggtgatt cgcggctggc
    10441 tgatggaaat catggaagat cagttaaacg aacatcccaa tttaagtaat tatcggggaa
    10501 tcgtttctgc atcaggtgaa gcaaaatgga cggttgaaac agcgctagaa atggctgttc
    10561 cagttccagt aattgcaact agtttgttca tgaggaatct ttcacaggaa gaagatagtt
    10621 tttcagcaaa agtagtttca gcactaagaa atgggtttgg cggacatgaa atagttgaaa
    10681 agggtgagta aaatgccaga ttatcatata gcaatcgtta attccagcag tttcggaaga
    10741 gtctttccac accacttgga aagattaaag tcagttggag acgttcattt ctttactgtt
    10801 gatcaagaga ttccaggaaa agaactagcg gagttactta aaggattcaa tatcattatt
    10861 gcaagtgtta cgccattttt tcgacgagat ttttttgaaa acaaagatga actgctattg
    10921 atttcccgac atggtattgg ttacaacaat attgatttaa aagcagcaaa agaacaccag
    10981 acaatcgtat ctatcatacc tgctttagtt gaacgaaatg ctgtagcaga aaataatata
    11041 actaatttgt tgtccatgct tcgacgcacg acacaagcac aagcaagagt caaggacgat
    11101 cgatgggaag atcgggcgga gttcgtcggt aggacgctgt ttaacaaaac agttggtgtg
    11161 attggtgcag gaaataccgg aagcggagta atcgaaattt tgcgtaacgg ttttcgctgt
    11221 gatgttctag cttatgatcc ttacaaatca gatttataca tcgaaagctt tggtgcaaaa
    11281 aaagtagaac ttcaagaatt actggaacaa tcagatatca tttgtttatg tgcgaatttg
    11341 acagaagaga actatcacat gatttctgat aaagaagtcg atcagatgaa agaaaatgtt
    11401 tatctatcta atagtgcaag aggcgctcta gttgatgaag aagcgatgat taatggtctg
    11461 aaaagcggaa agatcgcagg atatgccaca gatgttttag aagaagaacc gggacgtcaa
    11521 gatcatccgt atttagcgtt tgaacaagta gtgattacgc cacatatatc ggcctacaca
    11581 agggaatgtt tagaagcaat gggtgaaaaa tgtgtcagag atgtcgaaca gatcagtcag
    11641 caccttcttc ctgaacgttc tgtccagaca aagagcaact atataaaagg atgatgaaga
    11701 aaatggtgaa cggtttaaaa gtgaaaacag gccctcaatt ttatttatat gaagaaggtg
    11761 gtatttcaaa agtttctgat ctcttgaaat catacggcgc gaaaagagta ctggtcaccc
    11821 acggaacggt atcatgggaa aaggcgctac ctaaattagt atttttgaat gacgaaacga
    11881 ttcaattttt ttatcatcgt tattctggag aatgtagtta tgctgaagcc agacgaatag
    11941 ctacaatcat caaaaaaatg aaatcgattt cgtagttggc gttggcggag gaaaactgac
    12001 cgatctagtg ggttatagtg ccaatcttgc aagaatcgat ttcggagtca tccctacttt
    12061 agctagtaat tgtgctccat ggacacctct tgctgtcatg tataaggaga atggacaagc
    12121 agaagggaaa acagaacact tttacagaca agctgctttt tttataacag atccccaatt
    12181 agtgattgat tcccctgttc catatttcat tgccgggtta gcagatacac tagcaaaatg
    12241 gtatgaatct gagctcatct tagaacaaga ttttttgcaa gatgaatcat ttttgaagct
    12301 agcacaagat actgcgaaga tctgcaaaga agagattttg aatcattctg ctaaagcaat
    12361 ccaagatatg gcacaaagaa aactgacatc ggagttcaaa cagctttcag agattatctt
    12421 tgctgttgct ggtttagttg gaggattagg agataaatac gcaagaaatg cggcagcaca
    12481 tgctatgcat gacgctatca gtaaatatat tccggaaagc catcgttttc tacatggaga
    12541 aaaagtagcg tatgggattt tctatcaact ggcactagaa ggtcgctggg aaaaaataga
    12601 cgagttatat cctttgtatc gggaattaga actgccggtt tctctagaac aaatgggatt
    12661 atttccgaaa gataaacaga taatagataa aatggctgaa tttattgatt cgaaagagaa
    12721 agtgcatttg attcctatag aggtaacaca gaaatcactg atacagtcaa tgatggaact
    12781 ggaaggttat atcagaaaga tctgaaggag gaggacacat ggaaacatta agtgtagtac
    12841 aaagtttgtt gattgcttta tgggtagccg cggttatgtc ccgctggtta ggtggaggag
    12901 ccacgttgac cttacgtttt tctcctttga tgactggatt agtcgtcggg ttgatcatgg
    12961 gggaagttgc acaagcgatg attgttacag ccgctttgca aatgatttat atgggggttt
    13021 tttctccagg aggatcaatg ccagctgaac catctattgc agcagctatc gcagtaccag
    13081 tcgcattatt aggggatctg tcgccagaag cagcgattgc cgttgcggtg ccagtcggat
    13141 tgctcggaag ttacttgtat cagtttcgtt ttttcatcaa tactttttta ggtaaatata
    13201 cagacaaagc tgtagaagat ttgaatacaa aagctatggc ccgatcaatt attctttatc
    13261 caacggttgc ctcattttta ttattcgttc cgcttgtttt cattgcttta tatttagggg
    13321 cgccattgat cgctgatgta atcaaagctt tagagggaac agtcgttatc cacattcttg
    13381 aagtagtagg tggagggtta gcggcaatcg gtatcgcgac aactgtatac gttatcggtc
    13441 gaaaagatta tctggtcttt ttcttccttg cttattttat gagtgttgtt ttgaaatctt
    13501 tagatattac gatggttact tatgccattt ttgggattat tatcgctttg atctttgtcc
    13561 agtcgcaaaa agggaaacaa gtacagcctg ccacaagcaa taacaattca gcatcttttg
    13621 atgacgaaga tgactatgat gacggatttt aatttaggag gaatgggaaa tggctgaaat
    13681 aatggataat caaacaggtg ctcctgaaga aatcacgaaa aaagatgtaa caaaagccta
    13741 tctgagatgg cattttgcaa acgaaattcc tcactctttt gaacgttact tggcgccatc
    13801 actactatac ggaatgatgc caatcttgaa aaaactttat aaagatgaag actctttaaa
    13861 agcagcatat aaacggcagt tactgttctt caacactcaa ttaagctggg gtggcggtgt
    13921 gattactggc ctaatgtctt ctatggaaca gcaaagagca gaagaagagt atgaagaaaa
    13981 agagatcttg atgcaggatg acttgatgta taacacgaaa gcgggattga tgggggcact
    14041 agcaggaatc ggagatgcaa tcgattcagg aaccgtccaa tatattttta tcgcaatcgc
    14101 ggtaccttgg gcacaacagg gaagtccttt aggcgcactt tttcctttta tctgctttgg
    14161 gttgtatcag gttttactag gggtattttt cgcaagacaa gctttcacta tgggccgtaa
    14221 tgctacaggc ctaatgcaaa acactggtgt tcagacagcg attgaaatgc tatcagtttt
    14281 agggttgttt atgatggggg tgcttgcggg aaactatgta aaagtagaat cttcattgaa
    14341 atttgcgata tccggacgtg agtttgttat ccaggaaatt cttgatcaaa tcgtgccagg
    14401 gatattacct ttagctgtcg taatgggtgt gtacttgttc tatacgaaaa aaggattgaa
    14461 agtcacacaa gcattattat ggctgactgg aatattaatt gtgctagcag gaatcggtat
    14521 tttataaaaa gaagggaacg tgaataagat gggtgtagtc aatttagcgc gtgtggatga
    14581 gcgtttgatc catgggcaag taatggtaac gttatctcaa aaaagtggag tgaattccat
    14641 ttttgttgta gacgaagttg tagcaaaaga taaatttatg agagatcttt acaaaaatgc
    14701 aggaagccgt acaggacaaa aaacaattgt cattacacca gagaaagcta aattctattg
    14761 ggatgaatat caatttaaag aatacaattg tattttgatc gcaaaaacag tccaaacgat
    14821 ttacgactta gtaaaacacg gaatccccat ggaagaattg aatattggag ggattgctca
    14881 aaaaaatcct gatacagatc ttttagtgac gaaatctgtt tatttaaata aatcagatgc
    14941 tgaaaaattg aaagaaatga atgaacaata tggcgtaaag aatatttatt ttcaagccac
    15001 tccatctgca cctaaaacag gattaaaaga tgtattgaac aagtttgatt tgtcataaac
    15061 aaatgcttag aaaaaagata aagagggcga aaaaatgaag aagaacgagc tgtttcgtga
    15121 ttgggaattt cgttatcgct atatttaccg taagcgccgt acaaaaaaga gtaaacagcg
    15181 ttttctttca gcattagtat cagatattta cagtatgaga acagatgtga cagtcattgc
    15241 ttacgatacg ctagcctacc gatcaaaaaa tatttatgtt ggagatatcg aaaaagcaga
    15301 gaaagttatc tgtacttatt acgatacacc cgttcatgca ttaggaagtt atttcatgtt
    15361 tgattggaaa gaccaaagga aaaaaacaat ctactctata ttattgtcct tcattttgtt
    15421 gttcagttta ggttggtggg gaatgatgat atataacaaa aatcctcatc acgtattcga
    15481 tctgctatcc gtataaacag gtatcactgt actagcattt gggagctact ttttcttatt
    15541 gggaaaagca gctcgcggat ggcctagcag acaaactttt attcgaaata cttcatccat
    15601 attaacgatg ctagaaatga tccgtacgat tgatgatccg agtgtagcgt atgcgtttgt
    15661 tgatgaaggc tgttatggag aaaagggctt agacagcgtg agatcgggta tgaaaaaaga
    15721 ggctattctt ttttatctag actctgttgg ggcagataca ccgctacagt tttcgggaaa
    15781 ttatttttca aataaagaac agtggctgaa acaagttgat aagttgaaag aaaaaaacgt
    15841 caattatatt tttagtgcaa gaaaaaagca agcgcagttc ttctatttga ccaaaacaga
    15901 tttacgaggg aaaacattta attggcaaaa tgccaatcag attattgctc tttttcgata
    15961 agtaaaaagt gcaaaacaag gagtgagaag actaatgttg gaaatcatga tcgttacaca
    16021 tggtaagttg agcgaaggac tgaaagatgc ggcggaagta attgtcggga catcgagcca
    16081 tattgaaaca atgtgcttag agtctgggga ggacatccaa acattcggag agaaggtcaa
    16141 agaaaaatta actatgatca acacagcaga acccatgcta gtatttgttg atctaattag
    16201 cgcaagtccg tacaatcaag cgctcttggc agtcaatgaa ctgactgaag aaaagaaagc
    16261 agccacttat gtcatagggg gcgtgaatct gccaatgttg ttagaagcaa tcaatcatca
    16321 gatgcttcac acaccgatag aagaagcggt ggaacaaatt gtcaaacaag gaaaagataa
    16381 tttaactgtt tggcatgctt cagcggaaga aaaaaaatta gatgaggacg atttttagaa
    16441 ggaaggctga aaagatgaag tggggattta gatggtatgg tgaaaaaggg gattcgatcc
    16501 cattaaaata tgttcgccaa ataccgggaa taaatgggat aattggaact ttgctgacta
    16561 aaatgcctgg agatgtttgg gagatttcag aaattgagga gttgaaagca tctgttgaga
    16621 gccaaggact ggaactgctt ggaattgaaa gtgtagcgat ccacgatgca atcaaggcag
    16681 ggacagatga acgagattac tatattgata actatatacg aacaatcaaa aacttgtctc
    16741 gatgtggggt tcgattgatt tgttatagct tcaaaccaat ttttggatgg gcgaaaactg
    16801 atttgtttaa tgaaaacaac gatggaagct attctctttt atacgatcag aaaatcgtcg
    16861 atcagatgga gccaggagaa atgtatcaat tgatacacag ccagtcaaag ggattcaaat
    16921 tgccgggttg ggaagaagaa cggttgaaaa aattcgctca gctggaaaaa ctgtatgaag
    16981 gaatgaccga agaagaatta tttgaaaatc tgacttattt tttagaaaaa gtgattccag
    17041 tgtgcgaaaa agcagatgta aaaatggcta tccatccaga tgatccgcca tgggaacttt
    17101 ttggttttcc tagagtgacg aaaaatcttg gcgacttgaa gaaaattcta aatgcagtag
    17161 attctccata taatggacta acgctgtgca caggatcttt aggggcaaat ccggaaaacg
    17221 atttagttga aatcatccat gaggtaggtt cgcgcatcca ttttgtccac ttcagaaacg
    17281 tggagttttt aggagaaaga aagttcaaag aaacggcaca tccctctaca gaaggctccc
    17341 ttgatatgta tcatattatg aaagcgttgg tgcaagaagg gttcgacggt cctatacgcc
    17401 ctgatcatgg tagaaccgtg tggggtgaag tggctatgcc tggatatggc ctgtatgatc
    17461 gtgcaatggg actcacttac atgcaaggtc tatatgaggc gataagtaag gaaaaaccac
    17521 tgggagagca ttatgaataa aatcaagctc ttcatctttg atatggatgg ccttttatta
    17581 gaaactggac gtttagctta tcgtgcttat gtaaaatcag cacaaaagta tgattatgaa
    17641 atgcgtaagg aagtctatta tttactaact ggccaaacag aaatggcgat aagagaacaa
    17701 atgggcattt tgtacggaga agatgttcca tatatcaaat ggcgagaagc aatcaatcaa
    17761 tataaagaaa aaatcgtgaa agaagataag agagtatata cgaaaaaagg agcagaagaa
    17821 attttatctt ttgcaaaaga aagaggaatc catacaatcg tggcttcgtc taatacacgg
    17881 gtaaaagtag aaatgtattt aaaaatggaa aatctatatg ccttgtttga tcaaatcatt
    17941 tcaggggatg atgtaaaaaa aggaaagccg gaaccagaga tctttttgaa agcttgttca
    18001 aaaatgaata ttcctccaag tgaagcactt gtatttgaag actcgattgc agggatcgaa
    18061 gcagcaaggc gagcgggcat tttgtctttt ctcatcaaag atcatttgat agggttgccc
    18121 gatcataatg ggaagcataa gttgaaagta gatgtaagtt tatatgaatc caataaagca
    18181 gactatgaat tttatgacct gcatcaagca aaacgatttt tagaaagtaa agggttgtat
    18241 ctctaataaa gaggactatt ttctagtcct ctttatttat tgtcctgcac gactgaggga
    18301 acagatgaaa aagaagtaat tttttatttt gaagtatcta ccttatttgt agtgaacaat
    18361 ttagaacaaa cacaaaacgc atctactgtt atctaccagt agatgcgttt aaagagaaga
    18421 acgagaatcg tccatatgag ggtttccact ccttatgcag atttttttaa ttcttgcact
    18481 gcttgttcga tcgtaacacc tgtcgctaca tgattggtat tttctttgtc aaaaacgaca
    18541 aatagattca aatctgtatc caaggttact cgatattgta tatctttgtt atagaatgtc
    18601 atagccattc tcctttcttt aaatacttgc tgaaaggcaa cgaacgcgtt gataacgaaa
    18661 ttccatttat aagtatacca ccaattgaaa ggttttcata gttaagaaat tttaaagtca
    18721 aaagttgcag ataaaagcaa ataaacaaga aaacaaaaag tgaatcaatc gcggttattc
    18781 aactttttct tcgattcgaa gacaatagaa gacaatgaat tttatatttc attgcattat
    18841 ccttgtgtta ttagaaaaag catgatatat taaatgagta atctattttg aaacgtaatt
    18901 tgagcgatat atctacatag ataaaactcc ttttgcctat caaatattta ttgcaacgaa
    18961 aaaacgtaca ttgatacgta tcaggaggaa tttatttatg aataacggta cagtaaaatg
    19021 gttcaacgca gacaaaggtt ttggctttat cactggtgaa gatggaaatg atgtatttgc
    19081 acatttttca gctatccaag gtgacggctt caaaacttta gaagaaggcc aagcagtgtc
    19141 ttacgatgtt gaagatggtc aacgtggccc tcaagcaaca aacattgtta aagcttaata
    19201 aaaattgact tttaaccgtc ttttcgaaag aaaagacggt ttttttgtac gaaaa
//
LOCUS       DACDYM010000047        19201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDYM010000047 DACDYM010000000
VERSION     DACDYM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.047"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            167..361
                     /locus_tag="IWM59_001732"
     CDS             167..361
                     /locus_tag="IWM59_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979767.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            630..929
                     /locus_tag="IWM59_001733"
     CDS             630..929
                     /locus_tag="IWM59_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979768.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1026..1460)
                     /locus_tag="IWM59_001734"
     CDS             complement(1026..1460)
                     /locus_tag="IWM59_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9979769.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1692..2402)
                     /locus_tag="IWM59_001735"
     CDS             complement(1692..2402)
                     /locus_tag="IWM59_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9979770.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2392..3252)
                     /locus_tag="IWM59_001736"
     CDS             complement(2392..3252)
                     /locus_tag="IWM59_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9979771.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3257..3895)
                     /locus_tag="IWM59_001737"
     CDS             complement(3257..3895)
                     /locus_tag="IWM59_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9979772.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3910..4209)
                     /locus_tag="IWM59_001738"
     CDS             complement(3910..4209)
                     /locus_tag="IWM59_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9979773.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4252..5736)
                     /locus_tag="IWM59_001739"
     CDS             complement(4252..5736)
                     /locus_tag="IWM59_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9979774.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5756..6217)
                     /locus_tag="IWM59_001740"
     CDS             complement(5756..6217)
                     /locus_tag="IWM59_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9979775.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="IWM59_001741"
     CDS             complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="IWM59_001741"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9979776.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7520..8290
                     /locus_tag="IWM59_001742"
     CDS             7520..8290
                     /locus_tag="IWM59_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9979777.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8563..9390)
                     /locus_tag="IWM59_001743"
     CDS             complement(8563..9390)
                     /locus_tag="IWM59_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9979778.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9958..11193
                     /locus_tag="IWM59_001744"
     CDS             9958..11193
                     /locus_tag="IWM59_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9979779.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12192..12452)
                     /locus_tag="IWM59_001745"
     CDS             complement(12192..12452)
                     /locus_tag="IWM59_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9979780.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12479..13780)
                     /locus_tag="IWM59_001746"
     CDS             complement(12479..13780)
                     /locus_tag="IWM59_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9979781.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14072..14530)
                     /locus_tag="IWM59_001747"
     CDS             complement(14072..14530)
                     /locus_tag="IWM59_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9979782.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14543..15868)
                     /locus_tag="IWM59_001748"
     CDS             complement(14543..15868)
                     /locus_tag="IWM59_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9979783.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15885..17297)
                     /locus_tag="IWM59_001749"
     CDS             complement(15885..17297)
                     /locus_tag="IWM59_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9979784.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17314..18078)
                     /locus_tag="IWM59_001750"
     CDS             complement(17314..18078)
                     /locus_tag="IWM59_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9979785.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18182..19057)
                     /locus_tag="IWM59_001751"
     CDS             complement(18182..19057)
                     /locus_tag="IWM59_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9979786.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6110 a         3950 c         2945 g         6196 t
ORIGIN      
        1 aagaacaata gccttttatt atatatatta ttcaaattag ttatatcgag tgaaaaaaac
       61 tttcaaattg attcttttta tttatcataa agatatagat acataatcat tttcacttac
      121 atatatttga atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga
      181 agtaaaaaca aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc
      241 tttacttggt tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa
      301 gaacgagcta gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta
      361 aatccaatgt atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta
      421 tagaattaga agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc
      481 atatgattat ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga
      541 atcaaatata cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat
      601 aatgtatcag tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac
      661 tacttaagca atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag
      721 tgacagagta tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa
      781 aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt
      841 taaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      901 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      961 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
     1021 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1081 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1141 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1201 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1261 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1321 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1381 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1441 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1501 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1561 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1621 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1681 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1741 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1801 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1861 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1921 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1981 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     2041 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2101 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2161 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2221 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2281 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2341 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2401 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2461 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2521 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2581 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2641 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2701 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2761 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2821 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2881 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2941 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     3001 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3061 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3121 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3181 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3241 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3301 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3361 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3421 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3481 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3541 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3601 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3661 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3721 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3781 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3841 atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag
     3901 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3961 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     4021 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4081 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4141 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4201 aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct
     4261 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4321 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4381 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4441 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4501 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4561 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4621 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4681 caagaaccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4741 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4801 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4861 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4921 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4981 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     5041 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5101 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5161 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgcccaat aacaacctaa
     5221 aatcagtccc ataaaaatca atacttgtat cctacctaga tcaggaaagc aaaacagtaa
     5281 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5341 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa
     5401 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5461 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5521 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5581 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5641 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5701 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5761 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5821 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5881 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5941 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     6001 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6061 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6121 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6181 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6241 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6301 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6361 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6421 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6481 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6541 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6601 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6661 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6721 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6781 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6841 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6901 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6961 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     7021 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7081 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7141 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7201 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7261 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7321 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7381 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7441 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7501 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7561 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7621 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7681 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7741 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7801 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7861 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7921 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7981 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     8041 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8101 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8161 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8221 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8281 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8341 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8401 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8461 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8521 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8581 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8641 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8701 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8761 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8821 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8881 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8941 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     9001 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9061 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9121 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9181 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9241 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9301 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9361 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9421 ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata
     9481 aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt
     9541 ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat
     9601 ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg
     9661 acgacagcac acctatcaat taatataatt attttatttt tttatgttct catgtttatt
     9721 tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc aaaaaattgt
     9781 tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat ttagaagttg
     9841 tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg aacgctgtgt
     9901 tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga ggatttcatg
     9961 aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg aatgattgag
    10021 gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc taaaactttt
    10081 gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat ccttacgaaa
    10141 aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa cccttttacc
    10201 aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa gtttgagcct
    10261 cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa taaaaagatt
    10321 cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc agagcatgcg
    10381 ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg gcactggaca
    10441 aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa
    10501 gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct aaatctcatc
    10561 acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg aatcgttggt
    10621 ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt tatacagcga
    10681 aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg caatccgcgt
    10741 atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt tattgcacct
    10801 attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat aacagaatat
    10861 ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg cggggaaggg
    10921 accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat gcaatttgag
    10981 caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca
    11041 gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa gattcgtcag
    11101 caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc aattcaagaa
    11161 atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc ttttttaata
    11221 gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa
    11281 tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc
    11341 ttaaaataaa agatctgacg ctatccaaat gagcataggt agcgtctgcc atttattctt
    11401 agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt caaaagaaag
    11461 accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct agaagcatcg
    11521 ctttgtagag atttgtgccg ctagtcccct caacaattac taaatcaaat gtaggattta
    11581 ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct cgcaatccga
    11641 tgcatactcc tatataaact gccagcaatc cccacatatt gagtagatca tgtcagaatg
    11701 ttctatgtcg tcagcaacga tacactaaaa gtcacgttca ccatgactga ttgctattcc
    11761 cagcacaatt atttctatac acgtttcatg atttgaataa acactcacat catacatatt
    11821 accattcata caccatactg cagcccctac tatatggttg taagctccca aatctatatt
    11881 tgacttgatc actttggtag tagatctact aattctaaat ccattgctga taaaatttcc
    11941 attttttcct ccttctttct gccaaaacag ttaaaacaat aataatcttt atgtaaaaaa
    12001 gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg tcaaaagctt
    12061 gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac aaaaatttaa
    12121 gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg ttacaaccac
    12181 ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat cataaaattg
    12241 atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt catatgcggt
    12301 acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg
    12361 atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt taataaaaag
    12421 ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa aaccaaactt
    12481 agacagtctg gacttcgttt tctccacgac tttgttcatc tgacagcatc tttttatcat
    12541 aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc atgatcaaga
    12601 taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct ggtaatgtcc
    12661 atgttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa taactgataa
    12721 tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc aacatcatag
    12781 gagctccaaa aattatgggc tcattgatat tgaagatacc aggaataata gaaaaacgac
    12841 caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat accacgatcg
    12901 ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga gcgatatagg
    12961 gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc aacatgatca
    13021 gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca aatgaccaca
    13081 acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct aaaatattgg
    13141 ttaaaggctc gaataacgat aataaggcag cgtttacatc aaagcccgcg acgcttcgaa
    13201 tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa
    13261 cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta aagaaacgat
    13321 atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat gctcccaaag
    13381 aactcatagg taagacccag ccaagaggtg cctcttcagg agttaaaacg tttacaggaa
    13441 cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta tctaatccat
    13501 atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc aatcccatcg
    13561 tcatcctaaa gggtaaaaca atcgttgccg aataaggggc aataaattca ttccatccag
    13621 gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat gacaatgttg
    13681 aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt gccattggtg
    13741 ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact ccttttcaat
    13801 aaaatatgaa ccattcattg catgacaacg caaagaatgt tcataactaa ttatataatt
    13861 aagaaatttt atttcaatat attctcttaa ataatcaaat ttatttttaa gaatcctgaa
    13921 aataagtttt ctaaatcata ggagcaacaa aaaaattcga taattaacaa aaatcaaaat
    13981 gattaaatct agtgattgaa acatatcatt tcaaaaaaat tttgtaaatt cacccataaa
    14041 tatcacccta tttaactttt ttcatcgtac actaaaattc aaaacaacta tctttaatct
    14101 ttacttcttg gttttgtttt aataaatttc cttcaaaaat gcctggagaa actacaatga
    14161 tcaccgtatc tttttgtgct tgctccaact tcttcaaatc tataatagct aacagtgtcc
    14221 cttcttttac ctcctggtta ctttcaaccc gtatctgaaa cggacttcct tcgagttcaa
    14281 ctgtatctaa acccatatgg atcagtattt ttctgccaga ttcactttcg atcgttatgg
    14341 catgtcgggt aggaaatata ttatcgatta ttcctttaac tggcgcatgg atatatccat
    14401 catgattcgt tatagcgtat ccaactccta tcatttttga agaaaagacc tcgtctttta
    14461 ctgctgttaa tggaataatt tgaccactag ctacagcttt tagaatgttt cttgttttaa
    14521 agaaatgcac tttttatcac ctctacttta atgctggcca atattttttg tttactttga
    14581 tcatttcatc tagtattttt tttgcgactc tggcatttgg tacggtttga ttcaatgtaa
    14641 atgcttgtaa agctttttga taagaacctt caaagtaagc atctactagt aacttctcta
    14701 ctgcaacttg tgcctccatc aatcctttat gaaaatcact gattggtttt cttagtgtaa
    14761 ttggctccac accagttgca ccaacatatg ccggtatttc aacaactgca tctgaccgta
    14821 gattcggaat cgccccttga ttaggaacga ttagcataaa gcgacgacga tcatcattca
    14881 acaaggaaat agctatatcg acgatatatt gtccatgctc cccaaaatta aagttcaata
    14941 cttcttccac aataccgcgg cgtattttat cagccatttc tttggtattt ttctctcgtc
    15001 catccatgac catattagca cgtgtatact ctttgtcagt atgctcaacg accatatcag
    15061 gatataagta atactctaag taattattcg ggatattact tggaaaattt ttcgtgatga
    15121 ctgacatcat ttgaaaggtt ttttgccaag ttttatctcc tatattaaaa tcggcgactt
    15181 gcatttcttt tgttattagt ttttcaataa tctctggcat aatttctcgt tgcaattctt
    15241 tatcataaat cgatgtatac cagccaaagt ggtttaaacc ataatacgta acgatccaat
    15301 tcttacgatc ataaccgtaa ttcactgcaa tcgtttcttc aattgaaata gtcatatcac
    15361 aggcattaat catttttaca ttcggatatt tacgacgaac agcttcagaa acgatcgact
    15421 ctggatttgt ataattcaaa atccatgctt tgggggcata gtcttgtata tgatcgacaa
    15481 tttccaataa acctttgatt gagcgcatcc cataagcaaa tcctccgagt ccacacgttt
    15541 cttgtcctac taatccatgt ttaagtggaa tcttttcgtc cttttctcgc atctccaatc
    15601 ctccaacacg aatttgtgag aaaacaaaat cgcatcctgt aaaagcaagc tttggatcat
    15661 ccgttgcacg aatgcgaata tttgaaaaac cttttctttt cagcataaat ttaacaatca
    15721 agtacatatc catatttctt tcttcatcaa tatcatataa acgtatttca gataatggca
    15781 accgtttttc attgttcaaa atggcttgaa cgattcctgg tgtatacgta ctcccgcctc
    15841 cagctatagt gataatctgt tttttcatca tcatcttttc ctttctattc ttctaataat
    15901 ttattgaatt ctgccataac ataagtcact tctaagccga tgataatttg ataatttttt
    15961 tcagttggtt taatgatccc gcttatccct aattgcttga ttcgctgttc ctcgaccatg
    16021 gtgggatcca ctacctctac ccgtaggcgt gtattacaac tatagacatc caggatattg
    16081 gcttttcctc caaacgcttg tagcattttt gttgccatat attcataatt tttacttgct
    16141 aaccctaact ctgtttcttc taaggttact tcattgccaa actctgcctt agcttcacgt
    16201 cctaatgtct gaatgtttct cttattgatc aaataataaa aagtaacgta atacaaggca
    16261 aaaaagaaaa ttccgattgg aagaatcaaa atagcattgg tagcgattcg aaaattgatg
    16321 agataatcaa caatacatgc tccgaatgta aaaccaattc gtatattgaa taagtaacag
    16381 acaatccctg ctaaaccggc aaaaagtgca tggatcacgt acaacatagg agaaagaaac
    16441 atgaaactaa attcaacagg ctcagtgata tttgcaataa atgaagttcc tgcaccgctt
    16501 cccatgattc ctttgaccat ctctttattt tctttataag ctgctttata gatagctaga
    16561 catatagctg gtataccaaa catcattact acaaaaaaac ctgataaaaa cagtccagcc
    16621 tgaggatctc cgttgataaa gcgtgtcatc tctcctctga aaacttcctt gctaggagat
    16681 gtatagcttc ctaaatcgta ataaatatac gtattcaaca catggtgcag tccaaagggg
    16741 atcagcaaac gattcaaaaa cataaatatc cccacaccta atgctcccat atcaacgagc
    16801 agttttgcaa aattatcaat cccaatttgg atgagcggcc agataaagcc caaaaaaaca
    16861 gctgcgattg tttgtagaat catcaccata gtcaatggga atttttcgcc cgcaaaataa
    16921 gaaaaaacta ctggtaattt ttgttcttta aagcgattat acgtccatgc cgctactaaa
    16981 ccggaaataa ttccaccaaa aatccccata ttcacttctg ggtccataat ttctaagcct
    17041 tttttcaagg tagcaatcgc taaaaaacca gtaagtgcag gtattccttt gtccttattt
    17101 ttgacaaaac cgattgcaac tcctatagca aataagatat ccaagtttcc aaaaacggca
    17161 tttccagctg caccaattac tgggatgttc agcatatcgt cagctgataa acgcatgatc
    17221 aatcccgcta atggcatcgc tgcaactgga agtagcatcg ctcttccaag cttctgcaca
    17281 tttgatttaa cattcatttt tattcctcct ctattaaata tcctcatatg ttactaactc
    17341 aatcaaagga tcatttttca ctttttgagc aaatgctgaa tgaattaaaa attcgacttg
    17401 gtgggtacga ggatacacaa aacttgaacc atttaacaat tcctgatcca aatacgctgc
    17461 gtgacacatg acttcgatcg ttttatcttc tctcaatatc atgagtaaac ttgccaaata
    17521 ttcttccaca ctcgtattga tttgtcgttt tttttcatca gtttctccta cgacatcgaa
    17581 acaaggctca aaataatcga catgacgaac ttcatctttt cgttcaaagt tgctccgaac
    17641 cggcaaatca tatttttttg ctaatgtaac aactacctcc tgattttcat ccaaagtatg
    17701 agtatgatga tgactgtcta aatgagttgg tatgatcccc gcatgataaa ttttttgaat
    17761 ctgagcattc cattcctgat acagttgatc tttgtcaatg ttaaatggat gctgataata
    17821 agataaggat ctgaactctc cgctttcgat aagagtatcg acagttttga ggagtggttt
    17881 gccacacgtc aatgttaaat ggactcctac acctagccta ggcatttcct ttcttagttt
    17941 cacagcatgc tcgaaacccg gcatatttgc catcaatgta gttgatgtca aaattcctcg
    18001 ctgataagca tccataatcc cataattcac accatggcta tagccaaaat catccgaatt
    18061 aaaaatcact tttcccattt cattctcctt cttttttcta ctaaaatagg cgcaaccaaa
    18121 taatcatagt ccatttacta tgtgtcattt ggttgcgcct atagatagtt acgcccttat
    18181 tttaatagat tcttgctaga tgaattgcca aatagatgat ttcattttct tgaatggcaa
    18241 gttgaaattg ctgttcgatg atatctttaa tctttagtgc tgtacaatac gctttgctat
    18301 gtttaaatgt ttcagtaaaa aaaccaatat tttctactgg cgtttcttcc tgataaatca
    18361 agcgtttaac caggaatttg atatgaacca ttaagcgatg ataagtcagt gattctttat
    18421 cataaacgtt tgaataggat ttttctagat gggacaaaat cgtggtcgtc atttctacta
    18481 tttttaagct ctttaaacta ttaggctctc ctagctcagc attaatgata tgcatagtga
    18541 tgaatcctac ttcatttgac cctaactcgg tttgatatcg atgattgatt cgctcgacca
    18601 cttctactgc tatatcatat tcatttggat aaaggaatct catttctggc aacataattg
    18661 ctgtgacatc tctatcttct ctttttcgtt gaatagcaaa ataaatatga tctgtcaatg
    18721 taatatagat atttgaatct aatggagagg agatcctagc tgttatcagt tcaatcactt
    18781 cttccgcaaa atccataaat tcgattggaa tctgcgagac tatctcactg aatcgtttga
    18841 tcttttcttt agaatctaat acaaataatt tgtcagcatc ctcaacatca aatggcttac
    18901 tcactttagc agaaaagcca atccctttcc ccatcgcaat tttttctatt ccatcgtcat
    18961 cgatcaatac agcattatga ttcagctttt tcatcacata caccgttaat tcacctcctc
    19021 tatatgataa aacaaaacaa cttgcagact ccttcataca tatttttgtc tccaaacaat
    19081 ttttaaagac aaaaaacgac ctaagaaaga accctttctt aggtcgtgcc taattgaata
    19141 gtagcaccct acataattta caaaaatagt ttaacacata ataaaagcgt tatcaatatc
    19201 t
//
LOCUS       DACDYM010000048        18918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDYM010000048 DACDYM010000000
VERSION     DACDYM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.048"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..1364)
                     /locus_tag="IWM59_001752"
     CDS             complement(45..1364)
                     /locus_tag="IWM59_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9979787.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IWM59_001753"
     CDS             complement(1361..2014)
                     /locus_tag="IWM59_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9979788.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="IWM59_001754"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="IWM59_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="IWM59_001755"
     CDS             complement(2724..3440)
                     /locus_tag="IWM59_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP9979789.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="IWM59_001756"
     CDS             complement(3564..3749)
                     /locus_tag="IWM59_001756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979790.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="IWM59_001757"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="IWM59_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="IWM59_001758"
     CDS             complement(5356..6387)
                     /locus_tag="IWM59_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979791.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="IWM59_001759"
     CDS             complement(6394..7224)
                     /locus_tag="IWM59_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979792.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8027)
                     /locus_tag="IWM59_001760"
     CDS             complement(7221..8027)
                     /locus_tag="IWM59_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9979793.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="IWM59_001761"
     CDS             complement(8033..8857)
                     /locus_tag="IWM59_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP9979794.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="IWM59_001762"
     CDS             complement(8847..9947)
                     /locus_tag="IWM59_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9979795.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="IWM59_001763"
     CDS             10125..10910
                     /locus_tag="IWM59_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP9979796.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="IWM59_001764"
     CDS             10943..11326
                     /locus_tag="IWM59_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9979797.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="IWM59_001765"
     CDS             11402..11533
                     /locus_tag="IWM59_001765"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9979798.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="IWM59_001766"
     CDS             11502..11738
                     /locus_tag="IWM59_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9979799.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="IWM59_001767"
     CDS             complement(11816..12787)
                     /locus_tag="IWM59_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9979800.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13439..15061
                     /locus_tag="IWM59_001768"
     CDS             13439..15061
                     /locus_tag="IWM59_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP9979801.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            complement(15347..16723)
                     /locus_tag="IWM59_001769"
     CDS             complement(15347..16723)
                     /locus_tag="IWM59_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979802.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(16723..17379)
                     /locus_tag="IWM59_001770"
     CDS             complement(16723..17379)
                     /locus_tag="IWM59_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9979803.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(17389..18666)
                     /locus_tag="IWM59_001771"
     CDS             complement(17389..18666)
                     /locus_tag="IWM59_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9979804.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(18685..18840)
                     /locus_tag="IWM59_001772"
                     /pseudo
     CDS             complement(18685..18840)
                     /locus_tag="IWM59_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
BASE COUNT         5834 a         3899 c         3060 g         6125 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
    13381 gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag tgatagctat
    13441 gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga ttgatgatct
    13501 agttcctcaa gatcatctcg ttcgaaagat tgatgccgcg cttgattttg aatttattta
    13561 tccaatcgtc gaagccactt actccgattt aggtcgccca agtatcgatc cagtgattct
    13621 gatcaaattg gtgtttattc agtatttatt cggtatccgt tccatgcgac aaaccattaa
    13681 ggaagtcgat accaatgtcg cttaccgctg gtttttaggc tactccttcg aggagaaaat
    13741 cccccctttc tcaacttttg gaaagaatta tgttcgccga tttagagaaa cgacggtgtt
    13801 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
    13861 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
    13921 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
    13981 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
    14041 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
    14101 agaaaaacaa tttgcttatt ctgtccatac ttcatgcgac gacaatggat ttattcttag
    14161 cacaatcatc actcctagaa atattcatga cagtcaagtc gcctttcagc tcgtcaaaca
    14221 atcgaagaga ctttttcctg aaatcaattg tgttgtcgct gatgctgggt ataagacacc
    14281 taagtttgtt cactttttga ctcatttgaa ccttcgacct tgtttgcctt attcaaggcc
    14341 caaagggaaa aaggagctat tatcaaaaaa tgagtttctc tatgacgagt attttgattg
    14401 ttatatttgt ccgcaagatc aaatgctcgc tttttcaacg gttactcgag aagggtaccg
    14461 agaatacaaa tccaatccaa aagaatgtgt gaactgtcct ctgctaaacc aatgtaccat
    14521 aagtaaaaat catcaacgag tcatcacgag acatgtttgg ggagatctga tggatgaagt
    14581 ggaacatctt cgcctaacag atctgaataa atcaatttac aaaaaacgga aacaaacgat
    14641 tgaacgaata tttgcggatg caaaagaaaa gcatgggatg cgttggacca agtatcgagg
    14701 cttggaaaaa gtcgcgacgc atacgatgct agtgtttgct gcaatgaatt taaagaaatt
    14761 agccacctgg ctatggaaag gcaaggagcc tttatttttt tgttcaaaaa ttcgaaacga
    14821 agtagataaa aagctgttcc aagctcgagt tacgagtttg gaacagcttt tgtctacagt
    14881 ctgcgatgtc ggagacatct cgttttctat taggaatcaa ctcaggttca aaagaagcct
    14941 cccggtctct gggaacatca atgtttactt taccaaagct cgttttaagc gtcttattgc
    15001 cataaccgtt tcgtcgattg tcatgtttct ttggctcttt agaatgggcg tcatacccta
    15061 aatggttatt caattctcct tgaagcattt tttcaaataa gggaccaaac acatctttta
    15121 aaacatcttg catatcctct acagattcgg gttgataagt attcaaaatg gattcagcta
    15181 acttctcagc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
    15241 ttttagaaaa ataaaaaccg tgaagcaacc acccgcctag gattgatggt tacttacacg
    15301 gtttatatta cactctcatt tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa
    15361 ttaataattc ttttggattc cttcttaata tattgaatga agaaataatc aatgatacaa
    15421 gaagtactaa actcataaat ataactacat aaatcatgaa tttaggtaat acattgatat
    15481 ctaaacttga taatgtcttg ttaaatcctt cagcttctgc cccaccacct aattggctag
    15541 atgcagattg tttagctatt tgtttagcta tatcaccagt aaccttattc aatatattat
    15601 tcccaagctt gtcagctgta tattgagcta agaaatagga accaacgaat gcggggattg
    15661 atataaatac catctcaatt aggaattgac caaaaatttc taattttgat atacctaatg
    15721 atagtaatac tgctatttct ttctttctag cattcatcca taagaataat aataatgaaa
    15781 ctacaactcc tgcaaatatt aatgagccaa aaaataattt atttgaaatt gagtaaatac
    15841 ctgatataga ttgttgtaat gcagggtaat ttgatgaact tttaatcaaa ttatattctc
    15901 tccaatttat atctaatttc ccaagatctt ttattacact atcaagattc ttatcacctt
    15961 ttacaaagaa tgttgcatct tggtatacag ctgtatcttc ggtattacca taaactttag
    16021 cagctgtatg aatatcagtg attaatgtat tttcgtatag ttcttgtgct gcacttacta
    16081 caccgctatt atgcccatcg aatagacctt taatttctac ttctactgtt tcatctgcac
    16141 ctttctcatt atcagcatca aataaattag attttatttt tatcttatct ccaactttaa
    16201 ggttattttt ttcagctaaa tctttatgca ttaagatttt atttttatct tcattttcta
    16261 aatgctttcc ttctactaat ttatatgctt ctgatacaaa ttttgtttct tttgttgagt
    16321 cgttaacccc agttaacata actgttcttt taaaattctt tgctctttca ggtgattgat
    16381 tcgcaagagt ctcttgagtt tcaataatat cataatcaac taaatctgca acactgttta
    16441 ttctttttac ataggagtct atactatctg tttgagatat ctttttaata tcttcacctt
    16501 ttacatttcc cccacctcta ggtgttcctg gatttacttg tctatttatc tccatagaaa
    16561 aactatttgt tatattagca aatgtttctt ttgaagctct gtccgtagca tctttaatag
    16621 ataaactaat gagacttaaa gttgacatag ataaaataac taatatgata attaatgatt
    16681 ttaaactttt tctagttaca taagcaaatg cattttttat cattattcaa cctccaaatt
    16741 cattttattt acttttttca actttttacc acttagttct aaaatgatat ccgcagaatc
    16801 tgctacttcc ttactatgtg ttacaactat tacacattta tttctatctt tagctaatgt
    16861 ctttaatata ttaattattt ctccagcagt aacactgtct agattaccag taggctcatc
    16921 agctagtatt attggagcat ctgataccaa tgctctagca atagctactc tttgttgctg
    16981 tccaccagat aatttcataa catttctttt tatttgtttt ttatctaaac ctagttcaaa
    17041 caagatactt tcatctgctg atttatttac taatctaata ttttcaattg gtgataaata
    17101 atctattaaa ttatagtttt gaaatactaa agatatatta ttttttctat gattactata
    17161 tcctttattt tgtatatctt cattcttaaa caatattttt cctgtttgag gtttatcaag
    17221 tccagcaagt aaggaaagaa gtgtagattt tcctgttcct gactttccta ctatcgcata
    17281 aaacttccca agttcaaatt tttgatttac tcctgacaaa actttttctt tagaatttgc
    17341 ataactatat gctacattct ttatttctaa tatatccatt attttctcct aacttatttt
    17401 tgataagatt tctttaggct tcttgattaa cattaatgaa gaagcaaata caactgataa
    17461 aacaataata cttattaata ttaaataact ttgtccaagt gttgttatat ttgacataaa
    17521 actactatta tttattaaac ttccaccgga aatcattgaa tcctctgagt taatgaatcc
    17581 atctacaatt acttttagta atacatttcc taaaaataag gaagatatta tacttggtat
    17641 cgatatgaat attaactcga atataaattg cattataatt tgtatcttag atgttccaat
    17701 agataagaat atacctattt cataaattct ttcccttaac catagaatca agattaatga
    17761 aagaacaacc atcccaccta acataataga ataagtcatt acttttatta tatattttat
    17821 tccactcact gactctaaag actcttcaaa cgcattagaa tctttttcaa caaaatactt
    17881 tgactcatca attttaagct ctttcaattt gtttaaggct aagtctgtag attctgcact
    17941 accagaatac attaaaattt tatttgcaat ttcattattt tctgaattat ttaatatctc
    18001 ttggcttgtt gaataatcta caaacaccat attttcacta aaatcagaag ataatcctgt
    18061 atatgtttcc tgttttttac cagaaaagat ccctataatt ttaaatttat gactttttat
    18121 ttttccactt ttttcaatat ctagtaattc aagatcaact tcatcaccta attttagatt
    18181 gttttgttta gcaaattctt catgaacaat aattgaattc ttatcatttt cttctatatt
    18241 tttaccttct ttaattgtaa atactccact actaaataaa atatttcttt tagtattatt
    18301 tgtagcttcg agtgaaacaa cattcttaaa ttcgtcagat aaatcttctc tatttattct
    18361 ttgttctccg ctaactactt tagcatcttt tagttttgct aatccatcat attgaattat
    18421 tttttcttca atttctttta atttttcaat atctttaaat tgattaacat taaaatattt
    18481 accatctttt tttgttattg atattgaaga attagaactt tcatataaag ccttttctat
    18541 ttcattgctt gatttcatta ttgttaaaca agaatacaag caagaaagaa ctatagttaa
    18601 tatgataaaa attattaaag ttctattttt ctttcttgta atatatgcta tagcattttt
    18661 tatcactttc ctaactactc ctctctattt tcttactcca taactaattg catccactgg
    18721 acaagcattt ttacatcgtc cacatctgat acactccaga tgattgcaat tctcaactga
    18781 atctttattc atttggcaaa cttttttaca ttttccacaa ttaatacact tatccttatc
    18841 ctgaatcggc tacactagac tagacagaaa aaataaggtg tgtagaataa taaaaaacac
    18901 actggaggat ttttcatg
//
LOCUS       DACDYM010000049        18616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDYM010000049 DACDYM010000000
VERSION     DACDYM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.049"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            409..1542
                     /locus_tag="IWM59_001773"
     CDS             409..1542
                     /locus_tag="IWM59_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9979805.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1573..1893
                     /locus_tag="IWM59_001774"
     CDS             1573..1893
                     /locus_tag="IWM59_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979806.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(1922..2386)
                     /locus_tag="IWM59_001775"
     CDS             complement(1922..2386)
                     /locus_tag="IWM59_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9979807.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2517..3770)
                     /locus_tag="IWM59_001776"
     CDS             complement(2517..3770)
                     /locus_tag="IWM59_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9979808.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(3799..4116)
                     /locus_tag="IWM59_001777"
     CDS             complement(3799..4116)
                     /locus_tag="IWM59_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9979809.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(4142..4462)
                     /locus_tag="IWM59_001778"
     CDS             complement(4142..4462)
                     /locus_tag="IWM59_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9979810.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(4926..6341)
                     /locus_tag="IWM59_001779"
     CDS             complement(4926..6341)
                     /locus_tag="IWM59_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9979811.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(6358..7674)
                     /locus_tag="IWM59_001780"
     CDS             complement(6358..7674)
                     /locus_tag="IWM59_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9979812.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(7824..8522)
                     /locus_tag="IWM59_001781"
     CDS             complement(7824..8522)
                     /locus_tag="IWM59_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9979813.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(8811..9804)
                     /locus_tag="IWM59_001782"
                     /pseudo
     CDS             complement(8811..9804)
                     /locus_tag="IWM59_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            complement(9885..10718)
                     /gene="msrA"
                     /locus_tag="IWM59_001783"
     CDS             complement(9885..10718)
                     /gene="msrA"
                     /locus_tag="IWM59_001783"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9979814.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(10952..11695)
                     /gene="truA"
                     /locus_tag="IWM59_001784"
     CDS             complement(10952..11695)
                     /gene="truA"
                     /locus_tag="IWM59_001784"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9979815.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(11886..12521)
                     /locus_tag="IWM59_001785"
     CDS             complement(11886..12521)
                     /locus_tag="IWM59_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9979816.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(12668..13909)
                     /gene="sstT"
                     /locus_tag="IWM59_001786"
     CDS             complement(12668..13909)
                     /gene="sstT"
                     /locus_tag="IWM59_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9979817.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            complement(14230..14907)
                     /locus_tag="IWM59_001787"
     CDS             complement(14230..14907)
                     /locus_tag="IWM59_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9979818.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(15158..15700)
                     /locus_tag="IWM59_001788"
     CDS             complement(15158..15700)
                     /locus_tag="IWM59_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9979819.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(15705..15932)
                     /locus_tag="IWM59_001789"
     CDS             complement(15705..15932)
                     /locus_tag="IWM59_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9979820.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(16011..17891)
                     /locus_tag="IWM59_001790"
     CDS             complement(16011..17891)
                     /locus_tag="IWM59_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9979821.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIDYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            18177..18512
                     /locus_tag="IWM59_001791"
     CDS             18177..18512
                     /locus_tag="IWM59_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9979822.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
BASE COUNT         5689 a         3883 c         3096 g         5948 t
ORIGIN      
        1 tttaccaaga ggttatgaaa aaggcgttta gtttcgagta tgaagaaaaa ttttgtgaaa
       61 atggttcctc acgtttttgc ataaatttga cttaataccg agaaactgat cccttctgtc
      121 ataacctctt tttatttatg tctgtttctt tttgaaaaca actatttttt caacgtttac
      181 tctcttgcca atccaacatc atctaacgga tacaactatt tcaatgatta ccattacact
      241 aaaaaatcgg aaacacacct ctccgaacac aaatacataa gactatagaa tgtttcattt
      301 aagaaaaaaa gcgtttcatt atcaaaaagg taagatctcc tttaaaatga aattatctgt
      361 agtactaata atgcagacaa ttttatgaat ctgaaaggag catctccaat gaaagcagtc
      421 acttggcaag gaaaacagaa aatggaaatc agaaacgtag atgatccaaa aatattagaa
      481 ccaacagatg caatcatccg tatcacagca acagccattt gcggctccga tctgcatctt
      541 taccatcatg gtgaagcagt tttggaaaaa gggtatgttg taggacacga accaatggga
      601 atcgttgaag aagtgggatc tcaagttcaa acattgaaaa aagggcaacg tgtggttatc
      661 ccttttaata ttagttgtgg tcattgtcat ttctgcctga atcacatgga aagccaatgt
      721 gatgaatcaa atccagatca gttatatggc gggcttttcg gatttggcaa gttgaacggt
      781 aatcattggg gtggtcaagc ggaatatcta agagttcctt atgcagactc tacttcattt
      841 gttgtaccag ataatgatct tcctgatgaa aaagttttat ttctatcaga tattttgccc
      901 acagcttatt ggagcgttga aaacgcagga gtaaaaaaag gcgatacagt tgtgattcta
      961 ggttctggtc cagtcggtct attcgctcaa aaattcgctg ttatggctgg agcagagcgc
     1021 gtaatcgcag tagatccagt acagcaccgt ctagataaag cggctaatta caatggggtt
     1081 gaaacttatt tcttagaaga cacggcacaa gctggaacag aattgtatga actggctaaa
     1141 ggtggcgcag atgtcattat cgattgtgta ggaatggacg gattagagcc tgtcaaagaa
     1201 aaagcgaaaa atttagtaag cttacaaagc ggcacaatct ctccattaca gatggcatcg
     1261 caagctgtca aaaaatttgg aacagtccaa gtaactggtg tttatatgac tccagcttct
     1321 tcttacccgc tgcaagaatt ttttatgcgg gatatcgctg tcaagcatgg acaggcacca
     1381 attatccatc taatgccaaa aatttacgca atgattgcag aaggactatt cgatcctaca
     1441 caaatcatta ctcattcgct gccactatca gaagcagcac aagcatatga gatctttgac
     1501 aaaaaagaag ataaaaatat aaaagttgtt ttgaaacctt gaattcagta aaaaatacag
     1561 gtgattttct ttatgcccaa aatcaaacga aatacccatg agatcgaaat aggaaaagaa
     1621 gaagatatcg aaatacgagg acgtcgcttc cgcttctatt tccaaaatcg ttacacattg
     1681 atctcactgg ctgtcgattt attaaccggg attttctata tttttggaag ccttgcttcc
     1741 ttaactaaaa tccccgatcg atatggcacc tatttttatt tggctggtgc tattttcttg
     1801 acgattcgtc ctattctcag aatcgtaagg aacattttta tttacgacga aaaaaaagca
     1861 atgaagagaa caagcaaaaa aaaagaggca tgagaatttt tcaaatctca tgcctctttt
     1921 ttcaccctat cgtatcactt tgatacagac gagcggtcgt cccactttct tccttgacca
     1981 ttgtcaaaaa tgtccactcg tatcgttcat aaaacgtggt atgatctgta attaagtaaa
     2041 gtttttcgta cccaagttga tgcatttctt ttctgatttc cgctaatatc cgcttggcta
     2101 tcccctgctt acgcttttcc ggttcaacaa acagggcaca aagatttggt gtcagatcag
     2161 ggcggttatg gaagtcgttt tcaatgacgc ccgctcctcc gacgatggtt tctttttcat
     2221 ctaagaccac ataccattga ggaatacttt ccttattagc gattccagta cgaatacttt
     2281 cctcatacac aacagcagga actccccatt ttgatgaaaa ccaattcgcg ctttgtttta
     2341 ctaattctgg gtgttctctt aatttgatcc agttatatgt aaccaatgtt taaacctccg
     2401 actaaaataa aacgtttaca aaaattatac aagtctttat gaaaattagc aatgacaaca
     2461 atagtgatgt aaaaaaaaaa cggaaaacaa tcacttgttc tccgtttttt gattaatcaa
     2521 gcatctaaag cttcttttct tctttcttgg atcgtagcta ccttgacgaa cggtagatag
     2581 atcaaaatag aaagaaccaa attgacagcg gctagtacac cacctgcaat actttttgtt
     2641 gccaatacac cgccaataat tggtggagtt acccatggag gcataacggt agctgctggc
     2701 actaaacctg tagaagtcgc aaagtaagca acactaacta agaccattgg tgtcaaaata
     2761 aatggaatga acatgatcgg gttcaacacg ataggtaacc caaacatcgt tggttcatta
     2821 atattgaaga cacctggcgc aatcgacaag tttgttacaa ccatatatgg tttgttttta
     2881 cgtcctacta aataaatagc taacagtaaa cctaaagttg cacctgttcc tcctaagttg
     2941 acaaagctgt caaagaaagg tttgttcacg ataaacggtg cgctatgccc agctgcgatt
     3001 gcattaacgt ttgcttcaat ggctggcgca ttgattgttt gcatcaaagg atcgaccata
     3061 ttcgctccgt gtaaaccaaa gaaccataag aatggtgtga taaatgcaag cagtaaggca
     3121 gatggataag agtttgcaag tcccataaat ggttcttgga ccactgtata gaaagaacct
     3181 acgatatcat caacaccgaa tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata
     3241 gtgatcattg ctggtagtaa tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca
     3301 ggcattttga tgatcaattt ttcatttccg cttaaacgta caaagatttc tgttgaaata
     3361 atcccaacaa taatagcaat aaacaaaccg tttgagctca tacctaatgc tccgcctaaa
     3421 gcgaagaaag agcctaaaga aatcacacct gcggaaacac cgtcttttcg gtagccgtta
     3481 gctaaatgat aagcaagcaa gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc
     3541 caagcagcag ccccgaatcc tttccatgat tcaccaccaa aaattgagtt catcaattct
     3601 tggaacccgg gaatcggtaa gttattgatc attactgcta atgctcctaa gatcattaac
     3661 ggcatactca ccataaatga gtcccgaatc gcaactaaat gccgttgtgc accaattttt
     3721 gatgctacag gtataaaatg tttctccata aatgcaacaa atttatccat aaatcttccc
     3781 tcttttcttt actgtagatt acgcgttcaa tcgacgatac agatcaatga tctcttttgc
     3841 taaatctgta aaagctatag atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt
     3901 gacttcaacg tgatttcctt gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc
     3961 ttggactaaa gattcctctg catctttgat tttttcatct gctaaagtga aatcaccttt
     4021 tttcgcagca gcaatcgctt ccattgcatc gctttttgca tttccgccaa acatgatcag
     4081 acccatgatg gcttctagat ttttttgatc ttccatttat tgtatcctcc gataattttt
     4141 attatttgtt tattagtttt tctgcttggt cgagtacttt ttcaccattc atcatgccat
     4201 agtcagccat attgattaca tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt
     4261 gattttgcat aaagcgtact tgcggtccta aaagtaaaac gtctacattt ttagattcta
     4321 gttgattatc cgcatctgaa gcagatacag caaagatgtc tgcttccatt ccttttgctt
     4381 ctgctgcctt ttgcattttt gtgactagga gacttgtact cattccagct gaacatacaa
     4441 gcatgattgt tttcttttcc attttaaaca cgttcctttc tatacttaca cttgtatata
     4501 agcaagtagc gtgccaactt taaaagcgct ttaaaaagtt gatatcatca catttaaggt
     4561 ctataccaag ttgcatacac actaaaaaag aaagcgcaca ctaaacacac aaattaattt
     4621 ttattacctt ttttctttta acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac
     4681 ttttttcaaa taaacggtat tcaaaagctt gcatctgcgg taataagtcc aaccaagtta
     4741 aaaacataag atgttgtttt tactttgcta ttcttattac ttgatgcagg acagcttttt
     4801 cacaacctct caataaacgg tgttcaatca gctgaccctg ggtattagct caaaacgctg
     4861 gattcacaac ctcttttatt tcaaataacc agtaaataca atgaacatct acaaaaaact
     4921 tctatttatt tatagatttc gtcaggcaaa tccaattctc tcttatcaat caaagtctga
     4981 taccacgaag cagatttttt caatgttctg cttcgttcat cgttcaattc gattcgaact
     5041 aatccatatc gattcttaaa tgcattcatt ggtgacacac agtcagtgaa tgcccataac
     5101 atatagcctt cgcaatttgc gccttcttct gttgctttga tcaagtaatg caaatgctgt
     5161 ccgataaatt cgatacggta atcatcttgc acgattcctt tctcatccat atactgttct
     5221 tcatattctc ttcccatacc gttttctgtt atcagccaag ggatattatg ataattttct
     5281 tttagataca tggctatatc atacatgatt ttcggataga tttcccatcc cctggaatta
     5341 ttcattttct tcccaggtaa atcaaagact tcataatatc gttcaggatg gaaaggtgtc
     5401 tcttcattcc attgataacg aggggcgcga acgcgtttag gataatattg attgattcca
     5461 aggaaatcta ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa
     5521 atcgattgtt tttgacacaa ctccaggaac tcttctgtgt agtatccttt taccatcgga
     5581 tcaaaaaata ctcgattata aaacaaatca tacatataag cagctttttg gtcttctgga
     5641 gaattggatc gagcatagac catttcagga tttaaaatac agccaatcct tccaccataa
     5701 tttccgctgc ggtaaatctc tactgcttta gccgttgcta aaactttatg ataattccac
     5761 atcatccact tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc
     5821 gtttggggaa cgattggttc gttgaatgta aaccagtttt ttaccctatc accgtaacga
     5881 tcaaaggcaa tttgagcata cttagcataa aaatcaacta cttttcttga agaccagcca
     5941 tcatattttt ccaaaagaaa agctgggact tcataatgtt ccaaacaaat catcggttct
     6001 acgtctgctt cgatcaaaga gtcgatcatt tcacttatat gctgagcata ttcttcatct
     6061 actatacagt tctcataatc agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt
     6121 gtcagatgga tggatttcat cagatccaca tcctgctggt attgttccat aaaattagtt
     6181 gctacggctg ggccataccc attatgccaa acaaaatgtt catttttata ccagctatct
     6241 ataaacgaat cctgtccttc tttttttcct ttccaacctt ccgtttgcca agcagaagat
     6301 gcagcaccaa gaataaaatt ttttggaata gtaattttca tgatttcctc ctgtttttta
     6361 gatcccatca ttttccacac gatttgctac ttttacaaat ggcagataga tcaaaataga
     6421 aacaataata caaacaagtt gcgtaataac tgcaccgata tttcctgctg ttgccagata
     6481 agcattgacg atcggcggca ttggccaagg gaccattaca actgctttcg ccgcaaatcc
     6541 taaagcggtt gcaaaataac caatcgttcc agtgataaga ggcgttaaga taaacggaat
     6601 agctaaaatt ggattcagca tgatcggcat accaaaaata actggttcat tgatgttgaa
     6661 gatcccagga gcaagggata acttggtgat ttgtttcata tcttcccgtt ttccaactaa
     6721 caatactgca atcagtaaac cgatcgtgac tccagacccg cccatactca tatacatatc
     6781 ccaaaatggc atagtaataa tgttcggtac ttcttttcca gcttcaaaag cttccgtatt
     6841 tacagcaatt gccgctaaaa gcaacggttc ccgtatcggc ttcaccattt gatttccatg
     6901 gataccaatc acccaaaaga cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc
     6961 ttgtacgaca ccttctaatg ggcgttgtac gacattgtaa atgatatcat aaaggtacat
     7021 tcctgtaagt tctttgatga taaatcccgc agccgcgata cccgatacag tcagaatcgt
     7081 cgggaataaa gcagaaaaac tagcagaaac attcgttggc acggtatcag gcattctcag
     7141 ctgtagtttc ttctgtttcc tcaaccagca ataaagttca atagaaagga tggagaccaa
     7201 cattcccaaa aataaccctt tcgtatctgt atattgttta gccaatacgt tttctactat
     7261 aacagtctga ttttcattca ctaattcata aacagttgga ttaacagata aataagacat
     7321 cactgcgatg ataccaggga atacaccatc cactttattc agcttggcta attcgatccc
     7381 tatcagaaaa acagcataga tggtcaagaa acttagtgtg acatagctga tggcagatgc
     7441 gataggcttt aattcttcta aaaaacgcaa gaaacttatc tgtgctaagc cattttccga
     7501 gtcaaagacc attgctgaaa acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa
     7561 tgcagcaaat gcgtttttta tcaccatgat ataccttaaa ttattgattc tcgttgccac
     7621 gttccctaaa acattggcta atttatccat aaaactcatt ccagatcccc tcattcctgc
     7681 ttcttctcct tgacgtcatc tctttttgtc tattagtcag cgcttacatt tcgatgaaca
     7741 gaaactattt actcctcttt tttaccaaat ataaaacaaa aattcatctg atgtatttat
     7801 cataaaatac atcagatgaa ctgtcaatta gtttcttttt ctcctattat tttctgtaat
     7861 tctctccgat ttattgccat atggacagtc attgcatcaa acgcagcaag tgcattccgc
     7921 ctttcgatgg cctgaacgat ctctgtatga atatcgatcg tctcgatttt cagatgctgg
     7981 atatcataat ttttattaaa caaagcaatt gattcattaa tgattgggat aatatgcagc
     8041 aaggctacgt ttccgctagc ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca
     8101 tgttcttgtg tgccttctcg aaaagaagtt tcaatctttt gcgttaattt tttcatttgg
     8161 acgatttgat tcttcgttgc atattccgct gccaaacctg ccatcttagg ctccaataaa
     8221 tatctcagtt caaataagtc tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg
     8281 agtgggtcat tggcaatccc tcgcttatca ctgacaaatg tccccgctcc ttgccgaact
     8341 tcaaggatat tcctggaagc tagagcccgc acagcttctc gcaccgtgct tcgccccacc
     8401 tctaaataat cggacaattc atattcattc ggcagtttat ctcctacttg caactgcatt
     8461 tcctttaaat atctaaggat cttgtcagta gttacttcta ctagggtttt attttcttcc
     8521 aaaccacata cctccttttc atctgatgta ttttttcgta attattataa cacgtttgaa
     8581 tcatctgatg attttttgta tacaaaattg attaatccgt tcatagacat acaaacgaag
     8641 aagatattta ccataaaaaa gactaggata aaattcactc ctagccttcg atcattttta
     8701 ttttgaacga caaaaaatat gatgttcaat aacagacgat cttcattcga ttcttgatgt
     8761 catcttttgt tttttttaat atatggataa aaagtttgat tacatcgttt ctattctaga
     8821 atttggtcga ttctcaattc ttcttctggt gagaaatcta aattatccat cattcgaaca
     8881 ctttcttgta actgacttaa ccgacttgct ccgaccagta cacttgtaac agatttttgt
     8941 cgcaaattcc atgcaagggc catttgcgct aatgattgtc ctcgtgatac ggcgattgtt
     9001 tgcagtgctt tgattttttc cagagttgat ccaacttgtt cttctgaaag gaatggtatt
     9061 tccttacgat gtgctctaga atcttcagga ataccatgta gataacgatt tgtcagcaac
     9121 ccttgagcaa gcggactgaa cgtgattgcc cctagccctt cttcttctaa tactggagat
     9181 agatcgtctt ctagtagtgc acgagaaaac atattgtagc gcatctgatg aataatgaac
     9241 ggtgcttcct ttctttttaa aatttcagtc atttttttgt atcttctcca ttatagttgg
     9301 atataccaat atataatgct tttccttgtc ttacaagttg catcaacgct tctgctgttt
     9361 cttccaaagg tgtatcaggg tctgggcgat gatgatagaa gatgtcaaca taatctagcc
     9421 ccattctttg gaaactttgg tcacaacttg atatgatgga tttacgagag ccccattccc
     9481 catatggccc ttcccacata tgataacctg ctttgctaga aataatcagt tcatcacggt
     9541 aggcttgaaa atcttctcta aaaatacgcc caaagttttt ctctgcagct ccagcagggc
     9601 ctccataatt attcgcaagg tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa
     9661 tctctctttg attatggata ggatcataat caccaaaatt ttgccataat ccgagaggag
     9721 agataaaatc ggtaatttta aacctgaatg accggattta cgatattcca ttttttcata
     9781 acgtgcacta tctgcaatat acaatttttc tcctcctcat caaatctttt taaaacgctt
     9841 tcttgttttc tatttttagt ttactacatt ttaaagaaaa ggagttaatc aaaatgattt
     9901 tttcgtatat ttttttgcca tttgtttttt actcgttgat agatcagaaa ttgattacgt
     9961 gcttgctgga tttttttata gcgtttcggt tgttttttgt aaaattgctg atgatagttt
    10021 tctgctggcc aaaacgtacc ggctggttcg atttttgtga cgattggttg gtggaaagtt
    10081 cctgaactgt ctagtttctg ttttgattgt tccgctaatt ctttttgtcg ttcgtcttga
    10141 taaaaaatca ctggacgata ttgcttccct ctatcttgaa attgtccaaa tgtatctgtg
    10201 ggatcagtaa tttgccaata gattgtcagc aactcgctat aacttatttc tcttgtgtca
    10261 taaatgatct ccacagcttc cacatgacct gtgtaaccac cacttacttg atcataattc
    10321 ggtttttcaa catgtccgcc cgtgtatcct gacaaaacag ataggatccc ttttttcgat
    10381 tcaaacggct cgaccataca ccagaaacat ccccctgcaa atactgcacg ttcttgatag
    10441 tcagcatctt gcatggcatg cttggaaaag tcatattttt gctccttatc aaatccttcc
    10501 gtaatcaata aataaaagtc cattacatca ggcgtcagat tcattcggat agctaatgga
    10561 cgtagtgtta cttctagttt tgataactgt tctttaacgg atatattttc tcttgtgtca
    10621 ttctttgtct gaatcaatac ttttcgttcc caatctcgtg tattcggatt caaaatcaga
    10681 ttataaagtg tttgaatgaa tatttgttcc tcatccatag attctcctcc tttccgaaga
    10741 agacaagcta gtgactttag ctgtaattta tcataaagtg aggaaataat cgattgaatc
    10801 gtatttattc aaagaagata agcagcttca gatgatataa gatgatcttt atcttaaaaa
    10861 gcatactcta tggtttggtt taactaaaaa acgacctctg aaaaaaggga tgttctccaa
    10921 ttctcagaag tcgagtaaag atgattcttt tttatgagcg ataataaact tcatccaaaa
    10981 acagaccttg tgcagggacg gtttctcctg catgttgccg cactttgctt tcaaatattt
    11041 catcgattgt ttctatcggc atggcacctg tgccgatttc tagtaatgtc cccatgataa
    11101 tccgcaccat tttatataag aatccgtctc cgacaaaagt aaaatgaagc atttctccct
    11161 ttcgttcgat agataattca tcaatcgtac gcactgtgga tttttttgat tttttcaatg
    11221 cagaaaaacc aatgaaatca tgtgtaccca ctaatttttc gcaagctgca ttcagcttgt
    11281 ccatatccaa ctcttgaggg aaataaaagc tgtggtttcg ctcaaatgct gaaggaatca
    11341 cgttgttcca aacataatag ctgtattttt tgcccaccgc attatatctt gaatggaagc
    11401 gctcaggcat ttcttcgatt cttttgatga caatatcccg cggaaggtaa cgattcataa
    11461 agtcgatcat ttctgccagt ccaatttctg tgtttgtttt aaaatttgct acttgtcctc
    11521 gcgcatgggt gccagcatct gttcttcctg atccaataat ttctatctct tcatttgtca
    11581 tctgatgcaa tactttttcg atttttcctt gaatggtttt atcgtcatct ccaagtcttt
    11641 gccatcctga atatcttttt ccgtcatatt cgattgtcaa cttgatgttt ctcatatttt
    11701 agtggctcct ctgtcttcca tttggtcttc tctctacctt tttaagattg tagcacaaaa
    11761 tatagctcct tgcttctcta agacagaagc tatacaagaa aactgtggaa atatatattt
    11821 cagaaaaaca aaaaacagcc accggatgaa gtatccgctg actgttcaat ttgacggctc
    11881 aagttttact gtttgagatg caagatactc aaccaggcaa tgggcacata attaaagaaa
    11941 aaactgattc tttggcaaaa acctatttta ttcagtaatg gccaagaatt tatagaagga
    12001 attcttgcca gtccataaat cgccgcaaaa agcaaactga tcacagacag tccaaggtat
    12061 aagtggctta cgtccatttg cccatttttt cgatagatca agaaaagaag aactggaaac
    12121 aataaaaaac cactgtaacc taaacctgat ccgatattat ggacccatgt cgagacattc
    12181 cacgaagatt catgggtatt gatactgaac aaacctgtaa agatacaatc acctatacca
    12241 tagaacgcga tggctgctgt aactagggta gctaacacac gagatgtact tacaaatgca
    12301 gcatacaatg caggcaatga caggatgaaa aagatccctg aaaccacaga ccaaataaga
    12361 aacgcttttc ttacaggact ggaaacctct ccaaatacac tgatcacgct cttcatttga
    12421 tctaattcag gataatacaa tcctaatacg tacggagtca aaaaatcact cagcacgcct
    12481 aataataaaa agaaaaaacc aaatttacgt aaaaaatcca tgatacgcct ccaataacaa
    12541 ttacctatgc ttttaattgt tattataaac gactttatct tgatttaaca gaaacctcta
    12601 tttacacgaa catctatata actcattaaa aactcgaatc attttaaaaa tactaaatat
    12661 ctaacgttca acgttcactt aatccatcta aacaatcttt caatttgatt ttttccccac
    12721 ttcttttacg cgcgcctagc tctcctattg cagtaaacaa cagatcactt gacgaattta
    12781 aggcagtttc cattgaatcc tggataacac cgataataaa tccaatacca acgatttgca
    12841 tcgcgacatc attagagata ccaaacaaac tacatgctaa tggaatcaat aacagtgaac
    12901 cgcccgcaac acctgaagct ccacaagcag ctaaagcaga caaaatacat aaaaggactt
    12961 tcaaaacaaa tggtgcttca atccctaaag aatgtacagc agtcaacgtc atcaaggaaa
    13021 tggtaatcgc ggcaccgccc atattgatcg tagcacctaa cgggatcgat atggaataag
    13081 actcttttgt caatttcaat ctttcagcca acatcatgtt tatcgggata tttgcggcag
    13141 aacttcgggt aaagaaagcg ggaagtgcgc tttctttcaa acaaaagaaa atcaatggat
    13201 agggattttc tttaataaac aaaaacgtga ggaatgggta cacaactaat gcgacaaaca
    13261 gcatcgtacc gatcaaaaca agtaaaagct gcagatactc tgccaatcca gcgattccag
    13321 tcgtagcaat tgattgataa accaatccta aaatccctat cggagcaaat gcaataatca
    13381 cttgtacgac ttgtgacaac gctatagata tctgatccac caactctttt gtcggttctg
    13441 aacgtgctcg aaaagctagt cccaacaata cagcccagaa taaaacgcca aggtaattcg
    13501 catcaataag agattgaaca ggattttgtg tgacattcgt caacatagta gataaaacac
    13561 tttccaaact ttgcggtgct ttttcagata ctgtttcttg taaagtaata ggaacagtaa
    13621 aaattgaact tacaatgact gctacaagtg cagaaagcaa tgttcccact aaatataaga
    13681 tcaaaatagg cttcacgaat gtcttattcc caattttatg cttggcaatt gacgccatcg
    13741 tcaagaaaaa aaccagaata ggtgcgattg ccttcaagca actgacaaac aacgtcccca
    13801 atattgaaat aaatgtccac gtggggaaaa agataccaaa caatattccg actaaaatac
    13861 caacaacaat acgttgaact aacgacatat ccaaaaactt ctctaacaaa tgaccaccct
    13921 ttcacaattt taacaatcat tgtatcattt tatctaaaga aactaaaggt gcaattttct
    13981 aaaaattcta ataatacaat tggcaattca tgctcttgca acattcaatt ttagtattgg
    14041 agggtttatt caatctctaa agaagtcgag agaaaactat ttatcgttat tattatagtt
    14101 atccaagtaa aacgtaacta agatgaagga cgaaactaga aatttcatcc ttcatcttaa
    14161 ttcttagaca tagcaatcaa ttaatatttt ccactgagtc tcttctttct ctcactctca
    14221 ctggtttact tacccataaa cgatttgatt aaagccattt ttcaattcat tattcgctgt
    14281 ctcgttaaag taaagataat tatcgggatt cccctgattt tgttgtgtca ccaatacttt
    14341 tggctgattt tgctgaaacc cgaatagata gacgtgcttt ttcagatatg gttctgtctc
    14401 tgcatcggaa tagacggctg tgatttgata gtctgcttga cctgtcccac tttctgacca
    14461 ttctgcagaa acaggcactc caccaatagc catcttccac tccccgttca aaataacttg
    14521 agggacttta agaccatata agtccacact catttgattc gtatagcttt tgtattgttg
    14581 tcccattgtt tggccccatg tagccataaa tgcctctaat tgtgcggatt tgtctgcatt
    14641 ccacagtgtt tgttcgactt gttcgctgct ttctgaagat gaagcctcta cagaagaact
    14701 tgaagtagat gtttgagttg tttcagtcgt tttggaagac tctgacgaag tagaggtatc
    14761 tgctgtttcc gtacttgctg cagttggctt gctctctgtg ctagttgttg tagtagttgc
    14821 aattgttgct ttcgctgttt cttttgttga ttcttttttc gattcattcc cgttggtaca
    14881 accagctaac aaaaaagcta gaccaattgt tactaaaatc ctttttttaa attaaaaaac
    14941 tcctttcgat tactcccact tctttattct atcaaataag caatgtgatt ttattgattt
    15001 tttgtatttt ctcatccatc ttttaaacct tttaaaaact aagaagcacc cgctgatgtt
    15061 gtctgtaata catcagcggg tgcttctttt attttaatct tcgcgatttt ccaatttacc
    15121 tcacgcttta tatttgtatt tgaagaaaca tagactttta gtcatcatcg tcttcctcat
    15181 ccaaatcttg tcggacagtt tttccttgga gtctttgtac ttggtagatg atttctttat
    15241 tgaaacttaa aagttcaaca agcaattgtt tcaagccata tttttcttct ttttcagcta
    15301 atttgattgc ccgtgtgata aagacatttt ggaaatcaaa atcttttgca aagttttcca
    15361 acattgttga ggctggtaaa tatttgtttt gaccatgttc gtaaattttt ccataacgca
    15421 tgacttcttc taaagtagac ggaacgattt cattttcaat cactaactca tgagagattt
    15481 tattgaacca tctcgcttct tgatcagcat attctgaaaa aagggtactt aattcttgcg
    15541 aagatgtacc caaaacatac aattgtgctt ggatcaattt catttcttct aatttaaaat
    15601 ttgccaatac atgccccacc atggcgccag cagttggttt atgatgatcg atatctgctt
    15661 gttttgtttc ttgagcaaat ttttcttcca ctatattcac tgatttacgc ctcttttact
    15721 tttgttgatt ggacttcata acctaatttt tccacaactg cagctaattg ttcagggtgg
    15781 acttcatctg gatcaaagtt tgctttgatt ttgctcgcat taaacaaaac tttcgtattg
    15841 cttaccccag gctgttgaga aaccgcatgt tctatttttt gcatacagct tggacaagct
    15901 agtgtatcta attgcataat catttttttc atcgtagttc ttccttcctt tttcttgata
    15961 gtcttattct agattacagc ttccataaaa aaattgattc ttatcaagtt ttacgatttt
    16021 tcttctttgc tttcgtgtcc aaaatacaat aaacgcatcg cgttaaagat aacgatcaag
    16081 atactagctt cgtgaacgaa catcccacta gccatgtaaa taaacccggc aaataagccg
    16141 atgagtaaaa ataagacgac accaatggca atcactacat tttcacgggt attcaaaaca
    16201 gttttcttag ccaaacggta agcatgaacc aacgatccaa aactagattg catcaagaca
    16261 acgtcactcg tttcgatagc gacatccgtt cctgatccca tagcaatccc gatatcagcc
    16321 gcagccaaag agggagaatc attgattcca tctccaatga aagcaacgtg caatccttgt
    16381 tcttggtatt ttttcacaaa ttgaaccttt tcatctggca ataattcagc atggacttca
    16441 tcaatgccta acatgtcagc tacataatca gcagtcatct gattatcacc tgtcagcatc
    16501 actaaatgtt ttgctcctgc ttgtttcaac atagctaatt gttcagcgac ttctggtcga
    16561 atcacatctg acacaccaat gatttgagtg atttttttgt cgattgctac aataatgact
    16621 gagctgcctc tttgttgtaa ttgatgtaaa tcttgtactt gctttggtgt gagagaaagt
    16681 ttgtgagcgg caatcaattt ggtgttgccc gcacaaactg tatgaccatc aatctcagct
    16741 gtgattcctt gtcccttgat cgtctgatta tctttgactg ctaattgttg aaaatcaatg
    16801 tctttctcct gagcataatc gatcacggcg cgccccagag gatgatcgga taacgtttct
    16861 aatcgagcca ctaaagctaa gctgtcccct tcatcctctg tatagttctt gatagtggat
    16921 acagcagtag ttcctttggt caatgtaccg gttttatcaa agacaaaagt atcgattttg
    16981 gaaaaagtat ccattgcttc accgcctttg actaagatgc cccgtttcgc tccattaccg
    17041 attcctgcta cattcgaaac aggcgcacca atgactaaag cgccaggaca accaagaacc
    17101 aaaacagtga tggctaaacg aaaatcacga ctgatcagac caacaagcaa cgcaataagt
    17161 aaaacggttg gagtataata ctgagaaaat tgatcaatga atttttccgc atgtgatttg
    17221 gtatcttgtg cttcttcgac taattctata attttggcaa aggtcgtatc atctccgacc
    17281 ttagttgctt cgatcgtcac gtagccatct gctaaaatcg ttccagaaaa tacgttatct
    17341 ccactgattt tgacgacttg tcgtgattca cccgtcactg cggcttcatc cgcataccct
    17401 ttcccgtcaa tgactcgtcc gtccacagga atagctccac cagtttttac taagacgata
    17461 tcgccttctt ccacgtcatc gatttccact tcttcggtcg ttccatcttc ttggacaacg
    17521 ctggcagtcg atggggccat ctcggtcaaa gccttgattg attcccgcgt tttggtcaat
    17581 gttttttgtt ccagataact accaaacaaa aacaagaatg taacgatagc tgactccttg
    17641 tattctccga tcataaaggc accaagcaca gcaatagtaa cgagtaaatc aattgagata
    17701 actttgactt ttactgcctg atacgcatgg attataatag gtatgaccgc aataatagaa
    17761 gctatggcta gagcaatgtt atagaacaag acaaatccag taaatttccc gataaaacca
    17821 aaaataatca acacagctga taaggcggtg atatgatttc gatgctcttg gatccacttt
    17881 tgtaatttca tctttctccc tcttttcctt ttcattctta cgagactaag tatagatact
    17941 tatccatcat aaaaaattga ccactatcaa gtttattcaa aaaggcggtt ttcttttggt
    18001 atactgaatt cctacagtca ttaaacgatc atatagaaaa aggaagcgcc gaaacgcttc
    18061 cttaggtagt tctatttttt tattcaaaga taagatgctt tgtcatctta cgactgaaga
    18121 acaacaatca aaaaagggca gcagccactc attcacaaga aatatcgttt tttgatatgg
    18181 gagttgaagc agtggacaaa tatctctact tattagctgg gaataagatc caaaaaagct
    18241 taatggattt tatccaagaa ttggaatgta ctttccacaa aaaattcact cattccattc
    18301 ttttaaaact tttgattcat acagcgtgtc taattgagcg tacattgata aacggtcatg
    18361 aacttaaaat tatatcagaa gatgacacaa gaccttctca tgaaacaatt tttcatgtaa
    18421 aaaaggcctt caaaaatatt gaaacagaat ttgggatcac tgtttcttat gatgaatgtt
    18481 tctttatcta tgatattatc gcatctaaat aatctttttt tatgtaaacg agtattttgg
    18541 gagcgtcaat aattttgtgt aaataaattg tcctcctgca aaataattag ttactcagta
    18601 aacattgaaa ctaatg
//
LOCUS       DACDYM010000050        18437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDYM010000050 DACDYM010000000
VERSION     DACDYM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.050"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(315..398)
                     /locus_tag="IWM59_001792"
     tRNA            complement(315..398)
                     /locus_tag="IWM59_001792"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="IWM59_001793"
     CDS             926..1426
                     /locus_tag="IWM59_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9979823.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="IWM59_001794"
     CDS             complement(1616..1960)
                     /locus_tag="IWM59_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9979824.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2114..3034)
                     /locus_tag="IWM59_001795"
     CDS             complement(2114..3034)
                     /locus_tag="IWM59_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9979825.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3221..3712
                     /locus_tag="IWM59_001796"
     CDS             3221..3712
                     /locus_tag="IWM59_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9979826.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3736..6111)
                     /locus_tag="IWM59_001797"
     CDS             complement(3736..6111)
                     /locus_tag="IWM59_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9979827.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6437..7063
                     /locus_tag="IWM59_001798"
     CDS             6437..7063
                     /locus_tag="IWM59_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9979828.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7086..7790
                     /locus_tag="IWM59_001799"
     CDS             7086..7790
                     /locus_tag="IWM59_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9979829.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7982..8815
                     /locus_tag="IWM59_001800"
     CDS             7982..8815
                     /locus_tag="IWM59_001800"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9979830.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9012..10940
                     /locus_tag="IWM59_001801"
     CDS             9012..10940
                     /locus_tag="IWM59_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9979831.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11078..11533
                     /locus_tag="IWM59_001802"
     CDS             11078..11533
                     /locus_tag="IWM59_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9979832.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12003..12272
                     /locus_tag="IWM59_001803"
     CDS             12003..12272
                     /locus_tag="IWM59_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979833.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            12672..12926
                     /locus_tag="IWM59_001804"
     CDS             12672..12926
                     /locus_tag="IWM59_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9979834.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13015..14061)
                     /gene="holA"
                     /locus_tag="IWM59_001805"
     CDS             complement(13015..14061)
                     /gene="holA"
                     /locus_tag="IWM59_001805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9979835.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14099..16372)
                     /locus_tag="IWM59_001806"
     CDS             complement(14099..16372)
                     /locus_tag="IWM59_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9979836.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16365..16904)
                     /locus_tag="IWM59_001807"
     CDS             complement(16365..16904)
                     /locus_tag="IWM59_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9979837.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17040..17258)
                     /locus_tag="IWM59_001808"
     CDS             complement(17040..17258)
                     /locus_tag="IWM59_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9979838.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17255..17500)
                     /locus_tag="IWM59_001809"
     CDS             complement(17255..17500)
                     /locus_tag="IWM59_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9979839.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(17616..18344)
                     /locus_tag="IWM59_001810"
     CDS             complement(17616..18344)
                     /locus_tag="IWM59_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9979840.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5648 a         3545 c         3225 g         6019 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat
     2041 tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa
     2101 taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg
     2161 ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta
     2221 aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt
     2281 cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag
     2341 acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg
     2401 gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta
     2461 cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga
     2521 agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc
     2581 cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt
     2641 ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa
     2701 tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat
     2761 tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag
     2821 ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata
     2881 aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa
     2941 tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt
     3001 tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc
     3061 tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac
     3121 tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag
     3181 atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt
     3241 cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa
     3301 agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag
     3361 caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt
     3421 tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca
     3481 cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa
     3541 cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca
     3601 agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt
     3661 attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa
     3721 cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa
     3781 acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg
     3841 ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat
     3901 tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc
     3961 aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat
     4021 cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa
     4081 tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat
     4141 caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt
     4201 catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac
     4261 cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc
     4321 agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt
     4381 cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag
     4441 tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa
     4501 actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct
     4561 tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc
     4621 gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg
     4681 aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc
     4741 gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc
     4801 ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa
     4861 acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc
     4921 ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc
     4981 aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt
     5041 tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa
     5101 tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt
     5161 gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc
     5221 attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc
     5281 tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc
     5341 atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc
     5401 ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt
     5461 aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag
     5521 ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt
     5581 gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt
     5641 tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc
     5701 ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact
     5761 atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa
     5821 atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt
     5881 gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt
     5941 ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat
     6001 tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg
     6061 caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac
     6121 ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa
     6181 acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg
     6241 atgaatctaa tgtaacaagg acatattaaa atgctatgaa aacttacttt ccttctcttt
     6301 agaatatcca aatacttttc atatagtctg ataattcaga taagtaaaga gaaataagta
     6361 cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa gttttggcta
     6421 tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg aagaaaactg
     6481 ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag atgcggaacg
     6541 aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc ttacccatac
     6601 tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa tcccagttta
     6661 tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt ctggactcgg
     6721 tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa
     6781 aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc attctatagg
     6841 aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt ttagaggaag
     6901 tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata gtattcggac
     6961 ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg atgcaacgac
     7021 gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact taggaggaga
     7081 ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaacgatg ttcaatacaa
     7141 ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc
     7201 attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct agtgacagtg
     7261 gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga aaagaaattc
     7321 cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa ggtggatatg
     7381 acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat gatgaaatgt
     7441 ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta gataccgctg
     7501 ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt gaagatatcg
     7561 cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat ggtttgacga
     7621 tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg cttttaaatg
     7681 gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc atcaatgata
     7741 cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa tggggatatt
     7801 ttaattttta ccagagaatc ataaaaggca aagtctgaac gagggctttg tcttcttttt
     7861 tctcttcgtg tacttatctg ctttatttct caagtttttc ttaatattaa aaaacagatt
     7921 gggtttctca aaaaataacg gtatactata agatgtatct taattgggaa ggaaggaatt
     7981 tatgtttttt gcaaatatta tcaaagcagt gattctcggt attattgaag gaataacgga
     8041 atggctgccc atcagtagta ctggtcattt gatcctagca gatgaattca tcaaactgga
     8101 tgccagttca caatttatgt cgatgttcaa tgtcgttatc caattagggg cgatcttggc
     8161 tgtcgtcgtt ctttattttc acaagttaaa cccattttct ccaagtaaat caacggtaga
     8221 aaaaaaagat acgtggacac tttggtttaa agtgatcgtt gcttgcatac cagccatcat
     8281 cattggtttg cctttggatg attggttaga agcaaatttc cacaagttct taccagttgc
     8341 cattgttttg atcgtttacg gtattgcctt tattattgtc gaaaaacgca acaaaaacaa
     8401 aacacctcgt tggacttctc tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt
     8461 attccaagtc ttggcacttg ttcccggaac atcccgttct ggcgcaacga tcctaggatc
     8521 tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt tcatttttct tagggattcc
     8581 tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag ggaattcatt
     8641 caaaatggaa gaaactgttg ttcttcttgt aggaacattc gtatcttttg tggtttccgt
     8701 cttagctatc aaattcttac taagctattt gaagaaaaac gactttaccg cttttggctg
     8761 gtatcgtatt atccttggta tcattttgat tggttattgg tttattgcaa tgtagaaaaa
     8821 accagaacac ttctcttatt tgagaaatgt tctggttttt tattttcttt aataataatc
     8881 attataaata ataaaaacaa catcaagctt aatcaccaaa tgataaccat tatcgttctc
     8941 atttagctgt tattattatc aattacattt ttattatgct atccttgttt tgacaaggag
     9001 gtctaataac gatgactaat cagaaaaaag cttttgcagc aacaatcggc tgccttttat
     9061 ttatgatctt cggttttatc ttcgaccgat tctctcttac catcacccca cttttttata
     9121 tcagtgccat tatcatcggt ggatataaac aaacatcgga aggcttgaaa gaattgtgga
     9181 atgatcggac actgaatgtg gatttattga tggctttagc tgctattggt gcctgtttga
     9241 tcggtaatta ctttgaagga gcaatgttga cattcatctt ttgtttaagc ggtgcattag
     9301 aggaatacac aactaataaa agtcaaaaag aaattactgc tttgatgaat cttcaaccac
     9361 aaaaagccca attattaaaa gaaaatggac aaatgcaaga agtagatgtt tctcaactaa
     9421 aggtggataa cttggtattt gtcgctaaag gagcagctgt tccgatagat ggctttttag
     9481 aaagcacaca ggcaacgatc gatgagtctg cattaagcgg tgaatctgtg cctgtagaaa
     9541 aatatttagg cgatgagatg tatgcaggaa cgctgaatca aggaaatccc atcacgattc
     9601 gagtaacaaa aacgagttcc gaaactgtct ttgctaagat tatccaatta gtagaagaag
     9661 cgcagaatac gccaactcaa acagcttcat ttatcgaacg aatcgaaaat aattatgtaa
     9721 aattgatttt gcttgctgtt cctctaatga tccttctccc ccactttttc ctaggttgga
     9781 gttgggatga aagcttttat cgaggaatgg tattattagt tgtggcttct ccttgtgcgc
     9841 ttgtcgcatc tgctacacca gcttctctag ctgctctttc aaattctgca aaaaatggga
     9901 ttttaatcaa aggcggtatc catttagaaa agctttctga attaaaagcg attgcctttg
     9961 ataaaacagg aacgctcact aaaggaaaac cagttgtaac tgacagcttt ttctttgatg
    10021 aaaaggattt ggcacaacaa cttcttgttg ctatggaaca aaaaacgacg catccacttg
    10081 ctcatgcgat tttgcaatat ttcgattgca caatacctga agaaatccaa tacttaccaa
    10141 tagaagaaat tactggtttt ggattacaaa ctacctattt aggtgcgcaa tggaaagttg
    10201 gcaagcacgc atatgatcct gagacgatga taatcagcaa agagattgcg gaaatgatcg
    10261 aacgtttgga aaatcaaggc aaaaccgtga tttatctttc taaagatcag cagttaattg
    10321 ctgttttagg tctgctagat attccaaaag caaataccca acaagtaatt tcctacttta
    10381 aatcccaaaa catccataca agtatgataa ccggcgacca ttctggcaca gcaaaggcca
    10441 ttgctgaaca agtagggatt gaacattact atgcaagctg tactccggaa gaaaaaacac
    10501 aactagtcaa aaaagagaat gaactttacc aagtaaatgc gatggttggt gatggagtca
    10561 atgatgcgcc tgctttagca gccgcttctt taggtgtcgc tatggggcaa gggacagatg
    10621 ccgctatgga tatcgcagac atcgtcttaa tgaaaagcga cttaaataaa tttgtctaca
    10681 gtcatcggct atctttaaaa atgcggcgga tcatcaaaca aaatattatt ttttcgattc
    10741 ttgttattct cttgttgatt gcttctaact tcctgcaatt tttaaatcta cctcttggag
    10801 ttgtaggaca cgaaggaagc acaatattgg ttattctgaa tggcttgcgt ttactaagac
    10861 ctttacctga tctttctgat tcttcaaaaa aatgtaagtc ttgtccacta tacaaagcgc
    10921 ggctgtcttc tgtcaaataa gcaaagtgtt tttttatgat tttgtttatt ttatgaaaaa
    10981 aacagcttat gaataggtct tgaaggatga attcctactt ttgtaacttt ctaaaaacat
    11041 gttatgattg tttcgaagaa agtgaggaat atatttcatg gaaagttggc tttttttagg
    11101 aattatttta cttgtagcaa ttttagctaa aaatcaaagt ctgatgatag cggcagcttt
    11161 tgttttgatt atgaagctca taccagcctc ccgttccttt ttcccttggc ttcaaggcaa
    11221 aggaatcaat attggggtga cgatcatcac agcggctatt ctaatcccta tcgcaacagg
    11281 agaaatcggt ctacgtgatc tattagaagc ctttaaatcc cctattggct gggtagctgt
    11341 tttttgcggt gggctagtgg ctgtcttatc ttccaagggt gtaggattga ttgcttctga
    11401 tccacaaatc actgttgcac ttgttttcgg cacgatttta ggtgttgtct tcttaagagg
    11461 aatcgccgcc ggaccggtca ttgcttctgg tatcacttac tgtatcttac aagtatttgc
    11521 tttaggaaaa tagaacaaat tttgtctatt ttaggagatt cttaacaccc ccaatttact
    11581 gatccgtgat atactgacaa cgaggtgata caatgttcga aaaattaaag tatttcatag
    11641 gaacaatcgg ctggtttctt ctagcggtac ttttgattct gggcttagct tatctattat
    11701 ttcctgattt gatacaaaat gtgtttgata ctgctttttg attgacctat taaaacgata
    11761 taggggatgc tcctgcgatg caggggcttt ttttaatcaa tgttttgtaa taatttaggc
    11821 cgtgccaatg agacttccta tagaattcga tgctcttttt tctttttggc tcttctgctc
    11881 ttttttgtta tactagatta cagtggacaa aagaaagaat gatcgcatcc acttcaacga
    11941 atatcaaaat ggaagaatta gtgcctgttt tcaggttact ttgaaaagaa aggaagacgc
    12001 ttgtggaaac ttttgaaggt cttttaattg atacgtacgt tttacaagaa cagccaatga
    12061 tgctgactgg gatcaacttg cagcttgagg accgtgtgat caatggcctg cttaaagatc
    12121 gctatttggc aaaatatatc ttacgtttgc ccctaaataa atatgtgctg gaagtaagtg
    12181 gcaaatggaa ccgtcggaac cagttggtca taaaaaaaat gttgataaaa aatatcgatg
    12241 aatacattaa aataatcggt acaccagaat agcctttact gatcaaagga tactggttat
    12301 aaaataactg cagtttctta atagaaaaac aaagaacaac agggaatcgt atcccttttc
    12361 aaaaaaaact ttgcaaacgg ttctctttgt tatataataa cttagtacca aacattccca
    12421 ttcaaacttt gtttaaatag ataaataaaa atctagtttg gtttgacgct attgcctccc
    12481 acaatagcgt ctttttgatt tttttcagct tgtactatcc gtttttactg aaaaaaaatc
    12541 ctaataaaag actgttaagg aaaaaaactt gtcatgttca tattgcaaag cacgctgcct
    12601 ttaggtataa taatgaatgt tgaattatgc ggtaacacgc gtaagaactt aagttatcgg
    12661 aggtgaaact aatgccaaac attgaatctg caatcaaacg tgttcgtaca agcgaaaacg
    12721 ctaatgtgaa aaattcatct caaacaagcg ctatgcgtac tgcaatcaaa aaatttgaag
    12781 atgctgtagc ttcaggtgct gacaacgtag atgcgttata taaagaagct gttaaagcta
    12841 ttgatatggc tgaatcaaaa ggtctaatcc acaaaaacaa agctaaccgc gacaaatctc
    12901 gtttaagcaa aaaaatcgct aaataaaaga atcggcttcg gccgattttt ttatttgtca
    12961 aaaaaaggac aaaaacaaac gaccttcccg catgctttgc cctcgtcttt cttcttatcg
    13021 atttcttaac aactgccctg tgcgcaaaac gaatagttga aaacttaatt ctttttcgat
    13081 ttgtcccgtt ttgatttgat aatccatctc aactaattga tcaaacaact gtaccagcaa
    13141 atgttcatcg aattttttta cttcctgcat ggcaagtttt acacgatatg gatggatttt
    13201 caatgtttca gcaatatttg cctgctgata accaatcttt acaagaaatt ttgtctgtaa
    13261 aaataaacgt aattgagaaa gcaaaatcgc attaattttg attgtttctt ccccttgcat
    13321 aactaaatct tcgtataaac gcaaagcttg ttccgtcttc ccttttaaaa tatattgggt
    13381 catgtcaaaa atattatgtt ctaacgtttt aggaactaat tcttctactt ctcttaccgt
    13441 gatttgctgt tgattttgtc cataaaggac caatttctgt aactctctca tcattttgga
    13501 taaatctaag tcggtcaacc tcaagaataa atcaattgca cttcgttcca ttttgattga
    13561 ggaattctct aaggtattca tcagatattg acgtacttct ttttcattca actgttggac
    13621 attgatgata attgctgttt ttttcaattg tttcgttact tttttccgtt catccaactt
    13681 ttcatatggc gcaaagaata ctaatacagt cgattctaat ggttctttca aataagccgt
    13741 caataaatca gtattttgtt cgattccatt gtttactttt tcacctgtta aaaaataagg
    13801 attctctacg aatactaacc gttgatctcc gaaaaacggc aacgtctctg cttcagctac
    13861 tacctcatct aaacttgcat tttccatgtc aaaacttaaa aaatttaagt catcttcgcc
    13921 ctctaattgg atttttttga caatttctgt ccgaacttga tcttggagaa acttttctgt
    13981 tcctaaaatc aagtaacaag atgcaagttt ttctttacgt attttttgta actctgcttg
    14041 tatactcaaa ttactcacca ctttcaaaaa ttatcctacc atgagcagat gagaattact
    14101 agtctatcat tgtttccact agcccatgag gatgaaaaac tggatgccag cgataggtga
    14161 tcattccttg ttgatccgtt cgccatgttt ttatctgatt ttcttctagt gtctccaata
    14221 cttcgctgtg cgggtgacca taacgattat ttacgccgca ggatataatc ccatgttttg
    14281 cttcaatatg ggaaagaaaa tctgtgttac ttgacgtacg gctaccgtga tgacctaatt
    14341 tgactacatc ggctttcaga ttaggataaa tcaaaagcaa ttgtttttcc attgcttgat
    14401 tcaaatctcc taaaaataaa aagcgcctat tgttatattc catccataga ccaatcgaat
    14461 cttcatttct tccctctccc ttttcaaaag gagtcaatac ataaagatca taataaccgc
    14521 tgatcctgtc cccatgtttg atttcaatga tctcagtacc atttttttga gcttcagata
    14581 aatgcctttg taatggctca tttcgtccag cgccccaacc gatataaatt tttttgatag
    14641 taaaatgctg agataacgtg ggtaattctc ccacatgatc cgtatcgtcg tgtgtcaaga
    14701 tgagtttatc gattacactt atcccctgac cttttagata tggaacgata tttctttcag
    14761 caggaggacg tatcatacct tgcgcccact tttctctttc aaagtgtaac tgacctccag
    14821 tatcgatcaa aatcgtttct tttcgaaaag gactttgcaa aaagatactg tctccttgac
    14881 cgacatcgat aaaagcgatc gttgaagaaa agcgtaaaaa tggcaaagtc aaaaaaataa
    14941 aagaaaaaag accaacataa aacaagtgtt tcacaggttg ttctttcaac ctatccatac
    15001 aaatcagtaa tccgaaaata tacatcccag atagcacaag gggcaaacgt cctataatga
    15061 gagacgaccc tgaagaaaaa tcgattattc gctcaaataa gattaatccc tcttctatca
    15121 gcaaaagtaa agtttccggc agccagcgat tgaagagtgt gagaaataac acaccaggaa
    15181 gaaaaagaaa cttgaatacc ggtgcaatca gtaatgtaaa aagactacct aaaataggcc
    15241 actcataaaa atgccataca atcaacggca tcgtgatcat agtaaacagg acgtgagagg
    15301 ccgttttttt caaccaatct ccatcaaaag aaatcaggat aaacaaaaaa ctcatgaaaa
    15361 aactaagctg tccacccgca tgaaaaagaa atcgaggatc aaatagtaaa agaagccaaa
    15421 agacaactgc aaataagtcc atggtggaat aatgcttatt cagcaatttg actagcaatc
    15481 gcaataaaaa cagcaaacta gcacgcaaaa cagaaataga acttcctgca atagaaacat
    15541 aacagatagt tagtagactc aacggaataa tactgacgtg tagtggccat ccgatacgtc
    15601 gaaataaata atacagccac cctaaataaa attggatgtg tagacccgat agactgaaca
    15661 gatgcagcaa accactctcc ctaaaaattt cttctggttc tctgaatcct gcatctttat
    15721 agccccataa taaagaatta atatatacac ttgttttttg cggaaattca gattggacaa
    15781 aggagataaa agatcctctt tttctctgaa gccagtttcg ccagtgattt tgcatggtga
    15841 tctgttcaat cttttgaatt tggaaccgtc cagaaattct atggttcctg aagtaagctt
    15901 cttggtcaaa attataaaga ttccgctttt ttgctggaga gatgaactcg ccttcagcag
    15961 tcaattctat tccctttcct cggaagttaa gccatgtttt caattctgct tctgtgttta
    16021 cttgataaga aagctggagc ttttgtttat tctctgataa ccttccttcc attgtcaaaa
    16081 aattaccatt tgcacgaatc gtatctgtaa atacagtaat ttgcgcagat gttgtttttt
    16141 cctctaaaaa tgaatactct tgtagcctat aagaaatgga taccgttatg ctacacaaaa
    16201 tacacaagta cgcgataaaa cgtccttctt ttagcgaaaa ccataaaaaa attccggcaa
    16261 gaagcaaacg ataaccccaa ttgtcagtgt aatacaacaa acagataatc atcgacgtaa
    16321 ttgccggaaa aatcaaccga tttcgaacat gcctttctaa aacgctagac atgttttcct
    16381 gttaattcat tttgaagttt tgaaaaatgc tgtgagtcta aagcaacttg gtgtacagat
    16441 acacctaatt gttcgatcaa cgcttgggca taaggatcat ttcgatagtc atggagataa
    16501 ttgatccctt tcacaccagc ttgcaacaaa gcctttgtac aggccaaaca tggaaaatgt
    16561 gtgacataga tttctgaacc ttccgtcggg ataccaagtt ttgcacactg taggagtgca
    16621 ttcatctctg catggatcgt tcgcaagcag tgtccatcca cgacgtaaca cccttcatct
    16681 atgcagtgga catctccact gactgctcca ttatagccgc cagcaatgat ccttttttct
    16741 cgaacgagtg tcgctcctac ttctaaacgt gtacacgtac tacgtaaaga taacagtact
    16801 gcctgagcca taaagtactg gtcccaagga atgcgttcat tatgtgactt atcagtaact
    16861 ttttctttat tcgtatcgca tgcttttgaa gatttttcta acatgtttta tattcctccg
    16921 ttttttcaag ttgattaaaa agtactcatt gcttaaacaa aatattcttt aaatataacg
    16981 aatcatcctg tctgacaaaa gatcctaccc ttcactgccg aaatgaagtc cgtcatccat
    17041 tagcccatta taaaaattct tcgaatccat ttgttcaaga aattccgtat aaaaatcgat
    17101 acttcacaat catggcgttt tgctacttct gtgagaacag agatatattc tttgatcaac
    17161 ttgttattcc gatatttttg ttttgtctca tcgactgctg gtggcgtgat aaagaaagca
    17221 tgatcagaac tatagtcttt tttcagctga actatcatcc actgcagatt ttttggtacg
    17281 tttctttaga aagttgttta tgctctgcta gatcattcgt cccaatgaga ataaatactt
    17341 tatctgctgt atagctaagt acatctttgg caaagcgttc tcgtagagct tgactgttat
    17401 tgccgctgac agccgtattc ataatatgaa tcccagatat tttattttgg agatagacat
    17461 tcagcattgg tttttttaat gtttctttgc gaacaatcaa gctatttcct atccaaaaat
    17521 actacctcct cgtcctgcca agctcttaga attggattat tcttagtatc cattagcgtc
    17581 catttattgc tcgtgccagt cgattttgaa tcattctatt taattgtaac gaagtttttt
    17641 agtttttcaa ctgtcttttc accgatacca gaaatctcct gcaggtcttc cactttctgg
    17701 aaggatccgt tttcttctct aaatcgaatg atttcttgcg ctttgactga tccgatccca
    17761 ggaatctgct gaagttctga tggagtggct gtattgatat ttattttggg aggctcggaa
    17821 tctgctgtag gctgttttcc ttccgaggat gccaactgat caagatgttc tggcagctct
    17881 tcacctacag cagggatata taggacttgc tgatcttcta tcttcgcaga aaagttgatt
    17941 cgatcgctat ccgcttcttc taataatcca cctgcacgct ttagtacatc atagacccgt
    18001 tcttctgaag aaaaggaata gataccaggc tccttcacac ttcctttgat atcggtgtac
    18061 atgacctttg gacgagcatc ctcttctaca tcgtttcctt cagctgatgg atcttctcca
    18121 ctggaactaa acgaagaaag tatttctttt gcagcagtct cttctgtttc tctataaaat
    18181 agcaaatttg ataataaaaa accacttgcg atacaaaaga taagaactat catagacatc
    18241 caaatgatca atttgggtga agctagtatt ttcttgctga ttttttctat cattgttctt
    18301 ttttcaaaga atgaccagta aaaatcattc attactcctt tcatttaaaa ttgaagaaac
    18361 agtgagctct ggcaaaatcg tcccttcaac atctaattac gtcaattttt tcttttgttt
    18421 ttatttttat cgtgaag
//
LOCUS       DACDYM010000051        17977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDYM010000051 DACDYM010000000
VERSION     DACDYM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.051"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            97..2424
                     /locus_tag="IWM59_001811"
     CDS             97..2424
                     /locus_tag="IWM59_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9979841.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2550..2879
                     /locus_tag="IWM59_001812"
     CDS             2550..2879
                     /locus_tag="IWM59_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9979842.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2919..3923
                     /locus_tag="IWM59_001813"
     CDS             2919..3923
                     /locus_tag="IWM59_001813"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9979843.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3920..5349
                     /locus_tag="IWM59_001814"
                     /pseudo
     CDS             3920..5349
                     /locus_tag="IWM59_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5555..6728
                     /locus_tag="IWM59_001815"
                     /pseudo
     CDS             5555..6728
                     /locus_tag="IWM59_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            complement(6890..7345)
                     /locus_tag="IWM59_001816"
     CDS             complement(6890..7345)
                     /locus_tag="IWM59_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979844.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7934..8356
                     /locus_tag="IWM59_001817"
     CDS             7934..8356
                     /locus_tag="IWM59_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9979845.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8522..9715)
                     /locus_tag="IWM59_001818"
     CDS             complement(8522..9715)
                     /locus_tag="IWM59_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9979846.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9939..10316
                     /locus_tag="IWM59_001819"
     CDS             9939..10316
                     /locus_tag="IWM59_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9979847.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10304..10642
                     /locus_tag="IWM59_001820"
     CDS             10304..10642
                     /locus_tag="IWM59_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9979848.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10776..11219
                     /locus_tag="IWM59_001821"
     CDS             10776..11219
                     /locus_tag="IWM59_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9979849.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11481..12296
                     /locus_tag="IWM59_001822"
     CDS             11481..12296
                     /locus_tag="IWM59_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9979850.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12306..12968
                     /locus_tag="IWM59_001823"
     CDS             12306..12968
                     /locus_tag="IWM59_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9979851.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13173..14837
                     /gene="alsS"
                     /locus_tag="IWM59_001824"
     CDS             13173..14837
                     /gene="alsS"
                     /locus_tag="IWM59_001824"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9979852.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14850..15560
                     /gene="budA"
                     /locus_tag="IWM59_001825"
     CDS             14850..15560
                     /gene="budA"
                     /locus_tag="IWM59_001825"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9979853.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15690..16187
                     /locus_tag="IWM59_001826"
     CDS             15690..16187
                     /locus_tag="IWM59_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9979854.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16372..16632
                     /locus_tag="IWM59_001827"
     CDS             16372..16632
                     /locus_tag="IWM59_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979855.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16994..17233)
                     /locus_tag="IWM59_001828"
     CDS             complement(16994..17233)
                     /locus_tag="IWM59_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979856.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            17519..17833
                     /locus_tag="IWM59_001829"
     CDS             17519..17833
                     /locus_tag="IWM59_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979857.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         6094 a         3103 c         3540 g         5240 t
ORIGIN      
        1 aaagccccaa actttctata catcaataaa ataataaaaa ttaaatttag aataacgtgt
       61 aaaggattat ttgatgattt tttattgaga ggagggatga aaatgattaa gaaaactcaa
      121 aataaaatcc aacgtatatg tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa
      181 agaaaatact tacatcccat tatgatttcg atcatcttga tttttacttt cttagggagc
      241 ttatcaagta tttatgtaaa cgtttataca gaaacgtcaa attttgctaa ccaaacgaca
      301 gaggagacaa atagtccacc ctattctgaa atacagtcca tagaaagcac caatcagtct
      361 agcaacaacg agacaacctc aaccacatcc tcgactcaac tgattgatcc gacagaaaaa
      421 gaaatcacag cagcaaaagc agcaggggag aaagcttatt cagaaacagg tagaagccag
      481 acatttagta ctgttgcggc cactgcgaat ttcaacggac ataccatacc agatggatat
      541 acagtgatat atgccaaaga tggagtcatt gttgcggtta acggatcgat gagtgcggat
      601 aaaactgcgt tacaaggcgt aaccaaagca atcgctttta ttgataaccg tccagatatc
      661 cataatctgt ttattggctc cacagtaaat ggtactacta tcacattccc aaaaagtatc
      721 tatatggggg tggctaccgc tggaaatacg atcgattcag ttacacccaa tgcggataaa
      781 agttattctt ttacaccctc aacatgggga gggcttcctg ccggaggagg tatgagtact
      841 ttttcaatcg gtatacagtt agcaaatgat attccattca cggggtactc gatcaaaaat
      901 gggcagatcg ttgatggtaa gggaaatgtt tcaaattttc caacctatca agcagcaacg
      961 gttcaggtca atgcgaatcg gacaggaact ttttcttata cagtcaatgg tggagcagca
     1021 aaaatcggaa cgtacgggac tacattatct ggaatccatg tgggtgacaa agtggttatc
     1081 actccaacag cagtaacggg ctattctttt gcacaaacaa acggttcttt cacagcccta
     1141 gaaagtaacg tcgatacggt gacctataca ggcaaacaaa caccaatcac tattaagtat
     1201 caagactatt ttggacagat gattgctcct tctaaaataa tgaatcttac ttacggaagt
     1261 gctgcacaag acctgacgac taatacgcct actatcaacg gatatacctt tacagctgtt
     1321 tcagcgacag aaacaaagaa ccaatcagca atgagtgttt tagctagttt agatacaaat
     1381 ggaaatgtaa tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat cctatattat
     1441 aagacaaatg caacagtatc aatcaatgca aatggcagta agtactatga tggtctttct
     1501 gttgtaccga tcgttaaata tgcctttaat gataaaacag aatcaacatc ccttttaaac
     1561 aatgatctca gtattgatca aatcacatgg aatccaactg atttcaaagc gatgaatgaa
     1621 aagggagttg aagtaactgc gcctactgaa attggaacct atacgtcttg gcaattgaca
     1681 aaaagcggac tagctaaact agctgccaga acaaactatt tatttactat cgtgcagaca
     1741 tctgatgtct atacgattaa gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca
     1801 tatgatggaa aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg tgtcactagt
     1861 tcgcttactc cagtacaagt ggcacttagc agtatggatt acacagcaga tgcacaatca
     1921 gcaaaaaata tggttaatgc tggcagctat acaattaaac taacgaatag cggaatagac
     1981 aaagtcaaag cagcaaatcc caagttgtca tttactgata tagccaacac atcaggaact
     2041 tataccatct ataaagctaa cgcagtgatt actgtcgatg acgctgcctt taattatgat
     2101 gcccaaagtc actcgatttc ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa
     2161 tcattggatt ataccctaac agttaacagc cggacagata ttggatcaca aatagtaacg
     2221 attagtttag gcgaaggaag tgtaaacaat aattatacga ttatcgcaac agataccgct
     2281 aagttgacga tcaatccgac tctcttgctt ccttcgacag gtgggatagg tctactgccc
     2341 tttgtctttt taggactgat ctttattttt tctggcttct tttattttat ccatcgtaag
     2401 aaaaaagcag gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg
     2461 ttctcttttt tatcctactg gtctttttta taggttctcc gactgtgtca gctagagaat
     2521 cacctattac atttactttg cataatcgaa tgttcgaaag cgataagatg ccaagtatca
     2581 gtcaaaacga cggaataaat atcccggata actttcaagg agtaaaaggg ttgaacggcg
     2641 ttactttcgc tatatatgat gtctcggatg aattttacaa gctgcggtca gaaggaagta
     2701 gtgtggaaga cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa atactggcag
     2761 aaaatgtgac aaaaacagta gacgaggaag agggcattgc ttcttttagt gcatcggaca
     2821 aagatgagca aggtcgtgac gctgtttacc gatttgcgga aataaagact tcagattaag
     2881 taaaagaaaa atcagcacca tttgtcgttg tgcttcctgt gacagattca tctaatcata
     2941 aattgacgac catccacttg tatccaaaga gcgaacaaaa aatacctgct gcactcacat
     3001 taacaaaaac agtagagaat aagcagacag attttgcaga tggggataaa gttccttatc
     3061 tcatcaccgt aacaattcct gagaacatca acgagatagg aacctatacg atcaaagata
     3121 cagctgatcc tcagttatgg ctagaaccag aaacgatcga ttttttgatt ggtggagaca
     3181 agattcatac atttcacaca caaaaaacaa aacatggttt tgtcttatca gattctggaa
     3241 aagatcttag ttcgtttgct gggaaaaaat taacgatccg ttaccaaatg acattgaaag
     3301 aaaacagtga aaaaacgaca tttgataacg atgtacgttt gacgactgac aatcaaacag
     3361 tagaaacaca tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa
     3421 cgaaacaaaa actagcagat gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc
     3481 aagaaaacgg gataaatcat tgggtagcca aagatgattc aagagcaacg atttttactt
     3541 ccagtaaaga tggaacattt tctgtccatg gattgtcttt tggaagttac gaactagtgg
     3601 agattaaacc gcctaaagga tatatattga gtcaatcaac cattctcttt gaagttgaaa
     3661 aaggcagtta tgatccgctt catcctatac cattggaaat catcaacgaa ccaaaagcaa
     3721 atccgacgaa taatggaaaa acgccagttc agatgcagac aaatggtcca tcaaatgaaa
     3781 aaaatggagg aagctttcca aaaacaagcg aagagatgct tggaggtttc tcaattttag
     3841 ggttgttgct agtcctttca acagggacag cttggtttta taaaaaacaa caaaaaggaa
     3901 acaataggag ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt ttttaggaac
     3961 tgctatcatg ctgcttcctt tcgttctcgg cctaggaact gcagaggttt ctaggccgta
     4021 tcacctacac cagaaaatgt tacggttaat ttgcataaat tgaaattcac ttcagcacca
     4081 gaaaatcaaa taaacaacgg aacggaattg acatttccga atagcgaacc gttgaacggc
     4141 gttgaattca acgtatatga catcactgct acgtactatc caagtaaaga cacagcagtt
     4201 ccagcagacg ctacaccctt cgcaagtgta acgacttctg gtgaaggatt agctaacctg
     4261 actcttccag gtaaatctga tgggaaagat gctgtatacg tctttgtaga aacaccaaag
     4321 cccggtgtcg aaacgtcgcc aaatatcgtg ttaagcttac ccttttataa cccgaattca
     4381 tcagaccctg acaaaccttt agacacgatt cacctatatg ctaaaaatgt catcaaagaa
     4441 agcaatatca aaatccagaa agctgggaac ttttcagggg tagataagtt agcaggggcg
     4501 caattccaga tcaaaaattc agagaatcaa ttcgtgaccg gttttgatga aaacactggt
     4561 gtcgctctct atgatcctgc tgagtcagcg ttgacattca caactgatac tgaaggatcc
     4621 atcagtgtca atggattaat agacggcaat tatactttag ttgaaacaaa agcaccagat
     4681 ggttatcaat taaccaatcc acaaaaagaa actactttta tagtaaaaaa cggaaaattg
     4741 caacaaacaa ccggtatcat ccatggcgca gatgaagcca atcttgggaa tccaaacggt
     4801 gtagtcaatc gggaaaatac aactacggat aacacgatta ctgtagaaaa tgtccaaaac
     4861 tacggtgatt tccacttcat caaacaagat gtgaacacgc aagaaaaatt agccgatgca
     4921 gaattttcta ttctaagagc aaaagatcca actgttaaat taatgaaaaa aatagcaaat
     4981 cttgcctcag gagaataccc atatgcttgg tcagatgaag tcacaggttc tgactgggaa
     5041 gaagtgaaat tgatttctga ttcagaaggg aaattttcaa tcgaagggct gaaacaaggg
     5101 gcatactgcc ttcaagaaac aaaagcacca gaaggctacg cattacctaa gaatgaagct
     5161 gcgtacactg aatttacagt agatgatgat ccaacaactt ctgatgaaac aacgatcgat
     5221 aaccaaccta aaggaatctt gccttcaact ggtggtatgg ggatcatcgt attcatcttg
     5281 gtaggtactg ctttagttgg aggagcagtg atctacttca aaaaacgcca tcaagaaaca
     5341 gaagcttaaa ctattggaaa caaaaatcat acgataaata aacgatagtg attcaaatgg
     5401 ggagaaaggt tgtgacaagt ggtcggtctc ttggtattcg ctcaacaaac ggcaaaatag
     5461 gaggaactat tttgccgttt gttgagcgaa tactcaggtc aaggcgctga tttcacaacc
     5521 tcttcatata catattagaa aataggtgga catgatgaat aaaagcagaa taagaatact
     5581 ggacatcttc atggcaatca ttttagtcgt tgggatcggg atcttttctt atccttttgt
     5641 agaagattca ctgaatgatt ttcttgccca gcaaatgatc atccattatc aaaagcaagc
     5701 atcgaataag aattcagagg aaataaaaaa aacagcagga aaaaatgaca aagaaaaacc
     5761 aacagttggc agagcaaaac gtatcaccag ggataggcag tttcaaccaa gcagttgatg
     5821 caaaagcatt aaaagattta cctagtaatg ccttttttat ggaacatatg cttggagtca
     5881 tcgaaatccc aaaaatcaat gtcagcttac ctatattcga tcagacaacg gaaatatttt
     5941 tgcaaaaagg aacgtcatta ttggaagaaa gttcttatcc gactggcggg aaaaacaccc
     6001 atgccgttct ttctgggcat cgtggactcc cagaagcaaa attatttaca gatttgccta
     6061 agttgaaaaa aggagaccag tttttcatcc agatcaatgg aaagacattg gcgtatcaag
     6121 tagaaaaaat ccaagtggtc ttacctgatg aagtagattc tttgggtatc caaaaaggca
     6181 gagatctcgt gactttgctt acctgtactc cttatatggt caatacacat cgactgcttg
     6241 tgacggggca tcgtattcct tatcaagcaa aagaggcaaa aaaagcgatc caaggaattg
     6301 atcaatggaa aaaatggaaa ttttttattt ggtttatcgg tatactactt ggatcaatcg
     6361 gcttagtttg gctactcatc gcttatttag attctttggc catcgctaag cgaaactatc
     6421 ctttatcttt ttatgtaaag aacacaaacg gacgaccaat cgaaggaatg gtcttttctg
     6481 taaaaacgct caacggcaaa cactatatta ctagagaaaa agttccattt gtgaaagctt
     6541 ctgatgaata cggactagtg agattttctg atttaaaagg caggaactat cgcttgcaac
     6601 atgaggagct cctgcttaaa atccatgtaa aacataaaca ttcgaaacag ttttcaatga
     6661 aattgaaaaa aggaagatat aaactgcgaa aagaaaaaga ggtgtactat ttgattgaga
     6721 aagaatgaat gaagatgtcc aaaaaagcag agggaacaga ctaaatgaaa aaagagagaa
     6781 gctgacgaaa tggacgtgta aactgctatt tttggtttac tatctggaac gccagcttct
     6841 atttttttat gtttcagaag aattttatta ttatagtata acttttgaat tagtttccat
     6901 aacatttgga gcaaggggtt aaaccacgag cttgagcttc gcttaaagtg gcaggggtat
     6961 acgtaccatt tccacattta tgtgtatggt attttgaacc ggtcggtgtg acatagacag
     7021 tttgttcatt attttgctca ggagctgcgg cttgtgcctg ctgagaggcc tgttcagctg
     7081 ctgcttgctg tcgcgcctgt tctgcttgtt gctgtgcgat tgcttcttgt tgcgcttgtt
     7141 ctgcttgttg tcgtgcaatt tcttcttgct ttttccgttc ttcttctgct tcttttgctt
     7201 tgattgtgga atcaattgca gcaagacggc ttgtcaactc ttgtttgttt tcaggtagcg
     7261 cttggatagc tgctgaagca gttgcatagt tttcttttgt aggatttgct tcagcttgtg
     7321 acaaagcaga ttccgcttct gtcacttttt tttctagtgc agctttttcc gctttttgtt
     7381 tctcttcttc ttttttcttt tcaatttgtt cttgttcttt cgatgaatct gccgtacttt
     7441 cggagatcgt ggtactgctg ctggaagcct gagctgaact tgtgctttga gtagtagacg
     7501 tagtagcacc acacccactc aacaaacttc ctgcgaccaa aaacaatgca actaattttt
     7561 tcactaaatt tcctccttaa gtaaaaaaca accgttttca ataaatgtcc ttttcaattg
     7621 tttacttact tgttataata attgtgttat gaatagtgat tgaggaaact taatctaatc
     7681 ccgttttgat acacgggatt ttttgctata gttctgttat taagtatgca ataataaaat
     7741 atgtacgtta ttttattatt agattattga catttatata ataaacgatt aaatgattca
     7801 tatcaataga atacttagga aaaaaagaga aataataaca ggtttatgca ctattgacgg
     7861 tgtgacaata tgatatttgt atccgttttt ttcttgtatg tagtatagta aagtagatac
     7921 aggaggagaa aatatgcaga cttttttaac aaaagtttct caaaagaaaa ttaaaatgat
     7981 tcatttactg cttgaaacag aacgttggtg ttcaatagaa gaactacaaa acgaattgaa
     8041 ggtatcaagt aagtctatct tgaattatct aacagattta gaagaattgt tccaaaaata
     8101 tcctgataag gtcattttga agaatgagaa taatcggcgc ttttctatgg aaaagcagga
     8161 gaacttccca atctacatta tttatcttca tttctacaga aaatcatata attatcattt
     8221 gattgaattc atgtatcaac atcctgagaa aaatttggaa gattatgcag acgctcaata
     8281 tacgagtgta tccacagttt ttcgttatgc taagttatta gtaaagtact ttgaaatgac
     8341 ggacgtcaaa gaatagtagc cgccagatag aaggaaatta gcagttgaaa aagctgccct
     8401 gcatcaagta atacgaccag ccaaacggaa acagtgtctc atgtttttga tttggctggt
     8461 cttattaccg cagatgcaag cttttgccac acctctttct tgtcatttac agactcaact
     8521 atcaatgctg caagctttcc cttaatgctg taacaccagt aagagttgtc aagaccagca
     8581 gactgactag gtaaataccg ctcaaaaaca agattacggc agttaaacta taatgatcca
     8641 tcaagaaacc gatgacgata gaagaaaaac caccaacagc tcgtccaagg ttcataatga
     8701 aattattcgc cgtcgcgcgg atttctgttg gatacaaact actaatcact gcgccatatc
     8761 caccatacat gccattgata aagtagccga ctatcacagc ggctgctacc aaactccact
     8821 ggctatgggc taacacaagt agatagacag ataaggatga acaaattaaa aacaaagaaa
     8881 aggctagttt aggccccaat ttatccatga ttatgccaaa agtcatcatc cccaaagaca
     8941 tgcctaaaat agtactgacc atccacaatg aagacccaga aaccgaaagc cctaatcgat
     9001 tttgcataat tgaaggcagc caattcatta acccaaaata gccggcaatt tggacagtta
     9061 ccatgatact aaggccacat gtttgccaag caagatgttt tgattcaaac agtctgctaa
     9121 gtttggtttt cttttcggca gattgtacag atttgaattc ctttggttca gtcaagccgc
     9181 gacgaatgaa caagaccatc aacaccggca gtacacctac gacatacaac atcttccaac
     9241 caaacgttgg aatgatcaca gcagctaaaa tcgcagccaa agccgcaccg acctgcgcac
     9301 caataccagc aaccgaagaa gctcgtccaa tttgtttttt tgaaaaactt tcggaaatca
     9361 aggacataca cgccccgtat tctcctcccg ctcctacacc tgcgataaag cgaaagagat
     9421 aaaccaagta gatgtttgaa gcaaaataca tcaataaaga agcaatcgaa aacagaagga
     9481 ccgtttgaga aaatactttc acacgtccat atttatcagc tatgatgcca aaaaaaatac
     9541 ctccaatgag catccccaaa ttagtgatcg ttgaaatgaa acccgcttgc gtaccactaa
     9601 tatgaaaaga agtaatcatt gaagataatg aaaaagctaa aaacataata tccatattct
     9661 ctaaagcaat tcccgccgat gtcgataata ataactttcg ctgatacgtt gtcattttta
     9721 tacccctttt cctaaaattt ccctaaattg ttaaaagtat atccaaaaaa gaataaaaaa
     9781 acaagacttt tttatttgaa ataatacaca aacaaccgat atagataatc cagcttagtt
     9841 ttaatagaaa tgagtattga cttggagtac acttaaagtt gtagaataaa taaaatacat
     9901 aagtaaagtg gcaagaaatt aagagaaaga gtgatcagat gaatatcaaa caagtcagtg
     9961 aagaaaaggg catttcggcc gatacattgc gctattatga acgaatcggg ttgattccac
    10021 cagttaatcg gacaaaaggc ggaatccgtg attatacaga agaagaccta aaatgggtcg
    10081 actttacgat ctgtatgcga aatgccggat tatcgatcga gtctttgatt gaatatattc
    10141 gcctatctag cgaaggagaa gcaactgtat ttgagcggcg taagcttttg attgaagaaa
    10201 gtgaactttt gacgaaaaaa attgctgaaa tgcaagaatg tttggaacgt ttacaaggaa
    10261 aaatcaagaa atatgatcgt gtgttatcta gaaataaaat ggaatgtatc aagtaaaaaa
    10321 atcaaggagc atcgctatgg agttacgctt actaaaatat ttttggacaa ttgcagaaga
    10381 aggaacaatc tctcaagccg cagaagttct acatcttaca caaccaacct taagccgaca
    10441 actgaaggag ttagaagagg aactaggaac agaactgttt gtacgcgaaa accgcaaaat
    10501 gatcttgaca gaagcaggtt attttctaaa aaagccgagc agaagaaatt ttagatttaa
    10561 cgaatcaaac cactcaagaa ttcgagaaca gaaaaaaaca attattcagc ggccgattta
    10621 cgatcggctg tgtggaagct gataattcga atacattggc actattacta gaaaagttga
    10681 tccgtgatta tcccagcgtt caatttcata tccacagcgg aacaagtgat gatatcacgg
    10741 agtgactaga aaaaagcctg ttagatttag cgattttgct agatccagta gatacagaga
    10801 aatacgaaac gattttgtta ccaagaaagg aaaaatgggg attgctcatt ttagaaaatt
    10861 ctgttttagc aaaaaaagac gtgataacaa aagaagactt tgcgaatgta cctcttttga
    10921 tctcaaagcg tccagaaata cagcgaatgt ttgctgactg gttaggcata aaaaaggagc
    10981 agttgctgat cacaggaacg tacaatttga ttttcaatgt atttgcgcta attgagaatc
    11041 aggtaggtgc tgctttagtc atagaaggtg ctgtaagtca tctttcaaag cgagtgacat
    11101 tcgttccgct tgacccaatg attgaaacga actgcatgct agtctggaaa aagaatcgga
    11161 ttttatcacc tgtagtcaat gaattgattc accgtttcaa agaagctttt gaacagtaat
    11221 aagtatctct aatcgaagaa atttcaatac aaaagcgaga cgtatgacag tcttgctttt
    11281 gtattttttc atttttaaat caaaaaaaat aaagagattt acataacttg ttaaataagc
    11341 gcttacaaac ataagaagaa cggaaatgaa tcagctataa taaccgttgt tctggattgt
    11401 aatcattaca aagaaatgaa gattataact ttggaggtgt tcgaaatgaa agaaaaaaag
    11461 caaaaattcg ttcaaaaaac atgcccttta ttcaatggtt gggtatgatt ctgctgattg
    11521 tattagcttt attgacgatc attccttttt tatggatggt ttctgcttca ttcaaagcaa
    11581 ataatgaagt gttcacgatt ccgattcaat ggatccctaa aacttggcat ttagagaatt
    11641 atctggttat ttggtctcgt attcctttag tgacattttt taagaataca gcttttttaa
    11701 gcgttgtgat cacgttgatt cagttgttta cttctagttt tgctgcttac gggttttcta
    11761 aactaaattt taaagggcgc gatattttat ttttagcata tatcggcacg attgctgtcc
    11821 cttggcagtc ttatatgatc ccgcagttca ttatgatgag aaaagtgggt ttaacatata
    11881 cgttgtggtc gttggtgcta ttacaagcgt tcaatgcttt tggtgtgttc ttgttgaagc
    11941 aatactacag tagtatccca gattctttat gtgagagtgc gcggatcgat ggcttatcag
    12001 agtggggaat ctatcgcaag atcattttac ctcttacaaa accggcgatt gccagtttga
    12061 cgatcatcac attcgttaat acatggaacg attatatggg gccattcatt tatttgtctt
    12121 ctacggaaaa taaaacaatc cagttagggt tgaaaatgtt tattgggcta tttgatgcag
    12181 agtacgcatt aatcatggct gcctcagttg tttctgttct tcctgttgct tttatatttt
    12241 tagcgatgca aaaatatttt gtagaaggga tcgcttcatc aggaatgaag gaataggagg
    12301 tgattatgaa agagcagcgt tttgacaaaa atgtttatat ggaattcttt tattggttgt
    12361 atcaatcatt ggcaagtagc ctttgttttt ggtgtgtcaa tatcttgtat atagcagctg
    12421 cgttcctttt gtcgatagat ggaaggaatg tcttttcctt tattttttct ttacttttta
    12481 ttggtccagg ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt
    12541 tttcaccagt acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt
    12601 attggctatt tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac
    12661 gtttttctta tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt
    12721 cctttagcat caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag
    12781 ttctacggat agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt
    12841 tactattgac aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag
    12901 gtccttctat cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg
    12961 atctgtagac tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact
    13021 gtcattcttt ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac
    13081 ttttgaatga aacaggttta gaaactgtat caattatttg tgattatttc taactaataa
    13141 ttaaaaataa agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga
    13201 aatctgtagt agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg
    13261 caaaaatcga tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc
    13321 gtcacgaaca aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag
    13381 gagtagttat cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag
    13441 cgacagcaga aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat
    13501 tgaagttgac ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca
    13561 gtgcagaaat ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg
    13621 ctaagagttc aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac
    13681 ctgtccaagg gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag
    13741 atgatatccg ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag
    13801 gtatgcgagg atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag
    13861 cattgcctgt tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc
    13921 atttctttgg tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa
    13981 gtgatctagt tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg
    14041 cggaaaaaga tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta
    14101 tgcagcctga aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt
    14161 cattagagcc tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcaa
    14221 aattaacgga gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag
    14281 aagttattaa cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag
    14341 gtagtcatta tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat
    14401 tcagtaatgg tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg
    14461 tgcgtccaaa tacacagatc gtatcagttt ccggcgatgg gggattttta ttctctgccc
    14521 aggatttaga aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg
    14581 gacactacaa tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag
    14641 actttggtcc agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag
    14701 ctacaagtgt agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg
    14761 taattattga tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac
    14821 cagatcaatt ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga
    14881 cattaggagc tttgatggcc ggcttgatgg atggaacaga aacgattgct catatattag
    14941 aaaaaggaac tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag
    15001 atggcgtagc ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac
    15061 ttacccccta tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa
    15121 ctgcagatgg agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat
    15181 ttttggcagt gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc
    15241 aagaaaaacc ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa
    15301 gcaatgtcca aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg
    15361 cagcgggatt tcacctgcat tttatcgatg atacacatac atttggcgga catgtaatgg
    15421 attttgaagt ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt
    15481 ttccagttga tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag
    15541 aaatccaaca agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg
    15601 tatctcgttt ttttgaggag aaaacctagc gtttacaaaa aaactgagct ataatcttct
    15661 ccatagatca aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat
    15721 acgaaatttt attattacag caacaaggat tattagaagc agaagataac tggttagcaa
    15781 atttagcaaa ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat
    15841 attatttatt cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca
    15901 cacgtatcaa gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga
    15961 tcgttgatga tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag
    16021 aaatcgtagt gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg
    16081 gagtaacgaa agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt
    16141 tattagcaaa ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga
    16201 aacaaaaaag aagagaaatc aagaataaaa agagccggaa agacagtggg catttctgtt
    16261 tttcggtttt tttatttttt tataaaatca caagtgttat tgattttcat aatcatattt
    16321 taatgttatt atcattttaa ataatgaaaa aacattaagg atggatgaaa tatgaataac
    16381 gaaactatca ttttaggaaa aaaatatatt tgccaaccta ttggcttgaa acaaaaagtg
    16441 attggagaag taatcagtaa attagaaaat tgtgttgtga tctgcgtaga cgaatatcat
    16501 gacgtcgatc gcaatgaaat attagaaaaa tgcggaaaag tagtggcaaa atatactcac
    16561 gtttatgagt tggaaacaac agaatatcta tataactata gcaacaaata tgaacatact
    16621 ttagtttcat aaagatttaa ataaagtgcc ttcaaaagcg agaagaccaa gcttttgaag
    16681 gcactttatt tagttggcag tgattgttca tgatttttga tttattcaaa aggttcgcct
    16741 agagaatctg tctctgtaac gaggcctggt ggagaaactc gataaaaacc tatactgcca
    16801 gaactgtcct attttcggat agacaaacta gtagttttaa aaagatcatc cgatccaatc
    16861 atttcaaaaa atgcaaaact gatatcagga tcatttagct caggcttgtt atcgtaaagc
    16921 atttgtaaag gttgcattta gttaggagta aacgcgaaac tatcatcatt atttgctgaa
    16981 gaagatgaag aagctaattc agagtttgtt tgaaataaag gagacgtgct gacagtttct
    17041 ttaccctgtt ttttgcagag gtagaaactt ttatatcgga agtttctacc ggagaatttt
    17101 tctcatttgt agaaattgtt gaatcgatac tattcgaaat ttgcgtatct gaaggtatgt
    17161 tctttgatgc agaagtccca caagcagtaa gtaacaataa atttgataaa ctgataaaca
    17221 gaattctttt cattatgtac acactcctta tagtatcgtt tagcatacac ctatgctcag
    17281 tattaggcaa aatcaatgca aaactatcaa gtcttttata gtatctatta gtcttagtaa
    17341 tacatatatg gagagggtga tatcccagtt caaaccagat acattggtta ccatctgtat
    17401 taacgattat gcctctaaac gcttttttta tgaaaaaatt tataagtgaa aacgtaggta
    17461 tgatattatc tagaggtaat ttttttgggt atttaaatag ttgctaggag gatagctttt
    17521 ggatttttac agtaatttta tcttaataat agcaatatta ttacttctaa atatttggtt
    17581 ttttgataag agtagaaatg cagggatagg ttttagaacc aaacgaagta cgtcttctga
    17641 aaaaaagtgg gtatattcac agactatttt ttacggaggg gttatttcaa tcagtttact
    17701 ttcttcaaca ctttattctt ttaatgttat agacgtttcc atgtcaaatt ttatatctat
    17761 tattggaatc cttatttctg caattattac acaactactt cttgtgtttg aagagaagtc
    17821 aaaaaataat taaagcaatt atgcctttta gtacaggaat tattacttct tacaaatgta
    17881 tagcgaatgt gaagcctgct gttttaattg ttctagaaaa acttttgagt gaacaattat
    17941 tttatgttag gatgaattaa tttctctaga gtttata
//
LOCUS       DACDYM010000052        17628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDYM010000052 DACDYM010000000
VERSION     DACDYM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.052"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IWM59_001830"
     CDS             287..694
                     /locus_tag="IWM59_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979858.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVNLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IWM59_001831"
     CDS             708..1109
                     /locus_tag="IWM59_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979859.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IWM59_001832"
     CDS             1111..1482
                     /locus_tag="IWM59_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979860.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="IWM59_001833"
     CDS             complement(1518..1820)
                     /locus_tag="IWM59_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979861.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2069..2269)
                     /locus_tag="IWM59_001834"
     CDS             complement(2069..2269)
                     /locus_tag="IWM59_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9979862.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2574..3806)
                     /locus_tag="IWM59_001835"
     CDS             complement(2574..3806)
                     /locus_tag="IWM59_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9979863.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4063..4632
                     /locus_tag="IWM59_001836"
     CDS             4063..4632
                     /locus_tag="IWM59_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9979864.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4810..5250)
                     /locus_tag="IWM59_001837"
     CDS             complement(4810..5250)
                     /locus_tag="IWM59_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9979865.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5408..6172
                     /gene="map"
                     /locus_tag="IWM59_001838"
     CDS             5408..6172
                     /gene="map"
                     /locus_tag="IWM59_001838"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9979866.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6204..7127
                     /locus_tag="IWM59_001839"
     CDS             6204..7127
                     /locus_tag="IWM59_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9979867.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7203..8342
                     /locus_tag="IWM59_001840"
     CDS             7203..8342
                     /locus_tag="IWM59_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9979868.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8335..9135
                     /locus_tag="IWM59_001841"
     CDS             8335..9135
                     /locus_tag="IWM59_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9979869.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9135..9962
                     /locus_tag="IWM59_001842"
     CDS             9135..9962
                     /locus_tag="IWM59_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9979870.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9940..10674
                     /locus_tag="IWM59_001843"
     CDS             9940..10674
                     /locus_tag="IWM59_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9979871.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10774..11640
                     /gene="rfbA"
                     /locus_tag="IWM59_001844"
     CDS             10774..11640
                     /gene="rfbA"
                     /locus_tag="IWM59_001844"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9979872.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11654..12226
                     /gene="rfbC"
                     /locus_tag="IWM59_001845"
     CDS             11654..12226
                     /gene="rfbC"
                     /locus_tag="IWM59_001845"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9979873.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12248..13276
                     /gene="rfbB"
                     /locus_tag="IWM59_001846"
     CDS             12248..13276
                     /gene="rfbB"
                     /locus_tag="IWM59_001846"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9979874.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13374..14225
                     /gene="rfbD"
                     /locus_tag="IWM59_001847"
     CDS             13374..14225
                     /gene="rfbD"
                     /locus_tag="IWM59_001847"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9979875.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14259..16292
                     /locus_tag="IWM59_001848"
     CDS             14259..16292
                     /locus_tag="IWM59_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979876.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16336..17616
                     /locus_tag="IWM59_001849"
     CDS             16336..17616
                     /locus_tag="IWM59_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9979877.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         5535 a         2966 c         3586 g         5541 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taaacttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtgtaatagt tagtcaggaa cgtacaaata
     2041 acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta
     2101 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
     2161 tagatgcatg cgtatgtctt agtccctcaa taaacagtcg aagagaaaat aggcaaaaat
     2221 tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaacaga
     2281 ttcgaattaa acgatttcga ttggcttata ccagtcctat ccgacgaaag atatatcgtt
     2341 cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat
     2401 gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa
     2461 gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt
     2521 agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc
     2581 caatcgccat ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc
     2641 atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct
     2701 gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct
     2761 agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat
     2821 tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca
     2881 tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaatgact
     2941 tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc
     3001 tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc
     3061 gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa
     3121 agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata gtgcagggca
     3181 gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc
     3241 catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat
     3301 gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca
     3361 gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt
     3421 agtgctttgc cagccgctga ttgataagac gctactcgat ggctatctac accagcaaat
     3481 gcatcgggat cggcagaaac aacactgatc ctgcttgctc ctttttcaag ccattcatcc
     3541 gcttgatcga ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga
     3601 aattctcttt ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta
     3661 taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca
     3721 actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt
     3781 agaagttcat taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa
     3841 ctattagcat gataccattt aattatgatt caaacaatga ggcctttaaa taaaaaaaag
     3901 aggttatttt tttaaggtta agtttcatga tatatgttac tacttgctat ttcagcttgt
     3961 gaagaaaatc aaaaactaat tttgcatatc aaaaaaaaca tgttacaatg aaattaatca
     4021 atggtggaag cgttataaaa aaattaagga gattaaaaaa atatggcaag aaaaaagacg
     4081 attactagag aacagatttt ggcagctgct tatgaagtag ctgccaccga tggatttaca
     4141 cgatttactg cgagaaacat cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt
     4201 gagtttaaaa atatggatga cttgagacaa gctctttttg agcagatcta tgatcattta
     4261 caatatgaag tgttcactgt tgagcataca gggaatacga ttatcgatct tgcttttaat
     4321 tatatccatt ttgcaagaag agagaagaag ttgtatcgtt cactgtactt agaagaatat
     4381 ggtggaggaa aagagatgca gaatttctct tatacttact ttgtgaatac agtgaaacgc
     4441 gatccagctt atgtgaatct ttcagaagag cagatcgaat ctttgtataa tgggacatgg
     4501 atcatagcta ctgggatcgc agctttgatg tcttcaagca tcatccatcc aactgatgaa
     4561 cagattgaaa agatgataca agacagtata gatgctatct tgaaattcaa agaaccaata
     4621 gaaatcgatt gaatatactc aaaagttcca caaatcagta gataacaaga gatgtgtcaa
     4681 aataaggaaa agttcgtttt tcttacagcg tatgcatttt taactcccga agaactcttc
     4741 catgaatgca atgaataaaa gagccgtaca gattgaataa atctggatgg ctcttttatt
     4801 gcaagtatat taaatttttg cgactagttc tttagcgaaa gcttctaaat tttcgatatc
     4861 ttcttcttca gcattcagat ccactttaac attttcggct ccttttgatg cgcctgtttt
     4921 tgcaaaagca gcatcgaaat catccaccga tttgcagaat tcatcgtaaa aagtatcacc
     4981 agatccgcag acaccgtaaa tttttcccga tagatccaat tcttgcaagt cttcataaaa
     5041 gtctacgatc tcatcaggca gatcgccatc atcgtatgta taagtagcta ctacgcaaat
     5101 atctgcctcc tcgaattcat ctgcatctac ttgggtacat tcgttgattt cgacttcgat
     5161 atccagattt tcaaatgctt ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat
     5221 gctagcatat acgatttttg ctaaagccat gatgtgcctc ctcgtttata attatttaac
     5281 aattatgaat tataccaaaa catcattgaa aagaaaataa aacctaaaga cacctgggga
     5341 atgtcatttg gctaaatgta tgccgctatg ttaaaatgag agggatttgt aaaaaggaga
     5401 cgaaaatatg attactttaa aatcagcacg tgaaattgaa atgatggcag aatctggagc
     5461 tttattagca gatgtacata aaaatctgcg tgattttata aaaccaggaa tcacaagttg
     5521 ggatattgag gtatttgtca gaaactatat cgagagtcat ggcggaattg ctgcgcaaat
     5581 tggctttgaa gggtatgaat atgcgacttg tatcagtata aatgatgaaa tttgtcatgg
     5641 attccctcgt aaaaagcctt tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga
     5701 tttgaaaggc ggcatttctg attcttgttg ggcctatgta gttggagaat ctacacctga
     5761 gatcgaccga ttgatggaag taacgaagaa agctttgtac atcggtattg agcaagcaca
     5821 agtagggaat cggattggtg acatcggtca tgctatccaa acttatgtag aaggcgaaaa
     5881 tctagcgatc gttcgtgatt ttattggtca tggcgttggt cctacgattc atgaagaacc
     5941 agcagttcct cattacggag aagctggaaa agggttgcgg ttgaaagaag gcatggttat
     6001 cacgatcgaa cctatggtta ataccggaac ttggaaaatg aaaatggatc ctaacggctg
     6061 gactgcgtat acaagagacg gcggactttc ttgccaatat gaacatacat tggctattac
     6121 taaagatggt ccaagaattt tgacatcaca aggagaagaa ggcacttatt gatctatcca
     6181 gtcaataggt acgggagagg tacatgaaag tttggaacaa aataaaacaa aatgaaaatc
     6241 tgatgcgctt tattgagacg actcaaaagc gcatcactga ttcagaaatg agtacgacct
     6301 ctgtcgtcgt tgcttattat ttactcttgt ctttatttcc attgattatt gcttttggta
     6361 atattttacc gtttctccac atcgatcaag agactgtact gacctattta cgagaagtga
     6421 ttcctgagac cgtttaccag tttatcggtc ctgcaatcaa agaccttttg acacaaagtt
     6481 caggaggctt gttatccgtt tctgcattag cggctttatg gtctgcaagt caaagtatca
     6541 atgctttgca aattgcgatg aataaagcgt atggcgtaga aaatagaaaa aattttttga
     6601 tcgtccgttt cttctcacta attgtcattg tcttgttcat gattgctatt agtggggtaa
     6661 ctatcgttct tgggttggga cagctgattt tggatgctat tcagccaatc gtacaaatac
     6721 ccgtgaactt tatcgatcag ttccaaactt tgaagtggcc aatcacattg gtagctttat
     6781 ttgtcatcat gttcttgatt tatttaattg tccctaatgc tcagttgaag ttgaagacga
     6841 tcattccggg cacgatcttc gctactgtcg gctggatgct gttatcacaa gtgtttagta
     6901 tctatgctag gtattttgca acgaggatca gcggttatca gatcatagga agttttattg
     6961 tactcatgct ttggcttaat tttgctgcta cgatcatcat tttgggcgga atcatcaatg
     7021 cagttgttca agaatacgtg acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa
     7081 aatggttcaa taaattgaaa actaaatttt ctcataaaaa aaagtagaaa caagagttct
     7141 tattgtaaat ttatatcagt aatttataat aattatgact cttaattaaa gtaggtgccc
     7201 atatgcttgt ttcttttagt atggttataa gattttttct gacaatttta ttatcactga
     7261 tattaacacc tgtattcaaa atcatttcag tacaaacagg tatggtcgac aaaccaaatg
     7321 aaagaagaat aaataaagtt ccaatgcctt cagcgggcgg actgcctatc tttatttcat
     7381 ttgttatatc gactttattc ttattccgta aaattatccc tcaatcgtat attgtaccga
     7441 tcttgattgc ttctttagta atcatagtca caggagtgct cgatgataaa tatgagctga
     7501 cacctaaaca aaaaagtata gggatcttga ttgcttctct tattatttat tttgtagcag
     7561 atatacgtat tgattccttc acgcttcctt ttattggata tatccaacta ggctggctta
     7621 gttttcccgt aacgattttt tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg
     7681 gtttggatgg gctggcggca gggatttcat tgatcggttt aattacgata ggaatcattg
     7741 gatatttttt cctccatgct tctacagtgt atattccagt tgttattttc tgtcttgtag
     7801 caagtattat cggttttttt ccttacaatt tttatccggc aaagatttat ttaggcgata
     7861 caggagctct attcctaggg tttatgatgg ccgtcctttc actacaaggg ctgaagaacg
     7921 tgacttttgt ttcatcgatt tctttgctga tcgtcatggg agtgccggta acagacacgt
     7981 tttttgctat cattaggaga aaagcaaacc gtgtttcttt ttctacagca gataaaaaac
     8041 atcttcatca ccgactaatc agtttaggct ttactcataa aggtgctgtc ctgacgattt
     8101 atgcgatggc tttgatgttt tctttcacag ctatggtcat gaattatact ggaagaatcg
     8161 gaacgatcat attgattatt gccatgttgt ttgctgcaat tttattattt gaattgattg
     8221 gtctgatcaa tgagaaacat cagtttattt taaaaacatt gcgattttta gggaataaag
     8281 agtaccgtac gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat atagatgaat
     8341 aacaaattaa accagccgat ccttattagt attgtgacgg caaacagtga taaaattttt
     8401 cggacactag ataattttat ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt
     8461 tttgacaaca attcacttcc cgagtacaaa aaaagactaa cagagtatag gaaatatccc
     8521 tacatcgata tctattttca tgatgaaaat tccggatttg gttatggaca taatcacaat
     8581 ctgatagcta gcgattctcg ctatggcatt atttgtaacc cagatatttt agtggatgaa
     8641 gagacagtta cagcattggt cttgttattg aaagaacatc cggaatgtgc gatgttagca
     8701 cctaagattc ttaatgaaga tggaacaact cagcacctca tccgtgagca gctggctgtt
     8761 tttgattacg ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga
     8821 ctggctgtgt ttgaatgccg aggattgcct actgatgcta atagttatgt acggatgatc
     8881 tcaggtagtt ttatggtagc tgatattgat aaatttaaag agataaaggg gtttgatgag
     8941 cgtttcttca tgtactttga agacaacgac ttgtgtttga gttttgaaaa agcaggttac
     9001 aagttattgt acacaccctt ttatgcagtt atccattttt acgggaaggg tgcgcatcga
     9061 aatgcaaaac tgtttcgcgt gtttttgact tctatgatga aatttttcaa taagtggggg
     9121 tggcgttttt tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat cagcaactat
     9181 tcagtcaatc tccagcttat ggaccattaa gcaaagcact tagcgaaaag caaattcagc
     9241 ttattattta cgataatagt cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata
     9301 tcgtttatca tcatgatccg caaaatcctg gtttagcggt ggcttataat tttgcgttgt
     9361 ctcaagcaga tgaaggaaca cgttgtctcg tcactctgga tcaggatacg aggctagtcg
     9421 acgattattt tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc
     9481 cgatgatttt ttctgggagc cgacaagttt ctcccctata ttcagatcgt tatatcaatc
     9541 gtacagctca agcagtagaa gttgaaggcc caacagctga acgaatcatg gcaatcaatt
     9601 caggtgttgc ctggtctgtc aaatttctaa aaaagattgg cgggtttaat cctgcttttt
     9661 ctttagattt tttggatcat tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg
     9721 agatccttcc tgtacgaatg gaacatgatc tttcggtttt agactatgaa aaagtttcaa
     9781 gtaatcgata ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa
     9841 ggcatttatc cagtcaccga cgacaattat tgttaagaac agctaaacag ttcttgaccg
     9901 tcaaaaaccg gcatatctgg cggatgactt tgcgctcata tgttaagaat tggaaggtgt
     9961 gaattcgtgc gttcggtttg tatagctacc tttaatggag gcagatattt aagagagcag
    10021 ttagatagta ttttatctca gctttcagca gaagatgaag tagtcatttc tgatgatggg
    10081 tcagtagatg atacatggga tatattaact gaatacgcag aaaaagataa gcgaatccag
    10141 cttttttcgg ggcctcgtca aggattgatc gctaattttg gttatgcgat ccagcagacg
    10201 aaaggagatc taatcttttt ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa
    10261 aagatcactg actattttga gcagaatccg gatcagctag tagtagtttc tgatttaatc
    10321 attgtggaca gcgagctgca agagatccat gattcgtatt tttcgtttcg aaattccaaa
    10381 gaaggatggc ttaataatgt gataagaagt cactatattg gtgctggtat ggctttccgg
    10441 tcttctttaa aacccgatat tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg
    10501 attggtttgc tgggaggaaa gaagactggg ttcctaagag aaccgttaac tctgtaccga
    10561 agacatgatt taaacgcgag tgagattgaa acacattcta aattacggca aaaaatttct
    10621 tggcgtgttc atctgattac ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag
    10681 atgtttaaat ccttaatatt tccatgacaa atataacaat tgatgagata ataacaaagt
    10741 gaaatttgaa atcaaaaagg agagcagata tccatgaagg gaatcattct tgctgggggc
    10801 agcggaacac gtctgtaccc tttaacaaag gcaacatcga aacaattgat gccgatttat
    10861 gacaaaccaa tgatttatta tccaatgtct actctgatgt tggcgggaat caaagaaatt
    10921 ctgattattt caacgccaca agatacacca cgtttcaagg aattatttgg aaacggaaat
    10981 gatttaggac tgcagatcga atatgcagta caagaaagtc cagatggatt ggcacaagcg
    11041 tttatcattg gagaagaatt tattggagat gatagtgtct gcttggtatt aggggacaat
    11101 atttactatg gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc
    11161 gcgacagtat ttggctatca tgtcaatgat ccagaacgct ttggtgtagt agaatttgat
    11221 gagaatatgc aagctttgtc gattgaagaa aagcctgctc agccaaaatc aaattacgct
    11281 gtaactggcc tttattttta tgacaatgag gtaatttcta tcgctaaagg tatcaaacct
    11341 tcagaacgtg gagaactaga gattacggat gtcaataaag catatttgga taaaggaaaa
    11401 ctatccgtag aacttatggg acgcggattt gcttggcttg atacaggaac acatgaatca
    11461 ttgcttgaag catctacctt tatcgaaaca attgaaaaac gtcagaactt aaaagtggct
    11521 tgtttggaag aaattgctta ccgtatgggc tacatcgata aggaacaatt gctggccctt
    11581 gcacagccat tgaaaaaaaa tcaatacggt caatatttat tgaatttggc cgctgaataa
    11641 ggagtcctta cagatgaaag taacagatac aaaattaaca gatgtcaaaa tcgtagaaat
    11701 ggacgtcttt ggcgatcatc gcggtttctt cacagaaagt tattcaaaag caaaatttgc
    11761 agaacatgga ttgaaatatg actttgttca agacaaccat tcactatcag ctgaagctgg
    11821 tgtccttcgg gggcttcatt tccaaaaagg agaagctgca cagacaaaat tgattcgggt
    11881 agtcactgga gcagttttag atgtagtagt ggatatccgc aaaggtagcc cgacttatgg
    11941 tcaatgggac ggatttatct tatctgaaca taatcatcga cagttgcttg tacctaaagg
    12001 atatgcccat ggattcgtta ctttgacacc aaatgtgaac ttcctttata agtgtgataa
    12061 ttactataat gcagaagcag atgggggcat cgcatttgat gatccagatt tagcgattga
    12121 ttggccaatt gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc atccgacatt
    12181 aaaagaattt gaagcagaaa atccatttgt ttacggagaa atctaagaat taaggagaaa
    12241 ttttcttatg aaaaacatca ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt
    12301 tcattacgtg gtaaacaatc atccagaagt gcatgtcact gttttggata aactgactta
    12361 tgcaggaaac aaagagaact tggctggact tcctagcgat cgcgtagaat tggtcgtagg
    12421 agatgttgca gatgcagaat tagtggaccg tctagtaaaa gaagcggacg cagttgttca
    12481 ttacgcggca gaatcccata acgataattc attaaaagat cctttcccat ttgtacaaac
    12541 gaatatcatt ggtacgtata cacttttaga agcttgccgc aagtatgatg tacgctatca
    12601 tcacgtatct actgacgagg tttacggcga tcttccatta cgtgaagacc tgccaggaca
    12661 tggggaaggc gaaggagaaa aattcactgc ggaaacacca tataatcctt caagtccata
    12721 ttcttctaca aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt cttttggctt
    12781 acgtgcaacc atttcaaact gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt
    12841 tattcctcgc caagtaacga atatcttaag cggtattcgt ccaaaattat atggcgaagg
    12901 aaagaatgtt cgtgactgga tccatacgaa tgatcattct tcagcagtgt ggctgatttt
    12961 gacgaaaggc agaattggcg aaacctatct gatcggtgca gacggtgaag aagataataa
    13021 gaccgtgatg gaattgatct tagaaatgat gggtcaacca aaagatgcgt acgaccatgt
    13081 aaacgaccgt gcaggacacg acttgcgtta tgcaatcgat tcgacaaaat tacgggaaga
    13141 gttaggatgg aagcctgagt ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg
    13201 gtacgaagaa catgaagact ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc
    13261 caaaaatgga caataataca gagaaaatgt cactgtatta atggaaaaac acatgtgagg
    13321 caggcgctcg cctgtctctt ttttaaaata aaaatcaagg agcggataat catatgtttt
    13381 tattaactgg aggaaatggg caactaggta cagaattgcg ccatttatta gatgaaaaaa
    13441 atctgcaata tgtttcaaca gatgcaaaag acttggatat cacagatgcc gagaaaacaa
    13501 tggcttacat cacagaatta aaacctgatg ttattttcca ttgtgcagct tacacagcag
    13561 tagataaagc agaagaagaa gcaaaagaat tagatgagaa gatcaatgtt gatggtacaa
    13621 gaaatgtagc acttgcagca aaagctgtga atgctcaatt cgtctatatc agtacagact
    13681 atgttttcga tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa acgaatccac
    13741 taaatgaata tggtcgtacg aaattattag gcgaaaaagc agtagaagag attttagaag
    13801 actattatat catacgaact tcttgggttt tcgggatcta tggacacaat ttcgtcttca
    13861 cgatgcaacg cctagcggaa acccgcgaca aactgactgt agtaaatgac cagtttgggc
    13921 gaccaacttg gacacgtact ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac
    13981 catttggtat ttatcatttg tcgaacgaaa atagctgttc ttggtatgaa ttcgcaaaag
    14041 aaatattgaa agatacagat gtagaagttg cacctgttac gtcagaagaa tttccgcaaa
    14101 aagcaacacg tccgcagtat tcagtcatga gcctgaaaaa gacagaagag ttaggatttg
    14161 tcataccaac ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa aaaatgaata
    14221 agtaatttcc ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa acaagaagtg
    14281 cacgacgaaa agaaaagcca aaagcgaacc aggcagattc gaatttggtg cttgacctgt
    14341 ttctaaaact ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt acttttaatt
    14401 taaacggaag ttcacttggt gtttgggata aatatgtgtc gcaaagagac gatgggaaaa
    14461 aaactgatgt gatttttgga cagaatcggg aagtgcggtc agacgaatgg ctggtgcaga
    14521 caccttttta cctctcacaa gctgaagagg actttccgtt ggtgaacgag gattattcat
    14581 taaacggaca aaacatgatc attgcgtata attctcctgc aaaggatatt acggttatcg
    14641 gaaaaccatt caactgggga ttctttttcc tcggaagaga tcgaggactt tccttttatt
    14701 gggcgatgaa attagtcgtc atggtcttgc ttggattcga agtactgatg atattaacga
    14761 agagaaaaaa agctctatcg ctaatcggtg catttgcgct gacattttct ccagcagtcc
    14821 agtggtggtt catgcaacat gtgggcgatt tgattttctt tactttggga ctgatgactg
    14881 ctttttatca ttatttctac aatcatgata aaaaatggct acgaacgttg atgatgcttt
    14941 tggtggttat tttcggtatt gggtttattt tagtgattta cccagcccat caagtgatgc
    15001 tggcgtactt gctgattttt tattttgttg gcctgcttat ttactatagc aagaaagttt
    15061 catgggatct gttggatgta gtgttgattg gaggggcagt tttatttatt ggcggagtca
    15121 tggttcattt ctggctaacg tcaaaagacg cattgttagc ttctttgaat accttatatc
    15181 caggaaaacg ggtaagtact ggaggaaagt ggacgattgg cgaatttttt tctttcctta
    15241 caaattggaa aattcctttt gaagatatca actattcgaa taactctgaa gtggccctgt
    15301 tctatcactt ttttccaact gtattcctag caagtccatt tgtgttattt ggcaaaaaag
    15361 atagcgaaca aaagttactg ggacgtattt tgatgatttt ttgtctgttt tctatctttt
    15421 ggattaccgt tgggttgcca aaatggctag cagaaatcac tctgctgtct tatgttcctc
    15481 cattaagagc catccttact tttagttttg cggcttgttt attatcgatc tggtttatcg
    15541 cttatatttg gagagaagat gttattccta tttggtataa attcattttg ttggcagtaa
    15601 atgcatggtc ttatttctat gcgattacgc attcgaaaat agataattat ttttcggatt
    15661 ttgaaaccat cgcagttgct gtgttgggaa cagtgatttt agcatttgca ttatttggct
    15721 ggagacgctt gttttacctc gcttttgccg ccttagttat cgtatctggc tttttcgtca
    15781 atccagtcgt agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa gaaatccaaa
    15841 aaatcgatca acaagatcct ggtaaagttt ggttgtctga agatgaatta tataacttca
    15901 ccccaacatt gggagtgaaa tccttcaata cagtacgctt ttatccagat atagctgcat
    15961 ggaaggaaat cgatccgaaa gaagaatacg agaagtatta taatcggtat gctcatacaa
    16021 gagcttttat cgctaaggaa gatacaaagt tcattttaga tcgtccagat atgttttcgg
    16081 tcacgttgaa ttttgaagac gcagaaaaat tagggatcga ctatgtgatc agtaaacgac
    16141 cattaactga ttacaaccaa ttatatgaag cgcagttcac gcagctatat ggaccggata
    16201 aagacggtta tatgattttt aaagtagaat atccggatta tccagatgta ccacaaacac
    16261 aaactcagga aaatcagcta gtcgtacagt aataggctag ctgagaataa tcctgaaata
    16321 ttagtagaaa ggagagtgga gataattcaa gcaatcgtac aaaaatttgt caacaaaatg
    16381 agtgtcggtt tcttgctttt gttgctgacg ctagtctttt ttgtttggac agcagggtta
    16441 ggttttccag caactttgtt tatatataac aatagttcat tacttatcgc ttttagtgtg
    16501 gctattttgc tcattttaaa tgtttataaa gcaaaaggcg tcgattttct ttatctaggt
    16561 gttgcgcttg cccttttcat tttcttttct tactttcggg aattaagaaa tgagtcttta
    16621 tttggagatt tactgtttcc tttgattatt ttattttgtt tagtaattaa atggatcgag
    16681 tggacaaaat gggatcgcgg tatttatctg attgtcttta cggtcttcct cggaatcacg
    16741 ttatatcgtg tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc
    16801 aattcgcttt ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt
    16861 gctttattga tcagtggatt tgccagttct tttgaacgat ggtatgtacg aatcatcggt
    16921 gcagtagcag ttgttgcagc ttttctcggg atttgggtct gccaatctag aggtgcatta
    16981 gtcgcgttga ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt
    17041 cgtgcgccat ttattgctta cacggttacg attcttttag ctttgccgat ttcttatctg
    17101 gcggctgtct ctgaaaaagt caatttgttt accggtcgag aagatatttg gcataaattc
    17161 tatcagacgc taggagaaaa atctgaacaa gttttactag gaatgaaaac atttatcttc
    17221 caacgaggaa accaattttt aggaaaccac aatagttata attctatttt gaatatctat
    17281 ggattgatag gatttgggat tgctactttg ctgcttatcc tttttattgg tcgcttaacg
    17341 ttgaaagcag atttatccaa cgggcagatg acttttatct gggccttttt tgcggtgatg
    17401 atgcagtcat ttatggagga tacattgact tcatacccat gggttccgat tatttatatc
    17461 ctgttggcta tggccgttca tcgatatgat gaaccagaag ttgcggagga attctatgtt
    17521 atggaagagg catatgacga tatgacttat gatgatacat cttatgagga acaaccagat
    17581 agcgctcatt cttccaggat ccaacgctat cattgatagg cacaaaat
//
LOCUS       DACDYM010000053        17299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDYM010000053 DACDYM010000000
VERSION     DACDYM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.053"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            232..1500
                     /locus_tag="IWM59_001850"
     CDS             232..1500
                     /locus_tag="IWM59_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAP9979878.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="IWM59_001851"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="IWM59_001851"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9979879.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3076..3351
                     /locus_tag="IWM59_001852"
     CDS             3076..3351
                     /locus_tag="IWM59_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9979880.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3357..4124
                     /locus_tag="IWM59_001853"
     CDS             3357..4124
                     /locus_tag="IWM59_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9979881.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4292..6121
                     /gene="typA"
                     /locus_tag="IWM59_001854"
     CDS             4292..6121
                     /gene="typA"
                     /locus_tag="IWM59_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9979882.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6382..8154
                     /gene="recQ"
                     /locus_tag="IWM59_001855"
     CDS             6382..8154
                     /gene="recQ"
                     /locus_tag="IWM59_001855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9979883.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8268..8570
                     /locus_tag="IWM59_001856"
     CDS             8268..8570
                     /locus_tag="IWM59_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9979884.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8573..9736
                     /locus_tag="IWM59_001857"
     CDS             8573..9736
                     /locus_tag="IWM59_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9979885.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9762..13190
                     /locus_tag="IWM59_001858"
     CDS             9762..13190
                     /locus_tag="IWM59_001858"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9979886.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13211..14335
                     /locus_tag="IWM59_001859"
     CDS             13211..14335
                     /locus_tag="IWM59_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979887.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14325..14762
                     /locus_tag="IWM59_001860"
     CDS             14325..14762
                     /locus_tag="IWM59_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9979888.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14746..15084
                     /locus_tag="IWM59_001861"
     CDS             14746..15084
                     /locus_tag="IWM59_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9979889.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15203..15763
                     /gene="rsmD"
                     /locus_tag="IWM59_001862"
     CDS             15203..15763
                     /gene="rsmD"
                     /locus_tag="IWM59_001862"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9979890.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15760..16251
                     /gene="coaD"
                     /locus_tag="IWM59_001863"
     CDS             15760..16251
                     /gene="coaD"
                     /locus_tag="IWM59_001863"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9979891.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16241..17284
                     /locus_tag="IWM59_001864"
     CDS             16241..17284
                     /locus_tag="IWM59_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9979892.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5866 a         2998 c         3826 g         4609 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     3001 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     3061 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     3121 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     3181 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     3241 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     3301 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     3361 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     3421 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     3481 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     3541 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     3601 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     3661 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     3721 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     3781 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     3841 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     3901 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     3961 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     4021 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     4081 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     4141 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     4201 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     4261 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     4321 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     4381 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     4441 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     4501 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     4561 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     4621 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     4681 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     4741 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     4801 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
     4861 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
     4921 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
     4981 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
     5041 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
     5101 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
     5161 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
     5221 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
     5281 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
     5341 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
     5401 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
     5461 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
     5521 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
     5581 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
     5641 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
     5701 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
     5761 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
     5821 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
     5881 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
     5941 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
     6001 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
     6061 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
     6121 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
     6181 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
     6241 gccgaaagac gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
     6301 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
     6361 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag
     6421 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
     6481 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
     6541 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
     6601 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
     6661 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
     6721 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
     6781 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
     6841 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
     6901 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
     6961 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
     7021 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
     7081 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
     7141 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
     7201 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat
     7261 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
     7321 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
     7381 atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa
     7441 gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa
     7501 tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta
     7561 gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa
     7621 cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa
     7681 caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa
     7741 tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc
     7801 atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga
     7861 caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc
     7921 gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt
     7981 cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca
     8041 agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc
     8101 gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa
     8161 cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa
     8221 aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg gattctttgt
     8281 cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc
     8341 gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata
     8401 cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa
     8461 agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg
     8521 aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa
     8581 gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg
     8641 tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca
     8701 aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat
     8761 cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc
     8821 catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg
     8881 ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat
     8941 catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga
     9001 ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc
     9061 tattcttccc gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt
     9121 agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag
     9181 tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca
     9241 gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg
     9301 gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt
     9361 aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc
     9421 aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt
     9481 tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt
     9541 gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac
     9601 tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt
     9661 gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa
     9721 gagcttaggt ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg
     9781 ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa
     9841 ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag
     9901 catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca
     9961 tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg
    10021 aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac
    10081 tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg
    10141 ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt
    10201 ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg
    10261 gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg
    10321 aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta
    10381 agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa
    10441 gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta
    10501 tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg
    10561 ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg
    10621 aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata
    10681 tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc
    10741 ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag
    10801 acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg
    10861 gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt
    10921 ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga
    10981 aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta
    11041 tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg
    11101 atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga
    11161 aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat
    11221 tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta
    11281 agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa
    11341 atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg
    11401 ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct
    11461 tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag
    11521 atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc
    11581 ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat
    11641 ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact
    11701 ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg
    11761 cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt
    11821 attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag
    11881 atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa
    11941 cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt
    12001 attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg
    12061 ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc
    12121 tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca
    12181 tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg
    12241 aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc
    12301 atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg
    12361 ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg
    12421 atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg
    12481 tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa
    12541 agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag
    12601 tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag
    12661 acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata
    12721 atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg
    12781 gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa
    12841 tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac
    12901 gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg
    12961 aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag
    13021 tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa
    13081 tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag
    13141 gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag
    13201 gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc
    13261 attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca
    13321 acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc
    13381 tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg
    13441 attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac
    13501 atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc
    13561 tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac
    13621 tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga
    13681 agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt
    13741 ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt
    13801 gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc
    13861 ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat
    13921 gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag
    13981 tcagctgcta ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat
    14041 ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa
    14101 tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat
    14161 tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca
    14221 tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat
    14281 gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag
    14341 agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa
    14401 cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt
    14461 tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt
    14521 atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata
    14581 tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc
    14641 aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc
    14701 cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat
    14761 agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa
    14821 ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc
    14881 gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat
    14941 tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa
    15001 ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa
    15061 atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac
    15121 ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt
    15181 accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt
    15241 aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt
    15301 caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg
    15361 tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa
    15421 ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt
    15481 taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca
    15541 attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca
    15601 taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa
    15661 aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg
    15721 cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca
    15781 ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta
    15841 ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca
    15901 gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg
    15961 catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc
    16021 agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat
    16081 catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg
    16141 agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg
    16201 ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt
    16261 gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt
    16321 gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa
    16381 tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc
    16441 tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga
    16501 agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga
    16561 ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat
    16621 ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct
    16681 ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt
    16741 tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa
    16801 tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg
    16861 aataggtatc acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa
    16921 ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca
    16981 agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag
    17041 tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc
    17101 tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta agaaagaata
    17161 tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc
    17221 aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa
    17281 atgaaactaa ataattaaa
//
LOCUS       DACDYM010000054        17007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDYM010000054 DACDYM010000000
VERSION     DACDYM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.054"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..126)
                     /locus_tag="IWM59_001865"
     CDS             complement(<1..126)
                     /locus_tag="IWM59_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP9979893.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="IWM59_001866"
     CDS             complement(230..541)
                     /locus_tag="IWM59_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9979894.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="IWM59_001867"
     CDS             complement(543..740)
                     /locus_tag="IWM59_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9979895.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="IWM59_001868"
     CDS             complement(1134..2861)
                     /locus_tag="IWM59_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9979896.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="IWM59_001869"
     CDS             complement(2854..4047)
                     /locus_tag="IWM59_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9979897.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="IWM59_001870"
     CDS             complement(4234..4878)
                     /locus_tag="IWM59_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9979898.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="IWM59_001871"
     CDS             complement(4972..5106)
                     /locus_tag="IWM59_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9979899.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="IWM59_001872"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="IWM59_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9979900.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="IWM59_001873"
     CDS             complement(7237..7710)
                     /locus_tag="IWM59_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9979901.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="IWM59_001874"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="IWM59_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9979902.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="IWM59_001875"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="IWM59_001875"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9979903.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="IWM59_001876"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="IWM59_001876"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9979904.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="IWM59_001877"
     CDS             complement(11757..12896)
                     /locus_tag="IWM59_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9979905.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="IWM59_001878"
     CDS             complement(13205..13918)
                     /locus_tag="IWM59_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9979906.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="IWM59_001879"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="IWM59_001879"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9979907.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="IWM59_001880"
     CDS             15107..16639
                     /locus_tag="IWM59_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9979908.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="IWM59_001881"
     CDS             complement(16830..>17007)
                     /locus_tag="IWM59_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9979909.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DACDYM010000055        16639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDYM010000055 DACDYM010000000
VERSION     DACDYM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.055"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(2..1507)
                     /locus_tag="IWM59_001882"
     CDS             complement(2..1507)
                     /locus_tag="IWM59_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9979910.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1504..2190)
                     /locus_tag="IWM59_001883"
     CDS             complement(1504..2190)
                     /locus_tag="IWM59_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9979911.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2383..3804)
                     /gene="gndA"
                     /locus_tag="IWM59_001884"
     CDS             complement(2383..3804)
                     /gene="gndA"
                     /locus_tag="IWM59_001884"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9979912.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(3974..4153)
                     /gene="rpmF"
                     /locus_tag="IWM59_001885"
     CDS             complement(3974..4153)
                     /gene="rpmF"
                     /locus_tag="IWM59_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9979913.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4211..4780)
                     /locus_tag="IWM59_001886"
     CDS             complement(4211..4780)
                     /locus_tag="IWM59_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9979914.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            4966..5868
                     /locus_tag="IWM59_001887"
     CDS             4966..5868
                     /locus_tag="IWM59_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9979915.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(5903..7687)
                     /gene="pyk"
                     /locus_tag="IWM59_001888"
     CDS             complement(5903..7687)
                     /gene="pyk"
                     /locus_tag="IWM59_001888"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9979916.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7761..8723)
                     /gene="pfkA"
                     /locus_tag="IWM59_001889"
     CDS             complement(7761..8723)
                     /gene="pfkA"
                     /locus_tag="IWM59_001889"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9979917.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(8891..12205)
                     /gene="dnaE"
                     /locus_tag="IWM59_001890"
     CDS             complement(8891..12205)
                     /gene="dnaE"
                     /locus_tag="IWM59_001890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9979918.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12436..12621
                     /locus_tag="IWM59_001891"
     CDS             12436..12621
                     /locus_tag="IWM59_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9979919.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12683..13879)
                     /locus_tag="IWM59_001892"
     CDS             complement(12683..13879)
                     /locus_tag="IWM59_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9979920.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14049..15485)
                     /locus_tag="IWM59_001893"
     CDS             complement(14049..15485)
                     /locus_tag="IWM59_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9979921.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            15778..16587
                     /locus_tag="IWM59_001894"
     CDS             15778..16587
                     /locus_tag="IWM59_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9979922.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4871 a         3509 c         2927 g         5332 t
ORIGIN      
        1 tttacttgtc ttctgccttt gcgataggaa taaagattct aaaaattgtc ccttgcccta
       61 ctacactttc tgctaagata cgtcctttat agttttcaac cagttgttta gcgatagaca
      121 accccagacc atttccaccc ttcgttctag ctcttgcttt atccactcga taaaaacgat
      181 tgaagatctt attaaggtct tctttgggta ttccttcgcc aaagtcctga atcgcgatct
      241 caaattcatt cagagtggaa gaaatagaga tatggacttc ttttcgtgta gttgaatact
      301 ttactgcgtt atccaatatg ataatgatta attgttcaaa gtgattacga taaatttgca
      361 gggtcacttc ttttgtcaaa tcatcatcta aagtgatcac atagtctgga tataatatct
      421 tgaaattatt ataaacttga taggtgactt cttttccttt agttgtttca ttcgcataat
      481 gaacatctac ttgttctgct cttgataagt ctagcatctc ttgaactaag cttttcatac
      541 ggctgatttc ttgcatgctg gcgcttaatg attcttctaa tatctcagga tcatctttcc
      601 cccaacgatt tagcagacta agatgccctt caataattgc aacaggtgtc cttaactcat
      661 gagatacgtc ttccacgaat tgctcttgtt gttcaatata caaacgcata cggtcaagca
      721 tctcattgaa tatttcagct aaatctgcca attcatcatt tgtgtcgatt tcaggcatgt
      781 ggatatctga ttgcggatct ttacgtatag tatccattgt atctctgagt accttgagtg
      841 gttttaagaa ataagaagat aaaaagaaac ctaaaatact acttaagatc aaagaaacaa
      901 cttctaaaac aataagagtc aataatagct tatttcgaat atcatagaaa gaagataatt
      961 catagaagct ttgcgcatag ccgatctttt ctcttgtacc tttagaatat ataggctgga
     1021 tagataaaaa gccagttttt ccttctagtg taacgatcgt tggaacttta cgtgtagtct
     1081 gaatcaggtt tcgactgttt tcatgggttt taaaaatcaa gttctcgtcc aaatcataaa
     1141 cataaagatc cattgatggc tgccctagct cagctataaa ggaatcgatc ttcattaagt
     1201 ttccttcgac cagtgaagta tgatctacga cctcatctcc gtcatttgtc gctttcgtca
     1261 gattacgata cgtatttact aatgtcagtt cttcatccga gttagccaaa cgagaagcta
     1321 cttctgaaat cgtacgctct acattgtttc tttcttttgc cacaatcaga ttgatagatg
     1381 atttataggt aatgacagcg aaaattgtaa acacaacgaa tatgaagaaa gaactcgcaa
     1441 aggcccactt gattgtcaag gagggccctt tcagttctct tttttccgat tttttcggct
     1501 ttatcacgag cgcatcacat aacctgttcc gcgtacagtt tggatatagc tttcctcccc
     1561 gggaacgtca attttgttgc gaagataacg aatatatacg tccactacgt ttgtttcaac
     1621 ttctgtttcg tatccccaca ctttatttag taaaacgtca cgtgctaata ccacgttcac
     1681 gttttccatt aaagtaagca gcaactcgta ttctcttttt gttagttcaa ttacttcatt
     1741 tccgcgacgt acgacacggt tttctttttc gatagtcaaa tcgcgataag taatagtagt
     1801 ttgtttagcc acatttttat cgccttcaat atcgattcga cgcaacaatg cacgaagacg
     1861 tgctaataat tcttcgatag caaatggttt gacgatgtag tcatctgctc cgtggtcaag
     1921 tccagataca cgatcgatca cagaatctct tgcagtcatc ataatgattg gtgtattttt
     1981 gacttggcgt acacgtctgc agacttctaa accgttcaat tctgggagca tcagatccaa
     2041 caatatggca tcccactcat tatttaatgc cgcatctaaa ccagttcggc cgttgtagtg
     2101 gacttctgcg ttataccctt catgcttcaa ctctagctcg acaaatcgtg ccaagttctt
     2161 ttcatcttca atgattagaa tattgctcat ttattatttt atcctttcta ttctactggc
     2221 tttttaaaaa ctctatcatt ataccgataa tatgcttaaa aatctatagt cattttatca
     2281 tggatagcaa ttttaaaaaa gagctgaagt ctactcataa caatcgactt cagctctttc
     2341 gtcctctttt tattttctca tttcctaaca gccgaaagac tattattctt catggtacca
     2401 tgagtaatgg aagattcctt ctttgtcgac acgctcatag gtatgtgcgc caaagtaatc
     2461 gcgttgggct tggattatat ttgcaggcag acgttctgct ctgtatgaat cgaagtaagc
     2521 gatggctgat gagaaagtcg gtacaggtac gccagcttgg acagccaagg caacgacgtc
     2581 tctgactgat tgctgatatt ttttcgtgat ttcaacgaag taatcatcta gcaataaatt
     2641 ttcgatctca ggatttttgt cataggcatc cgtaattttt tgcaagaatc gagctcggat
     2701 gatacatcca gcgcgccaga tttttgcgat ttcaccaaat ggcaagtccc aatcaaattc
     2761 ttttgaagcg acacgtaatt gagcaaatcc ttgggcataa ctcatgattt tactgaaata
     2821 caacgcttct cggatttttt cgaccaattc ttttttgtct cccgtgaact caaaatcgtt
     2881 cgtacgagac aaaatcttgc ttgcttgtac gcgttcttct ttataagcag aaatatagcg
     2941 ggcaaataca gattcagtga tcaatggaag tggcacacct aagtcaagcg cgctttggct
     3001 tgtccattta cctgttcctt tattcccagc tgcatcaagg atcacatcta ctactggttt
     3061 acctgttcct tcatcgtctt tacgtgtcag aatgtcagca gtgatttcta tcaaatagct
     3121 gtctaattcg ccttgattcc attctttaaa gatatcagcc atttcttcta ctgaaaggtt
     3181 taaaatattc ttcattaagt cataagattc agcaatcagt tgcatatctc catactcaat
     3241 accgttgtgt accattttca cataatgtcc ggctccattt gggccaatat atgtgacaca
     3301 tggtgctccg tcttctgctt ttgcagaaat ttgctctaag attggtgcaa ctagcgcata
     3361 ggcttctttt tgtccaccag gcatgattga aggtcctttt aaagctcctt cttcaccacc
     3421 ggaaacgcct gtaccgataa agttgatgcc agagttggct aactcttcat tacgacgcat
     3481 cgtatcttta aagaatgtat ttcctccgtc aatcaaaata tcgcctttat caaggtgagg
     3541 aagtaactct tggattgttg catctgttgc cggtcctgct ttgaccatta gcaagatccg
     3601 acgtggtttc tcgatagatt ctacaaattc ttcgattgta tatgttgctt tgaaattttt
     3661 atctgggtgt tcttctacta cagcggttgt tttagcccct gtacgattga ataaagccac
     3721 tgaatagccg cggctttcta tgttcaaggc tagattttta cccataacgg ccataccgac
     3781 aacgccgatt tgttgttttg acatatgaac tcctcctaca tacgggattt ctccctaatc
     3841 tcttacatta tagcttaaca cggctacatt gcaaactaaa gaacaacata ttttttattt
     3901 accgactaca aaaaaataaa cctcaaggct ccatggttag ccttgaggtt ttacattttc
     3961 taattaaaat gtattaagct tcttttgaca ttacgtcctt accatcatag taaccacaat
     4021 ttgcacatac gtgatggctt tttttcattt caccacagtt tggacattcg tttaaccctt
     4081 tgattgtcaa tttgtagtgt gtacgacgtt ttgctttttt agcttttgat gttcttctag
     4141 ctggtactgc cattttgcta cacctccttg taaagtcttg tatctaaatt agacacaata
     4201 tattccaatc ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt tcagatagtt
     4261 ttgcaagacg aggatcaatt ttattctcgg cttcttccat ttttctgcgc tggtattctt
     4321 cttcggaaac aacttcccaa tcattgcctt ttggaagttc tgttgctgct tcctcttctt
     4381 ttgtcaatac ccgcattgga atcgccaata agatgttgtc ttcaatcgac tctgctaaat
     4441 tcaataatgg accttcaatt agcaaaatct catctgcaga catttgttca tctctacttt
     4501 ggaattgttc ttctgtcatg aatacttcgt ctacagaaaa atccatgggc agttcaacag
     4561 gttctagtga acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa tgaacgatat
     4621 agcctttctt atcaactgag accaaacctt cgacttccac tggcgcaaca tcaagtatta
     4681 aattgtctcg cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa gttaacggtg
     4741 tctcctggta ttttctcaat tccaataaag accatttcat ttttcatcac tcctaagcaa
     4801 caaaagcaat tatacagggc aaaaagcatg ttgtcaatga aattttcttg acaccttgca
     4861 gttcataaaa aaacagaaaa atctttatta gaaagcctct gtttgctatc atcctcagaa
     4921 tcatttacaa taatcattat tccattaaag gagcggatga tcttcatgaa cacgaaagat
     4981 atcgaatact atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc taattatttc
     5041 cgtgtgagcc aacctactat tacatatgca ttacaacgtc ttgaaaaaga gtttgacacc
     5101 gtacttatta caagagatcg atcgcatcac gaattgatcg tcacgccttc tggaaagcag
     5161 ctgtttctcc atgcacaaac cattctccaa gagatttcta ctgcaagagc tgaaatcact
     5221 cagttgcaaa agaaaaagct tcgttttggc atgcctccta ttatcggcaa tgaatacttt
     5281 cctaaactgt caagttactt tatcaagcat gaattgatga atcaaattga gattaccgat
     5341 ggaggctctc gggacttata cgggatgatc cgacaaggga ggatcgattt agctgtatta
     5401 ggctcaacac agccaatcgt ggatcaatat ttagaaacag agttactttt ggacaaacgt
     5461 ttcatgatcg tcgtgccacc tacacatcct ctttccaagc aggatactgt ctctttcaaa
     5521 gatatagcag atgaacagtt tgtactattg aacgaacatt atgtccaccc cgcagcattc
     5581 actaagcttt ctaaacaaac acatactcat ccaaaagttg tttatcaaag caatgacttg
     5641 acgattttaa agagtatggt ccgagaaaat gtgggtatcg gttttttggc agagattgct
     5701 gtccatcctg aagatcattt agtttgcctc cctctttcag atgaagttca gcctcgcttc
     5761 ttgatttcat tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga acaagtagtc
     5821 caagttatcc atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa gaagagctgt
     5881 ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa acgatgccgc
     5941 gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg cctttagcac
     6001 caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa gtcaatccgc
     6061 cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct ttgtcagttg
     6121 atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca gcagatgaag
     6181 caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct aataatttag
     6241 aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact ggtacaccag
     6301 cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct tttttagctg
     6361 ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg taaacgcccc
     6421 agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag atatctgcat
     6481 ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca gcaacgattg
     6541 ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt tcagttacat
     6601 ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca ttttcagctt
     6661 cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg tttgcagatt
     6721 caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg tcagaagctt
     6781 ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta gcagtgataa
     6841 ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt ggtactagtt
     6901 cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca gaaactttga
     6961 tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag atttgaacat
     7021 gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt gctttacgta
     7081 caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata tcgtcagcat
     7141 cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc cctttacgtg
     7201 atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct tcgtcttttt
     7261 caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca acgtgaacat
     7321 cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca tgttcaggat
     7381 ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca cgaccaaaat
     7441 cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta tctaaaagaa
     7501 taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata cgtgataatt
     7561 gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca gattcaatca
     7621 attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg attttggttt
     7681 ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac acagcttaaa
     7741 tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata tagtgataag
     7801 tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac gatttcgttg
     7861 tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac agcgtaagca
     7921 ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg gacaacgtgt
     7981 cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc agcgaattca
     8041 tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc gcgatcgcgg
     8101 ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg gatgatgatt
     8161 tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt gcgtcccatt
     8221 acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg gtcaattgat
     8281 tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc aggaatatca
     8341 ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa agccattgcg
     8401 ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa ttttttcaat
     8461 tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg tgcagaatac
     8521 aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag acgacgaata
     8581 tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag tccttcgtaa
     8641 atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc tccgccactg
     8701 gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact ttatcctaag
     8761 tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta acattatttt
     8821 tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa gtagtatttt
     8881 ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata agttgccgct
     8941 ttatactctt gctgtcatcc acccaataag catctgacag aactactttt cgttgcgttt
     9001 tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc acttgtaatt
     9061 gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa gtttgtgaag
     9121 gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt tgcagttcct
     9181 gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca acggcaaaca
     9241 aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct ccactactgt
     9301 cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct ctaacttcct
     9361 tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc agctgaactt
     9421 gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa taaataccta
     9481 agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact tcttcttctt
     9541 ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata ttttggattt
     9601 ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca aaaactccta
     9661 ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc cgtatgagga
     9721 attgatccac tgactggaac gggccatttt gttttctttc gtttaatata tcttctacaa
     9781 aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc ttattgacca
     9841 actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt ttatttttac
     9901 gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa ttcatcaggc
     9961 tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat cccacgaatg
    10021 aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg atatattcat
    10081 atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat acaaaatgcg
    10141 acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc agaatatctg
    10201 actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt tcctgataga
    10261 cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg tagcgaatgg
    10321 cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg cctggtcgat
    10381 acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt cgtaaaacgt
    10441 tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt tgaaacaaga
    10501 gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac ccttctactc
    10561 gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct aaaaagtcca
    10621 ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga aaagcttcat
    10681 cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata actacacctg
    10741 ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt ttgaataaaa
    10801 gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt ttataagctt
    10861 gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt gcttcacttt
    10921 gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc attgtgccaa
    10981 atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa ttcaatactt
    11041 cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag cgttcggggt
    11101 ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg atttctaaca
    11161 cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg attttgtttc
    11221 tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat cccatttgat
    11281 ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag tcagtgattc
    11341 ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt tttccattag
    11401 gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa atcgattcag
    11461 ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat cgtttgatca
    11521 tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca gataaatttt
    11581 ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca aaaagttctt
    11641 cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct tttatcacat
    11701 actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata taaaaatttc
    11761 tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc tctttattga
    11821 aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg gtttctgtca
    11881 tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc atcagctgct
    11941 gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct tcataagtat
    12001 aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata cgatagaatt
    12061 caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag ccttgttttt
    12121 ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa taagaagtaa
    12181 ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt aaaattcact
    12241 agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga agttgattat
    12301 tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag aactcaaatt
    12361 ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa ttaagataga
    12421 aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt tctaggtcaa
    12481 gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac tttgacaaca
    12541 atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc gccaagaaaa
    12601 gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca agctcttttt
    12661 tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga aagttgaata
    12721 aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca gcactgtaat
    12781 tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca aagttgctaa
    12841 acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc ttactgatca
    12901 gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag cgaagtatac
    12961 ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat gataaaatga
    13021 gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct agtttcggtg
    13081 aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca aaaaggatat
    13141 tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg atcgtagtga
    13201 ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag agatgatcca
    13261 tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga tggaaatctt
    13321 ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct gtgatcaaga
    13381 atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt ccgatcagat
    13441 ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt tctctaggtg
    13501 cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc ccattccaaa
    13561 accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc attgttaatg
    13621 tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca gctagatttc
    13681 cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct agtcccgaaa
    13741 acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt atgaatggca
    13801 taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc actaaatttt
    13861 ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata cctattttcg
    13921 aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg tcattttata
    13981 tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt ccaatccctt
    14041 ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat atcaacgatc
    14101 cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat tgtatagaaa
    14161 ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa gatcgatgca
    14221 aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa agaagccatc
    14281 atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac aatggcgatc
    14341 agtggagaga ataaactact taatgcgaat agcactgcca ctactactga agtcaaacca
    14401 gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt attcgatgta
    14461 ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt atccattttt
    14521 gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa aatacctgtg
    14581 cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa actgaatgcg
    14641 atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga ttgcatgcct
    14701 tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt acctaacgaa
    14761 ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc aaggactgtg
    14821 gtcgctacga taccgataag aatagcccca cggacatttt ttacaactaa aatggctgtc
    14881 agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc taacgcaggg
    14941 acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac agagctcgaa
    15001 atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc gacatacgca
    15061 acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat cgcccgaata
    15121 atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca aataaacacc
    15181 attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa agtgaaaaat
    15241 gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa tcccattacc
    15301 agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa gggcatacct
    15361 gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa gaatgtcgtt
    15421 actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc gaaaaacttt
    15481 tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat aataaaaaat
    15541 aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt cattcgcttt
    15601 tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt tagcttatgt
    15661 ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt gctcttgtta
    15721 attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg acccttattg
    15781 aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga ttcgatgatt
    15841 acatctaaaa caaaagagac tctgaaaaaa gcaatcaatg ccggtcatca tgtcagtatt
    15901 gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga attaacaaca
    15961 ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg ggatggtgaa
    16021 agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa aaagcagcta
    16081 aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag ttttgatttc
    16141 tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct ttctcctaaa
    16201 aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt tgcaggagct
    16261 gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac atggggtgga
    16321 cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg cgtgcaagag
    16381 attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga cgagcataat
    16441 gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg tactgaccaa
    16501 ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg tttagctgtt
    16561 tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa agaggttgtg
    16621 acaatttttg tcacaacct
//
LOCUS       DACDYM010000056        16374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDYM010000056 DACDYM010000000
VERSION     DACDYM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.056"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IWM59_001895"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IWM59_001895"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9979923.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IWM59_001896"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IWM59_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9979924.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IWM59_001897"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IWM59_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9979925.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IWM59_001898"
     CDS             complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IWM59_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9979926.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IWM59_001899"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IWM59_001899"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9979927.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IWM59_001900"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IWM59_001900"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9979928.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="IWM59_001901"
     CDS             complement(8052..8522)
                     /locus_tag="IWM59_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9979929.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="IWM59_001902"
     CDS             complement(8568..8993)
                     /locus_tag="IWM59_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9979930.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="IWM59_001903"
     CDS             complement(9060..9329)
                     /locus_tag="IWM59_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9979931.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="IWM59_001904"
     CDS             complement(9340..10935)
                     /locus_tag="IWM59_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9979932.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IWM59_001905"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IWM59_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9979933.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="IWM59_001906"
     CDS             complement(14488..15048)
                     /locus_tag="IWM59_001906"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9979934.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..>16374
                     /locus_tag="IWM59_001907"
     CDS             15401..>16374
                     /locus_tag="IWM59_001907"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9979935.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
BASE COUNT         4317 a         3661 c         2923 g         5473 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aata
//
LOCUS       DACDYM010000057        16354 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDYM010000057 DACDYM010000000
VERSION     DACDYM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.057"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(195..773)
                     /gene="pgsA"
                     /locus_tag="IWM59_001908"
     CDS             complement(195..773)
                     /gene="pgsA"
                     /locus_tag="IWM59_001908"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9979936.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(982..1866)
                     /locus_tag="IWM59_001909"
     CDS             complement(982..1866)
                     /locus_tag="IWM59_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9979937.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(2644..3930)
                     /locus_tag="IWM59_001910"
     CDS             complement(2644..3930)
                     /locus_tag="IWM59_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9979938.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(3923..5191)
                     /locus_tag="IWM59_001911"
     CDS             complement(3923..5191)
                     /locus_tag="IWM59_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9979939.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            5443..5904
                     /gene="mscL"
                     /locus_tag="IWM59_001912"
     CDS             5443..5904
                     /gene="mscL"
                     /locus_tag="IWM59_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9979940.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(5963..6409)
                     /locus_tag="IWM59_001913"
     CDS             complement(5963..6409)
                     /locus_tag="IWM59_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9979941.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(6502..6747)
                     /locus_tag="IWM59_001914"
     CDS             complement(6502..6747)
                     /locus_tag="IWM59_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979942.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(6862..7440)
                     /locus_tag="IWM59_001915"
     CDS             complement(6862..7440)
                     /locus_tag="IWM59_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9979943.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(7517..8047)
                     /locus_tag="IWM59_001916"
     CDS             complement(7517..8047)
                     /locus_tag="IWM59_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9979944.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(8051..8911)
                     /gene="rlmB"
                     /locus_tag="IWM59_001917"
     CDS             complement(8051..8911)
                     /gene="rlmB"
                     /locus_tag="IWM59_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9979945.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(8904..9299)
                     /locus_tag="IWM59_001918"
     CDS             complement(8904..9299)
                     /locus_tag="IWM59_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9979946.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(9296..10708)
                     /locus_tag="IWM59_001919"
     CDS             complement(9296..10708)
                     /locus_tag="IWM59_001919"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9979947.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(10705..11241)
                     /gene="cysE"
                     /locus_tag="IWM59_001920"
     CDS             complement(10705..11241)
                     /gene="cysE"
                     /locus_tag="IWM59_001920"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9979948.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(11563..13023)
                     /locus_tag="IWM59_001921"
     CDS             complement(11563..13023)
                     /locus_tag="IWM59_001921"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9979949.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(13193..14350)
                     /locus_tag="IWM59_001922"
     CDS             complement(13193..14350)
                     /locus_tag="IWM59_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9979950.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(14384..15754)
                     /gene="radA"
                     /locus_tag="IWM59_001923"
     CDS             complement(14384..15754)
                     /gene="radA"
                     /locus_tag="IWM59_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9979951.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(15817..16344)
                     /locus_tag="IWM59_001924"
     CDS             complement(15817..16344)
                     /locus_tag="IWM59_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9979952.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         4336 a         3587 c         2936 g         5495 t
ORIGIN      
        1 tttatttaaa aagagggcaa ggagctgtga caaaaatctt gtcacagctc cttgttccaa
       61 ataaacagtg ttctcctgcc aaaacctcgg tcataagtca caatatacat aaaacatgaa
      121 aagctgtttt ttgaaattat cccttgtgac tcagagttag acggcaggct cacaacctct
      181 ccactaaacg atacttacat tgatcctttg aacacaccgc tgtttttgat gaagtaatcc
      241 acacctgaat agatagtaaa gatcagacaa gcataaagca tgatttgatc tactggaata
      301 ccgatcaggc tgaatgggat attattgata aacagcaaga taatagcaac catttgtgta
      361 gccgtcttga cttttcccgg ccaagctgca gccatgactt ctccatgttc gacaagtagt
      421 aaacgcaagc ctgtgacggc taattcacga cagacaatga tcgctactac ccaaccaggg
      481 gcctttcctt ggtcaaccaa catgataaaa gcagtcataa ctagcatttt atctgccaac
      541 gggtccgcaa acttgccaaa attagtgaca aggcctcgcg cacgggcaat ttttccatct
      601 aaccagtctg tgatactagc taaagcaaaa ataactgcac ctactagctg tgtgacagcc
      661 agtgtggtgt ctcctacgtt aaattgtccc caatctaaag gtaaacctac aaccaaaatg
      721 aaaatcggga tcatacaaat tctaagtaca gttaatttat ttggtaaatt cacactatcg
      781 actcctttct tccctattct acgtgactat cagcgaagtt tccaccgata ttatttactt
      841 ttccaaatga atttattcaa aggagtattt ttattgaaat tgatcttttt gaattaacga
      901 agaaggaccg cgacacagat ttgtcacgat ccttcttttt tatacctaca agtaaacaga
      961 tgcgtaaact aatttatacg atcattctgc gtattgaaga gtaaggttca cattttttgg
     1021 actctgattg ttttcccctg gattaactgg tacggattct ccattcgctt tgatcgttgc
     1081 attgcttgct gcaccaatcg tgatggtcgc atttgttgct gtttccggta acacggtact
     1141 ctgtgtttca tttgctgcca gtgtcccttg ataaacatag gcattgtcta ctagcacacc
     1201 taaccataca cgattgacag cagtaaattc aagggttaca ggcttggtag cgtctgtgat
     1261 atttactgcg atggcactgc cagaatcttc tcctgtagct attgccattt tcttttcttc
     1321 acttgatttt gttgttgatt ctactgtaga agactctttg gtctgcgtgc tttgctctga
     1381 ggaagaggac tcctcgactg atccttctac tttaagcgaa gaagctgtgt ctccgattat
     1441 tggatcagct tgacgatctt gccaagtcat atagcccacg acaacgacaa tcgccaaagc
     1501 aaccaatcct aataaaatga caggaagact tgtccaaaag cgacttggat gcgattcttc
     1561 ctgatgcatt gcttttctag aagtacttac tgtttcaggc tgcggacgtt cagggaccga
     1621 tcctgtcaat ttgcttttcc catcaaaaac atcgactaaa tggtctccat cttctcctac
     1681 agcttcagcg tattgccgaa taaatgcccg cacgtaaaat ttaccaggca attggtcaaa
     1741 atcatcattg tcgattgctt ccaaataacg tttttgaatt tttgtaatct gctgtaattc
     1801 atctaaagat atttttttat tcaggcgtgc ttgccgtaat ttttctccga tggtttcaaa
     1861 agccactcgt tcatcttctc cagtctgttt gattctccga agcatctgct gttgcaacga
     1921 aagacgttat gtttgggtgc tgtgacaaaa gtcatgtcac agctcctttt ttccgaataa
     1981 acattgttct cctgccaact cctcggtcac aagtcacaat atccaataaa tataagaagc
     2041 tgttttttga acttgttcct tgtgactcgg agttaaacgg caggctcacg acctctttct
     2101 taaacggtgt tcaatcagct gttcctcggt attagctcaa aaaccggcaa aatataagga
     2161 gatatttttc cgtatgtttc tctaatactc aggtcaaaac gctgatttca caacctcttc
     2221 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacatt
     2281 aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
     2341 tcttggtaca cggtgttcca gaagtagctt cttcggcaat aagccgaaat atcacaaaaa
     2401 tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca
     2461 acctctttga ttacttcgtt tgattttatt ttaatataag ttgattaaaa gagagcatga
     2521 tttgttttat tttctcacta atttttccaa cacaactaag aataccataa aagctgattt
     2581 tggaggaggt tcctactaca agtctaaaac gaaatttaag acttttcctc ataaaatact
     2641 ggtttatttt ggtctcatgt aaaactcgct gaaggcctct tcacttataa atgcttcccc
     2701 agctgccagt acgtctttca tttggatcga atcgatgatt tcaggaagat caaataaagt
     2761 tcgatcgcca aataaacttt gcgtaaattg attggcaata tattcgatcg aatttaatga
     2821 ttggaaatat tgtccaagca ttttcttctt cagcaaatcg agatttgttt ctgacacttc
     2881 cggatcatca gcgaatccaa gaatgatttc tttgactttt tctgctgctt tctctggttc
     2941 gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga tccaagctga attcaaaacc
     3001 aaagctgtca tcgataattc cttgattgta catcgctaga tagtttcgag aagtattgcc
     3061 taaaatcatt tggaacaaca gattgattgc tgttttatag cgtaacagtt ctgttccttc
     3121 ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat ttatctcgtg taatggctgc
     3181 ttccaatgca ctttgtttga tgatttcttt tgtgttttca gggaaataac gtacaatttc
     3241 ttgctttgga gggaaatttt ttgcttcttg gttttcccga ataagtttca tcaatttctc
     3301 aggctccatc ttaccaacta caaataaaac catattgctt ggctgataaa acgtccgata
     3361 gcacgtatac aagtcttgag ctgtgatttt gtcgatactt tcaaccgtgc ccgcaatatc
     3421 gatatgcaat gggtgtgtag gatacaagtt atttagtatg ccaaaaaaca tccgccagtt
     3481 agggtcgtct tcatacattt ggatttcctg accgatgatt cctttttcct tgtttacagt
     3541 ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga gtcgtcagat tcttttctac
     3601 ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa cttgtgaaag cattggcgga
     3661 agctccttgt ttcccaaata attgaaagac atcaccatct tctttttcaa acaacttatg
     3721 ttctaaaaaa tgggcaattc catctggaac tttcaccttt tctttttcac catagggaat
     3781 aaattcgtta tcgatcgaac cataattcgt actgaacaaa ccatatgtct tgtgatagtc
     3841 atttttaggc aacaaataca cggtcaaacc gtttggcagt acttcatgat aaagtgtttc
     3901 attgatttgc tcatattctg ttttattcat ttgcatttcc tcctgctaaa aagaagattg
     3961 cctgcaattc gatttgctca gctacttgtt gtatctcttt gatcgttacg ctctccatac
     4021 gtttcaacca ttcttcatca gataattttg tttccggcag ccagctgtcc aaaaaagaag
     4081 cttcgattgc cgcttgcgga gagtctagag aaagcagata ttggtttctc agcattgctt
     4141 ttgtctgaga cagttccaac tctgttattt caccatttcg caaactttct aattgctcgt
     4201 gaatcagatg aagtacttga tttcggttct tttcatcgat accggtttgg acactcatga
     4261 atcctcggaa cgtatccacg ctactagagg cataataagc tagactttct ttctctctga
     4321 cattcatgaa aagtttagag tgaggaaaac cgccaaataa accgttaaag accattagcg
     4381 caaaacgttc tggttcatcg taatacacat ttgtctgata agctaggttc agctttgctt
     4441 gaacgattgg ctcacgtact tgtcgttcct tcacgatatt cacttgaggt tgggtataaa
     4501 acagatctgg ataaactgtt tcccgcgact caaatggtaa ttgcccaact gcttcagctg
     4561 ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat ttgatcattt gccatcattt
     4621 cttgatagta ggacgctagc gaactagcag tgatcttgtc caaatcttcg actacgccca
     4681 accctgggat cttttgttcc gggctgtttt caaaataaag ttcttgcaag gcataggaag
     4741 caaaagtttg tttatcttcc ttcaaacttt ccaaatatag acgcagattg ttcttttcca
     4801 agtcaaacgt caactgatca aactgatgat ttttaatatt tggataaaat aaaacctctt
     4861 ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt gacatattca ccattcacaa
     4921 aactgatccc tgcgtttagc caatgtaaat ttcctttttt tctaactgat aatccaaagc
     4981 ttgcgccata aagctctgca agttgcgcac ttaactttgt ctgatccgga taattcaaac
     5041 tatttgtttc caacatgctg ctgagaagtg aacgtttcgt gatgacttct tgttgcagac
     5101 gcgcagtaaa acggataaaa atccggatcg ttttgtattg ttttgttggt aagacatgaa
     5161 gattcacgcc ttctgccaat tgatacacca tatttatatg ctccttttca cagttgattg
     5221 cccgcttact cttttaatgg gtcaacctgc cttttttact tattcctctg cttgaaattt
     5281 ataagttcag gcaattcgcc gttttttcta aatgatgtta cactttatag tatagcatag
     5341 atgtttttta tcaaaagaaa cagacggcta aaagcgtata ttgttttttt attttttatt
     5401 tttcgatata ctaaagacaa ataaaagaag ggagggtcat tcatgatcaa agaattcaaa
     5461 gagttcatca tgcgtggaga tgtcttggat cttgctgtcg gcgttgttat tggtagtgcg
     5521 tttacaagca tcgtaaacca agtggtgcaa ggcttgatca caccactaat aggttggttt
     5581 gtcgcaatga tcacaggtac ttctgattta gaaggagcac tttcaattct tgactggtcg
     5641 ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta gtgctatcat tacatttctg
     5701 atcactggtt ttgtcttatt cttagttgtc aaagcggcaa accgtgcgaa gaatttacgc
     5761 tccaaagaag agcaagtgga agtggaagaa actcctactg cagaagatta tcttagtgac
     5821 atacgcgatc ttcttgccaa acaagtggca gaagacaata cacatccagt agagcaaaca
     5881 aatctttccg atacttctaa ataataaaga atctaaaaaa aagaaagccg attttcagtg
     5941 tcggctttct ttttttagat tcttaacttg ctacttgttc ctttttcatg tcgatatgtg
     6001 gaatatcatc ttccaaatac acatctgaga ttgccttgaa tccaaacgac tcataaaaag
     6061 cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt ttctggatca
     6121 tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc tttacggttg tcctgatgaa
     6181 caaggacacg tccaaaatgg atactctccg cttcttcata tactcgcgtg taggcaagga
     6241 tcttcccatc agtatcttga aaaaacgtat gccaagcttg tccatcaatg tcatcaactt
     6301 cttgataagg acactgttgt tccacaacaa aaactgcgat tcgttctttg acgatcgcgt
     6361 aaaactctgc tgttgacaat tcttcaaatt tctttacctg atattccact aagcaaacgc
     6421 cccctgcttt tttattctta gcataccgat tcgcacacaa aaaagcaaac gaaccttacg
     6481 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
     6541 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
     6601 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
     6661 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
     6721 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
     6781 tttttaaaat tttcaagttt accataaaaa aaaaagaaat gcaataaaaa taaataagaa
     6841 gctcattaag atttttctaa attattcctc attataaaat ttcaagtgtt tttttatttt
     6901 gagttttact ctcccgtaag cgctcattac ttgcttgtga tccattgcta aacattcagc
     6961 catgtctaca aaatcaacgc cttgcatata ataatgaaaa attttagctt ctatttttga
     7021 aaactggatt tcgcaatttt cgattgcttc ttttaataaa agatgctgaa tcgctaaagg
     7081 ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct gtctctattt tttggtcgat
     7141 tgagacttca tctttttgat cacgacgctt cagcgcgttc tgcttcctca acagagaata
     7201 gatttgattg cttataatgc ttttataata acttccaaag ctaacacctt tgcttttttc
     7261 gtaagttctc aatgcctgat ataaagcgat ccttgcttct tgttcccaat cctcagaaac
     7321 ataatttctt aaataatagg tgtttttcaa acgtagtaaa agtggaaaat atttttgata
     7381 cagggcagca aatgcttctt cgtcaccatt acgtgcagcg cgcaataact cctcagacat
     7441 aaaataattc ctccataaat tcgtttatgg aggaatctta tcatcaatga ttcatcgaac
     7501 tgattagaac gatatgttat ttattttttc tcattaaatc gtccaatttt tgggacaatt
     7561 tttttaactg ctcttcattc catggagaat tacgtcggaa atcaataaaa tgcatatctt
     7621 tattttcttg ggctattgtt ttttccgttt tttttatcgt cttatataat tcataagcag
     7681 aagttcgtaa tgctccttgg gaaaagatca tccactgttc tgctaaatcg cttgtcgcca
     7741 ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat tctctcgata taactgtcag
     7801 ccgtttcgtc ttctttcgta aaaacaacat ccaactggta tttcttataa ttttgttgga
     7861 tgcccggaac tagttgggca tcgaagacaa cgatgacttc cattccttca tatttcgcat
     7921 aatttgataa acgatgtaaa agagcttctc ttgcatccgc cattttgtcc tgctttttaa
     7981 gctggacaag ttccggccac gaaccaatca tgttgtagcc gtctacaatc aacagctgtt
     8041 ttttcatggt ctactccccc tgacgttggg aaaaagcctg atacataagc aaaccagcag
     8101 caacactggc atttaagctc tggacgtgcc cggtcatcgg gatcgtcagc aattcatcca
     8161 tttctttttt caatcctgga ctcattcctt tgccttcatt accaatgact aaagcaattg
     8221 atcctttggc attccatttt tggaagtttg tgccggtcat atctgtgcca aatacccaaa
     8281 atcctgtttc tttgagctgt tgcacagctt gtaccaaatt cgtcacgcgt gcaacaggga
     8341 catgttcgac tgccccagtg gaagtctttg ttacgatagg tgtgattcca actgctcggt
     8401 gttttggtat gatcacacca tcgacgccac ttgcgtctgc tgtccgtaaa atcgacccaa
     8461 aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa ggggtcttct tttttctctt
     8521 ttgttcgttc aatcagttgg tcaagggata aatattcata aggtgtaatc gcaagaacga
     8581 ctccttgatg cacgccgtga tcgctcaaag tttctaattt ttgtttagga acccatttga
     8641 ctggcacagc ttgttcacgt gcgatttctt tcagttcttc tactttttct cctcgtgaat
     8701 cttcttgaag aaagagttta ttccctcgcc cttgttgtag cgcttctact gcggcatggt
     8761 gcccaaagac gaaattatct tctaatgctt cttcattttg atcagaaact gactgatttc
     8821 gtccagtttg ctttttattt gaatatctat tttgtttttt gtcttcaaat ccacgtttcg
     8881 attgatgttt gtttgttctt tctttaccca tttttttcac caactttctg aatacaccag
     8941 cggatcaatt cttccatccg ctcggtttgt tttgtcaaat gcagatagcc catgagcgct
     9001 tcgaaccctg ttgcgacccg atacgtcgta acatcagcat tttttgcgct cgtatggctt
     9061 ttagcatttc ttcctctgcg ataataaagt tcttcttctt ctgtcaaaag atgttcttcc
     9121 agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa cataattcgt tgccgcacga
     9181 tgcagcttat tgggttttgt ttctccttga tcaatcaggt ggtcacgaac atagatttcg
     9241 taaatcgcat ctccaacata agccaaggct aaaccgttca attgtgtgta atctctcatg
     9301 cttctcttct ccatcgggtt ccttgcggtg tatcttctaa taagatccct ttttctttca
     9361 acaaatcacg aatttcgtcg cttcggacaa aatcacgatt gcttcttgct tgatttcgtt
     9421 cttcgatcaa ttgttcaatc tcttcatcta ataactcttc tgctgtaaat aaaataccga
     9481 agatcgaaag ccattgcgag aataaagctt ctccttgtgc caagacagcg gcagaaactt
     9541 tcggctggtc tagataagtg ttcagccact tagcaagctc atagaccact gtgatcccat
     9601 ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg ctgttctaaa ttttctaaca
     9661 aagcgatttt atccgcatca tctgccaaat tctctgaggc agtttcttgg cggaatttca
     9721 aattgtcaaa cgtattcttc agtcgttgta aattggtggc tgcttctttc atcgtcgttt
     9781 cactgtagcg gatcggacga cggtattgtg tggtcgccat gaagaaacgc aagacttgtg
     9841 gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt ccctagagat ttactcattt
     9901 tttcatcgtc ttcgccaatc gttacatagc cattgtgcat ccagtaatta gcgaattttt
     9961 tccctgtttt cgcttcactt tgtgcgattt cattttcatg atgaggaaat tctaaatctt
    10021 gcccgccacc atggatatcg atcgtttctc ctaaatgttt tgttgccata acggaacatt
    10081 caatatgcca accgggacgt cctttgcccc atggtgaatc ccaagaaatc tcgccaggtt
    10141 ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt ttgctgctgt tcttcccctg
    10201 tacgctgact tgctccgact tccagttcat cgatggattg gtcacttaat ttcccataat
    10261 tttcaaattt acgggtacgg taatacacat cacctgaaga ttcataagca tagccttttt
    10321 caaccaacac ttgaataaat gctaaaatat ctggcatatg atccatgact cttggatgca
    10381 gtgtagcagg ctcgacattc aacgctttcg tatcttcttc aaatgctcgg atgaaacgat
    10441 cagcaacttc tggtgctgta atacctaatt cgtttgctgt acggatgatt ttatcgtcta
    10501 catccgtgaa attcgatacg aaatcgactt tatagcctcg atattcaaaa tagcgtcgaa
    10561 ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg gatataatta tacacagtcg
    10621 gtccgcagac atacataccg acatgtccct tttgtatagg gatgaattct tctttttttc
    10681 tggtcaatgt attgtagatt tttatcattc tgatctcctc actttttctc ctttgattcg
    10741 aacgactttt gctggtactc ctaccgctgt agcgtcgtct ggaacatctt tcaacacaac
    10801 ggcacctgca ccgattttgg cgttttttcc gatcgttacc ggtcctaata tctgcgcatt
    10861 cgcagagagc atggctcctt gtttaacagt tgggtgtctt ttacccgttt cttttcctgt
    10921 gccgcctaat gttacgccat ggaataaaat cacatcgtcc tcgatttctg cggtttcgcc
    10981 aatgacgatt cccattccat ggtcgatgaa cacgccagtt ccaatcgttg ctcctggatg
    11041 gatctcaaca ccagttagga aacgccagaa ttgagcattg atcttcgcca ataaaaagag
    11101 ccggtgctga tataaaaaat gagagaaacg atgccaaaac aaggcatgaa aaccagggta
    11161 tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg tttctctttg ctgcatcaat
    11221 cgctcgggta aaccaactca tgactttcca ctcctttcgt aagaatttta tgtaggaaat
    11281 catcttctta taaagaaaag aagcgccttt taaataagtt tccttatcaa aagacgcttt
    11341 ccgcgtggtt ccacttttct tcataatcaa tgattatctc tgacagctaa cgcctgttat
    11401 acgttcttgg ctactttgtt tcctataaaa cagtttttcc tgttttctgt gtttcgccaa
    11461 gaccactccc agaggcattt cggcaaccat tcattgatac cttgcaccac ccggtatctc
    11521 tcttgtaatg atcagctgct tacttttttc tgttcatcgt gtttagttca gtacttgatt
    11581 taaatgtgct aatgattttt cacgaccaag caattcgatc gtttcaggca attctggacc
    11641 atgcatttgt cctgatacag ctacacggat tggcatgaaa agattttttc cttttacgcc
    11701 agtttctttt tgaacagctt tgatggcagc tttgacagtt ggagcatcta ctacttccat
    11761 ttgttctagc tgtgctttga atgcgtggag aacagtagga actgtttcac cagcaagtac
    11821 ttcttttgct gcttcattca atacaggatg ttcattgaaa aatagcgtag acagatcaac
    11881 gatttctgca gcatagctca tttgaggttg atacaggcta acgatcttct tcacccattc
    11941 gattgtcttt tcatctgggt tttctgctac acgtccatca gcgattagat aaggcagaca
    12001 catgtcagta aatgcatcta gatccattgc tttgatgtaa tggttgttta cccattgcag
    12061 ttttttcgca tcgaaagctg cgggtgattt acttaggcgt tttggatcga acattttgat
    12121 caattcttgg tgttcgaaga tttcgtcttc gcccacaggt gaccagccca ataatgcaat
    12181 aaagttgaac attgcatctg gtagatagcc taattcgcga tattgttcga taaattgcaa
    12241 gattgattcg tcacgtttgc ttagtttttt acctgtttca ctgttgatga tcaatgtcat
    12301 atgtccgaag gttggtggtg tccagccaaa tgcttcatag atcatcagtt gttttggtgt
    12361 attggcaata tggtcatctc cacgtaaaac gtgagtgatt ttcatcaaat gatcatcgac
    12421 cgcaacagca aaattataag ttggcatacc atcgcgtttt tgtatgacaa agtcgccacc
    12481 gatattatcg gattcgaacg aaatagcgcc tttcaccata tctgtaaatg cgtattctgt
    12541 attgcgaggc acacggaaac gtacaacaga ttctagacct tgtgcttctt tttctgcttg
    12601 ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga ggcatttctc ctcttgcgcg
    12661 ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa tagcatttgt atgctcgatt
    12721 gctagcaagc agctgatcaa tcagcggttg atagatttct ttacgttcag attgacggta
    12781 agggccgtat tctccaggat tttcaggaga ttcatcccaa ttcattccca accaagcaag
    12841 gttttccaac tggctttttt cgccatcttc gatattacgt ttttggtcag tatcctcaat
    12901 acgaataata aagtctccat cattgtggcg ggcaaacaaa taattgaata atgcagttct
    12961 tgcatttcca atatgcaggt gcccagttgg acttggtgca tagcgtacgc gtactttcgt
    13021 cattttcttc ctcttctttc aaactagact ttgactaaca gcaaaaattc cgtcagttac
    13081 ggcttctcaa atatcattta tttaaagcca ctcagcacaa attttacctt ttctaaggta
    13141 aatagtaaag ccaactggtt gaatttcctt accagttggc tgaatttctt ttttattttg
    13201 attgtttagt ctttttctct gtctcttttt tattgtcgat tcctcgtcct gcatgtgcag
    13261 gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc acttgttaca acgacttcga
    13321 tacgttcatt catgtaatgc tgtccatctt ctacgacaac catcgtgccg tcatcaaggt
    13381 aggctactcc ttgttggcgt tctgtcccgg cttttactac tagaacattc atcgtttcac
    13441 cagggatgac gactggtttt acggcatttg ccaacgcatt gatattaaga acaggtacat
    13501 tttggaattc agagacttta ttcaagttat agtcattggt tacaatgaca ccatctaaaa
    13561 gcttagccaa tttgatcaat ttgctgtcta cttcactgat atcttcaaag tctccatcgt
    13621 acatttcgac agatataccc tcttcttttt gcaaggcatt taggatatct aatccgcgac
    13681 gaccacgcac acgtttcaaa ctatcccctg agtccgcgat gtattgtagt tcgtaaagaa
    13741 caaaattagg gatcatcaag gttccttcta agaatccagt ctttgtaata tcataaatcc
    13801 gaccgtcaat gatcacgctt gtatccaaga ttttatattt atggaaattt tctcctgatt
    13861 ttcgttctaa cagttgactc tcaacttgac tagctgtttc ttgctcgatt tttttgcttc
    13921 gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt tcccattctc aatcccagat
    13981 agcctaagat gatcatcagt aaaatcggca atacactatt gacgaatgga atttccatat
    14041 tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc gatgatcgca ccgatcgcac
    14101 cgaagagaag ataagtcaag ctgacctcac ttagtttttg ttctacttgt ttgataatag
    14161 ctgagatatg tttggccaac agcaaagata ataaaaagaa aataagtgcg cctaacaggc
    14221 tgttggtaaa tgtattattc agccatccgt ttgactgttg accgattgcc tgccaagctg
    14281 agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat catcagcaaa gtaatgacac
    14341 gtttttgcat cgtttttcct ccgtttcgac aatcatttat tagttagcgg aacacacgct
    14401 tcaacgtttc agccaaagtt gctactccaa ctatctcgat tccttttggc gcttcccagt
    14461 tacctaagtt gtttttaggt aggtatactt ttgtgaagcc gagtttttgg acttctttga
    14521 ctcgttgttc aatatgactg acacgtctga tttctccagt cagtccaatt tcaccgatga
    14581 aacattccgt tggctgtgtt cctttttctt tatagcttga agcaatactg acagcgatag
    14641 ctaaatcaat cgctggttca tttaatttta ccccacctgc agcttttaag taagcatctt
    14701 gattctgaag aagcaaccca gctctttttt ccagcactgc catgatcaga gatacccgat
    14761 tgaaatcaag acctgttgtt gttcgtttgg catttccgaa catagtaggt gtgactaatg
    14821 cttggatctc taccaaaatt ggacgtgtcc cttccattgc cacaacgata gcagagccag
    14881 ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg attagcgact tcttccaatc
    14941 catgctcatg catttcaaaa atcccaatct cattcgttga gccaaatcgg ttttttactg
    15001 ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa atataacacc gtatccacca
    15061 tatgttctaa cattcttggg ccggcgatcg agccttcttt ggtaacatga ccgacgataa
    15121 aaatggctat cccatttgtt ttagcgattt tgagtagttc tgcagtggtt tctcttactt
    15181 ggctgacact tcctgccaca cttgtgatgt ctggctgcgt catcgtttgg atggaatcaa
    15241 tgataacata atcaggggag atatgttcga tggcacgact gatttcgttc atatctgttt
    15301 cagcgtatag ataaaattct gtgttgatac ttccgaggcg ttccgcgcgt aattttatct
    15361 gctctgcgct ttcttcgccg gatacatata agacttttcc gcctatggct gctaattgct
    15421 gagaaacttg taacagcaaa gtggatttcc cgatacctgg atcgccacca atcaagacca
    15481 tagaccctgg caccacacct ccgcctaaca cacggttcag ttcttctaat tttgttttga
    15541 tccgaggttc ttttttaggg acgacgtctg cgatttttgt tggttttgct ttttctcctg
    15601 tcaaacttgt ccgagttctg cggtccgttg tatcttgttc gatttcttcg accatcgtat
    15661 tccattttcc gcaatttggg cagcgtccca agaacttagg cgacacatac ccgcaatttt
    15721 ggcaaacaaa ttgtactttt gatttttttg ccattgtacc gcctcctttt atcctaatca
    15781 tgagacatta aatcaagctt aagtatcttt aacgttttat ttactggaag aaccaaatcc
    15841 gccttgacgt tgagcagtcg tttgatcttg atctgctttt aaaaacggca aaaagatccc
    15901 ttggccgata cgctcgcctt ttttgatcac tacatccttg aaaccaaagt tggtgaattg
    15961 gaacatgata tggccttcat ttgcctcatt attgtaataa tcactgtcaa tcacacctac
    16021 tccgtttgtc aacactaaaa accgcttcaa cggattgctt gaacggttag caagctgcaa
    16081 aaattcatct tctcccatgt aagctttgat tccagtaggc acgagggttg gtttcaattg
    16141 tttttcattt tctgatacat cttttttcaa tccatctgcg actgtttcca accccttttt
    16201 ccagcctgct ttccagatac ttggaatcac gacctcttct gctgcttcaa aatcatagcc
    16261 agcagcatga tgtgtagcgc gctgcggtag attgatattt tgatctttat atttagaaat
    16321 aacttcaaat cctctttttc tcaccggttt tcct
//
LOCUS       DACDYM010000058        16324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDYM010000058 DACDYM010000000
VERSION     DACDYM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.058"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(109..765)
                     /locus_tag="IWM59_001925"
     CDS             complement(109..765)
                     /locus_tag="IWM59_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9979953.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            958..1236
                     /locus_tag="IWM59_001926"
     CDS             958..1236
                     /locus_tag="IWM59_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9979954.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1255..2463
                     /locus_tag="IWM59_001927"
     CDS             1255..2463
                     /locus_tag="IWM59_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9979955.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2611..2976)
                     /locus_tag="IWM59_001928"
     CDS             complement(2611..2976)
                     /locus_tag="IWM59_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9979956.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3123..3896)
                     /locus_tag="IWM59_001929"
     CDS             complement(3123..3896)
                     /locus_tag="IWM59_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9979957.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4070..4930)
                     /locus_tag="IWM59_001930"
     CDS             complement(4070..4930)
                     /locus_tag="IWM59_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9979958.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4930..6348)
                     /locus_tag="IWM59_001931"
     CDS             complement(4930..6348)
                     /locus_tag="IWM59_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9979959.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6405..6854)
                     /locus_tag="IWM59_001932"
     CDS             complement(6405..6854)
                     /locus_tag="IWM59_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9979960.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7299..7562)
                     /locus_tag="IWM59_001933"
     CDS             complement(7299..7562)
                     /locus_tag="IWM59_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9979961.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7661..8956)
                     /locus_tag="IWM59_001934"
     CDS             complement(7661..8956)
                     /locus_tag="IWM59_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9979962.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKECPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8953..10224)
                     /locus_tag="IWM59_001935"
     CDS             complement(8953..10224)
                     /locus_tag="IWM59_001935"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9979963.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10389..11258)
                     /locus_tag="IWM59_001936"
     CDS             complement(10389..11258)
                     /locus_tag="IWM59_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9979964.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11550..13160)
                     /locus_tag="IWM59_001937"
     CDS             complement(11550..13160)
                     /locus_tag="IWM59_001937"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9979965.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14353..15693)
                     /gene="glnA"
                     /locus_tag="IWM59_001938"
     CDS             complement(14353..15693)
                     /gene="glnA"
                     /locus_tag="IWM59_001938"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9979966.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15721..16113)
                     /locus_tag="IWM59_001939"
     CDS             complement(15721..16113)
                     /locus_tag="IWM59_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9979967.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4658 a         3460 c         2775 g         5431 t
ORIGIN      
        1 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt ttccgtttgt
       61 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttgttcatc aaaagataat
      121 attgtcttct aacaagtcca tattcaaaat aacgatatga tggtctttaa cggtgataat
      181 accatctttt ttcagttgtt ttaaaattct gtttacactg ctagcagttg agatgccaca
      241 aaatttggcg atttcttcat tcgtgatcga aaaatcgatc agcatccctt cttctgtccg
      301 cacaccgaat aaatcatata attcgtaaag ttgcgtacaa acagctccca gctttccgtt
      361 taatagcatt tgctgcattt ttttcatcga ataaagcaga cgatagcggt aatactcttt
      421 tacatagtgt tgtagctttg ggtcttggtt gatattttgc caaaagcgca cacgattgat
      481 tcgataaaac tctgcatatt ctgattcaac tcgaatatta tacggcgaat cgatgaattg
      541 tgaatattcg tcttttaaaa gagagacgat ttcaagatct ttgatatagc gaaggttaaa
      601 ttctctccca tcatttgaaa taacacttgc ttttaccgtt ccctctttca agatatacgt
      661 atgggaatct tccatccctt cgaatgtcaa atatttatga tatcctttgc gtataatcgg
      721 aaaatcattt ttatccaaat attcctttaa gactctacta tccaatgatg ctcccccatc
      781 caaactttca tctaaaaaga tcatacagta ctttaaaact ttttaaagtc caaaggtatt
      841 ttactaatga tattaacatt tgttaagtac ctttttccat cacttatgcg atttttttcc
      901 ttttttttat tcgttatact gcagaagtaa aattttttaa gaatagaggg aaaaaacatg
      961 aattactgga tgaacacaat cattaatcga ttagaaacag catatcaaac aagatttgat
     1021 atgaaagctt ctttagtctt tttaaatgat gcgtatcaaa actcgatcga attgatcaaa
     1081 gcagtggatg agaatccaac aaacgaatgc gaagaatttt taaatctgtt tatgtctaca
     1141 agagatttat ttattcgcca acttgttgat cgctatccat ccaattatca cgatgtagaa
     1201 gttcaaatac aaaaattaaa agcatattca gcttaagggg aatgtataac atacatggaa
     1261 aagaaattat ttaataaata ttttattgga atcacattga tcaattttat tgtctacctg
     1321 atttattatc tgttgatggt catcatggca gtcattgcgc aagacacctt acacgccagc
     1381 ttgagccaag caggttttgc ttctgggatc tatatcatcg gaacattgct ggcacgctta
     1441 ttcatgggga aacagttaga actatttggt cggaagttta ccttaagagg cggcgcgatc
     1501 ttctatctcc tttcaacatt agcatatctt tatacgccaa ctatcggggc attgtattta
     1561 gttcgattct tgaatggatt cggctacggt acagtttcta cagctacaaa tgctatcgtt
     1621 actgcatata tccctgcatc taaaaaaggg gaaggcatca attattatgg attaagtact
     1681 agtttagctg ccgctgttgg gccattttta ggaatgattt tgctgaatat gactgatttt
     1741 cgtttcatta tctggttttc aattgtactg gtcttctttg ttacgattgc ctgccttgtc
     1801 tttcctgtaa aaaatattcc attatctgaa gaacaaaaaa tatctcttcg ctcatggaat
     1861 atcaatagtt ttattgaaaa gaaagcactt ttcattacag gaattgcctt tttgatgggt
     1921 ctttcttatt caagtgtttt atcattttta tcttcttata caaaagtaat ccacttagtt
     1981 gctgctgggt catttttctt cgtcgtatat gctttggtca tcacatttac acgtccattg
     2041 actggaagga ttttcgatgt caaaggagaa caatacgtga tgtatcctag ctatttattc
     2101 ttaacagcag gattattttt actgagtgta acgacgaata gtgtgacatt gcttatctct
     2161 ggagcattag tcggcctagg ttatggtacg tttatgtcaa atggacaggc tgtttgttta
     2221 aaaatcgtcc gcgaacatcg aatcagtatt gctttatcta cctactttat tggtttagac
     2281 ctaggcttag gcgttggtcc ttatttgatg gggatgctaa aatctcttac ttcatttaga
     2341 ggattgtatg tgattgccgg agtcattcca ttgatatgta cagcactcta tcttgttaat
     2401 ggccgaaaac atagtcatga agcagagggc gaattgattg aatcattgaa aaatgaaatt
     2461 tgatcttgat gaatagataa aaaaaaccgt tcagatctga aaataatctg agcggttttt
     2521 tgagtaaaaa tagtttatga ttattcttaa ttaaacatct atttattttt aaagctttca
     2581 tttatcgata agaatagttt aactgttgat ttattccatt ttccgttcat ctggtacgat
     2641 gccttgactt agcaaccact tgtggaagaa ccattctgcc acaccgatca atagagcagg
     2701 aatcaatact ttcatccaaa tatcgactgt atcattcata aaatatagat agagccacaa
     2761 gataactgca gctgaaccag catctgcgat cgtcgctgcg atgttcccta ctttacgtaa
     2821 gatcaacaga tcccctagca catagatcat gactacaata attaccccaa caatcgttga
     2881 atccataaag ctaacaccgt ataaacctgt cagcacgacc cacagaatcg cccagatcat
     2941 gattgccttc actaccaacg ccttcacatg tttcattttc tgcactcctt tctcctggat
     3001 aaaaaagtaa gattatctaa caagataacc ttttcattat tataccatcg gctgagagaa
     3061 gcgagcaaag ataaggtcct atctaaaaat gaggaagcca cttagaacat tcacgtaata
     3121 atttatccgt acagacattc gatatggtgt tcctttactt tcgcatactg ttcttttgtc
     3181 agttctttac ttgtcaccag ataatcgatt tcatgaaaag caatggcttg atgcatgaat
     3241 ttatgtgcaa ttttttgctg tgtaactaaa acggcaagcc ggtctgattg ttttctaaaa
     3301 gcttttttga ccattgcctc tccttcatga gtttctgtta ctccttggct gaaatctagc
     3361 ccgcgacaac tcattatcgc taaatccgca taatatgaca atgcatcgtt ataggctttt
     3421 gaaccgttga ttgttgctcg tttcggaaca attgcccctc ccaatacaga tacttttgcc
     3481 gttgtatatt cactcaacat agcagctgtg acgatgccat tcgtaatgat ctgcaaacca
     3541 tcaaatcttg ctaggtttct gactaagtgt aaagaggttg tactggaatc caagaataag
     3601 accatatcat ctcgaatcaa agaagaagct gtatctgcaa tcttacgttt ttctgtttct
     3661 tcttctaatt tggtgatctt catcaggtaa ggcacgtcaa tttttcgatt gtcaataagc
     3721 atggcgccac cgcgaactct acggatcaat ccactttttt ccagaaaaac aaggtcacgt
     3781 ttaatcgtgg cttcgctata atttactaac tcgatcaaat cggtgactct tgcaaattgg
     3841 ttgtttttta gataattcac aatctcctgc tgtctattct ccacttcatt caacataaaa
     3901 tcaccccatt tactttatat agtaacagga aaaaagaggt tgtgacagaa gtgattggct
     3961 tcgagaaata agaagaaatt tttcgaaaaa actacttctg aacatcgtgt gtggttaaga
     4021 gggtatgaca agactattgt cataccctct tgaccacaca tttttatttt tagtataaac
     4081 tagccttgcc tactgtgttc aaaatttcca ttttatgttt gaccacttct tgtacctttt
     4141 tattagctgc tggataaatt gcattcggct cgtacatcga tggattttca ctcaatattt
     4201 ctctcaaagc attgaaaaag acgcttttaa gatctgagct caaattcact ttggcaactc
     4261 cgtattttac tgcttcgctg acttctgcgt ccggattacc tgaaccgcca tgtaaaacaa
     4321 atggaagatc gatttgttgg ttcaattctt tcagtaagtc gatatttaat tttggagttc
     4381 tgtttttcgg atacaagcca tgcgctgttc caattgctac agcaagtgta tcgattcctg
     4441 tttctttcac aaaaatcttc gcttgatcag gatctgtata aatgattgtt tcagcacctc
     4501 cttcagcaga accattattc ccaatcgttc caagctcagc ctccactgag acatttaccg
     4561 catgagctaa cgcaaccact tttttcgtaa tctgtacatt gtcgtcgaat gatttcaaag
     4621 acgcatcgat catcacggaa gaatagccgt ttcgaatcgc ccgtaagaca tcttctatcg
     4681 taccgccatg atccaagtgg ataacgatcg gcagtttact tgtataagca taggctctta
     4741 ctgccgcaac aaactcatca cttaaatagg cgatttcatc aggatgaatt tctaagatta
     4801 ctggtgcttc taatctctct gcttcttcga tgattgcttt catcatatca tagctgcaaa
     4861 tattgaaggc tggtactgca aattgattct ctttagccac tttcaataac tctttcattg
     4921 tatataacat tacgcttcca cctcatttgt tagattttca tttttcgtcc acttgtcttg
     4981 ttttttctct ttcactgttt ttttcaatag tccaagcata aaggctgtag tgactgtacc
     5041 tgatgcaatt gccaagacat acataggtaa attgttcata acatttggaa ttagcatcac
     5101 ccaaatccct ccatgaggtg ccattgaagt aactcctact gccatggaaa tccctgcagc
     5161 gactgctgaa cctgtcatca aactaggtat cacgcggatc gggtcagata ccgcaaacgg
     5221 tatagcacct tctgtaatat acgaagcgcc taaaatccag catgattgac ctgaagtccg
     5281 ttcttctttg gtaaatttat gtttgaataa taaagtggct aaagcaatac ctaggggagg
     5341 tgtcattcct gcagccatac aagcagcaat cggtgcattt actccagctg acatcaaacc
     5401 gatcgagaaa gtcgaagtcg atttattgat cggcccaccc atatcagctg ccatcatgat
     5461 cccgataagt aatccgaaca atagcccgga tgtatttccc aagctattca gccaatttgt
     5521 caaagcatcc aacacccatg tgatcggttg ttcaatcaca aataccatca aaagcccaat
     5581 aattaagatc gataaaaagg ggacgacaat cagctgataa atagcagaaa attctttttt
     5641 tacgctaatt ttatttgcca gaaaatcaca gatataccct gctagcacgc caccaatcat
     5701 tccgccaaga aaacctgaac cacctgtaga ggccagtaat ccggcaacaa ttcctggggc
     5761 aaaacctgct tttccagcaa tcgatgtagc aatccccgcg cctaacgcag gaaccatcat
     5821 ggcaaacgct gtatctcctc cgatccttga aaatgcagct gctaacacat tatagtctgc
     5881 tgagttggga tcagccgctg taatcccaaa ggcaaaactc agtgcgatga tgattccgcc
     5941 tgcgatcaca aatggaagca tgtaattcac accattcatg aaatgattat agattgtcgg
     6001 ctttttctca tttgcagctg gttctttttc attagacaca acgaatacgc caatatctct
     6061 caatgcttcg tctattacta cttgcggttc tttgattgct cggcttgtag agacacgctt
     6121 tacttttttc ccggcaaatc ggtccaattc gatttcttta tctactgcaa gaatgattgc
     6181 atctgcctct ttgatttctt gttcagataa ctgattttct gtttttacac cttgtgtttc
     6241 gaattttgct tgatggttta gatttcttgc tgttacttct aatttttctg ccgacatata
     6301 agtatgagca attccagtcg ggcaagcagt tacaccaacg attttcatga ttattttcct
     6361 cctttttctt cactatcgat actcggacaa agactctact attttcattc ttgactaatc
     6421 tgtctgatga tatctgctgg cgtagtggct tcttttattc cctttctaaa atctgcattc
     6481 atcaatcgtc tggagagtgt gctgagaatc tctagatgca tatctgctga tttttcagga
     6541 actacgatgg caaaaatata ttcaattggc atttcatcta tcccgctata gtccgttaat
     6601 ttggctgtat gaaaaaaaca cagtgctggt tttgtcacat atttgctttt tgcatgggga
     6661 atcccaaatc catcttctat tcctgtggag atctcctcct ctcgtttgaa tagtgcctta
     6721 acgacttttt gaacatttct taccttattt gtttttttca acacatcccc tattcgctca
     6781 aataatgatc cttttgaatc tatcgatgga tcgaaaacaa tcagttcttc tgcgatcaat
     6841 tgatcaaacc tcataaaaaa cctctccccc acaaaaaatg ttaacgtttt cttggaactg
     6901 aggtcagtat agcatcgcta gctgtaccgt tcaatattat gaatgaacag aggaaaaaga
     6961 cccatcatcc gcgtgtataa gtcattaaaa tgaacgatct agctcacaaa caatcgcaag
     7021 tctataacaa aacaaaaagc cttccttgaa aggaaagctt agcttctaat tttaggtaaa
     7081 tggtttttta cttcgaaata acccctcttc atttcgttaa tataaggtgt ttttatcaag
     7141 tggataatta aaactatata ctagtttttc attgttttgt gcattatgat cctacgactt
     7201 tttcagcatc aaacgaagtt ttaaattggc aaaaaacata aaaaaacaga ctatccctat
     7261 cagatagtct gctaaacgtt gagttatcag cgtttttatt attccgcagc gtttgcgtct
     7321 ttgagaccgt attttttgtt gaaacggtcc acacgtccgt ctgcttgtgt gaatttttga
     7381 cgtccagtgt agaatggatg tgagtcagaa gttacttcga cacggatgac tgggtatgta
     7441 ttaccgtctt cccattcaac tgtttcttgt gaagtttttg ttgaacctga caagaatttg
     7501 aaaccagttg ttgaatccat gaatactact gggtggtaat ctggatggat gttttctttc
     7561 atgatttttt cgctcctttg ccctgctcca tttgctgaac cagagttgat ttgtcgattt
     7621 acaacattaa catcttaaca tgtttcagtc aaggttgcaa ttatcttttc agatttctgg
     7681 tcggattttt tttaccaaag gagacatctt gaaattcttt gaaaaattgc tgattgtttt
     7741 ttgttttacg caaaaagcgg atcagttgtt ccgtatattc taatgaatct ccagtcatat
     7801 tttttcgaag tttccaaatt tcttctaatt cttcacttgg catcaataat tcttcttttc
     7861 gggtacctga ttttttgata tcgatggctg ggaagatccg acgttcagat aattcacgtg
     7921 aaagttgtaa ttctagattt ccagttcctt tgaattcttc atagatcacg tcatccatac
     7981 ggcttcctgt atcgactaaa gcggtcgcta aaatcgtcaa actgccacct tcttcgatgt
     8041 ttcttgctgc cccgaaaaat ttcttaggtt tatacaatgc ggctgggtca atcccgccac
     8101 tcaatgtacg tccgcttggt ggaacgacta agttataggc ccgagcaagt ctagtaatac
     8161 tatccatcaa gataacgaca tctcgtttat cttcgactaa acgcattgcg cgttccaata
     8221 ctaattcaga aactcgagta tggttttgcg gctgcagatc aaatgtagaa gataccacgt
     8281 cgcctttcac gcttcgttct agatccgtta cttcttccgg tcgttcatca atcagtagaa
     8341 cgattaattc tacttccgga tagttttcgg tgataccatt tgcgatttct tttaatacag
     8401 aagttttccc tgcttttggc ggtgcgacga tcaatccacg ctgaccaaaa ccgattggag
     8461 aaaagatatc gatcattcgg gtagatagtc tttgcggtgt tgtttccagt tgaagctgtc
     8521 tttgcggata aagaggtgtt aaggctggaa aatgcggaca ttcttttgct tcttccggat
     8581 cttttccatt aacgctttcc acgtgcatca aaccataata acgttccgat tctttaggtg
     8641 gacgggcttt tcctgccact ttgtccccat ttctcaaact gaatcgtctg atttgtgagg
     8701 cagaaatata gatatcttct gcacttggtc cataattgat tggccgtaaa aaaccgtaac
     8761 catcttgacc aacgatatcc aatattcctt ccatataata aaatccttgt ttctctgctt
     8821 gtgcacgaat cacagctaga gataattcct ttttattcat cttactgtaa taaggaattt
     8881 taaattcttt ggcataacta taaacttctt ttaacgtttt gttctctagt tctgccattg
     8941 tcaaataatc gctcattcag ctcccacttc ctctatcatt tcaattgatg cccctaaggc
     9001 tgttagtttc tcgataatat gatcataacc gcgtaagata taatctacat tgtaaatcgt
     9061 cgttgtccct tctgccaata gtccagcaat cactaaacaa gcacctgccc gaagatcaga
     9121 agccacgact tctgtgccgt gaagtttatt tggaccattc acgatgatca tatttccttc
     9181 cacggtaata tcagcgccca ttcgtgctag ttccggcaca tggttcacac gtttcgcata
     9241 gatcgtatcg atgatttctg aagtgcctgt agtcatcaat agtaacgcag taagtggctg
     9301 ctgcagatct gtggcaaatc ctggataagg ataggtcatg atgtttgcgg gttttaattc
     9361 tttcgagggg taaacttcga tttcatcttc gctgattttc atatggacac caatttcctg
     9421 caattttgct ataaagcttt ccaaatgttc aaagatcaca tttttcactt tgataccatt
     9481 tcctactgca gctgctaatg ctagataggt tcctgcttcg atgcggtcag gaatcatgaa
     9541 atgccgacag ccatgtaatg tttcgactcc ttcgatacgg atcacatctg tacccgctcc
     9601 tcgaactttt gcccccatat tattcagtaa agttgccaca tctatgattt ccggttctcg
     9661 tgcagcgttt tcaattactg tctgtccttt tgcttttaca gcagctaaca tcacattgat
     9721 cgttgctccg actgaaacca cgtccatgaa aatccgattg ccacgcaatc ctttattttc
     9781 tgtccgtaaa tacatggcac catgttcatt tgtcacttca gcacctagag cttcaaatcc
     9841 tttgacgtgt aggtcaatcg gtctagggcc taaataacag ccacctggca ggcctactac
     9901 cgcttcgcca aatttaccaa gcagtgttcc cataaaataa taagaagcac gcaaactatt
     9961 gatttttccg tgcggcatcg gaatcgatac gacttctcga ggatcgattt ctagtatgtt
    10021 gttggaaaaa tggacagttg cgcccatgat ctctaaaatt tcaattaatg aatgaacatc
    10081 ttgtatgtcc ggcacacctt ccaagatcac tggggaatca gctaagattg cagctggaat
    10141 caatgcgact gcactatttt ttgcaccgct gatcgttact tcaccagcta aaggtcggtt
    10201 tcctttgatt acgatttttt tcatctgttt ccaccttgtt tattaatctt ttttcatgga
    10261 tatgcttgta ttttatcata gataaaaaaa agaaacgagc atggtctgcc gttttaagta
    10321 aaaaagaatg gagagcgact tcaaaagtcg ttctccattc ctatatatcg tatctgttat
    10381 gtggatgctt acgctttgtt agctgaaccg aaccagtcga tatgagattt agcatcttca
    10441 acgattgctg ctttacctgg agctaataat ttacgagggt cgaatccttt accttcttta
    10501 tctttaccag cttcgatata tttacgagta gctgcagcaa atgataattg gaattcagtg
    10561 ttaacattta cttttgagat acccattgag atagcttttt cgatttgatc ttgaggaata
    10621 cctgatccgc catgtaatac taaaggcatg tctggaccta cagcatcagc aattgcttgt
    10681 aagtggtcaa aagctaatcc tttccagttt tctggatatt gaccatggat gttaccgata
    10741 ccgcaagcta agtagtcgat acctgtagca accatttgtt tgcattcttc aatatcagct
    10801 aattcgccgt taccgatgat tccgtcttct tctccgccga ttgaaccaac ttcacattca
    10861 actgagatac cttttgcatg tgctttttca acaacgtctt tagctaattt caagttttct
    10921 tcaaatggta gatgtgaacc atcgaacatt actgaagtat agcctaattc gatacattcc
    10981 aaagcagctt cgtaatcacc atggtctaag tggatagcaa ctggaacagt gatgttcatt
    11041 gcttcaacta aatcagtgat taaatcttta cagattttgt aaccacccat gtatttagct
    11101 gcacccattg atgtttggat aagtactggt gcttgttttg cttcagccgc ttcaaggatt
    11161 gcttgagtcc attccaagtt gtttgtgttg aatccgccga cagcgtaacc gcctttacga
    11221 gcagctttta aaaattcagc tcctgatact actggcataa taaaattcct cctaagtttt
    11281 gtaaaactaa atttgttaag acaattctta accaacgacc ctattttacc aaacatttcg
    11341 gtagttttct actaaaatca atgaaaatga aaattttcaa gaagtttttt gcaaaaaata
    11401 aaatatccaa tagcgcttta atcgaaaaaa gtcttttgct ttctatcttt tttcctacaa
    11461 ataataacaa atcagaaaaa gagaaaagtg acaagaaaat tgcttatccc cttctcttgc
    11521 tacttttctc tttctatcaa gttcaaaaat tattttgttt cgtgatctgc taatgatgct
    11581 ccgacaaatg ctttgattaa tttttgcggg cgatttggac gagaaatcaa ttctgggtgg
    11641 aattggcatc ccacaaagaa ttttttctct ggcagctcta cgatttctac taaacggtta
    11701 tctggtgaga cacctgaaaa gactagccct tgttcttcaa acaattgacg atatttgtta
    11761 ttgaactcat agcggtgacg gtgacgttct tgcacaacat cggctccatc atatgcttca
    11821 gccgtcttcg ttcctttttt gattttacat gggtataatc caaggcgtaa agttccaccc
    11881 aaattctcga tgttttcctg atcagccatt aaatcaataa tattgtttgc tgtatctgga
    11941 ttcgtttcag ctgatgacgc atcttctaat ccaacgacgt tacgtgcaaa ttcgacacaa
    12001 gccatctgca taccaagaca aatacctaaa aacggaacgt cattttcgcg agcataacga
    12061 atcgcttcga ttttcccttc tactccgcga tcaccaaatc caccaggaac caagattcca
    12121 tctgcgtctt tcaaacgttc ttctacattt tctctcgtta attcttgtgc tttgatccaa
    12181 tcgatctcga tgtctgaatt ataggcgaaa ccagaatgtt tcaatgcttc aactactgag
    12241 atatatgcat ctggtaactc gacatattta ccgacaagcg caatccgtac ttttttcttc
    12301 aagttcaaga cacgttcttc taattctttc cattctgtca tgtctgcttc tggtgtatct
    12361 agttttaaat ggtcgcaaac aatttgatcc attccttgtg cttgtaaaac taatgggatg
    12421 gagtaaagtg tatctacatc tggcgactcg ataactgctt ctggttcgac atcacaaaat
    12481 tgtgccaatt tgtttttcac gctttgagaa acaggttgct ctgtacgtac caccaaaata
    12541 ttcggttgga tacctaaacc acgtagctct tttacactgt gctgcgttgg tttagttttc
    12601 atttctccag cagctttcaa gtaaggaatc aatgttgtat ggatatacat aacattatct
    12661 gtaccgacat ctgctttcat ttgacgaagt gcttcaagga atggcaaaga ttctatatcc
    12721 cctactgttc cccctacttc tgtaatgatc acatctgaat cagtcatttt agctgctcgc
    12781 atgatttttt ctttgatctc atttgtaata tgaggaataa cttgaacagt tgctcccaag
    12841 tattcgccct tacgttcttt acgcaaaact tcagaataga tttttcctgt cgtcacgttg
    12901 gagtatttgt tcaaattgat gtcaataaaa cgttcgtagt ggcccagatc caaatctgtt
    12961 tcagcaccat catctgttac gaatacttct ccgtgctgat atgggctcat cgtccccgga
    13021 tccacgttga tgtatggatc aaatttttgg atagttactt ttaagccgcg gtttttcaat
    13081 aagcgtccta aagaagctgc aacaatccct tttccgattg atgaaacgac accgccggtc
    13141 acaaaaatat attttgtcat aagttcaaaa actccttttt aatttatgtg caggaaaagt
    13201 ctcagacaat aaacaaagct ccctattctt caataagaat agggagccga aatttctgta
    13261 aacttctgac cctctaaaag ggtgcccaag gtgtataata caagctgact aagggaaagt
    13321 caagtactca aatgaatact atccgcttac aattttttct ttttcgatat cctttgagaa
    13381 attatttctt aagtccttta tgagtgcaaa aaaaatttaa cccacataat aagcttgata
    13441 taaacatagc taacgttaaa cctaagcaaa tcagaaaatc gttactgaaa aattttaagt
    13501 gaaataatag ataaaaaaat aaaacaaaca ttatgataga taatatccca cctaacaaag
    13561 aactactttt tcctacttgc agcaacagtt tatatagctt atcgtagaaa caatcttttt
    13621 ctttcaccct tatttacctc ctttacgtaa aaaacgtagt ccacagttct cggatttttg
    13681 gcatatcttt tctattaaat aatatcaaaa acaaaacaat tccactaatt ttttcaaaga
    13741 tgaccttttt ttcaacaaaa gtgccttgta gggattcatt gaaggtaacg aatcacataa
    13801 gtaaaaaata aggagatgcg acaaaagtcg tgcctcctta tttctaaata aacggtgttc
    13861 tcctgccaac tcctcggtca caagtcacaa tatccatcaa acatgagaag ctgtttttga
    13921 aattatttct tgtgattcgg agttaaatgg taggctcaca acctctggat aaacggtatt
    13981 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca
    14041 caaaattttt cttaatactc gaaactaaac gtctttttca aaacctcttg gtaaacggtg
    14101 ttcaaaagct tgcatctgcg gtaataagtt cagccaattc aaaaacaaga aaagctgttt
    14161 ttgcttggct attcttatta cttgttgcag ggcagctttt tcacaacctc tccactaaac
    14221 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
    14281 ttttccgtta gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac
    14341 ggtattcctg ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg
    14401 tctgaatgcc gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc
    14461 taatgcgtca atcattacat tatccttacg caattctttg atagcattat ggatagttga
    14521 aggtaagtcc tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg
    14581 gtcaacagct gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa
    14641 tactgccatt gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt
    14701 ggataaaccg cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc
    14761 gacataaaca ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt
    14821 gcaaacggct gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt
    14881 ttcacttagt tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa
    14941 agacatattg caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt
    15001 cgcgtgaagt ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt
    15061 atcacaagca tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac
    15121 ttcatgatgt gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg
    15181 gcggcaattt tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa
    15241 ggtcgttgtg atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc
    15301 taaattgaaa gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc
    15361 gcgaggatcg ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc
    15421 aacttttcca tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa
    15481 atacatatcg ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat
    15541 tttattttct aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc
    15601 taagatgtct gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt
    15661 gatttcttca cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat
    15721 tcagtcaaag aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc
    15781 accttgcgcc aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt
    15841 cttgtttttt gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc
    15901 catattcaat ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag
    15961 cgaatacata cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata
    16021 atagcgaatt tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac
    16081 ggacatagat cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt
    16141 ggctaaagtg taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat
    16201 gagatctcta ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg
    16261 attttcgtac cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt
    16321 tatt
//
LOCUS       DACDYM010000059        16092 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDYM010000059 DACDYM010000000
VERSION     DACDYM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.059"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..349
                     /locus_tag="IWM59_001940"
     CDS             <1..349
                     /locus_tag="IWM59_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9979968.1"
                     /translation="TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDT
                     RHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIG
                     LRITFMSYKKSKR"
     gene            complement(504..1490)
                     /locus_tag="IWM59_001941"
     CDS             complement(504..1490)
                     /locus_tag="IWM59_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9979969.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1513..2079)
                     /locus_tag="IWM59_001942"
     CDS             complement(1513..2079)
                     /locus_tag="IWM59_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9979970.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2093..2509)
                     /locus_tag="IWM59_001943"
     CDS             complement(2093..2509)
                     /locus_tag="IWM59_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9979971.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2966..4900)
                     /locus_tag="IWM59_001944"
     CDS             complement(2966..4900)
                     /locus_tag="IWM59_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9979972.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5202..6137
                     /locus_tag="IWM59_001945"
     CDS             5202..6137
                     /locus_tag="IWM59_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9979973.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6227..7330
                     /locus_tag="IWM59_001946"
     CDS             6227..7330
                     /locus_tag="IWM59_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9979974.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7343..8296
                     /gene="menA"
                     /locus_tag="IWM59_001947"
     CDS             7343..8296
                     /gene="menA"
                     /locus_tag="IWM59_001947"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9979975.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9110..10456)
                     /gene="gor"
                     /locus_tag="IWM59_001948"
     CDS             complement(9110..10456)
                     /gene="gor"
                     /locus_tag="IWM59_001948"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9979976.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10590..11831)
                     /locus_tag="IWM59_001949"
     CDS             complement(10590..11831)
                     /locus_tag="IWM59_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9979977.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11849..12772)
                     /locus_tag="IWM59_001950"
     CDS             complement(11849..12772)
                     /locus_tag="IWM59_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9979978.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(12993..15485)
                     /locus_tag="IWM59_001951"
     CDS             complement(12993..15485)
                     /locus_tag="IWM59_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9979979.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15492..15959)
                     /locus_tag="IWM59_001952"
     CDS             complement(15492..15959)
                     /locus_tag="IWM59_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9979980.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4723 a         3356 c         2863 g         5150 t
ORIGIN      
        1 taccactaca agtgatagca cggacacgtc agcttcaagt gacagtacag atacatcctc
       61 taccacaagt gatagctcag attcatcaac ttcaagcgat agtacagaca cgtcgtctac
      121 cacaagcgat acacgtcatg caagtgtctc cttctcaaaa acaagttcgg ataacaggac
      181 gaactttaac ttaactaata aaacaagcaa tgacaatgga aacaaatcaa aaagagcact
      241 tcctaaaact ggaagtcaat cgaataattg gataacactc gcaggcgtta tattacttgt
      301 aatcggttta agaatcactt ttatgagtta taagaaatcg aaaaggtaaa aaaatattca
      361 agataaaaaa gagcaacgtc gctaacggac gttgctcttt atttttgctt tttatcataa
      421 atacaagaac tacaattcaa tttgtcaaaa aacacgtaga tttccttctg agaaatttat
      481 ttacgtatcg aacctttccc tattcagtct ttgcgatcca aaatcagttg ggtaagggaa
      541 aagagattct cttttgttcg aagagaggtg gcaggtaatt tctttatttc cttcaaagcc
      601 ttttctgtgt aagaggccgc taacttccta gcttcttcca cgcctcctgt atgttcaacg
      661 attttgcgta cggcgtcgac atcttcttct gtcattgccg ctttcttctc taaataaggt
      721 agtagctttg gtttgttttt ttgcaaagag tagatcaacg gtaaagaata gaccccttga
      781 cgcatgtctt ctagtacagg tttgccgatt tcctggctct cttttgtata atctaaaata
      841 tcatcgataa tctgaaaggc catcccgata gaaagtccga tatctcctgc tttttttgcg
      901 aagcgctcgc ttgttccact ttcaaaagcg ccaacagaac aacttaattg gaacagttca
      961 gctgtcttac ctgaaatatt ttccaaatat tcttttacag aaagttcata attatagcga
     1021 tcatccatct gccctaattc gccattcaat actttttcca tactgcgaga attcaattgg
     1081 agacttttca aggaagtgga gtaatcagac aatagtttaa aacagcagac aaataaatag
     1141 tctcctgcat agacagaaac gacattacca aactgactac ggatattagg ctgaccacgt
     1201 cgcagatccg cttcatcaac gatatcatca tggatcagtg tggccgtatg gagcatttcg
     1261 atagaagctg ccaaagcgat ggctttgtct cgatcttgct ctggtccaaa ttgtgagaac
     1321 aatagctgat aggcaggacg caacagcttt ccgccggcat ggatcgtttc tttgatggct
     1381 tgttcgacag gtttgttttt caggcggaca gtttcttcca tccgttttag tgtttctgtc
     1441 aattctttac ttaaagcagg atagttttcc cacattggat gtagcttcat aaaaaaactc
     1501 cttgtgtgac gattattttt tggtaaacat tgggctccat ttttgtttgg ttccctgctc
     1561 ataatctttt tggaatcgtt gaagtgtctg cacatgtcgt aaaagataat tcgctgcgat
     1621 cccgattgca atccctgata aaatccctaa aactgaaagg actggcaagt acaacatgac
     1681 tgtccatgat tgtgcaatga aagaagccat caaaagctgt ccgacattat gcatgaaacc
     1741 gccggccgca ctaattccga tgatactgac acgttttggt cctaattgct tgaccaataa
     1801 cattccaaaa taactaagca aagcaccact catactataa aggaatgtgg aaatcgttcc
     1861 gcctagcaaa gtagtcagga atagtcgtag ccaaagtagt aagaagctgt cttgtttttt
     1921 catggtaaaa atggcaacga tcgtgattag attggctaag cctagttttg caccaggcgc
     1981 aaaagaaaaa ggataaggga tcatattttc caataaacca atgatcactc cttgtgcaac
     2041 aagtagcgaa atataaatca ttttttgtaa tttactcatg gtgtgtgaag gtctaataaa
     2101 tcagactgtc ttcgtcacct ccatctgttg aacggacttc gatcactaat ttatgtggca
     2161 aacagacgat ggtttcgcca tttttcgttg cccagcctcg acgtacgcag atctggtcat
     2221 cgcaatctgc ttcttttatc cgtactcgat cgccatctat ctcgatcaag ttataatctc
     2281 cgtgttcatc tttgtaagta tatgtgttgc tctcttctcc agccatcaaa gggaatgttt
     2341 tgatttctgt gccatccaca cgcagtaccg cttctttttc tgctgttgcg gtgttttgct
     2401 gaagaccaaa aatcatgact ggtaaaaaag aaagaacagt caaaagcaaa ataataaaaa
     2461 catcccaagg tttgagcaga cttttttcag caaattcttt aaggctcatt tttgttctcc
     2521 tttttccaga ttaactcacc caattttagc ataaaaacaa gaggttgtga cacctgtgtc
     2581 ttgctttgag taataagaac gaagaataga aaacagcttt ccatgttttc taattctttg
     2641 ggcttattac cgagaagcag caggtggaac accgtttata acaagaggtt gtgacagaag
     2701 tgggcaactt caagaaataa gaaggaattt acgaaaatta cttttcaagt ttttgtgaaa
     2761 tttcggctta tttttcgaag aagctacttc tggaacaccg tttataacaa gaggttgtaa
     2821 cagaggttgt gacacctgtg tcttgctcat ggaggcttat ctcgggcatc ttattccttt
     2881 aattttacat aaaaaggagg ctgtgacggt ttgtctcagc ccctttctat tgaaatcttg
     2941 cattcaaata acgttcttta tctatttatt tcttgccgta ctgggaactt ggtctaccgt
     3001 accaccaatt acctagtgta cgttgtagcc atggaattac ccagcgatcc aacccaatcg
     3061 cacgtccgga accgttcatc aatgcgaaag caacaaaaat gaaccaaatg tttacccagt
     3121 aaaacatgcc tgataagcag aacgtgatca ccaatacaat cgttgtcgca ctgcatagcc
     3181 atgtgaataa tccagcaatc aatgctaatg ccaaggcaat ttcaaagaat accatgaatt
     3241 tttggaagaa cattgctaca tcattgtttg gcatgatcac ttgcatgatc tttgtgaacc
     3301 agtctggtga cttctcaatc acagccattg gttcttcacc gtatgcataa ctcaaaccaa
     3361 aatgtgcagc ttgagtcgct gcttctccgg cacttgcccc tgctccgctg gcagctgtgg
     3421 ttgcatcacc agcagcagaa gcaccactgg caacatctgt tacattgtat tcccatgtcc
     3481 aagggaacac actgtttcca gtgaaccatg atccttcacc aaaccaagta cttggtttga
     3541 acacttcacc attcccaatg atttttttgg aagcttcaac gaaccaaact aaaccataga
     3601 aaatacgaag tggcacactc cataagacat tgccgtaacg agaagaatgt cctcgagtca
     3661 cgttgcgatc atctttgata tggaaaaact catgcattaa atattggaac atataataac
     3721 cagaacgaat atcaaagaag tatttcaaat tcacgatatg tttcattacg atagctaaaa
     3781 atccgcttaa atggatctta tcaaataggt tagctacccc ccatttggca ccaatagaaa
     3841 ccataaatcc ttgatagttt cctttaaatt tatgcttttc tccgcctttg atcgaagcca
     3901 cgatattcgc tgcggctgtg tgtcctgttt gttcagcagc ttgaacgatc tgcggtgtcg
     3961 gtgttcctgg gaattcttca taaaagacta aatcaccaat gatatagatg tttttatctt
     4021 cgtagccttt tgcctgcata tattcattgg cgatcaaccg ctgtccgcgt gctgattcta
     4081 atccaaagtc agcagcgtca gaagttcctt tgacaccagc tgtccaaatc agtgtgtgag
     4141 tcggaatgtt gcgtccgtct ttcaatttga tatgatcagc agccacttct acgattggtg
     4201 cattcaataa taattccacg ttgtgcttac gcaaatagcg ttcagctttt gcagcatcat
     4261 ttcgatccaa catattcaag atcgtcggca ttgcctctac gaccatcagt gtgatttcgt
     4321 ttggatctaa tttataatct ttggctaggc gatttttcca gtcgatcagt tccccaatca
     4381 tttcaatacc ggtaaatcca gaaccacaga caacgaaagt cagcatggct ttacgcagtt
     4441 catcatctgg ttccaatgca gcttttgcta ctgtcttctc aatatgttcc cgaatcctta
     4501 cagaatcttc aaatgaccaa agggtgaatc cgtgtgcttt gacaccaggt gtaccaaagt
     4561 cattcggctc tccgcccatt ccaaggatca gctgatcaaa aggataggaa cctaatttgg
     4621 tcttgacgat ttttcgttct ttatcgatac cgattacagt gtctgtgaca agttgtacat
     4681 tcttttgacg agcaaacaaa tgctgcagat cgtattggat tgcttctggt tctacacgtc
     4741 cgccagctac ttcatgcaac tcggtcatca tagtatggta cgaatgacga tcgatcagag
     4801 taattgctac atcttctttt ttcagtttct ttgccaagta ttttgtagct gaaacaccgg
     4861 catatcctgc tcctacaacg acaatatttt gctttgccat tgatcatccg ctccttatag
     4921 actatctata taatgaatat atgataattc ttttagtttg atacttatta cacaatctcc
     4981 atatattata tagatgttct gttcttttgt ctaactcctt ttggattttt ttcaaatttt
     5041 aaaataaagc gttttttcac gaaatttgct gaaagaaaac gtttgaaata ttacatgggt
     5101 tatttatcaa aaatagttga cttataggct aaaaagatta gaatgtataa tgaatcacaa
     5161 agaaaaattg tggactagaa atggaatggt ggggtattaa aatgaagaaa aaacgttttg
     5221 taactggatt tgccgtatta gcattttcag ctctagtgtt aggcgcttgc ggcgcagatg
     5281 acaacggaag ctcaaactct tcttctgaat catctacagc acaatcttcg actgcaaaaa
     5341 gcacaacaga atcatctgca aaagttgtag ctggcggaga tttgcaagac ggcacatata
     5401 aattagaaga aaaaaattat tcaaatggtt accgtgcagt ctttgaaatg gttgtaaaag
     5461 acggcaaaat cactgaatca aaatatgata acgtcaatga aaacggcgaa tcaaaaacaa
     5521 aagatgcaga atacaacaaa aacatggaag caaaatctgg cacaaaccca gaaaaataca
     5581 ttcctgagtt gaatgaccaa ttcttaaaag cacaaagcgc aagcggtgtg gaagtcgtaa
     5641 ctggagctac tcactcatct gaaagcttcc aaaactacgc acaacaattg atccaagctg
     5701 ctcaagctgg taacacagat acaatcgaaa tcgacaatgg tgctgatttg aaagacggca
     5761 cttacaagct tgaagaaaag aactactcta acggctaccg tgtgcaattc gaaatgacag
     5821 ttgctggcgg caaagtaact gaatcaaact ttgattatat cgacaaagac ggtaaatcaa
     5881 aacaagatga tacagaatat aatgaaaata tgaaagcaaa aagcggtaca gaaccaaaaa
     5941 cttacatccc tactctgaac gacgaattcg tcaaagcaat gggcgaagaa gatggctcac
     6001 ctgctaatgt agaagttgtt acaggtgcta cacacagctc acattcattc atcatgtacg
     6061 cacaacaatt agtgaatgca gctgaaaaag gcgacactca aacaatcgaa gtagataata
     6121 tcgtaacaga aaaataatca cataatcata ctaataaaag ttggagggtg ggacgatagc
     6181 cagattaggc agtcgtctca cctttcaaaa tgaagagagg aaaaagatgc ggaaaaaact
     6241 actattttcc ttaatcacac tgggcatctc ttttatggtg ctaactggct gcagcaactc
     6301 agcaaataaa acaaccactg aaagttcagc agctaccaag atattgaaag atccatattc
     6361 tgatgaacag ttcttgttag ggacttacgt tcgtatccgt gtgtatgatg aaggcaaaga
     6421 atcagcattg aaacctgcct tcgatcgagt aaaagaactt ggtgataaaa tcacgatcaa
     6481 tcaaaaaggt tctgagatcg atgaagtgaa tgagcaagct gggatcaaac cagtaaaagt
     6541 ttctgatgac gtctacacgt tagtcaagcg ggcctacgaa tatagccaag attctcaagg
     6601 cggattcgat atggcgattg gtgcaatcac acaattatgg cgaatcggtt ttgatgacgc
     6661 cagaaaacct tcccaagaag aaatcgatca ggcattgaaa ttagtggatt atcataagat
     6721 cgaactaaat gacaaagaaa aaactgttta tctaaaagaa aaaggaatga tcatcgattt
     6781 aggtgcaatc gccaaaggtt acattacgga cgaagtagtg aaagtcttga aaaaacaagg
     6841 agtgaccact gccatcgttg acttgggcgg taatgtctat gtcttaggcc acagcccaag
     6901 aggcgaaaac caagactgga cagtcggcat ccaagatccg aacatggctc gcggatctgt
     6961 tttaggttct attaaagaac ggaacaaaac attagttacc tctggaattt acgagcgtta
     7021 tttagaagta gatggcaaaa aataccatca tttgtttgat ccaaaaacgg gttatccgtt
     7081 cgacaatgat atcgcaagtg tcacgatcat aacagataaa tcaattgatg gtgacgggtt
     7141 gtctacagca gtcttttcaa tgggtgtgaa aaaaggactg gaatacgtcg aaagcgaatt
     7201 gaataacggg acggaagcga tcttcgtgac aaaagacgat aaagtatatg tgaccgatcc
     7261 aatcaaagac accttcaaat tatcggagga ctcccattac acgatgggtg accgttctga
     7321 actaaaataa ggagtacgaa ccatgacact aaaaatcttt ttagaagtag ttgaaatacg
     7381 aacaaaggtg gctagtgtat ttccttttgc ggtaggggta ttattctcta tcgctttttt
     7441 tcatgagata cattggctaa acactttttt attttttatt ggaatgctag tttttgattt
     7501 agcgactacc gctatcaata attacatgga ttatgaaaag gctcattctg aagtatataa
     7561 gtatgaagaa aatgtcattg gccgcagaaa tctatctcct gtattgattc gaaacatgat
     7621 tttagctatg attgcatttg tttttgttat cggcgttatt ctaacgatca atacgggttg
     7681 gcttttactt ttgatgggat ttatctgttg ttttattgga gtattctata cattcggacc
     7741 gattccttta tctcgaatgc ctttagggga aattttcagt ggggtgacta tgggccttgg
     7801 aatttttgcc atggtcgttt atgtcaatac attcggttca gatgtctatg atctgacagt
     7861 caacttttca tctggacaat ttgatttggt aggaaatctt tgggctatag cagcaatcgt
     7921 attagcttcc ttacccttgg tgtttacgat tgccaatatc atgttggcta ataatttgcg
     7981 ggatcttgac cgtgatattg aaaatcatcg ctatacacta gttttttata tcggacgtcc
     8041 tgcgggaaca gtcttattcc agctattgat gtatgcttgc tatttcgtga ttttaatggg
     8101 gttgttgagc ggtatctatg aatggccgat cttggtcacc ttcttgacct tgccaattgt
     8161 gaagaagcat ttggatcaat tcaagcaaac gttaccccag cctatcagtt tcagctattc
     8221 catcaaaaat atggtcttat tcaatgccag ttatgtattg gggctattac taacaatcat
     8281 tatgcaagcc atttaacagc atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata
     8341 agaacagcca aataaaaata gctccttata tttttcgttt ggttgggctt attaccgcag
     8401 atgcaagctt ttgaacaccg tttatcagga ggttgtgaaa aagtcgctta gtttcgagta
     8461 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg
     8521 agaaactggc ttttgaacac cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt
     8581 attagagaaa caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg
     8641 aaggacagct gattgaacac tatttgttcg gaaaaagtga ctaactatga cacaattttt
     8701 ttcacagtcc cttttttcac ttgatttgat tcgtaccagc gaacttgacg agaactttct
     8761 tggtttggta actatatggt attcttttaa catcctcgtt tgcttcttct aatttattta
     8821 tacaaagtca cactttttta aacatctgtt cttcgataca tttctatttc ttttaaacat
     8881 cgataccaat attttgaact taagagtgca taccaaatgg attcatttac tcttcgtcag
     8941 ttacgaagat aatcaaaatc tccaacttcc ctacttattt cgttttgaga attatcgaag
     9001 caatcttaca taaataacta aaaaacaaaa aaccagaaaa tcagtatact gattttctgg
     9061 ttttacatgg tttgttcaat aaaaagctgt tggaactgtt aatattaaat tatcgcatcg
     9121 ttacgaattc ttccgctcct gttggatgga tggctactgt attgtcaaag tcttctttag
     9181 ttgcacccat cttgatagcc acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa
     9241 tagcatgtaa gccgacgatt ctttcttctt ttccgacaca gatcaatttc atttcgcatt
     9301 tttggcgata atcatttaat gcaaagtaca ttggggtaaa gcgtgaacga taaactttca
     9361 attcattttc gccatatttt tccaatgctt cttcttctgt caaaccaatt gttgcaattg
     9421 gtggatgcgt aaagacgact gtcggaacaa gtttatagtc aagataagaa tctttctttc
     9481 cattaaataa acgttctgat aaacgtctac cagctgcgat tgcaacagga gtcagatcta
     9541 atttaccgat cacatcacca acagcaaaaa tatgttcatc cgttgtattt tggtatttat
     9601 ctactttgat aaaccctttt ttgtccaatt ccacatttgt attttccaga cctaattttt
     9661 cggtattcgg tacacgtcca ccggcaaaaa gtacacaatc accagttaag gtttcaccat
     9721 tttcaaatag caccgtatat tcgtcattgt ttttagtaat ttctttagca atatgatgtg
     9781 cgtaagtttt tattccagct tcttggtaca tttggatcaa attgtccgaa agcattttat
     9841 caaacgttct aagtggtcgt tcttttcgat atgcccacat gacttcacta cctaaaccac
     9901 gaaatacacc tgaaagttca gccgcaatgt atccagcacc taaaacgatt gctctcttag
     9961 gtaactctgt taattcaaaa aatccatttg aatcaattgc gtattctcca ccaggaatgg
    10021 tcattttact tgcacggcct ccagtagcga tcaagatttt aggagcacga taggtttcac
    10081 cgtttacttc gatcgtttga ctatcgataa aagtagcgta accttttata gcctcgacat
    10141 gattgctatc taatcctctt tgataagcac cgtgtagaaa atcaatatat ttttctcgtc
    10201 tttctactag accggcgaaa tcaacatttt ttaaatctgc ttggataccg taacttgagg
    10261 catctcgttc aattgtttcc aaaatcgtac ttgcttgcca cataactttt ttaggtacac
    10321 aaccgacgtt gacacatgtt ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc
    10381 catgcatccc tgctctgttt gctgaagcaa taccgccaga gccgccacca attacgatgt
    10441 aatcatatat ttgcattttg ttcctcctta ttgaagagat caatcaaaac ctttatttaa
    10501 cgatcctttt tctacagaaa acaagtgtaa cacttttctt gaattttgta tgcgaagatg
    10561 cttaaaacag ggatatatca gctttttcct tatttacgtt ttcggatcat atctccaggt
    10621 ttcaccggct gtgcgactgg catagttaaa atcatcattg gatttggtgc tcggtcaatg
    10681 gattcgcctt cttcattatg cataacttca acggtttgac tgaaatgact gaatcctgga
    10741 ccataaaatt cgatttcatc tcctacagaa aacaagtttc tctgacgtat cgtagcaatc
    10801 tgtgttgcct catcataaga taatacttca ccgacaaatt tatatactgg gattttacga
    10861 cgttcaccga ataattgttc gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat
    10921 tctcgttgtg ctactttcca taactcgtcg atccattctt gcttgcagac atagttttca
    10981 ggatcttcca tataactgtc tacagccgct ttatagacat ttgacaccgt agatacataa
    11041 tggatcgatt tcattcttcc ttcgatcttg aagctgtcta cgccgttttc aatcaattct
    11101 ggaatatgtt cgatcattga catatccact gcactcatcg agaattcttc ttccacttcg
    11161 cctgtatcag ttaaactgtt tcgttcttga ccaaaaggca tatcgaacaa tccatacttc
    11221 cagcgacaag attgagaaca tccgccacgg tttgcatccc gcatcgacat atggttggac
    11281 aatgtgcatc gaccagaata agagatacac atggcaccat ggataaatgc ttcgatttcg
    11341 gtatccgtat ttttacgaat ctctgccact tcctccattg aaacttcacg agctaaaacg
    11401 acacgttcca acccttcttc tttccagaat tccaatgtct caaaattagt tgctgaagct
    11461 tgagtggata aatggatcgg cagacctggg gcttctgtcg cacaaatttc gatcaaagct
    11521 ggatcagaaa caatcactgc agaaataccg acatcacgca aactgcggaa aaattcccct
    11581 gctccatctt catttccttc atgggtgacc atattagcag caacgtatac cttcgcatca
    11641 tgttcttttg caaaagcaac tccttcagcc atttcttcat aagtaaaatt acctgcacgg
    11701 cttcttaaac catatgcatt cccgccaata tatactgcat ctgctccata atggatcgcg
    11761 gtttttaact tttccagtgt tcctgctggc gctaggattt ctggtcgttt caattttctt
    11821 tttactgtca tttctttcat catactcctt atttgatagc agaaggatca atataaaaga
    11881 atcctgtatc taattcacgg ttttcaggat gcaatttact gatttcgcta gtcatttttt
    11941 ccgctttttc ttgatcccag ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc
    12001 gagtgatgtt tacgaatttt tcgcctggag taaataatcc gtccaatttc catgtacagt
    12061 aatgatgctg ataaagttca ttcaattcat tcgagagatt cagatcattg ctggcaaaaa
    12121 tatgtgtgcc atgactatct tcataaatag aataatgtgt ctcttctttt ttaggttccg
    12181 agataaaaag acctctttca cgatcttttt gttcgtcttg tttcgtatag ttatagtaat
    12241 tctgtagcag tggacgtttg gattgatgga tgcatgtagc accatacaca agggtttcta
    12301 caggaatatc gagtttttct tccatcgcct tcatctcttc aaaaggaact tcacgagcaa
    12361 gtactgctcc aactgcgcct tttttcgccc agaaattgat ttgacgtgcg ctcgttacta
    12421 atgtttcagc atcataaata tacggtatac gcaattcagg attttttttc atcgtataaa
    12481 taatacctgt atcaccaact gtgatcacat ctacgtttat ttcttttaaa aaagccaaat
    12541 attcaggtac atttttcatt ttttcaggat gaatgatccc atttacagca atattcgctt
    12601 ttttaccata cttgtgtgct aattctacca attgtcgctg ttcttctcgt gtaaatgaag
    12661 caggcagacg caatccaaat tgctcttcac cgaaatatag tgtatctgca cctgcagcaa
    12721 gcaactgttc tgcttgttca atagtttcta ctgtggttat gagttcgatc ataattttct
    12781 cctcctacaa cttcataata gtagcatagc tctaaaaact ttccaatact tcttctattt
    12841 aaagtcggat ttttgaaaat tatttcacaa aaaatcaaaa taaagttcga tatattctgt
    12901 ccaaatataa aaagagattg tgacaaaaat cttgtcacaa cctcttaatc aagtcttact
    12961 attataaaaa ttcagctaca caggccaagt aactataggg aacctactgt ttccttttta
    13021 ggtccgcgaa cattcaatgt gatcttacct tttgaagcac ctactgttac gtggtcacct
    13081 aaacgaattt ctccgccaag caaagcctcg ctcaatcgat cttcaatttc tttttgaagt
    13141 gcgcgacgga ttggacgtgc accatattca ggatcaaatc cagctttccc gatcacatcg
    13201 attgcagatg gtgtgatttt cacttgaata tcttgttctt tcaatcgttt gataatcgct
    13261 ttgctcatga tttggacgat tgtatggatt tcatctttac tcaatgaatg gaagacgaca
    13321 gtctcatcga ttcgattcaa gaattcaggt cggaaagctt tctttaactc ttctaagata
    13381 cgtttttgca tggctttgtg atctttagta acatcttgga cattgaatcc gacatttttt
    13441 tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa tcatgattgt atttctaaag
    13501 tccacttttc tacctttaga atctgttaaa tgaccatcat ccaatacttg cagaagtaaa
    13561 ttgaaaacgt ctggatgtgc tttctccact tcatctaaga gaatcactga atatggtttt
    13621 tgacgaattt tttctgtcaa ctgtccacct tcatcatacc ctacgtatcc tggaggagac
    13681 ccgattaatc ggcttgtact gtatttttcc ataaattctg acatatcaac acggatcagg
    13741 gcatcttcac ttccaaacat gacttctgaa agtgctttgg ccaattctgt ttttccgaca
    13801 ccagttggtc caaggaacat gaatgaacca attggacgat caggatcttt caatccgctt
    13861 cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct catcttgacc aacgacacgt
    13921 tcatgcagaa gtccttccag ttctaacaag cgttcgcttt cttttttctc caactgctgt
    13981 agagggacac cagtccactc cgatacaact gtagcaacat cttcttctgt tacacgatca
    14041 gaataacctt gctcctgttt tgcttcaatt gcagaaactt tttgcagctt tttacgtagt
    14101 gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt cttgtttttg gatcgctgct
    14161 tccttttctt gtactagttc atcgatttct agtttgatca cagttgattt tgttaagtga
    14221 tcggtttgat ccaaacggac tttcgctgct gattcatcga tcaaatcaat tgctttatcg
    14281 ggtagttgac gagaagtgat ataacgaaca gaaagattga ctgctgctcg tacagcttca
    14341 tctgtgatct ccactccatg gtgctcttca tatcttgtac gtaatccttg aagaatcact
    14401 tctgcttctt caggagttgg ctcatctact tgaatgcgag caaaacgtcg ctctaatgca
    14461 gaatcttttt cgatatattt ttgatattca tctagggtcg ttgctccgat cgtttgtaat
    14521 tctcctcgag ctaacgccgg tttcaagata ttcgaagcat caattgctcc ttctgctcca
    14581 cctgcaccaa ttaaggtatg caattcatca atgaataaga ttacttgacc atcattatag
    14641 atttcgtcga tgaccttttt cattcgatct tcaaattcac cgcgatattt cgtaccagca
    14701 accaaagatc ccatatcaag catcatcagg cgcttttctt gcatgtcttc tggcacttca
    14761 cctaaaatga ttttttgagc taacccttca gcaatggctg ttttgcctac accaggttca
    14821 cccaccagta cagggttatt ctttgtccgt cggctaagaa tctggataag ccgtttcact
    14881 tctctcgaac gaccaacaac tgggtccagc ttgttttctt ttgctaattt cgtcaaatca
    14941 cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt gccgtttatc ttgtacattt
    15001 acatttctac gacgattcat tccttttcca gcagaatcac cagctagccc cattttttgt
    15061 ttcagaactt gacgggcttt tgaaaggctg atacctaagt tgatcatgat tcgagtagcc
    15121 atgatacttt catcttttaa taagctcatc aaaatatgtt ctgttccaac tttttgagaa
    15181 ccagttttct tcacttcgct gctagctaaa gcaaaaactt gtttcattct tggtgaatat
    15241 ggaagaaaga ctcctttagg gtaagaacgg ataccaccat aaccaatcag atgttcaatt
    15301 tcttcataaa tttccgtttc attcaaatcc atttcacgta atacttttcc agcaattcca
    15361 ttttgctcga ccactaaggc tagtaaaaca tgttctgaac taactgattg atgtttcaac
    15421 cgttttgctt cttcttgtgc aatttctaat actgcttggg ctctttcagt aaaaagttct
    15481 tccatctaat ctcacttccc atcttcataa cttaaacgtt ccagaaatgc tcggagaatc
    15541 cgagcacgtg tatgatcttc atattcattg aaattcaatg tgttttttgc aattgcactc
    15601 aacatcaagt ttccttcttt ttttgtaatt atttggtctt cataaagttt ttgaataata
    15661 gcaaaagcat ttttctcact gattgtatca tcaaataact gatttatctg ctcaagtaat
    15721 tgttttttat ctgaaatcct tacttttgca attcgtatat aaccaccacc accacgtttg
    15781 ctttctacta gataaccacg ttggatggta aaacgggtat taatcacata gttgatttgt
    15841 gaaggaacac agttaaagag gtctgccatt tcaatccttc gaatctcgat catttcacta
    15901 ttttccagaa ttttctttat atatgattca attaaatccg aggtattctg atgtgccatc
    15961 ttattcattc cctttgacta atattgacct taattataac gaatttgata atagaaagaa
    16021 agaagaacgc tttttgttaa ataaatcctt tagaatcaac aaaaaaaaat acggaatata
    16081 ctattccgta tc
//
LOCUS       DACDYM010000060        16075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDYM010000060 DACDYM010000000
VERSION     DACDYM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.060"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="IWM59_001953"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="IWM59_001953"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="IWM59_001954"
     CDS             complement(227..697)
                     /locus_tag="IWM59_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9979981.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="IWM59_001955"
     CDS             complement(856..1314)
                     /locus_tag="IWM59_001955"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9979982.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="IWM59_001956"
     CDS             complement(1485..2630)
                     /locus_tag="IWM59_001956"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9979983.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="IWM59_001957"
     CDS             complement(2643..3560)
                     /locus_tag="IWM59_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9979984.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="IWM59_001958"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="IWM59_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9979985.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            5037..6296
                     /gene="lysA"
                     /locus_tag="IWM59_001959"
     CDS             5037..6296
                     /gene="lysA"
                     /locus_tag="IWM59_001959"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9979986.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6283..7083
                     /gene="dapF"
                     /locus_tag="IWM59_001960"
     CDS             6283..7083
                     /gene="dapF"
                     /locus_tag="IWM59_001960"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9979987.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7086..7967
                     /locus_tag="IWM59_001961"
     CDS             7086..7967
                     /locus_tag="IWM59_001961"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9979988.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7964..8680
                     /locus_tag="IWM59_001962"
     CDS             7964..8680
                     /locus_tag="IWM59_001962"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9979989.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8686..9381
                     /gene="dapD"
                     /locus_tag="IWM59_001963"
     CDS             8686..9381
                     /gene="dapD"
                     /locus_tag="IWM59_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9979990.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9397..10398
                     /locus_tag="IWM59_001964"
     CDS             9397..10398
                     /locus_tag="IWM59_001964"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9979991.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10395..11756
                     /locus_tag="IWM59_001965"
     CDS             10395..11756
                     /locus_tag="IWM59_001965"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9979992.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11716..12867
                     /locus_tag="IWM59_001966"
     CDS             11716..12867
                     /locus_tag="IWM59_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9979993.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12842..14011
                     /locus_tag="IWM59_001967"
     CDS             12842..14011
                     /locus_tag="IWM59_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9979994.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14186..15565
                     /locus_tag="IWM59_001968"
     CDS             14186..15565
                     /locus_tag="IWM59_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9979995.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15846..16075
                     /locus_tag="IWM59_001969"
                     /pseudo
     CDS             15846..16075
                     /locus_tag="IWM59_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5256 a         2839 c         3428 g         4552 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacct
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc
     4801 gaaaatattt tttcatcact aaatagtgtt gaatttatgt ttttatgtat aaatagcttc
     4861 taatagttcg ggtattttgt ttttataacc atttttattc ttatttttaa aaaaatattt
     4921 cgaaatatac tagacaattc agaaaatttt gtttaatatt attccaataa aattcaaatc
     4981 tttatcaaat gaaattccaa taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga
     5041 cagaattgat aattggcgga gtcacagcaa gcacattggc agaaaagtat aagacacctc
     5101 tgtatgtgta tgatgaagaa aagatgagac aaacgatgct gtcattcaag cgaggctttg
     5161 tatcgaaacg ttttgataca aaagtgcttt acgcctctaa agcattccaa acgattgaga
     5221 tgctgcattt agttcgtgaa tatggattcg ggctagatgt tgtcagtggt ggggaaatct
     5281 ataccgcttt gcaagctgat tttcctagag atactatcta cttccatggt aacaacaaga
     5341 cgccagagga actgatctat gcacttgaaa atgatttgtt gcatattgtt gccgataatt
     5401 tgatggaagt tgaattatta gcagaattaa gccaacgcta tttaaaaaaa atttgtatca
     5461 tgcttcggtt gaatgtcgga gtagaagcac atactcatga atatatcgtc actgcgcata
     5521 ttgattcaaa attcggcatg agctatgaaa gtaaagagtg ccaacaagcc cttcagttga
     5581 taaaagaaag cagctatcta gagttagaag gatttcatgc tcatatcggg tcacaaattt
     5641 ttgatgttac tgcttggtta gctgaaatcg acaaattgac gaattactta accgattttg
     5701 accaacctct ttccttgaat cttggcggag ggtttgggat tcgttataca caaaatgatc
     5761 agccgatccc agtcgaagag gcactaaaaa aactgattga atatacggaa gaagctttgg
     5821 aaaaacgaaa actgaccatt cgtcaattac tgatcgaacc gggtagaagt attgttggag
     5881 aagctggtac aacactatac acgattggtt tcatcaaaaa gacaccacat aaaaaatatt
     5941 actttgtaga tggcgggatg acagataata tccgtccagc tctgtatcag gcagaatacg
     6001 attgtgattt agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa
     6061 aaatgtgtga atcaggcgat gtggtaatta aagaagctta tttgccgaaa gcagaatcgg
     6121 gtgatctttt agctgtttat tccacgggag cttatggata ctcgatgagc agcaactaca
     6181 atcgagcgac acgaccagca gttgtttttg tcaataatgg ccaatcaaga ctgattgttc
     6241 gcagacaagc attttctgat ctactaaggg gggaagtttc ctatgatcct tcataaatat
     6301 aatggctgcg gaaatgattt tatcttatta gattacgcag aagacacaga ttatagtcaa
     6361 ttagccgctt ctttatgcac aaaagaaaaa tatgatatag acggattgat cgccgtaaaa
     6421 atcgatccgt tggaaatgat ctattacaac aaagatggca gccgcgcgcc gatgtgcgga
     6481 aatggcattc gctgttttgc tcgatacgta aatgatcaga acatgatgaa tgaacaggaa
     6541 ttcaacgtag agacattagc aggcacaaga aaaattacgg tgttggatcc ctcacctttt
     6601 tattgcagcg tagatatggg agtgccagat gattctccgg aaaaaatcaa agctaatcaa
     6661 acttatccaa taaaaaacca ggaaatagag atcaatggtg aaaaagtaac gatcactagt
     6721 ttatttttag gaacgattca taccgttgtt tttgttgaaa atgcgcgtga ggaagtctgg
     6781 agaaaaagag gtgaactgat ctgtcatcat cctttgttca aagaaaaaac caatgtcaac
     6841 tttgtagaag tagtcaatga acaagaattg atcgtccgta cctatgaaag aggagtaggt
     6901 tggacacttg cttgcggtac aggttgctgt gcctcttatg tggttgctaa ggaaactggg
     6961 aaaatttcag cagatcaggt agttgttcac ttggaacaag gagacttgat gatctctggt
     7021 gaggatgcga tacaaatggc tggcccagca gttcatgaat ggacaagaat attggaggaa
     7081 tgaagatgat aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag
     7141 tagattatgc tggtttggaa aaattgatcc aattccattt agatgaacaa acagatggat
     7201 tattagtttt aggtacaaca ggagaatctt ctacgctcac acaatctgag gaggagcaga
     7261 tccttcaact gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa
     7321 cgaataatac gaaagaaacg attgaaaaag cgaagcattt tgcttctttg ggggcagacg
     7381 cacttttggt catcacccct tactacaaca aaacaagtga tgcaggacta gctgctcatt
     7441 ttacagcaat tgcagaagcc tcaccgattc cgcttatttt gtataatgtt ccttctagaa
     7501 caggcatgtc gattccaatc catgtattag taaatttagc agaacatcct aatatcattg
     7561 gtttgaaaga agcctcagga gatatggctt atgtgatgga tgccgctcgg ttgattggag
     7621 aagaattctt cctttacagt ggaaatgatg atttgatcct accagtaatg agtgtaggag
     7681 gaagcggtgt aataagtgta tgggcgaata tccaacctaa aatagtccat gaattagtaa
     7741 aagatacgca ggacggaaga tggcagcaag caaaagaaaa gcaattaaat gcacttgaac
     7801 tgatccatgc cttgttcagt gagaccaatc ctattcctgt caaagcagcc atgtcattat
     7861 tagacttacc ttccggacct ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga
     7921 aacaattagc tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat
     7981 tggcagtgga cagatgggaa atagagtcga agaagttgca caagaaagag gagatacggt
     8041 ccttctttta cggacggcac ctaaagaagc aactacgctt gcttttacgg aactgccaga
     8101 actgcttatt gatttttctc atccagataa tttagagaaa atcctttcgt atagcttatc
     8161 atatcaactt ccgttagtca ttggcacgac aggttataca caatcacagt tccaagaaat
     8221 aaaagaacat gccaaacatg tacctgtgat gtatagtgct aatttttcgt tcggcatcat
     8281 ggtgatgaat caactaatca agcaagcagc tgcaatgttg caaggctggc agatcgaact
     8341 gattgaaaaa catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct
     8401 tcaaacaatc caagaagtgc aaaagctgca gcccgtatac aactggcaag ggaaaaaaag
     8461 agaagaaaac caaattggtg tacacagtat tcgtgcaggt tctttacctg gtgaacatga
     8521 tgtattgttt gctgctactg atgaaatatt tacgatcaaa catgaatctt tttctaatcg
     8581 aatctttgct gcaggcgcag tagaagcagc ggactggttg aaagatcagt caccaggtta
     8641 ttacaaatta gaagatttat taatggacaa aggggtgtaa aaaacgtgga agctcaagaa
     8701 atcatccgct acattcaaac ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta
     8761 accgagccaa tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt
     8821 tttggtgact atgagacgat tgctccggtt ttagaagctg aatcagaaaa aatcattgaa
     8881 atcgaaatcg aaactgctgc aagatacagc gcagttcctt tgctagatat taaaaaaatt
     8941 aatgctcgta tcgaaccagg tgctattatc agagatcaag tgtcaatcgg caacaatgcc
     9001 gtgatcatga tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt
     9061 gatatgggtg ctgtcttagg tggacgagcg acagtgggca aaaactgcca tattggtgca
     9121 ggagctgtct tagcaggtgt gatcgaacca gcatcggcca aaccggttat cgtagaagac
     9181 ggcgtactgg taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca
     9241 gtcgtggcag caggagcagt ggttttagaa gatgtggcag cagaaactgt tgtcggtgga
     9301 atacctgcac gtgtattaaa aataagtgat gatcagacaa aagaaaatac agcattgatt
     9361 gctgcgttac gcgaattata gaggagatgg agaaaaatgc gcgaaataga tacctgtatt
     9421 gtgggggcaa ccggattagt cggccaaacg atgctcaaag tgttagaaga atatgatttt
     9481 ccagtaggac gattgaaatt attagcttca aaacgttcag ccggtaaagt ttgtgtattc
     9541 aagaaaaaga aacacactgt acaggaattg acagaaactt catttgaagg atatgattta
     9601 gcgctatttt ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag
     9661 ggactatttg tcatagataa ttcctcagca tggcgggaag attcggatat tccattaatc
     9721 gttccagagg tcaatctgga agactataca ttgaatcggc tgattgcaaa tccaaattgt
     9781 tcaaccattc aagcggtact tccgcttaaa gctttacaag aagcctttgg tatccagcga
     9841 gtcaattatt cgacctatca agcagtttct ggttccgggc aaaaaggcat tgaagatctt
     9901 catcgtacgc aaaatgacga ggaaccagtt ttgtatcctt ataatattag ccaaacggtg
     9961 attccggaaa tcgatcagcc tttggaaaat gggtatacaa aagaagaaca gaaaatgata
    10021 aatgagaccc gtaagattct gcatcagcct gctttgccag tatcagcaac ctgcgtacgt
    10081 gtgccaatcg aaaatggaca tggagtctct gtggcagttc aactgcaaaa agagtttacg
    10141 acggaagaaa ttagagagtg tctcagccag tttccaggga ttcaattaga agatgatcta
    10201 gcagcacata aatatccgac gagcattctt gctagaggaa cagatatggt ttatgttggg
    10261 aggatcagaa aagacacttc tttagagaac ggattgttgt tctatacgac tgccgataac
    10321 atacgcaaag gagccgctgc gaatgccgta cagattgctg cagctttaat gcagaaaata
    10381 gcggaggtgg caagatgagt aatacgctga aagtagcgaa attcggcgga acatcgatgt
    10441 ctcatgaaga gcaattcaaa aaagtccgta caattgttct ttcagatccc aatcgccaag
    10501 tgatcgttgt ttctgcttta ggcaaacgta cgtcagatga tgacaaagta acagacttac
    10561 tttatttgat ttatgcttac cttcagcatc atgttgattg gacacccctt tggaaaagga
    10621 tttgtgatcg tttcattcaa gtaagagatg ttcttgccct gcagttacct attgaagatg
    10681 aactagcacg catacagaaa caactggaag acgaaaagat aactgaagat tatttagtaa
    10741 gtagaggtga atatctgact gctcaactga tggctgagta tcttggttac cagtttattg
    10801 acgcaaaaga cctgattttc tttgactatg atgggaaaat cgatcaggaa aaaacagcac
    10861 aggaaatcac aaaagcattt aaaagatatc cgaaaatggt tgttccaggg ttttacgggg
    10921 cgaaccctgc aggaaaagaa aaattattgg gccgtggggg aagcgacatt accggcgcga
    10981 tactggcgaa gatgttgcaa gcggaaagtt atgagaattg gacggatgtt tcaggaatca
    11041 tgatggctga tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc tcttttagag
    11101 aactgagaga gatggcttat atgggggcta acgttcttca tgaagcagct gttttcccag
    11161 ttcaagaggc tgatatcccc atccaaataa aaaataccaa tgatcctttc gctcaaggga
    11221 caaaaatatc gaatcacgaa atagaaaagg aaaatggact gacaggaata gctggacgca
    11281 aggattttct ttccatcact ttattcaaac gtcatatgtc cgatgaaatc gggtttatct
    11341 ggaaagcaat gagcattttt gctaaacatg agatatcaat cgaacatatt ccttcaggga
    11401 tcgacaatat cggagtgatc gtctcagcag aagcgattgc cgataagtta tttctcatca
    11461 cgaaagaatt aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt
    11521 tgatctctgt cgttgggggt ccgcataaag aattgattgg attatctggg aaagtgttat
    11581 cgatattgaa caaattagaa attcgcacat cgatcttatc acaaggagca caggagttga
    11641 atctgatcat cggtgtaccg aataatcaat atgaaacagt agtgaagggt atctatgaag
    11701 ggatggtgaa tacggatgct gaaagaatat cgaatggcac tccatcaaat tcctgaaatc
    11761 gggtttcatg aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa cagcgaggcc
    11821 gttatccatg aaatcggcga aacaggacta ctcctttatt ttgataatca gcaagaagaa
    11881 acgattgctt ttcgaacaga tatcgatgca ctgccagtca aagaagaaac gaatctctct
    11941 tttgcttcta agcatgaagg ttatatgcat gcatgtggtc atgacggaca tatggcgatg
    12001 cttttagggt tgactgactt tataactaaa cacattgagg atcttattta taatgtagta
    12061 ctgatctatc agccatcaga agaaatttcc ggtggcgcac aaagcgttat tcagtcagga
    12121 ttgttggata aatatgaagt acaagcaata tttggctttc atctatggcc tggactagct
    12181 aagggagaag ttttctctcg accaggacct ttgatggcac aaagcagtga aacagacatt
    12241 atcattaaag gccagtctgc acatattgct tctagtgaaa aaggaatcga tagtctggag
    12301 gcagctgtac gatttttgaa gaatgtttat gattttgacc ggcaattacc ggcagagtgg
    12361 aatcatctaa tgaaatttgg ccaaatccaa ggtggaacga tccgaaatgt tttagctgga
    12421 gaagttattg tatccggaag tatccgttct tacagttcag atatgcaaaa ttatttgaag
    12481 aggggattag accatttagc agaacagttt cactctacag ctactatttc ttttcgctat
    12541 aatgacggct atccagctgt aatcaaccat agaggcttat atgcagcact gttagattca
    12601 aacaaactac atgaattaga tgctccagtt cttcaagccg aagattttgg catatatacg
    12661 caacaatatc catgtgtatt tttcttttta ggtgtcggtg atacccctgc tttacatgat
    12721 ccgacgttcg attttgatat ggatgtttta gaaaatggtg tagaatggta caaaacgatt
    12781 ctttttacag aaaatcttct tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat
    12841 aatgatgaat ttatccaatc gatttagtcc taggctttct agaatagaag tatcaaaaat
    12901 acgacaattc gatcagcaaa tatcgtcgat accagatgtg atcaaactca ccctgggaga
    12961 accggatttt ccaactcctg aacatgtcaa gcaagcagga atagcagcga ttgaagaaga
    13021 cttttctcac tataccggaa tgagaggatt agaggaactg cgggaagcgg cttgtatatt
    13081 tcagcaacag cgttatggat taacttatga tccgcaaacg gaagttttga cgacagttgg
    13141 tgcaacggaa gcgattgcct ctgcgttgtt atctgttttg gaagaaggag acaaggttct
    13201 tattccagcg cctgcttatt caggatatca gccattagtg gaactagctg gtgctgaatt
    13261 gatcccgata gatacttctg acacagggtt tgtctgtcag ccggaacaat ttgaacgagc
    13321 atttgaacaa tatggatcag ctgtcaaagc agtcatttta aactatccta acaatcctac
    13381 tgggacgaca ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa aaaaatatcc
    13441 ggtatttgtt ataagtgatg aagtatatgc tgaattgact tatagtggaa cgcatatgtc
    13501 catagctact tatcttccag aacaaacaat cgtgataagc gggttatcca agtcacatgc
    13561 gatgactggt tggcgagtgg gctttatttt tgctcaaaaa gcccttattg acgaactaat
    13621 taaagtccat caatatctgg tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga
    13681 agcgctaata aatggggtga aagatagcga gaatatgaaa gagcaatatc aaacacgaag
    13741 agactatctg gtccagcaat tggcgccttt aggatttgaa gtgacccagc caaacggtgc
    13801 attttacttg ttttgcaaac tcccagctgc cattcaaact gactcttggc agttttgtgt
    13861 agaattagcg gaacaggcaa aagttgcgtg tatcccagga attgcatttg gcccagaagg
    13921 agaaggttat atccggattt cttatgctag cagcatggaa aaattacatg aggcctgcaa
    13981 acggatcaaa gactttttaa cagaacagtg attcatagat tttatcaaaa aaacaatcgt
    14041 taattttgcg gtatattctg caaggagacg attgtttttt aaatttagtt caaatgaacg
    14101 cgtttctttt gattcataac taatgtaatg ttaagtttaa attgtttaga atgattttaa
    14161 aagaatattc aaggaggttt catcaatgga aaaaatcaaa gttgtcattg taggtgcatc
    14221 tcatggaggg catcaatcga tcttggaact attgacacga tacggtgaaa atgtagatat
    14281 cacgctattt gaagcaggag actatatttc cttcatgtca tgtggtatgg agctgtattt
    14341 agaagacaag gtaacagatg taaatgatgt acgtaatttc agaccagaaa actttccgca
    14401 atccaatgtt catattttaa ataatcacga agtaaaaacg atcgatgccg ataaaaaaac
    14461 agtaacggtt actagcgcat cagataacga aacaaaagaa tatccttatg acaaattgat
    14521 tttgagttct ggtgtcaaac caaacaatct gccagtacca ggagcagatc tagaaaatgt
    14581 ctacttgatg cgtggataca attgggcaac caaaatcaaa gaaaaattaa atgatccggc
    14641 tattaaaaaa atcgctgtcg taggtgcggg ttacattggg atcgaagcag cagaagcaag
    14701 ccgaaaagct gggaaagaag tcgtattgct tgatgtgatc gatagacctt taggcactta
    14761 tttagatgct gagatgacag atattttaga acaacaccta aatgaaaacg gtattgaagt
    14821 tttgacaaat gcaaaaatcg aagcttttac tggtaacgga aaagttgagg caatcaaaac
    14881 aagtgaaaaa gaaatcccag cagatctagt cattcaagct gccggtgtga aggcaaatac
    14941 agaatggtta aaagggatcg tcgacttaga tgaacgtggc tggatccaaa caaatgaata
    15001 cttgcaaacg aatcttccag atgtgtatgc agtaggagat gctacacttg cttattctat
    15061 cccagcacgc aaaaaaatgc cgatcgcttt agcgactgtt gcacgtagag aagcacgtta
    15121 tgtagtaaaa catctttttg aagaagtacc aaacaaaccg ttcggcggag tcgtaggttc
    15181 ctctgcatta agtgtctttg actatcattt tgcagcaagc ggattgaata gctttacagc
    15241 agcaaaagca ggagtagatg tacgagtgtc ttattatgaa gatacgttac gaccaaaata
    15301 tgtaccagaa gaacaaggaa atccaaaagt gtccatccaa ttattctcgg atccaatgac
    15361 ccatcaattg ctaggaggag cagtattatc tacctatgat gttacaggtc aaggaaatgt
    15421 attggcacta gcgatccaaa acaaactgac tttagaagaa ctagcagaag ctgacttctt
    15481 cttccagcca ggctttgacc gacaatggag catgttgaat ctagccgctc aacaagcgtt
    15541 aggagaagaa ccatttgtag aataagaaaa cgaaatattt gaaaagaggt tgtgaaaaag
    15601 ctgccttgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
    15661 ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattttca aaaaagggac
    15721 tgtgacacga tttgtgtacc gacccccaaa agttagacca aaaaatctaa cttttggagg
    15781 tcggtatttt ttatggttaa atatactttt gaacttaaat taaagatcgt tcatgattat
    15841 ttggatggca aaggcgattg gaccgtaaaa atccagatca agagattgaa gcggaaatgc
    15901 ttaaaattcg ggagaagcac aaggattatg gctgtttacg gatgaccaat gaactaagaa
    15961 accgcggttt ctctatcaat aaaaagaaag ttcagcgctt gatcaagaag ctaggaataa
    16021 aagtaacggc atacacgcgt aaatcacgtc gctacaattc ttatcgagga aaagt
//
LOCUS       DACDYM010000061        15931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDYM010000061 DACDYM010000000
VERSION     DACDYM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.061"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..1151)
                     /locus_tag="IWM59_001970"
     CDS             complement(12..1151)
                     /locus_tag="IWM59_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9979996.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1550..2848)
                     /gene="eno"
                     /locus_tag="IWM59_001971"
     CDS             complement(1550..2848)
                     /gene="eno"
                     /locus_tag="IWM59_001971"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9979997.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2964..3719)
                     /locus_tag="IWM59_001972"
     CDS             complement(2964..3719)
                     /locus_tag="IWM59_001972"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9979998.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3823..5013)
                     /locus_tag="IWM59_001973"
     CDS             complement(3823..5013)
                     /locus_tag="IWM59_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9979999.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5123..6124)
                     /gene="gap"
                     /locus_tag="IWM59_001974"
     CDS             complement(5123..6124)
                     /gene="gap"
                     /locus_tag="IWM59_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9980000.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6165..7202)
                     /locus_tag="IWM59_001975"
     CDS             complement(6165..7202)
                     /locus_tag="IWM59_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9980001.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            complement(7405..8739)
                     /gene="rpoN"
                     /locus_tag="IWM59_001976"
     CDS             complement(7405..8739)
                     /gene="rpoN"
                     /locus_tag="IWM59_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9980002.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8799..8870
                     /locus_tag="IWM59_001977"
     tRNA            8799..8870
                     /locus_tag="IWM59_001977"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8832..8834,aa:Arg,seq:ccg)
     gene            complement(9062..9946)
                     /locus_tag="IWM59_001978"
     CDS             complement(9062..9946)
                     /locus_tag="IWM59_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9980003.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(10033..10932)
                     /locus_tag="IWM59_001979"
     CDS             complement(10033..10932)
                     /locus_tag="IWM59_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9980004.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(10949..11455)
                     /locus_tag="IWM59_001980"
     CDS             complement(10949..11455)
                     /locus_tag="IWM59_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9980005.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(11644..12264)
                     /locus_tag="IWM59_001981"
     CDS             complement(11644..12264)
                     /locus_tag="IWM59_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9980006.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(12340..12777)
                     /locus_tag="IWM59_001982"
     CDS             complement(12340..12777)
                     /locus_tag="IWM59_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9980007.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            12940..13770
                     /locus_tag="IWM59_001983"
     CDS             12940..13770
                     /locus_tag="IWM59_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9980008.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            13754..15121
                     /locus_tag="IWM59_001984"
     CDS             13754..15121
                     /locus_tag="IWM59_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9980009.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            15140..>15931
                     /gene="yidA"
                     /locus_tag="IWM59_001985"
     CDS             15140..>15931
                     /gene="yidA"
                     /locus_tag="IWM59_001985"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9980010.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAI"
BASE COUNT         4307 a         3333 c         2938 g         5353 t
ORIGIN      
        1 ttaaaaaaat cttacgctaa gtaggataaa ataattcctt gatatttttc gatcatatcc
       61 aaataattat cgatttcaac atactcatct acttgatgag gcaaagtaac gacaccaggc
      121 ccaaagacga caaagtcgaa ggaatggtta gctttcgtaa attcggctgc atccgttgtt
      181 gctgccgctc caactaaagg taatggctgg ctgaattgtt gctgaatgca atgaatcaaa
      241 ggagaatctg gatcagcttt gacaggtatt ttgttgtaat cgatcatcaa ttccaaatga
      301 tagtctgtct cttgattcaa ttcattgacg attgattgca gcaatgcaat gattttatca
      361 tttgggtatt ctggaatcga tcgaatgttc ccttggagct gcgcatggct tgggatgctg
      421 ttgacttgat tcccgccact gatcaatgta acattatgga ttgtttttcc taggacagga
      481 ttctcgattg tttcagcgag gtgattcatt tcagcatttg ctttcgtaat gaattcattt
      541 aggtgattga ttgcattata accttggtct ggcatagagc tatgcgcttc ttttccgtgc
      601 gaggtgactg tatagttgat tgatcccatg tgcgtataca tcaaactgta attggttggc
      661 tctccgatga tcaaggcatc taagtcatct acatagcctg ctttcgtgag ctgttcccca
      721 cctagttcac ccacttcttc tccgacagtg gctaataatt tgactgttcc gttaaatggt
      781 tttcctgact ctttcagttc gatcatggca atcaccattg ctgccaagcc actcttcata
      841 tctgtagcac cacgtccgta caaacggttc ccatggattt ctgctgcaaa gggtgcatag
      901 gtccaagacg attcatcacc agccgccacg acatccatat gtccagatag gcctaacact
      961 tttcctgatt ggcccttttg gtaacgagca atcaaattat ctctaccatc tctgtaagaa
     1021 acaatctctc ctgttatgtc atgctttgcc aaaagcttat tcaaataggc tgctacttca
     1081 ccttcgtttc cattcactga tttaattcgg ataatctctt gtaaaatcgc gattttttct
     1141 tcttttttca tctaatcctc tcccttgcca tttcttttta ttgtatttga gtgtgctcga
     1201 attttcaaat aggagcaacc aaagtaaaaa tgaagaaaga gagccacttt atgaaaaata
     1261 tgctgctact tccctcctat tttatttata cgagaggact tgcccataga ttcctattct
     1321 actgttctat ccttaaaagc tcttatatga tgctagttat tgttagcttt cctttttcaa
     1381 ggatgatctt ttcttgtacc attctttgta aacatcatat acagaagcta gttgcaaaaa
     1441 aactctctac ctgctgatat ttcagcaagt agagagttcg attacttaac taagtgatca
     1501 tactcgacta tctttcaata gtcgatcatg tatcttcatt agaaaagaat tatttgtttt
     1561 ttaagttgta gaaagatttc aaacctttgt attctgcaac ttcaccaagt tggtcttcga
     1621 tacgtagtaa ttggttgtat ttagcaatac ggtcagtacg tgatagagaa ccagttttga
     1681 tttggccagc gtttgttgca acagcgatat cagagattgt tgaatcttct gtttcaccag
     1741 aacggtgaga tacaactgct gtgtagccag cttctttagc catttcgata gcttcgaaag
     1801 tttctgttaa tgtaccgatt tggttcactt tgataaggat tgagttagca attccttttt
     1861 cgataccttc agataatttt tgagtgtttg ttacgaacaa gtcgtcacca actaattgaa
     1921 ctttgtcgcc taatacgtca gttaatttct tgaatccgtc ccagtcgttt tcgtctaatc
     1981 catcttcgat tgagatgatt ggatatttag aaactaattc ttcgtagaat ttgatcattt
     2041 cgtcagttgt tttttcgcct tcgcctgagt cagctagtac gtaaacacct ttttctttgt
     2101 cgtagaattc tgaagaagca gcatccatag caagaacaac gtctttacca ggtacatagc
     2161 cagctttttc gattgcttcg atgattactt caaaaccttc ttcgtttgaa ccaaggtttg
     2221 gagcaaatcc accttcgtca ccaacagaag ttgctaatcc gcgagctttc aagattgaag
     2281 ctaatgcgtg gaatacttca gcacccatac gtagagcttc tttgaatgtt ggagcgccaa
     2341 caggcatgat catgaattct tggaagtcga ttgagttatc agcatgtgat ccgccgttga
     2401 tgatgttcat cattggtgtt ggcaatactt ttgtgttgaa tccgcctagg tagtggtata
     2461 aaggtacttc taggtagtca gcagctgcac gtgcaacagc gatagaaaca cctaaaatag
     2521 cgttcgcacc taatttacct ttgttaggag ttccgtctaa agcgatcata gctttgtcga
     2581 tagccatttg gtcacgaaca tcgtagccaa tgattgcttc agcaatgatg ttgtttacgt
     2641 tgtcgacagc tttagttaca cctttaccgc cataacgagc tttgtctccg tcacgtaatt
     2701 caactgcttc gtattcacca gttgaagcac ctgaaggaac cattccgcgg ccaaaagctc
     2761 cgctttctgt gtatacttct acttcgattg ttgggttacc acgtgagtct aagatctcgc
     2821 gagcgtaaac atcagtaata attgacatgt tttgtctctc ctttgagttt gttttttcct
     2881 aagggatttc cctcatatac gattttagtt aattttgctc attagtgcaa acatttcttg
     2941 catttttcgc aaattttcaa caattatttt acagcgtcta acaatgacaa gaatgaatct
     3001 gcttgcaagc ttgcgccacc gactaaagca ccgtcaacat tttcttttgc catgtattca
     3061 gcaatatttt ctggttttac agaaccgccg tattggatac gaactgcttc tgatacttct
     3121 tttccataaa gtttttcaac tgtttgacgt acaactccac aaatttcgtc agcgatttgt
     3181 gcgtcagctg atttaccagt accgattgcc cagattggtt cataagcaat aaccatgttt
     3241 gacacctgtt cagctgaaag atcagctaaa ccagctgtaa tttgtccttc gatccattca
     3301 gcagttttgc ctgcttcata ggtttctaaa gattcgccgc agcataggat tggtgtcata
     3361 ccatttgcaa agatagcttt cgctttttta ttgatgtctt cgtctgtttc atggaaatat
     3421 tcacgacgtt ctgaatgtcc gatgatcaca taatgaacac ctagatcagc aagagctgct
     3481 ggtgaagttt caccagtgaa cgcacctgag ttttcccagt agcagttttg tgctgagatt
     3541 tttagttctg taccttcagc tgatgtcatt aattcttgta agaataatgc aggagatccg
     3601 atgactgaat caactttgtc atttgaaggg attttgtttt ttactgcttc agcaaattct
     3661 tttgcttctg atgcagtttt gttcattttc cagttacctg cgataatagg tttacgcatt
     3721 gaggaacaca ctccttaatt tattgaaaaa tggctaggga gtcatgaaaa ctcccttcac
     3781 cttttgttcg attactcgtc agaattgagc aaacttggtc gtttatttat cgttgattgc
     3841 tgctagtcct ggtaattctt taccttcaag caattccaag cttgcgccgc cacctgttga
     3901 gatgtgtgtg aatttgtcag caaatcctaa ttgttctgct gctgcggctg agtctccgcc
     3961 accgatgatt gttgttgcgc cttctaagtt agcgattgct tcacaaacac cgattgtacc
     4021 tttagcaaag ttactcattt caaatacacc cataggtccg ttccaaacaa ctgtttttgc
     4081 accttgtaat gtatcagcaa ataattttac tgatgctggg ccgatgtcta aagccatcaa
     4141 accatcagga atgtctcctt cgattacttg tgtttcaaca tcatttgaaa attctggtgc
     4201 acagacattg tcgattggca agacaagttt gtcgcctgct ttttcgatta attcttttgc
     4261 tagttccact ttatcagctt ctactagaga gttaccgatc ttgattcctt ttgcttcgta
     4321 gaatgtgtaa gtcattccgc caccgatcaa gattttatct gctttaggaa tcaagttttc
     4381 gattacgccg attttatcag aaacttttgc gccaccaagg attgcaatca ttggtcgttt
     4441 tggttcttct actgcttcac cgatgaactt gatttctttt tccatcaaga atccagcaac
     4501 tgttgggata ccagttgaag cgatacctac gttagaagca tgtgcacggt gagctgtacc
     4561 gaaagcatcg ttaacaaata catcgcctaa tgaagcccag tatttaccta gttctgtatc
     4621 atttttgctt tcttttttac cgtcaatatc ttcaaatcgt gtattttcga atacgacaac
     4681 gtcgccgtca ttcatgttgc ggattgcttc ttctaattgt tctccgcgag tttcaggtac
     4741 gaatgttact tctttaccta ataattcgcc tagacgtttt gcaactggag ctaaagattt
     4801 tccttcttta tcttcttctg tttttacacg tccaaggtga gagaaaagga ttgcttttcc
     4861 gccttgttct agaacgtagt tgattgttgg caatgctgct ttgatacgag tgtcgtcagt
     4921 gatcacgccg tctttcaaag gaacgttgaa gtcaacacgg acaagaactt ttttgtcttt
     4981 taaatcgata tctttcactg ttttcttagc cattttattt cctcctgaat aaacttatta
     5041 tcaaaaaaag cggaagaagc gcgattgctt ccccgctttt aaatgtacat gtctataatt
     5101 tacacttgta cgtttgtcta tcttataagt tagcgaagta ttctaaagta cgtactaatt
     5161 gtgcagtgta tgacatttcg ttgtcgtacc aagcaacagt tttaactaat tgtttgtcgc
     5221 caactgtcat tactttagtt tgtgtagcat caaataatga accgtaagtc ataccaacga
     5281 tatctgaaga aacgatttcg tctgtgttgt atccgtaaga ttcgtttgaa gcttctttca
     5341 ttgctgcatt gatttcgtca acagttactt ctttttcaag aactgttact aattctgtta
     5401 atgaacctgt tggtacagga acacgttgag cagctccgtc taatttaccg ttcaattcgg
     5461 gaattactaa accgatagct ttagcagcac ctgttgagtt aggaacgatg tttgcagcag
     5521 cagcgcgtgc acggcggaag tcacctttag gatgaggtcc gtctagagtc atttggtcac
     5581 ctgtgtaagc gtggatagtt gtcattaatc cttcaacaac accaaatttg tcgttcaatg
     5641 ttttagccat aggagctaaa cagttagtag tacatgaagc acctgagata actgtttctt
     5701 taccagttaa tgtttcgtgg tttgtgttat aaacgattgt tggaacatcg tttccgccag
     5761 gagcagagat aacaacgcgt ttagcaccag ctgttaaatg tttttcagct gctgatttag
     5821 aagtgaagaa accagtacat tctagaacga tatctacgcc tagttcgccc catggtaatt
     5881 cttctgggtt gcggttagct aatactttaa cttcttttcc gtttacgttg aatgaacctt
     5941 catgaacttc aactgttccg ttgaaacgtc cttgagttgt gtcgtatttt aacaagtgtg
     6001 ccaacatttt agcatctgtc aagtcgttga ttgctactac ttcgattcca tctacatctt
     6061 ggatacgacg gaatgctaga cgtccaatac gtccaaaacc attaatacct actttaactg
     6121 tcatttaaga tttcctcctt atgaaaatca aaaaaatatt tattttaaag ggttacccct
     6181 tttaaaatct catttgcagc agcttcatca gtgatgagcc acgtttgttt tggtgcattt
     6241 ttcatataag ctcgaattgc tttagctttt gattttccgc ccgcgattgc catgatgatc
     6301 gggacttttt ccaaatcttg caattctatg ccgattctag ggaccttata aacgacttca
     6361 cctttttcat tgaaaaagta gccaaaagat tccgcaacag catttgcttg tttcagcatg
     6421 actaattctt tttcagtcat cttacgacgt gctgccatgt gtaatgcacg cccaatacta
     6481 tgaataacac aattcgcttc actaatcaag tttaagactt cttggataga tggctcattg
     6541 agtaaagaat tgtaagttgc cagacttaat tgttccggaa cataaagtgc gcggtggtta
     6601 ccgtttgctt tagcagccat tcttgcactg acagaatttg cctgtacagt tatcgcttca
     6661 ccaatcccgc ctcttgcagg gacaaacagg ttatgccgtt tcttattttc cagattagac
     6721 aattgttcgg ctaccacagc catagtagtt cctcccatca cagcgatgat attctcgcct
     6781 tctggaagta gtcggtctaa ggcttcgctc agcgcttcgc cgaatgcatc agccactttg
     6841 gtctgctctt cgctgtttcc tgaaacaacg atacagcgtt ggttaccaaa atattctgcc
     6901 aactgttgtt cggtttgatg cgtacctaaa agctgatcca tgaagttttc aaggctttga
     6961 taaacttctt cgcccttagt tgtcagcgtc ataccgcttt ttgaggtgtc gatcaaattc
     7021 agatttttca aaaggtctgt ttctgttcgt aagactcgtt ctgtgatccc catactttcc
     7081 gataaacttc gacgcccaat cggctgcatc caatagatat ttcgtaaaat tttatagcgt
     7141 tcttgcaaca cttcgataat atctggtgca acagattcaa tcatttttat ttcatccagc
     7201 atagcgatct ccttagttgg acactttacg tccaatgtag acttaaaacg acccatatac
     7261 gataaaaaaa gaaattaact attcgaattt cacatcaata aagggaaatc tcaacctcac
     7321 gactgtaagt ctaacagttt cgctctcgtt tttcaactaa aaagactaga aaaagtgaaa
     7381 tactttttct agtcttttga ctgattagtt atcataacgt tttctttttg atgaactcgg
     7441 gattcctaat aaatcacgat attttgctac tgttcgtctt gagatcgcta tctcatcttt
     7501 tttcagcaat tcaaccaatt tctggtccga caacggcttg tttttgtctt cttgatcgac
     7561 taattcctgc agctttttct tcgctgtatc tgctgaaagg tcttctgtct gttctgtact
     7621 attcgtagga attcgtgtag tgaaaaattt ttttaattca aatacaccga aatctgtttc
     7681 caaatatttc ccgttgactg cacgactgac cgtggattca tgcacatcta tctcagttgc
     7741 aacttccttt aacgtaagag gcttgattgg acgttccttg tcgataaaga aagctttttg
     7801 ccgggaaaca atgacttgag caacccgcaa aattgtatcc ccacgttgta ggatcgtttt
     7861 cttcaaccat tcgaattcct gtttccgttc ttttaaataa ttttcagttt ctttatctgc
     7921 ttgttgtgac atttgtttga agtaactctc ttgaaagcgt atatttggct gattttttcg
     7981 gtttgagata acttgtacat ttttatcgtc gtccactaat acccggacat caggaataat
     8041 gtacaattcg ctgctacggt cgaaaatccg gccagggctt ggactcaacg tttgtaaata
     8101 gtcgaatatc tgctgaacag catgtagatc aacagaaaaa cgttgtgcga tttctttcca
     8161 ttttcgctcc accagttcct caaaacattc ttccaacacg atataagcta attccggcgc
     8221 ataatcatct cgttctgttt gaagcatgag acattcttgc aaactacgtg cacctactcc
     8281 agctggttct aattgctgaa tcagagtcaa cgcatcgagc atttggatag gcgtagctcc
     8341 aatctctttt ccggcttctt ctaaactaat cgtcaaaaat ccgttcaaat cgatgtattc
     8401 tacaagataa agaacgattt tcctcaaaaa agtatctcgg taattcaaat ggacttgttc
     8461 gatcaatgaa tcaaataaag acccttttgt atcaggaatc tgatttaggt aatttgtctc
     8521 ttctccttca taagaagatg aagaagcttt tatgtaatct ggctgccatt ccggctcaac
     8581 aacttccacc aatggatttt ccattgcttg attctcaacg aatgccaaca gctcctcact
     8641 gttatattgt aaaacttgga tggactgttg caactgttgc gtcatggcta gtttctgtgt
     8701 ctgctgctgt ttttgtgaaa aattttgctc aaatttcatt tttttgtcca atccccttgt
     8761 aattttttat ctgtttagtt acaattatcc cagtgcttgc gtccttagtg tagtggatat
     8821 cacgtaagat tccggttctt gagacggggg ttcgattccc tcaggacgcg ttctactgct
     8881 attttagcat aatttacgaa tagttgcttc tttattttta agtgaaaact gtccgccaaa
     8941 gaaaattggt ggacagtttt ttgttgtacc tgtatagata ttgttaagcc tttgcaagta
     9001 aggtagttat gtgttcaact acttattaaa atgtgttatt tattaaagat acttgtctca
     9061 cttactgaac tggccgatga tcgtttctgc cataatagtt ctcgacagcc gttggatgcc
     9121 ctaataaatc cccaactgtc gtgatgcgat acccttgctc tttcagataa tcgatcactt
     9181 gaggaacggc acgaatcgtt tcaggataga tatcatggaa taaaataatt gccccatcgt
     9241 atactgttgc ttggatcttc tgcaaaatca aatcaggatt tcttgtcttc caatcttctg
     9301 aatcaacaga ccattcgatg atcggtcgct ggatgatcgt tgctcctaca ttcgtgacag
     9361 aaccataggg cggacgaaca aaagtggggg ttttgccagt gacttttttg atctcagtgt
     9421 ctgctttcgt cacttcctcc gtcatcgact gggcagaaag agtggccaga ttctgatgat
     9481 cgtatgtgta attgccaatc tcgtgccctg cttcagctgt ctgcttcacg atttcaggat
     9541 gctgcgccgt attttctcct aagacgaaaa aagtggcatg cacgtttttt tcttttaaga
     9601 ttcgcagaag ctgcggtgtt gtggtcgctc ctggtccatc atcaaagctg attgcgacgc
     9661 ttttgacagc cggatctcgg ctcgtttttg tactgtcttg tgttgattgt gatgactgac
     9721 tttgtacctt ttccgctgat tcttgaatgg cttctttgac gttagctgtt tcagttgatt
     9781 ggcttttaat ggttgattgt tctgattgtt tgaaagattg tgacgaatgc tccgaagagt
     9841 gtgtttgtcc ttttacttcg gtgatcaagg aggccaccga agtaatttcg aaaaacaaaa
     9901 gcacggataa agcaataata ctgataacta aataatttct tttcactata tctcttcttt
     9961 ctctttttct cttcttatta tttacttgta cttacttgaa aattgaaact gctagtcgca
    10021 actacttctc gtctaatgat ccaaataatc ttccgatgcc aaaatcgttt ttgtcacgga
    10081 cttaggaatg acaaattctg gtgtacccat ataaccagga gcaatttcgt attgagaaaa
    10141 agtcaaaact aattgatgct ctttgttcaa ataaaaactt cgtgtaggcg taacaagtgt
    10201 tggtttttct atcgtccctt cttgaatatc ttgctcagtc caatagtact tagatggatc
    10261 ttccttgact tgttcggcca tctgccgttt gacttctttt gtcaaaacag atagataggt
    10321 gtcatctttg aataaaagtg gaagtgagag ctggacactg tttttcttgt cgattgtgtc
    10381 gtatcttttt tcaacatggc tatctgctgc tgtttgggtg atctgccttt caatcacgag
    10441 aaaccggtta tcgtctacga ttttctgata atctcccaaa acttggtaat gatccgtttc
    10501 aaaatttcca tattcttttt cagctttttc aaaatctgct tgtccttcgc gaaagtattt
    10561 ttgatttaat ccatgaattg ttttattttc tttctcattt tgtgtattga tcttcggtac
    10621 ctgaatatcg attttttcag atcgatcagt aaagcgttcg cctaagatca attcaatgac
    10681 tgatcctaag atgggtaaat cttctgcaaa gcttcgaact tgactattaa aaagaaggct
    10741 acttgttgta atcattacga tagccaaaga tactccgaac ccttggagac gtctctttct
    10801 ccgctttgct tttataaatg attgctcttc gataataaaa cgctggcgga tctcctcttt
    10861 aagccctttg ggaattgctt gttctttata agttttcttt agttttttta aattttcttt
    10921 ttgggaatac atggcactct tctctccttc attctaggat ttgacgtaaa gcattcaatg
    10981 agcggtacat tttcgtttta actgtgtttt cattcatatc caagatagtc gcaatttctt
    11041 tcaaacgaaa tccttcaaaa aagtagagga tcagaatctc tttttccgga ctgtctattt
    11101 tatctaaaat catatggagc tctgcaacgg aagtttctaa aggtaactgt tgggggatat
    11161 cttgaatttc aaataagtcc acaggcgtcc ttttttgtct gcgccaacta tctaatgccg
    11221 tgcggatcaa tatccgataa aaccatgcac taaaatgctc gatctctttt tctttttcaa
    11281 aatagttcag ggctttatga aaactatctt gcatcacatc taaggcctct tctttatcat
    11341 gtacgtaatt catcgtgata cggaacattt tttgataatc agtttcgatg agtccattca
    11401 gcaggtgacg gtatttttcg atctgccgtt ttttcttata tccagtaaaa atcattctcc
    11461 actcctttct gcctcactta atatgacgta caaggtgcgc taaaagtttc aattttttta
    11521 ttttttatgt atttacttgg gatttttata tacaaaaaag aaggtgtgac aaaaatcgtg
    11581 tcacacctcc ttattctgat aggctgatcg tctctgtcac aacctctcgt aagaaaataa
    11641 tgtttattct ttttcagagt cagaaaattc ttcctcttca taatcttcat catcatcgtc
    11701 ttcgtctatg atacttaaat cttcttcgat atttcctggc aagtcttctt catcatctgt
    11761 cgggtcatct gctccgattt cttgcaagtc agaattataa gcagcgattt cttcatcttc
    11821 atcatcatcg tcatcaaaca gatcatcgtc atcttcttct gtcagatctg tatcttctgg
    11881 gtcatcgtca ttgtaatcga tcgcgtcttc atcatcattg ataaaggcgt tgactttctt
    11941 gcgtttacgg cgacgtggag tatcttcatc ctcttcgtcc attccatggt ttacttcttc
    12001 atcgatcgaa tcgatagcat accatgaacg caagccccaa cgattatccc ctaatgagat
    12061 aaagctgccg tcaatattca agtcagtata aaattgtgcg agtgaatcac gaatttcact
    12121 gtctgatttc ccaagatagt tttggatctg gttagctaaa tcagaaaaat ccatgacatc
    12181 gccacgttgt tctaagatcg cgtgtgctac ttcgatcatt gataattcgc ttttgtttgc
    12241 accttcaaat acactaattt ccaatcagga cacgtccttt cacagtctac tccttatcat
    12301 acaaaatcag acacaaaaaa tcaaactttt cttttatatt tacgctgtaa aatccaaggt
    12361 caaataatat tcgccgattt tttccggtcc catgaacaga tcataatcga ttcgaacctt
    12421 ccctcctgtg ggacggtctt tcaaactaac atgcagttct ttcgtaaaag tagcaatctg
    12481 gatcataccg tatggtgttc gataggatgt ttctaaccgt tctttatagc caaatttcaa
    12541 acgcatccgc atttctcctg cacggatcag ctgtacatga ccatctggaa ctaatttgat
    12601 cgttacagga atctcatcgc ccgtctcctc ctgaatctct ttataacgta tgtacagcgt
    12661 atcgccaatt gtcaccattt gccctttcaa atcaaaataa aaatcttgca cttccccttg
    12721 ctgttttact tttgtacgca atttgattga aatcggtgtt ccttgtgata attccattat
    12781 tcattctcct ttacttctca tagctacatt gttcctagtt tcttttgttt tgtcaaccaa
    12841 cttgtccatt tttacagaaa accttttttc caaaacaact ctttgatttt agagtataat
    12901 attgtagaaa atatgaattt atacaaagga gccggttgaa tgaaccctgc gattcgacta
    12961 gatcagcaca tttatcaaaa aaacgacttt tccccttttg cattaacaga tatattaacg
    13021 gaatattcta aagaacacca gcaaatcttt ttacatttct ggcaatatga ctgcacagtc
    13081 atcttaggta tgaaggatgt tcgaactcct tttctaagtg acgggatcca aacactgaaa
    13141 gatgcaggtt acgctccagt gatcagaaat tcaggtggat tagggatcgt atcagatgaa
    13201 ggaattttga atatttcttt gattttcccc caaagtgaca caaaaaaaat aaccatagat
    13261 gacgcttacg agcaaatgct cctattgact aaatacgctt ttccaaaggc accgatacaa
    13321 gcattcgaaa tcacccattc ttattgtcct ggaacttatg atttaagcat ccatggcaaa
    13381 aaatttgccg gtatcgctca gcgtcggatc aaaaatggga tttctgttat gatgtactta
    13441 agtgtcaacg gtaatcagga aaaacgcggg caagtcatga agcgatttta tcaatctgct
    13501 ttaaaagaag actttggtac aaatggctat cctgcagtgg atcctgctac aatggcaaca
    13561 cttgaagaac tgcttgacca accccttttt gtaaaagaag tcaaagaagc atttgcaaaa
    13621 gcttttgaaa agctgtattc tgcaagtgat ttacaggatt ctaccgactg gctaaaaaaa
    13681 gaaatccagc tggagcaatg gaacaaccaa atccagagaa tggaacaacg taataacata
    13741 aaggagctac attatgacaa ccccttataa atatcaaatg ctgcctatgg aaaaagtttt
    13801 tcgtgatcca gtccataact atatccatgt ccagcatcaa gtaatcttag atttgatcaa
    13861 ttcgaaagaa gtccaacgtc tacgacgcat taaacaatta ggcacttctt ctttcacttt
    13921 tcacggagca gaacatagcc gttttactca ttcattaggc gtttatgaga tttcaagacg
    13981 aatctgcgat atcttttctc gaaatttttc aaaagaaaaa ataggcaacg gaggctggga
    14041 tgatcaggaa cggctggtga ctttatgtgc ggcactgttg catgatgtcg gtcatggtcc
    14101 ttactctcat acatttgaac atatcttcca tacgaatcat gaagccatca ctgtagagat
    14161 catcacttct ccagaaacag aagtgtatca gattcttaac cgagtagaag aaggttttcc
    14221 tgaaaaggta gctagtgtga tccaaaaaac ttaccccaac ccacaagttg tccaaatgat
    14281 ttctagtcag atcgatgcag atcgaatgga ctatttgctt cgggatgctt atttcactgg
    14341 aacagaatat ggaacatttg atcttacccg aatattacgt gtgattcgtc cttatgaagg
    14401 cgggatcgct ttttccatga gtggcatgca tgcagtggaa gattatatcg ttagccgata
    14461 tcaaatgtat gtgcaagtgt acttccatcc tgtttcccgt ggtatggaag taattttgga
    14521 gcatctgctt catcgcgcac acgagttgta tcaagaagat gccagcagtt ttgaactcca
    14581 ttctcaatta cttctgccat ttcttgacgg aagctttact ttagaagaat acctcaaact
    14641 tgatgatggg gtcttgggca cttactttac tcaatggtct gaatctgctg atcctatttt
    14701 aaatgattta gctaaacgtt ttttgaatcg taaaccactg aaatctgcta ctttttctgg
    14761 caaccgggat tcaaaattag tccaagaact cactttgtta gtagaaaagg tcggatataa
    14821 tcctgtttat tacactgctg tgaattctag ctacgatcta ccttatgatt tttatcgccc
    14881 agaacaaggc agacatcgca cacaaatcga gattttgcga aatgacggaa ctttgataga
    14941 attatcacag gttagtcagc ttgttgctgc attagcggga caagaacaag gagatgagcg
    15001 atttttcttt ccaaaagaaa tggttgatcc gtccttgcgt gatcattatg atttatttga
    15061 cgaaacttat caagaatttg ccagccatat ccgtaatggc gcattgattg aaataaatta
    15121 aaatatattg gaggaaatta tgtctatcaa actagtcgct atcgacatcg atggtacttt
    15181 attaaatgag aaaagagaag taacaaaaga agtaaaagaa gcgattgccg ctgctgttgc
    15241 aaaaggtgtt tcgatcgttt tatgcactgg ccgtccctta ccaggtgtcc aggaacagtt
    15301 aaacgagttg aacctttttc aagataatga ttacgttatt acttataacg gggcactcgt
    15361 acaacaaacg aaatcaggta aaatcatcgc ccgtcacgga ctgacacatg aagatttcct
    15421 agagatcgaa gtgatggcac gccgtgtcgg cagtcatctc cattcgatcg atgacaaagc
    15481 gatctacacg cctaatcgag atatcagcgc ttatacgatc cacgaagcaa gtttagtcaa
    15541 gatgccgcta aaatatcgaa cacctgaaga aatgacacca gacatgaaca tcgtcaaaat
    15601 gatgatgatc gacgaaccag aaattttaga tgcagcaatt gcgcgtttac cgcaaacttt
    15661 ccgtgataaa tacacaacgg tcaaaagtgc gccctattac ttcgaagtac taaataaaga
    15721 agcaagtaaa ggtgcggcag ttgcgaatct cgcacagcac cttggtattg atcaagatga
    15781 aatcatggct atcggagata acgagaacga cttatccatg attgaatatg ctggtctagg
    15841 agtagcaatg ggaaatgccg tttctcttgt caaagaagca gcaaatgtca tcacttcatc
    15901 aaacgacgag cacggtgtag ctgaagcaat c
//
LOCUS       DACDYM010000062        15931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDYM010000062 DACDYM010000000
VERSION     DACDYM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.062"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWM59_001986"
     CDS             133..3660
                     /locus_tag="IWM59_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9980011.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWM59_001987"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWM59_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9980012.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWM59_001988"
     CDS             7448..7789
                     /locus_tag="IWM59_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9980013.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWM59_001989"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWM59_001989"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9980014.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWM59_001990"
     CDS             9146..11566
                     /locus_tag="IWM59_001990"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9980015.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWM59_001991"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWM59_001991"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9980016.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWM59_001992"
     CDS             13000..13737
                     /locus_tag="IWM59_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9980017.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWM59_001993"
     CDS             13751..14566
                     /locus_tag="IWM59_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9980018.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWM59_001994"
     CDS             14579..15226
                     /locus_tag="IWM59_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9980019.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSTLAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWM59_001995"
     CDS             15242..15898
                     /locus_tag="IWM59_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9980020.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
BASE COUNT         5341 a         2745 c         3376 g         4469 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcacctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta a
//
LOCUS       DACDYM010000063        15688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDYM010000063 DACDYM010000000
VERSION     DACDYM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.063"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            28..2046
                     /locus_tag="IWM59_001996"
     CDS             28..2046
                     /locus_tag="IWM59_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9980021.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            2473..4887
                     /locus_tag="IWM59_001997"
     CDS             2473..4887
                     /locus_tag="IWM59_001997"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9980022.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5235..6788
                     /locus_tag="IWM59_001998"
     CDS             5235..6788
                     /locus_tag="IWM59_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9980023.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7169..8953)
                     /locus_tag="IWM59_001999"
     CDS             complement(7169..8953)
                     /locus_tag="IWM59_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9980024.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8968..10689)
                     /locus_tag="IWM59_002000"
     CDS             complement(8968..10689)
                     /locus_tag="IWM59_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9980025.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10789..11550
                     /locus_tag="IWM59_002001"
     CDS             10789..11550
                     /locus_tag="IWM59_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9980026.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            12517..12785
                     /locus_tag="IWM59_002002"
                     /pseudo
     CDS             12517..12785
                     /locus_tag="IWM59_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            13457..>15688
                     /locus_tag="IWM59_002003"
     CDS             13457..>15688
                     /locus_tag="IWM59_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980027.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         5188 a         2740 c         3227 g         4533 t
ORIGIN      
        1 agataaattg aagttggagg atagaagatg gaaaacattg caagaaaaga acggagacgt
       61 ttagaacaga ccaaacgttt tcgaaaagca aaacgagggg cagcaatcgt gggaactgcg
      121 atggtaggct gttctgtcgc tgccccatta gtacagccgg ttcctgttca agcggatgaa
      181 acacctgttc agttcggcgc aagaatcaat acagcggcat ttatcacaga aattgctact
      241 tatgctcaac caatcgctca agccaacgat ctatatgcat cagtgatgat cgcacaagca
      301 gttgtagaaa gcggttgggg cagtagtgcg ttatctcaag caccttatta taatcttttt
      361 gggataaaag gcagctatca aggacaaacc gtctacatgg atacgcttga atttttaaat
      421 aatcaatggg taaataaaaa ggagcctttc cgacaatatc cttcttttgc tgaatctttc
      481 aatgacaatg cctatgtact tagaaatact tcatttggca acggttatta ctatgccggt
      541 acttggaaga gcaacacgaa atcctataca gatgcgacag cttgtttgac agggcgttat
      601 gcgactgatc ctggttacgc aggcaaatta aataatatca tcgcaactta cggattgacc
      661 caatatgata caccagctag cggaaacgca ggcggtggag cgactacagg tggcaataat
      721 actggaagtg caggtagtac tggcggttca agcaattctg gtaatacaaa ctcaggttca
      781 gcaacttcga ccacttatac tgtccaatct ggtgattctg tttggggaat ctcaaataaa
      841 ttcggtatca caatggatca attgatccaa tggaacaaca tccaaaacaa tttcatttat
      901 ccaggacaaa aactgacaat caaaggtagc caggaaaatg gctcttcgac gaataattcc
      961 ggtaacaata ccaactcttc aggaaatgct ggtacatcaa acggaggaca aacaactggt
     1021 gcgaaataca ctgttcaatc tggtgattct gtttggaaga tctcaaatga tcacggaatt
     1081 acgatggctc aattgattga atggaacaat atcaagaaca atttcgttta tccaggacaa
     1141 cagctaatcg tatcaaatgg cggttcttct aatgctggag caaatactgg ctcaaatact
     1201 gggaatacaa gtggctctag ttcgtccagc tcaagttctt ctaatacagc ttcaggaacg
     1261 aaatatacag ttaaagccgg agactctgtt tggagcgtct caaataaata cggaattacg
     1321 atggctcagc taatccaatg gaacaatatc caaaataact ttatctaccc aggacaacaa
     1381 ctgactgttt caaacagtgg atcaacaaac agcacgtcag gtaatacagg ttcgtcaacc
     1441 aatacaaata acagcagttc agttaatact ggcagcagtt cgtctcaagg cacttatacc
     1501 gtgaaagcag gtgaatctgt ttggagcgtg gcaaataaaa acggcatcac gatggatcag
     1561 ttaatcgaat ggaataatat caaaaacaac ttcatttatc caggacagca attgatcgtt
     1621 aaaggtggaa cttcggcaaa tacaaataca ggttcaacta cttctgcagc gaaaccaaat
     1681 acaccgaata cgtctgctac gacaagtaca tcatccactg gtaatacgat gtataccgtg
     1741 aaagcgggcg agtcagtttg gagcgtagcg aacaaacatc atatcacgat ggatcagtta
     1801 atcgaatgga ataatatcaa aaataatttc atctatccag gacagcaagt gattgtgaag
     1861 aaaggttcat ctcaatcaac aaaccaacaa tcagttactg gttcgaaaac ctatactgtg
     1921 aaatctggag aatctgtttg gagtgttgct gacagtcatg gaattacaat ggctcaacta
     1981 atcgaatgga acaatattaa gaacaacttt atttatccag gtcaaacgtt gattgtagct
     2041 aaataaaata gctgttcgaa gaaataacga ttgaattcca caaaaatatt tgttactata
     2101 aggctactta aatatgacaa cacttgaaaa ggaagtagta aaaagactga agtttcagag
     2161 agcgcatggc tggtgagaat gtgcacggat gcttttgaac tcgcctttga gttgcccttg
     2221 ctgaataccc gagtaagcaa gcggcggtga cggccgttac aacgttcgag gccttttcac
     2281 taaggtaaaa ttaggtggta ccgcgtagat tacgtcctat aaaaagagca atctttttat
     2341 aggatttttt tatttctttt gtgaagtttg agaaaacaaa gcaaaaggaa ataaaaataa
     2401 acagattctg ccggtttttt cgttatattt ataatagaaa caagtgtttt caatagaaca
     2461 ggaggacagc aaatgagtta caatcataaa gaaattgaga aaaaatggca aaaatactgg
     2521 gcaaaaacga atactttcaa cacccatgat gatcctgaaa agccaaaatt ttacgcactg
     2581 gatatgtttc cctatccatc cggtcaaggg ctacatgtag ggcatcctga aggttatacg
     2641 gcaacggata tcctttcacg tgtgaaaaga agtcaaggat acaacgtgct gcatccaatg
     2701 gggtgggatg cattcggtct gcctgctgaa caatatgcgc tagatacagg aaacgatcca
     2761 gctgagttta cccaaaaaaa tatcgaaaca ttccgacgcc aaatcaattc tttaggattt
     2821 agttatgact ggaatcgcga agtgaataca actgatccgg agtattataa atggacacaa
     2881 tggatcttta cgaagcttta tgaaaaaggt ctagcttatg aagcagaagt agcggtaaac
     2941 tgggtacctg aattaggaac agttatttca aatgaagaag tgatcgatgg gaaaagtgaa
     3001 cgtggtggct atgatgtagt cagaaaacca atgcgccaat ggatgctgaa aatcactgct
     3061 tatgctgatc gcttattgga tgatctagaa ttagttgatt ggccagaaag cattaaagaa
     3121 atgcaaagaa attggatcgg tcgttcagta ggagcaaata ttgaatttaa agtggctggt
     3181 acggataaat cctataccgt atttacgaca cgaccagata ctctattcgg tgcaacttac
     3241 tcggttcttg cacctgaact agatcttgtt agagaaatta cgacccctga acaaaaagct
     3301 gctgtagaag cttatatcga agagacagct aaaaaatcag atctaaagag aacggatctt
     3361 gcaaaagaaa aaacaggagt atttactggt gcttatgcaa tcaatccagt taatggcaag
     3421 gaaatcccaa tctggatcgc cgattatgta ttatcttcat acggcacagg agccatcatg
     3481 gctgttccag cacatgatga acgtgacttt gaattcgctc aaactttcgg actagaaatt
     3541 cttccagtta tcgaaggtgg agatgttgaa aaagctgctt acacagaaga tggtacgcac
     3601 atcaactctg aatttctaga cggcatgaac aaacaagaag caatcgataa aatgaatgaa
     3661 tggctagaag aaaatggcgt agggaaaaaa gaagtcagct atcgcttacg tgactggcta
     3721 ttctcccgtc aacgttactg gggtgaacca atcccaatca tccattggga ggacggaaca
     3781 gtcacagctg tcccagaaga agagctacca ttacggttgc ctaagacaaa aaacatcaag
     3841 ccaagcggta ctggagaatc tccattagca aatatcgagg agtgggtcaa tgtcgttgat
     3901 cctgtaacag gtaaaaaagg ccgtcgcgaa acgaatacaa tgccacaatg ggcaggaagc
     3961 tcatggtatt acctgcgtta tatcgaccct cataacaaaa aagaactagc agattatgaa
     4021 aagttgaagc gttggctacc agttgacatc tacattggtg gagcagaaca tgcggtactt
     4081 catttactat atgcacgatt ctggcataaa ttcttatacg atatcggtgt tgttcctaca
     4141 aaagaaccat tccaaaaact atacaatcaa ggaatgatct taggcgaaaa caacgaaaaa
     4201 atgtcaaaat ctcgtggtaa tgtagtcaat cctgatgatg tggtcgaaac ttacggtgca
     4261 gatacactgc gcatgtacga aatgttcatg gggccattag atgcttcgat tgcttggagc
     4321 gaaaatggct tagaaggaag ccgtaaattc ttggatcgtg tatggcgttt gatcgttgat
     4381 gagaacaata aaatgcgtga ccgtatcact acattgaatg atggcaagct tgataaagtg
     4441 tatcatcaaa cagtgaaaaa agtgacagaa gactatgaaa atcttcattt caatacagca
     4501 atctcccaat tgatggtctt tatcaatgaa gcctataaag tggacgctct gccttatgaa
     4561 tatatcgaag gatttgttca actactcgca ccgattgctc ctcacatggg tgaagaacta
     4621 tgggcaatcc ttggaaatga tggtggtatt tcttacgcac catggccaac atacgatgaa
     4681 gcagcacttg ttgaagacga agtagaagtt gttttccaag taaacggtaa agttcgtgcg
     4741 aaaagcaatg ttccccgaga cttaggaaaa gatgagctag aaaaagcagc attagccaat
     4801 gaaatagtgc aagaatatat tgaaggcaaa acagtcagaa aagttattgc ggtaccaaac
     4861 aagttagtca atatcgttgc taattaattg gctctttgtc aataaggact gatgagttgt
     4921 gcaaaatcaa atctgggcca gaatgaactt cattctgtct cagatttttt gttatttcaa
     4981 attgattaat tgattgatca aaaaggtttt gattttcatg ttctcaaaca cgttcaatgt
     5041 ctttttcttc tgaatggtct tgtaagagat ctctgtaatc atcaaatttt tgtctattaa
     5101 tcttagtatt ttttgatgta taaactacct gaaattgaga gtacatacca aattcataaa
     5161 ttttttatta gtgggactag tgattgtatg gattttgacg cataatgaat gaagtatgag
     5221 aaaaggagag acaaatggat caagaactat ttattatcga agtattatca gctaaacaaa
     5281 aaattatcga gaaagcgaaa aaaagagcga aaagaataat gttcaagctt ttaagcttag
     5341 tatttttctc tggtttgctt tttttcggag ctgtatcgtt cctatatcgc catatgagtg
     5401 cagtagtccg ttcagctgaa gggatgcaaa aattaaataa tttgtatcac ttttcgtggg
     5461 gagttggcgg aataacaatt gttgtgctta ttgtttttct tctacttatt tggctccgtt
     5521 accatcaatt gtcgcgtcat ttcgatcaat ggcttagtca aagagcacaa cagtttttac
     5581 ataataaacc aacttctcaa gatgatgact atctttattt tgagaagact tcgacgagtc
     5641 aagcttttcg gatacaaaaa agtgattgcc agttattgct gagatcactt gatgatttac
     5701 ctatctactt aggcgaaagc aaatcaaaat tcggaatgaa cacattccat atttttttac
     5761 tagaaaacga acccagatgg gaaaaaagcc agtcttcgcc tggtggatac ctgacaacta
     5821 agatgagtac attagtcact ggttttttcc tcattctttt agctggaaca atctattatg
     5881 ggagatacac tcagccaaaa atgtcttctc atataaatgc tgcttctcac atgacaatga
     5941 actcaactaa cgatacgcat atacaaaaga caaatgaagg gttgttagct gatcctaagc
     6001 aaaaggtaaa tttgacaaca caaaagataa atgatctcaa acttgataca gagacgcact
     6061 ctctctatat gacaacagat agtggtgcta attgggtatt tgtgccgatt gattcgcaat
     6121 ggttgcgttt tggcgaatac accctgacaa cggggctgat tcctgaaggt tattggatgg
     6181 acaacacata tgatatttcc gccgatttct catggtttat ctattcttct ggggatgatg
     6241 tacgtctgct gacttcaaga gataatggta aaacatggca aacgtctatt atcacccaat
     6301 cagttcagcc gatacgttat cgaaaagcgg tattttatgg caacgatggt ctatttatct
     6361 gtacatcagc cagtgggatg tctgcagaaa ctttgtacat ttacactact atggatggcg
     6421 gaatcacttg gataaaaagt ggaagtacaa tgatcaatca gccagtacaa aatgtcagct
     6481 tcgttacaac ttctttaggt tttgtttcga cacgtgaaag tttgttttat acaaacaacg
     6541 gaggacgctc attcaaagaa tcagttctgt cgattccgga aaactatcag acagggggga
     6601 tagatctttt tcaatcacca aatgaagtta ctcaaataag cgcgaatacg ttagagacaa
     6661 aattttattt gttgaaaaaa ggaccaatcg atgcagggaa aatgtttgcc tgcctcttcc
     6721 gttcaactga taacggggaa acatggcaat tcgttcagca actatcgcaa gtagaacaag
     6781 gagattaaag aagagagatt gtgagcctga cgttcagcac cgagtcacaa gaaacaaatt
     6841 caaaaaacag cttctcatat ttgttgaata gtgtaactcg tgattcggag ttgacaagag
     6901 caacaagaag cgattttgaa gtatagcttc tcgtatttta caagatcgtg tcttattgcc
     6961 gataggttgt ctcttgaaca ccgtttattc gtaaaaaggg attgtgacaa gtcttttgtc
     7021 actacttacc gaacgaaact gaaggagcta tccaaaaagt atcattagta ttattctttg
     7081 tgtttctagt ggcaccacta gtacatttaa aaaagctatc gatcatggac taatcataca
     7141 tgatcgatag ctttttagat aatgtttgtt acgctatctg aaattgacta ttataaagac
     7201 tggcataaag cgttggtgat tggagtagtt cagaatgtgt tcctttttcg acgatatcgc
     7261 cattcttcat tacaaggatc atgtctgcat tttcaatcgt tgacaaacgg tgtgcaatca
     7321 caaaacttgt cctatctttt gtcacagctt ccattgcttg ctgaatcaat gcttctgttc
     7381 gtgtatctac gctagaagtt gcttcatcta aaataacgac aggaggattc gctagaatga
     7441 ctcgagcaat tgtcaataat tgttgctgtc cttgagataa cgtgccgttt tcgctagaaa
     7501 tgatcgtatc atatccgtct ggcagtgttc gaataaagtg atcacattgt gcgatttcag
     7561 ccgcttcgat aatttgttct cttgtggcat ccattttccc gtaggcgata ttatcagcta
     7621 ccgttccttc aaacagccag gtattttgta aaaccatccc aaaagaacga cgtagttctc
     7681 cacgtgaaag cttggtaata tcaagaccgt cgaattcgat ctttccttta ttcagttcat
     7741 aaaaacgcat cagcagattg actaaagtcg tttttcctgc accagttgga ccaacgatcg
     7801 ctaccgtttg ttttggcttt actgaaaaat cgacttgatt catcaatatt ttatctgatg
     7861 tgtaaccaaa ctgtacattt ttgaaagcta tttttccttt cggttgttct aaaagtcttg
     7921 catctggaag gtctggaatt tcatctgttt catctaatat ctcaaatatc ctatcgattg
     7981 ctgccattgc ggattggatc gagttgatca cataagaagc ggtagaaatt ggttcagaaa
     8041 tctgattgat gtactgaaga taagcttgta agaagccgat cgtcacacca cctgatagaa
     8101 caagcatagc accgaatacc gcacttatga tgaaggcgag ttgattaatg aaacgaatgg
     8161 ccggatagat agcacagttc ataaattgag cacgtttaaa agcgttgtat tgctttttat
     8221 ttacttcgtg tatagcagct tctgcatgag cttgctgatt gaatgttttg accactagat
     8281 taccagctaa gatttcttcc attcgattat ttaaaacacc cagttcatac tgattctgtt
     8341 cagaaatctg tttgtttttg ttcgcaatct tagttgtaac aattgaactg ccaacgatca
     8401 gcaataagac gagcaaagta agcttgcttt cgatataaaa cagcatcaga ccagcagcag
     8461 ctattgttac gacagaagta aaaaattgat tgatacctgt caataacact tgagaaagtt
     8521 gattgagacc ggtagttgtc cgactaatga tgtcacctac ttgatgattg tcaaaaaacg
     8581 acatcggtaa tgttttgaat ttttttgtga tttcttttcg caccctcaat gtgatctttt
     8641 cgcttaacgc tgccattgtc cgttcctgaa taaatgaagt gatgctgctt aagactgaac
     8701 agccgatcag caataagaca gggaacaaca atgcttgttt gatatcactg aaagaaagct
     8761 gtccaatacc tgtttttctt atttctgata agagatcatc gatcccgatt cccatgatca
     8821 agggcatggc tacgactaat aggttcccga ataaactgca tagaaacaat ccgtaaaaat
     8881 atttatgttc aggtttgatc attttccaaa aacgtatcaa agcacgcgtg ttcaacgttt
     8941 ttttcttttc catcgtatcc cttctttcta tattccttgt gattttgcaa actcctgata
     9001 agtttggcaa ttagacagta gttcttcatg agtaccttgt ccaacgattt ttccttcatc
     9061 taaaacaatg atgttatctg cttgttggat tgtactgagg cgttgagcaa caatcacgag
     9121 agttttctcc gacatgtgtt tatgaagggc tgctcttaat gctgcatctg ttttatagtc
     9181 taaagcggag aaactatcat cgaatacata gatctctgct ggtttgatca acgctcgagc
     9241 aatacatatc cgctgtttct ggccccctga ataattagaa ccgccttgag caacaaagct
     9301 gtccaatccg tcaggaaggg tgtccactag ttcttctaat tgagcgatct tgatcgcttt
     9361 agacaactct tctgttgagg catattcatt tcccatctgc aagttgctta aaattgtccc
     9421 gctgaataag aaagccttct gagggacata actgatgtgg gagcggatct gttcttgagt
     9481 catttgagta agaggaatac caccaagggt tacttttcct atcgtagggt cactaagtct
     9541 caagagaagt ttcgccaaac tactttttcc agatccggtc ccgccaacga tcgctgttgt
     9601 ttttccttta ggtattgtga aatcaatatc ttctagaact ggatcagaga tttcagtata
     9661 tttaaatgtg acatgttcaa aagcagctgc atttgtctga tcaaaggttg gttcagctac
     9721 tgttttgatc gtctcttcct caatttcgat ctctgcttgc agtatttctt ctatccgtcg
     9781 taatgaggca aacgatttag ggataatgac caataccata gctgcgatca tcaagtaagc
     9841 aagtgtcagc atagagtatt cgactacagc agtcacgaca ccgatttgta atgctccttc
     9901 tgctacaaga tttccgccaa accacaaaat agaagcataa acgattccca tgagcaaaaa
     9961 agcaattgga gtgacccaag caaatattcg gttcactttg atcatttgat tagcatagtc
    10021 agcaaagctc ttattcgttc gcttttcttc atatgattga ttatcaaagg cacggatcat
    10081 attaatccca gtaaagaatt ctctcaatgt gatggtgatc cgatccattt ttggctggat
    10141 actcaaagat aaaggtgccc ctcgtttcat cagtcgataa acaatccata cataaaaaac
    10201 aacagagatc aatgtaaccc atgttagtaa tagagaataa cgaaacatca tgagaatggt
    10261 gaaaacgcaa ataaccggtg ctggtaagat catttggcaa aataaaacaa tcatttgctg
    10321 gacattatcg acatcattcg tcattcttgt caaaagagat ccagaactaa aacgactgat
    10381 atccttcatt gacagctgtt ggaatttacg gaaatatttc tcccgcgttt ggaatccaaa
    10441 cctagcggaa gccaatgctg aaaagtaact agaggcaact gcagcgattg ttcctagaaa
    10501 cgctacaagc agcatttcaa ctccaatttt ttttataact gaggcttcgc cacttgctat
    10561 acctgtgtcg atcagctttg ccactaattt tggtacacct aatgtaccgg cgatttctaa
    10621 acagatcatc acaaaggtaa ggagagcgag tcctttgttt ttctgtaaga atgtttttaa
    10681 gatagacata ttattcctcc ttttttcctg atacttttcg attctatacc ttcaagtaac
    10741 ttgaaggtca atagaaaaaa gaataaactg aaaaagaggt gagaaaaaat gaaaaaaatg
    10801 tatacgattg gccaagtatc ggaactattt gatttgccaa aatcaacgat ccgctattgg
    10861 gatgaacaag gattaatccg ttcaagtaga caagaagaga atgattatcg attgtttgat
    10921 atcgatgata tttttatgct ttatgatatc gttttttata gaaaattaga tataccgatc
    10981 aaacagatga agaatttata tggaaaaaca ttgacggagt tatatgagac acttgatgaa
    11041 acagaaagaa gaatccataa agaactggtg gtgatgaaac aaaaacaaaa agagataagg
    11101 gagcaaaaaa aacagttgaa attgatgatc gatacaaacg aggaagaatt tcctgtagaa
    11161 gagatccctt ttgactgtat gatctcaacg gagtttgaag atattgtgga aatcaaaaaa
    11221 tttctaccta actattcaag ctttgggatg atgagtatgc cagcttccag ttcacagacg
    11281 atgtatggat tttttatcga ccctagtgaa gtacatttat ttactcaaga cgtgatttgg
    11341 gagaaaaaag atacggctgt ttatcgtcga ttcttattga aatccgagat gaaccatgcg
    11401 gaacgtaata atattttaga aatcagagaa agaatgtatg aaaaaggatg gaaaacagga
    11461 gaagtgatcg gccagtatct attgacgaat acggatgaaa acaatatcag aactgaatat
    11521 tatcatgcat ggatagaaat gaaaaaatag gaaatatgtg tttaagataa ctcgaaaatg
    11581 atggttgttt tagttataaa gaaaaatttt ctggttcgat tacgggttta atgtggggga
    11641 ttattagtcg tggatagata gaattttagt tttttagagg aaatatgtct ttaaaaatgg
    11701 aatggttgca attcttagcg atatatacga tgtaattgtc ctcttaaaaa agtatggaaa
    11761 aataattgta ttgggtgtta tgtataattc atggtgaact gagactaatg agaagaaaaa
    11821 ttcatattgt tatgagtgag attgtatcag atgagaatga taaattaaat aggtgcataa
    11881 aagcattaca cttaaaatac attgttgctt acattattac aatatgttaa taaggcaaaa
    11941 ataaatagat ggattgaata aacatatttt ttaatcaaat agtccctact gtgactttcc
    12001 tcagctatcg gtgatactac cgatagcatt tttttgctta taaaataatt ataagtattt
    12061 agagattttt cttctaacat tcttcaaaca agaaaaatca caaaggttat tgttattttt
    12121 atatattctt tttaagaatg attgtttttt tatagaaatt ttaaaaaata aaacacgtta
    12181 ttgatggata gttagtttgt tatttggtaa tattatagaa gttgactggt agtgatttat
    12241 ttttcgtcgt tttataaaaa tgttattttt tggaaaaatt cacaaatatg taacgaatgt
    12301 ttttctggaa tttgctgtgt taaaaagcag tatgactaat ataaatacta ttgtcattta
    12361 tgcaattttt tatggatagt aattttagag gggaatcaat gaaggaagaa tagaagggaa
    12421 aaatagttgc aaatacgcca gttagcaaaa atagcaatac atcttcaagc tttaaaattc
    12481 gagagaatca tcttggtagt ggaatcaact acaacaaaag gattaaacca attaatagat
    12541 agaattacca atggaaaaat agaaaaggta gttattctat acaaaagttg tttaattcgc
    12601 ttcggttatg aacttagtga aaccctttgc aatatatggt acagaatata gaaattattg
    12661 acattacaga aaatgatgaa gatttaatac agattgtcac tatttgtaga taccttttac
    12721 aaggaaaccg agccaataaa gctaagaaga tgattaagga gttgatggaa gacgatacaa
    12781 gttaagaaag tgaaactacg cccaaataaa gagcaaaaag agtaattgga cgtggaaaat
    12841 gcaagaaatg aaccatcgat tcggtggaca gtttattagt aatgacggtt tgagaacatc
    12901 catgacgaga atgaaggaac atcctaaatg cgtttagttg aaccaagtgc caaataatat
    12961 ggccaaataa gcgatcagtt atcgatgaat cccaagtatt ctaaactata tattcatttt
    13021 gatgctaagc attgatatat ttttttggga ttgatgaagg ttaaccaata cagggactca
    13081 ctgatgaaat tctagagtct gaccttggta tcaacgattt agtgattgtt tctaatggaa
    13141 aagtataaaa gtatataagt acattaacaa aataactatt agggttacgc tccagcttgt
    13201 gcaaggcact ggcaggagga atcgtgaaat cataattatg gttaagccac acggacaact
    13261 aaaagtcata caaagctatc gaatcaatca acagaagaca atcggcaatc attagctttg
    13321 tttacagctt acattgcaac gcttgagttg aaccgaaaat acgagggagg taacgcagcc
    13381 attttgagtt ggggaatctt tttagaaaat tatcgggtac cttgttgttt agtcaaaatt
    13441 ataaggggga gtaatagtga ggaacaaatt tcataaactg ttaagtatgt ttctagttat
    13501 gcttattgtg atcaatcaat ttgggaacat aacggttcta gctgagtctt tagcagaaca
    13561 gccagagcgt tcgctgacga tcgaagttga tcaagaggag gctgctttgg gtgagcagtt
    13621 ccagttgcga gtcagaggaa caccagacct gctaaatgca ttggtcattg ataagagtga
    13681 ggatttgatt gaaggggaag tgaagcaact caatgattac gaacgtctga ttacttattc
    13741 ttcccagcat tcaggtaaat acgaattgac cgcaagaagc ggtgatattc aatcaaatac
    13801 ggtctcaata gaaatcagtg taccagaaga aagcacaagt gagtctttta atgtaccaga
    13861 agaaagcaca agtgaatctt ttaatatacc agaagaaagc ttaagtgaat cttttaatga
    13921 agccagatca tctgagccat ctataacgca ggaatctcag attgaaagac aagaggagct
    13981 cttgaattat gacatcggca tctctgctga gcgccccact ttgccgagca ggcatacaca
    14041 aacgttaaag ggatacattc aaggaggcgc tggcgatatt cttgaagaat atggggagat
    14101 ggtcgtacga gttcctagag catcgattgg cttaattgag ttcattagag tggaggtcgc
    14161 tgatgattca cctttaaagc cactggcatt ttaccaagaa ggcacagacg ttgtttttcc
    14221 atttaccttt gattatgaag gattaaatga agccgctcag tcgtatacat tcagtatcac
    14281 gtttgaagtt gctttctttc aatcaggcgg tactgagcca aataatgact ttcaagtgag
    14341 tcttacttat gctgagcgag cagcaaacac ggaattttca attacgccta ttccttcatc
    14401 aagacccatt ttttcgaaat tatactatgg ctctagagaa atcgacagcc aaggaaatct
    14461 tgggaatctg ctgattgacg ggcaagacgt gaccaacagt aataaattta ttttgcaggt
    14521 aaattatgaa ggatcacgaa aagacggtgt agtggttacc gatgtactac cgcaaggaac
    14581 aactttggca ccgaatccga ctacatggag ttccactgga aatagagaac ctatttcagg
    14641 tggggttcgg atcgcagaag taacgaatcc aggtgaagca aacgttagaa ctcattatgt
    14701 ttccgaggaa tttgtggaca aagtatcttt taataaggaa acgaatacac ttgttgtgga
    14761 ctttggtaat ctagcagagg gtcaatcgta ttttattgaa tatgctcttg aagtgaccga
    14821 tgtcgacttg ttcaatgaag gaagacaaga ggatgggttt tctcggaatc gagcgagtat
    14881 gacctacaat aatcaggtaa tgagccgaga ctaccgcttg agaatccctg aaagtaatct
    14941 tccggcaatc tctttaagaa aaagtgtgga caaaaatgtt atcaatctca atgaaaatga
    15001 actaacgtat actctgcatg tctcgatgtt ttctggaaca agaaatacgc ttgttattga
    15061 agatgttttg gaagaaggca tggaatttgt gtcatttcta tcagatattc cctctttcat
    15121 tgatgccgaa gtcaataatg gaatagttcg tttcagtgcg aataatacga ttggtggtcc
    15181 gacaagtttt accctctctt ttaaggtcaa tgtagaggcg tatgcggtcg gcgatcaaat
    15241 tttaaacttt gcaacgcttg tgaatagtga caatcgattg aatacgagta ctgtaagaac
    15301 aaggaaaatc gatgggcgta ttaaagtaat taaaactgat gagaatggcc aaaggctggc
    15361 aggagcaaaa tttgaaattt tatcaagcga ttcacaagtg atacaagaag gtataacggc
    15421 agaaaacgga gaatttttaa gtgaaccgct tcctgtaggt agctatcaag tccgagaaac
    15481 agaagcaccg gaagggtatc ttttagatgc gacggtacat gatgttttta taaatgaata
    15541 ctccatagaa gaagtgacgt taaccattga aaacatcctt gatacgggtg gtgttgaact
    15601 aattaaacat gctgaaggca gtggtgaagt ccttcaagga gctgtttttg agttacagaa
    15661 ccgagaagga gaaacgctac aaaccggt
//
LOCUS       DACDYM010000064        14757 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDYM010000064 DACDYM010000000
VERSION     DACDYM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.064"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            280..1221
                     /locus_tag="IWM59_002004"
     CDS             280..1221
                     /locus_tag="IWM59_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-beta-xylanase"
                     /protein_id="HAP9980028.1"
                     /translation="METINGITFGFMSQRGDWQDPMSRESLRMMKEELAASHVILPIT
                     VTQAHPQSTKIDWQSDNVLIDAEVKGMIAYAQGIGLKVILKPMVNVADGTWRAHINFF
                     DYDVPCEPTWSQWFDSYREYLNHYAKLAEETGCVMLVIGCELVNSDRREAQWRETISQ
                     IRQHYNGLLTYNCDKYQENNLTWWDAVDVISSSGYYPIDKWEQELDRIEQVVNHYQKP
                     FFFCEVGCPSREGSQYLPNDWSFSGEVSMIAQSRWFEKMFTACDKRNWIQGFGIWDWK
                     ARLYSREMALKDDDYGVYGKPAAAIIREFYKSTKQYI"
     gene            1344..2654
                     /locus_tag="IWM59_002005"
     CDS             1344..2654
                     /locus_tag="IWM59_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9980029.1"
                     /translation="MKKKMLGSMIVLGVAGLVLGACGNNKDSANDKDGGKTETITFIN
                     HKTDWETNGKWDEYMTKFNEKYPDIKVEIQTITDYAGQIKTRMNSEEYGDVLMIPGDI
                     KPEDYGNFFEPLGKKEELSKEYMGLNDRSYDGTSYGLPSQMNATGMVVNQKVFKDAGI
                     DEFPQTPEDFVAALKTIKEKNTDVMPLYTNYAAGWTLSNWDFVRSGASGDKDFTNKMT
                     SDKEPFAAGKVMNTIYKTLYDVSNQGLIEADPTTSDWEQSKVDMANGKIGIMVLGSWA
                     VPQIKDINADTAEDIIFQPFPMTAEDGKQYLPIGADYNYGINVHSKHKEAARKLIDWL
                     VNESDYAEENGGLSAKIGADYPESLKAAQDAGVELIEENPAPAGKESLFSSINDQSEL
                     GLGTTDAEKQRIIDAAVGNSNESFEAIMKDFNTRWSDTIAKVGE"
     gene            2910..3791
                     /locus_tag="IWM59_002006"
     CDS             2910..3791
                     /locus_tag="IWM59_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9980030.1"
                     /translation="MKKSRFSQKTEKRILIFTFTIIPVFLLLMLSYYPLVKMFQYSLT
                     EWNGYSPDSKFVGLDNYKTVLTNPKYFTVFKTSLYYFFATFLQLGVALLFATILSFKV
                     KFANFWKGILFFPYLLNGVAIGFIFLYFFKGDGTLDTVLSALGLESQIRLWLGDRSIN
                     NISLAFTSLWRYTGFNFLIFLGAIQSVNPEVYEAAEIDGANRWDEFRYIIVPSIRKII
                     FLNLILGISGSLSVFDIPYIMTGGSNGTMTFVIQTIDTAFKYNKVGLASAMAVILLVI
                     VLVVTLIQRKFISEKEA"
     gene            3795..4619
                     /locus_tag="IWM59_002007"
     CDS             3795..4619
                     /locus_tag="IWM59_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9980031.1"
                     /translation="MKRQFSIGRILQYLILIIGAVVAVLPILVVFIGSFKSNNEFLST
                     SVLSLPKSLDFSNYKTAFINGQMLTGFKNTLLIFSISMVGKLLLGSMFAYVMSRFDFK
                     LKKPIMALFMMAMLIPGITSQVATFQIIEGIGLFNTIWSVILLNLGTDVISIYVFLQY
                     LDEIPVSLDESAYLDGANYFGIFWRIILPNLKAPIVTMLIISGVGVYNDFYNPFLYMP
                     DRKLKVISTALFAFKGPYGTNWPVILAGVIIVILPILVVFLALQKYIYNGVAGSVK"
     gene            4645..5250
                     /locus_tag="IWM59_002008"
     CDS             4645..5250
                     /locus_tag="IWM59_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980032.1"
                     /translation="MTEKSSKLVVAVELLYVLVKCSLHFWGWLVTGGFIYGWVSSHKK
                     LVFCVDHPEALQEKLCRLTKKLPPTKLCEKVLSVGVFLSLAIFLSGAWLGSTSLGLFF
                     KVTGGLSLLLFLLYNAHWVLGTATYDEMKEAPVAIGYQLLLQTLRPSLLNGFILGTYA
                     FWILLLLVSPLLFFLVAPGGVAYLLKKGSQKFREMEGINHD"
     gene            5243..7465
                     /locus_tag="IWM59_002009"
     CDS             5243..7465
                     /locus_tag="IWM59_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9980033.1"
                     /translation="MISVNPERREFHLKNQNISYIFRVMEELDVLEQLYFGSSIPIYD
                     QYDFLIEREIRPANNQIKQNYTTSLEHIKQEVGLYGTTDFRYPMLRVRYPKGDRISHF
                     TYQHYRIELGKQALSGLPGSFGEAGETLIITLQDRYSPLQVELYYTLFGEESVVTRQM
                     KIINPSETTFELLDCLSFNLDLPNQHYDWLHLDGAWARETQISRNSIAYGVQEVASTR
                     GASSHVHNPFMAVCESNATEEQGKVYGFSLIYSGNFVARLQLDTYDILRIQMGINPFE
                     FAWQLTPKSTFLTPEAVLVYSEAGFNGMSQSFHHFFQEHLISPRWSHKQRPVLINNWE
                     ATYFDFDSEKLLQIAEKAAAIGVDLFVLDDGWFGTRNSDNGSLGNWQVQLAKIPEGIS
                     AFATKINQLGLEFGLWFEPEMISTDTPLYEMHSEWVIGNSEKQISHGRNQFVLDFSNP
                     EVVETIFQQIDCVLACGKITYLKWDMNRYISEPFSNYLADGNQGELFHRYILGVYDLY
                     EKILAKYPDLLIESCAGGGGRFDPGLLYYAPQTWASDDTDGVERLKIQYGASMVYPLA
                     SIGSHLSQIPNHQVGRSPGLTFRNQVAMFGTYGLELDITRMSDVELQQVKAAIATFKE
                     YRELIHEGRFYRLNSPFDNTQCSWMVVSKDLSEALVADYQVLGKPNPAYRRLLLAGLD
                     LEANYQINQLPQLRSGKDLSSIGLIMGGNYVGRAKDYWSRQLPGDYASQLYHLRRVDK
                     "
     gene            7553..8266
                     /locus_tag="IWM59_002010"
     CDS             7553..8266
                     /locus_tag="IWM59_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9980034.1"
                     /translation="MKKYILISSDIRKKILEGVYQANQQIPFEKDLCVEYDASKMTVK
                     KALDMLVSEGLIIKRRGSGTFVKDLATDEIERIAVANQFRGTTALNPGKEVKSKVLEF
                     KVIEADNLVKNKLNMQEDTFVYDIYRVRHIDGKPLVMEKMYMPIDLIPGLKKANIEGS
                     IYEYIEEDLGLKIQSGHRRVSVRKATEIEQQYLELDSGDPVAVAEQIAYFDTGIAFEY
                     SISVHRYDSFSVEFVLTRE"
     gene            8397..9281
                     /locus_tag="IWM59_002011"
     CDS             8397..9281
                     /locus_tag="IWM59_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9980035.1"
                     /translation="MSILAIDIGGTSIKYALWAAEALTEKGSFVTPVTWEQMKMDLKA
                     TLHNLTSCSQTPIEAVSISVPGAVDSDQGIIGGYSAIPYIHKIHFVAELEELLEKPVF
                     VENDANCAGLAEAFYGAAKDAKSSVSFIIGSGIGGAVVLERKLVKGHNLFGGEFGYML
                     AENGQTLSELASPVHAAKRYCEQKGLVSGITGRELFDIADTGDQLAQQEVQQLFRALA
                     IGIYNVCLVIDPEVVCIGGGISMRKDLLAPVKAQLCLLQQHHHSEDQRITVKICQFQQ
                     DANLIGAVANYHLRKEQV"
     gene            9281..10258
                     /locus_tag="IWM59_002012"
     CDS             9281..10258
                     /locus_tag="IWM59_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HAP9980036.1"
                     /translation="MYAIDAKKRWQNYQGSQRKPADFEAFWQAGFQEVDDLGTDYHLE
                     PHPLNSSVAEAFDLTFTGVQGATVHCQLIRPKRLQKKSPLLLQFHGYHSDAGDWSDKV
                     AMAAEGFWVVALDVRGQGGLSEDVGLTTKTSLKGHIIRGVESGPNDLFYRSVFLDIYQ
                     LTRLMVAEPQVDTSQLYTYGASQGGALALICAAVAPEIKESFVCYPFLSDYREAYRLD
                     VENSAYEELAYWFRFKDPLHQREEAFFETLDYIDLQYFAPWIKGKVHWAIGLADTVCP
                     PKTQFAVYNQLSSAADKEMLFYPEYGHEYLPKFGDHMRQCLADVLREKE"
     gene            10265..11653
                     /locus_tag="IWM59_002013"
     CDS             10265..11653
                     /locus_tag="IWM59_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9980037.1"
                     /translation="MLTFPKDFWWGAATSGPQSEGRFQKKHANMFDHWYDIEPEVFYD
                     RVGPDMASNFYNSYREDIALMKQTGLNSLRTSIQWTRLIKDLETNEVDPVGVAFYNNV
                     IDEFLSQGIRPIFNLHHFDLPVELYHKYGGWESKKVVDLFVGFAEQCFALFGDRVKDW
                     VTHNEPMVVVDGEYLFQFHYPKLVDGKKAVQVAYNLNLASAKVMAKFREMGQPQAGGR
                     IGTVLNLTPTYAASDAEADQEAARIAELWNNKMFLDPAIKGEFPVELLELLTKDGVLW
                     QSTADELALIKENTVDFIGVNFYHPNRVKTPDIAPNSVGAWMPDRYYDAYQMPGRRMN
                     IDKGWEIYPKAVYDIAINIRDNYGNLPWFVSENGMGVSREERFMDEKGQILDDYRIEF
                     IKEHLQWLHKGIEEGSNCFGYHLWTPIDCWSWSNAYRNRYGLIANDIHTQIKTIKKSG
                     EWFKTLAENNGF"
     gene            12207..12653
                     /locus_tag="IWM59_002014"
     CDS             12207..12653
                     /locus_tag="IWM59_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9980038.1"
                     /translation="MSDYKKIYETTATNTGGRDGVSYLNDGSFEVKISTPKEMGGSGS
                     GVNPEQLFALGYSACFHGALEIVKGQRKIHQSSQVTHTVKLYKKPDDVDFKLSAEIQV
                     AIQDLDLEEVQKIAEEAHKVCPYSKAVKDSIEVSVRAVLYDADKEK"
     gene            12739..13428
                     /locus_tag="IWM59_002015"
     CDS             12739..13428
                     /locus_tag="IWM59_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9980039.1"
                     /translation="MIDFTKYDHCISTMPLFQGLSLTEIQFIQSDITEQAFSAGQFIY
                     QAEDILESLYLIQEGKVKVYRLGSSGKEQVIRILEQGDFVGELALFEEQHYGCFIEAI
                     SPTKVCSIQKDNFKRILLSHPQIALELLSEMARRLDNTERLTAGITTERAETRLIQYL
                     LEENIKQNTEQIKLETTKKELASYLGMTPETLSRYLTKLTRNNLIKQPIPNIIELTNV
                     TELKNLMDKVR"
     gene            complement(13473..13664)
                     /locus_tag="IWM59_002016"
     CDS             complement(13473..13664)
                     /locus_tag="IWM59_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9980040.1"
                     /translation="MNTLKEDFILAKAGNEEAIEAILKRFSSLIHKKSWRNGKYDQDC
                     YQECMIAVYLAISKFEIKE"
     gene            complement(13740..14126)
                     /locus_tag="IWM59_002017"
     CDS             complement(13740..14126)
                     /locus_tag="IWM59_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9980041.1"
                     /translation="MSENDERVNAQLIRFFEKVIENTANSYFKKQKKISDHEQFDQFP
                     QHFFTENKIKIKQPVTILNVTFLVEDAQLAEIIPLLKEKEQIFLVEKFIFEKTDKEIG
                     ESLGITRQGTTNLKHRLYKKFQELLI"
     gene            complement(14185..14646)
                     /locus_tag="IWM59_002018"
     CDS             complement(14185..14646)
                     /locus_tag="IWM59_002018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980042.1"
                     /translation="MKLEEEKDAIYTQMRMILEERRNLSKDYYELKSRLFTLDSMESH
                     ENSNKNKDFARKSSATISKEAQHQLYISERTNKNKQSIAYSTISLTIASILKGAGRPL
                     SNKEIFKILTNDHGLSISYENLTHNILPRINIDSSINVERAYRGYWQYRLH"
BASE COUNT         4472 a         2485 c         3265 g         4535 t
ORIGIN      
        1 aagcagctct tattagtagt gggatcttat cccgacgacc gtaaaaacca tcatgaatta
       61 gatccatctt accttgccag aaggggatga ttttttgagt gagctgcgtg tacaattttt
      121 tcttatccaa aaaagacctc caattttatt atatttacta tatcatattg tataaatcat
      181 tataatcaaa agtatatatt tttgttgttt ttttcttctt cgtcctttat aatatagaat
      241 tgaaatcgat tacaaaataa aacgtggaga agtgaaaaga tggaaactat caatgggata
      301 acttttgggt ttatgagcca acggggagac tggcaagatc caatgagtcg agaatcatta
      361 cgcatgatga aagaagagtt agctgcaagt catgtgatat taccaattac tgtcacacaa
      421 gctcatcctc agtctaccaa aatcgattgg caaagtgaca acgttttaat agatgcagaa
      481 gtgaaaggga tgatcgctta cgctcaggga atcgggttga aagtaatctt gaaacccatg
      541 gtcaatgtgg cagatggtac gtggcgagcg cacatcaatt tttttgatta cgatgttccc
      601 tgcgagccga cttggagcca atggtttgat tcctatcgtg aatacctgaa tcactatgca
      661 aaactcgctg aagagacagg gtgtgtcatg ttggtaatcg gctgtgaatt ggttaatagt
      721 gatcgacggg aagctcagtg gcgggaaact atttcccaga ttcgacagca ttacaatggt
      781 ctactaactt ataattgtga caagtatcaa gaaaataatc taacttggtg ggatgctgta
      841 gatgtcatct cttcatcagg ctattatccg attgacaaat gggaacaaga attggatcgg
      901 attgaacaag tggtaaatca ctaccaaaaa cctttcttct tttgtgaagt gggttgtcca
      961 agtcgtgaag gaagtcaata tctacccaat gattggagtt tttcagggga agttagcatg
     1021 atagcgcaat cacgatggtt tgaaaagatg tttactgctt gtgacaagag aaactggatt
     1081 caaggctttg gtatttggga ctggaaggcg cggttatatt cgcgagagat ggcgctcaaa
     1141 gacgacgatt acggggttta cggtaaaccg gctgcggcga tcattcgaga attttacaaa
     1201 agtacaaaac aatatatata aaagtatata cttttaaatg ggaatgcagt attatattta
     1261 tgtaatcgtt ttccatttct gacgtaacag gccatgaaca gtttttggta acagcacttt
     1321 attaatttat aggaggaagt aacatgaaga aaaaaatgct aggttcaatg atcgtcttgg
     1381 gagtagcagg tttagttcta ggggcatgtg gaaacaacaa agattcagct aatgacaagg
     1441 atggtgggaa aactgagacc attaccttca tcaaccacaa aaccgactgg gaaaccaatg
     1501 gaaaatggga tgaatacatg acaaaattca atgagaagta cccggatatc aaagtagaaa
     1561 ttcaaacgat tacagactat gctggccaaa ttaaaacccg gatgaatagt gaagaatatg
     1621 gggatgtttt gatgattcca ggagatatta agccagaaga ttatgggaac ttttttgaac
     1681 cacttggtaa aaaggaagag ctcagcaaag agtatatggg tttaaatgat cggtcctatg
     1741 atggcacgag ttatggctta ccgtcacaga tgaacgccac tgggatggtg gtcaatcaaa
     1801 aggtcttcaa agatgctggt attgacgaat ttccacaaac accagaagat tttgtagcag
     1861 cattaaaaac aatcaaagaa aaaaatactg atgtgatgcc gctatatact aactatgcgg
     1921 ctggttggac attatcaaac tgggactttg ttcgcagtgg tgcttctggt gataaagact
     1981 ttaccaataa gatgacttca gacaaagagc catttgcagc gggtaaagtg atgaatacga
     2041 tttataaaac cctatacgat gttagcaatc aagggttgat tgaggctgac ccaactacat
     2101 ctgattggga acaatcaaaa gtagatatgg ctaatggtaa gattgggatc atggttttgg
     2161 gaagttgggc ggtaccacaa atcaaagaca tcaatgctga tactgctgag gacattatct
     2221 tccaaccatt tccaatgact gccgaggatg gtaaacaata tttgccaatt ggagcagatt
     2281 ataactatgg gatcaacgtt catagtaagc acaaagaagc ggcgcgcaag ttgatcgact
     2341 ggttagtcaa tgaatcagac tacgcggaag aaaacggggg cttgtcagca aaaattggag
     2401 cagattatcc agaatcattg aaggctgcac aagatgctgg ggttgagtta attgaagaaa
     2461 atccagcacc agccggaaaa gaatcattgt ttagttcaat caatgaccaa tctgaactag
     2521 gtttgggtac gacagatgct gaaaagcaac gaatcatcga tgcagcagtt ggtaactcta
     2581 acgaaagctt cgaagctatt atgaaagact tcaatacacg ctggtcagat acgatagcaa
     2641 aagtgggaga gtaaaatttt tttagaagtc accctagcag ttaggaaaag acttttgatt
     2701 gatagcaaat aaagttttga ttgtaggcac taacttttgt ctggcaactt gtgccaggca
     2761 aaagcacagt gtcgtaaaga aaacttatct tggtagtaac ttcttttgca ggagtacata
     2821 gcgattgcgg accgtggtcg atatgagggg ttttagaata aggaggagcc aacatgaata
     2881 aacagggaga gtttaccatg aataagacgg tgaaaaaatc ccgttttagc caaaaaactg
     2941 aaaaaagaat cttgatcttt acttttacca ttattccggt atttttatta ttaatgttat
     3001 cttattatcc tttagtgaag atgttccagt acagtttgac agagtggaat ggttattccc
     3061 cagacagtaa atttgtcgga ctagataact acaagacagt cttaaccaat ccaaaatact
     3121 ttaccgtttt taaaaccagc ttgtattact tctttgcaac ttttttgcaa ttgggtgtcg
     3181 ctttattgtt tgcaacgatt ttgtccttta aggtcaaatt tgctaatttt tggaagggga
     3241 ttttgttttt cccttattta ctaaatgggg tagcaattgg atttattttc ttgtacttct
     3301 ttaaaggtga tggaactttg gatacagtgc tatcggcgct aggtttggag agtcaaattc
     3361 ggctatggtt aggagatcgc tccatcaata atatttcttt ggcctttact tccttgtggc
     3421 gttataccgg ctttaacttt ttgatttttt taggagcaat ccagtcggtc aatccagaag
     3481 tttatgaagc agccgaaata gatggggcta atcgttggga tgagtttcgt tatatcatcg
     3541 taccgagtat tcgcaaaatc atcttcctaa atctgatttt gggcataagc ggctccttga
     3601 gtgtctttga tattccatat attatgactg gaggatccaa cggcaccatg acctttgtaa
     3661 tccaaaccat cgatactgcc tttaagtata ataaagttgg tttagcttca gcgatggcag
     3721 ttattttact cgtgattgtc ttagtggtga cgttgattca acggaaattt attagcgaga
     3781 aggaggcata gaagatgaaa agacaatttt ctatcggacg tatacttcaa tatttgattt
     3841 tgattatcgg ggcagtggtt gccgtactac caatcctggt agtttttatt ggctctttca
     3901 aatccaataa tgaatttttg tctaccagtg tcttgtcgct accaaagtca ttggacttta
     3961 gtaattacaa aactgcgttt atcaatggtc agatgttgac aggttttaaa aatacgttgc
     4021 ttattttttc aattagtatg gtaggtaagc ttttgttagg ctcgatgttt gcctatgtta
     4081 tgagccgttt tgattttaaa ctgaaaaagc cgattatggc tttgtttatg atggcaatgt
     4141 tgattcctgg cattactagt caagtagcga cgttccaaat cattgaagga attggcttgt
     4201 tcaacacgat ttggtcagtt attttactaa atttgggaac agatgtgatt tccatctatg
     4261 tttttttgca atacttagat gaaatcccag tctctctaga cgaatcggcc tatttggacg
     4321 gggccaacta ttttggtatc ttttggcgga ttattttacc gaacttaaag gcacctattg
     4381 tcactatgct aatcatcagc ggtgtgggtg tttacaatga tttctataat ccattcttgt
     4441 atatgcccga tcgtaaacta aaagtgatct ccacggcact ctttgcattc aaaggccctt
     4501 atggaaccaa ctggccagtg atcttagccg gggtaattat tgttatttta ccgattttgg
     4561 ttgtcttctt ggctttgcaa aaatacattt acaatggtgt tgctggttct gtgaaataag
     4621 cttaccggaa aaggagggtt actggtgacc gagaagtcca gtaaattagt agttgcagtg
     4681 gaattactct atgtcctagt aaagtgttcc ttgcattttt ggggctggtt agtcactggt
     4741 ggctttattt atggctgggt tagtagccat aaaaagttag ttttttgtgt cgaccatcca
     4801 gaagccttac aagaaaagct ctgtcgacta accaagaagc taccacctac gaaactgtgc
     4861 gaaaaagtgc tatctgtggg cgtatttttg tccttggcga tctttttatc tggtgcgtgg
     4921 ctcggtagca cttcactagg cttgtttttt aaagttactg ggggattatc tttgcttctt
     4981 ttcttactct ataatgcaca ctgggtcttg ggaacagcga cgtatgacga aatgaaggaa
     5041 gctcctgttg ctattgggta tcagctatta cttcagacat tacgacccag tttgctaaat
     5101 ggcttcatct tggggaccta tgccttttgg atcctattat tgctggtcag tcccttgctg
     5161 tttttcttgg tggctcctgg cggagtggcc tatttactga aaaaaggctc gcaaaaattt
     5221 cgtgaaatgg aaggaataaa tcatgattag tgtcaatcct gaaagaagag agtttcacct
     5281 aaaaaatcag aacattagtt atatttttcg ggtaatggaa gagttagacg tcttggaaca
     5341 actctatttt ggaagtagta ttcctattta tgatcaatat gattttttga ttgaaagaga
     5401 aatacgtccc gctaacaacc aaatcaagca aaactacact acttcgttgg aacatatcaa
     5461 acaagaagtg ggcctctatg gtaccactga ttttcgctat cccatgctac gggtacgcta
     5521 tccaaaagga gatcgtatta gtcactttac ctatcaacac tatcggattg aattaggaaa
     5581 acaggcactt tctggcttgc ctggcagttt tggtgaagcg ggggagactt taattatcac
     5641 cttgcaagat cgttattcac ctttgcaagt cgagctttac tataccttgt ttggtgaaga
     5701 atctgtagtg accagacaaa tgaagatcat caatcctagt gagacgacct ttgagttatt
     5761 ggactgtctg agttttaatc tggatcttcc aaatcagcac tatgattggc tacacttgga
     5821 tggagcttgg gcgagagaaa cccagattag tagaaattcc attgcttatg gtgtgcaaga
     5881 agtcgccagt actcgtggag ccagcagtca tgtccacaat ccttttatgg ctgtttgtga
     5941 gtccaatgcg acggaagaac aaggaaaggt ctatggcttt agcttaattt atagtggcaa
     6001 ttttgtggca cgtttgcagc tggatactta tgacattttg cgaattcaaa tgggtatcaa
     6061 tccttttgaa tttgcttggc aattaactcc aaaaagcaca tttttgacac cggaagcagt
     6121 actggtgtat agtgaggccg gttttaatgg tatgagtcaa agcttccatc attttttcca
     6181 agagcacttg atatcacctc gctggtctca taagcaacga cctgttttaa tcaacaattg
     6241 ggaagccact tattttgatt ttgacagtga gaaattatta caaatcgctg aaaaagcagc
     6301 agcaatcggt gtggatctgt ttgtgttgga tgacggctgg tttggtactc ggaatagtga
     6361 taacggctct ttaggtaatt ggcaggtgca gctagccaaa ataccagagg ggatctctgc
     6421 ctttgcgacg aaaatcaatc aattaggttt ggaatttggg ctgtggtttg aacccgaaat
     6481 gatttctacg gatacaccct tgtatgagat gcactctgag tgggtaattg gtaattctga
     6541 gaagcaaatc tctcatggac ggaatcagtt tgtcttggat tttagcaacc cagaagtggt
     6601 ggagacaatt ttccagcaaa tcgattgcgt tcttgcttgt ggcaaaatta catacctgaa
     6661 atgggatatg aatcgttata tttcagaacc attttccaat tacctcgccg atggtaatca
     6721 aggagagttg ttccatcgct acattttagg tgtttatgac ctgtatgaaa aaatcttagc
     6781 aaaatacccc gatttgttga ttgaatcctg cgctggtggc ggtggccgtt ttgatcctgg
     6841 gttactctac tatgcacccc agacttgggc cagtgatgat actgatggag tcgaacgctt
     6901 gaaaattcaa tacggggctt cgatggttta cccattagct agtatcggta gtcatttgtc
     6961 acagattccc aatcatcaag tagggcgttc gccaggactg acttttcgta atcaggtagc
     7021 gatgtttggt acttatggtt tggagttgga tattacgagg atgtcagatg tagagctaca
     7081 acaggtaaaa gcagcgattg ccacctttaa agaataccga gaattgatcc acgaggggcg
     7141 tttttatcgt ctcaatagtc cttttgataa tacccagtgt agttggatgg tagtttcaaa
     7201 agatttgagt gaagctttgg tggcggatta tcaagtgttg ggtaaaccga atccggctta
     7261 tcgtcgttta ttattggccg gtttagattt ggaagctaat tatcaaatca atcaactgcc
     7321 acaattacgt tctggtaagg atctttctag cattggcttg attatgggtg gcaactatgt
     7381 cggtcgagca aaagactatt ggtcccgtca attgccgggg gattatgcgt ctcaactgta
     7441 tcatttacga cgagtagata aataatttca aaaggacagt ggctggctat ttccattcgg
     7501 tagcagatag gataagatat taggataagc catacgtgga ggtaacatga cgatgaaaaa
     7561 atatatcctt atttccagtg atatccgtaa gaagatactg gaaggtgtat atcaagcaaa
     7621 tcaacaaatt cccttcgaaa aagatctttg tgtggaatat gacgctagta agatgacggt
     7681 gaaaaaggcc ttagatatgt tagtgtcaga gggattgatt atcaaacgtc gtggatccgg
     7741 tacttttgtc aaggatttgg cgaccgatga aatagaacga attgcggtgg ctaatcagtt
     7801 tcgtgggacg actgctctca atccaggaaa agaagttaaa agtaaagttt tggagtttaa
     7861 ggtaatagaa gcggataact tggtaaaaaa taaattaaat atgcaggaag atactttcgt
     7921 gtatgatatt tatcgggtgc gtcacattga tggcaagccg ttggttatgg aaaaaatgta
     7981 catgcctatc gatttgattc ctggtttgaa aaaagccaat attgaagggt ccatttatga
     8041 atatattgaa gaagatctcg gtttgaaaat ccaaagtggg catcgacgcg tcagtgtccg
     8101 aaaggcgact gaaatcgaac aacagtatct agaattggat tcgggagatc cggtggcagt
     8161 ggcagagcaa attgcttatt ttgatacagg catcgctttt gagtactcca tttcagttca
     8221 tcgttatgat agtttttcgg tggagtttgt cttaacgaga gaataagtaa gcggaaaaat
     8281 cctctttccg ctttttattt tgattttcaa attttaaaag tatataccta aaaataaata
     8341 actacttaca atgaaaatta gtgtaggtaa gtagactgag aaagtagggg gaattcttga
     8401 gtattttagc aattgatatt gggggtacga gtatcaaata tgctctgtgg gcagcagaag
     8461 ccttgaccga aaaaggttcc tttgtgacac ccgtgacttg ggagcaaatg aagatggacc
     8521 taaaagccac tttgcataat ttaaccagtt gtagtcagac accgattgaa gcggtgtcga
     8581 tcagtgtacc cggagcagtg gacagtgatc aggggattat cgggggctac agtgcgatcc
     8641 cctatattca taaaattcat tttgttgcag aattagagga gttattggaa aaacctgtct
     8701 ttgtggaaaa tgatgccaat tgtgcagggt tagcagaagc cttttatggt gctgccaaag
     8761 atgccaaaag ttcagtttct ttcatcattg gatcgggaat cggtggagcc gtggtgttgg
     8821 agcggaaact agtcaagggg cataacttat ttggaggcga atttggctac atgttggcgg
     8881 aaaatggaca aacgttaagt gagttagcca gccctgttca tgctgcaaag cgctattgcg
     8941 aacagaaagg tttggtatca ggtattacag gtcgagaact atttgatatt gctgacactg
     9001 gagatcaatt ggctcaacaa gaagttcaac aattgttccg agccttggcg attggtattt
     9061 ataatgtttg cttggtcatc gatcctgaag tagtctgtat cggtggtggc atatcaatgc
     9121 gcaaagatct attggctccg gtaaaagccc aattatgttt gttacaacaa catcatcatt
     9181 cagaggacca acgaattact gtgaagattt gtcagtttca acaagatgct aatctgatag
     9241 gagcagtggc aaactatcac ttacgaaagg agcaagttta atgtatgcaa tagatgcaaa
     9301 aaaaaggtgg caaaactatc aagggagtca gcgaaagcct gctgattttg aagctttttg
     9361 gcaggcaggg tttcaagagg tagatgattt ggggacagac tatcatttag agcctcatcc
     9421 cttgaattct tcagtagcag aagcatttga tttaactttt acaggtgttc aaggagccac
     9481 cgtccattgt caactcattc ggcctaaacg actgcaaaaa aagtctcccc tcttgcttca
     9541 atttcatggg tatcactcag atgctgggga ttggtcagac aaggtcgcta tggctgctga
     9601 aggcttttgg gtagtggcac tcgatgttag aggacaaggt ggtttgtcag aagatgtggg
     9661 attgaccacg aaaaccagtc tgaaagggca tataatccgg ggagtggagt ctggaccaaa
     9721 tgatttattt tatcgaagtg tgtttctcga tatttaccag ttgactcggc taatggtagc
     9781 agaaccccaa gtggatacca gccaactgta tacctatggt gcgtcacaag gaggagcttt
     9841 ggcgttgatt tgtgcggctg ttgctccgga aatcaaggaa tcttttgttt gttatccgtt
     9901 cctaagtgat tatcgtgaag cttatcgctt agatgtagaa aactccgcct atgaagagtt
     9961 ggcttattgg tttcgcttta aagacccctt gcaccaacga gaagaagctt tctttgaaac
    10021 attggattac attgatttgc aatactttgc tccttggatc aaagggaagg tgcattgggc
    10081 aattggcctt gcagatacag tctgtccacc gaaaacccag tttgctgtgt acaatcagct
    10141 aagtagcgca gctgacaaag aaatgctatt ttatccagag tatggccatg aatacttgcc
    10201 aaaatttggc gatcatatgc ggcagtgttt agccgacgtt ttgagagaaa aggagtagaa
    10261 tgctatgttg acatttccaa aagatttctg gtggggtgca gccacatctg gtccccaatc
    10321 agaaggccgt tttcaaaaga aacatgccaa tatgtttgat cattggtacg atattgaacc
    10381 tgaagttttt tatgaccgag tggggccaga tatggcttcg aacttttaca atagctaccg
    10441 agaagatatc gcattgatga agcaaacggg attgaattcc cttcgcacct cgattcaatg
    10501 gacccgtctc atcaaggatt tggaaaccaa tgaagtggat ccagtgggtg ttgcttttta
    10561 caataatgtg attgatgagt tcctctctca aggtatccga ccgattttta acctccatca
    10621 ttttgatttg ccagtggagc tttaccataa atatggcggt tgggaatcga agaaagttgt
    10681 cgatttgttt gtgggatttg ctgaacaatg ttttgctttg tttggtgacc gtgtgaaaga
    10741 ctgggtgacc cacaatgagc cgatggtggt agtagatggg gagtacttgt tccaatttca
    10801 ttatccaaaa ttggtggatg gcaagaaagc agtacaagtg gcatacaatt taaatctcgc
    10861 ttccgctaag gtcatggcga agtttcgcga aatgggtcaa ccacaagctg gtggccggat
    10921 tggtaccgtg ttaaacttga cgccaactta cgcagcttca gatgctgaag cagatcaaga
    10981 ggcagcccga attgccgaac tgtggaacaa taaaatgttt ctcgacccag ccatcaaagg
    11041 ggaatttcct gtggaattgc tagaattgct gactaaggat ggggtattgt ggcagtcaac
    11101 tgctgatgag ttagctttga ttaaggaaaa tactgttgat tttatcggag tgaatttcta
    11161 tcatccaaac cgggtcaaga cacctgacat tgctccaaat tcagtgggtg cctggatgcc
    11221 tgatcgttat tatgatgcat atcaaatgcc tggccgtcgc atgaatattg acaaaggttg
    11281 ggaaatctat cccaaagcag tgtatgacat tgccatcaat attcgcgata attatggcaa
    11341 tctaccttgg tttgtttctg aaaatggcat gggggtttct cgggaagaac gctttatgga
    11401 tgaaaaaggg caaattctag acgattaccg catagaattt atcaaagagc atctccaatg
    11461 gttgcataaa gggattgaag aaggttccaa ctgctttggc taccatctat ggactcctat
    11521 cgattgctgg tcctggagca atgcctaccg taaccgctac ggcttaatcg ctaatgatat
    11581 tcacactcag attaagacga ttaagaaatc tggggaatgg tttaagacat tggcggagaa
    11641 taatggtttt taagaagatt taggagggag gagtgaattt gttttcaaaa actcctttgt
    11701 gttggagtaa atggaaaatc tgaacgaaca atagggttta ttgatgaaaa gtcattctag
    11761 tttcttcagt gctttgtcaa atggtgttgt tttgaccaaa tcacacttta ggaaatgctt
    11821 tggcatttcc ttttttgatg taccctgcac gggtagcaac tttgtagctg atggaatctg
    11881 aaggaaacct aaagcaaaaa attatatcat agttcatcca ttttataact aaatcaaata
    11941 tcattcgaat tcaaggaaca gtggatgtgt ttattgcgat aggtggttct tttggaggcg
    12001 caataatagc tttgttaata tttatttacc ttaacaaaaa gaccaataaa catagctaat
    12061 aaagttcaag taaagagata tactgcatcc aacgatattt tttcataaag ttattcttaa
    12121 ctttatgatt cttgatttag atcaatgcca acaccaactt tatttcctaa aataggacca
    12181 taaaagtgaa aaggagtgac ttcaatatgt cagattacaa aaaaatttac gaaacaacag
    12241 cgactaatac cggtggccgt gatggagtga gctatttaaa tgatggatca tttgaagtga
    12301 agatttctac acctaaagaa atgggaggat ctgggagtgg agtaaaccca gaacaattat
    12361 ttgctttagg atacagtgct tgttttcacg gggcattaga aattgttaag ggtcaacgta
    12421 aaattcatca atcttcacaa gttacacata cagtaaaatt atacaaaaag ccagacgatg
    12481 tggactttaa attaagtgct gagattcaag ttgcaattca agatttagat ttagaagaag
    12541 ttcaaaaaat tgctgaagaa gcgcataaag tttgtccata ttctaaagca gtaaaggatt
    12601 ccattgaagt ttcagtacgc gctgttcttt atgatgcaga taaagaaaaa taagtataga
    12661 aaatcgttca ctgatgatat tacatgagtg aacgtttttt tatgctatac taaccacaaa
    12721 aagaggagta gtaagaacat gattgatttc acaaaatatg atcattgtat aagtacaatg
    12781 cctttatttc agggattgtc cctcactgaa attcaattta tccaaagtga tattacagaa
    12841 caagcttttt ctgcaggaca gtttatctat caagcagaag atatacttga atctctttat
    12901 cttattcaag aaggaaaagt aaaagtttat cgcttaggtt cttcaggtaa agaacaggtg
    12961 attcgtattt tagaacaggg tgattttgtg ggcgagctgg cattatttga agaacaacat
    13021 tatggatgct ttatagaagc aatatcacca actaaagtat gttccattca aaaggacaac
    13081 tttaagcgga ttttactatc gcatccccaa atagctctag aacttttatc agaaatggcg
    13141 agacgtcttg ataatacaga acgattaact gcaggaatca cgactgaaag agcagaaaca
    13201 cgcttaattc agtatttgtt agaagaaaat ataaagcaaa atacagagca gataaaattg
    13261 gaaactacga aaaaagaact tgcttcttat ttagggatga ctccagaaac acttagtaga
    13321 tatttaacaa aactcactag gaataatcta ataaagcagc caattcctaa tataatcgag
    13381 ttaacaaatg taactgaatt gaaaaattta atggataagg tgaggtagat aagaagaaag
    13441 ggagaagcaa aatgcatctc cccaaaatga gcttattcct tgatttcaaa ttttgaaatt
    13501 gctagataga ctgcaatcat acattcttga taacaatctt gatcgtattt tccgtttctc
    13561 cacgattttt tgtgtatcaa cgagctgaat cttttaagaa tggcttcaat ggcttcttca
    13621 tttccagctt ttgctaaaat aaaatcttct ttcagtgtat tcatagacca tcttcctttc
    13681 cataaattta aatattattt atgggaggtg gtcgtattaa aagtgaaatc gagtaagttt
    13741 taaataagca attcttggaa ttttttataa agacggtgct ttaggttagt agtcccttgt
    13801 cttgtaatac ctaaagattc tccaatctct ttatctgtct tctcaaaaat aaatttttca
    13861 acaagaaata tctgttcttt ttcttttaat aaaggaataa tctcagctaa ttgagcatct
    13921 tcaactagaa aagttacatt taaaatggta acaggttgct tgattttgat tttattttca
    13981 gtaaagaaat gttgagggaa ttgatcgaat tgttcgtggt cacttatttt tttctgcttt
    14041 ttaaaatagg aatttgcagt attctcaatg accttttcaa aaaaacgaat aagttgggca
    14101 ttcacgcgtt catcattttc tgacatccta ctcacttctt ccaataaaat ttaaaaggag
    14161 aaggaacaga aagcgtgaaa gaagctaatg caatctatac tgccaataac ctctataagc
    14221 tctttctaca ttgatgctac tatcaatgtt tattctggga agaatattat gagttaagtt
    14281 ttcgtaggaa atgcttaatc catgatcatt agttaaaatt ttgaagattt ctttatttga
    14341 tagtgggcgg ccggcaccct ttaaaattga agcaatggtt aaactgatag tcgagtatgc
    14401 aatcgattgt ttattcttat tggttcgttc actaatatat agttgatgtt gtgcctcttt
    14461 agagatggtc gctgatgatt tcctagcaaa atctttattt ttgtttgaat tttcgtgtga
    14521 ttccatacta tctaaggtaa aaagtcgaga ttttaactca taataatctt tactaaggtt
    14581 cctcctttcc tcaagtatca ttctcatttg tgtataaatt gcatcttttt cttcttctag
    14641 tttcatcttc ttctcctttc ataattaaca acgaataaaa agcatgaatg gacacaaaag
    14701 aaatattttt ttagaagaaa taaaaagtga acgttcatat tgaaattgtt ttaaaac
//
LOCUS       DACDYM010000065        14588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDYM010000065 DACDYM010000000
VERSION     DACDYM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.065"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="IWM59_002019"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="IWM59_002019"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9980043.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="IWM59_002020"
     CDS             951..1256
                     /locus_tag="IWM59_002020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9980044.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="IWM59_002021"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="IWM59_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9980045.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="IWM59_002022"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="IWM59_002022"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9980046.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="IWM59_002023"
     CDS             4416..5354
                     /locus_tag="IWM59_002023"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9980047.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="IWM59_002024"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="IWM59_002024"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9980048.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="IWM59_002025"
     CDS             6725..7465
                     /locus_tag="IWM59_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9980049.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="IWM59_002026"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="IWM59_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9980050.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="IWM59_002027"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="IWM59_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9980051.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="IWM59_002028"
     CDS             10608..11258
                     /locus_tag="IWM59_002028"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9980052.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="IWM59_002029"
     CDS             11259..11897
                     /locus_tag="IWM59_002029"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9980053.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="IWM59_002030"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="IWM59_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9980054.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="IWM59_002031"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="IWM59_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9980055.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13674..>14588
                     /locus_tag="IWM59_002032"
     CDS             13674..>14588
                     /locus_tag="IWM59_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980056.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTP"
BASE COUNT         4896 a         2704 c         3175 g         3813 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    13441 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    13501 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    13561 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    13621 gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga agagtgaaaa
    13681 agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta gcatcaccga
    13741 tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa aagattgcag
    13801 acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa agccaagttt
    13861 ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg cgtcaagaat
    13921 ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa cgtgaagata
    13981 caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac tacatcgatg
    14041 ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca atgacaacga
    14101 tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa gctgtagaag
    14161 acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa gctgctttgg
    14221 aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg aaaacttcat
    14281 tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt caaaaagcag
    14341 aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa caagctcgtc
    14401 aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag gctgaagcac
    14461 aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca tcagctacac
    14521 aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa
    14581 caacacct
//
LOCUS       DACDYM010000066        14362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDYM010000066 DACDYM010000000
VERSION     DACDYM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.066"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            201..2672
                     /locus_tag="IWM59_002033"
     CDS             201..2672
                     /locus_tag="IWM59_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9980057.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2775..4511)
                     /locus_tag="IWM59_002034"
     CDS             complement(2775..4511)
                     /locus_tag="IWM59_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9980058.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4508..5212)
                     /locus_tag="IWM59_002035"
     CDS             complement(4508..5212)
                     /locus_tag="IWM59_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9980059.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5343..5846
                     /locus_tag="IWM59_002036"
     CDS             5343..5846
                     /locus_tag="IWM59_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9980060.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5806..6144
                     /locus_tag="IWM59_002037"
     CDS             5806..6144
                     /locus_tag="IWM59_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9980061.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6165..7583
                     /gene="ffh"
                     /locus_tag="IWM59_002038"
     CDS             6165..7583
                     /gene="ffh"
                     /locus_tag="IWM59_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9980062.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7695..8273
                     /locus_tag="IWM59_002039"
     CDS             7695..8273
                     /locus_tag="IWM59_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9980063.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8277..8798
                     /locus_tag="IWM59_002040"
     CDS             8277..8798
                     /locus_tag="IWM59_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9980064.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8877..9431)
                     /locus_tag="IWM59_002041"
     CDS             complement(8877..9431)
                     /locus_tag="IWM59_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9980065.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9684..9959
                     /gene="rpsP"
                     /locus_tag="IWM59_002042"
     CDS             9684..9959
                     /gene="rpsP"
                     /locus_tag="IWM59_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9980066.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9971..10222
                     /locus_tag="IWM59_002043"
     CDS             9971..10222
                     /locus_tag="IWM59_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9980067.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10347..11138
                     /locus_tag="IWM59_002044"
     CDS             10347..11138
                     /locus_tag="IWM59_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980068.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11308..11829
                     /gene="rimM"
                     /locus_tag="IWM59_002045"
     CDS             11308..11829
                     /gene="rimM"
                     /locus_tag="IWM59_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9980069.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11819..12580
                     /gene="trmD"
                     /locus_tag="IWM59_002046"
     CDS             11819..12580
                     /gene="trmD"
                     /locus_tag="IWM59_002046"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9980070.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12716..13063
                     /gene="rplS"
                     /locus_tag="IWM59_002047"
     CDS             12716..13063
                     /gene="rplS"
                     /locus_tag="IWM59_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9980071.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13321..13614
                     /locus_tag="IWM59_002048"
     CDS             13321..13614
                     /locus_tag="IWM59_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9980072.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4825 a         2581 c         2914 g         4042 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaaccctt tttttttgct atatgacagg aatgaaaggt gtttgcaagc ttaatgtgag
      121 gaacactttc catatctgtt ctatactgga taggagaagt ttggaaatat ccagaaaacg
      181 aaatcaatcg aggtagccac atgaagaata aatggataaa tcgaagtaaa aaaaagaaag
      241 taacacaaag atcatcagat aaagtcatcc gtctttttcc atcgtataaa gaaggattaa
      301 cggcagatca agtcaaggaa cgaatagaaa aaggtgcagc aaataattca gtcgatccca
      361 cattcaaaac aaatcagcaa atcgtattgg aaaacatatt tacgtatttc aatttgatct
      421 ttcttatctt agccattttg ctttgtttag tagaatccta taagaacttg acttttttac
      481 cagttatcat agccaataca ggaatcgcca tttttcagga aattcgatcc aaaaagatct
      541 tggacgaact aaatgtttta aatacagcaa aagtcaatgt catacgagac gggatagaac
      601 aaacaataga tatagaagac cttgtaatag atgacattgt tattttagag actggatatc
      661 aaattccagc agatgcagag gtcgtggaag ggaaactgca agttaatgag gctttattga
      721 caggcgaagc agatgaaata accaaagaaa taggtgatcc attaatgtcc ggcagtttca
      781 ttgtttccgg aaaagcttac gcccgattag acaaagttgg agcagattcg tatatttccc
      841 agctgacagt caaagccaag acaatgggag aaggagaaca gtcggagatg attgcttctc
      901 ttaatcaatt gatcaaatgg attgggatca tcattattcc tatcggtatc ctgctttttt
      961 ctcaaagtta cttttttaat ggaaacacga tcaaagaaag tgtcgttgca atggaagcag
     1021 cattgatcgg tatgatacct gaaggactgt acttgctgac gacgattgca ttagcattaa
     1081 gtgcgactaa actagcaaag caaagagttc ttttacacaa tatgaaaagc attgagacat
     1141 tggcaagggt aaatgtcctt tgtgtagata aaacaggaac gatcacagaa aataaaatgt
     1201 ctgttcaaaa aatgatcgtc tctaaaaaac aagaaataat cagtcagtca gatcaattgg
     1261 aagcatgcat tgctgactat gcaaaagcga tggcaggaga caatgcaaca atggaagctg
     1321 ttaaagcata ttttacaaaa accactgatc aatcctatca ccatgttatt cctttttctt
     1381 ctgtccaaaa attcagcagt gtagcgttgg cagacaaggt gtatgtttta ggagcgcccg
     1441 aaatggtttt gcgcgatcaa ttgccggaat atgcggaaga gtttgtttct ttcgctgaaa
     1501 aaggctatcg cgtgttggtc tttggtgtgt acgatgggat tcttgaagaa ccagtattga
     1561 cagaagcagt cacaccatta ggctatattc tgattgctaa tcctatcaga aaagaagcaa
     1621 gagccacatt tgattacttc aaaaaacagc aagtagccat caaagtgatt tctggagata
     1681 atccgctcac cgtatccaat gtagctgtcc aagcagggat ttcacatgct gatcagtacg
     1741 ttgatgcatc taaattatca gaagaagaat atgaagcagc tgtcgaaaaa tatacagttt
     1801 ttggacgcgt caagccagaa caaaagaaaa tcttcgttca attgataaag cgtaacaata
     1861 cagtagctat gacaggtgat ggcgtgaatg atattctagc catgaaagaa gcagactgca
     1921 gtatagcgat ggcttccgga aatgaagccg ccatgcaggc ctcacaagtg gtcttacttg
     1981 attctgactt ttcacgcatg ccagaagttg ttgcagaagg acggcgcgtt gtgaacaata
     2041 ttgagcgatc agccagcttg tttttagtga agaatatttt ctcctttttg ctctcactgt
     2101 tttcagtgat ctttgctctg acctatcctt tagaaccatc acaaatcaca ttgattagtt
     2161 tgtttactat cggacttcca tctttcttgc ttgctctaga agaaaacaaa aagcgaatca
     2221 gagggaaatt catcatgaat gtgatggaaa aagctgtgcc aggtggacta acagatatga
     2281 tcgtagtcgg tgcgttggtg atctgcgggg tgactttgaa tctaaataaa acagatgttt
     2341 ctacagcatc taccatgctt ttgattgcag tagggttcct cgttctctat aaaatctgtt
     2401 caccgctgaa taaattcaga agtcagatca ttcttttctg tgctagcggt aacttttttt
     2461 cggtcatctt tttgcataag ctgttttcaa tcaccggtat ctcagctgta tcacttttgc
     2521 tgttgagcat tctatttttc tcggcagatt cgatttttag gcatttgacc acattggtag
     2581 aatatttgtt tgaacgtaaa aatgaaaaag agcaaaagcc gtttagtcgc gtagctaaaa
     2641 aatttctatc gatatttaaa cgagaaaatt gacgaaaagt atctcaaaaa agagtaaaaa
     2701 agaacaacca ctcgtgtatc tttcgaagca ggatgagtca tccaacttta aaacgcacga
     2761 gcagttgctc ttttttacat tttcggaatg gtaacggtga aagtcgaacc tgttccaaga
     2821 tgactgtcta cagtaatcgt accattaagg agttgcacgt agtcttttac aatcgctaac
     2881 cctaatccgg tcccgcctga atgtcggctt cgcgccttgt ctacgcggta aaaacgctca
     2941 aagatccgtt cctgatcctc aatgtcgata cctataccaa agtcttggac agaaaacgat
     3001 agatcatctg ttgcctgata gcggataata atctcgctgt cgctttttga atattggaca
     3061 gcattttcaa tcagattttt gataattgga taaaggatat ctgttttagt agtaaatggt
     3121 tcgttctttc caatcaatcg aatcgtcaac cgtttctttt cgatgattgg ctgataattt
     3181 tgaacgattt gctggaaata aggttctgta taaattgtct ggatctcgta agaattctcg
     3241 ccatcttttg acagctggat gatttcccga atcagtttat ctaaccggat cgcatctttt
     3301 tgcatgatct caagaaaaga agtcagagtt tgcgggtcat cttttgcacc atccagtaaa
     3361 gtctcggtaa aaccaatcag tgaagtcaca ggagttttta attcgtgaga gacgttgcca
     3421 acgaaatcct tttgtagctt ttctaaacgt cgcactttcg ttaagtcata cgccacccct
     3481 agaatctgtc cggtaccttc tgaattagta aagtagcgca aactcaagtc taatgtctgc
     3541 cgaccttctg taagggtgat ctcctgatgg atcagcggat gctcaggagt cacttgatga
     3601 atcagctgaa tgagttttgg ctcttgaatg acttctgtgt accgctgttc aggttgatag
     3661 cttaccacgt ttaaatgtag ctgcatcttt ggattaagca gtagtaaacg agaatcagca
     3721 tcatcaatca ggaatacgcc aatcatcagt tcgttcagta acgtatataa ttgctcttct
     3781 gtggtagtac acgcccgata catcttactc atttgttcgc tcaacgcatt aatcgtctga
     3841 tacaattctt cccattgatc gggtgtttgc atgattactt ccgcctgatt aggattttgc
     3901 accatttttt ttagtacggg cagcacggtt ttcaatggct ggtttttttg atagatcaga
     3961 taatagatca ttcctgcaag aacaaggaag aaagacagaa agaagaaaaa gacccaacgg
     4021 cgaaaactct cagttctcgg taaaaaaacg cttgtcggtt ctgcgatacg taaaatggct
     4081 tctaactggc cacttttttt gactggtaat gcgacatata aaagatcctc attcaaagta
     4141 gtacttttgc gcaaagcgct acctaggttc ccaccattca atacggcttt gatttcggga
     4201 cgggagtttc gctgttcatt caatgtttca ttgtttgtgt caaataagat ctttccagta
     4261 gcatccatta aagtaatccg ttcgttcgac tgatggacga attcttccaa agcagttttg
     4321 ttttgcgctg cttggaggtt ttcgacatct aattgccgga tcagcagttg tgcttttttt
     4381 gtcaggtagc tttcttgctg ttccacgatc tgctgttgaa aataatggga aatcaattgc
     4441 cagccaccaa aaaacaaact aactaaaaga ataagaaaga agagacgttc ataaattttt
     4501 ttccatttca tcgtttgggt tcctgaaact tatagccaaa tcctctaata gtcaccaagt
     4561 actttgggtg ttttggatct cgctcgattt tttctcgcaa atgactaacg tgtacatcta
     4621 cgattcggct ttgtcctgcg aagtcaaaat tccaaatgcg gtctagcaat gtgtctcggt
     4681 cgatcacacg gtcttttcgt ttcatgaaat aaacaagtaa ttcaaattct ttcggtgtta
     4741 gttcaatcag atggtcgtct acggtcactt gataattggt caaatcagca gtgatttgtc
     4801 caatcgatag atattcaggc tcagcttcat cttttgattg cttacgaggt tcaattcgtc
     4861 tgaatattgc tttcatacgg gctaatactt cgcgaggact aaatggtttg gtcaaatagt
     4921 cgtcagcacc tatttctagg ccaatgatcc gatccacttg gtcatcttta gccgtcagca
     4981 tcaaaatcgg tgtttcattt ttttcttgac gcagccgctg agtgatctcc atgccatcca
     5041 tcgttggaag catcacatct aaaatgatga aatcaaatga ttgttctaat gctaattcta
     5101 atccttcgcc tccatctgtg gcactggtta ccttgtagcc ttctttttcc aaattgaacg
     5161 tcaagagcgt tacaatagaa ggttcatcat caacaactag tatttttttc attcaaatct
     5221 cctttaaata ggtttcattc tgtttatatt ttagcataga atcagtacaa agggctattt
     5281 tacaaaagaa ataaatggta taataggaag gcactttgct gaatagaaag gaagagcagt
     5341 caatgattgg aatcagtgca tgtttaggcg gtctggcctg tcgctatgat ggacagaaaa
     5401 aagaagtacc agaattgatg gaactagttg aaactggaga agctgtcatg gtctgtccag
     5461 aagtgatcgg cggattacca attcctagag atcccgctga aatcattggt ggaaatggat
     5521 tcgatgtgtg gaaagatcaa gcaaaagtat ggacgacaag cggcgaagat gtaacggaag
     5581 cgtacaaaaa tggcgcgatc aaagcctatc aaaaattgca ggaaaaacac attgatctag
     5641 tcatcatgaa ggaaaaaagc ccttcatgcg gaacccattc gatttatgac ggaagttttt
     5701 ccggaataaa aaaaaatggt gccggagtgg ctactgctta ttttattcag caaaaaatga
     5761 ctgtgctctc tgatgaagaa tggctgaaaa ctagaggtga gcagaatgga aatcgaaaag
     5821 accaatcgaa tgaacgcgct ctttgaattt tattcgacct tattgacaga aaaacaaatg
     5881 aactacatgg aattatatta tgcagatgat ttttcattag gtgagatcgc ggaagaatac
     5941 gaagtaagcc gacaagcagt atatgacaat atcaagcgga caagcaagat cttggaggat
     6001 tatgaaaaaa aactccatct tttttctaat tatattgttc gagaacaggt attggaaaat
     6061 atgactgcat atattacaga taagtacccg gaagataaaa aactacttga atatgtacag
     6121 caaattcaag caatcgaaga ataaataaaa ggaatgaaga aaatatggca tttgaaagct
     6181 taactgaacg tctccagcag gcgatgggaa aaataagaaa aaaaggaaaa gtatcagaag
     6241 ctgacgtaaa agaaatgatg cgggaaatcc gccttgcctt actagaagcc gatgttaact
     6301 tgcaagtagt caaagatttt actaagcgcg tacgcgaacg tgcggtaggg gcagaagtgc
     6361 tggaaagttt atcacctgca caacaaatcg taaaaatcgt tgatgaagaa ttgacgatca
     6421 cacttggttc tgaaacagca gagttaaata aatcaccaaa aataccaact gttatcatga
     6481 tggctggtct tcaaggggca ggtaaaacga cctttactgg taaattagcc aattatttaa
     6541 agaaaaatga aaatgctcgt ccgctcctaa tcgcagggga cgtatatcga ccagctgcga
     6601 tcgaccaatt gaaagtatta ggtcaacagc tagacgttcc tgtatttgat atgggaactg
     6661 aagtcagccc ggtagagatc gtccgtcaag ggatggagtt agcaaaagaa aagaaaaacg
     6721 actatgtatt gatcgatacg gcaggacgtc tgcatatcga tgaaacattg atggatgagt
     6781 tgaaacaaat caaagaattg acgcaaccaa atgagatttt gcttgttgtc gatgccatga
     6841 ctggtcaaga tgccgtcaac gtagctgata gcttcaacca gcaattaggt attacagggg
     6901 tcgtcatcac taaacttgat ggggatactc gaggcggggc agcactatcg atccgctcag
     6961 tgacaggcgc accaatcaaa ttcatcggtt ctggtgaaaa attgactgac ttagaagtat
     7021 tccacccaga tcgtatggct agccgtatcc ttggtatggg ggatatgctg acattgattg
     7081 aaaaagcgca gcaagactac gacgagaaaa aagcggaaga acttgcacag aaaatgcggg
     7141 aaaattcatt cgatttcaat gactttattg aacagcttga tcaagtgatg ggtatgggcc
     7201 cactggaaga tctaatcaaa atgatcccag gaatgaatca agtccctgga atcgaaaacg
     7261 tcaaggtaga tccaaaagat gttgaacgca aaaaagcgat ggtttattct atgacgccag
     7321 cagaaagagc aaatcctgat ctgttgaacc caagccgtcg tcgccgaatt gctgccggtt
     7381 ctggaaacag tgttgttgaa gtgaatcgaa tgatcaaaca attcaaggaa tcaagaaaaa
     7441 tgatgcagca aatgtccaaa ggggatatga acatcccagg tatggaccaa atgtttggtt
     7501 caggcgtcaa aggaaaacta ggcaaaatgg cgatgaatcg aatgatcaag aaaaataaga
     7561 agaaaaagaa aaaacggaaa taagatgaaa aaaacagctt cggtacagac tgaaaatgtc
     7621 tactgccgga gcttttctat cagcaatttt tattctttac taatacaatt actctaaacg
     7681 aagaggtggc acagatgaga tgtccgtgga gtgaacaaac agaaagtatg cggcaatatc
     7741 atgatactgt ctggggtgtt cctgaatatg atgaccaaaa gctgttccga aaattgatgc
     7801 ttgatattaa tcaagccgga ttgagctggc agacgatttt aaataaaagt gaagcattcg
     7861 atgaagcata tgatcgattc gatatcaaaa aagtcgcaaa ttatggagaa acaaaaatca
     7921 gtgaactaat ggaaaacaaa ggaattattc gtaaccgaag aaaaatcgaa gcagccgtcc
     7981 aaaacgccaa agcagtcttg aaaattcaag aagaaatcgg cagtttttca gagtatcttt
     8041 ggtcgttttc taatggaaaa atcgttgatt cttcttatca ttcacaagaa gaagtcccaa
     8101 caacaagtcc tctttcagat gaattagcaa aagatctaaa gaaaagagga tttaaattta
     8161 tcggaagcac aacaatctat gcttttttag aagctgtcgg gatcatcaat gatcaccttg
     8221 acagttgttt ccgcaaagaa gaggtcgtac gtatcggcag aaagggcgaa taaagcatgc
     8281 gaaaaatcat attttatggt gctataagtt tagatggcta tttggcaacg aaaacggatg
     8341 atcttcaatg gctttttgac actccaacag gtaagcaaac tacttatgaa gctttttatg
     8401 aaacaatcga tacgaccatc atgggcagaa aaacttatca ggaagcaaaa aaatatttgg
     8461 agaccgaagc aatttatcca gagaaaaaga atttcgtttt ttccacccac gcacaattac
     8521 aattgccaga tgctgaggtc gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa
     8581 tgacagaagg aaaagatatc tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg
     8641 aacatcaaat gattgatgaa tggtatatcc agattacccc tgtattatta ggggacggga
     8701 ttcctttatt ccaaaaagga acatatgaag cgcgttttga aatgctagac acgacacgat
     8761 tcggtgaatt tatcgaattg cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc
     8821 aactatatgc agaaatgtcc tgtacatagt tgcctttttt attaccgtta tgctttttac
     8881 tctgttaatt tgttagaaaa atctagatac gcatctaaaa tcgtttggaa gaaacttaat
     8941 gttccttcaa cgatattacc attttcatca attagattcg caacgtttcc gatatatgct
     9001 tccggttgct gcaaggttgg cacattcaaa aagacaagtg attggcgtag atggtggttt
     9061 gcgccaaagc cgccgatccc acctggtgat gcagtaacga ctaaacctgg tttatgatcc
     9121 caaacactgt gtccataagg tcttgagcct acatctaaag catttttcaa tactgctggc
     9181 acagaacggt tgtattcagg agtaacgaaa acgacaccgt ctagtcccct cacttcctca
     9241 cgaaaacgag tccattcagc aggagcctgt tctggagttt cgagatcctc attgtaaaat
     9301 ggcagatcat cgatttttac aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa
     9361 gcttctgcta cttttttgtt gtaagaatct tttcttaaac taccaataaa aaatccaatt
     9421 ttttttgtca tacgattttt cctccttcaa actatctata ataatcttac aaaagataag
     9481 taactggagc aaataaaaac acttactgac aaaagctaca tagaagatag attacttatt
     9541 tttaacaaaa aagaagtcta aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc
     9601 tgtaaagaaa aaaagcttta cagggtaaaa aaaaactgtt acactacatc ttgtgaataa
     9661 aacaatggag gtggaaataa ataatggcag ttaaaatccg tttaaaacgt atgggttcta
     9721 aaaagagtcc gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt
     9781 tcatcgaaac tgtaggtact tacaatcctt tgaaagaccc tgcagaagta gttttaaaag
     9841 aagacttagt tcttgactgg ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca
     9901 tcctttcaaa agaaggcgtt atgcaaaaac accacgaagc taaattctca aagaaataag
     9961 gtgaccggat atgaaagatt taagcgactt agtcttaaca atcgttcgtc cgctagtcac
    10021 ttatcctgat caagtcaagt tagacgtcgt agagtcgaaa gacttttacg agtacaatct
    10081 aacagtggct cctgaagata ttggccgtat cattggaaaa caagggcgtg ttgctaaagc
    10141 aatccgcaca atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat
    10201 catcgatgat aaggaaaagt aaataaacgt gctgtactcg aattttcgag tgcagctttt
    10261 ttgttttttt tattaattga agaaatgatt atttggcaaa acaagcaaga aagcgtatcc
    10321 ttataggagg agggagtgaa cagccaatga gaaagaaaat gttatttatt ccatttgctc
    10381 tattgttaag cgcttgtagt tcggttactc aagaatcaga caatacacat ctatcaagca
    10441 gtatatctga aactgttacc agtccagaaa aaagtagtac caaaacggct tccaccactg
    10501 aacaaacaat ggctacctca aataatgaga aaaagactgc tttagaccag ttgaaagaac
    10561 aacagccaaa tgttcccatg cctctagatg taccagtctc ttcaggctat ctcaatatcg
    10621 cagcgacaca tacaaagcaa ggttattcta ttttatatta cagaacagat cgtcccctcg
    10681 ggttgaatgc tgacgaattg aatcaagaaa cgcctattgc aacctatcta tatcaatatg
    10741 ggtttgcttc tagtcaagaa acaatccaag ttttgcagcc atttgagatc gacacaaatg
    10801 gtcagcaagt ggacttaggt tctcgaatca cagggtatca gcaaggtgca gcaggttcaa
    10861 gttttcttga atggcaagaa ggaaactggc gtattcgaat ccgtgggaat aatatagaag
    10921 gccaagatcc cttactatta gctaaagaaa tcgtagctta tttggaagaa aacagtctac
    10981 cagcacctga acaatttggt aaaattactg ttgatatggg tgatacgacg aatcgagccg
    11041 tagaagtaag ctggcaagaa ccgaaaaatg cctatacgat cactcatcaa gatcctatgt
    11101 ctgcgttgaa gatggcagtt tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc
    11161 catcatttaa ctccaagtca caggaagtaa tcaaaaatag cacgccgcat ttttgcataa
    11221 ttttggctta ataccttaaa agaaaactag aattgcctct tcacacatgc taaaataaaa
    11281 cagacaaaaa agaaatgggg atataagttg actgaatact tgaatgtagg aaaaatcgta
    11341 aacacacaag gtatcaaagg cgaagtccgt gttatctcaa caacggattt cccagaagaa
    11401 cgttacaaaa aaggaacggt gctaacacta ttccaagacg gaaaagcacc tgtggaatta
    11461 acagtcaaaa gtcatcgcaa acataaaaat tttgacttat tgagttttga aggacatcct
    11521 tctatcaatg atgtggaaaa atatcgggat ggcatattaa aagtgtcaaa agacaactct
    11581 gtagatctag ctgaaaatga attctattac catgagatca tcggtctgca tgtattagaa
    11641 gatggaactg agttaggtaa agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg
    11701 gtcgttcaac gtccgaaaaa agctgatata ttgcttccat atatcgcttc tgtcgtcaaa
    11761 gaagtcgatt tagaagctgg agtggtccgt gtagaaattc cagaaggact gattgacgat
    11821 gaagattgat gtattgactt tatttccaag aatgtttgaa ggtcctatgg gggaatccat
    11881 catcggtaag gctgtagata aaggattatt agatatcaat atctctaact tccgcgatta
    11941 ttcagataac aaacatcaga cagtagatga ttatccatac ggcggtggag caggtatgct
    12001 gctcaaagtc cagcccatct atgataacat ccaagccatt gaaaaagctg caccggatgt
    12061 caaaaaaaga gtaattcttc ttgacccagc tggtaaacga ttcgatcaga aaatggcaga
    12121 agaattttca caagaagaac acttagtatt catttgtggt cattatgagg gatatgatga
    12181 acgtatccgc acattagtga cagatgaagt ttccttagga gattatgtcc ttactggggg
    12241 agaattaggt gcgatggtca tgatcgatgc aacagttcgg cttttgccag atgtgttagg
    12301 gaatcagact tcagctcaaa cggattctta ttcaaccggt ttattggaac atccccaata
    12361 tactcgcccg gcagaatata aagggatgaa agtaccagaa gtattgacaa atggaaatca
    12421 taaattgatt gaagaatggc aattaaaaga atcactaaga aggacctatc aacgtcggcc
    12481 tgatcttttg gaaacacttg aatggacacc tcaaatgacg aaatttttag aagaaatcaa
    12541 gaaagaagaa caagaaaaaa cagcagatga agccaagtga ctttttctaa aatcttcttt
    12601 acagtaggtt gataatatgt tagtatttta tggtgagtgc aaaagcactt aactattacg
    12661 atattccgct gtgagaaaag ctcataagaa tatttggaat aaggagaaga aaaccatgaa
    12721 tccattaatc gaagaattaa caaaagaaca attacgttct gacatcccag cgttccgccc
    12781 tggtgacact gtgcgtgttc acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt
    12841 atttgaaggt gttgttatca aacgccgcgg tgctggaatc agcgaaactt atacagtacg
    12901 taaagtttct aacggagtgg gcgttgaacg tacattccca ttgcatacac cacgtgttgc
    12961 aaaaatcgaa gtagttcgct acggtaaagt acgtcgtgca aaattgtact acctacgtgc
    13021 attacacgga aaagctgctc gtatcaaaga aatccgtcgt taatcattgt tatcagactg
    13081 agaaagaacc cttggtattc aagggttctt ttttcttagc atgtaatttg aacaccctga
    13141 tccagttttt tctttaatta ttggtagggg agaataatgg attagaataa atctacctat
    13201 aagtagtagt aaaaaaaaga gcactatgat aatatctttc ttaagagtaa ccgctttaat
    13261 aaaaaatcca gaatattcta acataaatgg agtattctta gaatcaaaag gagaaagaag
    13321 atggtttata cttatacgga gcaagaaaag atgaaagttt tagacacatt catgaatgat
    13381 ggaaaagtaa aacaagtacc aagttcaaca aaaaagaaat acatcctatt gaaagaagtc
    13441 ttgaaacgct ttgaccatgg agtgacttat acagaaacag aggttaattc gatactgctg
    13501 aacgtatttt ctagtggcga ttatgtcgaa caaagacgtt atctaatcac atttggcttt
    13561 ttcaaaagaa gttcagacgg tcgggcatat cagatgatgg gaattgaaaa ttagaagttt
    13621 taatttatac tcattgaaaa aagttcaagt atgcgatatt ggttctacac cgcatacttg
    13681 aacttttttt gtcatctgta tctataatac aaagccaaga acgttattca aaatatggat
    13741 agctatgata aaagctaatg agcgattcgc agttaaataa gcaatcgtca gtaaaatcgt
    13801 tccaggagcg tacatgaaaa aatcaattgg atgcgttaca tgaataaaga taattctctt
    13861 ttatttgtac gaataaatac caaaacgtac ctcctaaaat tagattttgg acagatgccc
    13921 tttaaaaaga taacaagatt tcgttcttga aacaatatat atgcttttat tccaatgagg
    13981 ttgtgaaaac agtattttgt cctgagcgtt agaaaattga aaatggggaa tagcaactcg
    14041 tactttacca ttttttaagt gaatacaaga tcagctaatt gaacaccgtt tattcgtgat
    14101 gagggacaca acactgcagt cgacacattt ctcttttatg ttcaaagata acaaatattt
    14161 aaaacaaaga aaaatagtag ataatcccgt cctaactctt ttattcttcc ctcatagctt
    14221 ccaaatttaa gcaatacttc aattaatcaa gttttatctt tttttttcaa taatcgtaaa
    14281 aaaagagtat tttttattta aaaataaaat ctttgtatta aaggacaaga ctaaaataac
    14341 cttagaataa aaagagaaaa aa
//
LOCUS       DACDYM010000067        14118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDYM010000067 DACDYM010000000
VERSION     DACDYM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.067"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..556)
                     /locus_tag="IWM59_002049"
     CDS             complement(<1..556)
                     /locus_tag="IWM59_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP9980073.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="IWM59_002050"
     CDS             complement(572..1051)
                     /locus_tag="IWM59_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9980074.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="IWM59_002051"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="IWM59_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="IWM59_002052"
     CDS             complement(2098..3204)
                     /locus_tag="IWM59_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9980075.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="IWM59_002053"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="IWM59_002053"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9980076.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="IWM59_002054"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="IWM59_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9980077.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="IWM59_002055"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="IWM59_002055"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9980078.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="IWM59_002056"
     CDS             8175..8873
                     /locus_tag="IWM59_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9980079.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="IWM59_002057"
     CDS             complement(9041..11680)
                     /locus_tag="IWM59_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9980080.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="IWM59_002058"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="IWM59_002058"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9980081.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..>14118
                     /locus_tag="IWM59_002059"
     CDS             13523..>14118
                     /locus_tag="IWM59_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9980082.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3096 c         2543 g         4520 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagc
//
LOCUS       DACDYM010000068        14026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDYM010000068 DACDYM010000000
VERSION     DACDYM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.068"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            140..460
                     /locus_tag="IWM59_002060"
     CDS             140..460
                     /locus_tag="IWM59_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980083.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IWM59_002061"
     CDS             912..1112
                     /locus_tag="IWM59_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9980084.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IWM59_002062"
     CDS             1259..4048
                     /locus_tag="IWM59_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9980085.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IWM59_002063"
     CDS             4095..4622
                     /locus_tag="IWM59_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9980086.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IWM59_002064"
     CDS             4643..5833
                     /locus_tag="IWM59_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9980087.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IWM59_002065"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IWM59_002065"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9980088.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="IWM59_002066"
     CDS             8224..8898
                     /locus_tag="IWM59_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9980089.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="IWM59_002067"
     CDS             8895..9236
                     /locus_tag="IWM59_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980090.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="IWM59_002068"
     CDS             9266..9625
                     /locus_tag="IWM59_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9980091.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9828..10007
                     /locus_tag="IWM59_002069"
                     /pseudo
     CDS             9828..10007
                     /locus_tag="IWM59_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="IWM59_002070"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="IWM59_002070"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9980092.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="IWM59_002071"
     CDS             12541..14007
                     /locus_tag="IWM59_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9980093.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4649 a         2429 c         2998 g         3950 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gttaca
//
LOCUS       DACDYM010000069        13945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDYM010000069 DACDYM010000000
VERSION     DACDYM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.069"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            950..1423
                     /locus_tag="IWM59_002072"
     CDS             950..1423
                     /locus_tag="IWM59_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9980094.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="IWM59_002073"
     CDS             1432..1659
                     /locus_tag="IWM59_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9980095.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="IWM59_002074"
     CDS             2295..2666
                     /locus_tag="IWM59_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980096.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="IWM59_002075"
     CDS             complement(2922..3164)
                     /locus_tag="IWM59_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9980097.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="IWM59_002076"
     CDS             complement(3196..4098)
                     /locus_tag="IWM59_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9980098.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="IWM59_002077"
     CDS             complement(4111..4299)
                     /locus_tag="IWM59_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9980099.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="IWM59_002078"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="IWM59_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9980100.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="IWM59_002079"
     CDS             complement(4914..5807)
                     /locus_tag="IWM59_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9980101.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6823)
                     /locus_tag="IWM59_002080"
     CDS             complement(5885..6823)
                     /locus_tag="IWM59_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9980102.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6857..7207)
                     /locus_tag="IWM59_002081"
     CDS             complement(6857..7207)
                     /locus_tag="IWM59_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9980103.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7240..8142)
                     /locus_tag="IWM59_002082"
     CDS             complement(7240..8142)
                     /locus_tag="IWM59_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9980104.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8135..8992)
                     /locus_tag="IWM59_002083"
     CDS             complement(8135..8992)
                     /locus_tag="IWM59_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9980105.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9326..10135
                     /locus_tag="IWM59_002084"
     CDS             9326..10135
                     /locus_tag="IWM59_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9980106.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10175..10672
                     /locus_tag="IWM59_002085"
     CDS             10175..10672
                     /locus_tag="IWM59_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9980107.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11057..11130)
                     /locus_tag="IWM59_002086"
     tRNA            complement(11057..11130)
                     /locus_tag="IWM59_002086"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11094..11096),aa:Ile,seq:cat)
     gene            11317..11640
                     /locus_tag="IWM59_002087"
     CDS             11317..11640
                     /locus_tag="IWM59_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980108.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11804..12058
                     /locus_tag="IWM59_002088"
     CDS             11804..12058
                     /locus_tag="IWM59_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980109.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12128..12373
                     /locus_tag="IWM59_002089"
     CDS             12128..12373
                     /locus_tag="IWM59_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9980110.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12449..12814
                     /locus_tag="IWM59_002090"
     CDS             12449..12814
                     /locus_tag="IWM59_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980111.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12875..13438)
                     /locus_tag="IWM59_002091"
     CDS             complement(12875..13438)
                     /locus_tag="IWM59_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980112.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4088 a         2577 c         2304 g         4976 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc atctttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaagatat tggcgttaat tttttttcga tacgattatc tgactgatct agattaatca
     6601 ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc cgagaaactt
     6661 cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct tcgattgctt
     6721 gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt ttttcatctg
     6781 tttctattac attcttttct tcatccacaa agtagtatct caatgtatac ctcctttttg
     6841 gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta taatcgagta
     6901 ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc atgttcaatc
     6961 gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat ggatgctcga
     7021 ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa aagctgcctt
     7081 ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc gcgagtgcga
     7141 tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta gacattgtaa
     7201 tttccattcc tactccccct cttcagttgt tgtcttctat tatttaacaa ctacatctgt
     7261 ttttggttga acttttgcat attttggata tttagggaat ttagctacac aacggaaagc
     7321 cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat ccttgctcca
     7381 cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt cttcgtattc
     7441 acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc cttgtgcatt
     7501 ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg catcttggtc
     7561 atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt gcgtaatatt
     7621 ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat aacgcattga
     7681 tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt caacagctgc
     7741 tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta aagcgatcat
     7801 gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc
     7861 aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg taaagaattc
     7921 tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca ctgagaagcg
     7981 taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc gaacaaattt
     8041 agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg gtacgaattt
     8101 tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg cctactttcg
     8161 ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga tctttcaacg
     8221 tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca tttccttctt
     8281 tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct
     8341 gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca ttcaaagcat
     8401 cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata gttttttctg
     8461 taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa ttgaagtaca
     8521 agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga tcgataatag
     8581 cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg tctagtacta
     8641 acacatctga atctaagtaa aggactttct tataatcctc tttagctaat aagtttggta
     8701 aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag tcttcgtaga
     8761 tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta tttaccgttt
     8821 cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa aattttatgc
     8881 gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag taaggagcat
     8941 aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc atcaaaatca
     9001 ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc atttggtttt
     9061 aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat cacaaaacgc
     9121 atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa
     9181 ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca taatgacaaa
     9241 gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg aaaatagata
     9301 tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag acggtacctt
     9361 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
     9421 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
     9481 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
     9541 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
     9601 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
     9661 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
     9721 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
     9781 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
     9841 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
     9901 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
     9961 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag
    10021 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
    10081 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga
    10141 ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa
    10201 tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg
    10261 tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga
    10321 tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct
    10381 tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga
    10441 aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga
    10501 agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc
    10561 ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga
    10621 atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct
    10681 atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta
    10741 cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc
    10801 acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg
    10861 caaaatatga ggaactattt ttccgtttgt taagctaata ctcaggtcaa aacgctgatt
    10921 tcacaacctc ttccttaaac ggtattcaaa agcttgcatc tgcagtaata agaacagcca
    10981 agtaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    11041 ttttcacaac ctcttatgta ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
    11101 gtctgctcta accaactgag ctaaaggtac atcataaata aaatttattt ttgttacttt
    11161 tcaactatag cacgaatgac aaactctcac aagtttcaaa atataagtaa aataatattg
    11221 ttattcttat ttcttttact tgtgtcataa ctattttgtt actttaattt ttatttgctt
    11281 attttagaaa aaatgttatt cttttattta ttctcaatgt taggagtgat tattttgcca
    11341 tttattgcga ttttattcga tttagttgcg gcagcagctt attttctcca attaaattat
    11401 caaacctcag gaattctgtt tattggaatg atttttcaag gtgtgattac ccttttgctt
    11461 cttgtcatga tgctcagcta taaaggaaaa agatatgccc gtattcaaac aaaagtattt
    11521 gttaaatatg tttccattcg ctatgggatc atcattcttt cctttttagt gaatgcgatc
    11581 gtcctctttt tatatgtatt gaattactta gatatcaatc cattgatttt ttctcgatag
    11641 ataagacaca ttcagcggaa cagttcctgc ttatcagcag aaaactgttc tgctttttca
    11701 tttttcattg atctattctt cttttatttt cctagcattt catttgtgat gtatgcaata
    11761 acatgttatc ttttattata tgaatcgttg aggagtccgt tacatgatta cgttggtgag
    11821 agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc ttatgaattg
    11881 gaacaaagaa cggttttatt tagctatttt gactcttccg gctatctact ttatgtggaa
    11941 agtttttaat tacaactact ttgaacccga tagtgttttt atagaagaat tatcaggact
    12001 ggttttgtct ttattgatcg tcattcttta tttgatacgt ttaaataaaa aacattgagt
    12061 actattgatt gcatgtgtag ctttctatag tcattataac tattctacta taatgaaagg
    12121 tgattaaatg agtatcgaag aagctatttt actggatact ttacgttgtc gtctgaattt
    12181 cttaagaact ggccgatctt tatctcaagt agctgtttct agcgcgttaa gtattccttt
    12241 attcaaatat cgtaagtacg aagaaggaga agaaattcca agcatcattg aattaatcta
    12301 tatcgctgac ttttacgata tttccgttga ttacctaatt ggtcgctcag aaacagcaag
    12361 caaactgaag tgattttttc cagccttggt gctggttttt tcatttaacg tctaaggtta
    12421 acaatctata tataaaggag acatttctat gcgattatgg catgaaaaat tgatttcaaa
    12481 gcttcctcgt caacagcttt taggacaaca tcgggaatgc tgtgcattaa gaggaaatgg
    12541 ctggggaaag aaacatgcta ctgtggatta tgtttttcac tattctcctt atagattatt
    12601 ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt ggttacagcc cagcaccaga
    12661 gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca ttttttgatt tacctactgt
    12721 agagcgcaac gtgccgattt atccggaaca cgatgaagtt tatctagcag aatgtttagc
    12781 aaatttggag caaaaaggaa tttatcttac ctaatataac tagaaaagcc ttctatctcc
    12841 tcttacttca ttaaaaagct ttaaccatct tagatcaagc taaaatgtcg taagttttag
    12901 ggttatcttc atcctcacgt gcaaaactaa aagcgtactc tagttttggt tcatcgccaa
    12961 cttccctaga cataaatgaa catctcctga cgtggtttaa aagtaccgca tcttcgacgt
    13021 ctgtatacga tgcacagcac agattgcttc ttttgatctt tcttttgatt tttctgttgc
    13081 ttcttctaca ccttcttcga ttactacatc gatgcttaca attgcttctg cctaatattc
    13141 ccatgcatcg tcgatttaag cctctgtagt cactgtgatc gtttccacat tttaactcct
    13201 ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc taagccagag tcagaacaac
    13261 ctgccattgc tagcgttaat attgatagta aaaatagttt ttttatcata aggttccccc
    13321 cctgaaaata gactaaaata ataacgcttt ctttttttgt tttcagacga aaattaataa
    13381 tttctaaata ttctttaaaa aagcttataa ctattatgcc ttttttaata tataacacag
    13441 ttatttttaa aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata
    13501 aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc
    13561 tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc
    13621 gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac
    13681 ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa
    13741 ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat
    13801 tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca acatcacttt
    13861 attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact
    13921 attcagccca ttcaacgacc aatga
//
LOCUS       DACDYM010000070        13843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDYM010000070 DACDYM010000000
VERSION     DACDYM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.070"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            57..431
                     /locus_tag="IWM59_002092"
     CDS             57..431
                     /locus_tag="IWM59_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9980113.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(545..1174)
                     /locus_tag="IWM59_002093"
     CDS             complement(545..1174)
                     /locus_tag="IWM59_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9980114.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1308..1380)
                     /locus_tag="IWM59_002094"
     tRNA            complement(1308..1380)
                     /locus_tag="IWM59_002094"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1345..1347),aa:Thr,seq:ggt)
     gene            1594..1667
                     /locus_tag="IWM59_002095"
     tRNA            1594..1667
                     /locus_tag="IWM59_002095"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1628..1630,aa:Asn,seq:gtt)
     gene            1622..1771
                     /locus_tag="IWM59_002096"
     CDS             1622..1771
                     /locus_tag="IWM59_002096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980115.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2264..3592
                     /locus_tag="IWM59_002097"
     CDS             2264..3592
                     /locus_tag="IWM59_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9980116.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3794..4627
                     /locus_tag="IWM59_002098"
     CDS             3794..4627
                     /locus_tag="IWM59_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9980117.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4643..5380
                     /locus_tag="IWM59_002099"
     CDS             4643..5380
                     /locus_tag="IWM59_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9980118.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5535..6503)
                     /locus_tag="IWM59_002100"
     CDS             complement(5535..6503)
                     /locus_tag="IWM59_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9980119.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6735..7568
                     /locus_tag="IWM59_002101"
     CDS             6735..7568
                     /locus_tag="IWM59_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9980120.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7602..8441
                     /locus_tag="IWM59_002102"
     CDS             7602..8441
                     /locus_tag="IWM59_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9980121.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8467..9027
                     /locus_tag="IWM59_002103"
     CDS             8467..9027
                     /locus_tag="IWM59_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9980122.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9226..10947
                     /locus_tag="IWM59_002104"
     CDS             9226..10947
                     /locus_tag="IWM59_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9980123.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11112..12254
                     /locus_tag="IWM59_002105"
     CDS             11112..12254
                     /locus_tag="IWM59_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9980124.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12270..13481
                     /gene="thiI"
                     /locus_tag="IWM59_002106"
     CDS             12270..13481
                     /gene="thiI"
                     /locus_tag="IWM59_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9980125.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4727 a         2288 c         2822 g         4006 t
ORIGIN      
        1 tttattaata tatgctagca tgcttttgta agaaaaagaa agagggcgta ttagttatgt
       61 atcttaaaat tacggaacaa gcacaagaaa gattacaaaa atatattgat gaaggcgcaa
      121 cagttatatt ggatttagat gatggcgttg gggaatattc taaaatgggt gtttgctctt
      181 tagatactag ttttcgttta cttcttcttg ataaagaaca gagaaaaaat gattataaac
      241 tccaattaga tagtgatatt ggggatgttt atataaaaga ttattcaaaa atgtacatgg
      301 atgaacatat gaccctttct ttagatccac gtctaggtgt atttactctt gaaagcccaa
      361 gtggttcttt agacagtcat gttcaattag tagatctacg accagaaaaa ttctctggaa
      421 taaaacaata actttctctc tattgtccat aaaactagtc tcaatcaagc tgtattgaaa
      481 aaacaagaca acattcttac catggtaaat aaaaagacga cacattttgt cgtctttttt
      541 taaattaact attttcgata aaagatcgca tagcttgtgc cattatttct ggcttttctc
      601 tattcaattc atgttttccg tttttaataa agagtagccg gccattcgtt gaaagtttat
      661 ataaacgttt tgctgctggt ttattcgcat gatctttggt accgcataaa atcaatttag
      721 gaagtcgtga tgtacgaagc tgttttttgc agtctaatgt ttcaagtgaa cgaagtaggg
      781 taagggtttg ttttttagat agacctaatt tttcaaatat cttagaaggt acgcagtaaa
      841 aataaatatt ctggaaagta agcaataagc gattaggaga ctcaaattgt ggagcgacta
      901 aaacaacctt ttttaaatat ggagaaggag ttgcaagata gtttaaagca ataacagcgc
      961 ctaaagataa tccacataaa ataaaaggtt cctttatctg attcaattgt acttcgatgt
     1021 atttggttaa gtcgtctaaa gtagcagtgt ctgtgctgtt taaatgattg aacaatgaaa
     1081 gacttttgca agaataacta ttgggtagat aagagattac atctttccat gattgttcgt
     1141 cttggcctag accatgcaaa aaaacaattt tcatattgct ctccttttta tagttatcat
     1201 atagactttc tttgtactat taaaatataa acattcattt cttacgtcat aaaataaaaa
     1261 aacagcgcag acacattgtt aaaccaacat gttgcactgt ttaaggaagc cacttgccgg
     1321 gcttgaaccg gcgacctctt ccttaccatg gaagcgctct accagctgag ctaaagcggc
     1381 attatcatta atacaaataa taggataacg aatattaatg tatttgtcaa cagaattttt
     1441 tcgaaaaaat gtgtttctct aataggaaaa cagattttag aaaaaataaa aataacaaaa
     1501 aatgcgaacg gtttctttga taatataata aaaaaattca aaaaaagtat tgcgttattt
     1561 agaagaaatt gatattatat tcatgtactt gattccggca tagctcagtt ggtagtagcg
     1621 catgactgtt aatcatgatg tcgtaggttc gagtcctact gccggagtaa ataaagctta
     1681 tgcaaagaaa aagaaatcca atcacttggg tttctttttc ttttattatt atgatgaatc
     1741 aagctttctg tttttgtcac gtatgttgta atctttacga aagagtagag ttggctactc
     1801 ataatcaacc atacttccca tcatcgctaa atacgaaata aatcagcatt cctacaccaa
     1861 tcaaagcaca agctgcacca agaaaaggga tgacattccc aaagttatct gtattcataa
     1921 gtatgccacc acccacaaac aaaaagccgc cagcaatcag gtttttcatt ttattcatct
     1981 cctttcttac gttatagtat ttccttcttt caatgagtat tttgaaagct atcttattgt
     2041 atgatgttct tctaggcctc ttctttatta tacaggccat tttcgttatc tcatagaaat
     2101 attccttttt atctgaaaac tattgtatac cgtatgacat ttttcttcat gaagttgtaa
     2161 gtatattctc attcaaatga aaatttcatc atagaagaga ttgtatttct aaattcttaa
     2221 gagtaaaata cgaagtataa cttggatttg gaaggatgat tggatgaaag ctattacgcc
     2281 tgatatgaca acaaaattta acaaacagtt tcaagcaaat atcaaacagc aggcattgca
     2341 gcgggcagtt gtgaagaacg gaattactgc ttctgctgaa aatgtttcag cacatgtgga
     2401 gaatacaccg gtattttcta tagatttggc tacggggaaa gtggctaatc aaatgcaatc
     2461 ggggcgttgc tggatgtttg ctgcactgaa cacattccgt cataagatgc tgaatcaatt
     2521 ccatttaaaa gagtttgaat tatcacaaaa ttacactttt ttctgggata aatatgaaaa
     2581 ggcaaattat ttctatgaaa acatcattgc aacaggaaaa gaggcaatcg atagccggaa
     2641 agtagctttc ttactcgcta ctcctcaaca agatggcggc caatgggata tgatcgtttc
     2701 attattccaa aaatatggcg ttgtgcctaa atcggtgatg ccggaaagtg cgaatagttc
     2761 aaattctcga gatttgaata actacttgaa caaattactg cgtaaagatg cagtagtgtt
     2821 acggaaaatg gttgctcaag gagaaacatt agacgctatt gaagaaaaga aagaagaaat
     2881 gctagaaagc atttataact tcttagctat ctcattagga acacctccaa aagaattcga
     2941 ttttgagtac cgcgatgaag aaaagaacta tcatcttgac cgaggactga caccacaaat
     3001 tttttatgat aaatacatcg gtgtaaaatt agatgattat gttagcgtga tcaatgcacc
     3061 aacaaaagac aaaccattca atcgttctta tacagtagag atgctaggaa atgtcgttgg
     3121 cgggaaagaa gtcaaatatc tgaatgttga tatggaaact ttcaaaaaat tagcgattgc
     3181 tcagcttgaa gaaggagaat ctgtttggtt tggttgtgat gtaggtcaat cttctactag
     3241 aactagtggc atcatggcct tagatgctta ttcaatggat gatctatttg atacggattt
     3301 tacaatgact aaagctgaac gtttagattt tggagaaagt ctgatgaccc atgcgatggt
     3361 attgaccggt gttgacttag tagacgggca atcaacaaaa tggaaagtcg aaaatagctg
     3421 gggcgaaaaa gttggaaaga atggcttctt tgttatgagt gatgcctgga tggacgaata
     3481 tacgtaccaa atcgttgtaa gaaaagattt attgacgaaa gagcaattaa atgcgttcaa
     3541 tgaagaacca attgttctat ctccgtggga tccaatgggt gctttagcat agacgagtat
     3601 ttcaatgaag agtatgtagt aaagataaaa agagctggct taagccagct ctttttatct
     3661 ttaaagtcca ttccctgttt tttatggtcc ctgtgctata attctaaata aaggataagg
     3721 attggaaata atccttttat cattaaaaat atgatgacga agtagaataa aattgggagt
     3781 aggggggaaa gtcatggata tccaaactgt tttatctgat ctgaaattaa atggtaaagt
     3841 gattccagtt gttggtggcg atgttaatca gacctatcgt ataaaaaccg aacatcgtgc
     3901 ttatttttta aaaatccatc cgaatgtaaa aaaaggattt tttgaagcag aagtagacgg
     3961 attaaaagaa ctgtcagcat tcgtacgtgt accggatacg tatatgctag gtgaaacaag
     4021 tgaaggcgct tatttgttaa tggaatggat cgagccggga aaaggggatc aacgagatct
     4081 tgctgcagct ttagctaatc tccatcagca aacagcgcca caatttggtt ttcgtaaaga
     4141 taactatttg ggtacacttg ttcagaaaaa ttcttttgaa gaagattggt ggactttttt
     4201 ctttaaagat cggcttgaat cccagatttc tttggcagaa gaaacgaatc gatggaatgt
     4261 acagcgtcaa gaaaagtatc tgcgatttaa agaacgtgta ttgaaatcgg ttgaaccaaa
     4321 aaagatcact ccacgcctct tgcacggaga cctgtggagt ggtaatgtgt tctttgacca
     4381 gcaagggcat ccagtttttg tcgacccagc tgtttcttat gggaatcggg aacaagatat
     4441 tgcaatgagc cagttattcg gtggtttccg accggaattt ttagatgctt atcaaactat
     4501 atttccatta gaaaagggat ggaaagaccg actgccgatt taccagttat attatctttt
     4561 ggcacatttg aatatgtttg gagaaagtta cggttcccaa gtagatcaat tactagaaaa
     4621 tttttaagag gagggcgtgt ttttggcaaa aaaatatacc aaaaagcatg aagtttcata
     4681 ttatgagtgt gacatcaacc aaacgatgac atttccttcg atgttaggag tagttatcaa
     4741 aacgtctgaa gatcaaagcg accagttggg aagaggcagt gaattcgtca atagctttgg
     4801 acttacttgg gtcatcacga attattccat ggagattaat cgtctcccta gagtcggtga
     4861 agtaatctca gtaacgactc aagccatgga gtacaataag tttttctgtt atcggaactt
     4921 ctggatccat gatgaagatg ggaaagaatt agtcaaaata gaatctgttt tcgtattgat
     4981 ggattttgtg aatcgaaaaa tgagcagtgt taatgaagag atcattgctc cttttgagag
     5041 tgagaagatc aagaaaatca agcgtcagga aaaaattgaa aagattgatt cgggcttcat
     5101 gttgccatat cgtgttcgtt tttatgatat cgatagtaat cagcatgtta ataatgcgat
     5161 gtattttaat tggatcattg acgtattagg atatgacttt ttgactacgc atgttccaga
     5221 aaaagtaatt attcgatttg ataaagaagt agagtacggg aatgagatcg aaagccattt
     5281 tgaacaaatc cctatcgaaa atggtgtcaa aacaaaacat gaaatccgaa tgcaagaaca
     5341 gctgtattgt gaagcaaata ttatatggaa aaataaataa aaaagaggtt gtgacagaag
     5401 ggggcaactt caagaaataa gaagaaattt acgaaaattg tttctcaaat ttttgtaaat
     5461 tttcgactta gttttcgaag aagctatttt tgtcacagcc tctcttttta agtttagcta
     5521 ttagataatg atgtttagtt ttggataaac gaaatcaatt cttcacccga caaaccatag
     5581 tttacagcaa tcaatagttt taaacgtgct ttttgactat taatactgtt acagaaaata
     5641 atccccattt tttctagttc ttttcccccg ccttcataat cataaacagg ctctgcgatt
     5701 ccattgaaac aacgagaaac aagtactaca gggatttttt ggacaagaag cttttccaaa
     5761 gcagctaagg cttgtggagg gagattcccc gcacctaaag cttcgattac caaaccgtct
     5821 actttcgtat gggcgatggc ttccaataag tccccttgca tcccagcata tgctttaacg
     5881 atcgggatag tcccatctac agcggaaatg tccagtcgct ttgtttctaa aagttcctgc
     5941 aagaataaaa ttcgattctt agttactaag ccgatgggac ccaatgtagg tgttcggaaa
     6001 gtagcaacat ttgtggtatg ggtctttgtc acgtagcgtg cagaatggat ctcatcgttc
     6061 atgaccacca aaaccccctt atctagcgct tcttcacaac ttgcgacacg aatcgcactt
     6121 tcaaaattat aaaggccgtc acttccaagt tcattacttg aacgcattgc cccagtcaaa
     6181 acaattggta atttgttgcc aatcgttgta tcaagaaaaa aagcagtttc ttctaatgta
     6241 tctgtaccgt gtgtgatcac cactccggat atacctgatg cataagcgga caagatacgt
     6301 tcttttaata ccaacatgtg agaaggcatg atgtgtggtg agggaagatt gaagatatct
     6361 tcaactaata attctacatg cttggggatt gtgatttctg catctaataa tgggtttgcc
     6421 acatcaggac taacaccgcc cgtgagcatg ttttctttca tggcgatcgt tccgccggtg
     6481 tgtagtacaa gtattttttt cattttacag acttccttac gaataatttt gcatagccgt
     6541 taaatcttat tgtatactat ataaaagagt tttgctatca gtgaagaata acgaatagcc
     6601 taacttatca taaaaaagct gaccttatta aaatggaaaa gactcaacct tgacctccta
     6661 atacaatgga gtatctggtt gagtttcttt tacaagaaag cagccaagga aatcaatcac
     6721 gaaggtggcc ggaaatgatc agaatcggat taacatcgtt taaagaacat gacaaattaa
     6781 ctggtaaaaa gaataacact ctttatgaat atgcagggtt tctaccctta gtggaattag
     6841 atactgccta ttatggaatt ccccgaaagt cctcagttga gaattggata agtgaagtac
     6901 ctgaatcatt tcgctttgta gtcaaagtct atcaaggaat cagcggtcaa ggagactgga
     6961 agccttatta tcagaccgaa gaagagatga ttgcgcattt tttagatgtt atgtcgccaa
     7021 tggtagatgc tggaaagatg ttttgctttt tagtccaatt cccctcatca ttcaaatgta
     7081 cgaaagaaaa tgtccagtac ttgcgcaaac ttagaaagtg gtttgagggt tatcctgttt
     7141 ctatagaatt gcgggattat tcttggtaca gcgatgaatt tcttgaacgt acaagaaact
     7201 ttatgtctag agccgctttt tctttagcca tcatcgatga gccacagttg ctgaatacaa
     7261 cgattccttt tgatccattt gtcaccaatc cgaactttgc tctttttcga ttccatggta
     7321 ggaatgcgca aggatggcaa gcaaatgata aagagtggcg taaaaaaaga acgttatatc
     7381 gttattcaga aaaagaactg caagaactag cacatgaagt caaagtagtt gattctaaga
     7441 caaaagaggt aggtgtgatt tttaataata attctggtgg tgatgcggct gaaaatgcat
     7501 tagcactgca aaaaatatta aatatcaagc cagatggact caatccaacc caaatggatc
     7561 tgttttaagg gcttatttta ttcaagaaag gggcgaagtc tatgatcaaa gctatttttt
     7621 ttgatattga cggaacattg gtcaccaatc gttcaaaagc gttggaatca acaaaacaag
     7681 caattgaata tgctcggaaa aacggcattt tatgcggtgt agcaacgggg cgcagcccag
     7741 taaagatcaa agaaatcatt gatgaattgg aattagatat gtatgtcgtg tataatggtc
     7801 aactggtttt tactgcagat cgcacgatta tcgaccaccc ttttgaacaa aaagtattgg
     7861 aacatattgt tgaatttgca gatgagaacc atcgacaaat cgtttttggt gcgagaaatc
     7921 ggttagatgg atcaacgacg atgctattag gacaatcgat ttttatcaag cgactagtca
     7981 gctttttacc gcgtaaattc ccggtacgtc tgatgaaaaa aatcctccag gtttttagcc
     8041 ctcaccgaca aaaggatcgt tatgaaacgt tagagatatt aaaagaacca atttatcaat
     8101 gtatattgct cagtccggag tcagaacaga aaaagttaga acatcgcttt cccgagtgta
     8161 cgtttcagcg ttcaaatacg tatactgtag atatcatccc aaaaggtggt tcaaaattac
     8221 taggcatcca agctttcgca aatgctgtcg aaattgaaat ggaagaaatt atggcattcg
     8281 gcgatcatta caatgatatc gagatgctga aaggtgtagg aattggagtg gccatgggga
     8341 atgctcagat cgaagtaaaa catgctgcag actttgtgac tcaatcaaat gaagaagatg
     8401 ggatatacga aggactgaaa cacttcggtc tgatttatta gtcaatgaaa gaaggaaaaa
     8461 atcagtatgg aagcagagac accttttcaa aaagtagaaa cattccacaa aacttttgat
     8521 ccaagaagac cagagatacc cacggctttt acagaaaatg aagctcatac acgtgctgcg
     8581 ttcaaaatag aagaacttgt tgaatttgtt gcagctactt cacctaataa agaacaactg
     8641 catgaagctg ttatgctgct gcacgaagca ttagataaag cagaagaaaa aatacttggt
     8701 aaaacacagt ggggaagtga tccgctggtt gaacaagtag atgcactgac agatatcctt
     8761 tactttactt atggttcttt ttcattacta ggaatagtgc ctgatcgaat attcgaaatc
     8821 gtccatcagg caaatatggg gaaattattt ccagatggaa aacctcatta tgaccctgta
     8881 acaaataaag tgttgaaacc tgaaaactgg cagatggaat atgctccaga acctaaaatc
     8941 gcggcagaaa tccaacgtca gattcaagaa gcgattgcta agaagaaatc cgctgatata
     9001 ggcatgtgca caaatgaaaa agagtgatca gagtttcact atttttgtta tctttatacg
     9061 atggaaaaac aaaagatttt cgtttaagtt agtgaatgca tgaaaatagt atgctataat
     9121 ctagaataga tgtcttttgc acgagagacg tattatttgg atttatgcca agcagaacgc
     9181 catctgttgc ataaaagatg catactgaaa acggaggaac cataaatggg aaataatgtt
     9241 atcatcggaa tcgtcattgc aatcattata gtcgccattg tcgtcttttt ggttagttac
     9301 ttgatgaaga aaaaaaacca agatcgattg gatgccttag aaaaacgcaa ggaagaatta
     9361 tttgacttgc cagtgattga agaagtcgat gatgttaaaa aaatgcatct ggttgggcaa
     9421 agccaaaata cctttcgtga atggagtcaa aaatggaatg acatatctac ttcctcgttc
     9481 gctgaactag aaagtcaaat tttcgaagtg gaaaacctca atgaaagttt tcggttctta
     9541 aaaggaaaaa aagcagttgt tgaagcagaa gctacaatgg accaaatgga gaaacaagtg
     9601 acggaaatcc gtgaaggtct aaaggaattg cgggaaacgg aagaacgcaa ctcactagct
     9661 gttcagcagg cacttgacgt ctatgaagaa ctgaaagaaa aagtcagaga agacgagcat
     9721 gaatacggac cagctctttt tgaattgcaa aaacagatca aagtgatcga aaaagaattt
     9781 acgcagtttg tgactttaaa cacttctgga gaccctgttg aagcccgcga ggttttggat
     9841 aaagcagaaa aacatacgta tgaagtggaa gctctgatga aaaaaattcc accgctgtat
     9901 gaggagctcc ataccacttt ccctgaacaa cttgaagaaa tcagtgatac ttacgagaaa
     9961 ttgatggaag aacaatatgt attcccagaa gaagatcttg ctgaagatat cgcaaaagta
    10021 agtcgaagaa ttgaaaattc attagccaat cttgaaaaaa cagaagttga aacggtcgaa
    10081 tttgaaaacc gtgaaacagc tgatttgatt gattcgttat atgatatctt agaacgggaa
    10141 atggaagctc agagatatgt aaaaactaac caatcaacga ttgcagagta tatcaaacat
    10201 acaacaaaga ataaccgtca attattgatt gagctggatc acacatctca aagctataca
    10261 ttgaaccaca atgaaattgg ccgtgtacga ggtttccaaa ccgaagtgga agaaatggaa
    10321 cgtcaaaatg aacagatgat tccacagatc cgtcaacacg aaattcctta ttcagaagtg
    10381 cgcacgttct ataaaacagt cttcaaagtc ttagaagata tcgaaaagca acaagtagag
    10441 atcgacgact cattacatga attgagaaaa ggcgaaaaag aagcgcaaga aaagatcgat
    10501 acattcgaat tcaaattgcg cagtttgaaa cgttttgtag aaaaacaacg tttgcctggc
    10561 ttgccgaatg attacctaga attctttttt gttgctactg accgtattga agaactaagt
    10621 gttgtcttga ataaaattcg agtaaacatg gaagaggtca atcgtttagt tgctctatgt
    10681 gaagaagatc tagaattatt agataagaag acacatgatt tagtagatgc agcagcatta
    10741 acggagcaaa tgctccaata tgcaaatcgt tatcgtcata cacacgaaga agtgagagaa
    10801 gcaattgata aaagctacta tttattcaat aaagaatatc actatcaaga agcattagac
    10861 gaaatcggta cagcattaga gcgagtagaa cctggtgctt tcaaacgcat cgaagatttt
    10921 tattttaatc atccagactt agtataaagc aaaaaagtta gtaaaccagc aagagcaaga
    10981 cggattattg tcttgttctt gtcttttttt gtatatttga aaattgaaac aagtgaaaat
    11041 ttcacctgct cttgaaagca ccttggacaa ccgttataat agaaaagaat ttcaaaaaag
    11101 aggggtttct tatgatatat tttgataaca gtgcaacaac cccaatcttt ccgcaaagct
    11161 tagatgcata tgtaaaaacc agtcagcgga ttattgggaa tccatcaagc ttgcatgatt
    11221 taggcaatca agctaaccgg ttgttgcagc aagcaagaaa gcagatcgct gatttgatcc
    11281 atgtagaacc agaagaaatc tattttactt caggcggaac agaaggagat aactgggtat
    11341 taaaaggaac gatgatcgaa aaaagagaat atgggaatca tatgatcatt tcaggagtcg
    11401 aacatccggc cgtcagcgag acagcagaac aattaaaaga attaggaatc gaagtaagta
    11461 tcgccccagt ggataaacgc ggatttgtac gtgtcgacga attgaaagaa ttaataagaa
    11521 aagagacagt attagtctct gttatggctg tgaataatga agtaggatcg attcagccca
    11581 tccaggaaat cagtgatctt ttggatgatt ttcctaaaat tcatttccat gtagatgctg
    11641 ttcaggcgat tggaaaagta ccattctcgc aatggttgac agaaagagtt gattttgcaa
    11701 cattttcagc tcataaattc catggcccac ggggaactgg tttcatttat tggaaaaagg
    11761 gtcgtcgttt agcaccatta ctgacaggtg gagggcaaga aagaaatcag cgaagtggga
    11821 cagaaaatgt tccaggtatt gtggctatgg ttaaagcttt acgtttatca ttagagataa
    11881 aggaacaaaa acctgagcag acacaacgct taaaagtagc tctaatcaat gcgttgaaga
    11941 cgtatgagaa agtgacgatt ttttcagaag gaactgacta tgcaccgcat attttatgtt
    12001 ttgctctaaa aggaattcgc ggcgaagttc tcgtccatgc cttagaagaa aaacaaatat
    12061 ttgtttctac tactagtgcc tgctcctcac gtaaacatat ggcaagcagt acattgcacg
    12121 caatgcatgt accaaatgat ctggctacat ctgctgttcg aatcagctta gatgaaaaca
    12181 attcgcttgc agaagtcgag caatttatga ttattttcaa gcagctttat aagaaatttt
    12241 ctgcaatcaa ttaaaagagg agtgttcttg tgaaatacag tgaaattatg gtccgttatg
    12301 gggaactttc gacaaaagga aagaatcgaa aaagctttat catgcaacta gcacaaaatg
    12361 tcagacaagc tttgaaagac tttccagaaa tcaaaatcca tgctgatcgg gaccggatgc
    12421 atcttttatt gaatggcgca gacgggcagc tagttattcc aaaactagca aaaatatttg
    12481 ggatccaaaa tttctctcca agtattcgtg tagaaaaaga tgtcgaagtt ttgaaacaag
    12541 ctgttcaaga catcatgaaa gaaatttata ctgggaaaga aacatttaaa attatcgcta
    12601 aaagaagcga tcaccaattt gaattgacaa gtaatgaatt gaatcaaaca ttaggtaatg
    12661 ctgtttttga tattttcccg catatccaag ttcaaatgcg tcaaccagat attccattac
    12721 gtgtagagat tcggcgtgat ggcgcttatc tatcgtatga aacgatcaag ggagctggag
    12781 gattgcccgt aggaacaagc ggaaaaggaa tgcttatgct ttctgggggg attgattctc
    12841 ctgtagcagg gtactttgcc atgaagcggg gagtggaaat tgaagctgtt cattttgcta
    12901 gtccgccgta tacaagtgaa caagcactgc aaaaagcaaa agatctaaca gccaaactag
    12961 caccttatgt gggaacgatc cattttatcg aagtgccatt taccgagatt caagaagaaa
    13021 tcaaaaagtc agtgccacaa ggatattgga tgacaattac tcgtcgaatg atgttgcgtt
    13081 tgaccgatga aattcgccgt attcgtcatg gtttagtcat tttgaatggt gaatcattag
    13141 gtcaagtggc ttcacaaact ttgcaaagta tggtggctat caatgaagta acgaatacgc
    13201 caatcattcg gcctgtagcg acaatggata aacttgaaat cattgaagta gcagaacaga
    13261 tcgatacatt taatctttct atccagcctt ttgaagattg ctgtacgatt tttgctcctc
    13321 cacagccaaa aacacgccct caactggaaa aggtccatca atatgaagag cgtttagcaa
    13381 tagatgaaat gatccaacga gcactagctg gcttgaagat ggaagagata caagcagaat
    13441 cgtctaatga aaaaactgaa gaattttcag aattgctata agaggttatg aaaatagtgt
    13501 tttgaccaga gtattagaga aacaaaacga aaaaatagct ccatgtattt taccgttttt
    13561 gaactaatgt cgaagattag ctgattgaat accatttatc aagaagttat ggaaaagccg
    13621 gttctttttt actaactgaa aaataaaatt ttatttagag atcaagctac agttgataaa
    13681 tgatataaaa aagagaattt cccatgaaat agcgaggttg tgacagaagt gggcagcttc
    13741 aagaaataag aaggaattta cgaaaattgc ttttcaaatt tttgtgaaat ttcggcttat
    13801 ttttcgaaga agttacttct ggaacaccgt gtatcagagg ttg
//
LOCUS       DACDYM010000071        13730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDYM010000071 DACDYM010000000
VERSION     DACDYM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.071"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(75..2072)
                     /gene="tkt"
                     /locus_tag="IWM59_002107"
     CDS             complement(75..2072)
                     /gene="tkt"
                     /locus_tag="IWM59_002107"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9980126.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2174..2425)
                     /locus_tag="IWM59_002108"
     CDS             complement(2174..2425)
                     /locus_tag="IWM59_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9980127.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2572..3195
                     /gene="lexA"
                     /locus_tag="IWM59_002109"
     CDS             2572..3195
                     /gene="lexA"
                     /locus_tag="IWM59_002109"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9980128.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3375..3593
                     /locus_tag="IWM59_002110"
     CDS             3375..3593
                     /locus_tag="IWM59_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980129.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3882..4394)
                     /locus_tag="IWM59_002111"
     CDS             complement(3882..4394)
                     /locus_tag="IWM59_002111"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9980130.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4408..6705)
                     /gene="recJ"
                     /locus_tag="IWM59_002112"
     CDS             complement(4408..6705)
                     /gene="recJ"
                     /locus_tag="IWM59_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9980131.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6847..7281)
                     /locus_tag="IWM59_002113"
     CDS             complement(6847..7281)
                     /locus_tag="IWM59_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9980132.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7291..8079)
                     /locus_tag="IWM59_002114"
     CDS             complement(7291..8079)
                     /locus_tag="IWM59_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9980133.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8076..9017)
                     /locus_tag="IWM59_002115"
     CDS             complement(8076..9017)
                     /locus_tag="IWM59_002115"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9980134.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9194..9781)
                     /locus_tag="IWM59_002116"
     CDS             complement(9194..9781)
                     /locus_tag="IWM59_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9980135.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10002..11315)
                     /gene="obgE"
                     /locus_tag="IWM59_002117"
     CDS             complement(10002..11315)
                     /gene="obgE"
                     /locus_tag="IWM59_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9980136.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11612..12622
                     /gene="gap"
                     /locus_tag="IWM59_002118"
     CDS             11612..12622
                     /gene="gap"
                     /locus_tag="IWM59_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9980137.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12707..13642)
                     /locus_tag="IWM59_002119"
     CDS             complement(12707..13642)
                     /locus_tag="IWM59_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9980138.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3775 a         3082 c         2425 g         4448 t
ORIGIN      
        1 tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct tatcagtcaa
       61 acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc tactgtaaat
      121 ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc gattgtaatc
      181 gttgcgccct ctgttcctac gtagcgttcc catccaaatg atgcagcagc ttcgatagcc
      241 acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga ttgtttttca
      301 aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc agccaattct
      361 ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat accttctggt
      421 gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc atctgctact
      481 tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat agtaggcgca
      541 tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc tgccggacga
      601 atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg ttcgtgcgtt
      661 ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac aggagtatgt
      721 tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa gaatgttcca
      781 ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc cattgcaaat
      841 tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa atctttatct
      901 gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca gaagcttggt
      961 atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc ttgactgctt
     1021 ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc atcaaaagca
     1081 tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa catttcattc
     1141 catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg ggcagctact
     1201 tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc catcttgatg
     1261 ccatcttcac caagaggagc accatgaaca gcagatgttc cttctttagg agcaccatag
     1321 ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt tgcttcttca
     1381 atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg ttgccagcca
     1441 tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg accgtctagt
     1501 gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg acctgccatc
     1561 gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat tgcatacgta
     1621 tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg cgcttctgcc
     1681 attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt ggcttctaca
     1741 ccatctgtat gatgtacttc aggatgtcct ggtgttttag aatcccattg acggaattgt
     1801 ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata caacattgct
     1861 gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt tgatgtcgtt
     1921 ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc gcccatcggc
     1981 aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt acgaattgtg
     2041 ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa tttaggtttt
     2101 ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa gcaatctttt
     2161 tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc ttttggattt
     2221 cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc attccttcga
     2281 tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgcaag ttgatttgtt
     2341 cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt tcattgatcc
     2401 gctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt catattatat
     2461 aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc gtttgattta
     2521 gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct tgtggcccga
     2581 caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt aacccaaaaa
     2641 ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc tacttctact
     2701 gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga tccgacgaaa
     2761 cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg tccaacaact
     2821 attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga ggatgcttca
     2881 gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt tatgctgaca
     2941 attcgtggag atagtatgat caatgctggg atattggatg gtgacaacgt gatcgttcgg
     3001 aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga ggacgaagcg
     3061 acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga aaacgacttg
     3121 ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg actttatcgt
     3181 agtcatatct attaattgct actaattaaa agaaaacttt ttaaaaagag tttgagacgt
     3241 tggtctcaaa ctcttttttc gcccgggttc ttcttctttc aaatttttat cacttccctc
     3301 tttacaaacc gaacaaatat tcgtatacta aatatacgaa caattgttcg cataaattaa
     3361 agaggtgaga attcatgaaa tcaatgagac gtcttggctt agtattaact gttttgtttt
     3421 taggtattat tattggtaat ttcggcatac gtccagcttt acttgtgttt gttccgttat
     3481 ttactttgtg gttcatgttg tgggatgaaa agaagtaccg tcaatctgag cggcgcagaa
     3541 ttcatgaaga tcatttctat catgagccct actacagatc caggcaatca taataaggat
     3601 tgactgctaa ttattcaaaa gacaagaagt taaactttta cgggggtgtg acaaaaatcg
     3661 gtcacatcct ttttttcgaa taaacggtgt tctaaagcca gtttttcggt attaagtcaa
     3721 attgcggaaa aagtgatttt ttaaacttct actggtcttt gcttgtttcc atttataaaa
     3781 aaatagcgac cctcaaaagt cagatagaga atctgacttt tgagggtcgc tttaaaatcg
     3841 cttctttaag aataacatca agaatagctt cttttttctg cttaatattc cattaatgta
     3901 atgatgtcat atcctttgat tttgtcgcgt ccatggagat ccatcaattc gatcaaaaat
     3961 gcgcagccta cgactacacc gcctaattgt tcgatcagtt cgatcgtcgc tttgattgtt
     4021 cctccagtag ctagcaaatc gtcacaaatc agcacacgtt gacctggttt aatggcatcc
     4081 ttatgcaatg tcaatgtatc tgttccatat tctaatccat aagtcacttc gatggtttct
     4141 cgaggtaact tccctttttt gcggactggg gcaaaaccga ctcctaactc ataagcaaca
     4201 ggacatccta cgatgaatcc acgggcttct ggcccaacta ccatatcaat tcgtttttct
     4261 tttgcataat ttacgatttg tttagtagct tcccgataag cgtcaccatc tgccatcagt
     4321 ggggaaatat ctcgaaacac aattcctttt gacggatagt cgggaatact ggcaatataa
     4381 tcttttaaat ccactctttg tttcctccta ggttgttaac catttcttta tggtaggaat
     4441 atcgctcaaa agcaaaaatt cctcaacttt gattttattg agccgtcgct ggtataatct
     4501 gctctcagtc aaaggatgac tttctgggct agcgactttt tgtaaaacac cgttctctat
     4561 tgtaacaaat ttcaattcaa aaaacacctg tatcataaaa attaataatt tttcagggat
     4621 acgcaaatat tgagctattg cttttatttt atgacgtata tcaattcgtt cttgagaatg
     4681 gatcaattta aacaattttg cgtactgttc tcttgtgcct accccattca aataggcctc
     4741 atcaggtgaa atccccatca gataaacacg attgaatgaa taagaccgaa aaacttcttt
     4801 cagatccgct aaatcatctg gacaatccat aacaactaga gagtttggtt ttcttttttc
     4861 aattattgcc tttacagaat ctagtccttc gaaaacaatc gcagcttcct ggataatctc
     4921 aggcacgaat ttcaacgtcg aagaatcaaa tgctaaataa aggctacctt ccgacgttcg
     4981 gctctgttca cggaaacgtt ttgcccgcca atcaaatact tgcagaccac ccacagaaaa
     5041 atcagatacc atcagctgcg gtttcttacg cccgttccat tcattgatcg aaagctttcc
     5101 taccacatct acctgattat tcagcagttc ttcttcttga gtaccaaaac caaaagcgac
     5161 cgcatcaagc tgactcgatg catcggaaag gacaaatttc agatgctgtt ggtttgctcc
     5221 gattcttttg atattttctg cttggacttg tcgaaataaa aactgaggca aaggattatc
     5281 cgtaccaaat ggcgccaata gtttcagctc atcaattcgc tctactgtta cttcatttgg
     5341 cagcaatact tcatcaattc tcaactcagg ccctctcatc aagtcaattt gatgatcgac
     5401 tatatattgg ttcatcttct cctgtagtat agtgacattt tcacttggca tcgttaaacc
     5461 gactgctgca tgatgtcctc cgaagtaagt aaataaatct cgcatctggt caagcatttc
     5521 aaaaagattc aaggcttcaa tactacgtcc agatccttta gctgatccat cttcttttaa
     5581 agtaaggaca agagttggtt tacccgtttc attcattatt ttcccagcaa caatgcccaa
     5641 aacgccttca tgccatcctc ggtttaccaa tagatggata tgattctcct tattgaccat
     5701 cgctaatgct tcttgtgtta tctgctctac gattgctttt ctttcttcat tgatctcatt
     5761 caattttttt gcttgaacgg atgcttcctc ttcatcaaac gtcgcaagta acgagacggc
     5821 aggacgtgga tcgcccatcc gaccgattgc atttagtctg ggagcaatcg aaaaaccgat
     5881 cgttgtttcg tctgcgtccc tcatttttac gccgctttct tcaaatagcg cctgaagtcc
     5941 tatccgttct gaatgatgaa tggcatcgat tcctaatgaa acaaggattc gattctcatc
     6001 agtcaatgaa actaaatcgg cgatcgttcc tatcgctact aaatccaaaa attctgcagg
     6061 cggttcttcc aataatgcag aagctacttt gaatgctact ccgactcctg ccaaatctgg
     6121 aaatggatag tgccccgctg gatgacgagg gtgaacaatc gcataagcgt ctggtaaatt
     6181 ctcaggtagt tcgtgatgat ctgtaatgat cacgtctaca ccagcttgct gagcgtatgc
     6241 taccgcatca tttcctgcta ccccgttatc aacagtgatg atcaactgga ttccttcttc
     6301 gatcttttct tggtataccg attggtttgg tccataccca tgctcaaatc gatttggcag
     6361 gaatacttct acttctgcac caagtaattc cagtgtttct ttcattaccg ttgcactggt
     6421 gatcccatct gcgtcataat caccgtaaac gaggattttt tctccactga ttacagcttc
     6481 ttggattcgg ctgattgctt tatccatatc gtgtatcaga tacggatcat gtaattcttg
     6541 ttcctttgga tgtaaaaatc ggttgatatc cttttcctca tggtaacctc tttgccacag
     6601 taacttaccg acaaatggag ataatttcag ttcacggatc atttggagaa aggattcaga
     6661 aggctctgtc tcgcttaact tccaatcaaa atttgcttgt ctcactttct cactcctaac
     6721 ttagttatta tagcaaagga aatcagcaaa aaaaagcaga aagaaagaat aaaaacagat
     6781 cagatccgct agacagatgt tcagcaggac ctgatctttt gtacgaccat ttatgaaata
     6841 tccctattag tcaaaataat tgaagctttg tttgggcgat tgagtttttt gttcttccgg
     6901 cgctttcaat tgactgattt gtgcggtttt cgcttgtaac tccgcctgat aagcagcttc
     6961 caactccgca cgttcgtttc taaactcgcg ttgctgttct tcttttgctt cttcaatttt
     7021 cttttgaagt tctgtttgat attctgacat ttcctgagtc agctgtttga tttgcttttt
     7081 ttgctgccat agtgttgccg aagcagtcaa aaatacaatc agtgcaccaa tcagcgcaga
     7141 accgatgatc actaagatca atggcgcaga aaaggaagtg aagccaaaac ttacaggaac
     7201 atttttattg ttcaatacag caaaaagaac aataatcaaa actaaaacaa atccaagaat
     7261 cacattgctt tgctttttca attccttcac ctactttttg ttaaaaattc cgctggctaa
     7321 ataatcacca atcgttggaa acagcgtata aaattttgat gcaacttcta aaataaacgg
     7381 ttgattgatt tcccgttttg gatgtctcat attgcggacg atcatttttg ccaacttggc
     7441 aggttctaaa acgatttgtc cgactttttc caaatagctt ccgcttggat cagccttatc
     7501 gaagaaattt gtctcgattg gacctggatt cactgtcgta acagctacgc caagtggttt
     7561 caattctaat cgcaaagcgt tggagaagcc aaggactgca aatttcgtgg cagaataaac
     7621 cgttgatttt gcagttgcca tttttccagc catagaggcg atattaatga tatgaccttg
     7681 tcttctttct gccatatcga tcgcaaattt ttgtgtcaaa accatcattc ctaagatgtt
     7741 gacttcaaac atatcgtatg cttttccaag gtcgaatgta aggaagtctt cgaaaatccc
     7801 aaaacccgca ttgtttacca gaatatcgac gcgcccgact tcttccatca ccttttcata
     7861 taattcgtcc acgctttcag gattgctgac gtctaccttg aaagcataag cctctttgcc
     7921 gctcaagcgt ctgcactcgt ctctgacacc ttctacaaat gccattcttc gtgcgcaact
     7981 tataacgatt gctccttgct ttgctgcttc ataacagatt tgtgcgccca gaccgcctga
     8041 actacctgtt actaaaacaa ctttatcttc taaattcatg cctcttcctc ctcgcgaaat
     8101 ggaatctcaa tgctatcaaa atctttcata atcgtcgtgt tttcaaacac ttcttgtgct
     8161 tctttttcaa gctctaaagc tgcttttcct aaatatctcg cactgatatg agtaagtagc
     8221 agccgttttg cctgcgcttc ttttgcaact tcagcagctt gatgagtcgt ggaatggaaa
     8281 tagttgtgcg ccattcttgc ttcatcttta ttaaatgtgc tttcatgtac cagaatatcg
     8341 ctattttccg caagcacaac ggaattatgt gtttttctcg tatcgcctaa aatagtcacg
     8401 attcgtcctt ttttatctgg acctacgaaa tcttttccat tgatcgtccg cccatcttct
     8461 agttggatcg tttcaccttg cttcagtttg ccgtacagtg gtccagctgg aatacctaat
     8521 tcttttagac gatccacctg caattctcct ttatgatcat gttcaaccac acgataacca
     8581 aaactgtcga tcccatgatt tagttttttt gtataaacag aaaattgctg atcagtaaaa
     8641 atcggggctg tttcatttag ctcaatgaat ttcagcggat aacttaaacg cgtttgcgaa
     8701 atacctaaag agacttttat gtattcctct atcccttttg gcccgtaaat ctctaatggc
     8761 gtatccccac cttgaaatga ccggctgctg atgagcccgg gaagtccaaa aatatgatcg
     8821 ccgtgtaaat gtgtaatgaa aattttaccg atttttctcg gacgaatagt cgttcgcaaa
     8881 atctgcattt gcgtgccttc gccacagtcg aacaaccaaa cttcattgcg ctcgtctaaa
     8941 agtttcaatg ctatacttgt cacatttcta tgttttgcag gaacacctgc acctgttcct
     9001 aaaaattgta attccataat ctacctgact ttctttaagt tcctctttaa ttttagggta
     9061 aaaagaaggg ataatcaacc gtaagtcgag aatgggttca aattaaaaat aacgttctaa
     9121 agcgatagcg ttttctttga aaattactat aaaaacagca gcgaacccgt ttgagatcct
     9181 ctgctgtttt ttattaattt gaagatccag aactttcatt gtcgttggaa gtttgcggat
     9241 gtttgtcctt ccaagtcttt tgtgcagctt ctttcgcatg attttcagct tctttcgcat
     9301 catgcacatc tttttcgaca tcttcataat ccaagcgagt aatttctccg cctaattcca
     9361 tcattaccaa ttgattcagt gggcgattgt cctcttcatc agcaatcaag atcagtcctg
     9421 tagctccttc accgatttta tttcctacaa agtcgaacac ggtctgggca tcttttattt
     9481 ctttggcatc ttgtgtcgca ccgatcattc ctccggcaaa ccatcctaat aaaatcccta
     9541 gcggaccacc taatacacca accaacattc cgattaatcc acccgtagaa gatttgttat
     9601 taccggtaaa gtcgataaaa tcttctattt tgaattgatg tgtcccatta ggatcatggc
     9661 tgacaactgc catctgttct cctttaatcg aacgaacagc atgtaacttt ttgatttctg
     9721 aaaatgcctg atatgcctgg ctttctactt gaaaatttaa aatgattact cgtttgctca
     9781 ttaaaaacgc ccctttcata ccttcttgtt atttatttta cctttaattg taaaataaga
     9841 aaagaaaagc actctttttt tccaataaaa tagggctgtg acaaaagtcg tatcacagct
     9901 cctttttccg aataaacggc gttcttgaag ctgatcactt ctgtcacaac ctcccttaaa
     9961 tctgaatcta tattttttgg tatttgtttt tatttgcttg cctattcgac aaattcaaat
    10021 tcaaactcac cgatacgtac aaggtcgcca tctttcgcac cgcgtgcccg aagagcttca
    10081 tccacaccca taccacgtaa ttgacgagca aaacgcatca ctgtttcatc atgatcgaaa
    10141 ttcgtcattt ggaataattt ttcgatcttc tcaccagata agatccacgt tgcatctgaa
    10201 tcacgatcga tttggaactc aggaccttct ggattgaatc catagtgtac agtttcttct
    10261 tccagttctt cttcgtacaa tgggaattct ggcgttactt ctaacaaatc tgctgtagca
    10321 ttcaataacg cgtccaatcc ttgtcgagtc actccagaaa ttggaaatac tgggatgtca
    10381 tcagcaaatt catcttcttt ttccttgttc agctgttctt tgaatttcac tagattttcc
    10441 ggtgcatccg gcatatccat tttgtttgcc acgatgattt gcggacgctc tagcaagcgt
    10501 aagttataag tagataattc tttgttgatg gcaagatagt cttcataagg atctcgtcct
    10561 tccatcccac tcatatcgat cacatgtaaa attactcgtg ttctttcgat atgacgaagg
    10621 aattgtgtac ccagcccaac accttgcgaa gctccttcaa tcaagcctgg caagtctgct
    10681 gcggcaaaac ttctgccatc acttgtagta accattccta gattaggtac gagcgtcgtg
    10741 aaatgataag cgccgatttt tggacgagca gatgaaataa cggatagtaa agtcgatttc
    10801 ccgacagatg gaaaacctac aagtccaacg tctgccaata ctttcaattc tagttcgatt
    10861 tttcgctctt gtcctggttc accgttttca gcaatttccg gtgcaggatt ccgaggagag
    10921 gcaaaacgga tattccctcg tccaccgcgt ccaccttttg ccacaactag tgtttgacca
    10981 ttttcaatta aatctcctaa aagagcacca gtctccgcat ctcggacagt ggttccttgt
    11041 ggtactttaa cataggtatc ttctgaacca cgaccgtgca tccctttact catcccattt
    11101 tcacctggct gcgctttgaa atggcgattg aaccgaaaat ccatcaatgt acgtaaccct
    11161 tcatcgacga tcaagatgac atcgccgcca cgaccaccgt ctccgccagc cggtccgccg
    11221 tcaggaacgt atttttcgcg acgaaatgca accattccgt ctccgccctt gccagctttc
    11281 acatcgatcg ttacttgatc taaaaacatg gacataatta agtcctccta ttattcattt
    11341 atttatttga ctgcctgaat ttccacaata aacaaaagtc ttacttattt tatcggtgaa
    11401 atctgatttt gtctatcatt tcagcaaaaa gattgtttac cttcttcttt ttttatcatt
    11461 tggtaaggtt tccaaccttt tttctgctac caagtataat tttttgacaa aatattcttt
    11521 gcgtagcaga ataacagtgt attaattgta ttaattgtat taattgagga tgccgtttca
    11581 cagcacccct aataaaggag gaacttttga tatgacagta aaagtaggta ttaacggatt
    11641 tgggcgtatt ggccgtctgg ctttccgccg catcaaagaa gtctcagatg atattgaagt
    11701 agtagcaatc aacgacttaa caagtccaac tatgttagca caattattgc aatttgactc
    11761 tactcatggt acttatcctg gcacagttac tgcaacagaa gactcaatcg tagtggatgg
    11821 cgaagcaact cgtgtttatg cagaacccga cgcaagcaaa atcccttggg caaaagaaaa
    11881 cggtgtagat atcgtactag aatgtactgg tttttataca tctgaagaaa aagcaaaagc
    11941 gcatttagat gctggtgtca aacgagtagt tatttctgcc ccagctggtg ctatgaaaac
    12001 catcgtctat aacgtcaatg atgatacatt agatgaaaac gacaaaatca tctcagcagg
    12061 ttcatgtaca acaaactgtt tagcacctat ggcttatttc ttaaataatg aatttgggat
    12121 cgaagttggt acaatgacaa cagttcatgc ctacacttcc acacaaatgc tcttagatgg
    12181 acctgtaaaa ggcggtaatt tgcgtgctgc tcgttcagct gctgacaata ctatcccaca
    12241 ttctactggt gctgcgaaag caatcggctt agttatccct gagctacaag gaaaattaca
    12301 aggacacgca caacgtgttc cagtcgttga tggttcatta actgaattgg tttcagtatt
    12361 gaaaacaaaa gtaactgctg atcaagtaaa cgaagcaatc aaaaaacaca cagttgacaa
    12421 tccttcattc gggtatgatg accgtcaaat cgtttctagc gatgtcattg gaacaactca
    12481 aggttcgatc tttgacccaa cgcaaacaga agtaacaact gctggtgatt tccaacttgt
    12541 taaaactgtt gcatggtatg acaatgagta tggatttact tgccaaatga ttcgcttact
    12601 agaaaaattc gctaacttgt aatttgatag atcaatcgat aaaagaagcc tttgactgga
    12661 tccagtcaaa ggcttctttc ttttcatccc ctacttttaa actcgttcaa tgaacatatt
    12721 ggcttaaaaa ttgttggact acttgctgat atttttcagg atctgtcttg tatgcttccg
    12781 catgctcggc tccttttaca acatactttt cttttggtcc cttcgttgca cgatagacct
    12841 tatctaacat ttcaaatggt acaaatgtat cttcgtctcc atgtataaag agcattgggc
    12901 gctggttttt cttcaattgt tccactgcat ttgcttcacc gaaaaagtag ccagcacgga
    12961 cttttgtcac tagactcgtt acttgcataa gcggaaatgc tggaagacta aacatatcgt
    13021 ctagctgata agcaagctca tcatgaacgg aagaataacc gcaatcttca atgattgctt
    13081 tcacattatc tggtagtttt tctccgctag tcatcataac tgttgctgct cccatgctga
    13141 caccgtatag aacgatttct tgcgattttc cattgttttc tagcactttg tttatccact
    13201 gtacatagtc tttacgctct ggccatccaa atccaatgta atccccttcg ctttttccat
    13261 gtcctcttgc atcaggaaca aggacattgt aacccatatc atgaaacatc tttgcgtaat
    13321 tggtcatcgt ttcagcgttt cccatatagc catgagccac aagagctgtt ttttcacttt
    13381 tcgtttcagc aggaagataa atcgctgata atttcagacc gtcttttgaa gtgatggtcc
    13441 aatcgcttcg gttttctttt gaactaaacc atttatgtgc atccgtttct ttggatgtct
    13501 gtgttgtacc aggtgtatct tcgtctaaaa agtctttctc agaaggcaca acagcataat
    13561 tataaaaata attccctgca aacactaacg caacaatcag agctaaagcg acgcctaaaa
    13621 ggattttcat ccaaagtttc ataaatcatc catctcattt ctcttttttc atataacctt
    13681 gaattagtat aatcaaagaa tctctactat gcaatgttca tttttataac
//
LOCUS       DACDYM010000072        13701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDYM010000072 DACDYM010000000
VERSION     DACDYM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.072"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..902)
                     /locus_tag="IWM59_002120"
     CDS             complement(<1..902)
                     /locus_tag="IWM59_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9980139.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IWM59_002121"
     CDS             1185..2153
                     /locus_tag="IWM59_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9980140.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IWM59_002122"
     CDS             2266..2544
                     /locus_tag="IWM59_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9980141.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="IWM59_002123"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="IWM59_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9980142.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3010..3756
                     /locus_tag="IWM59_002124"
     CDS             3010..3756
                     /locus_tag="IWM59_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9980143.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="IWM59_002125"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="IWM59_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP9980144.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="IWM59_002126"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="IWM59_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP9980145.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="IWM59_002127"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="IWM59_002127"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9980146.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="IWM59_002128"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="IWM59_002128"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9980147.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="IWM59_002129"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="IWM59_002129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9980148.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="IWM59_002130"
     CDS             8493..9035
                     /locus_tag="IWM59_002130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9980149.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="IWM59_002131"
     CDS             9058..10614
                     /locus_tag="IWM59_002131"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9980150.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="IWM59_002132"
     CDS             10628..11530
                     /locus_tag="IWM59_002132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9980151.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="IWM59_002133"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="IWM59_002133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9980152.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="IWM59_002134"
     CDS             12973..13395
                     /locus_tag="IWM59_002134"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9980153.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2451 c         2988 g         3732 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag a
//
LOCUS       DACDYM010000073        13146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDYM010000073 DACDYM010000000
VERSION     DACDYM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.073"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(77..149)
                     /locus_tag="IWM59_002135"
     tRNA            complement(77..149)
                     /locus_tag="IWM59_002135"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IWM59_002136"
     CDS             530..1099
                     /locus_tag="IWM59_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9980154.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IWM59_002137"
     CDS             1086..1946
                     /locus_tag="IWM59_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9980155.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IWM59_002138"
     CDS             2136..2840
                     /locus_tag="IWM59_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9980156.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IWM59_002139"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IWM59_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9980157.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IWM59_002140"
     CDS             4677..5993
                     /locus_tag="IWM59_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980158.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IWM59_002141"
     CDS             5994..6863
                     /locus_tag="IWM59_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980159.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IWM59_002142"
     CDS             6918..7727
                     /locus_tag="IWM59_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9980160.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IWM59_002143"
     CDS             complement(7830..8459)
                     /locus_tag="IWM59_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP9980161.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IWM59_002144"
     CDS             8629..9921
                     /locus_tag="IWM59_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9980162.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IWM59_002145"
     CDS             complement(9997..10659)
                     /locus_tag="IWM59_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9980163.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IWM59_002146"
     CDS             10884..11168
                     /locus_tag="IWM59_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9980164.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IWM59_002147"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IWM59_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9980165.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattc
//
LOCUS       DACDYM010000074        13084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDYM010000074 DACDYM010000000
VERSION     DACDYM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.074"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(78..2198)
                     /locus_tag="IWM59_002148"
     CDS             complement(78..2198)
                     /locus_tag="IWM59_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9980166.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="IWM59_002149"
     CDS             2428..3606
                     /locus_tag="IWM59_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9980167.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="IWM59_002150"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="IWM59_002150"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9980168.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="IWM59_002151"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="IWM59_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9980169.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="IWM59_002152"
     CDS             complement(4959..6212)
                     /locus_tag="IWM59_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9980170.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="IWM59_002153"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="IWM59_002153"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9980171.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="IWM59_002154"
     CDS             7789..9135
                     /locus_tag="IWM59_002154"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9980172.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="IWM59_002155"
     CDS             complement(9443..9829)
                     /locus_tag="IWM59_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9980173.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            10708..10920
                     /locus_tag="IWM59_002156"
     CDS             10708..10920
                     /locus_tag="IWM59_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980174.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="IWM59_002157"
     CDS             complement(11198..12997)
                     /locus_tag="IWM59_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9980175.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 ttaa
//
LOCUS       DACDYM010000075        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDYM010000075 DACDYM010000000
VERSION     DACDYM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.075"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWM59_002158"
     CDS             6..305
                     /locus_tag="IWM59_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9980176.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWM59_002159"
     CDS             378..1739
                     /locus_tag="IWM59_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9980177.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWM59_002160"
     CDS             1761..2021
                     /locus_tag="IWM59_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980178.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWM59_002161"
     CDS             2035..2787
                     /locus_tag="IWM59_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9980179.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWM59_002162"
     CDS             complement(2881..3486)
                     /locus_tag="IWM59_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9980180.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWM59_002163"
     CDS             complement(3539..4513)
                     /locus_tag="IWM59_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9980181.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWM59_002164"
     CDS             complement(4891..5670)
                     /locus_tag="IWM59_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9980182.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWM59_002165"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWM59_002165"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9980183.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWM59_002166"
     CDS             7161..8558
                     /locus_tag="IWM59_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9980184.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWM59_002167"
     CDS             8639..9079
                     /locus_tag="IWM59_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9980185.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWM59_002168"
     CDS             9227..9553
                     /locus_tag="IWM59_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9980186.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWM59_002169"
     CDS             9554..10303
                     /locus_tag="IWM59_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980187.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWM59_002170"
     CDS             complement(10383..10838)
                     /locus_tag="IWM59_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9980188.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWM59_002171"
     CDS             complement(11265..11540)
                     /locus_tag="IWM59_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980189.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWM59_002172"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWM59_002172"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9980190.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2174 c         2587 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt cattccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACDYM010000076        12169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDYM010000076 DACDYM010000000
VERSION     DACDYM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.077"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            335..1246
                     /locus_tag="IWM59_002173"
     CDS             335..1246
                     /locus_tag="IWM59_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9980191.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1463..1900
                     /locus_tag="IWM59_002174"
     CDS             1463..1900
                     /locus_tag="IWM59_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980192.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            1995..2879
                     /locus_tag="IWM59_002175"
     CDS             1995..2879
                     /locus_tag="IWM59_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9980193.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3103..6270
                     /locus_tag="IWM59_002176"
     CDS             3103..6270
                     /locus_tag="IWM59_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980194.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6290..6835
                     /locus_tag="IWM59_002177"
     CDS             6290..6835
                     /locus_tag="IWM59_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP9980195.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            6863..7318
                     /locus_tag="IWM59_002178"
     CDS             6863..7318
                     /locus_tag="IWM59_002178"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP9980196.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            7315..8073
                     /locus_tag="IWM59_002179"
     CDS             7315..8073
                     /locus_tag="IWM59_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9980197.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8159..8899)
                     /locus_tag="IWM59_002180"
     CDS             complement(8159..8899)
                     /locus_tag="IWM59_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9980198.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            8960..10213
                     /locus_tag="IWM59_002181"
     CDS             8960..10213
                     /locus_tag="IWM59_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9980199.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            10535..10762
                     /locus_tag="IWM59_002182"
     CDS             10535..10762
                     /locus_tag="IWM59_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9980200.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10885..11211
                     /locus_tag="IWM59_002183"
     CDS             10885..11211
                     /locus_tag="IWM59_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9980201.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11279..11992
                     /locus_tag="IWM59_002184"
     CDS             11279..11992
                     /locus_tag="IWM59_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9980202.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         4218 a         2020 c         2514 g         3417 t
ORIGIN      
        1 aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccag cagcggaaga
       61 agaaaaacaa aatcgataac atttgttcta agaattagtt acgttaaata cggaacgcaa
      121 gatagacttg aaaaacggta acaaaagaag tagtatatta aggtgtgatg tgctacttga
      181 catttattct gctgagcgaa ctttctggat tgtgtagcct tatatctatt caggacgtat
      241 cgagcagatt agtaaaggtg ttcttgatga ttttcatctt gaacaccttt gtttttagca
      301 attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa aaaagatttt tagcacttgc
      361 catcgtacta ggcactggct tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt
      421 tagttataaa ggcggtacaa tcagcgaaca agaagtaatg gatagcttga aaaaaatgca
      481 aggagcagat tccgctgtgc aacagttgat cgtttatcaa gtatttgaag ataaatatgg
      541 agatgacgta tcaaccaaag aaatcgattc tcaatatgat caaacaaaga aacaattggg
      601 tgattcattt gattcacaat tgaaatctgc aggttacaca gaacaaacat tcaaagacag
      661 tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa catatcaaat tgaccgatga
      721 agatttaaaa acggcttggg aatctttcca tccagaagtg gaagctcaaa ttatccaagt
      781 agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct gataagggag acgatttctc
      841 taaattagcc aaagacaagt caactgatac tacgacaaaa gaagatggcg gaaaagtgaa
      901 atttgattcc actactacaa cggttccggc agaagtcaaa gaagcagcct ttaagttgaa
      961 agatggtcaa gtttctgatg tgatcacttc taccaatgct tcaacttata ctacggaata
     1021 ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac gacatggata aatacaaaaa
     1081 agaattgaaa gaaattgcaa cagacacaaa attaagtgac agtaccttcc agaataaagt
     1141 aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt
     1201 tttgagtaca ttcacttcag attctagtac agcaagtagt aaataagcgg tataaataaa
     1261 aaatatgaac tatttaaatc aataaaactg ttcatctaaa tctttttatt agaactaaat
     1321 caacagtagt ttaattacta cttgatttag ttctttttat tttaaaaatt ttttttactt
     1381 agaatccagc gaccctagat atttttctgt ataatcttaa ataaagaaaa caatatcaaa
     1441 aaatgcacgt agaagtagat aaatggagtg gttttgcaaa tttcttttat ttctagaagt
     1501 tattcacgta tacggatttg tttttctcac aaataaaaag gcaggaagta caatggatca
     1561 tttgtatata aaatatattc atcaattttc cgttttggtg ttaggcacac taattatagc
     1621 ggggattgtc gcatttatta ttcaaggccg tgacatatta acagtacatc aagagttttt
     1681 cagttatgca tttggtattc ctctgatcgc tataaggtct gataacaaaa agtggaagaa
     1741 acgctattac attctcttgc tatttttgtg tttttatctg ccacctaaga tttttacttt
     1801 attaggtata caatgcctat cattttcttt tatttcctat atcattgttt taattttcct
     1861 cagtttcata gtatggatgt tttatcttgt caaaaaataa atgaatgttt catgaacagt
     1921 acttcatcac agcctaattt ttttctttat gctatcgtta tattaaagaa atcaatcaga
     1981 agggaaagaa agtgatgaaa aaagttttat tagtggtaaa ccccagttca ggcggtgaac
     2041 aagcaaagga atttgagcaa ttagcgatag caaaattgga atcggtgttt gatgaagtgg
     2101 tcgtcttgca tacaaaaaaa gcaggggatg caaaaaactt tactcgcgaa gcggctacgg
     2161 aagggtatca tagtgtgttt gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg
     2221 gaatcgctga acaagaacat cggccaaatt tcggattttt cccattgggt acggtgaatg
     2281 acttggctcg agcattaggg ataccgctag aacctgagga agcgatcaat catttttcga
     2341 ttgaatcagt caagccattg gatattggaa aaataaatga cgactatttt atgaatgtcg
     2401 tcgcaattgg ctctatccct gaatcaatca atgatgtaga tgcggagaag aagacgaaat
     2461 ttggaaaact agcttatttc atgtcaggaa tcaaacagtt agcttccaca caatcctact
     2521 cgtttcacgt agaagttgat ggaaagaagg aagaaattga gagcagtacc ttgctgatag
     2581 gactaactaa ctcagtcggc ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg
     2641 gtaagctcca tttggtttat ttaaaagatt cttctttgat cgatacgctt aaaacattgc
     2701 cagacctatt aaaaggagta gatgaatcta cgaaaaatct agcctacctt acttgtgagc
     2761 aattagttgt gtcattagct gatgaggaag aattgacgac gaatgttgat ggagatgaag
     2821 gggataaatt acctgtgaca atcaaagtat tgccctcaca tttacaggtt tattgttaaa
     2881 atggaggttg tgaccgagga gttggcggga gaacaccgct tgttcgggat aagggactgt
     2941 gacaagactt ttgtcacagc cttttttttg tacaaaaaag aaatttacac ctaatagaaa
     3001 aatttttcca taaaaataca tattttaaat atgaaagtac aattggaaat agattaaaac
     3061 gataaaataa aattgtcaaa acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa
     3121 aagctgattg cgtcgatttt gtttgtcttc cctctcttga tggtgacatc cgtttttgcc
     3181 gatgtagaga aaacaaacga atcaacgcaa tcagagaatc aaagttttga caagactact
     3241 ttttcagaag aatcgattac ttctgaaact acagaatctc aaagtgaggc ttctactaat
     3301 aaaaggcaag aagaaaataa gacaagtgag acctcagaag tagctgaaca aacgtctgat
     3361 agcagtgaag cggcactggc aagagctgtt tcagcggatg ggtacagcga agctgccaca
     3421 acagaagaac tggcgcagct tctcgcagac gaatcagtaa ctaagatccg actgattcag
     3481 cctttgactt tagacaggga attagagatc aaacgagata tcgttattga ttttggtgga
     3541 ttcgctcaca atttcggcac acaccatatt tatatcaatg agatacctca gtcgatcgag
     3601 ttccagaatt ttaaaggaac agctgcacat ccaggtggat tgacaccaaa tatcgatgga
     3661 aatgccatta tcattgctta tatgaccgat ttttggggaa gcagatatca ttttacggga
     3721 gaaataaaat ttaccgggac acttgatttg tatgatgggt ccaaattagg actgatttac
     3781 gctcctcgtg caactgtcac attagatggt gtttccggtg tgttggatgt ccaaccagtg
     3841 aaggaaggac tgaatgccgc tccgggtaaa gcctattttt gtagaagcta tcaattgaat
     3901 gtgatcaatg gtagtcaact ttatggaccg tatctaggga aattctacgg ttttctagat
     3961 tcaggagatg agactggtag tggaaaaaca gcgccaggaa tccatatctt aagcggttca
     4021 aaggtctctc ttgattatga tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat
     4081 acgctacctg gtaatgtcat gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat
     4141 acaaagatca atattaccaa tgacaacaat cgcggaatca tccaaatgag aggttcagga
     4201 tcgaaagtca ctgtatcgaa tggtgggaaa atcaccatca atacagcaac tacgggcgga
     4261 tttcgcttgc aaggagattc tagtcaaatc catgtatcaa gtggtggaca aatctcggtc
     4321 aaaatggctg gagatgacga tcaacccggg aacaatggga tgcgttttgt aggaaaaggc
     4381 ctttctttga tcgtagaagg agccggatca gttatcgaca tcgataagca gagtgggcgt
     4441 tccagcgcag ttcgttttga gaatggtacg caaaagctga cagtaaccaa tggaggctca
     4501 ttgagggtaa gaaatacagg tgatggcaaa tcttacgtga acagactcaa tcaaggaaat
     4561 caagcgatcc aattttatga cgcttctggt tttctagaaa gtccaggttc agctgatttt
     4621 acagtcactg gagaaaaatc aaatatcgat atacgcgcag attccggagc aactgtagat
     4681 acaactggag atattgattt aaattttacg gcaggtgaaa aaacttattt agtcatggtc
     4741 gggaaaacag gggaagataa gaccggcatc atcagtgggg atgtgttgaa tgtggactta
     4801 gaatctccta cctattttga tttccaaaac aaacggattg gaagcgagac caatggcata
     4861 ggcggctggg tattccaagg aaatggagat tcttctttaa agatcgccca ttcagaaatc
     4921 gctttatggc gaaaaacagg cgcaacattc gataactttg aaaaagaccc ggattatttt
     4981 tctgatatga cagacttatc gatcaatggg accgacttag gcaatttcgt atcaagccct
     5041 agtgcaggtt taacaaatga atttgctcag acaggtgccg gaatgaaaaa ttataatcga
     5101 atcagtgcca ataatcagct cccgatgatt gaatccttaa gagtgccaac gaatgcagat
     5161 aaaaaaatct atgggcatgt cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct
     5221 tggacaggcg aagtagaagt gactttacgt attacttacg cagatacgac gaaatcatct
     5281 gtagaaatta ctgctctcac gcaaggacaa acagatgaaa atgaaggggc aggatataac
     5341 ccatggggag aaggcgaaca aggcggcttg ttcgaagttc aagtacccaa tgatgagaga
     5401 ttaaaaacgg aggatcaagt gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa
     5461 gtgacagaac tggactctgc caaaacgaca gtcgatgtga ttcctcctga acctgctaat
     5521 ttaagtacca cattgatcaa tgcagcttct cgcacgatca caggtacagg tgaagcagga
     5581 gccttggttt cattgaaaaa aaatcaagct gttttgtcag aaacaactat tgatgataca
     5641 ggggaatttg ttctttctat cccagaaaat caactttttg caggagatgt attagaaatt
     5701 gtattaaacg accaagcagg agaagctact gctaagggtg tgcagaacaa gccaagtacc
     5761 aacgatgaaa ttgggaatca caatccaagt gatacgccag ttgtttatca tgatgccccg
     5821 ccttttgcac ctgctacgaa gatcgtagta gaaggtggtc tctcatttct tgccccttcc
     5881 aaactcgatt ttggtcagat caaagcaact ggtttgacac aagaagcatt tggccaaatg
     5941 gatatacagg aaaaattatg gatttatgat cagcgagaaa cgaaaaatcc gtggatctta
     6001 acgataaaat tatctcagcc tttcacaaat gatgcaggat tttctatgca ggatgtccta
     6061 tatttcaaga aaggagagga gtatcaatta gtgaatgaag aagaactgga aattgtcaga
     6121 ggacaaaatt caacgaatgt gcaagcagat tattcagaat atttaaataa cggtcgggcg
     6181 tttaaacttg tcttaaccaa agaaaatcag atagctggta attactcagc agagataaca
     6241 tggacattag cagatgcgcc gacagaataa agatcaagga ggatgattga tgaatcgaat
     6301 atggaataaa agtcatcgaa acaactgctc tttacttatg atccttctcc ttggattcat
     6361 cctttggcag acacctgtcc atgcctccga attcaatttt gcggtagatt ttgaattacc
     6421 ggctaatcag attgataaga acaaaacata tataaatgta aggatggagc ctgggaaaac
     6481 acaggaaatc ccctacacgg tctttaaccg aacagataaa tcagtaacag ttgagacatt
     6541 gatccactcc gctacgacga attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa
     6601 atttgatgaa acattaagtt atgatatgaa aaacttagca aaaacagtgg gagaaataac
     6661 tgttcctcct caaagtgcgg tcactgagac actttcactg aaagcgccag aaaaagagct
     6721 ggctggtgtg gtcgcaggag ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa
     6781 aagtacagtt ggtgtcgtca atcagttttc tcaagtgaaa gcaatcttga tttgaaatag
     6841 cgaaaaagag tcttccccag atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg
     6901 aagaaatgtg attaaggcga atcttcaaaa tccaaatgct tcttatatgt tcggcttgac
     6961 tacatcggtt gaaatctaca aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga
     7021 aaaaatagat gtagcaccca atacaaattt tgatttactt gtcccattag aaggaaaaaa
     7081 attagctccg ggacattatc aagcggttgt gaagggtgct tggaaagaaa aacgctggaa
     7141 tctccatacc gattttgaaa taactgatga tgcagcggac caattgaatg aaaaggatat
     7201 tgatttaaaa aatcagccag aaacatttga ctggcgatgg ctgatacttg ttggagtcat
     7261 tcttattggc ttatttgctg gattatttat tttaaaagaa agaaggaaaa agaaatgaaa
     7321 aaaacaattg cattaagtac cgttctagct ggaagtctat tattatgtac acaaagtgtt
     7381 tttgctgaga ataagagctc agccactaca gaaggaacga tttctgtgac caaaccagat
     7441 gctgcaaatc ctgaagacgt tgttccagtt cctcctgttg atccaaatga tcctgatact
     7501 ccttttacac ctgacgaacc aaatcaggga acatccggat tactgacgat tgaccaagct
     7561 ccagatcttg attttggaac aatcaagtta ggtacagcaa aaacagtata tgctgcatta
     7621 caaagcggaa cggacagtac tggagcacaa acacaagtac agaatttcgt acaagtaacg
     7681 gataaaagcg gaaattatgc cggttggaaa ttatcagtca aacgaacaga atttattggt
     7741 acaacatctg atacgaataa actaactggt tcaaaggtga aattcaaaaa cgccgtgatt
     7801 cgaaatagcg caactaatag cagtggaatg ccatctaccg tttctggaac tggcgctgaa
     7861 ggaatcgaaa tcccagcaga tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg
     7921 attggcacat ggatttattc attaggagag aatgtggaac aagggagaca atcagtggaa
     7981 ttatttgttc ctgtatccaa ttatgctgta gacaactacg tttcaacatt cacatggtcg
     8041 ttaacagatg ctcctacaga agaggaagcg taaaatcata tcaacgattt cagggagtgg
     8101 agccgattca aggcccactc cctgaaatct gctatgctta cgtctcagct cttattcatc
     8161 aactcgaagc agttttttgg aagtcatgga aacgttcctt tgtcttttca aagatctttc
     8221 gaccaacgag taaggagtca ccgttgaaaa attctaacac ataatgagca gaatcaattt
     8281 tctgcaattg cgataagttg atacagtaag attttaaaag taaaaagtaa gcatactttt
     8341 ctaactctgt cttcaattgt tttaaagaag tattcactaa tttatcttct gtagttgttt
     8401 tgaccataac ttggtttttc aaacctttga tactttcaat atataaaatt tcccgaggat
     8461 acaggacgag acttccacct ttttgatcaa taaagatttt atcgacattt acgatctgct
     8521 gttctttcca attgatctgt tctagcactt gacgaatttg ttcagataaa ttcctgtctt
     8581 ttctgatata tccaatcgga aggattttcc gattgatgat ctctactgca aaattcgtat
     8641 aagcggtgaa aaaaacaata ttactatgtg catttttttt acgtatttgt tcggcgacat
     8701 caatacctgt gagtgattgc ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa
     8761 tagtgcccaa ctggatcaac tgattgattt gagagataga agtgagctgc acgatattct
     8821 cgatatgatg attgaccaat tctctgatga tccattcgct gtgaaactgg ttatcttcta
     8881 tgataaaata ctttttcata tgcaggctcc tattaaaaaa atatgtgtac attttgaaac
     8941 aaaacaatgg aggaataaga tggtcgcaac agtgacactt tatttgacct ttactttgat
     9001 tttagattta ggtatctatt tattcttttc gaaacaactg ctagtaaaaa acagctttgg
     9061 ttgtatagct ttgatggtgg tgacactttt acttcctatc cctaaagtga gctttcctta
     9121 cctagttggc ttattattga tgtttctttt ttattttact acgaaaaacc tgtttatcag
     9181 ctgtttgatc agtaatcttt tttttgctat cactggactc agcttttttc tggtttttga
     9241 tctgatggat tatttaggaa agagacatct tggaacagca agtttttttg cactacttat
     9301 cgcatttatt ttctatctat tagtaaaaag tatagacaaa cagacgagat ttctcaactt
     9361 gctttttact tatccaactg aacactattt tggaggtatt tgtattttat taagctgggg
     9421 cgtgatttac ttggtattgg gaagtgttca cttccgctca tctaattatc tgcttctatc
     9481 tcttttgtcg atagtcatca gttttttttc tttgtttttt tccattatgt tgatcagttt
     9541 ccaagtaaaa gaaagacaac atcaggaatc gcttcaactg tatcaaataa atgaagaata
     9601 ttaccatcat ctagaggaat tcaaacatga ttataaaagt ctactgtttt cattaaaaat
     9661 tactcttcag gaaaataaaa aagaaacgtt ggaattttta gaaatattag aagatcattc
     9721 tgcagctatt tttgaaaaac cgcaaataca acagctaaca aatattgtat ccccagctgt
     9781 caggggtgtc tttttaaaat ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt
     9841 agttattcct gtcgaagtga aaacaatccc gattgatctg gttgttttta ttcgctgttt
     9901 atctattttt atttctaacg cattcgatca tcgtgtagcc gatcagagtc cgattacgat
     9961 caatctgctg gaagaaaaag agggatcacg ttttagtata gcgaacaagg caaatccaac
    10021 gaatttgacg ctatcggaga tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt
    10081 aggtctatac agtgcgaaaa aaatgttgga tgtttacgaa aatgcagaac taaaaatcga
    10141 attttcaaaa cagaatcact atttttttgt agaaatgtat ctggcatcta gaaagagcaa
    10201 atcagcctac tgacttactg tcaaaggata agtttgagga aagtctacac ggagatagag
    10261 acaacaacac agcatattca aaagaaacag aagcgtgatg accgttgtca cccttctgtt
    10321 tcttttgcta tgtagttaaa caaagtagtt gacacttccg ctacttagta ttagtacgaa
    10381 agtatacttc atgatactaa gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa
    10441 tgctaactgc tttgcttaca ccagataaag ggaagagcaa tatcgcttat tttaggcgtg
    10501 gagaagcaag caaatcccta cgatcacaaa aaaaatgctg acccagcagt taagagagtt
    10561 agaacaagat gaaagtatcc atcgaaaagc ctataaccaa gtcccgccta aagtagagta
    10621 ctcgctcacc gaagaaggaa aatcattaag agagattcta gtagcgttgt ctgtttggga
    10681 agaagaacgt atcgaaaaac aaaaagcctc aagggaagaa gacacgctac tgcatacaca
    10741 tggtggctac ctaaattatt gattttctat gacatttatt ttaaataaaa gaaaacagga
    10801 gtttttttaa aagcatcatc tattttttat gtaaaaaaat ggtatgatta atcaagtaag
    10861 aagtgattgg aagaaggcaa gcaaatgaat agtatggaaa aagtcaacca gtttagagat
    10921 gaacgtaatt ggcgacaatt tcataacgaa aaagatttgg ctatctctat ttctttggaa
    10981 gcatctgaat tgttagaact ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa
    11041 ttagaacgaa tcaaagaaga actggcagat gtattgatct attcatacat gatggcagac
    11101 aaccttcacc tggatctaga tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa
    11161 tatcctgtag aaaaaagcgc aggtgtacga aaaaaataca cagagttgta gaaatgtgtc
    11221 attcggctgt tggaaagagc aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat
    11281 gaaaaaaaaa ggaaaagcaa aaagatggct agtcaatatc ctcttgttcc tcttgttatt
    11341 agtaggacta gctttgattt tcaatgaaca gatcaaggat tattttgtca gagagacagg
    11401 agataaatat gctatagcga atgtaacaaa agaagatctg aaaaagaata acgataaaga
    11461 tgtcagtttt gactttgatg cggtagaacc aatgacgaca gaaggggtca tgaggtcgca
    11521 aatgagagga acagatctac cagtgattgc tagtatcgca gtcccttcag tttcaatcaa
    11581 tttaccggtt ttcaaaggct tggataatac gtcactactt tacggagcag gaacattatc
    11641 tccagatcaa gaaatgggaa aaggaaatta tgccttagca agccatcgtg caacgaaccc
    11701 tgaattgctg tttacaccgc tagaaaattt agagatgggt gccaagatct atttgacaga
    11761 tttagaaaat gtgtatacat acaaaacatt ttttaaagaa aaagttgctc ctacagatac
    11821 ccaattactg aatgaagtcg agggaaaaga aattgtcaca ctaattactt gtggcgacat
    11881 ggacgccgtc acgcgactag ttgtacaagg agaactggaa agtgttacat cgataaaaga
    11941 tgcgacagat gatatgagat ctgctttcaa tctagagaca aaaacatttt aggatactgg
    12001 gacataagtc gttacagatt aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa
    12061 tttgctgaaa aaaagagaaa cgcaaagagg tgcatctctg atttggttgt tttctagata
    12121 gacttgtgtt ccagtttttt tattttttat atgaaaaggc ttgcataaa
//
LOCUS       DACDYM010000077        11397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDYM010000077 DACDYM010000000
VERSION     DACDYM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.078"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(369..1487)
                     /locus_tag="IWM59_002185"
     CDS             complement(369..1487)
                     /locus_tag="IWM59_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980203.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1958..3265
                     /locus_tag="IWM59_002186"
     CDS             1958..3265
                     /locus_tag="IWM59_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9980204.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3384..4583
                     /locus_tag="IWM59_002187"
     CDS             3384..4583
                     /locus_tag="IWM59_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9980205.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4610..5878)
                     /locus_tag="IWM59_002188"
     CDS             complement(4610..5878)
                     /locus_tag="IWM59_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9980206.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6041..6687)
                     /locus_tag="IWM59_002189"
                     /pseudo
     CDS             complement(6041..6687)
                     /locus_tag="IWM59_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7095..7598)
                     /locus_tag="IWM59_002190"
     CDS             complement(7095..7598)
                     /locus_tag="IWM59_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9980207.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7721..8485)
                     /locus_tag="IWM59_002191"
     CDS             complement(7721..8485)
                     /locus_tag="IWM59_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9980208.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8593..8868
                     /locus_tag="IWM59_002192"
     CDS             8593..8868
                     /locus_tag="IWM59_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9980209.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9306..10256
                     /gene="yidC"
                     /locus_tag="IWM59_002193"
     CDS             9306..10256
                     /gene="yidC"
                     /locus_tag="IWM59_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9980210.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10435..10635)
                     /locus_tag="IWM59_002194"
     CDS             complement(10435..10635)
                     /locus_tag="IWM59_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980211.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3427 a         2393 c         2011 g         3566 t
ORIGIN      
        1 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
       61 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
      121 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
      181 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
      241 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggaaaa
      301 atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac
      361 aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc atctttttga
      421 tccaaaataa acactttccc gccattaatg atcacatcat ctgctaatgc attcaacact
      481 tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta
      541 gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
      601 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
      661 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
      721 gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga
      781 ttttctggta atgcaaataa ataaatcggc cattgttcag gattttcgaa agaatccttc
      841 aagtacgtat caacagcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
      901 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
      961 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct
     1021 tctggtagag atacttcttt tacttgtttg ttgctgacct catacaattt catggagtcg
     1081 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
     1141 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
     1201 taggtctggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
     1261 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcggaatat
     1321 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt
     1381 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg
     1441 ccttctgaag tcaattgtgt caataattct ttatcctttt ttgccataaa cgtacccctc
     1501 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
     1561 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
     1621 agttatcttc ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat
     1681 gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat tatgattttc
     1741 agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa
     1801 acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaacttccat aacgcgcgtt
     1861 cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat aaaattttaa
     1921 tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa aaacacgtct
     1981 agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag ctgttgctgt
     2041 gcaagctgaa gatacgttca aagtaaatgc aaaagcagct tttgctgtag atgcgcaaag
     2101 cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt caatcacaaa
     2161 aatcatcggt ctttacatcg tccttgatca agtaaaagaa ggaaaactgt catggggtga
     2221 taaagtttcg atttcagatt atgcagaaaa tttgagtatc accccagatt tatcgaatgt
     2281 tcctttgcat aaagaaaaca cctacactgt caaagaatta ttcgactctg caatcatcca
     2341 gtcagccaat gcttcaatgg tggccttggc agaaaaaatc tctggcagcg aagctaaatt
     2401 cactgaacga atgaaagaac aattgaaaga ttggggaatc aaagatgcaa caatcgttaa
     2461 tgcatcagga ttgaacaact cttatcttgg cgaaaaccga ccagaaggca ctggagaaaa
     2521 cgacgaaaat caaatgtctg ctcaagatgt ggccatcgtt gcccgccatt tgatcttgga
     2581 tttcccagaa atactggatg tatcaagtac tacgacccaa atgtttggcg aaaatacgca
     2641 atcaccagtg gaaatggtca actggaactg gatgctccca ggattcgtta attacaagga
     2701 aggcgtggac ggattaaaaa ctggtacgac tgagcttgca ggtgcttgtt tcgttggtac
     2761 gatcactaaa aacgatcaac ggatcatcac tgtcgtattg aatgcggacg gccatgcgga
     2821 aaacccgagt gcccgcttca atgaaacaga ccgtttgatg gactactgct atgacaattg
     2881 gtcagaaaaa gagctcggaa aagctaacgc aagtattcct agtctaaaaa ccattgatgt
     2941 aaaagacgga aaagaatcaa cagttcctgt tgtgcttaaa agccctgtaa aagtatgggt
     3001 acgaaacgat atggatactg gcaaattaac gatcacgcca acacttgaca agaagcaagt
     3061 cactgacaat gagatccaag cacctatgga taaaggtaca aaaatcggta ccgctacgat
     3121 caccttggca gacgataaat taggctattt agaagacaac aatgcaccat caacagagat
     3181 cattacggat aaaacagtgg aaaaagcgaa tatattcgtg attggctggc gacgcgtcgc
     3241 agaatttttc ggcaacttgt tttaattgaa caataagaaa aacatctttc catcggaaag
     3301 atgttttttt gctttttcta gtttaaataa cagctttttt catctatttt cgttatactg
     3361 tataagaaaa aggagttgaa tttatgaatc taaccagaga tctgatcttt ttagttctct
     3421 atcttattgg ggtattctct atcgtttatt ggcgaaaaca gaagaaggat ccttccttgt
     3481 tcttttacgg cagtacgtgg acatttacgg taatcttaac ttattttgtc gtgttggaaa
     3541 ttttttattc gaccttttat tttttgattc cattgttgtt ctttagtatc tttgcttttt
     3601 cttatttcac agaaaaacga aggctactga atggtctttt attcaatgtt ttactcatca
     3661 gtttcggcat ctacttgttt gtcttgctgt atgaaacgca aaatattttt cttggcggac
     3721 tgatcgcctt gatcacgatt ccattgctac ttgtcctttt atttggtatt tatggattga
     3781 ttgtttttct tttttggaac ggggtcacag ttttacgtag agaatcgcat tcattagcta
     3841 atcttttaac gttgatacta gccatttttc ttacactgtt tcttgttttt gactttttct
     3901 tgttaaaata tttgccgcaa tggataaatg ctttgttttt ctgcgttccc ctgatcttga
     3961 tctatctatt tatcgtattc tacaactttt tgactgtctc attcttgtat cagttcaacc
     4021 ggcctcgata caatcaagac ttcatcgttg tgttaggcgc tggattgatc aatggagaaa
     4081 cagtttctcc tcttctagcc aagcgaatca ataaggcaat tgctttttat cgggctcaaa
     4141 gccgtgctac attaaatccg cccatcttat tgatgtctgg cgggcaagga gcagacgaaa
     4201 aagtaccaga ggccattgcg atgaaacaat atgccatgga acaaggaatc cctgaaagag
     4261 acattcttgt cgaaaccaat tcgacaacca ctttggaaaa catgctctat tcaaaggaaa
     4321 tcatggatca gcaaatgaaa gggccatacc gcgcgatctt tagttccaac aattaccaca
     4381 tttttagagc cggtctttat gcgcggcaag ccaaattgaa agcaaatggt attggtgcaa
     4441 aaacagcttt ctactacttg cctaatgcgt ttttaagaga atacctcgcc atcttgatga
     4501 tgaacaaaaa aagacatatg atcatttgcg gattgctttt cgcaggctcc gcattcttat
     4561 cgctgattac tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt
     4621 ttttttcttt cattctctta actattggca aaaagagtat cccttttttg accgctactt
     4681 gctgagcaga ataaataccg ctgttgcctg aacacataaa gctgatcaag cagatcataa
     4741 agaagtatac tgccgcttgt gaaccgaaca attcgatccc catcacaaag caggcaatcg
     4801 gtgtatttgt cgcaccagaa aatactccga taaagcccaa tcctgcaaga aaaggaacag
     4861 aaacatggag caaaggcgct agtgctgcac caagcgttgc accaatttca aacaatggtg
     4921 tcacttctcc cccttgatat cccgcgccta aagacaaaac tgtgaagaat aacttcccaa
     4981 taaaatcata gacatgtgcc tgtccattaa atgattcttg aagtaacgga agactcaatc
     5041 cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac aactacagct ccacctatcg
     5101 tatttcgaca aacaggatga ggaatccatt gtgcatatcg cgcttttaaa aagacgatcg
     5161 aacggctgaa tacccaacca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga
     5221 acagcaacat actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat
     5281 agctttctgt catgaaattt gcaaacagtg cagcaaagaa gctagggaag atcgcttctg
     5341 ttcggacttt cccgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc
     5401 caaatacaga actaaaaccc gcactgattc cactgataat caagatttcc cgttctgatt
     5461 tatccaatcg gaacaaacgt gccgtattat ctgctaacgc tccccccatt tggactgctg
     5521 ttccttcacg gccaacagat cccccaaata aatgggtagt tatggttcca aacagtgtaa
     5581 gtggaataag ccgcaacggg attttttctt ctccaccatt tccttgatcg atcactaagt
     5641 tgtttccacg actagaaagt ccgccgtgat acgcatagag ataagcaaaa agcgcaccac
     5701 taactggtag tagacaaagg agccaaggat gtgatagtcg gatatctgtc acaactgtca
     5761 ggctatttaa gaaaaaagca gacaagctcc ccatgaatat accgagaatc gctgtgatca
     5821 ccagccactt cagcatatac actaaaatgc gtactggact cggtaattgt ttttccataa
     5881 aaaattcctc ctcaataaaa aaacgcctac taaagaaagg ggctgtcccc agactttagt
     5941 aggcgtcatt agctatcttt cgttagcatt taaaggcgaa caccatcacc tattcacttc
     6001 cattaaaaga tagcatattt ttctgtaaaa aacaataaaa ttatcgttct ttctttacac
     6061 gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg ttgattacat tggtcactat
     6121 catgacaaat ataattcccc tttttcgtat acgtgccatc agaacctgct ttcgttgtag
     6181 aaagaaaaag cgaaacattc gatgttttct gacagattgc acagattccc ttatttatcg
     6241 ttggtgataa agtaccgcta atccctttta atttttcatc ttcgtaatag accatgaatt
     6301 ttttctgcgt acctggatca ttccagccga tgtacgtatg ttcttgcaag tcgatttcat
     6361 tccaagctgg ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc
     6421 taggctgcat aaacggctga acgatttgct tcagctcctc caatgctctt tctgcttgcg
     6481 cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt atcgattctg gcagttcgtg
     6541 atctaatatg tctcgaatct tttcagctgc tagtgcctga actgtttgta ccgtttttgg
     6601 atcattgaca gatgagtacg catttaataa ctgttctact tctcttatta taaaaaaata
     6661 ttggtatggt tttattgttt gttccatgat tttaaagtcc tttcttctaa ccaaagtgca
     6721 tactttgtag aaaaaagtga tcttctgcaa agtatgtgtt acctgaagat cccggactct
     6781 tttaaataag atacgttttg catcttatca tcaccccctt taatgcttat tattctatgt
     6841 acatgattca ttcgataaat aaaagtaaac cgtatcttga acagatagtc aactgcaaac
     6901 agaaaaaagg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaatg aatggtgttc
     6961 aaaagctggc atctgcggta ataagccgaa acttcacaaa aatttgagaa gcaattttcg
     7021 taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaat atggtctatt
     7081 attggtttta tctattaaat cgaggattct ttcccaaagt aacgttgcca tctcttacgc
     7141 atacttgctg acataggctg agcggcttct ttgactgcgc agtatttgtc tacgaatgtt
     7201 acgatatatc cttctttgta cttaggcggt gcaatcgttg cgccccacat gtgcttcaaa
     7261 atgatatctc gctcaagatc cgacaattcg gtgatctttt ctgcattctt tacagcaatt
     7321 cttggatgga tatacgcatg tgtgccttca tcaaatttcg tcgtacgcca atcatagtag
     7381 aacagatcat gcaaaagtcc agcacgcgct gttgctttag cattgccacc ccattttttt
     7441 gcgagcagat aactatagta agaaacactg atggaatgct ccaaacgagt ggaatgaaca
     7501 tgctgtgtgt aattcgctaa ttttttcact gcttctgttt ctaaaagatc ttctacataa
     7561 gacatatatt cttcgtcttc acgccatttt tctgtcataa ttatcgatct gccacctttt
     7621 tgaatttcat tttcttctga aattgatcta agtataccac cagtgttcgg attattctag
     7681 tcgccttgga taaaatagcc aaagcttaat agttccttaa tcataaagaa aaatgataaa
     7741 gaacaatccc tgccgcaacc ccaacattca acgattccgc atttcctttc atcgggatat
     7801 atacgttctg atcagtcaaa gacaagattt cctgacttac cccctgacct tcgttcccca
     7861 tgattattgc gaagttatct gatttttcta ctttgtctaa tgcgtgtgct tcttcattta
     7921 attctgtgcc atacactgta aaccctttgt ctttcattaa aggaatcact tctgtcaaat
     7981 ctttttgcaa cacaggcaaa tgataattgc ttccctgcat gctgcgcagc acctttgtac
     8041 tataaatatc tgccgttcct ttgcctaata aaacaccagc catatcaaat gcatctgccg
     8101 tacggatcat cgtccctaca tttccaggat cttgtacatt atctaacaac agccatcctc
     8161 ctgaaaaatc tggcgtgcct tcagccgtta acgccatcac tgctaagatt ccttgtggtg
     8221 taggtagatc cgatacagca tccatgacct catccgaaac tagaaaagta gaaatctctt
     8281 tgtttgcggc cagccagtct ccccattctt tcatgcctcg ctgtgtcaca aaaagttctt
     8341 ttatttctgc atcagacgac gcagcttctt caatcagatg gaacccttcc aaaagatatt
     8401 cttccttttc ttcccgatat ttctttttat gtagtttttt taattctttg attcttggat
     8461 tttttgctga ttggatctct ttcacattcc tcacctcccc atgattatat catggaatag
     8521 gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt aatttataaa ataggaaaga
     8581 aaggtgacga acatgcgcaa agttaaaatg aacgtccaag gacgcgtcca aggcgtagga
     8641 tttcgctaca tgaccaagat ggtcgctgat caactaggcg tgactggaac ggtaaaaaac
     8701 gaagatgacg gctcagtcac gatcgaagca atcggcaatg acgacatcat ccagaaattt
     8761 attgaagaag tcaagaaatc tccgagccca agtggacgag tgcagtatgt ggatgtccaa
     8821 gaagacccga tgattgagga acgaaagaaa tttgatgtgg tgggataagg ttgtgaaaaa
     8881 gctggtctgc atcaagcatt aaggacaggt tgtgaaaaat agctctccat atttttcaac
     8941 aggctggact taatgccgta gatgcaagct tttgaacacc gtcgataagg ttattcttga
     9001 ggcgctttgc ctcaaaaatt aaggacagca gtgaaaaagc tgatctatca cttgcagtga
     9061 gttcatttga ctccgctttt tcgggcagaa aaaaagtttt tagtttatta aagtaagaaa
     9121 gaaacacttg gaatcattct actttttcct agtatttctt tcttgatttt ttgtcttttt
     9181 ttcaaaattt ttcgctacgt aaaagaacga atatttatat gaaatttcta tgaagttgat
     9241 tgaattccat gcgctttttt agtatagtta agtttgtgta aaaattatag aagaggaagt
     9301 attctatgaa taaatggaaa aactggttat tgggatccgg acttgtcagt atcctgctct
     9361 ttttatctgg ctgtgtgcgg atggattcaa acggaaaccc tgacacttcg ggaatcgttt
     9421 atcgagtact cgtccatcct atgggccaag cgattacata tctagttgaa aatttcaatt
     9481 ggtcttatgg ctgggcagtt attgcaatga ctgtgatcgt ccgtatcatc atcttgccac
     9541 taggtattag tcaatcgaaa aaaacaatga tccaatctga aaaaatgcaa gcgttaaaac
     9601 cacaagttga agcagcccaa caaaaattga aagctgcaac aacacgtgaa gaacaaatgg
     9661 cagcacaagc agaaatgcag caagtatacc gcgaaaacgg attgagtatg accggcggaa
     9721 tcggctgtct gcctctattg atccaaatgc cgatcttctc tgccttgtac tttacggcac
     9781 gttatacaga aggtatccgg gaatcatctt tctacggcat cgatttaggc tcaccaagta
     9841 tggtacttgt tgccattgca ggggtcgctt atctgcttca aggatacatc tcaacgatcg
     9901 gtattcctga agaacagaaa aagacgatgc gtacgatgct gatcgtctct cctgctatga
     9961 tcgtcttcat gtcgatcagt gcgccagccg gtgttacact ttactgggta gtcggtggtg
    10021 tgttcagctg tctgcaaaca ttcatcacaa acgttatgat gaaaccacgt ttgaaagcac
    10081 aagtggcaga agagttgaaa caaaacccac caaaacaagt cgtcactcca agaattgatg
    10141 tgacagaatc agctaaacca gctgacagca aacaagcaac caaacaaatc aagcaaccca
    10201 aaaacagtac aaacaataaa ggccgcaacg caggaaagca gcagaagcgg aagtagatag
    10261 taaataagag tgtaataaaa attcattaac agttttagca attaaaaata tcgaccaaga
    10321 tttttgaaaa agaaatctgg tcgatatttt tatttaagtg aatgtttaat ttgaatcatc
    10381 gattgatcgc tatctaattg cagcagcatc tttttatgaa gaaacaactt tttcttatct
    10441 atcaacgtag acaggtgttg ctgtttcttc taatagtgta cttaattcct taatattttg
    10501 ttttccttct tctaacatcc aactactcgc tttttcttcg ccttcggtaa caaaaggcgc
    10561 cactcccttc gcccatgttg ctcgcccaca taagacacca ttctccataa aggacaacgc
    10621 ggagatacca cccatctaaa taacatccat aaacctttct agtagaagaa tagtgatagt
    10681 atccatccct ttttctactg ggaatacaat tacacgattc aaagcttgat acacaacatt
    10741 tcccttctta taaaccgcta ttgtaaccga attgttttga atcacctttt tgggatagaa
    10801 actggctcct ttccgcaact tcttaaatgt tggtttcttt tatactttct ttgtagcaac
    10861 tacaaatttc ttcaaatctc taaatgtttg cataacaacc gcctttctga cttttactca
    10921 ggatggtatt taaaaatcag caaaataagg cttctctcca tcttttttat ttcctagctg
    10981 ctttaaagac actcttgctg ttttttaatc tctatcaagg gggctctgaa tctattttga
    11041 tgaacttatc ttattaattt ctcaaatgag ctgtcagaca caactgtatt ttttcaatct
    11101 cataccggac aactatgcca gcttaaaaaa atttcctctg gcaaaacttc tttattgaaa
    11161 aatgacaatg aaaaaagacc attctttttg ttgaatgctc attgtgtaaa catatttcct
    11221 gcgactgctc ttcgaaccat tctcagtgca atccctaatt tatctattaa atcttctttt
    11281 agttcctcat ctttcatatt caaaatttcc tttgtatgtt acacaagttt gttctctaaa
    11341 taacgcgaat tgtaaaatta agttagaaaa ataaaaaggc atttatggta cactcaa
//
LOCUS       DACDYM010000078        11254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDYM010000078 DACDYM010000000
VERSION     DACDYM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.079"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWM59_002195"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWM59_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9980212.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWM59_002196"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWM59_002196"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9980213.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IWM59_002197"
     CDS             complement(2169..2906)
                     /locus_tag="IWM59_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9980214.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IWM59_002198"
     CDS             complement(2997..3524)
                     /locus_tag="IWM59_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9980215.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IWM59_002199"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IWM59_002199"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9980216.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IWM59_002200"
     CDS             complement(4401..4886)
                     /locus_tag="IWM59_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9980217.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IWM59_002201"
     CDS             complement(5039..5236)
                     /locus_tag="IWM59_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980218.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWM59_002202"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWM59_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9980219.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWM59_002203"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWM59_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9980220.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IWM59_002204"
     CDS             9211..9423
                     /locus_tag="IWM59_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9980221.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IWM59_002205"
     CDS             9425..11104
                     /locus_tag="IWM59_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9980222.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DACDYM010000079        11002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDYM010000079 DACDYM010000000
VERSION     DACDYM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.080"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IWM59_002206"
     CDS             23..646
                     /locus_tag="IWM59_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980223.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IWM59_002207"
     CDS             1096..2145
                     /locus_tag="IWM59_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9980224.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IWM59_002208"
     CDS             2142..3023
                     /locus_tag="IWM59_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9980225.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IWM59_002209"
     CDS             3026..3796
                     /locus_tag="IWM59_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9980226.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IWM59_002210"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IWM59_002210"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9980227.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IWM59_002211"
     CDS             5107..6270
                     /locus_tag="IWM59_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9980228.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IWM59_002212"
     CDS             6387..6797
                     /locus_tag="IWM59_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9980229.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IWM59_002213"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IWM59_002213"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9980230.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IWM59_002214"
     CDS             9337..9654
                     /locus_tag="IWM59_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980231.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IWM59_002215"
     CDS             9814..10050
                     /locus_tag="IWM59_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9980232.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IWM59_002216"
     CDS             10047..10445
                     /locus_tag="IWM59_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9980233.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3841 a         1820 c         2296 g         3045 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa tt
//
LOCUS       DACDYM010000080        10753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDYM010000080 DACDYM010000000
VERSION     DACDYM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.081"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..1042)
                     /locus_tag="IWM59_002217"
                     /pseudo
     CDS             complement(59..1042)
                     /locus_tag="IWM59_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="IWM59_002218"
     CDS             complement(1437..2279)
                     /locus_tag="IWM59_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9980234.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="IWM59_002219"
     CDS             2604..3020
                     /locus_tag="IWM59_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9980235.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="IWM59_002220"
     CDS             3017..3487
                     /locus_tag="IWM59_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9980236.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="IWM59_002221"
     CDS             3501..4274
                     /locus_tag="IWM59_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9980237.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="IWM59_002222"
     CDS             4288..5100
                     /locus_tag="IWM59_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9980238.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="IWM59_002223"
     CDS             5154..6119
                     /locus_tag="IWM59_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9980239.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="IWM59_002224"
     CDS             6256..8979
                     /locus_tag="IWM59_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9980240.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="IWM59_002225"
     CDS             8992..9624
                     /locus_tag="IWM59_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9980241.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="IWM59_002226"
     CDS             9829..10056
                     /locus_tag="IWM59_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP9980242.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="IWM59_002227"
     CDS             10125..10391
                     /locus_tag="IWM59_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9980243.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="IWM59_002228"
     CDS             10381..10650
                     /locus_tag="IWM59_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9980244.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3566 a         1715 c         2102 g         3370 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctg
//
LOCUS       DACDYM010000081        10500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDYM010000081 DACDYM010000000
VERSION     DACDYM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.082"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(210..962)
                     /gene="xth"
                     /locus_tag="IWM59_002229"
     CDS             complement(210..962)
                     /gene="xth"
                     /locus_tag="IWM59_002229"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9980245.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1075..1995
                     /locus_tag="IWM59_002230"
     CDS             1075..1995
                     /locus_tag="IWM59_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9980246.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2068..3003
                     /gene="murB"
                     /locus_tag="IWM59_002231"
     CDS             2068..3003
                     /gene="murB"
                     /locus_tag="IWM59_002231"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9980247.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3301..4779
                     /locus_tag="IWM59_002232"
     CDS             3301..4779
                     /locus_tag="IWM59_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9980248.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5056..7116
                     /locus_tag="IWM59_002233"
     CDS             5056..7116
                     /locus_tag="IWM59_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980249.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7199..7768
                     /locus_tag="IWM59_002234"
     CDS             7199..7768
                     /locus_tag="IWM59_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9980250.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7943..8749)
                     /locus_tag="IWM59_002235"
     CDS             complement(7943..8749)
                     /locus_tag="IWM59_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980251.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8887..10401
                     /locus_tag="IWM59_002236"
     CDS             8887..10401
                     /locus_tag="IWM59_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9980252.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3724 a         1750 c         2068 g         2958 t
ORIGIN      
        1 ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt cttattactt
       61 gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc agtttctcgg
      121 tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt tcttaatact
      181 cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac tggacaatga
      241 tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc taaatctttg
      301 ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt gaatcggtag
      361 ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt atcgataaac
      421 ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc agcatttttt
      481 tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag atccccacac
      541 acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc ttcttcccat
      601 tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta acaggttaca
      661 agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg gatatcgata
      721 cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt gccggaatag
      781 ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa atcaatttgt
      841 cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc atgaaaaact
      901 tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga gataaatttc
      961 aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt gtacctcatt
     1021 ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa gtagatgaag
     1081 attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac ttatgcgaaa
     1141 aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa agaaagaatt
     1201 caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt agaagaaaaa
     1261 atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc caaaaaatgt
     1321 ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct aaatgaagtt
     1381 cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg tttcaaccgg
     1441 cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt gatccacctg
     1501 caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga tttgttttta
     1561 catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac aaaataccag
     1621 ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac acaaaatcaa
     1681 acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata tcaagtaaca
     1741 agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca agatgctgat
     1801 ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg cgggtttgag
     1861 ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac agaattgctt
     1921 aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa agatcactac
     1981 cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa taatgaggac
     2041 gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga aagcttagcc
     2101 acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa aacaggtgga
     2161 ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat catcgattat
     2221 tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct gatcgttcaa
     2281 gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat tcatgtcaaa
     2341 ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta tgcagctttg
     2401 gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt cggtggagcg
     2461 gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc tgaagtggat
     2521 cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt attttcttat
     2581 cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt cagtttgcaa
     2641 acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt gcgtcaatca
     2701 aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt aggacactat
     2761 acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg tgcacaagtc
     2821 tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac agattatatt
     2881 gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc tttagaaaca
     2941 gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag tttagccaat
     3001 taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc ctgctctttt
     3061 ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata aaacaaaaaa
     3121 tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa aaaagataaa
     3181 aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt ataggtgaaa
     3241 agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga ggtgtaggat
     3301 atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct agctagatat
     3361 ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac attaggtttg
     3421 tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac tgaattcaaa
     3481 acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg tctgaatttg
     3541 acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc tcgactgtta
     3601 acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca ttttttaagc
     3661 atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca gcatcaaata
     3721 aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt attatttggg
     3781 gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt catgaatgaa
     3841 gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc tgcaacaaaa
     3901 cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg gaatcaacca
     3961 atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat gaagatcatc
     4021 aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat tgatgagatt
     4081 tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg cgcatttgga
     4141 cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc agtggaattg
     4201 attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata tatacgaaaa
     4261 gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga ctttgagatg
     4321 actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc aatattcagt
     4381 acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa agatgaacgt
     4441 ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg tccggtatca
     4501 tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc ttataccgat
     4561 tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga gagtcgaatc
     4621 acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa taaagaacag
     4681 ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa taccatcatc
     4741 agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat ggcagctttg
     4801 tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact agatataact
     4861 agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat tattccgatt
     4921 ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct tcttttgatt
     4981 ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga caagagtagg
     5041 gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt
     5101 cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat gatgcgcacg
     5161 atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc aacgacacaa
     5221 accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat cactattact
     5281 catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc ctataataaa
     5341 gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac attatggaca
     5401 tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga tcctacaaaa
     5461 ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga tataaaggac
     5521 ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt tgaaagaaag
     5581 aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga aaggctgaag
     5641 aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg gaatatcgtg
     5701 gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc aattgcttta
     5761 aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc tatcaaagga
     5821 aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt tgaatatgta
     5881 acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg gacagtagat
     5941 actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa aacatcaaat
     6001 gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc gactgtcatc
     6061 actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca tatgagtagt
     6121 gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt gccaaatata
     6181 gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg gatgcaagca
     6241 aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca ctcagaactg
     6301 atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta tataccagac
     6361 aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa
     6421 gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa ggatgtatcg
     6481 gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac aggtgatttt
     6541 acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc tcagtcacta
     6601 ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag acaagaagaa
     6661 agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg gaaagtaaaa
     6721 caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc taataaaacg
     6781 ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga aattttagag
     6841 cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga agatcagaag
     6901 gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga ttccaccgat
     6961 gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc tggtggaggt
     7021 attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg ggtatacgct
     7081 aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc tgttatagtc
     7141 aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg atagaaaaat
     7201 gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc aacaaaatgt
     7261 tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag aagatgaatc
     7321 tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg aagcacttga
     7381 gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga tgttttttgt
     7441 agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga
     7501 gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag gaaaagggct
     7561 cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata cggacggacc
     7621 aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata aaaaaatggg
     7681 ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg aaaaagttat
     7741 gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc agtattggga
     7801 taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact atcggataac
     7861 taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg tcactgcccc
     7921 ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa ttcttgatga
     7981 acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg aaaacaactg
     8041 ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg tctaagtaac
     8101 cattttggat gattgtttta tctgttttac catctgcatt ttgactttca atgattgtcc
     8161 gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc acactattga
     8221 tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct gctgaaaatt
     8281 ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca actgctagta
     8341 ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca agggaaaagc
     8401 cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac attgaaaaat
     8461 agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa gataacaaaa
     8521 tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa taagggattt
     8581 caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg ttccagacct
     8641 gatccactag cataataacg acgatgatac gtaataaaat atagattccg tttgtgattc
     8701 tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata aaataactcc
     8761 ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat atgacttaaa
     8821 gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga gaaaaggggg
     8881 atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct tgctatgagc
     8941 gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa ggaatctgcc
     9001 gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat gaaagaatct
     9061 tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat cgaagcaaaa
     9121 gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg aaacacatct
     9181 aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga aggaatcaaa
     9241 gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac gaatgataaa
     9301 gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct agatcaaaac
     9361 ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag gacacatcgt
     9421 ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa taacgtccat
     9481 gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa aaatggggaa
     9541 acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt caccggaaaa
     9601 gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga aaaatatcgt
     9661 cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga tggtatcacc
     9721 atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat tcatccaact
     9781 gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg agcaatcttg
     9841 gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa agtatctgca
     9901 gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg cgtgcgtgat
     9961 cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg aaagacaatg
    10021 gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac agttgatgga
    10081 tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac agccatggaa
    10141 catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat ccattacacg
    10201 atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa ccagccaatt
    10261 agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa ccggatcggt
    10321 ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca agcagctcga
    10381 tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga aatcagcgtt
    10441 ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt tgtgaaaaag
//
LOCUS       DACDYM010000082         9974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDYM010000082 DACDYM010000000
VERSION     DACDYM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.083"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            37..1068
                     /locus_tag="IWM59_002237"
     CDS             37..1068
                     /locus_tag="IWM59_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9980253.1"
                     /translation="MIAELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQG
                     TIVDFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQ
                     DAWLVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGI
                     VDPFTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQ
                     LVSAHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKAN
                     IKDVNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLF
                     QQGRQQERE"
     gene            1079..3283
                     /gene="topB"
                     /locus_tag="IWM59_002238"
     CDS             1079..3283
                     /gene="topB"
                     /locus_tag="IWM59_002238"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9980254.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            3522..4115
                     /locus_tag="IWM59_002239"
     CDS             3522..4115
                     /locus_tag="IWM59_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9980255.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            4131..5030
                     /locus_tag="IWM59_002240"
     CDS             4131..5030
                     /locus_tag="IWM59_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9980256.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            5033..5518
                     /gene="ssb"
                     /locus_tag="IWM59_002241"
     CDS             5033..5518
                     /gene="ssb"
                     /locus_tag="IWM59_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9980257.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5540..5797
                     /locus_tag="IWM59_002242"
     CDS             5540..5797
                     /locus_tag="IWM59_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980258.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5956..6078)
                     /locus_tag="IWM59_002243"
     CDS             complement(5956..6078)
                     /locus_tag="IWM59_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9980259.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6268..6492
                     /locus_tag="IWM59_002244"
     CDS             6268..6492
                     /locus_tag="IWM59_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980260.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            6940..7146
                     /locus_tag="IWM59_002245"
     CDS             6940..7146
                     /locus_tag="IWM59_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9980261.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7146..7397
                     /locus_tag="IWM59_002246"
     CDS             7146..7397
                     /locus_tag="IWM59_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980262.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            7412..7849
                     /locus_tag="IWM59_002247"
     CDS             7412..7849
                     /locus_tag="IWM59_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980263.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            7842..8549
                     /locus_tag="IWM59_002248"
     CDS             7842..8549
                     /locus_tag="IWM59_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980264.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            8930..9109
                     /locus_tag="IWM59_002249"
                     /pseudo
     CDS             8930..9109
                     /locus_tag="IWM59_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9171..9355
                     /locus_tag="IWM59_002250"
                     /pseudo
     CDS             9171..9355
                     /locus_tag="IWM59_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
     gene            9389..9820
                     /locus_tag="IWM59_002251"
     CDS             9389..9820
                     /locus_tag="IWM59_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP9980265.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         3600 a         1700 c         2077 g         2597 t
ORIGIN      
        1 ccagagtggc tgattgatta tgcagaaaaa gaagggttga ttgctgaact aaagccgaaa
       61 catgaacggc aaaatttttt agttggagac gaccgtttag atcatgcggt tgctttttta
      121 tggaaagatc cacagacagg agagacagta ggggcaagtt atcaaggaac gatcgtcgat
      181 tttaatcgtt tcggtaaacg gggaacgtat aaacacattg acaagaatcc tacgccaaat
      241 cacggattta atttaaaaat aggcgatccg aagcatttaa aattctttga aagtagtatt
      301 gatctgttaa gttacgctgc attgaatcgg gagaaactac aagatgcctg gttagtttcg
      361 atggacgggt taaaacatca tgtaatcagt cattacgttg aagagtcgat ttctgaatta
      421 agacgaaaac aaacatttcc tcaatcgatt gaaatatgtg tggacaatga tcgtgcagga
      481 catatttttt atgaaaaaga acagatgaag ggaatcgttg atccttttac caataagaaa
      541 atccgttgtg agcgaggaat tccaaatgat tggcaagtac ctaaagaata caaagctact
      601 tatgaagcag tcgcaaaaga aatgagtgtt gaacctgaag caattatggc catacacaaa
      661 actgaaacta atttacagct gacaaatcaa ttggtgtctg ctcatgatgt tcaatctacg
      721 tttggaaaga tgttggcgaa gggcgaacca gtagaaacaa ttgatttgaa agaagcatgt
      781 accacggtcg cgaaggaact gaaagtatgt gaacgcgcag atggaacata caactttgat
      841 cgtttttata gtagaaaagc caacataaaa gacgttaatg caggaatcct tctttcctat
      901 aaagcagaac aatactacaa aggctataaa aagcatgaac atgaatttgt acctgaagtg
      961 aaaaaggatt ggaatgatca attgaagcat gagatccagc aacaagaaat cagaaagcaa
     1021 aaacgtgcga tgttgtttca acaaggcaga caacaagaaa gggagtgaag tcgtcactat
     1081 gaaaaccgtc attttagcag aaaaaccgtc acaagcgaag gcttatgccg atagcttttc
     1141 taaagctacc cgaaaagatg ggtattttga gatccaagat cgattgttta caggagaaac
     1201 tgtgatcacg tatggttttg gtcatttagt cgagttagat tcgcccgata tgtacgatga
     1261 aaaatggaaa caatgggcat tagaacattt gcctattttc cccaatcaat accactatca
     1321 tgttcctaaa gataagaaaa agcagtttag tgtcgtcaaa cgacagctgc agtcagctga
     1381 tacgattgtg atcgcgactg atagcgatcg tgaaggggag ctgattgcct ggtcaatcat
     1441 tcaacaagca ggcgccaatc aaggaaaaac ctttaaaaga ctttggatca actccttaga
     1501 aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga cctatccaaa
     1561 atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga acggtagccc
     1621 actgtatagt ttactcttgc agcaaaaagg cataccagga agtttttctt taggtagagt
     1681 ccaaacccca acactctata tgatttatca attgcaggaa aagattagaa acttccaaaa
     1741 agaaccatat tttgaaggaa aagcccaagt aatcgctcaa aatggcgcat ttgatgcgaa
     1801 gcttgatcca aatgaaaccc aagcgacaca agaagcattt gaagcctatt taaaagaaaa
     1861 aggtgttcaa ttaggaaaac agccaggtac gatccttcaa gtcgaaacag agaaaaaaag
     1921 tgcggctagc ccacgtcttt tttctttatc gagccttcag tctaaaatga atcaactcat
     1981 gaaagctagt gccaaagata ctttggaagc catgcaaggg ttgtatgaag gaaaatatct
     2041 aagctatccg cgaacggata ccccttatat tacggaaggg gaatatgcgt atctgttgga
     2101 tcacttagac gaatacaagc attttttaaa agccgaagcg attccgactc ctattcatac
     2161 gccgaatagt cggtatgtga ataacaagaa agtccaggaa cattacgcga tcattcctac
     2221 aaaaacggtt atgacagcag cggcttttga acaactaagt ccactgcagc aggccattta
     2281 tgaacaagtg ttaaaaacaa cggtggccat gtttgcggaa aaatatacct atgaagaaac
     2341 aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag taccgataga
     2401 tctaggttgg aagaagttat ttggcaaaga gaacgacgga aaagagaagg aagaagagcc
     2461 gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag tccttgaaaa
     2521 agaaaccaag ccaccacagc cttataccga aggtacgttg atcacggcga tgaaaacagc
     2581 tgggaaaacc gttgatagtg aagaagcgca atccatattg aaagaagtgg aaggaatcgg
     2641 tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat atatcaaggt
     2701 agagaaaaat aagctcgtgg tgacgaataa agggattttg ctttgccaag ccgtggaaaa
     2761 agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact tgttaaagat
     2821 cggtgaacga aaaggaacac aaaccacttt tctagccaat atccaaaaat ttgtctctca
     2881 cttattggaa gtcgtaccag ggcaaatcca atccactgat tttggatcga cgttacaaga
     2941 agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt gtccaaaatg
     3001 ccaggaacaa gaagtcttac tctatcaaaa ggtggcagca tgtacgtctg aagcctgtga
     3061 tttcaaacta tggacaacga ttgccaaaaa gaaacttaca gcgacacagc tgaaagagat
     3121 cattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga agggttcgtt
     3181 tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt ctgaaaagaa
     3241 aaaaacgaac ttcaagaaaa gaacacgacg cactacaaaa taatggaggg ttaatcttat
     3301 gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga
     3361 acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa ttgaagaacc agcagcggat
     3421 tcaaaaagaa atcgaattag atcgataaat tcctttcttg ttaatataaa actaacatgt
     3481 tatagttgtg ttagttttat attaacttta ggggaagagt aatgaaagac aaaatccaat
     3541 gtatttttga taaacataat ggcaacctat ccttgaagga tgctagaagt gaaggattat
     3601 cgcctatgac gttagatcga ttagccgaaa aggataaaat agaaaaggta gcaccaggtt
     3661 tctatacctt aaaggatgcg ttcgtggatg aattgtatct cgctcagagt atctattcca
     3721 gagggatttt ttctcatgag actgcgctcg atctttttca agtaggaaca tatatcccta
     3781 aaaagatcaa tatgatgttt cctaaaggat acaatgtatc aaaggagaaa ttagatcagt
     3841 atgccattca accgcattac actaataaag aaaattttga cctgggagtc acagaaacag
     3901 aaagctttta cggcaataaa attttagtct atgatttaga gcgaacgtta tgtgacatgt
     3961 ggaatccaag atacaaagca agcgtagagg taaaacaaga agcattaaaa gaatatatga
     4021 caagtaccag tagaaataca aataagctaa gaagatacat ggatatttta aaatcaccaa
     4081 aagaaatgac tttgtatatg ttgccacttt attgagagga gtaaagatga atgacaccga
     4141 cacaatttaa agagaaaaca agaaatttcg cagcagagaa acatttaaac ccacaaattg
     4201 tccaaagata ttatatgatg gaaaaattat tagaatgtat tgttgaatcg ccttatcatg
     4261 atgattttgt attaaaaggg ggatttctga ttggatcaaa atacgggtta gaaaaccgtt
     4321 caaccgttga tattgatacg acttatcgaa attcgaaact aacaaaagaa aagttagaaa
     4381 ctgttttttc tgaattaaca gctaatccta caaaagatgg aattcgtttt gaattaaagg
     4441 gattaacaga gacgagggaa gccgattatt atccaggttt tcaagcaaag tttattgctt
     4501 atcttgaaaa tgcacgtgta ccttttaaat tagatattac ttcaggtgat tctattattc
     4561 cagcagcatt gatgtatgaa cacaaattaa tgtttgaaga taaaacgatt catattccag
     4621 cttatcctac ggaacaaatt ctagctgaaa aattacatgc cacgtttagt tttggaaaag
     4681 ataattcgcg catgaaagat ttttatgacc tgtatatgat tccgaaatta gaacgtatcg
     4741 atgagaaaaa tttgtataag agtgttcaag gaacattcaa ggaaagaaat aatcaatcgg
     4801 tgtttaagca tttgtatgat caggaaatgc ctcaattggt gaaagatgag ggcttgagag
     4861 aaaagtggaa aagataccaa caagaaaact attttgccaa agacatttct ttcgaatcta
     4921 cactaacttc cgttgattcc ctgatgcaat caattgtaga gcaagagaag gcagaacgaa
     4981 aacaaaattt taagagatca caacaaatga atcaagaaat ggagagataa agatgatcaa
     5041 taatgtaact ttagtagggc gattaactaa agatccagat ttacgttata ccgcaagtgg
     5101 cacagcagtg gcgacttttt ccctagccgt taatcgtaat ttcacgaatc aaaacggcaa
     5161 tcgagaagct gattttatca attgcgtgat ttggcgcaaa ccagcagaaa cgatggcgac
     5221 attagctaaa aaaggcagct tgatcggcgt tgtcggacga attcaaacac ggacttatga
     5281 caaccagcaa ggacaacgtg tgtatgtgac agaagttgtg gccgataact tccaactatt
     5341 agaaagtaaa gcagcgacag aaagccgtgc gcacgctgat caatcgtcca ccagtccaag
     5401 caccgccacc tttgaacaac ggaatacagc gacgcctaac aacaatggat tgaacgcttc
     5461 acagaatcca tttggtggac aatcaatcga tattagcgat gatgatctac cgttttagtt
     5521 tgagaaagaa ggagtaagca tggcaaaaaa aattagtctg acgatggatc tattgatacc
     5581 cgtaattgca tttatagacg ggatttcttt gggcgtcact gaaaatcctt tggtaaatag
     5641 aattgcgaaa ggatcactat ttcttgtatt cgtgtatctt gtctgttctt atcttttaaa
     5701 aagagcagga aaggataatt ccttttggcg attgactaaa gtcacagtag cagcgacagc
     5761 tgttatactt acggctattc taccgcttta tatgtagagt taacgggaaa tcgttgaact
     5821 tcttgcgaaa tattgctata atgaacatga aaaagaggag ttgcgctaac aacccctcat
     5881 taacagcacc gattaaagaa cggtggcaga atattagtta tttggaaata accgctgacc
     5941 tgctaaagta gacggttatt tctttttgtc atttttaagc atatttacaa tcaaactaat
     6001 caaagcaatg gtaaacatac caaaaccaag aatggtttga attgtctcat atgcggacaa
     6061 aaggcttact cctttcatag attttagcac ttacattcat aagcaccacc acctttccag
     6121 aagtcgccac cgtcttcaaa ctttactgtc ttgactatta tagcatatct aaaatcgaat
     6181 aaaaattctc aattttagct gtttttgcta taattttagc gaggtgatcg tgatggaaca
     6241 atatattggt ttactcgata aattagggtg atcaagacac agccattgct tgtgcgattt
     6301 acattacgca cgagtcatga accaatcaat tgtgtagtgg ccaatataga aatagcggat
     6361 aaattgctga ttttggaaga aggcaagtat actatttcag tgacaggtca cttcaataaa
     6421 cgaaatcaac tagtcattga atcaatgcaa attagaaacc ctgatcattt tacgagatta
     6481 atgggcatat aaataaaaga agagctgcag tcctaaatga ggattgcagc tctttttgtt
     6541 ttacatagaa acaagaaaaa tgaaaaggcc tttttaggaa ggcgcaagcg ccttctcaat
     6601 tgaaaaacct ctctctcact ccaaccgcca ccgccgttcg agaagaaagg aagtgaacgt
     6661 tgcacgttca ctaagataag gggtcttgca aagcaagaca aaatagaaaa aacttcctct
     6721 caccaaaagg ttcgttcctg ttcgctatct ctttattgat ttgccagacc ggcaacaagt
     6781 tgccttttat caccaaaaag cgacaaggac aagggtaaat ggcacgctgc acgcccttgt
     6841 ccttgtcgct tttagttgat tcattctggc caacaaatca ataaagaaag aactaacgaa
     6901 aggaagtaaa acctttccta agaggaggca gaatcaatca tgcgaaagaa atttgattat
     6961 tggggtgttc ccttcagcga attattccca aactatcatg caccatatac tgtcgaatgc
     7021 gattgtggag aaagagcgaa atgtatcaaa agctattgtt tgtatcaatg tccaacatgc
     7081 gggaaaaaat ataccttgtc ttatggcgat tatattttga ttgatgagaa aggaaagaaa
     7141 cgctgatgaa aacacaacac aaattattga ctgtaaaaga agtcgttcaa attttggtgg
     7201 acaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg tactggacga
     7261 tagaaacccc tttagtagag gatgcaccga ctatgctatt tgaagaccta gtgaagattg
     7321 caggatatga actgcattat caagaaaaca tggaaactta tgaaatcgaa tattggttaa
     7381 ccaaattaga gaactaaaaa ggagaaaaaa tatggctcaa aattactatg acgaatttgt
     7441 caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc tataccatga
     7501 aacacgtgta cccaaaaaac attacaagga aaaattgtca gtcgcagtgg aagaaatgat
     7561 tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacactgg aagaactgaa
     7621 aaaacaaaat ggaaaatggt ttttccaagc cttactatgc ttggaagtag gtgtcaaacc
     7681 aagcactatc aaaccttccg aatatcaagc cttagaactg acttatgcaa aattcgcaca
     7741 gattaagaaa gctaaagcaa tggctagcga ctggctagat acttttgaaa gtatcgaaaa
     7801 aaatggtgca ttttatacga ttgaatcgga ggagaaagat aatgaataag gtggtttatc
     7861 agctaacaac agaagatgtt tcgcaatttt taacaagcaa agaacacttg aatgaagtaa
     7921 tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag
     7981 tggaagaaca gttgtatatg gaaagcgaaa caagtcaaac gaaggtcaca gtcagccata
     8041 atcatttaga cgtgtataaa cgtcgtcctg aaacggtaaa aaaaggaaca gagcttctac
     8101 gactagaaac agaaaaatac atcatgaatg tgtacctaga aaggagtgag ctggtcgaaa
     8161 actttgtcag ctacacgatt tgtaggaaac actattactc agaatcagaa tattattcaa
     8221 ttttcgagag catggaatta atggaagaag aaatggaaga gctaaacagc aaaaatgcga
     8281 cggtagacga ttattacctt gtatttacta ggtttttgac gtgtgggacg gaatctatcg
     8341 tgttaatggg aatggattat gaactcgctg tacccgaagc atttttgagg gaatatgtag
     8401 aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt ttagacacct
     8461 acacttacga cgatacagaa gaaatccaaa atgaacttga ccgtaaacga atgccatatc
     8521 aacgatttga aaggaaaaaa ggtcaataac catttatacc gaaaagtgag gatgtgtgaa
     8581 ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgctccaa
     8641 ccgcccccgc cgcttaggtg gtgcgaaata agcgggcaaa gcccgataac ctaagagaaa
     8701 gaaggagatc catatgaata aaatcattcc tctattgttc gtactgtttg ttggcgagat
     8761 acttatgcgt catgctgcag ctgtacattt aaacttcgta gtattcttag gaatactggt
     8821 attttgtttg atcttttggc gatttgtatt agcccttgtg agtctactat tcaagatgat
     8881 tgcagccata tttttcatag ggttatttcg atgaaaggag agagtactaa tgccaattgc
     8941 aataggaaat aaacggttac ccgtgacgtt ggatgaaaag agacaaaaag aattgcagta
     9001 accaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
     9061 gattgcgcaa gaaaaagcag gatttgacat accagcactc aaaaagtgat cgcactttgc
     9121 gcacgtgtta atacgtgtta taattatttt gtaaggaggt atagtgcaca atgccactta
     9181 cgggaaaaga aatgcttaag ttattgaaaa aaatggctgg gttgagcgaa ggcaagaggg
     9241 atttcatcat catctttata aggatggtgt tagaatcact gttcctgttc atgccaatca
     9301 agatcttggc cgaggacttg agcgaaaaat tttaaaggat gcgggattga aataatctcg
     9361 cattcgatta aagaaaggat tgactgaaat gttaagtgag cctttagcaa aaacctatcc
     9421 tgctattttt agcccagaag aaggtggtgg gtactttatt gaatttcctg atgttcaagg
     9481 tgcttacaca ggaattaacg aagacgatat atcttatgga attgctatgg cacaagaggt
     9541 gttaggtatg gttttagcag attatattga gcatgaagat ctacttccag aaccaacacc
     9601 aatcaataag atttctgttg aagatgattc ttttactact ttaattcgtg tagatgttgc
     9661 caaatatttg aaagacacag aacttgtcaa aaaaacctta acaattcctc aatgggcgga
     9721 taagctaggc aaaagggctg gcataaattt ttcagtcctt ctaacagaat caattgcaga
     9781 taaagcagat aacattcttc gttctggaag aaataattag aactcaaaag ggtcagaaac
     9841 ttaaaaatct ttggcttctt tttttactac aaagatggct tctttaaatt atttgcctga
     9901 ttgatttaaa aaaattgcgc aaagactacg agctaattaa gttgctcgtg gtcttttttt
     9961 atgaaaactt ttta
//
LOCUS       DACDYM010000083         9964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDYM010000083 DACDYM010000000
VERSION     DACDYM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.084"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IWM59_002252"
     tRNA            8..81
                     /locus_tag="IWM59_002252"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IWM59_002253"
     tRNA            101..173
                     /locus_tag="IWM59_002253"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IWM59_002254"
     tRNA            201..274
                     /locus_tag="IWM59_002254"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IWM59_002255"
     tRNA            288..361
                     /locus_tag="IWM59_002255"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IWM59_002256"
     tRNA            385..474
                     /locus_tag="IWM59_002256"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IWM59_002257"
     tRNA            487..560
                     /locus_tag="IWM59_002257"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IWM59_002258"
     tRNA            579..654
                     /locus_tag="IWM59_002258"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IWM59_002259"
     tRNA            656..726
                     /locus_tag="IWM59_002259"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IWM59_002260"
     tRNA            740..813
                     /locus_tag="IWM59_002260"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IWM59_002261"
     tRNA            838..925
                     /locus_tag="IWM59_002261"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IWM59_002262"
     tRNA            947..1018
                     /locus_tag="IWM59_002262"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IWM59_002263"
     tRNA            1041..1130
                     /locus_tag="IWM59_002263"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IWM59_002264"
     tRNA            1143..1216
                     /locus_tag="IWM59_002264"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IWM59_002265"
     tRNA            1235..1307
                     /locus_tag="IWM59_002265"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IWM59_002266"
     tRNA            1311..1383
                     /locus_tag="IWM59_002266"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IWM59_002267"
     tRNA            1388..1458
                     /locus_tag="IWM59_002267"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IWM59_002268"
     tRNA            1471..1544
                     /locus_tag="IWM59_002268"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IWM59_002269"
     tRNA            1556..1627
                     /locus_tag="IWM59_002269"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IWM59_002270"
     CDS             1893..3086
                     /locus_tag="IWM59_002270"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9980266.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IWM59_002271"
     CDS             3216..4688
                     /locus_tag="IWM59_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9980267.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IWM59_002272"
     CDS             4771..5559
                     /locus_tag="IWM59_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9980268.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IWM59_002273"
     CDS             complement(5626..6294)
                     /locus_tag="IWM59_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9980269.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IWM59_002274"
     CDS             6409..8121
                     /locus_tag="IWM59_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9980270.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="IWM59_002275"
     CDS             8124..9896
                     /locus_tag="IWM59_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9980271.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2900 a         1781 c         2278 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DACDYM010000084         9828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDYM010000084 DACDYM010000000
VERSION     DACDYM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.085"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..360
                     /locus_tag="IWM59_002276"
     CDS             4..360
                     /locus_tag="IWM59_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980272.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            376..726
                     /locus_tag="IWM59_002277"
     CDS             376..726
                     /locus_tag="IWM59_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP9980273.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            755..1534
                     /locus_tag="IWM59_002278"
     CDS             755..1534
                     /locus_tag="IWM59_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP9980274.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1545..2222
                     /locus_tag="IWM59_002279"
     CDS             1545..2222
                     /locus_tag="IWM59_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP9980275.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2241..3347
                     /locus_tag="IWM59_002280"
     CDS             2241..3347
                     /locus_tag="IWM59_002280"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP9980276.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3337..4428
                     /locus_tag="IWM59_002281"
     CDS             3337..4428
                     /locus_tag="IWM59_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP9980277.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4441..5181
                     /locus_tag="IWM59_002282"
     CDS             4441..5181
                     /locus_tag="IWM59_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP9980278.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5469..7409
                     /locus_tag="IWM59_002283"
     CDS             5469..7409
                     /locus_tag="IWM59_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9980279.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7496..9418
                     /locus_tag="IWM59_002284"
     CDS             7496..9418
                     /locus_tag="IWM59_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9980280.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9698..>9828)
                     /locus_tag="IWM59_002285"
     CDS             complement(9698..>9828)
                     /locus_tag="IWM59_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9980281.1"
                     /translation="HWDLKDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3211 a         1629 c         2181 g         2807 t
ORIGIN      
        1 atcatgatta aagtagttat tgcagaccgt atgggtaaag gacaaaatgt ggccaaaggg
       61 gtagaagcag ctggtggaaa agcagttgtc gtaccaggaa tgggggcaga tatgcgtttg
      121 ggcgatgtga tgcaacaaga gcatgcagat atgggtatct ctttttgtgg tagtggtggt
      181 gcaggtgcat taacagcagc aacaaaatat ggctatccag aacgacatgg tatgcgttcg
      241 attgaagaag ggatcacagc tatcaaggat ggcaaaactg ttctaggttt cggctttatg
      301 gatcaagagg aattagggaa acgtctgaca gaagaattta tcaaaaagca tggaaaatag
      361 aaagagggac atgaaatgag cgttcaaaag acaattcagc aaaaagttat cgtctccggt
      421 aaaggggaaa caaagcaaca tgcttttgca tcagcgttag cagaaattca aaacaaaata
      481 atggaacaat caaacgtatt attgcgaatc gaaccagtca atgtggatat tttaaaggcg
      541 gtcgaaaaca aatacacaga acgctttcta tttttcttct tcccacggca acgtattagc
      601 tatgaagtcg aactctctgt tactgtggct gtaacagaaa taaaattggc agacatatcg
      661 ttcagccagc aaatgactga agatccaaat gggattgcca ttccatttgt aactaaaaaa
      721 atctaatttt gaatcataag ggggaaagaa taacgtggat ttcgtaatca ttctagtcaa
      781 atcggtcatt atcgggggat tacttggttt tgcagcatct gtcggggcag ctcgaatgtt
      841 tcatgcacct aatacacaag gtttaggagc ttttagaaca ttaggcgaaa tgaatgcctg
      901 catgggtgat ccagcttctc atttttcatt tggtttaggc tttttcttta atgcatgggc
      961 ttcttcagtt ggtgctggtg cattcacaca agatgtgacg catcgaatca ttcccaattg
     1021 ggcagcggca gcacttctag caaaaaacaa agatgtcacc cagaccatgc atgatccgaa
     1081 aaaaatgggg atcattggag cggttatcgg ggcagtggtg gtagccttct taaacacaac
     1141 agctgcagca attccagagg cgttacaagt gacggcggtt tccgttttgg taccagcagc
     1201 gacgattctg atcaatacag taatgccagt tattttttgg ttggcagctt tagacgcagg
     1261 gaaacgaact ggtttttggg gcactctttt tggtggtatc gcgcaattga ttatgggaaa
     1321 tgcggtacct ggtgttgttc taggaatctt gattggtaaa ggcgtcgatg agagcggttg
     1381 gaatcgcgtc acgaagagca tgatgatcgc tattgtttta ttatttattt tcagtggatt
     1441 tttccgtgga tttgatatgg aaatgatcaa gagcttccag cttcaagtgc ctaattggct
     1501 tgataacttg cacaacatct ttagtgtgaa ataggaggag aatcatggag acaacagaag
     1561 aacaagcaaa agtattagaa gcaaagcggc aaaagagctt ttggtttgca gactggtctt
     1621 ttccgctaat cgtaggaatt atgtccgctg ctgtatttgc aggaacacac atgtatgtga
     1681 tgtatggtgt gggtgcattc aatgaagttt cgatcgtggc gatgttaaaa gccgggctgg
     1741 atggcggatc ctatggcgca gcggcagcgt ttggtgctag tttcttattt gccagaattt
     1801 tagaaggctc tttagttggg atcttggact taggcggttc gatcctaact gggatcggaa
     1861 ttggggtacc agcaattctc ttaagtatga aaatagcggc accaatcgat aattttgcac
     1921 tctctttatt gactggtttg gttctaggtt tgattgttgg tggcatcatt gtcttgattc
     1981 gtaaatttac gatcaatcaa tcaaattcca cttttggtgc tgacgtcatg atgggggcag
     2041 gaaattcatc tggacgtttt ttgggtccat taattatatt aagcgcgtgt gcagcatcta
     2101 ttccaatcgg cattggcgct acattaggag cactaggatt ctatgcttgg aaaaaaccaa
     2161 ttgctggagg tgctatttta ggagcaatga ttttaggagc aatcttcccg gcagtaaatt
     2221 aatagaaaga gggtcattaa atgtttgata ctttgattaa aaatggaagg ttagttgatg
     2281 gatctaaaat agaggtcgct atagaaaaag gcatgattaa agcagttgca gcagaaataa
     2341 atcagccggc gcaagaagta gttgacttgg agggaaagta ttatttatcc gctgggtgga
     2401 tcgatgatca tgtccactgc tatgaaaaga tgaatcttta ttatgactat cctgatcaga
     2461 taggggtcga aaaaggtgtg acgacggtca ttgatgctgg aactacggga gcagaaaaca
     2521 tccgtgaatt ttatcaattg acgaaatcgg tcaagacaaa tgtgtatgcg ctaatgaata
     2581 tctctaaatg gggcattgtc aaacaagatg aacttgctga tctaacaaag attcaagaag
     2641 atctagtaag tactgcgtta gcagaactgc cggaatttat tgtcgggatt aaagcgagaa
     2701 tgagtaagac agttgttggt gaaaatggca ttaagccatt agaattagct aaaaaaatcc
     2761 agacagaaaa caatgatctg ccattgatgg ttcatatcgg ttctgcacct cctgagttag
     2821 aagaagtatt gtcaagaatg gaaagaggcg atgtattgac ccattgtttt aatggaaaac
     2881 caaacggtat tttagatcca aagacagatc aaattaaaaa ctttgtctgg gatgcttaca
     2941 ataatggtgt cgtctttgat attggacatg ggacggatag ctttaatttc catgtggcag
     3001 aaaccgcact aaaagcagga atgaaagcta cctctattag tacggatatc tacatccgca
     3061 atcgtacaaa tggcccagtt tttgatctag caactacttt agaaaaattg agggtagtcg
     3121 gctattcttg ggaagaaatc attgaaaaag ttacagcagt tcctgcacgg aattttcatt
     3181 tgaagaataa gggccagtta gctgtcaatt atgatgcaga tttgacgata tttgagattg
     3241 ttgaaaatga aaaggaattg aaagattcca acggatttac acgtatagct aaagaacaaa
     3301 ttttaccgat taaaacaatt attggaggcg tcatatatga caattagcta tggaaagttt
     3361 gatttaaaag aagtcattaa tgcatcggga aaaatgacga tcctaggggt ttctaaggta
     3421 tctgaaagtg tcctagcagc acaacgattt ggaggcgagc atttttttga gatgagtgat
     3481 cttgccatca aaaccggcgc ttatctttct caattactga aagtggaaga tgcgcaaatc
     3541 gtttcttctg cttcagcagg gattgctcaa tctgttgctg ctttaatcgg acagggcgac
     3601 atgtaccatg tctatcatcc ctatacagaa aaaatcgcta gacgggaaat catcatacct
     3661 aagggacata atgtagatta tgggacaccg gttgaagtaa tggttgctca aggtggagga
     3721 aaagtcatag aagctggcta tgcaaatatg tgtacaccgg aacatgtttc catgatgatt
     3781 acggaaaaaa ctgctgcgct tctatatatt aaaagtcatc atgcagttca gaaaagtatg
     3841 ctgacggtgg cagaaatggt agaagttgcc aaagctcatc gtttgccatt gatcgttgat
     3901 gcagctgcgg aagaagattt attcaaatat agcgaaatgg gcgttgactt agtgatttac
     3961 tctggggcaa aagcaatcga aggtccgagt gccggactgg tcataggaaa aaagaagtat
     4021 attcaatgga ttcggctaca gtctaaaggt attggccgag caatgaaaat cggtaaagat
     4081 aatattttag gctttaccca ggcactagaa gattatttaa aaaatgggag cgaatccggt
     4141 gactccatga agcaacgatt agcgcctttt gttaacgcaa tcaatcaact tcctgacctt
     4201 tcagcgaaaa tagttcaaga tggtgccggc agagatattt atcgagcaag tgtaacgatt
     4261 aatggcgata aaagtgcgaa agaagtcatt caggaattaa aagcacgaaa tccagcagtc
     4321 tacacccgag aatatcaagc aaataacgga atcattgagt tcgacattcg ttccgtcagc
     4381 caaaacgaaa tggaaaaaat tataaaaaga ttgagagaaa tcatgtagga ggcttttaaa
     4441 ttgtcagtta tcccaaatta ttataaaaat cgtgtatgtt taaatgtatt agctcattca
     4501 gttgaaaatg caaaagcatg ctatgaagca gcggaagggc atgtcgtact aggtgtgcta
     4561 tcgaaaaatt atgctagcga tgaagcagca atcgaagata tgaaaaggta ccaagacgca
     4621 accaataatg cgttgtcagt tggattaggc gctggagatc caaaccaaag tcagatggtt
     4681 tcgcgtttat ctggggtttt acaaccacaa catgtcaatc aagtatttac tggcgtgggg
     4741 acttctcgtg ctttattggg ccaaaatgat acagtcatca atggattggt ttcaccaaca
     4801 ggtaagatcg gctatgtcaa tattgctaca ggtccgttaa gttcacaagc gccagcagct
     4861 gaagtgccta ttgaaactgc cattcaaatg ctgcaagaca tgggaggtac ttcaatcaag
     4921 tatttcccga tgaaaggctt agcacatagg gaagaattta aagcagttgc ggaagcttgc
     4981 gcaaaatatg aattctattt agagccaaca ggaggaattg atctttcaaa ctttgaagaa
     5041 attctgaaaa ttgcaatcga tgcaggtgta aaaaaagtga ttcctcatgt ttatagttca
     5101 attatagata aagaaacagg agatacgcgt ataggagacg taaagatact attaagcatg
     5161 gtaaaaaaaa ttattcacta actcattaga aaataagaag cttattccag aatgaaatta
     5221 aatggaataa gctttttata attaattacg gaaattgtta atgtagataa taatttagat
     5281 gtacgtaagc agaagtacag aaatgtaaga ttttccaata ttattttatt caacataata
     5341 aatcgtatat caattttttt agcgaaattt ctatttattc caagaatttc agttattacg
     5401 gaatcatttt gtgcttaata aaaagaaacc gtttttatat actataaatg tagaaaggcg
     5461 gtaggaaaat gaaaaaaatt attgcaatca catcctgtcc agttggtatt gcccatacat
     5521 acatggcagc agaaaactta gagaaagcgg ggaaagcagt tggcgccgag gtaaaagtcg
     5581 aaacacatgg ttccatcggt attgaaaatg aattgactgc aagagatatc gaagaagcag
     5641 ctggtgtaat catcgcagca gatactaaaa tagataaatc acgattcggc ggtaaacctt
     5701 tgataaccgt aggtgtgcaa gaaggaatcc accatgcaga tgagttggtg agagatattt
     5761 tagcaggaaa agcgccagtc tataaaagta cggaggcaat tatttcttcc gaaaataaaa
     5821 gtgaatcaga aaatttaggt aaaaaaatct ataagagttt gatgaatggc gtttcgtaca
     5881 tggtaccttt cgtagttaca ggagggttat tgatcgcgat ttctttaaca ttaggaggaa
     5941 cagctacacc tgaagggatc aagataccag aaggaacgat ttgggcaacg atgaatagta
     6001 tcggaagtat cgctatgggg ttgatggttc caattctatc tgcatttatt gcgcaaagta
     6061 ttgcagatcg tccaggactt gttcctggat ttgttggtgg gatgttagcg gcaaacggag
     6121 cattatatgg tagtgatgcg aatgcgggat tcttaggcgg gatcatcact ggatttttag
     6181 caggattcat tgctttagga atcaagaaag tgcgagtacc gaaagcttta caatcgatca
     6241 tgccaattat tgttatccca attttaggat cattggcagt tggttttgtg tttatctatg
     6301 ttattggaga accagtagct cttctattta gttcattgac tcacttttta gcagggatgc
     6361 aaggaggaag tgaaattgtt ctagctatga tcttaggagc gatgattgct tttgatatgg
     6421 gggggccgtt caataaagta gcctttttgt tcggatctgg cttgattgca gaaggcaatt
     6481 attccattat ggggccaatt gctgtagcta tctgtatccc tccattagca ttggggctgg
     6541 catcattgat tttaaaaacc aaatttacga aaacagaacg agaagctggt aaggcgtcct
     6601 tagcaatggg actgtttggg attacagaag gcgctattcc gtttgcttca acagatccac
     6661 tacgggtgat tccaagcatt gtaatcgggg caatgacagg aagtgtgatc gcgatgcttt
     6721 caggagtgac agatcatgta gctcatggcg gtccgatcgt agcggtttta ggagcagtgg
     6781 atcacgtagc catgtttttt gtagcagtga ttgctggtat tgctgtaacc gtgctaatgt
     6841 taagaatcct taaaccgaca ttacaacaaa ctgcggttgc tgcagcaaat ggtgtgacag
     6901 tagcagatct atcagagact gaaacaagtg atgtttatac gaaaactgaa acttttgatt
     6961 ctatcacgga tattatgtca gatgaagcaa tcgttttagg tgaaacagca acaacaaaag
     7021 acggagtaat cgaagattta gtccaacgtt tagaaaaaac agcggcgatc acagataaaa
     7081 ccagatttaa agaagtcatc tatgatagag aacatgaaag tacaacggga atcggtatgg
     7141 ggattgctat tccacatgga aaatcagctt atgttcttaa accaacagtg gcatttgcca
     7201 gaagtcaatc tggggtagat tggcattctt tagatggaaa acctgcacat atgatcttta
     7261 tgattgctgt acctgaaaac agccaaggtg atatgcactt gaaaatactg caacgtctat
     7321 cacgtaaatt gatggatgat gacttccgtc aagcactgat ggaagcgcca gataaaacaa
     7381 cagttcacca attattaagc gaaatgtaac ataagatgaa aaatcgaccg tgatgagaca
     7441 taatagatgt gttatcatga tcgatttttt atttagaagg gagtttctgc acattatgaa
     7501 tcagcgtcag caatctttga tctatcaatt aagtgagaac aataattata taacaacaaa
     7561 aaagctagca gagcatttcc atgtgtcaga aagaacgatt catagcgatt tgaataccat
     7621 tgaaacttgg ttgagagaca aacatctgcc tttaatgttt gaaagaaaaa aaggtacagg
     7681 tatcttgttg aatggtctgc ctcacgaaag ggaacaactg aaacgagcgc ttaatgaaga
     7741 gactcaaaca gaaatggata aaaaacagct tcgtcagtta ttgattttcc atctgctagt
     7801 agcaaaagac ggatttagtc ttgaagaact ttcagaaaaa ctatatgttg gaaaacgtac
     7861 gattcgtaaa gaattgacag agttgcaaag cttttttgag tatcaccatt tacagctagt
     7921 atccaaaaca aaactaggaa cttttcttaa aggcgatgaa caagaaaaaa gacagttgct
     7981 tgttaagaca ttacgaaata tgcagaaaga agatccacaa agtccgtcgt tgaaagaatt
     8041 cttcgctaaa gatacattaa aagttatcca acatacatta caagatgttt tttatgaaaa
     8101 ccatttagaa cagccgaatg gactggctag tgtggagatt cacatctact ttatgctcga
     8161 gcgaatgaaa cagtaccaga aagtgaaatt aagtaaagac gaaaacgaag tcgtggaaca
     8221 cacccaagca caaaagttaa gtagtcaaat attagcaaaa ctagccacca tctatccaat
     8281 tgaattttct cctgatgaaa tcaattatct ggctttgcgt atcgccaatc tttttacaaa
     8341 ttcacaagca gccacacgtt ttcaaaagga atctagcgca cttgctgatc atttgatcga
     8401 acaagtggaa cagtttctag gctattcatt aaaagaagat cagctactaa aacaaaattt
     8461 aaggtctcat ttatcttcaa cttattttcg actccattat ggtttgcaga tttctaatcc
     8521 attgacgaaa aatgtgttta gtacgtacac acaacttttc ttagttttac agttaatttt
     8581 agaagattat tttgaaaagg aaaattttta tgttccacaa gaggagacag cttatttaac
     8641 gatccatttc caagcagcga tcgagcgaca caaacatcag aaaagtagaa gatatcaaac
     8701 ggttttagtt agtgaatatt caaaagctat ggctactttt ctcgaagcac ggctgaacag
     8761 agaacttcca gaattaacga ttatggatct agttgaatat aagccagaat taaagagaca
     8821 agattttccc tctgttgatt ttatcttggc tacgttacca tttaaacata taagcattcc
     8881 agtaatcgag atctcaccaa tgatcacaga aactgatcta gcctatttga caaaatatat
     8941 gctggagcat gtgcctatca agaagaaaaa aacatttgat ctagcaagct ttactcatcc
     9001 atttctgatc tttcctcaac ttgaacggac agacccagcc gatattttaa actttatggg
     9061 aaacgtctta gtggaacatc attatgtgga gccggaattc atcgattctg tcttggaaag
     9121 agacagtcac tccagtacta gagtagcacc atttgtaaca atccctcacg gtaatccgct
     9181 gtatgtgaag cattcgatga tttctattgc tacaatgaaa gaaccagtcc tatggcacgg
     9241 ggaacagata cgtatcgtac tgctattagc aataaagaaa aatgatttga aacatgcaga
     9301 gttcaaaaaa atattttctg ttattcacta tctagaaaag cagccagaac aaatggctca
     9361 gttgatgcaa tccaatcatc cgctagaaat cttaacgttg ttatctagtt atgaataagt
     9421 agcgattaaa aaactgcctt tatatttata ataattctat ctatttattt ggaagtagaa
     9481 aactaaatac aaaaaaagag gatgtggcca aagttgtggc acatcctttt attttgagtt
     9541 tcttataatt ttttatagtt gcttacgatg tcttgactac tggtaaacgg tgttcaagag
     9601 acaatttgtc ggcaataagg cacaattttg taaaatatgg aaagctattt ttttaacatt
     9661 gtttcttagt gcttgaaaaa tcattttgaa tacttcttta tacgatcgat gtacgttgat
     9721 ttaagtctaa aacttttctt ttgattcctt ctctgacttt tctgaattcc gttaatattt
     9781 cttcttcgct cccgacagct tgtgcaggat cttttaggtc ccagtgaa
//
LOCUS       DACDYM010000085         9640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDYM010000085 DACDYM010000000
VERSION     DACDYM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.086"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..2271
                     /locus_tag="IWM59_002286"
     CDS             82..2271
                     /locus_tag="IWM59_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9980282.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2268..4061
                     /locus_tag="IWM59_002287"
     CDS             2268..4061
                     /locus_tag="IWM59_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9980283.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            4261..4575
                     /locus_tag="IWM59_002288"
     CDS             4261..4575
                     /locus_tag="IWM59_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9980284.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            4596..4973
                     /locus_tag="IWM59_002289"
     CDS             4596..4973
                     /locus_tag="IWM59_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9980285.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            4983..5756
                     /locus_tag="IWM59_002290"
     CDS             4983..5756
                     /locus_tag="IWM59_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980286.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            5778..7181
                     /locus_tag="IWM59_002291"
     CDS             5778..7181
                     /locus_tag="IWM59_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9980287.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            7363..8547
                     /locus_tag="IWM59_002292"
     CDS             7363..8547
                     /locus_tag="IWM59_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9980288.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            8544..8834
                     /locus_tag="IWM59_002293"
     CDS             8544..8834
                     /locus_tag="IWM59_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980289.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            8831..9052
                     /locus_tag="IWM59_002294"
     CDS             8831..9052
                     /locus_tag="IWM59_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980290.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            9094..>9640
                     /locus_tag="IWM59_002295"
     CDS             9094..>9640
                     /locus_tag="IWM59_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980291.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKL"
BASE COUNT         3421 a         1473 c         1775 g         2971 t
ORIGIN      
        1 tataatgaga acttttacta acataattta tgataaactt actatataat catattaatt
       61 aaatagttag gagatctgct tatgtttata tctactcttc aaattcgaaa ttttagaaat
      121 tatgaatccg aaatctttcg attcgataat gagacaaata ctattatagg tgagaatgat
      181 tcagggaaaa ccaatgctct tactgcattg agaatcttat tagatgatag ttattactat
      241 agtagtaaaa cactaaaaga atcagatttt tttcatggaa ttcaaaatgg gtggcaaggg
      301 cattggatta ttatctctgc gacatttgaa ggaatcagcg aagaagaatt tgacaatgag
      361 atttgtgctt ccatttcttt aaattctgaa agtcaaacaa tcttagaaga attgatttca
      421 aatgcagata aaggtgtagg tagtatttct ctatttatta gacctaacaa agctattaga
      481 aagcaattat ttgatatttc cactgaatta gactctcatc aatttaacga atttagaaat
      541 tctattcgtc tatccgacta tgaattttac tacacatcta aatcaaatct aaatttctgt
      601 atcaatgaaa attatgataa attggtagga agactcaatg acaatatagc tagtaatcct
      661 gaagaagacg atgaagctct gttgggctca agaattgaca tgtcagatat tttcaagcat
      721 atttcggttg tttatgttga cgctcttcga gatgttctta gagaaatgaa aaacaatcga
      781 aacccagtca aaagaatcat ggaaacaatt gaatcaaaaa tatcatcaga taatgttgag
      841 tcattaaaaa caattataca gcaattaaat gagactatta ccagtgttcc tgaaattagg
      901 aaaattggcg aaaatattaa tcggcaacta aatagcataa ttggttcagt gtattctcct
      961 aatcttcttc tttcctcaac tatgtcagat gatatgggtt ctttagcaaa atttctttca
     1021 atgaagccag aacaaaatat cgacttagac ttactagggt taggacatct aaatatgatt
     1081 tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact acttaatatt
     1141 atgttgattg aagaaccaga agctcatatt catcatcata ttcaaaaaac tctgttcgaa
     1201 ggattaaatc ttcaaaaaaa ctatactcag attctcatga ccacacattc tgtacattta
     1261 gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg caaatctatc
     1321 gttatgaatc cagcaaataa tctgaactca tttgctaaag aaaagttaaa aaagaacaat
     1381 ctaaatctaa taaacgcagt agagagatat ttagatgtta aaagaaatgg attgcttttt
     1441 tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa tacttattcc tcaaatagta
     1501 aaaaaattat ttggtataaa cttagacgaa cttggaatag gtttgattaa tgttggtagt
     1561 acttcgtttg aatatatcgc tagcttgttt gacgatgata gaatacagag aaagtgtgct
     1621 attgtaactg atttagatga acaggcaatc ccatcagaac atcgattgta taaaagcact
     1681 gcacaagaga aaggtcaagc aagaaaagaa aaattatcct ccctttttaa tgaaaacaat
     1741 tgggttaatt gtttttatgc taatacaaca tttgaaattg agtttttaga tgtgaataaa
     1801 gaaaatattc taacttatgt cagtccaatt atagatgacg cattcaaaga taaaaaaaca
     1861 ataactgctt ataaagaaga aatacaaagt gaacaatatt tagaaagaaa tttagcgatg
     1921 ttaaaactag ccaatcatgt tggtaagggg tggtttgcaa ttgagttagg tgaaaaaatt
     1981 gactttttaa tcaaaattcc taagtatatt cttaatgcta ttgcttttgc atgtcaggaa
     2041 atagtagatc tctcaattta taaaaaaatt ctactccata caatcgactt gtacgaggaa
     2101 actacagaat tgtcagcttt atcagctagt ttaaaaacaa gtgatacagc tgatttgaat
     2161 atagcaataa tttctttatt agagtacttt ggtaaagatg aggatgttgt gtatctaatc
     2221 aatcagatag aaaattacac aaaaatactt tatgctgagg tttcaaaatg acaaatatct
     2281 tcgaaaaaat cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa agtgaagata
     2341 acctttatgt aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca aggaaaattg
     2401 cttaccaagc aattaaacac gatggatcac taaaaagaat aattgctatc acatacacta
     2461 atagagctgc tgaagaaatt aaggaacgcc taagtttact tagtatagat gatgatatta
     2521 atatttgggt ggggacaata catcagtttt gtcttgaatt tattatttat ccttttaaaa
     2581 tgaatctctc aagagtatca aaaggattta caattattga tgattatact caaagagctt
     2641 acgtcagcca aattaataat atgctgaatt taaatctcaa aacgtatgaa ctgaacaaaa
     2701 ttgatttgtc actttctcat gattttaaaa ttaatgaagt tcagtatact caagtttctg
     2761 aaatgtatca cactatgttg gaaaaaaata aagaaattga ttttgatatg attttactag
     2821 tagcgtataa aattttaact gtaaacagta acgttgcacg taatattgct agtaatataa
     2881 gggcgattta tgtggatgaa tttcaagata ctagagaatt acaatacaat gtaatagcta
     2941 aattacttca aaacaaccct caaattcagt caatgtttgt tggagatatt gaccaagcaa
     3001 tttatggatc attagatggt atagctaaat cagttgaaga actagaaatg ctaactagtc
     3061 acacttttca atcaaagacg ctacatggct gttacaggtc taatcagaga ttagtagact
     3121 tctactctaa ttttcagagt tgtccttacg agattgaaag cagagggaat aataaaaacg
     3181 accgtggttt tataagttat gattgcaaag ttactaagga ggatttaccc aacataataa
     3241 aaaatataat aaaggaaaag attgaaagcg gcattcatga aaaagatatt tgtgtacttg
     3301 ctcctcaaca ctatcctctg ttttcactgg gtgagtattt aaagaaagaa ttaccgtatt
     3361 gcaattttga tgctataaat atatcaccaa ttaaggtaaa taatcatagt ttatttttca
     3421 agttagcaat tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga aagctaattg
     3481 caaacgatat tttaattata ttaaaaaatg aatttggaat agaagttaaa gattatttta
     3541 ttgacttaga tttattacag ctaataaaca gtgtgaaaag aatgtatagt ggagacgaca
     3601 acggtgttga tttatacaaa tttgttacca aacatatatt tgttaatctt aatattgacg
     3661 agaaaaatta cccgtctatt tttaagcact atctattttt tattaacgaa gtagaagatc
     3721 ggataaaaaa taattcatta tctagtagtg tatctagttt taagaagatg tttaaatctc
     3781 gagatggtat cacacttact acagcacata aagttaaagg aaatgagtat gatactgtta
     3841 tcgctattaa tcttctaaac gggaaaattc ctcattggaa tgaaattttt aatgtttctg
     3901 ataaaggaat tagttcagcg agaaaactac tatacgtaat ttgttcaaga gcaaaaaata
     3961 atttatttct tttctcagag gtgggttatt ttacgagtaa aggagacttg ctttatccca
     4021 ctcaattatt agataaaata gaatttgatt ataatattta aaatacctag gtagtcgttg
     4081 ttttaaatat agtaggaatc ggacgctcag tacggagcgt ctattttttt acccaatttt
     4141 gaaaggaagt gaacttatga aaataaaaaa tcaagaatca aaaggtcgct ctcccccctt
     4201 taaaaccatc acacaaataa gattcattca aacataaaaa cagaaaggat aggtaaaaat
     4261 atggaactta aatatgtcat tcccaatatg gaaaaaacat ttggacaatt agaatatgct
     4321 ggtgaaggga acattgaaca acgtcgaata aatggcagac caaccattct ctctcgtagt
     4381 tacaatttat actcaacgat tcaacgagca gatgatattg ttgtcattct tccctccgaa
     4441 gctggcgaga aacattttga atccgacgaa aaagtcaaac tcatcaaccc acatattacc
     4501 gcagaaggtt ataaaattgg caatcgagga tttaccaatt acattatgca cgcagacgat
     4561 atggtgaaag cataagaaag gaaaggtgaa aaaatatgag attagcagaa ggtattgtga
     4621 ttgaaaaaga ccagacattt ggcacattga aattttccgc cttgcgtcgt gaggttcgta
     4681 tccagaatga agatggtacg gtttccgaag aaattaaaga acgtacctat gacttaaaat
     4741 caaaaggaca aggacgcatg atacaagtca gtatccccgc ttctgttcct ttgaaagaat
     4801 ttgactacaa cgcagaagtg gaattaatca atcctgtggc agataccgta gcgacggcta
     4861 cttttcgagg ggcagacgtt gattggtacg tgaaagcaga cgatcttgta ttaaaaggac
     4921 agacaaaacc tcatcagaaa tataaaaaag aagcgtccga aagtgaaaaa taggtgttgc
     4981 ttatgacaaa cacaccattg attttgaaag aaattccgaa ggatgaagcc atttctttta
     5041 ttcggcaata ccattattca aagatactcc ctagattgtg taaatacttt ctagggattt
     5101 tttcagaaga aaaattatta ggcgttgtgg aattaggctg gggtacgcaa cccctacaaa
     5161 ccatacgcaa actctttccc gacagttccc ttcaaaccac agattattta gaaattggaa
     5221 agatgtgttt tcttccagaa atgaatcaaa ccaattattt tggcagtcaa gctctttcag
     5281 ctctaattaa gtggttgaaa gaacatacgg actgtcattt tttatataca ctggcagatg
     5341 gaattgaggg aaaatgtggc tatgtctatc aagcgtccaa cttcttttac tgcgggtatt
     5401 tcaagacaag tgtctatcgt gataagcagt catgggaaaa gatacaccct cgaagtgctc
     5461 gccttttatt agaggaaaat gctcgctttg aacaggtaga gaaaaagcat tggctttccc
     5521 aggcattttg cgaatacaaa gggattgaga aaatcaatgg acgcatgttt cgctatctct
     5581 atccgttgac aaaagaagcg aaaaaactgt taggacacac tctctatcga cgtcattatt
     5641 atccgaaaga aaaaaattta cgctttgaaa agcgaatcgc ttatcagaaa tatgaagcca
     5701 tttctcaacc aacttttgat aaacaggctc gtatctacaa tacacaatta ttttgatagg
     5761 tggaaaggag gatttttatg ccattgcttc atgacagagg agagcgaatc aaagccaacg
     5821 ataagcactt ggtctatcat tttgtactaa tttcattgct ggtagttttt ctagtcgtcc
     5881 tactcccttt tcattggaga gcgttacaat cggtgaactg gcaagcagtt cagttagata
     5941 catttttcta tcaacttcat acgccatact tttggagcag tattctcact gctctttttg
     6001 tatgcggact tatcagctgg ctttattatc actatcgtat agattctata aaacagttag
     6061 agcaccgtca aaagttagct cgtatgattt tagaaaatca ttggtatgaa gccgaagaaa
     6121 ctcaatcaga gggattcttt aaagatttac ccagcaagaa aaccaaacaa cgaatccgcc
     6181 attttcctaa aatatattat cgaatgaaag atggactaat ccatattcat gtagaaatca
     6241 ccttagggaa atatcaagat cagcttttgc acctagaaaa caaactagaa acaggattgt
     6301 attgtgaatt agtggaaaaa acgttgaaag attcttatgt agagtatgtc ctactttatg
     6361 atactattgg caatcgaatt ggcattgaag acgtggtagc tcaagatgga cgtgtcaaat
     6421 tgatggaatc ggtctattgg gagttcgatt ctctccctca catgttaatt gctgggggca
     6481 ctggtagtgg aaaatcctat tttattctca cattgattga atctctcctg catacaaatg
     6541 caaagcttta tattcttgac cctaaaaatg ctgatttggc agacttaggg gcggtcatga
     6601 ataatgtcta ttaccgtaaa gaagatatgt tggcatgtat caatcaattt tacgaagata
     6661 tgattgatcg cagtgaaacc atgaaacaac accctaacta taaaacagga gaaaattatg
     6721 cttatcttgg tttgtctgcc aattttctta tttttgatga atatgtggca atgatggata
     6781 tgctaggtcg tgaaagtacc accgttattc ataaactcaa acaaattgta atgttaggac
     6841 gacaagcagg cttcttttta attcttgcct gtcaacgtcc agacgcaaaa tattttagtg
     6901 atgggattcg ggacaacttt aacttccgtg tggctttggg tcgtatgtct gaaatgggct
     6961 ttggtatgat gttcggaagc gatacacaaa aacaattttt cttaaaaccc atcaagggaa
     7021 gaggttacgt ggatacagga aaaagtgtga ttagtgagtt ttatacaccc cttgtaccaa
     7081 aaaggtacga tttcttagga gaaataggaa aagtcattca aaaaaagcaa tctgagcctg
     7141 taccgcacga agtgaaaggt acaggttcag acttgctttg attggtgtgg cgttagccac
     7201 gccaatcctt tagccccctc tatctaacag gggggcacaa atacaactga aaacaggcaa
     7261 ttctcatcaa ccaatgttga tggtacaaag gattgcggaa aatatgaaag gtgtaaactc
     7321 aagggtcata gtttacatct tttttagatt ggaggtttgc gaatggaaaa cgaaaaaatt
     7381 tggattaagg aattgaaaga aaagcgactg gcttatggcg tttctcaaaa taaactggcg
     7441 gttgcttctc atattaccag accttaccta tcggatattg aaacaggcaa ggcagtccct
     7501 actcaacagg tgaaagaaga tttactgaac gctttggaac gcttcaatcc cgataaccca
     7561 ttggagatgt tattcgacta tgtacggatt cgattcccta ccaatgatgt ggctcaaatt
     7621 atcggtgagg ttttacgttt gaatatggat tatatgctac acgaagactt tggctattat
     7681 tcttatcccg aacactatcg tttcggtgat attgttgtcc tagtctctca tgatgtaagt
     7741 aaaggtgttt tattagaact gaaaggaaaa ggctgtcggc aatttgaaaa ctttctattg
     7801 gctcaacatc ggagctggta tgatttcttt caagattgca tggaacataa aggtattttc
     7861 aaacgcttgg atttagctat caacgataaa acagggattt tgaatattcc agatttggca
     7921 aagaaatgta aacaggaaga atgcatttcc gtctttcgaa gctttaaaaa ttatcgcagt
     7981 ggtgaattgg ttcaccgtga tgaaaaacca tacatgggaa acacacttta tattggctca
     8041 ttaaaaagtg aagtctattt ctgtatatat gagaaagatt atgagcaata cgtgaaaaat
     8101 gatgttccct tagaagacgc agaagtaaag aatcggtttg aaattcggtt aaaaaatgac
     8161 cgagccaccc aagccatgaa agatttattg gcacatcaac aggcggaaaa aacagccttt
     8221 gaaattatca atcgctatat ccgctttgca gataaagacg acactaaacg tcggagtgat
     8281 tggaaaacta atgaacgttg ggaatggttt atcggtaaaa atcgtggagc attacgccta
     8341 accacacagc cagagcctta ttcttttgaa cgaacattga actggttaca tcatcaagtc
     8401 gcccctacat taaaaatcgc aagcatttta gatgttctca acggtacaac cattatctcc
     8461 accatgattc aggaagccaa actgacagaa aaacatgaga aattaattga acaacaacat
     8521 ctagccatgg aggatttgat tacatgagcc ttttaacaaa agaattaaag aagttagggt
     8581 ttcagtgtgg cattgagttt caagcgtata tacaaaatac agggaaatat acgtctctca
     8641 ttattgaggg aaaacggcaa gctggtgata ccatctatac ctatgatttt tacaaggtga
     8701 gattttataa taattatacc aatcgagtaa ccgtctatgg agagcattta accccttttc
     8761 aattattaag gcgtgtaaaa agctatatct attatcgaga aaaatatctt aaggaaagga
     8821 ggacaatcac atgaattttg ggcagaattt gtacaactgg ttcttggata atgcacagtc
     8881 attggtattg ttagcgattg ttgtcattgg actgttttta ggttttaaac gagaattttc
     8941 aaaactcatt ggcttcttga ttattgctct aatcgccgtc ggacttgtgt tcaatgcagc
     9001 tggcgtgaaa gatgtgttat taaacttatt taaccgaatc attggtgctt aacagatatt
     9061 tttcatgata aaataggaag tatatatttt gttatgaaag gatttatgaa tatgtttaca
     9121 aatgatcaat ttaaatctct tttaattaaa acattagaaa gaaagagctc ttcaaattat
     9181 tatgaaggag gtaacgagat tgtttctaaa atgaaaatat cagaggtaac tttagatgag
     9241 actgacgatt ttacttatta taaaggatat aaaaagggct ggaatacgct ttgcacatat
     9301 cttaaaataa gagttccttt tgatgattta gacttttttg aaagtcataa agatttgatt
     9361 acccagacag caagctccat atatgataaa caaggtgata atgtattagt ggatactatt
     9421 ttagtaccac ttccagagaa ttacgaagta attaacttta gtcagttgaa aattagtgat
     9481 gtggtttcac aagctattga agacgctgag tcttttatga gtaatggtga atatcaacgt
     9541 gcctttgaca gagttcacac tgcttttcat ggatatttaa ttgaaatact aaaaaaatat
     9601 gaaattacag ttcctagaga cgaaaatcta tctaaattat
//
LOCUS       DACDYM010000086         9279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDYM010000086 DACDYM010000000
VERSION     DACDYM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.087"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..954
                     /gene="ccpA"
                     /locus_tag="IWM59_002296"
     CDS             <1..954
                     /gene="ccpA"
                     /locus_tag="IWM59_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9980292.1"
                     /translation="VSMATVSRVVNGNPNVKPATRKKVLEVIDRLDYRPNAVARGLAS
                     KKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSDGNDQKEVNVLNNLLAK
                     QVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSVNIDYVSATKDAVNKLA
                     KSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGLIFEAQYNFKDGLSLVD
                     RIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSNNSLLTEVARPRLTSIT
                     QPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            1308..1703
                     /locus_tag="IWM59_002297"
     CDS             1308..1703
                     /locus_tag="IWM59_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980293.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            1700..2956
                     /locus_tag="IWM59_002298"
     CDS             1700..2956
                     /locus_tag="IWM59_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP9980294.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            2968..3291
                     /locus_tag="IWM59_002299"
     CDS             2968..3291
                     /locus_tag="IWM59_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980295.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            3284..3550
                     /locus_tag="IWM59_002300"
     CDS             3284..3550
                     /locus_tag="IWM59_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980296.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(3713..6058)
                     /locus_tag="IWM59_002301"
     CDS             complement(3713..6058)
                     /locus_tag="IWM59_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9980297.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            6337..7239
                     /locus_tag="IWM59_002302"
     CDS             6337..7239
                     /locus_tag="IWM59_002302"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9980298.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            7450..9225
                     /locus_tag="IWM59_002303"
     CDS             7450..9225
                     /locus_tag="IWM59_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9980299.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
BASE COUNT         3031 a         1689 c         1873 g         2686 t
ORIGIN      
        1 gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgtcaa accagcaaca
       61 agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaatgc agtcgcacgt
      121 ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca ttcctgatgt cagcaatatg
      181 ttctttgcct ctttagcacg tggtatcgac gatgtggcaa caatgtacaa atacaatatc
      241 atcttagcaa actcagatgg caacgatcaa aaagaagtca atgtattgaa taatttattg
      301 gctaagcaag tggacggcgt gatctttatg ggtcaccata tcacagacga aatccgtgga
      361 gaattttcac gttcaaaaac gcctgttgta ctagctggtt ccattgatcc agatgaacaa
      421 gtaggtagcg taaacattga ttatgtatca gcaacaaaag atgcagtaaa taaattagca
      481 aaaagcggca ataaaaaaat cgcttttgtc agcggtgcgc tgattgatcc tatcaatggt
      541 caaaaccgtc taaaaggcta taaagaagca ttaagcgaaa acaatctacc atatagcgaa
      601 ggattgattt ttgaggcaca atacaacttt aaagatggtc tgtctctagt tgaccggatc
      661 cataatagcg gtgcaacagc tgtctatgtt tcagacgatg aattagccat tggtatcttg
      721 gatggtttat tagaccgcgg cgtaaaagta ccagaagaat tcgaaatcat cacaagcaac
      781 aattctttac tgacagaagt agctcgtcct cgtttgacaa gtatcacaca accattatac
      841 gatttaggag cggtatccat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa
      901 gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatcaactaa ataataaaag
      961 atcaatcatt taaaggttgt tcccaaagtg gtcaacttct ggaacaacct ttatacgaga
     1021 aaaggggtta tgacaaaatt ttgtcataac cccttttttt gtgactttaa aaaaaagaga
     1081 gaatggcgga aacaaacctt acgtccatcg tatacgacac aatctgtgcg tagatgctac
     1141 attttgttca ctactatcaa aattacttta aaatgaaaac gtgataaaaa agaaagtaga
     1201 aaagtagaaa agtaactcta aaaaatccaa aaaaatccag gctcgattgc gtacttatct
     1261 taaaggagaa acaatccatc atacataacc cgaaaggaga cttctatttg cctaagaaaa
     1321 gacgaaagac catagtcatc attttgacca ttcttactat aggaatcgga ggaaaatttt
     1381 acatggacaa aagagaagcg caaaaacagc aagatttact cgcagtagaa aaacagtccg
     1441 tcaaagttct gaaaaatacc tttgcggata tcaaagaagt gaaaatcgaa caatttgaga
     1501 aaaacgacat gacaggaatc tatgaagcat tgataacgat gaccaataca gaaggaaaag
     1561 ccgtttattt tgattatagc tttacaaaag aaagcaatga gatagatggc tatggtgttg
     1621 aaaatgaaga agtacaaaaa gaagggatta cggttgatga gattatagtt atctatacag
     1681 atgggagtga ggaaaaagta tgacaattac tgataagcag tataaaggaa taagtaataa
     1741 agtatattgg ctagatccaa aacatcctca atatagtaaa agttacaaag aaggcgccat
     1801 taaaaacttt ggagaaacaa aattccaaat cctaaaaatc aaaaactcct tcgacggcat
     1861 gcaggcgatg gctgtagcgc caatcaagaa cggtaaagtg gatatttctc aagtagtgat
     1921 tgcttttgca ggaacgaact taagcgattg gaaagatttg gaaacggatg caagaagtgt
     1981 tatgaatctc tatgggaata gaccaggagc aagtttttct gaaaaagcaa caacaaacaa
     2041 gattttatcc gttagtggat tgaaaacagc ggggcaaatc aattcagcga ttgcatttgc
     2101 taatgaagta aaaaaggaat atggagaagc tgctatttct actaccggcc attcattagg
     2161 tggattctta gcattatatg tagcagcaga gaaacaatgg aaaaatgtcg gcttcaacgg
     2221 gccggacccg tatgatctct tatcaccaga agcaaaaaaa tgggtggaag aaaatcccgg
     2281 tatgctcacc aattaccgaa atcacgccga ttttatcggt aattttggtg gtaatggcac
     2341 aggtgctgaa gttcggatca gtatggaaac agcatacttg aatctgcttg cttcccacag
     2401 tttaacctct tggaagtttg atgaaaatgg aaagctgatt attcctgaaa atgaaaacaa
     2461 tgttaaagct cgaaaacaac agaaagaaaa ccaagcgacc cagttgtttt cctatcagtt
     2521 gcaagaactg aacaacatac gtaaaaaact acgattaagt ggcggccggc tgacagttaa
     2581 tgaacaaatt tacttagatg acacacaagc taggttagtg gtggaaaagt tatcggtaaa
     2641 aatgaaaagc gctatggaag aagttatccg catcaaccaa agaggaattt ttgagctaga
     2701 gaaaaaatgg caagaaggct tgcggcaagc acgtgttgtc gccccttcgt tgactgaaca
     2761 ggaaatcata gaagcattag ctagtgtggg cgccacaaaa gagaacttag tcgattctgc
     2821 gaaagaagtc tatctgctac gaatcaataa ggcaagacgt atgggggagg cctttgatac
     2881 attggcgaag gagatccaag acaaaatcaa tgaaatcgtg gcacgagatc gagaattagc
     2941 acagcaattc aattaaagga gaagtagatg aaactagaag aattggaaag agattatcga
     3001 agtgaacgaa gacaattaga agctcaagaa gatgaattga tccactttaa gaatcaagga
     3061 ttacgattga tggagcaaga ggagcagcga ctgcatcatg tattaaagaa cttagcttta
     3121 gataatgagc ctctgtttca agcaagacga agttattcga attatcagga gcattatcaa
     3181 caagccctac aagtagaacg acgaaaactc tatcagcagc aagacgaact agaacgttcc
     3241 tatcgtcaaa aaatcagaca aataactatg gagaaatcat cttatgaatg aatttttaat
     3301 gcaattacta aaagaattag agaaaaatct acatcgatca aatgtacgtt cagttggtat
     3361 ggggatatct cagcaaactt gggcagaaaa acgattgaca gatcaaaacc accaaatgac
     3421 tgcagaagaa gctactcaag aattttctaa ccgacatgca cacttaaaag aatcagtaaa
     3481 agggcaattt gcagaaaaac taaccgaatt attccaaaga caagaaaaga aattaaaaca
     3541 gtttcgttag taaagttata tagaaaaatt ttgtgaaaat ggtttttcac atttttacat
     3601 aaatttgtct taatatcgta gatgtaagct tttgaacacc atttattcgg aaaaagggag
     3661 ctgtgacaag atttttgtcg cagctccctt taattctgat taatctatta gatcaatcat
     3721 ctttatcgtc tgattctttg gtctctggat tcgctctagc ataagctgaa gcggtgttcc
     3781 agtaatctgt atagttttca tcagtaccgc caacgccgaa tttaaaggcg cttttcttcg
     3841 gaccattttt tgcccaaagt gattccactg tttttcctgg cgtttggata gattttttat
     3901 tgacagtaac ttttccagct gtttgtcctg tgaacccaga tacttttttc ttcatcacat
     3961 cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc agggtttgct tggtaagctt
     4021 gattgattag attggcaaga taattagccg aacgtattct tgcttggctg tccattccag
     4081 tattgtcatc gtgtcctgcc caactactga tagtgataga tggagttgag acgatcagcc
     4141 atgaatctcg gtattcattg gtagaaccag tttttccgac ccaatctgca tttcctagat
     4201 ttccgtttaa gcttgagatg acatctttga atggtgtagt gattttttca ttgatcacac
     4261 tacgcatcat gtcattcatg atcgatgccg ttgcttcgga ataaatacgg acagaattac
     4321 tttcatgttt ataaatcaca ttacctgcat tatctgtgat ggaatcaatg atatagcctt
     4381 cttcgtagac tccaccattt gctaaggcct ggaatccatt tgtctgttga agcgtcgtta
     4441 catctactgt ccctaaagga gccgattcaa cgccccaatt atcatttgct gggtagttca
     4501 tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc accggaattc aacacatctt
     4561 gatataagtg ataagctggg atattgttgg accattccag tgactcacgg acggtttgga
     4621 aggtgttcga cccttcattg gtcgcattga cgattttctc accagcattt tttccttctt
     4681 gccaagtcgt tgcataatct gatacacggg attctgatcc gacaagtccc atatcgattg
     4741 caggaccgta aacaagaacc ggtttgatgg atgaaccagc ttgacgttct gtatcaaagg
     4801 cgtggttatt ttgattctgg ctgtaattcc gaccacccac gaagccatag atacgtccgg
     4861 ttctgttatc catcaataca tttccagtct ccacttggct accagcacca tcatctaaca
     4921 tgtagccata attttctacg ccggtttgca atgcagcata gatatcttta tcaatcgtcg
     4981 aatggatcgt atatcctttg ttttggatcg tttgctgtgc tttttgataa taagcttcac
     5041 tgaccgatga atctgacata tcagcattat ctttttctgc caattgttgt gcaataatcc
     5101 ttgttgcttc gttcatcacg gtataataca agaattctct gtcagtctgt tccgcgattt
     5161 gttgtgggag aaagtctttt gtaagatcat aggctttcgc ttcttcgtat tgttctttcg
     5221 taatttgtcc ttcacggtac atactgaata aaacaaaatc ttttctggca agaccggcag
     5281 aaagatcttc ttttagcgcg cctgtattcg tgtatggact atacgtgata gggctttgag
     5341 gcaaaccagc gatataagca gcttgcggca gagtcacatc ctttgcatct acaccaaaga
     5401 ttcctctagc agcttcctgg actccagcaa tattttgccc cttattattc cgaccaaatg
     5461 gtgagacatt caaatacgta gtaatgatct catcttttga aaagtgtttt tctacttgtg
     5521 cagaaagaac gatctcgttt gctttacgtt tgaatgttgt ttcatcggtt aggatctgtt
     5581 gtttgactaa ttgctgtgtc aaagtggatc ctccagacga gccgatgcca gtcgcttctg
     5641 aaactagtgc tcgtaaaaca gctttaggga cataaccttg gtgtttatcg aagtattcat
     5701 cttctgtact gataatcgcg gttttcaata aaggcgaaat cttatcagat gaaatagtcg
     5761 tccgcatcag atctgtctgg atttttgata tctctgtatt atctgcataa acaatctttg
     5821 acgtttccgt aatgtttcct agttctgtct gcagttcttt tttagtcggc aattgtgtgt
     5881 cttcgacaag ataggcaaag tagccagcac ctattccgat acctagagct gcgaacaaca
     5941 aaagaagaac agtaataaaa acagtcaatg attgaaacac tctaaaaata atcttaggaa
     6001 caaaccattt ttcttttttt cttctattct gtttttttcg agtatttttg cgtggcaaaa
     6061 gggtcacctc aactaatggt tttatttcgc taattatagc aaataatagg acaaaaaaac
     6121 agacaaggct gtttttgaca ggaatttctt tttcgaatga gataaaacgt gaggaaatga
     6181 tcttttttat gaaagagttc ttaagctttt ctttagggaa agtttcagtt actatggaga
     6241 acagggcaaa gaaaaacaaa ctattttctt tgtacatcat cttatcaata tacaggaatt
     6301 gttcgaaaca attgcctttc aaggaggaat ccatccatgt tattaggctc acacgtcagc
     6361 atgagcggaa agaaaatgct gttaggcgcg gcagaagaag cagccagtta cggatcttcg
     6421 acctttatga tctatacagg cgctcctcaa aatactagaa gaaaagctat cgaagagatg
     6481 aatattgatg ccggaaaagc atttatggca gaaaacggac taacaaatat cgtcgttcat
     6541 gcgccttata tcattaattt gggaaatacg atcaaaccag aaatgtttcc atttgctgta
     6601 caatttctga gagaagagat tgtccgtgcg gaagctttag gtgctactca aatcaccctt
     6661 cacccaggcg ctcatgtagg agcaggggca gaagctggga ttgctagaat tattgagggg
     6721 ttgaatgaag ttttgacaaa agaacagacg gcacagatcg ctttggagac gatggcaggg
     6781 aaaggtacgg aaatcggtcg aacatttgag gaattagcgc aaatcatcga tggagtaaaa
     6841 ttgaatgaaa aattatccgt tacttttgat acatgtcata cgaatgatgc cggatataat
     6901 ataaaagaag atttttcagg tgtaatggaa gagtttgata aggtcatcgg attagatcgg
     6961 cttaaagtac tgcatatcaa tgattctaaa aatcctcaag gttctcataa agatcggcat
     7021 gccaatatcg gttttggaac aattggattt gacacattga acaaaatcgt ccatgatcca
     7081 atgtttgaca acatttcgaa aatattagaa accccatatg tcggtaaaga taaaaagaaa
     7141 gcttacgcac catatggaaa agaaatttcc atgttcaaag cgcaagcatt cgatcccgaa
     7201 gtgttccctg agttattagc agaagaagtc acactttaag ttttctacct gacagcaaca
     7261 aaaaaggatt gggcagtctg ccttgtcctt ttttgtttaa gaaacaaagg agtgcttttt
     7321 gcattcgttt tcgccatgct ccttttgccc cctcttttta aattcagaca aacagggtat
     7381 actgatagaa aagtaaaaaa cgaaaagttt cacaaacaag tagcaaaacg tgattgaaag
     7441 gacgaaaaga tgctgaacat tttaaacccc ttgatcgacc aactaaaaaa gaaacataca
     7501 tactttattc ttggcatagc cgtattattt acaattattc ggttgtattt atatagtaaa
     7561 gcaatctatg gaacagatat gtcggcgacc tatgatgatc agctgctgat tcactatgct
     7621 caaaatattt tggatggtaa atggctaggg gagtacacga ctacgacact ttcaaaaggg
     7681 atttcctaca gcttgttttt agtacttgca cataaactgc atctttctta tgcagtttta
     7741 ttcggcatgt tcagtattct tgccagtata ttggttgcac tagcgtttaa accaatcgtg
     7801 aagaacaagt atcttctttt aagtatctat ttttattttt tgttttctcc gattagtttt
     7861 acacatgaat attccactcg aacatatcgt aatgctctcg tgatcccgac tgttgagatc
     7921 attatcgctt gtttactagc tatttattac cgcaaatata attccagcaa aaaattgctg
     7981 ccgtggtttt tagggctagc tgtttctttt cctttctttt ggttcattcg cgaagattcc
     8041 atctggcttc ttccttttac tgtagtcgcg ttaggcatca gtttcgttca gatcttgcta
     8101 ggacaagact tttctttcaa agaagcaaag aagttaaaag aacgaccatt tctcctttcg
     8161 aaacaagaat ggctgaaact ttcattgttt ttactccctc tagtcggttt ctccatcgtc
     8221 tatcagtgga tcgaatataa aaacgagaca acatatggta ttgcagccat caatgaccgt
     8281 tcgagtggcg aatttggacg tttgatgaaa cagctgatcc gaatagatga cgaaacaaac
     8341 ttaaataaag aaaacagtga gatatgggtc tcgcatgctg cattaaataa agctttagac
     8401 gcatctccta catttcatac tgtttctact cgtattgacg aattgtatac gactcatgcg
     8461 tggacccaag gcggaaaagt aaaagaatta ccaggagata tcattttttg ggcattgcga
     8521 gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg
     8581 aaaaaagtca atcaagaatt agaagaagcc tatcaaaagg gaactatcaa aaagaaaaaa
     8641 gaattctatc tgatgggatc aggggatgga aagacgatga atgatctgcc aatcgtattt
     8701 gattttctga aatcaggata tgcttatgca tggcgataca aagatttcca tcaagggggg
     8761 aattatagtt ttgggacgca agaacaagtc gaacaggcac aagaattatt gaatcttcca
     8821 ctaatggata attggatcga tagcagtgat ccgagaacac ctgatttcca gttgacaaaa
     8881 acagcaaaag tcgcgaatgt catgataaaa atttaccaag ttctggccat tcccgtgctg
     8941 attgcttcaa tagcagggat attacttatg atactaggaa ccgtatttgc aaaagctgat
     9001 cgtgcctatt atagttcctc tttgattgta gtcatcggat taatactgac acaattggtt
     9061 tttcttttcg gtgtctcgtg gttttgttct tatgcgccag aaaaaaagga ttatttttta
     9121 agtgtctaca ctggcgcagg cgtgccatta acgcaattca tcgcagtaac aagcatgcta
     9181 gtttttagta aattgaatct gaaaaaagct ttatcttcca aataaaaagg gaactgtgac
     9241 aaaagtcttg tcgcagttcc tttttccgaa taaacggtg
//
LOCUS       DACDYM010000087         9051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDYM010000087 DACDYM010000000
VERSION     DACDYM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.088"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            168..986
                     /gene="purR"
                     /locus_tag="IWM59_002304"
     CDS             168..986
                     /gene="purR"
                     /locus_tag="IWM59_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9980300.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1489..2862
                     /gene="glmU"
                     /locus_tag="IWM59_002305"
     CDS             1489..2862
                     /gene="glmU"
                     /locus_tag="IWM59_002305"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9980301.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2956..3927
                     /locus_tag="IWM59_002306"
     CDS             2956..3927
                     /locus_tag="IWM59_002306"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9980302.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3933..4035
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4101..4670
                     /gene="thiT"
                     /locus_tag="IWM59_002307"
     CDS             4101..4670
                     /gene="thiT"
                     /locus_tag="IWM59_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9980303.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4751..5524)
                     /locus_tag="IWM59_002308"
     CDS             complement(4751..5524)
                     /locus_tag="IWM59_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980304.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5791..7491
                     /locus_tag="IWM59_002309"
     CDS             5791..7491
                     /locus_tag="IWM59_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9980305.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7461..7679
                     /locus_tag="IWM59_002310"
     CDS             7461..7679
                     /locus_tag="IWM59_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980306.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7699..8589
                     /locus_tag="IWM59_002311"
     CDS             7699..8589
                     /locus_tag="IWM59_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9980307.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8607..8891
                     /locus_tag="IWM59_002312"
     CDS             8607..8891
                     /locus_tag="IWM59_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980308.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3061 a         1542 c         1894 g         2554 t
ORIGIN      
        1 aattataaag aaaagaagcc ttataaaaac gaaaaatttt ttataggctt ctttttattt
       61 taataaatac cgaactattt tcttattttt tcataaaatg ctcgaatttt tgaagaaaaa
      121 cttatataat gtaaaacgat aaaatttctt aaaaggagtt ggaataggtg aaagtacgtc
      181 gcagtgaacg tctagttgac atgacacagt atttattgaa tcacccgcat gaattgattt
      241 ccttaacaag ctttgccgaa cgttatgaat ctgctaaatc ttctatcagt gaagatttgg
      301 gaatcatcaa gaaaacattt aaagaacgag ggtatggaac cttggaaaca atcccgggtg
      361 ctgcaggtgg tgtattgttc atccctgaaa tttcatatga agaggcgaaa aaatatattg
      421 aatctctagc agaacgtcta tcagaacaag accgtctgct tccaggagga tatgtttatc
      481 tctctgattt gttaggagaa cctgaactgc tgcgtcaagt tggaagaatc attgcttcta
      541 aataccttgg tgaaaaaatc gatgctgtaa tgactgtagc cacaaaaggc gtacctattg
      601 cccaagctgt gtcctattat ctgaatgcgc cattcgttat tgtacgccgg gactcaaaaa
      661 taacagaagg atcaacagta agtgtcaact atgtgtctgg atcttctgaa agaattgaaa
      721 agatggaact gtctaagaga agtttgaaac gtggttcacg tgtccttgta gtggatgatt
      781 tcatgaaagg cggcggaaca gtcaacggaa tgaagagctt gattgaagaa ttcgaatcag
      841 aacttgttgg catcactgta tttgccgaat caaaattcaa cggtcatcgc gccatcgatg
      901 attatacgtc actgctttat gtcaaagatg tagatactca aacgaaaaat attacggttg
      961 tgccaggaaa ttatttttca gaataagagg ttgtgaaaaa gctgcttagt ttcgagtagt
     1021 aagaaaaatt ttgtgaaaat ggctttccac attttttcat aaatttggct taataccgag
     1081 aaactggctt ttgaacaccg tttatctaga ggttgtgaaa tcagcgtcct gtcctgagta
     1141 ttagctcaat aaacggaaaa attgctcctc atattttgcc gtttattgag ctaataccga
     1201 aggtcagctg attgaaaaac gtttaatgaa gaggttgtaa gcctaccgtt taactctaag
     1261 tcacaagaaa cgatttcaaa aaacagcttt tcatgtttta tgagtagtgt gacttgtgac
     1321 cgaggagttg gcaggagagc accggttacc aaatagatag gcccgttatg gtctatctat
     1381 ttttttatcc agaaaacgct gggataaaag aaaatcactt gttttgttga aaaaatgggt
     1441 atactgggat aaactgaaat cagcaaacta tatttaggag tgacgcagtt ggacgcacgt
     1501 tatgcaatta ttttggcggc agggaaaggt acccgcatga agtcaaaatt atataaagtt
     1561 ttacatcccg tttctggtaa acctatggta gaacatatca ttaatcgggt cagtgaaacg
     1621 aaaccagatg aagtgattac gatcgttgga cacggagcag aacaagttaa agcacaacta
     1681 ggagaacgca gtaaatatgc gttacaagca gaacaattgg gaacaggaca tgctgttttg
     1741 caagctgctt ctttcttgga aggaaaaaaa ggaacaacgt tagttatcag tggcgataca
     1801 ccattattga ctactgaaac gctgaacaat ctttttgaat atcaccaagg aaaaaatgca
     1861 agtgcaacga tcttgacggc acaagcagaa aacccaacag gatatggacg aatcatccgt
     1921 gaccatattg ggatcgtaga aaaaatcgtg gaacaaaaag atgcaacacc agaagaagct
     1981 cttgtacaag aaatcaatac aggaacttac tgttttgaca acgaagcttt gttcgatgcg
     2041 ttaagcaaag ttggaacaaa caatgctcaa ggtgaatact atctaacaga tatcatcgaa
     2101 atcttaaaag aagaaggcca tacagttgct gcttatcaaa cggatgattt tgaagaatca
     2161 atgggagtca atgatcggat cgcgttagct aaagcaaatg agatcatgag aaagcgaatt
     2221 aaccagatgc atatggtcaa tggcgtaagc tttgtcgatt cagcaacgac atacatcgat
     2281 gcaggcgtag aaatcggtcc agacacattg atcgaagcag gtgtacaaat ccaaggaaat
     2341 acagtcattg gttctgattg tgtcatcggg tcacattcaa aaatcgtaga cagtaggatt
     2401 gaggatcacg tcgtcattga aaactcagtc attgaaagca gccatgtcaa gaaacatgca
     2461 gatgtgggac cttatgcaca tttacgacca aaagctgaaa ttggcgaaaa cgtccatatc
     2521 ggaaactttg tggaagtgaa aaatgctcaa atcggcaaag gaactaaagt cggtcacttg
     2581 acttatgttg gtgatgctac gctgggagaa gaaatcaatg tcggatgtgg tgttgtcttc
     2641 gtcaactatg atggaaagaa caaacatcat acgactgtcg gcgaccacag ctttattggt
     2701 tctagcacga atatcatcgg acctgtcgag gtagctaaaa acagttcgat tgctgccggg
     2761 tcaacaatta cagacaatat tccagaatat gcgctggcaa ttgcgagagc aagacaagtc
     2821 aataaagaag gatatgcaaa aaaactccct tatctaaact aagacttttg caaaatcact
     2881 tgatttattt aaaaaatatg actactattt attaggtaga attctagagt tgtgaaaaag
     2941 aaagtggagg tccccatgtc aaaacattac tttgacccaa gattaaaaat ttttgcgtta
     3001 aattccaatc gcccattagc agagaaaatt gcagcagcag tcggcgtcga gttaggaaaa
     3061 tcatctgtta ctcaatttag tgatggagaa attcaagtaa acattgaaga aagtattcgt
     3121 ggatcgcatg tctatgttat tcaatcaacg agcagcccag tcaatgataa cttgatggag
     3181 ttgctgatca tgattgacgc actgaaacga gcaagtgcaa aaacaatcaa cgtagtcatg
     3241 ccttattatg gctatgcgcg tcaagaccgt aaagcacgtg cacgggaacc aatcacagcg
     3301 aaattagtag caaacatgat tcaaaaagct ggtgcaacaa gaatgctgac tttggacctt
     3361 catgcagttc aaatccaagg tttctttgat attccagtcg atcatttgat gggtgcgcca
     3421 cttattgcca attatttctt agaaaaagga atcaaaggcg atgatgttgt cgttgtttca
     3481 ccagaccatg gtggtgtcac acgggcacgt aaattagcgg aatttttgaa atctccgatt
     3541 gcgatcatag acaaacgccg tccaaaagca aatgttgcag aagtaatgaa tatcattgga
     3601 caagtcaaag gcaaaacttg tgtactgatc gatgatatga tcgatacagc aggtacgatc
     3661 acattggctg ccaatgcttt gaaagaagct ggtgcaacta gtgtgtatgc ttcatgtaca
     3721 catccagtat tatccggccc tgctttacaa agaatagaag attcagctat cgagcgtcta
     3781 gttgttacag actcgatcta tcttccagaa gatcgtaaaa tcgacaaaat cgatgaagta
     3841 agtgttggcg gactaatggg agatgcaatc aaacgtatcc atgaaaacaa accagttagt
     3901 ccattgtttg aaacaaaaaa taaataataa ataaactcac aaaggggagt ccaattgggc
     3961 tgagattgaa tctatttcta aacccttcgt acctgtatcg gttatgcgag cgtaggaatt
     4021 gtgaataaac agctgtttga ctattttcga aagctgcttc ctcctttgtg acttttttat
     4081 tcaaaggagg aagttttttt atgggaaaaa atagagtttg ggtagaagga acgattgttg
     4141 cagcgttagc gatggtgttg tcgttgattc cgatccagat cggcagcagt ttttctattt
     4201 cattagggca gattccattg actctttttg cgttaagaag agggacaaag gcagggatgc
     4261 tggcaggttt catttggggc atcctacatt ttccgttggg acaagtttat tatttaagtg
     4321 tcgttcaagt gttgattgag tacccgattg cctatacttt tgcaggcgca gcagggctca
     4381 tggcttcaaa tgtccaaaaa ggctatgtag acgataatac gaaacaaatt agaaaaagca
     4441 tcgtgtttgg tgcattgatt ggcgcacttt ccagatattt ttggcatttt attgcaggag
     4501 tggttttctg gggagcgtat gcactatggg ggatgaatcc ttggctcttt tctttcgtaa
     4561 tgaatggtgc cagcgggttg agtacagcta ttgtaacgat catcgtttta ttagggatcc
     4621 aacaaacgac tccttcgatt tttgtgccag ataaacgaca ggagctgtaa ataaaaaaga
     4681 agcaagaaac ttgagacaga atcatgtcct agtttcttgt ttccttttta ttttttgata
     4741 agaaatagat ctacttcaat ggcagataag aaagatcggc atcttctacc aattgccagt
     4801 gtcgatcggt gacagagtag attaaagtag cgatgatttc ctcttctcga taccgggtgc
     4861 cttttaattt ccaaatataa tcttcaatca catagattgg gatacgatat ttaccatcga
     4921 tcaaaccaac ttgatcaaaa gaaaaaatga tcccttcctg catcaaatta cgcatttctt
     4981 tcaataattc cacactgatt ttttcaacag gtgtttcttt tttgaaaaaa gattttttta
     5041 cattgatctt atctgacaag atcgaactta atttactagc aaaaatccta taaaattcat
     5101 ctaaatagcg ataataaatc cgattcagct gatcattaag atcgaagtat acaaaattat
     5161 tctgtagttt ataaaaaaac ggcgaatgaa gatgtgtttt catatgacca aaatataata
     5221 attctgaaat ttctaaaggt gtcaattctt tcagcatcgt taaatcagta aagtcgatcc
     5281 acttcaattc atcgaatcca cgcctgttac gagaagagga aaagaaacgc tgaatatttt
     5341 ctgtaccacg gattactttc agccctgtat gggattcata ttctccagca gaagcagatg
     5401 gatcaagcaa gagcaagttt tgaggaaaat gcacgatact gtttgcaaag tcagtagcag
     5461 tcagcccttt tgttaaaaca gcattacttg tggtatcaat atgtacataa actgactcag
     5521 tcatcatgtt cctcctctta tttcctctta atctctttta ttttacaaca tttgcattaa
     5581 aaaagcttgt ttcttttatt ttaaaattcc attacaaaat cacgttttca ttgaaaacac
     5641 tctcattttc aattaatttt tgtgattttt gtattttttt atttaaagga ggttttatga
     5701 attgttttct tatggaatct acaaagtcga atacgagact catgaccttt ttatgacgta
     5761 ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta cttttcacag
     5821 aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt ttttcaaagt
     5881 catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc ttttaaagca
     5941 gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga tgttattttt
     6001 aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg gtatatggaa
     6061 gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc tcctctatca
     6121 gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca attaaataaa
     6181 aagctggtct atcatacagc acttgtttta ggtggaggag gagcgcacgg tgcttatcag
     6241 attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat cacaggtacc
     6301 tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa agctgtgaat
     6361 ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc tgcagttgaa
     6421 gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc actaattgaa
     6481 ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga tttgtcctta
     6541 ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc agaactagcg
     6601 gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg gatactcgcc
     6661 tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa atatgcagac
     6721 ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc aacagaggca
     6781 tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt
     6841 atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt tgatagccaa
     6901 aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt aggctattac
     6961 tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg gcgaggattt
     7021 ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag cataaaattt
     7081 tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg cggattggcg
     7141 atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta tcaagtagac
     7201 atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga tttattggca
     7261 caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata
     7321 caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa acgcaaggat
     7381 cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct atatttatat
     7441 tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta gaagaaatcg
     7501 ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc agctggaatg
     7561 gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa atggggaaag
     7621 gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa aatatgtaat
     7681 gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat ccgcgaaaca
     7741 aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct agatgttcaa
     7801 atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct tattaaacag
     7861 cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg caaaaacgaa
     7921 aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt gacagacgga
     7981 attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc
     8041 gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat ggaagaattg
     8101 gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc gagtcgagaa
     8161 aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt caaagaagta
     8221 ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa
     8281 aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt gacggagcta
     8341 gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc gatccatgta
     8401 caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg
     8461 atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc aatcgaaatt
     8521 aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca agtacaaaat
     8581 attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc cacgaattaa
     8641 gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt gacgtatgga
     8701 aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac gacggtgctt
     8761 acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta gaagatccga
     8821 cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact ttgtccaata
     8881 tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataatcagaa ttggatttct
     8941 tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa
     9001 tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt a
//
LOCUS       DACDYM010000088         8956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDYM010000088 DACDYM010000000
VERSION     DACDYM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.089"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..357)
                     /locus_tag="IWM59_002313"
     CDS             complement(<1..357)
                     /locus_tag="IWM59_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9980309.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
     gene            complement(709..1068)
                     /gene="rplT"
                     /locus_tag="IWM59_002314"
     CDS             complement(709..1068)
                     /gene="rplT"
                     /locus_tag="IWM59_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9980310.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="IWM59_002315"
     CDS             complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="IWM59_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9980311.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1393..1917)
                     /gene="infC"
                     /locus_tag="IWM59_002316"
     CDS             complement(1393..1917)
                     /gene="infC"
                     /locus_tag="IWM59_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9980312.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2237..2869
                     /locus_tag="IWM59_002317"
     CDS             2237..2869
                     /locus_tag="IWM59_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9980313.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2871..3776)
                     /locus_tag="IWM59_002318"
     CDS             complement(2871..3776)
                     /locus_tag="IWM59_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9980314.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3861..4514)
                     /locus_tag="IWM59_002319"
     CDS             complement(3861..4514)
                     /locus_tag="IWM59_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9980315.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            4836..5780
                     /gene="mvk"
                     /locus_tag="IWM59_002320"
     CDS             4836..5780
                     /gene="mvk"
                     /locus_tag="IWM59_002320"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9980316.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5773..6750
                     /gene="mvaD"
                     /locus_tag="IWM59_002321"
     CDS             5773..6750
                     /gene="mvaD"
                     /locus_tag="IWM59_002321"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9980317.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6763..7848
                     /locus_tag="IWM59_002322"
     CDS             6763..7848
                     /locus_tag="IWM59_002322"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9980318.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7845..8909
                     /locus_tag="IWM59_002323"
     CDS             7845..8909
                     /locus_tag="IWM59_002323"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9980319.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
BASE COUNT         2692 a         1859 c         1736 g         2669 t
ORIGIN      
        1 acctaatgac atcaagatcg gcaacacaat aactgcaata gaaatcactg gtccgattcc
       61 tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg
      121 cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac
      181 tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc
      241 agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc
      301 cgagtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat
      361 gacaaaagcc gaccatcaag ttgtagcctt ttacacagta ccagaccatg aaaaagaatg
      421 agataactaa caaaatacca ataataatat cactcatgtt attcctccgt ccattttgtt
      481 tttctgataa gcaaactaat atatcagtca aactcattct aacttatgta gttagcgctt
      541 tcattaaagg atgaccataa atgttcagtt ttcaattaaa aaaccttaat tatgttattt
      601 tagacttata cgttattgtc ctgtttcatt tgatggagga ttgtaaataa aaaaagagga
      661 aagaacataa cttttcgtta tcttctttcc tctttataat tgtttagctt atttagccaa
      721 agcttctttt gcttggtcag ccaatgcagt aaatgctgct gcatcgttaa cagccaagtc
      781 agctaacatt ttacggttga tgtcgatttc agcaactttc aaaccgtgca ttaattttga
      841 gtagctcaag ccattcatac gagctgctgc gttgatacga gcaatccaca atttacggaa
      901 gtcgcgtttc ttttgacgac gatcgcgata tgcgtagttg tatgaattca ttacttgttc
      961 ttttgctgtt ttaaataaag tgtgttttga accgtagtaa ccttttgcta attttagcac
     1021 tcttttacga cgtttacgag ttactgttcc acctttaaca cgtgccatgt ataattcctc
     1081 ctaataaaaa taaatcttca gattctgtta tttgtacttc ttatttcatt tttgctagtt
     1141 gttggcgaat acgtttgaag tcgccactag aaaccatgct tgctttacgc aattgacggc
     1201 gttgtttttt tgttttgccg tggaaacggt gacttgtaaa cgcgcggaag cgtttcaatc
     1261 cgcctttacc agtacgtttg aaacgttttg ctgatccgcg gtgtgttttt tgttttggca
     1321 tgactatatt tcctcctcaa aatctttcgt tatccgacta tcggctgtcc aacagccagt
     1381 tagtcattca aattacttac tgtcgttttt cggtgccagc gtcaagaaca tgctgcgtcc
     1441 gtccattttc gctttttgtt ccactgttgc aatatccgca gtttcttcag ctaagcgatt
     1501 caagactttt tgaccaattt ctttatgggt aatggcacgg cctttgaaac ggatagaagc
     1561 tttcacttta tcgccctttt ccaagaactt acgtgcatta cgaagttttg tattgaagtc
     1621 attcaagtcg atcgtaggac ttaagcgaac ttcttttaca ttgatcactt tttgtttttt
     1681 acgcgcttcg cggtctttct tttgttgttc gaaacggtat ttaccgtagt ccatgattcg
     1741 cgcaactggt ggtttcgcac caggggcaac taacacaaga tccaagtttg cttgttctgc
     1801 aatattcaat gcttctgctt ttgtcttaac tcctaattgc tcaccatctg aaccgatcaa
     1861 tcgtaactct cgtgcacgaa tgccgtcgtt aaccatcata tcctttgcta tggtcattca
     1921 cctccaaata ttatgagaga aagaaaagct taccgaataa acggcaataa aaaaacgagt
     1981 tctcttacag aacccgcgcg tacttcacct aatagaacca gtgaaaacta tctagcccag
     2041 tgacattatc aactaaggcg agaagcgggt gcttctgctt ttatttctca accattatag
     2101 tatagcaaaa actaatcttt ttgtcaatgt ttttcataca gtttgtcttc cttttctgaa
     2161 aatgattaga ttagcaggct ttctagcagt gattcccgct tctttgctat actaaagaaa
     2221 aacgaggtga acccacatga aaaaaatcga acctattcca ccttctcttc ctcttcttac
     2281 aaagggagag ttttatttag aagatgagga gatcttctct ggtcttctga tcaacggagg
     2341 taaccaaagc tatctcgatg ccaaaaatat ggtattaaga gagagtcata tccaaaaact
     2401 tacgatgcaa aaaacaggtt tacgtcattt tgaatgcagt aatgtcattt ttgaaaaatg
     2461 tgatttttcc aatctggagt ggattggtgg cagtttccac caagtcgtgt ttcatcaatg
     2521 taaattaaca ggtacgaatt ttgccgaaag ttatcttcga gactgtactt tcactgattg
     2581 tattgcttcg atggcttcct ttagtagtac aaacttgaaa gctgtctcct tcgatcattg
     2641 tcaattagaa gacagcgaat tttatgaggt cacttggaaa aacctttttt tgtctgacaa
     2701 tcagttaacc ggttcgaact ggtttcgaac ttctctgaag ggattagact ttacgactaa
     2761 tttcttcaca acaatcgctt tatctcaaga ttctttggcg ggactaatcg tcaatcaaga
     2821 gcaagcactt gtgatcgccc aagcccttgg tttgcagatc aaagactaga tcaacgaatc
     2881 cgccgccaca gtgcaataag catccaaata gataagacca aactgatgat caaggcgatt
     2941 tcccatccga aaacggaatg ggtaaagggt aactggagat tcattccgaa gaatcctgtc
     3001 acaatagttg gaacggtcaa gatcagcgac cagaccgtca aaaatttcat cgtgtcattc
     3061 aagttgttat tcagcaaatt attataagtg ccagagagct ggtctaaaat ctgcgaagct
     3121 aagttcgtca tttcaactgc ttgtcgtgct tcgatcagtg catcgtccaa ctcttctttt
     3181 tccttttctg acaacttgcg ataaatagcc agtgccttga tttgttctaa aagtactaca
     3241 ttttgttttg ttcctgtaac taagtaaacc aaaccgatct ctaaatctga aagttgcagc
     3301 agattcttgt tcgttgtttt ttctcttaat ttcagattaa gccgctgacg ttcactattc
     3361 acttcctcga ttaaaggaaa aaaggaatca gaaatcaaaa acaatgtacg aaacaataaa
     3421 ctgtatacac tcatgtctgg catccgttcc aaaagcgatc gaattagtcg aatcacgtac
     3481 tcggtattcg gcgtggtaaa tgtaaacaaa ccgtcttctt ttaaaataaa agcgatagga
     3541 ctagtttcat aatgattatc gactttttgc tggtgcggaa catttgatac aagcagtaac
     3601 gcttcatcaa aagaatcgta ctctacgcgc gcccgttcat ttttatctag agaataagtc
     3661 agcatctcgt cactaatgcc atattcttct tttaagtttt taacagctag cacatcgttt
     3721 tgctgtacat tgatccaaaa attacggtct tgattaaaat agtgtttttc ccgcatactc
     3781 tatcctcctg acatttgttt ccagtataaa gaaaaaagtc gctcaaataa aggtttaccc
     3841 tttatttgag caactgtaaa tcaaatggat actttggttt ggcgaatctc tgctaatttt
     3901 tcttcgatca ttttcaagac ttcttctttt gcttgaggat cttccacaaa attatagcgg
     3961 tctccatcga tttggatttt tggactttct ttgtattctt cataccattg atcataacgt
     4021 ctgttcaatt ctttgtaata atcgtataga cttgggtcaa aggacaattg ttcatacgaa
     4081 cgaccacgtt tttcgatacg ctctaacatc gtatcaaaag atacacgaat atgaactaat
     4141 aaatccggat gttttttatg ggcagcatag ggaagttctt ccatcatgtt ttccagcagt
     4201 tcatcataca cacaaacttc tgtttctgtt gctcttccta gatctgcatt tagatggaaa
     4261 agcagtgaat cttcataaat cgaacggtcc aatacattat tgtcatcttt cattgcttgt
     4321 ttgatgcttg caaaacgttt attcaaaaaa taaatttgca gcaaaaaagc atactgttca
     4381 gggtttgcat aaaataaggg caagacttca ttatcatcaa tcgattcata aaatgcctga
     4441 ctatcaaagt gttcagccat catttctgtt aaacttgatt ttccggcacc aattgtacct
     4501 gctaaaacga tcacgctcat ttacctccag ctcacttcat cactaaatat tgtgtttaac
     4561 tccaaccttt tttaaatata cccccaatat ctagtagtgt caattgtatt ttttctagtc
     4621 aaagaagaaa aaaagtcaaa aaattcttaa gaaaaaagtg ttttttcttt agaaaacgta
     4681 ttcttagttt tacttctgag atggttttag taaaataaag gaggaatgtt ttttgagacc
     4741 cttagtggac aaaaaaattc gtgaagcagc gcaactcgtt ttgggcgctt tattttttgc
     4801 ttaaaaatca cttttacgtg agaaggagtt agattatggc aaactatggc caaggagaat
     4861 caagcggaaa gatcatattg atgggcgagc acgcggttgt ttatggagaa ccagcgattg
     4921 cctttccttt ctatgcaaca aaagtcaccg cattccttga agagctggat gcaatggacg
     4981 atcaactggt ttcttcctac tattcaggaa atttagccga agctcctcat gcattaaaaa
     5041 atatcaaaaa attattcatt cacttaaaaa aacagcatga catccaaaaa aacttgcaac
     5101 tgaccattga aagcacgatt cctgctgaac gtggaatggg atcaagcgct gcagtcgcca
     5161 cagcagtcac tcgtgctttt tatgattact tagcatttcc tttgtctcgt gaaatactat
     5221 tagaaaatgt ccagctttcg gaaaaaatcg cccacggtaa tcctagtgga atcgatgcag
     5281 ccgctactag cagcttgcag ccgatttatt ttacaaaagg gcatcctttt gactactttt
     5341 ctttgaacat cgatgctttt ttgattgtcg ctgatacagg aatcaaagga caaacaagag
     5401 aagccgtcaa agatgtcgct cacctctttg aaacccagcc tcatgaaact ggacaaatga
     5461 tccaaaaatt aggatacttg acgaagcaag caaaacaagc aatcattgag aattcaccag
     5521 aaacgttggc acagacaatg gatgaatcac agtcacttct ggaaaagctg acaatcagca
     5581 atgattttct taatctattg atccaaacag caaaagatac cggggcattg ggtgccaaat
     5641 taactggcgg tggacgcggt ggctgtatga ttgcattagc acaaacaaaa acaaaagccc
     5701 aagaaatcag tcaagcactt gaagatgcgg gtgctgctga aacttggata caaggattag
     5761 gagtacatac ctatgtttaa aggcaaagca cgcgcatata ccaacattgc tctaatcaaa
     5821 tattggggta agaaaaatga agaactcatc cttccaatga acaacagcct ttcgttaaca
     5881 ttggatgcat tttatacaga gacagaagtc atcttttctg atagctatat ggtagatgaa
     5941 ttttatctag atggcacctt acaagacgaa aaagcaacaa aaaaagtcag tcagtttctt
     6001 gacctttttc gtaaagaagc cggcctctca ctaaaagctt cagtaatcag tcagaatttc
     6061 gttcctaccg cagctggatt agcctcctct gccagcgggc tagctgcttt agcaggagct
     6121 tgcaatactg ctcttaagct tggattagac gatctctctc tttcaagatt tgctcgacgc
     6181 gggtctggtt cagcttgccg aagtattttc ggtggtttcg ttgaatggga aaaaggccat
     6241 gacgacttaa gttcttacgc taagccagtc ccttccgatt ctttcgaaga cgatttagca
     6301 atggttttcg ttttgatcaa cgaccagaaa aaagaagtgt ccagcagaaa tgggatgcgt
     6361 cgaacagtcg aaacatccag tttttatcaa ggctggttag attccgttga aggggatcta
     6421 tatcaattga aacaagcaat caaaacaaaa gatttccaac ttctcggaga aacgatggaa
     6481 agaaacggac taaaaatgca tggaacgact ttagctgccc agcctccatt cacttactgg
     6541 tctcctgaca gcttaaaagc gatggatgcg gtgcggcaat tgagaaaaaa aggcattcca
     6601 tgctacttta cgatggatgc tggccctaat gtcaaagttt tagtggaaaa cagccattta
     6661 tctgaggtac aagaaacatt taccaaatta tttagcaaag aacaagtaat tactgcccat
     6721 gctggtccag gaattgcgat tattgaataa agggagattt ttatgattga agtatctgca
     6781 ccaggcaaac tctatattgc cggagaatat gcagttgttg aaacaggaca tccagcagtt
     6841 atcgctgcag tcgatcaatt cgtaacagtc actgtagaat ccgcacgaaa agtcggaagt
     6901 atccaatctg cacaatatag tgggatgcct gtacgttgga caagacgcaa cggagaatta
     6961 gttttagata ttcgggaaaa tccttttcat tatatccttg ctgctattcg tttgactgaa
     7021 aagtatgcac aagaaaaaaa cattctttta tccttttatg atctgaaagt aacaagtgag
     7081 ttagacagtt caaatggccg gaaatatgga ttgggttcta gcggtgccgt gaccgttgca
     7141 acggtcaaag ccttgaatgt tttttacgcg ttgaatttat ctcaattgga gattttcaag
     7201 attgcagcac tagccaattt agcagttcaa gataatggtt cctgcggcga catcgctgct
     7261 agctgttatg gtggctggat cgctttctca accttcgacc atccttggct ccaagaacaa
     7321 gaaactcagc attctatcag tgagttactt gccctggatt ggccaggtct atccattgag
     7381 ccattgattg ctcctgaaga tttacgttta ttgattggtt ggacgggtag ccctgcctct
     7441 acttctgatt tggtcgatca agttcaccgt tcgagagaag acaaaatggt ggcttatcag
     7501 cttttcttaa aaaacagtac agaatgtgtc aacgaaatga tcaaagggtt taaagaaaat
     7561 aatgtaacgt tgatccaaca gatgattcga aaaaaccgac aattactgca tgatttatct
     7621 gcaatcactg gggtcgtcat cgaaacgcct gctttgaaca aattgtgtaa tttagctgaa
     7681 cagtatgaag gagccgcaaa atcttctggt gcaggtggtg gcgattgcgg aatcgtaatt
     7741 gttgaccaga aatctggcat ccttccttta atgagtgcat gggaaaaagc agaaatcact
     7801 ccactgccgt tacatgtcta tagcgatcaa agaaaggaaa accgatgaat cgaaaagacg
     7861 aacatgtttc actagctaaa gcctttcatg ataaacaaaa aaacgaattt gacttcgtac
     7921 ggatcatcca taaccctttg ccgcaaatag cagtttctga tgtcgatcta agtacacagg
     7981 cagttgggtt tacactaagc agcccatttt atatcaatgc gatgactggc ggaagcgaaa
     8041 aaacaaaaaa aatcaaccaa gatcttgcaa tcgtcgcaag agaagcagat ttgatgattg
     8101 ccactggttc agtcagcgct gccttgaaag acccttcttt agccgatact tatacgatta
     8161 tgcgacaaga atatcctcac gggaaaatca ttgccaacat cggtgcaggg acttctgtcg
     8221 aaagagccca agaagctatc cgattgtttc acgcagatgc cttacagatt catttgaatg
     8281 cacctcaaga gttggttatg cctgagggag accgtgattt taccaattgg aaagtactta
     8341 ttcaagagac tcaaacagcc attgacgtcc ccctcatcgt aaaagaagtc ggttttggca
     8401 tgacaagaga aacgctcaat gatctagctg ctttaggagt tcatacagtt gatatcagcg
     8461 gtcgaagcgg aacaagcttc acccagatcg aaaacgcccg tcgctccaag cgggaactga
     8521 gttatttagc tgactggggg caatcgacag tctcttcatt gcttgaagca aatgaagcag
     8581 acacttctat ggaaatccta gcctccggag gtatccgcaa tgcctatgac atatttaaag
     8641 ccctttgtct cggcgcaaat gcagtgggta cttcaggaac cgtactgacg catctgatga
     8701 atcacggcgt agaagaaact attatactga tgaagcaatg gcaagaagag cttcgtctgc
     8761 tttatactat ggtcggcgca acgaataccg cggccctaca ccaacaatca ctgatctttt
     8821 ccggaccagt gaaggattgg tgcgaagcaa gagggatcga cttagtgaag tatggaagac
     8881 gcacagaaaa gagcgtggga caaaagtaaa gaacactttt atcccacgct ctaaacacgt
     8941 ataaacggcg gaagca
//
LOCUS       DACDYM010000089         8942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDYM010000089 DACDYM010000000
VERSION     DACDYM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.090"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..260
                     /locus_tag="IWM59_002324"
     CDS             51..260
                     /locus_tag="IWM59_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980320.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IWM59_002325"
                     /pseudo
     CDS             467..667
                     /locus_tag="IWM59_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="IWM59_002326"
     CDS             complement(1379..1984)
                     /locus_tag="IWM59_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9980321.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IWM59_002327"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IWM59_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IWM59_002328"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IWM59_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IWM59_002329"
     CDS             3019..3990
                     /locus_tag="IWM59_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9980322.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IWM59_002330"
     CDS             4130..5443
                     /locus_tag="IWM59_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9980323.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IWM59_002331"
     CDS             5486..6352
                     /locus_tag="IWM59_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9980324.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IWM59_002332"
     CDS             6349..7197
                     /locus_tag="IWM59_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9980325.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IWM59_002333"
     CDS             7211..8683
                     /locus_tag="IWM59_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9980326.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="IWM59_002334"
     CDS             8700..>8942
                     /locus_tag="IWM59_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9980327.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DACDYM010000090         8804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDYM010000090 DACDYM010000000
VERSION     DACDYM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.091"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..239
                     /locus_tag="IWM59_002335"
     CDS             <1..239
                     /locus_tag="IWM59_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP9980328.1"
                     /translation="LYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHS
                     LAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            306..800
                     /locus_tag="IWM59_002336"
     CDS             306..800
                     /locus_tag="IWM59_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9980329.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            856..1494
                     /locus_tag="IWM59_002337"
     CDS             856..1494
                     /locus_tag="IWM59_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980330.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1566..1961
                     /locus_tag="IWM59_002338"
     CDS             1566..1961
                     /locus_tag="IWM59_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9980331.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            1945..4398
                     /locus_tag="IWM59_002339"
     CDS             1945..4398
                     /locus_tag="IWM59_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9980332.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4395..6422
                     /locus_tag="IWM59_002340"
     CDS             4395..6422
                     /locus_tag="IWM59_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980333.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6419..7441
                     /locus_tag="IWM59_002341"
     CDS             6419..7441
                     /locus_tag="IWM59_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9980334.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7458..8387
                     /locus_tag="IWM59_002342"
     CDS             7458..8387
                     /locus_tag="IWM59_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9980335.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            8632..8748
                     /locus_tag="IWM59_002343"
     CDS             8632..8748
                     /locus_tag="IWM59_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9980336.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2978 a         1479 c         1759 g         2588 t
ORIGIN      
        1 aattatatag taggatacaa caattaattg aaaaagaaat tcaacctact gaacttgctg
       61 atatagtgaa gacaacgata aggtcatcaa atggaatgat tagctctctt aatgaagcaa
      121 gaaataggca ttcacttgca caccctaata caaatattat tggaaagaga gaagcaaaac
      181 taataatagg tatttcaagt acagttacag attatattag cggatacctt gataagtagc
      241 gtcattaact gattgctact ttttttatga ctatttttat aaaaaggatg tgataagaat
      301 tggaaatgaa agtctatgtg gcaaatttag gacggtataa tgaaggtgaa ttagtcggag
      361 catggtttac tccaccaatt gatgaggaag aaatggcaga aagaatcggt ttaaatgagg
      421 actacgaaga atatgcaata catgattttg agcttccttt tgatgtggac gaatacacgc
      481 cgattagtga aatcaaccgt ctatgtgaag ccattcaaga aatcgaaggt acacctattt
      541 ataatgaact caaagaaatt caaggcatgt ggtttagtag cttggaagaa ttactggaaa
      601 acaaagagga tattcattgt tatagtgatt gtgattcgat ggaagatgtc gcccgttatt
      661 acgtggaaga aacaggacag cttggtgaag tcccttccaa tttacaaaat tatattgact
      721 accaatcttt gggacgtgat atggaaatag agggtaatta cctagttacc tctcatgggg
      781 tatttgaata ttgtcagtaa gtttaacaat ccgtgttata attaaattaa taaatagcac
      841 ggagaggtat aaattatggt aaatgtattt gaaaatctta aactattaaa actagacatg
      901 aaagataaag gctgggtgat agattccttt tactttcgtt acaaacagca aaattatatt
      961 gtacttgtta agctatttga aaaagaggaa aaagttccag aatatgctct gttaaaacta
     1021 gaatttttaa aggaaaacga tttttcagat atgctagctg tctatgctaa ttcggtaaaa
     1081 ttatatacag acacgaaaac aataagagaa tattttggta tcgaatacag tagtaacttg
     1141 ggagatgttt tatttcaatt tagccaaact cttgctcgat ttattccgac agaagttagt
     1201 gagaagaaaa atgaagacca aaaagaagcc atgtgtttct cattaagtca aagtgattca
     1261 gaagatccca gaaaaaaata ttgtttttca gttagaagaa atcctttaaa gggaaatggt
     1321 gagctgggaa aacgaagtcc ttttaatgac aataaaacga gattgtatag acccagctta
     1381 tatgaacgat tagggtcaga taccaattta agtttcagat attcaatgaa tccagatgat
     1441 gaagaaactg atgaaggtat tattgcaaaa tggacaaaaa ataaaattga atagttaaag
     1501 cattgaaaga gcaatcacct attttgattg ttctttttct atttttacgg aaaggacgtg
     1561 aagacatgaa aaaaataaga agttacacca gtatttggtc agtagaaaaa gtgatttatg
     1621 cgattaatga tttacaactc cctttcccaa ttacctttac gcaaatgaca tggtttatcg
     1681 tttctttgtt tgtcgtcata ctctttggca gtttaccacc actttctctg attgacggag
     1741 cactactcaa atacttaggg attcccattg ttctcactgg gttcatgtgc caaaagacct
     1801 ttgacggaaa gaaaccctat ggatttctaa agtctatcat tacctatgct ctacgcccta
     1861 aagtaaccta cgctggcaaa caaatacgat taaaaaaaga aacaatgaat gaatcgatta
     1921 cgattgtaag gagtgaacaa ctagatggca tatccgatta aatatatgga agataatctc
     1981 gtatttaatc atgatggcga agtattcgct tattatgaat taattcccta taattacagt
     2041 tttttaagtt ccgatgaaaa aattcaagtt catgataatt tctgtcaatt gattgcccaa
     2101 aatcgtgatg gcaagattca tgctctgcaa ctagcaaccg aatcaagtat tcgagccaca
     2161 caggaacaat cgaaaaaaga aatcactgga cgattaaaag agatagctta cgaaaaaata
     2221 gatgaacaga ccgaagcgtt aatttctatg attggtgaca atcaagtaga ctatcgcttt
     2281 tttattggtt ttaaactcta cctcaatgaa caggaagtat ccgtaaaaaa tgttgggaaa
     2341 gaaattcaaa aagcatttgc ggaattcttt catggtgtga atcacaaatt aatgggcgat
     2401 tttgtttcta tgcctatggc ggaaatcgaa cgcttttcta agatggaaca gttgttagaa
     2461 agtaaattat ccagacgctt tcagattcgt aaattggaga aagatgattt tggttatatt
     2521 attgaacatc tttacgggca aacaggtgtc ccttttgatg actacgacta ccatttaccg
     2581 aaacgatacg aagataaaaa agtgttggta aagaaatacg atattctcaa acccactcgc
     2641 tgtttaatcg aagaaaatca acgctattta cggattgaac aggaagatac cactacttat
     2701 gtggcgtatt ttacaattaa tagtattgtt ggtgaattgg atttccctaa tagtgaaatt
     2761 ttttattacc agcaacagca gtttactttc cccgttgata ccagtatgaa tgttgaaatt
     2821 gtcgccaata aaaaagcctt atctaccgtt cgcaataaaa agaaagaact gaaagattta
     2881 gatagccacg catgggaaag cgacaacgaa accagtacta atgtggtgga cgctttagaa
     2941 tctgtcgatg aattagaaag taatttagac caaaccaaag aatctatgta caaactaagc
     3001 tatgtggtac gggtatcagc aaagaatttg gacgagctga aacaacgttg tgatgaagta
     3061 aaagattttt atgatgatct gtctatcaaa ttagtacgac cttttggcga tatgctaggc
     3121 ttgcataatg aatttattcc ctcaagcaag cgatatatca atgactatat tcaatatgtc
     3181 acgtcggatt ttttggctgg tcttggtttc ggtgctacac aaatgattgg tgaaaattct
     3241 ggtatttacg tcggttacaa tctcgataca ggcagaaata tttatctcca acctagcctt
     3301 gcaagtcaag gaattaaagg ctctgtaaca aatgcattag cgtccgcttt tattggttca
     3361 ttaggtggcg gaaaatcatt ctctaacaat atgctggtct attattcagt actctttggc
     3421 ggacaggcaa ccattgttga cccaaaagct gaacgtggtg aatggaaaga aaccttacct
     3481 gatattgccc atgaaatcaa tattgttaat ctcacgagtg agaacgacaa taaagggtta
     3541 cttgaccctt atgtgatttt aaaactcccg aaagattcag aaagccttgc cattgatatt
     3601 ctcacgttct taacaggtat ttccagccga gatagcgaga agtttcctgt cctgcgtaaa
     3661 gctattcgga gtgtaactca aagcaaacaa cgagggttgt tattggtgat tgatgaattg
     3721 cgaaaagaag atacgcccat cagcaatagt attgctgacc atatcgaaag ctttgtcgat
     3781 tatgattttg cccatctgtt attcagtgat ggcagtgtca cccaatccat tagcttggaa
     3841 aaacaactca atattattca agtggcagat ttggtcttac ccgattccaa tagctctttt
     3901 gaagaataca ctaccatgga attattaagt gtggcaatgc tgattgtcat tagtaccttt
     3961 gctttagact ttatccactc tgaccgttcc atttttaaaa ttgttgacct tgatgaggct
     4021 tggagttttt tacaagtggc acaaggaaaa gctctatcta tgcgattggt tcgagcagga
     4081 cgtgccatga atgcaggggt ctatttcgtt actcaaaacg cggacgattt actagatgaa
     4141 aagatgaaga ataatattgg cttgaaattt gcctttcgtt ccactgacct agtggaaatc
     4201 aaaaagacct tagaattttt tggtgtggac aaggaagatg aaaacaatca gaaacgctta
     4261 cgtgaattag aaaatggaca atgtttagtt tcagacatct atggtcgtgt aggggttatg
     4321 cagtttcacc cgatttttga agagttgctt catgcctttg ataccagacc gcctgtgcga
     4381 aatgaggtgg aagaatgaca caaaaacaaa agaaatttct tcactatagt tggataacgc
     4441 tcctttgtgt gggcgttatt ttacttttat taggcacgct gggcaatgca gtacaagcga
     4501 caggcttagt tgatgaaacc attgatacaa gcaatgaata ctccaagtat ggtttgaatc
     4561 aatatcagct tgattattat gtggacaata gctggggctg gcttccatgg aattggagtg
     4621 atggcatagg acaatcggtg atgtatggat tgtatgccat tactaatttt atttggacaa
     4681 tttctcttta tctatcgaac gccacaggtt atttggtgaa agaagcctat gccttagatt
     4741 tcattagtca aacatcccaa gcaattggcg aaaacatgca gacgattgca ggcattacac
     4801 cgaatggctt ttccagttca gggttttatg tcggtttcct ccttcttttt attctcattg
     4861 ttggaatcta tgttgcctat acaggattgg ttaagcgaga aacgacgaaa gtgatacgag
     4921 ctgttttaaa ctttgtcgtg gtgtttattc tatcggcttc ctttattgcg tatgcacccg
     4981 attatattgc gaaaatcaat gatttttcca aagatgtcag taccgccagt ctgaatattg
     5041 ggacaaaaat tgttcttcct aacacggata gtcaagggaa agacagtgtg gacatgatac
     5101 gtgacagctt attctctgtt caagtaaagc aaccatggct attgcttcaa tatggctcca
     5161 ctgatgtaga agcgttaggg gaagaacgtg tggaaagttt attgtctacc agtccagata
     5221 caaacaatgg tgaagataga gaaaatgtag tgattgaaga aattgaagat catgacaata
     5281 cctatttaac cctaccgaag acgatttcca gattaggaac agtcttcttt ttgtttgtat
     5341 tcaatattgg tatctcgatt tttgttttcc ttctaacagg aatcatgatt ttttcgcaag
     5401 tgctatttat tattttcgct atgtttttgc caatcagttt cctattaagt atgttgccga
     5461 gttttgatgg catgggcagg catgccatta ccaagttatt taatgtgatt atgacaaggg
     5521 ctggtattac cttgattatt accgttgcct ttagtatttc cactatgctt tattcgctgt
     5581 cagcaagttc tccctttttc atgattatgt ttttacaaat tgtgaccttt gcagggattt
     5641 attttaaact gggcgattta atgagcctat tcactttaca aagcaacgac tctcaaaaca
     5701 tgggcagaca agtgtttcgc agacctcgtc aaatgatgaa tcgacaaacc agaagatttc
     5761 aacgcagtat gagtaagatt tttcgtggaa aagcagggtc aacgaagcaa aagacaatga
     5821 caacaaagca gaatcaaacc aataaagcta accatactcg tcaaaatgaa cggactacac
     5881 caaagaaaga aacagcactt tccaatgtga aaaagcaaca aggcggtatc gggcaacgat
     5941 taggtgaaaa gacagcccat gtattggaca cgaaaaatcg tctagtcaat aaaagaaagc
     6001 aggcaaaaca acagatacag gcgacaccaa cccatgtcaa atatgccctt catcaaggaa
     6061 aagagaatgt cactcataat gtaacagact ttaaacaatc tatggcagat accaaagctc
     6121 aacgcaaaca acagcgtatg aaccagcagg agcaaaaacg aaagaacatt gctcaaaagc
     6181 gtttggagct ggataaagca aaagaaaaaa gacgaaaaga aaacccatca tctcctgttc
     6241 agaataacca gcagcatgtt cgcaagcgtc agcaaaccgt cacgcctgtt ttgtcacaaa
     6301 acactaaacg tgaaaatcat cagcgaccat tgaaaaaaga gcaaaaacaa aagacaccac
     6361 cgaagcaatt caatcagagg aagctaaata aaagaacgga taagcgagga caacaaacat
     6421 gaagctaaaa ataacagtga tttgtagtgt ggtcttctct ctcttctttt ttctcttatt
     6481 attcctagtc attttctttg cagatgatac ggacagtggc gaaaacaaca aggattcttc
     6541 tattccacaa ggcggtgtga ccgtttcacc agaagtgttg gcacaccgtc cgttgattga
     6601 aaaatatggt aaggaatatg ggattgaaga ctatgtttct tatatcttag ccattatgca
     6661 agtggaatcg ggcggtacag cagaagatgt tatgcaaagt tccgaatctt tgggtttacc
     6721 gcccaacagt ttgagtaccg aagagtccat caaacaaggg gttaaatatt tcagtgaatt
     6781 attaaccagt gcagaacaac aaggagtgga tatagattct gttatccaat cttataatta
     6841 tggcggtggc tttttaaatt atgtgaggag tcatgggaaa aaatatacct atgaattagc
     6901 cgaacagttt tctaaagaga agtcgggtgg tcaaaaagca gactatccca atcctatagc
     6961 catacctgtg aatggtggtt ggcgatataa ctatggaaat cagttttatg ttcagttggt
     7021 ttcacaatac ctaaccgaca cttccccaac agaatttgat gatgaaaccg tgcaagtcat
     7081 tatggacgaa gccttgaaat acgaagggtt tccctatgtc tttggagggg cgtcgcctac
     7141 cacctctttt gattgtagtg gcttaataca atgggtgtat gacaaagctg gcatttctct
     7201 ccctcgtgtc gcccaagatc agtatgacgc tacgcaagaa atctctatgg aagaagccca
     7261 agcaggcgac cttatcttct ttcattcaac ctataatgca ggcacatatg tgactcatgt
     7321 ggcgatatat ttagagggaa atcgctttta tcatgcaggc gacccaatcg gttatggcga
     7381 cttaagcagt cgttattggc aagaccatct gattggggct agacgtgtta ttcataacta
     7441 agaaaggaat gaatcaaatg attataaaaa tcgagcgaaa agaaaagaag gagaaaccaa
     7501 agaaatctaa aaaaccgagc aagcaaagaa aagttccgat gattaaagtt ggcacgcata
     7561 agaaattaac ctttgtttta tgggttttat tgattggtag tgtcggattt ggaatctata
     7621 aaaattttac tgccattgac actcatactg tccgtgaaac agaaatcatt aaacagcaga
     7681 ttgtcgatac caaccaagta gaaagttttg tgaaatcctt tgcgaaagac tacttttcgt
     7741 ggcaacaatc ccaagaagcc attgataaac gaaatgaaaa attgacccat tatttgacgg
     7801 aagaattgca ggtattaaat gaagaaatga ttcgtaagga tattcctact agttctagtg
     7861 tgaacgatat acaggtgtgg caggtttctc aagtgaatga gaacaccttt gaggtactgt
     7921 tttctgttga acaagttatt accgaagaca aagacaagga aactatatcg tctagctttc
     7981 atgtggttgt ccatatagat gaatcagata atatggttat tatcaaaaat ccgacaatga
     8041 gtaagaaacc tcaaaaatcg gactaccaac ccaaacagct agaaagtgac catacagtag
     8101 atacagaaac gatggacgaa atcactagct ttttagaaac gttcttccag ctctatccta
     8161 ccgccacaga aaaagaattg acttactatg taagtaatca tgtgttacct atgattaaca
     8221 aggaatatgt tttcgaggaa ttggtgaatc ctatctttac cagaaaagat aaccaagtaa
     8281 tcgtgaatgt cgctgtgaaa tatttagatc aagaaacaaa ggcaacccaa atctcgcaat
     8341 ttgagttgat attagaaaaa caagataatt ggaagattgt aaaataacaa atattggtac
     8401 atgattacag atactttgta atcatgtact ctttttgata aaaaattgga gattccttta
     8461 caaatatgct cttacgtgct attatttaag tgactattta aaaggagtta ataaatatgc
     8521 ggcaaggtat tcttaaataa actgtcaatt tgatagcggg aacaaataat tagatgtcct
     8581 tttttaggag ggcttagttt tttgtaccca gtttaagaat acctttatca tgtgattcta
     8641 aagtatccag agaatatctg tatgctttgt atacctatgg ttatgcataa aaatcccagt
     8701 gataaaagta tttatcactg ggatttttat gcccttttgg gtttttgaat ggaggaaaat
     8761 cacatgaaaa ttattaatat tggagtttta gctcatgttg atgc
//
LOCUS       DACDYM010000091         8382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDYM010000091 DACDYM010000000
VERSION     DACDYM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.092"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(51..1517)
                     /locus_tag="IWM59_002344"
     CDS             complement(51..1517)
                     /locus_tag="IWM59_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9980337.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IWM59_002345"
     CDS             1904..2500
                     /locus_tag="IWM59_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9980338.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IWM59_002346"
     CDS             complement(2558..3040)
                     /locus_tag="IWM59_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980339.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IWM59_002347"
     CDS             complement(3146..3343)
                     /locus_tag="IWM59_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980340.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="IWM59_002348"
     CDS             3638..4396
                     /locus_tag="IWM59_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9980341.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="IWM59_002349"
     CDS             4456..4884
                     /locus_tag="IWM59_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980342.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="IWM59_002350"
     tRNA            4930..5002
                     /locus_tag="IWM59_002350"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="IWM59_002351"
     CDS             complement(5152..5523)
                     /locus_tag="IWM59_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9980343.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="IWM59_002352"
     CDS             5738..5923
                     /locus_tag="IWM59_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980344.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="IWM59_002353"
     CDS             complement(6006..6194)
                     /locus_tag="IWM59_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980345.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="IWM59_002354"
     CDS             complement(6218..6481)
                     /locus_tag="IWM59_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980346.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="IWM59_002355"
     CDS             6639..6896
                     /locus_tag="IWM59_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9980347.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="IWM59_002356"
     CDS             complement(6967..7158)
                     /locus_tag="IWM59_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980348.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="IWM59_002357"
     CDS             complement(7299..7619)
                     /locus_tag="IWM59_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980349.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="IWM59_002358"
     CDS             complement(7642..8076)
                     /locus_tag="IWM59_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9980350.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2661 a         1650 c         1325 g         2746 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat
     8341 tttcacgtgt taaagtattc ctcgttgaac acaacaaata aa
//
LOCUS       DACDYM010000092         8292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDYM010000092 DACDYM010000000
VERSION     DACDYM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.093"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            434..601
                     /locus_tag="IWM59_002359"
     CDS             434..601
                     /locus_tag="IWM59_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9980351.1"
                     /translation="MVYLNKKQTCEFLGIDNNTLDKWIRIYNFPVIKIDGIIRFDVED
                     IKGFMDTFKKI"
     gene            675..1901
                     /locus_tag="IWM59_002360"
     CDS             675..1901
                     /locus_tag="IWM59_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9980352.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(2059..2676)
                     /locus_tag="IWM59_002361"
     CDS             complement(2059..2676)
                     /locus_tag="IWM59_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9980353.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            complement(3359..4354)
                     /locus_tag="IWM59_002362"
     CDS             complement(3359..4354)
                     /locus_tag="IWM59_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9980354.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
     gene            complement(4449..5321)
                     /locus_tag="IWM59_002363"
     CDS             complement(4449..5321)
                     /locus_tag="IWM59_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9980355.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            complement(5334..6761)
                     /locus_tag="IWM59_002364"
     CDS             complement(5334..6761)
                     /locus_tag="IWM59_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9980356.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            complement(6815..8089)
                     /locus_tag="IWM59_002365"
     CDS             complement(6815..8089)
                     /locus_tag="IWM59_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9980357.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
BASE COUNT         2746 a         1524 c         1185 g         2837 t
ORIGIN      
        1 tattttaaat ttttacatac ctgtttatga aaaactaaat aatctgtcaa aaaaatatga
       61 ttgttcattt ctaaatatgt tttccaattt taaaatcttt tttagcattt ttgtttcctt
      121 ttcatatggt ttttcgttgt taatagtgac taggatgata ttcctatttt gaccattgac
      181 aattaaatat aatttttgaa taacgttccc tgctattcga gctaagaatt caagtacgcc
      241 atgacctatt tagcgataac tacttagaga tttctttttg ttttgggaag acaaaatgcc
      301 atgacaatag cagtagataa tgatacttcc gtaaggctcg ccgtaaactg gggagaggtg
      361 aaattcctat gtgcttaccc aagtcaattc aaaaatttta gttcatttat aaggggatga
      421 ttttattaaa tcaatagtct atctaaacaa aaaacaaacc tgtgagtttt taggaattga
      481 taacaatact ttagataaat ggattcgtat atataatttt cccgtaataa aaatagacgg
      541 tataattcgc tttgatgtag aagatatcaa aggctttatg gatacgttca aaaagatttg
      601 aattttactt ttgactgcat atactaaaag tagcgaaata tactgcctta cttttttatt
      661 aaggagtgag gaagttgcct acgaagcttt caaatggaaa atataaaact aatttgagat
      721 atccaaaaaa atttaaagaa attacaggta tcagttcaga aaaattccaa aaaacatttc
      781 caactaggca attggctatt aaagccgaaa atgaaatgaa aaagaaaatc gcaaaagtct
      841 taagagagga gaatgcaaat tcattagagc tcaaaggtca aataaaattc aaggagtttt
      901 ataaatctaa atggcttcct agatatgagt tagggcaaac tactagatcc cataaagctc
      961 caagcgatat aaccatcatt aatacaaaga atttatttcg attgcatatt ctgcctatat
     1021 ttggtgagta tgccatgagc tatcttaatg caaataccga aataatatca gatgaactca
     1081 caaaaaagtc gaaggaatat gcaaacataa aaatcattaa atgctatgtt cgttccatgt
     1141 ttgatattgc agaggttcta aattatattg aatttaatcg tacaacaaaa ataattcaaa
     1201 gtattactgc acctaagaaa gttgctctgg aagaaaaaag aatacgagaa ggaaaacaag
     1261 ctttatcttc gaaagaactc actgattggt tagacgctgt aagagatgat ttcaatgatc
     1321 aattactttc ctttcacgat tacactcttt ttatgcttac tttatatctt ggggatagaa
     1381 agagtgagac ctatgctttg caatggaaat acattgattt tgaaaaccaa acagtccgtc
     1441 ttaaatatgc cttggataaa cacacaaaaa agaaatttac aaaaggtcga aaagatacta
     1501 ttatccaagt tccggaaatt gtaatgacac tattgcaaga atggaaatca gtgcaagctg
     1561 aacaactatt gaagttgaaa attaaacaga cacctgacca atatcttttt agttactcca
     1621 aaccttctgg tgaagtaaat tgtccagtcc atactgatta cttaaattat cgaattaatt
     1681 ctataaaaaa aagacatccc catttggttc atttaactcc tcataaactt cgacatactt
     1741 acgcaacttt agctagacaa ggtggggctg atatgaatca aatttctaac gctttgacac
     1801 attcaggtat ttcaactaca aaaatttatg tgaatacacc tgatttagtt gataaagctg
     1861 tgttcgaagc gtttcagcga ggattacaaa aatctaatta atgtgttcta gaatgtgttc
     1921 taaatataaa aaaaagacac tttccaaata attggaaagc gtcttgaacg ttgatatata
     1981 aaggatatta acgttttgag aattgagatg ctttacgagc tttcttaaga cctggtttct
     2041 tgcaaaatta tattagcctc aactgaaaga tttatttttt tgctccctaa cttcattagt
     2101 aataccatgt ttcattcgat atcttctaaa cgttctacga ttaatccctg ttagctcttc
     2161 aacttcttta tctgtagatc cagtcaaaaa caaattaaat gctaattgaa gtcgcggatc
     2221 attctcacga tattttagcg tacctccctt gtatttccct tttgctttag cgagtgcaat
     2281 tccttgttgt tgcgtttcgc gaatataccg acgttcttct tcggcctggt acttgtatag
     2341 ttcgataatt aagttattaa gtaatcgacg taaattatca tcagtgatac ctgttaacgc
     2401 tggcaaattc aaaacttcca gtgtagcttt cttcagctga attaaattca tcgtttctgt
     2461 tagctcttta ttatttcgtc caagtcgttt taattctgtc acaacgacaa tatcaccttc
     2521 acgtatgaat ttcatcattt cttgaaatcc ttctcgattg gtatctttcc cacttgcttt
     2581 atcactaaaa attttatcac actctgataa taattctaat tgtcggtcta aattttgatc
     2641 taagctgctt actcgagcat acccaatttt cgccataata aaactccttt aaaattctga
     2701 aatccggatc atttttaaat tctgttgccc aactctgttg tcatttttct agataaatgg
     2761 cataaatttt cagcgctaag tgacaaaagc aactaatttt tcatgataga tttttaaata
     2821 tctcaatcta tctccaaaat tagttgcttt tgtagctata gttgactatg aatatgacaa
     2881 ataattagaa aaaactgtgc caattaaata taaaaatgac actcatttac attcaataga
     2941 acaccaagag cccaacaggg tatactctgg tgtcctttta aaaaagtaac tttctgacct
     3001 aattaattga gctactaaaa aagctatgaa atgaggaata aaaaagggat caaaaacttt
     3061 taagtttctg acccttttga gttctaatta tttcttccag aacgaagaat gttatctgct
     3121 ttatctgcaa ttgattctgt tagaaggact gaaaaattta tgccagccct tttgcctagc
     3181 ttatccgccc attgaggaat tgttaaggtt tttctgacaa gttctatgtc tttcaaatat
     3241 ttggcaacat ctacacagat taaaagcatg attttgtatc atagtctatt ataagagatt
     3301 taacaatttt ttatatcacg aattaataat gaaattatca ttgatagtct ttataaattt
     3361 aattgttctt tttctttact gatgtcttcc ccatccataa tgtgacaggt aaaatatatt
     3421 ccttttttgt ttcttcttta tttattcgtt tcatcaaaat agcaacagct gtgtctgcta
     3481 tatcattaat tggttgtatt actgttgtca gatctggtaa tatactccga gtcattaaag
     3541 taccatcata tccgactact aataaatcag ctggtacatt taaccccttt tcttttgcta
     3601 cttgtaatat tagggccgca tcgatgtcat ttgaagcaaa aataccatct attttaggat
     3661 aatcctcaaa catttggaaa aaaatctgtt ttttctttac ataagaaata ttgaaatcta
     3721 atgtcacagt tcgtggaatt aactgatatg ctttcatagt atcttcatag gctagtctcc
     3781 gacgattagc cggagtctgt aagtcaattg gaccgtttgt atgaataata ttttttgcac
     3841 cttgtgcaat taataacttg gttgcaagtt ttccaccatt gtagttatcg gattcaacaa
     3901 ctggaatatc ttcattcatc actcgatcaa ttgcaacaat cggtaaattc tgatatttat
     3961 attcctgaat tccttggtta tgtgtaccaa caattaaacc atctacttga tcagacaata
     4021 actgatttaa ataatttgtt tccttttctt tattattcat cgaattgcct attaatacct
     4081 tataaccaat ttcatataac tttttttcca atgcctcaac gagttcacta aaaaaaggat
     4141 tggctactgt aggaaatagt aacccgacaa tgttagtttt cttttgaaat aattgacgcg
     4201 ctacaacgtt cggttgataa ttgagttctt ctatagcctt ataaacatta tcaatcgttt
     4261 tttgacttaa atagccacga ttgtttaata cacgtgaaac agttgttttt gatacatttg
     4321 ctcttttggc aacatcttct aattttgggc gcattctctt gtctctccta aataatgcaa
     4381 taattaacta tcgaaaaaaa gaactctata gaactactat acctgataat cgatattttt
     4441 tctatgtttt attcttgtaa ttgattcaag cccaaagcaa agtttcctag tgttgccgat
     4501 ccattatttt taattaaagg acgcgtaata tatttactta acggtggtat ttccacgtaa
     4561 tcatccaata atttattaac attaacacga attttttcta acaatttttc atttactacg
     4621 cttccaccaa atactatttt atccggtcgt ataatcaatg ttgtttgaat cacagcttgt
     4681 gctagataat aggccattac atcccaaata ggatccgtta aagaaacgtc ttcacccttt
     4741 tttccaaaac gtgcttcaaa cgttggtccc gcaactaagc cttctaaaca atcaccatga
     4801 taaggacaaa ttcctttaaa atcaatatca tcaggatggc gttttacaaa cgtatggccc
     4861 atttctgaat gaccaaacga tcctaatagt tttccgtcaa caacagctcc accaccaacg
     4921 ccagtaccaa cggtgtaata aactaatgaa tttatttttt cattcgataa agtggacatg
     4981 atgtactctc cataagctga tccgttcaca tccgttgtcc aagacattgg aacattcagc
     5041 tcatcttgta aataacctaa cacattaaca tttcgccatc ccacctttgg tgtagacgta
     5101 atatatcctg attttggtga attttttcga atttctattg gaccaaatga tgcaatacct
     5161 aatgcattta tatcaaactg tttaaaatag tcaacagttt ttttcaatgt ttccctgggg
     5221 gtggtcgttg gacaaattat ttggtcttgt actcggtaat cctcgtctcc cacggcacat
     5281 acaaatttag tcccacctgc ttctatgcta ccaattaaca tcataaaacc tccttactcg
     5341 taagaaattg catctttttt tagatgataa attgtactcg agacaaaagc ttcttcacta
     5401 acactcaaat gcatacttgg ttgattttct gtataataac gctctgtaaa aacaagctcg
     5461 ccatcattaa taaaaatctc tgccgaactt ttatcgataa acactcgaag tgataatcta
     5521 tcacacatct gaatggttcc ataccttgga tcatttttgc cgttacgagt taaggtacat
     5581 tcatttttat ccttagaata aaaaatattg attaactctt catcttttgt ttttaatgaa
     5641 aatttgactt cttgattttt tgatttagtc aatagtacat caattagagc ttctttgaat
     5701 gaagcatctt gagcgattat tatttctcct gccaaattaa ctgtcgtttc aacgccgctg
     5761 ccaactcgaa gttgttctaa ttctctcaca ggtttcatat acaaacgatc tccaattaac
     5821 tctaattctc gaggaagcgt taatgctcct gcccatccgt ctactttttc aggcataggg
     5881 ctttcccaca tatccatcca ggccataaca atacgtctac catctggaga taaagttgtt
     5941 tgtgttgcat agaaatcgtg tcccttatct aattcataaa aatttcctct aatgaatttt
     6001 gctgtctcat aatcataatc accgataaag tagcctgttt catatagatt taaataatct
     6061 tttccatttg cttcaattcc ctgaggggat aataaaagaa tagattttcc atttaattca
     6121 aaaaagtctg ggcattccca cataaatcct tctgtaagta cttccttcgc tttagaaatt
     6181 ggtccaatgt aatcccaatt taataaatct aacgactgat aaacaatagc acgacctaat
     6241 ccatcttttt cttgactacc caaaaccatg taatattttt catcgtgttt ccatactttt
     6301 ggatctctga aatgatgtgt gttatcttct ggtgcttttg ctattacagg atttttttca
     6361 tatttagtaa agtgaatgcc atccgtacta tacgccatgt tttgattttg ccaaaaatga
     6421 tctggatcat tgtctccata atagtgatgt cctgtataga acagatacaa cacaccattt
     6481 ttttcaatag cgcttcctga aaaacaacca ccttcatctt cttgatctcc aggagtcaaa
     6541 gcaagtggta aagattccca atgaactaaa tccttacttc tagcatgtgc ccagtgcatt
     6601 ggtccccatt cagcactata tggatgatgt tgataaaaca catgataata cccatcaaaa
     6661 tagcaaaatc catttggatc attaatccat cctgatggtg cagacacatg atatcccaaa
     6721 cgataacgtg tatttgttaa ttctatagct ctttttgtca taatatattc gctcactttc
     6781 tagataaaat tgttttttat tctgttggtt tccattatcc tcgaataaat ggatcccctt
     6841 gaacatctct atcatcttgt ttaagaataa agaatgcata tatgcctgct agtaatacaa
     6901 taagagaaat aattttaaat gttggttgat agccaacgtt gtctcttaat attcccaatg
     6961 gtgttgataa aatcacttgc ccaatttgag cagctatctg aaatcctatc atatagaggg
     7021 ttgctgatag ctttgtatca aaatgaagtg taaaatagcg aaacattggt aatgtaaata
     7081 atggtacttc taaagcatgc aacattttta tgcatgaaac agataatggt gtcgaggcaa
     7141 acccgcacaa tccaattcgt acacacataa tggttacacc taataacaaa gtatttcgaa
     7201 cccctaattt tctcataatg attggaacaa ttcccatcat taacgcttca caaaatactt
     7261 gaatagcatt caatgtccca tatatttttt caccattagc tgatgttgaa aacaaaccag
     7321 tataaaatcc cggaaacatt tgttgatcga ataccgtata aaatgtccat gtaaaaacaa
     7381 taaagatgat tatcgtccat aattgaggca gtttcattag atcaaacatt tcttttaaag
     7441 aaggaatact atttgattca gcttgttctt gattaaaatt ttgatttgct tcccgttcaa
     7501 ctttagggtt ccaaaataat aaatttaaca atagtaaaga accaaagaaa gatcccgccc
     7561 aaaagtttaa atgaggattt tttacaaata aaaatcctgc caaaagcgct accaaggcat
     7621 aaccaaatga tccccacgcc cttgcttgtc catattcaaa gccaaataaa cgactaaacc
     7681 tttctgacac agcttcaaaa actcctacag aagctaaata tccagctgac aaaaatagcg
     7741 ctcccataat aatgcctaaa ttaaaattgt tatctagcaa tggcccatat atccatataa
     7801 aaaatggtcc aacaagtgtc gctaatacag aagcaaaaat taataaattt cgtttaatat
     7861 acaatttatc ttgtaatgtt ccataaataa acatcaaaat taaggtaact aacgaatttg
     7921 ccgagaatac tgttcctaca gcactgccag ataaccctaa accatttgat tcagatgtta
     7981 gccaaagttg aaaaaatgac caccaaacac cccaagaagc aaaaaatagt aataatgtag
     8041 ctgaactttg taaataagat tgatttttaa atacgttaac ttttgacata tttttatcct
     8101 ccaacgatat atttgtatcc cctttcatga taaccggtta acatttttag aaaaggactt
     8161 gtactcctaa aattataaaa gaaatccgct tacatgtcaa ccggttattc tatttggata
     8221 atgccatata atgtcacagg atatgacttt tattggtgtg tcgcttttct tttttaatgg
     8281 cactattttt ta
//
LOCUS       DACDYM010000093         8228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDYM010000093 DACDYM010000000
VERSION     DACDYM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.094"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IWM59_002366"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IWM59_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9980358.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IWM59_002367"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IWM59_002367"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9980359.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IWM59_002368"
     CDS             2591..3376
                     /locus_tag="IWM59_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9980360.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IWM59_002369"
     CDS             3389..4240
                     /locus_tag="IWM59_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9980361.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IWM59_002370"
     CDS             complement(4364..4816)
                     /locus_tag="IWM59_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9980362.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IWM59_002371"
     CDS             complement(5281..5649)
                     /locus_tag="IWM59_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9980363.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IWM59_002372"
     CDS             5799..6281
                     /locus_tag="IWM59_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9980364.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IWM59_002373"
     CDS             6318..7328
                     /locus_tag="IWM59_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9980365.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IWM59_002374"
     CDS             complement(7443..8156)
                     /locus_tag="IWM59_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980366.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DACDYM010000094         8066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDYM010000094 DACDYM010000000
VERSION     DACDYM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.095"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..883
                     /locus_tag="IWM59_002375"
     CDS             <1..883
                     /locus_tag="IWM59_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9980367.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="IWM59_002376"
     CDS             931..3060
                     /locus_tag="IWM59_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9980368.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="IWM59_002377"
     CDS             3035..3724
                     /locus_tag="IWM59_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980369.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4817)
                     /locus_tag="IWM59_002378"
                     /pseudo
     CDS             complement(4132..4817)
                     /locus_tag="IWM59_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(4953..5147)
                     /locus_tag="IWM59_002379"
     CDS             complement(4953..5147)
                     /locus_tag="IWM59_002379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980370.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5197..5637)
                     /locus_tag="IWM59_002380"
     CDS             complement(5197..5637)
                     /locus_tag="IWM59_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9980371.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5641..6486)
                     /locus_tag="IWM59_002381"
     CDS             complement(5641..6486)
                     /locus_tag="IWM59_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9980372.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6635..8053
                     /gene="pepV"
                     /locus_tag="IWM59_002382"
     CDS             6635..8053
                     /gene="pepV"
                     /locus_tag="IWM59_002382"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9980373.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2480 a         1515 c         1713 g         2358 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagctgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaagctt ttttattttg agcgatcact gcctgatcgg tttgttctag tattttttct
     4501 agtttagctt gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc
     4561 aacttttccc gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa
     4621 gtgccggact gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct
     4681 cgaatcggtg tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt
     4741 ccgggtgcta actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc
     4801 aaattattga taaccattta accttccttt ctatgcagat ataatctatt ataacataaa
     4861 tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg
     4921 cctattagag cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt
     4981 ttgacacgac cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg
     5041 agcagattgt ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc
     5101 attactttaa cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact
     5161 cgacacaata caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga
     5221 agcgtacgct tggaataact gttctaactc cggattgact ttgctcagct tcactaatcg
     5281 ttgcgaatca gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc
     5341 ttcagttccc acaatttcgt agctaaaatc caaccgtgtt cccgtatact tttggatagt
     5401 tgggtggatg ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc
     5461 cagaacgggg atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac
     5521 caataaggct acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc
     5581 catttgatct gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac
     5641 tcatgcctcg ttttccattg attttttaca aaaataattc atccacttcg gaagtgcttc
     5701 gctgatttgt gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact
     5761 atgaagataa acagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc
     5821 tgtgatcatc ccagctaaag tgtcacccat gccgcctgtt gccatagcag cagaacctaa
     5881 aggattggtg tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag
     5941 cacagcaccg atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg
     6001 tagagaactc aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata
     6061 gggtagcaaa agatgattag caacaagtaa acttaaagca gagccatcaa tcaccagcca
     6121 ttgttctttt gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt
     6181 acccaatccg ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc
     6241 agtatccgtc caatctacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt
     6301 tttttcatca gttactacag tcgtgagacc cgctccactg tgtacggcag ctaaagcact
     6361 cataatgatt gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt
     6421 tcctttatgt gaaatctttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat
     6481 cagcatcgaa aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga
     6541 taagaaaagg aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga
     6601 acacgaagac ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt
     6661 agaagcacgt aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga
     6721 gcgcgatgat tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt
     6781 gaaacatatg ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta
     6841 tgcagggcat attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga
     6901 tgtcgttcct gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg
     6961 aaagatcttt gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc
     7021 gatgaagatc attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg
     7081 cagcgatgaa gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc
     7141 agattttggt ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt
     7201 ttctttagct ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt
     7261 ttccggactt cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag
     7321 tgcagatgca gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt
     7381 aagcggaacg attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc
     7441 acatggtgca agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga
     7501 taactatgaa ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca
     7561 tgaagatttt tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt
     7621 gacaatgaat gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat
     7681 caatatgaat ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga
     7741 acaaacagtg gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca
     7801 ttacgtacca atggatgatc cattggtggc tacattattg caagtctatg aagaccacac
     7861 tggagaaaaa ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg
     7921 tggcgttgct tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga
     7981 atttatgagt cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg
     8041 tttggcgaaa taaaaatact aaaaaa
//
LOCUS       DACDYM010000095         7782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDYM010000095 DACDYM010000000
VERSION     DACDYM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.096"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..303)
                     /locus_tag="IWM59_002383"
     CDS             complement(<1..303)
                     /locus_tag="IWM59_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9980374.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVS"
     gene            complement(290..619)
                     /locus_tag="IWM59_002384"
     CDS             complement(290..619)
                     /locus_tag="IWM59_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980375.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(1210..1542)
                     /locus_tag="IWM59_002385"
     CDS             complement(1210..1542)
                     /locus_tag="IWM59_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9980376.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(2378..3418)
                     /locus_tag="IWM59_002386"
     CDS             complement(2378..3418)
                     /locus_tag="IWM59_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9980377.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(3767..4093)
                     /locus_tag="IWM59_002387"
     CDS             complement(3767..4093)
                     /locus_tag="IWM59_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980378.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(4080..4868)
                     /locus_tag="IWM59_002388"
     CDS             complement(4080..4868)
                     /locus_tag="IWM59_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9980379.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(5236..5526)
                     /locus_tag="IWM59_002389"
     CDS             complement(5236..5526)
                     /locus_tag="IWM59_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9980380.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(5662..5841)
                     /locus_tag="IWM59_002390"
     CDS             complement(5662..5841)
                     /locus_tag="IWM59_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980381.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(5838..6188)
                     /locus_tag="IWM59_002391"
     CDS             complement(5838..6188)
                     /locus_tag="IWM59_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980382.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(6181..7506)
                     /locus_tag="IWM59_002392"
     CDS             complement(6181..7506)
                     /locus_tag="IWM59_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9980383.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
BASE COUNT         2353 a         1457 c         1103 g         2869 t
ORIGIN      
        1 gctgacaata taatcactca aatatgacca agtatcttca acagataatt cgtctccatt
       61 atcattcata agcgtcattt taaaccattc attaccttga tcatcttcag gtagcaagcc
      121 gaatccaatt ggattatcag tcaatttttg atcgtataaa tcatcctcgt ctaatgctaa
      181 aacatctaat cctttacaat ttccgcctct tctaaacgtc acaaatcctt tgtaatccca
      241 acgttggaaa gaaaccttaa tcgtatgtgt tccccatcgg ctattcggat cataattttt
      301 catttgttgt aacctcctca taatcagtta gtactataaa ggtatctttg tttttatcca
      361 aaaaatctaa taaatctttt gtagaagata tctcttcatc aacttcacca taataaatac
      421 cttcgataat tgttgctata tctgaaaaac taatttttac tggacattta tcgtctaccg
      481 cattcaatac cgacaaaatt gcttcttttt ctgcaatctc tcgtttgaat ttctttggag
      541 ctaatcgcat gacactcaag tcattttcaa gcttttcaat ccatgcagtt ttactcattt
      601 tcgcaaattt ttgaatcatt cctctaccac ctttgctatc tactaaatta gttttacttc
      661 ataacgaata tttttatcgt ttttcgttca ctcaaaaata tataaaataa atcgactcgt
      721 ttatggcgtc cgaaacgaga aaccctagta gaaaacggta aatgctcgat tgatccaaag
      781 atcaacgtct tcgcaagtat ctacatgtac tgcagcacac gacacaattt cttgtgcgtc
      841 ctccccatta cgagaagacc tactccttat tccatgagtt tgcgtcaaag cctagatccc
      901 ttagtagctg ctcatgatta atctaggcgc tattctaact tgtctaactt ctaaattccc
      961 tcttgtttac tctgtgcata ttatttacac aaattcattt caccactatc caaacaaggg
     1021 catttactcg attgaccaat tttttacgtt accaatcgta aaaacctacc ccgtcacccc
     1081 ccgaaaggaa ttgttgatag atcaagtcta ctccttattt ttctagtgat tctaagttcc
     1141 tttagaagtc tcgacaatat tttcaagcaa gcgaaaaacg ataaaataca agttatgaga
     1201 atgatcaatt cagctgatta aacgccccat cccttttgcg gtacaacgca agactccatt
     1261 ttttcttcga ccaatcagta caccgttttg gtcataaaca tgtttgattg ttggatatag
     1321 ctcttttaaa gggataggat cttttaaaat tatttgtttt tgtttcccaa tgtaataaat
     1381 catttcttct gagactttgt ctttctggat tatatatttc ttacactgca caacggtttt
     1441 actttgacca gttgatagtt tcttcgtgat cttttctgta gcggtacacc gtttaccgta
     1501 gtgacaaata tcttcgagac tcaaaattcc tgtatatgac ataaaataat ccacccactt
     1561 actttaaata tttttaacaa gtttttcttg ttatttttca aaaatagaga attgattttc
     1621 gttcatgaca aacaaaaaag agggaacgca tccaaacatg cgttccctct aatttcagcg
     1681 ttcgtcatac ggcaaagagc cacacggaca tttttataac attatgctta ggcatagatt
     1741 catcgtaata tcacgtattt ttaaccttaa cacagccaaa aaaagtggta tcacttttga
     1801 atatctgtta tactaaatcc gttacgggta ataccgtaac actgaaaggt aaacgcatta
     1861 agggagtgat cttaatgcca cctgtggacg tcgacgaagg ttaccagcct tccgagtctc
     1921 acagggcctt tctttttttt gtattttcaa ttgtccaaat ttatcgtcca aaagtgcaga
     1981 acgaaaatca attcatcaca agcatagcag acaattaaat ttaaaacaac taaaaatata
     2041 caaaaaagtg taattgaata ttagcagcac tgcctgaaaa gaagtaatat caggcttttc
     2101 agcttgggga aataagtgaa ttacctctct tcatgaatta ttataactca aaaaagcaca
     2161 tatgcaaaca ctagttccct atttttttga gtgaaaaata actaaaatat ctaacgatta
     2221 tgaatttaaa acctacgatt gtgattgttt tcataaactt ttaatctaaa aactccggaa
     2281 tactaactat ttatttgtaa tatttgtaat aaaattaaaa tcataaaaaa gttctaattt
     2341 aaaattgatc atttttatag taattgcttt ttatcaacta ctctaaccaa ttttccgtac
     2401 taactgttgg taattcgtta aatgtttctc tttgttcgtt tgcaagcttt gccgcaatag
     2461 ctttttcgac aaaccaattt tttacataaa cgaaaattag attctgcaaa ttgtttactt
     2521 tttcagtttt ttgtttttga taagctttca gtaaagtcat atataatcct ttgtcagcat
     2581 caattccata agattccagt tcttcataaa cgacaatagt ttgctctaat tcttcagctt
     2641 ttttctttgc attatgaata gtttggacgg tttgttgagc ttcactgtaa cttgaagaaa
     2701 atgtttttat caagtttaat acattttctg gtacaaatgt atttatactg tcctcttgca
     2761 tgatgtcgac gaagttatct agcaaaactt gattttgtaa ctggtcagtt tcagtgtcta
     2821 ttatgtgtct attagtgtct atagaattag aatagaatag attgtctgcg attttcgcaa
     2881 ttacggttga gtccttgata tgactggatt ttgccgtatt tgcgattttc gcaattacgg
     2941 tagaatcatt gatttttcgc ggttttgccg tatttgcgat tttcgcaatt acggtggaat
     3001 cagtattgtc ttcctcttcc atgattttaa agacgtcttg attcgtaact tctggcttgc
     3061 ctaagtacaa taaattaggc atagattcaa tcttaccatc acgtttattt actcttcctc
     3121 tcttctgttt cagaagacct gctttttcta attctttttt gatcttagat actttacctt
     3181 cgcggcattg taacaatttc attaattctt caactgtgaa tatgaagaat atattatcat
     3241 cagaatctac ccagttattt ttgactgaat aatcaaatcg atctttaagt agcatataag
     3301 caatttttgc atcatttgaa agatccatgt atttttcgtt agtaaaaaat accttaggca
     3361 tttgataata tttttcacga taaatttctt ttgatttgta ataattaaaa tcattcatag
     3421 ccaatcctct ttctgtagga tagcgttttg ttcaaaacta tgttacaatg aattaaacaa
     3481 atatatctat ccactatata tttttctact gctgataagt cgccaaactt tgttaaagca
     3541 gtagtctttt tttatctttt tttagtttac cttaaaaaat aaattaaagc aaaccgctct
     3601 atttcagggt ttaagctcat ggtaactata tcacgatatt acaataacat tatatcacga
     3661 tatcgtaata ttacaatatg aagataagcg acttatcagc tttgatacta ctgattttta
     3721 taaaaaaaat atcacgatat tatgatatcg tgatattgaa tgtaaattaa attttatttt
     3781 gtttctttgc tagtttatat ccttcaatca agtattcttc aggtatggat tttaatagcg
     3841 catcttctat aacgtctggt ttacttttgt ctgattcagc aatagtcgtc caatatttga
     3901 gtgctgacat tagaattgga tttatacgaa tgctatctgc tgttcttaca ctacttcctt
     3961 gatcttttat gactttactt atatttttct tggttaattc gatttcttct acttcttttg
     4021 gtttgtagtt ctttttattt ttatttccta gtagtctact tactgcagct tgactgcgat
     4081 tatcttctgc catcaaaatc accaacttct tttaaatatt cttctgtaaa tgctttatag
     4141 tcatcttcag ctgttgaggg tttattaaaa ttgattagat ctgatccagc agcttgcgct
     4201 tcgtcaacgg ttattgttct acgaatataa gttttgaaaa tttttgcacc aatgactcta
     4261 gaaatttcgg ttgctgcagt cgtcatttct ttagaaatgt tataacgctt attatactta
     4321 gtaaatagga ctcctcgtac ttcaagcttg tcgttcgtat attcttttac cgtttcaata
     4381 gtttcactta actttattag tccttttaat gagaaatacg cagcttcaac tggaatgata
     4441 atttcatcag aaacagtaaa tgcgttgact gtaagcattc ctaaacttgg tggacaatca
     4501 attataatat aatcatagtt gtccttgatt ggttgaagta ctcttttaag cctgtgttca
     4561 cttcctactt gagtaaattc cctctctgca gaagaaaggg ctatgtcggc tggcaaaaca
     4621 tcaattcctt tatagtccag aatagcatct gtaatgtcag cctccccttt taatacttca
     4681 taaattgtag gttggtttgt tccatctata tcgaaaccta cattatctgt agcatttccc
     4741 tgaggatcaa tgtccactaa aagtacattt ttaccgtaaa caaatttcaa acatgctgct
     4801 aaattaacgc tagtcgtagt tttagaaact ccaccttttt gattggctac tgacaatatt
     4861 cttgccatta aaatatcccc cattcatctt atattataat ataattatat cataatattg
     4921 tgatattgca atatcgtata tgcattatat tgtaatattg tgtgattatt ttgttttaaa
     4981 gatatatatc ggttcggata taaagattat tcatatgtta ttcgaaagta aaataatcac
     5041 tttttattac tttaaaattt tttattaatc ataagaaata tgttttgcga gcagcaacta
     5101 gaataattta aacattatta aactgtctat attgttacta ataattccct tcttatagtg
     5161 cattttctta gaaaaaaaca atattacgat atcatgatat taagatatcg taatattata
     5221 caattgtgat tgatattata tatgtttttt tattttttta tcatgtttaa tcttgaagga
     5281 atcatattct ttttctaaag acaggagctt ttcataaaca tcagacatgt ttaaactaga
     5341 ggccaggctt aaatcataaa cgaatgaagc aggtaatgat tttattctac gttgtcgggt
     5401 tatccagctt gcaaccgttc cttgtgggta ggcatataac tcacaaaatt gttctacagt
     5461 gattcctaaa ttcttgataa tgtaaacatt gatcgggtgc gagtaaacaa atgtattgat
     5521 agacatggtg cgtaagccta ctttctttta ttattgcgtt tgcaataata gtttaacaca
     5581 cgcattattc tagtatctac tcctatttgt aaaaacaaaa aaggattcat agagataact
     5641 ctatagaatc cgtaaaatac cttatatata taacgggaaa atataaaacc taaaattatg
     5701 ataacctgct gcagcttcta catagaaaga gaagcgctca acaccaacct catttatcaa
     5761 acgttggcca taaccccaaa tctcttctga tagctgatca aaggttatat actttagctc
     5821 atattgctgg ataatgttca ttctaatcga ctccatttat tatcctggta aaattcgata
     5881 tgacgtattt cgtcgtagtg aattttgtct tttccaacgt agatccccag ctcatcacta
     5941 cctaagatct tgccaacaat atcttctgga tagtatcctt cagcgtttcg ttcttctttt
     6001 tgaatagata ctgatctatt ttttatgact gcagcgttca atacttccgc aatctcttca
     6061 gtcgtcattt gttcttttgc taaattttga ctggttcgtt gttcattgtc ggcatcaatt
     6121 tcttcagtat gatcagataa ataaaaccct gcccacttta aacgtccacg atcattgtaa
     6181 tcaatcattt ttaattcccc catttccgcc cgcatgacca ccaacaagtg tgcttctagt
     6241 aatgcttcta gcgctttcta acattgaaga agcatgtaca atcgacgtaa aaccatattt
     6301 ttgccgaatc ttgtcaataa ttttatctaa attttcttcg ttaatttgct tttgtggatc
     6361 actaaataaa tccagctgaa gcgaatcggt gtacacaagc ttgctatagg ttattccaac
     6421 gtgccttact tcttgtcctt cataatactt atcaaacaag aaaagacaat aattctgcaa
     6481 ttcctttgta ttatctgtga taggtatttt catctggtgt gaaaatcctg gtctcgtttc
     6541 taagatcgac gtaccaatgt ttaaatgaac acatcctgtt ttacaattat gctgcctgat
     6601 acgaatggct acttgttccg ccatttcttt taccaccagc tcgatttcat ttcttctagt
     6661 ataatctcgt ggtaacactt gtgaattacc ataagatttt tcctttgtcg gttctggcgg
     6721 gattgctata tctgttcgat cgattccgtt agcatggaag tacagctgta atccaatcac
     6781 acctaatcta ttcttaatcg tataaggatt ccagttagca aggtctctaa tgctcaaaat
     6841 tcccatttgg ttgagccttt ttttcattcg agaaccaatt ccccaaaagt ccgtcatttc
     6901 gggaatattc catactttct caggaacgtt ttcgtacgtc cattccgcaa taaatccttc
     6961 attatgcttt gcttcattgt ctaaggctaa tttagccaaa aggggattat cgccgacgcc
     7021 taccgtagca atcaaaccta actcttcttt aatacgttct tggatcattt gagcgagctt
     7081 cttccttcgc tgacttcgtg tttcttcagt cgtaaaaaga ttcagtgatt ttgtcacttt
     7141 taaaatcgat tcatcgatcg agtaaatcag tagatcttca tcagccacat atcttctgaa
     7201 aatattattt acctgcatat ttcgcttgat atataggttc atacgtggtg gaacaacgtg
     7261 tagtgtttta ggaaatggtt gtggtaagtc acgtggtcta ctcacatttg taataccata
     7321 ccgctttttt gcttcaggag aagaagctaa tatcaatcct gaaccagtat tatcagatcg
     7381 actcataaca acaagttctg ttgttaatgg atctaaattt ctttctatac attcgacact
     7441 tgcgtaaaag cttttcatat cgattaaaaa gtaatcattt actggctctt ttgaataatc
     7501 catcataaat aacactccat tcttccacat tatacaaaca tatgttcgat gttgtaaaga
     7561 agaaatagct aaatacataa aaaaagaagc ataggtatgc tatcaccctg cttcttctta
     7621 tgatttgtat gatcaattta ttctatcact ttactaatta atttgaaaat ctaataaaaa
     7681 ctctcactct aaaagagtga gagactgcaa attagagtac ggactttttt ggagttccgt
     7741 tctttaatat taacaaatga ataaaatcct tacaacattt ga
//
LOCUS       DACDYM010000096         7626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDYM010000096 DACDYM010000000
VERSION     DACDYM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.097"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWM59_002393"
     CDS             complement(14..706)
                     /locus_tag="IWM59_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9980384.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IWM59_002394"
                     /pseudo
     CDS             791..967
                     /locus_tag="IWM59_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IWM59_002395"
     CDS             complement(1143..1712)
                     /locus_tag="IWM59_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9980385.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWM59_002396"
     CDS             complement(1786..2109)
                     /locus_tag="IWM59_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980386.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IWM59_002397"
     CDS             complement(2263..3075)
                     /locus_tag="IWM59_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980387.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IWM59_002398"
     CDS             3429..4277
                     /locus_tag="IWM59_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9980388.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPLR"
     gene            4422..5363
                     /locus_tag="IWM59_002399"
     CDS             4422..5363
                     /locus_tag="IWM59_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9980389.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IWM59_002400"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IWM59_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1370 g         2472 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattccttt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctt
//
LOCUS       DACDYM010000097         7597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDYM010000097 DACDYM010000000
VERSION     DACDYM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.098"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(135..425)
                     /locus_tag="IWM59_002401"
     CDS             complement(135..425)
                     /locus_tag="IWM59_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980390.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IWM59_002402"
     CDS             complement(543..803)
                     /locus_tag="IWM59_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980391.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="IWM59_002403"
     CDS             complement(940..1353)
                     /locus_tag="IWM59_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9980392.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="IWM59_002404"
     CDS             complement(1809..2294)
                     /locus_tag="IWM59_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980393.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2648)
                     /locus_tag="IWM59_002405"
     CDS             complement(2433..2648)
                     /locus_tag="IWM59_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9980394.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="IWM59_002406"
     CDS             2872..3672
                     /locus_tag="IWM59_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9980395.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="IWM59_002407"
     CDS             3691..5559
                     /locus_tag="IWM59_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9980396.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYNILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="IWM59_002408"
     CDS             5552..6043
                     /locus_tag="IWM59_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9980397.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="IWM59_002409"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="IWM59_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9980398.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="IWM59_002410"
     CDS             6603..>7597
                     /locus_tag="IWM59_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9980399.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2234 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacaatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DACDYM010000098         7314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDYM010000098 DACDYM010000000
VERSION     DACDYM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.099"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(173..1867)
                     /locus_tag="IWM59_002411"
     CDS             complement(173..1867)
                     /locus_tag="IWM59_002411"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9980400.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            complement(1924..2052)
                     /locus_tag="IWM59_002412"
     CDS             complement(1924..2052)
                     /locus_tag="IWM59_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9980401.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2051..2299
                     /locus_tag="IWM59_002413"
     CDS             2051..2299
                     /locus_tag="IWM59_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980402.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2491..2856
                     /locus_tag="IWM59_002414"
     CDS             2491..2856
                     /locus_tag="IWM59_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9980403.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            2853..3218
                     /locus_tag="IWM59_002415"
     CDS             2853..3218
                     /locus_tag="IWM59_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9980404.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(3310..4737)
                     /locus_tag="IWM59_002416"
     CDS             complement(3310..4737)
                     /locus_tag="IWM59_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9980405.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            5207..7042
                     /gene="tdc"
                     /locus_tag="IWM59_002417"
     CDS             5207..7042
                     /gene="tdc"
                     /locus_tag="IWM59_002417"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9980406.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         2287 a         1245 c         1443 g         2339 t
ORIGIN      
        1 tgggtgttct tattacttga tgcagggcag ctttttcaca acctcttggt aaacggtgtt
       61 caaaagcttg catctgcggt aataagtcca accaaatcaa aaacataaga agctgtttta
      121 gcctggttgt tcttattact tgatgcaggg cagctttttc acaacctctt gattattcaa
      181 acattttttt tgcggcacga gctagagtag gaatatcttt gtcgtaacgc ggtgttttga
      241 caagacggga cataggaata cccgcaaaat ggaaagcagc gatttctcca gaacgtacag
      301 cactttgttc agtaaagatc atctgataag gctgctcgac aaattctcct gtaaaggcca
      361 gattggtcga atgtttagga atgacttttg gtcgatcgct tttggcacga ttattgaaca
      421 acgcggaagc ataaggcata tagacaggga tattatttat tacgctgtcg aaaatttctg
      481 tttctttttc acgaatattc actggtccag gatctacttt tgaaagttgt ccaatcagtt
      541 cttctagcat ttcttttcca ttcattttaa tatactgttt atccacaaat tcgccacgtc
      601 gtcttggata aaggaaataa ccccaaataa ctgtttcatt tggtttctga gacgtaaaat
      661 gcggttgatg atgcacgacg atagacatat tgacatcttt ttggcctaac ggcgtgatcg
      721 gtgtggttga aataaatgaa ttcaaggcat ttcctggttc ttgagtcgtg atacgggtga
      781 tttcattcaa caacaaatgg tttttcgtcg tcagggtaaa gctgacccat tcgcttgcat
      841 ctcgatcagc aaagaatttg tcaggatttc ccaagttgta gaaacgttca ctggcttttt
      901 tccagagact agaagctgca ccataatcca tattttctac agctggtgtt tcgtaatctc
      961 ctaaagtagc tgaatctgta atggacccat tagtgaaaat aactgctgta tcttcatcga
     1021 ccacaacgta ttcttcttct tgagtcagtg tattggtaat tttcaaacca gtcacagtga
     1081 tttcatcttg catcggtgtt tctttgaatt cccagtcagt gacacggcgg ttcaaaatga
     1141 ttttgcatcc ttgttctttc aagtactcga tcaaaggaag cataatactt tcgtattgat
     1201 tgtaacgtgt tcgattgacg cccactagat gttcgatttg agtgaattca taaatcattt
     1261 gatgcatata gcgacgcaat tcttgagcag aactacgtgt tctgaaagca aatgttgttt
     1321 cccacataaa ccagaagttt gtttcaaaaa agtgtggatc atctttgaaa tattcttcga
     1381 tagtgatatt gtctagtttt tcttcttctg aatcaggcat agcaatcagt ttagtcagaa
     1441 gaatacggtc tttattattg aatcccagtt ttccgccatc aatgattcct ttgccacctt
     1501 ccatcaaacg tgctttgtca aaggttcggt gttttgcatc aaaatctctc gtatcttctt
     1561 cagcagtcat ccctggctca gtaacagaag gtattcgact taacagatcc atcaaatcta
     1621 cataggtacg gtaattaagc atgcgaccgc ctcgagcaat atagcctttt tggttttcta
     1681 atggatggtt tttattccaa tattcatcca ctacagtgtc ggtcggtgca ccatcatttg
     1741 aaccgtgatc atccaatgaa taaaaggtga tctgatcccc tttccatttg ccttcttgaa
     1801 tcaaataaac agccgctgcc atattggcaa taccggcacc aatcattatt gctttacttt
     1861 ttctcattgt gattcctcca atgtcttcca agtatgtccg tagtgattca actaaatagg
     1921 atgctagatt gattcgaatt catcatataa atgactgtca tcttcgaata gtcctgtctt
     1981 ttttcctaga tatgcagcgc caagggcaag caaacctaaa ccgatgccga ctttgattaa
     2041 ttttttactc atgaaaaacg cctccttgtt ttctctttct atatccaagg atagttcgtt
     2101 tttattagaa tgccaaatga taggacgaga agaaaaagga aattttgaca aaaaagaaga
     2161 agtgtctgat tttcctgtaa ataaaaatag ggtaggtaag aagaaaagat ttgaggaggt
     2221 gtatggttct atttttagaa atttgggtac aaaaaaagca ccgagacagt gcttctttga
     2281 aaagctgatg acggactaag ccttatttta acttgctacg ttgttttgga tacttccacg
     2341 atttatggat ggagggagaa aaatgggaga gtgaaaaaga aaaatttaag cgaaccgtta
     2401 tcattagaac aacttttaaa tagacgaaca aggtaacttt cttttacaat aaaagaatag
     2461 gaacaaatta aaagaaagta ggtagcgtat atgtttcgct caaatttaat tgaatttcta
     2521 gaatggatca ttgctatttt aaatatttgt tctatcggta tcttaattgt cgggatcgtt
     2581 cttgttctat tcaaactttt tgattggtct catgtcaaga aaaactatgc agaacgtaat
     2641 actcgtaatg cagatacaag aaaactgcta gcaagttata ttttgttaag cttggaagta
     2701 ctagtcgttg ctgatatcat cgagtcagtt atcaagccga cttggacaga tattttgaag
     2761 cttgcactta ttattatcat tcgaacagtc atttcttatt tcttgaatca ggaaatagaa
     2821 gacaaagaaa aacaaagaag aggagaggta ggatgacgac agagttgatg cagtttcttc
     2881 ttccatattt cgaaaaactt gtactgggac ttgatatctt tgctatctgt ttattggttt
     2941 ggggtgtttg tctatcaatc aaggattttt tgaaatattt atttgtccat acaaaagtgc
     3001 aaagcgaccg agtcagcgag aacaatgcta ttaaaaagat gttgggaggc tatatacttc
     3061 taagtttgga aattcttgtt tcagctggga tcatcgaatc gattatcaaa cctaccttgc
     3121 aggatatttt ccaactagcg gcgttggtag ttatccgaac catcatttct tattttttaa
     3181 ataaagagat cggacccgta gatgtccaaa ataagtgagg ttgtgagcct gacgtgtatc
     3241 aagagattgt tacagaaatg ggcatcttca acaaataaga ggctgtgaca tgatttttgt
     3301 cacagcccct tatttgtttt gcacagctga aatgatagct cgatcgatcg tattgatcga
     3361 aataggaata atattttcat attggaccgc atctggtgta tataatgtac gttcacataa
     3421 tacccggttt gaggactgct gcgcatactt ctttatatca gatatacttg aataaattgt
     3481 tggtgcaact tgaaaatcga aagtagttac agcttccaag gcttcccgat aaatcagata
     3541 atgaacatcc gaatttgtga cgatatagat agtcagatgt tcttttgtca gacggagaat
     3601 tccgctcaat tcgatcacaa aagcccgcat gcaattagga ttcaaccgta gatcataagg
     3661 taactggctg gaccagctgg aaaaaattgt ctggacttct ttatataaat ttgtctgttc
     3721 ctcatttaaa agataaaatt tttctggcat gaataattga tagttatccc aagccgaacg
     3781 catcagatga atcagcgcac gttcgaacgg ttggtttccg aataattctt gattgaagtg
     3841 agattcaaag gtagcaacta gttcttttat ttcgggagta ttgtcaataa acacttggtg
     3901 tagctgttga aagtcttccg caatcaactg ataagaatgg aaaccataat tacaaagatt
     3961 gaatagtgtc aaaataaagg cgaattcttc ttttttataa taggtttgga aatcagtgtt
     4021 ttcccatgct gattcgacat aattgtaaat aggacgtttt ttgatttctt cgataaattt
     4081 tgaatctatt gtcacagcac acacttgctg gcgtaggaaa ctgatagcaa aaccaatcga
     4141 taagatttct ttattttctt tatcataaaa tctgccacta ttttccgtta cggattcaat
     4201 gaaacgatcg gcacgttcaa aaaacgattc aggtagctcc caagaattaa aaccgcctaa
     4261 acgatacgac acattaagaa gcagcagacg tctttccatt tcagtgaatc gaggggagta
     4321 gcggtcgatt tcgatcccgc agtcattaaa ataagctaat acttgttttt ttaatgaata
     4381 agctttgctg acagagatga attcacattc agttaattga agataatcag gttcatcttc
     4441 ttccaaataa agcagacaag tttttaaaaa caacgaattt tgagacaatt gctgcatgat
     4501 cttgcatcga ggtatatgtt ctgaaaaata taaagtgtaa cggtcctttt cccgatgtgt
     4561 ataagcgatc tgttcttcaa attctttttt tatgttattg atatcattga gtaaagtggt
     4621 tttgcaaaca tgcaggagat tggagagctt ttctagattg acttctttat ttacatagaa
     4681 ttgcccacat aaaaacagct gtcttaaaat atttttctca atatattctt caatcatagt
     4741 agagcctccg ccttgtattt ttttgcacaa tatgggaaat attatgacat gttttctttt
     4801 tgaaatcatc agttaatttc tgaaaaagca tataaatgcc gttaaaaaag gttttttatg
     4861 ttataaaatt tggaaaacag ataattctat taaaaaataa attgcgaatt tttaacaata
     4921 taaaaaaata aaaatatgtt tagtgaatga aggacagtca ttttatttgt ttgttcgctt
     4981 tttttaggtt atctatttta gaagactgat ttattgaaaa attgttcgct tttttgcgtt
     5041 gaattatcag aaaaatccga ctttatttat ttttgtttgt aaaaatctac gtggatttga
     5101 tgttataaaa aaaaaattct catttttggc gctatagtga agaggtgtta cagataaaca
     5161 catgtatctg caagacattg accaacaaaa tatggaggga agaaaaatga aagatatgga
     5221 tatcaaggcc gtctttatag gcgacaaagc tgaaaatgga ccagtttata aaatgttgtt
     5281 gaacaagatg gtagatgaac atcttggatg gagagaaaat tatattcctt cagatatgcc
     5341 tgcaattagc gaaggagata agctcactcc agattatcta gccacccgcg accatatgat
     5401 agaggtcttg gatgaagtca gtcagcgatt acgcgcagga tcgatcccat ggcattcagc
     5461 aggccgttac tggggacaaa tgaatgctga aacattgatg cctgctttat tagcttacaa
     5521 ctatgcgatg ctgtggaacc caaataatgt tgcattggaa tcttctatgg caacttcgca
     5581 aatggaagca gaagtaggac aggattttgc ttcattattc aatatgacag acggatgggg
     5641 acacattgca gcagatggtt ctattgctaa tcttgaagga ttatggtacg cccgttgcat
     5701 caaatcgatt cctttagctg tcaaggaagt gctgcctgaa aaagtgaaaa aaatgtctga
     5761 gtgggaactt ttgaatttat ctgttgaaga aattttggaa atgaccgaaa gctttacgga
     5821 cgaagaaatg gatgaggtca aagcagcttc ttcccgtagc ggaaaaaata tccaaagatt
     5881 agggaaatgg ctggtccctc aaacaaaaca ctattcttgg atgaaggcgt tagatatttg
     5941 cggagtcggc ttagatcaga tggttgctat cccagttcaa gaggattatc gaatggatat
     6001 taatgctttg gaaaaaacaa ttcgtgaatt agctggacaa aaaatcccaa ttctaggtgt
     6061 ggttgctgtc gtaggaacga cagaagaagg tcaagtagac agtgtagata aaatcgtcca
     6121 attaagagaa aggttaaaag atgaagggat ctatttctat ttacatgttg atgccgcata
     6181 tggcggctat gcgcgttcac tgtttctaaa cgaagcagga gaatttgtgc cgtatgcttc
     6241 cttagctgaa ttctttgaag aacatcatgt tttccaccac tgcgtaacga ttgacaaaga
     6301 ggtatatgaa gggttcagag ctatttcaga agcggattct gtcacgatag atcctcataa
     6361 gatgggctat gtcccttatg ctgctggagg aatcgtaatc aaacataaaa atatgagaaa
     6421 tatcatttct tatttcgcgc catatgtgtt tgaaaaatca gtaaaggctc cagatatgtt
     6481 aggagcttat attttggaag gatcaaaagc tggagcaacc gccgccgcag tctggacagc
     6541 acatcgtgta ttgccattga atgtgaccgg atatggtcaa ttgattgggg cttcaatcga
     6601 ggcagctcaa agattcagag aatttctcga tcatctgact tttactgtta aaggaaaaac
     6661 aatcgaagtc tatccattga atcatcctga ttttaatatg gtcaattggg tattcaaaga
     6721 acaaggctgt acagatttga acgctatcaa tgaattaaat gaaaaaatgt tcgatcggtc
     6781 ttcttatatg gatggcgatg tttatggtga acggtttatt acttctcata ctacatttac
     6841 acaagaagat tacggtgatt ctccgattcg ttttgttgaa agaatgggtc taacgaaaga
     6901 agaatggaaa aaagaacaga aaatcactct gctacgtgcg gctatcatga ctccttattt
     6961 gaatgatgat cgaattttca atttttatac aaaaaaaatc gccaaagcaa tggaaaagaa
     7021 actaaacgaa atcatccaat agaactgatt tttgtgtgaa agaataacaa gagacatatg
     7081 gagaacagac aattatttgc ttcatcaaaa caattgtatt tccccctgtt tttcatatat
     7141 tcctcgccaa gatattcttt tttataaaac gttctataag ttggatcgtt cgtttcatgt
     7201 atcaaaccca gttcactaag atcgttcaat ggatgtttcg gtgatactgg gttttgtact
     7261 gaatcgatga aaccttgtca tttctgttct tgaacaaaaa taaaagaaaa caaa
//
LOCUS       DACDYM010000099         7189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDYM010000099 DACDYM010000000
VERSION     DACDYM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.100"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(5..829)
                     /locus_tag="IWM59_002418"
     CDS             complement(5..829)
                     /locus_tag="IWM59_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9980407.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(802..1482)
                     /locus_tag="IWM59_002419"
     CDS             complement(802..1482)
                     /locus_tag="IWM59_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9980408.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1505..2443)
                     /locus_tag="IWM59_002420"
     CDS             complement(1505..2443)
                     /locus_tag="IWM59_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9980409.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2688..3539)
                     /locus_tag="IWM59_002421"
     CDS             complement(2688..3539)
                     /locus_tag="IWM59_002421"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9980410.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3693..6266)
                     /gene="mprF"
                     /locus_tag="IWM59_002422"
     CDS             complement(3693..6266)
                     /gene="mprF"
                     /locus_tag="IWM59_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9980411.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6407..>7189
                     /gene="proC"
                     /locus_tag="IWM59_002423"
     CDS             6407..>7189
                     /gene="proC"
                     /locus_tag="IWM59_002423"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9980412.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKD"
BASE COUNT         2196 a         1587 c         1240 g         2166 t
ORIGIN      
        1 taatttagtt attttttctt ctgaaggcaa ccaatattcg tttgatgata tttacaatca
       61 ggaacaaagc aataaagact aatgtgattg ttgctcctgg aggtgtatct agataaaacg
      121 agctagttag tcctgacagc atccctagaa atcctacaca aacactgata gcaatcacga
      181 cattgaagct tttgcctaat cgcataccga ttgctgcggg aagaaccatg attgcggata
      241 tcaaaagtgc accggcaatc gggatcatca cagcaatagc cacaccagta atcacattga
      301 acatcatcga catcaaatga acgggtagcc catccacgtg ggcggtatct tcatcaaaag
      361 tcagaacata catcggccgt ttgaataaaa agaacaatat accgatggca acgaataaga
      421 ttcctaaaaa gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac agataggatt
      481 ggatacttgt tgctgaattc cctccgctca aattcatcaa tactaacgct agcgccaaac
      541 caccagccat taaaatggca atggagattt ctgaataggt tcggtagatc gttcgcagat
      601 actccaatat cacagctgct aatacaacga ctagcatggt cgtaagtgta ggattaaggt
      661 tcaaaaagaa tcctaacgct acccctgcta aagagacatg agaaagcgta tcagccatca
      721 gcgattgtct tctgatcact aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga
      781 tagcggcaag aaacgctctt ctcatgaatt catatgaaag caacgccatt gagaatcctc
      841 ctttctcacc agtcggatct gtctgtccgc atactgtttg atatcttcat gatcatgggt
      901 gatcattaga actgatttcc catggacatg cgcactatgt cggagaagac gataaaattc
      961 atttctggat tcttcatcca tacctgttgt tggttcatca agaacaaata agtcagggtc
     1021 agtagcaaaa atacgcgcta aactgattcg ctgcttttgt ccgccagaca attcacctat
     1081 tcgccgattt cgcatatccc acatccctac agcttctaaa gcacgctgta catgttgatg
     1141 atctttttca gagagacgct tgaaccagcg gcctcttgga tatcttcctg aacggactaa
     1201 ttcgatcacc gtactaggaa aacctgcatt gaaagatgca acttgttgag gaatataacc
     1261 gatacttaat ttttttcctt cttgattttt ctcagcaatc tttatcattc cttttgaagg
     1321 tttcagtaat cctaacgatg ctttcaccaa ggttgacttg gctgctccat tttctccagt
     1381 taaaatgaca aattcacccg catctacatg ataagagaca tcttctagta caggttcatc
     1441 atcatagtaa aaggtcaaat cagatatttc gatataacgc accttacaac ctccattcta
     1501 aaacttaatt gatactttct tttaaagctt tcagattttt ttccatgaca gaaatataat
     1561 tttcgcctgc tttgatttgt tcattagtca agctttctaa aggattcaac acttctagct
     1621 tcactcctgt ttcattcgac aatgtctcag ctaccttcga agaggcattt tcttcaaaat
     1681 aaatcacttt gatgttgttt ttcttcacaa attcttttag ctcagctaag cgacttggtg
     1741 tgggttcctg atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg tattgattcg
     1801 ctaaataagc aaaagcagca tgctgagtga caaatgtgcg gtttgttgca tcttttagct
     1861 catcggagta tttttgatcc agtgcttcaa gctttttgat ataagcatcg gcattcttcg
     1921 taaaagtttc ctttttatct ggatattttt cgcctaattc ttcagcaatc gttctgactt
     1981 cttttatagc taataccgga tcgagccaaa catgtggatc gtattcatgt gaatgaccat
     2041 gcccttcttc tgattcttca tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt
     2101 tagctgcttc gatcatcaag gtctgtttgc tatccactga atcctttaac gaatccacaa
     2161 aaaattccat gtctgaacta ttatacacga aaacatctgc atcggaaatt tctgctcttt
     2221 cttttgcact tggttcgaaa tcatgcggct ccgttccagc agggatcaaa agtttcacgt
     2281 ttccttcatc ccctacgatc tcttttgtaa aatcatacat cggataaaaa gtcgtaacga
     2341 tcgtcaaatc ctcttttttg tctgcttcct tgttcgtatt tccacatgct gcaaacagta
     2401 aagcacctat cattaccgta gctgtcaaaa gatatttttt catttatatg ctcctttcaa
     2461 tgaacaaatc ggaattttac gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca
     2521 cagtcaatag aaatccatca atttctgatt taagcatgta tattcttgat taaatcgtaa
     2581 aatggatgag ttgctgctca tatctagcca tttcactaca ataaaaaact gaagaatacc
     2641 attttaggat tcctcagttt ttcatcacct attttacttc ttatatttta ttgcagggtt
     2701 cgtactaaat aaacttcctc acaaaaacct tttaacccat tatatactct ctgcgctctt
     2761 gaataatgtt gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct
     2821 gctccgtact tgatcatacg ctctttgatt tgatggatga ctggatgccg ctttcccgtt
     2881 atatcttcta aactgtttcc catttttttg atcatctgct gataatcttg ctctcggatg
     2941 gcttcagcaa gtccgtggat gtcttgatgt tggatggctt gcgtatctac ttctctaaac
     3001 actgtacgtg tagagacact cattcttggt ttgaccaaca caacccagca ctgtggcata
     3061 gaaggtaata cttggatttc ttcccctttg ccaccgacaa atgccgtact tccgtagaca
     3121 cagtaaggaa catctgtacc gacttccaaa ccgatttctg ccagttcttc taacgaacag
     3181 cccagattcc ataattggtt gatccctctt agagcagctg cacagtctgt acttcctccg
     3241 cctaatccag ctgctactgg aatattcttc ttaacaaaga tcttgacacc ggttttgata
     3301 ttgtatttat tttttagcaa taaagcagct tgataaacat tgttccgttc atctaccggt
     3361 aaaaatgctt tatttgtttc tacaatgatt ttgtcttctg ttatttcttc aaaaaataga
     3421 tgatctgcca agtctacgct cgacatcacc atttccaact catgataccc atcttgccgt
     3481 ctatataata catccagtcc taaatttatc ttggctggcg ctttttcaat caattccatc
     3541 ctctcaccca ttcgaaaagt ctaagtcgca agtattctat catagggaaa aataaaagta
     3601 tagcttatgt ctgcctagtg gttgattttg tcatcgtttc aacagctttc acagaatttt
     3661 ctcttcataa tccaaacaag ttctagtgat tcttataatt ctgtgagcac ttcttctgcc
     3721 aaactcggaa catttccttt ttcttttcct atcagactga gtagctgcat ggtagcaaat
     3781 ccgaaattac ttcctttcct ataagcaaaa taacgtcctt cccaatgatc gacatatttt
     3841 aatttctttt cataagcaga ctggaaggaa tagaaaaaat taccataatt atatacgata
     3901 tgcatgactt tttcttgaaa gaaagaaaaa tctgtttctc caacattagc taatggagct
     3961 aagcctagat tggcttccga gtagcccatt ttctcgtata caataaacag attggctaac
     4021 agatagtcag ctaattcctc agattcttta ggtaagaccc gcaacaaatc atatcccgcc
     4081 tgcttcttcg tcatcggctg agcggtgatg aagccaacga ctttttgatc tttacggaca
     4141 ataccgacac cgctagataa tacatatgat ttatcaaatc gtcccacaga gaagtatttt
     4201 tctcgtgcac cttgaagcca tttttgtgaa acttcattta cagcttgatg taattcttcc
     4261 ggcagttgat cgtaccaagt aaaatggtat ccttctttca tcaaacgttg atattcaagt
     4321 tgctgactca gccgagacaa gtcttctgtt gctagatcaa caatccctgc ttcacctact
     4381 ttcgtgaagt gaaaacccaa ttcatgaagc atcactacgt agctttctga tatcttataa
     4441 aacgcaagct gatagcctaa acaatctgct tgtttcataa actgattcgt tgctgcccgc
     4501 atcattttag gattgccaac tggatctcct aaaacaaagc atttgtttcc tttgatctga
     4561 taaccaaaaa cgacttgatc ttggtcgtct tcttggtaat aaaaataact gtaacctgac
     4621 aagcgaagct gatggcttgt agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg
     4681 tctttttcgt actcactgcc aagcttcgta gtatcttcag ctaaatattg ataaagcgcc
     4741 actaaagcta gcaatgagat gcccaaacca ataagccctg agaaccacac atcatctgaa
     4801 ggaaacaaaa taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag
     4861 cctgcaattg cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa gattatagct
     4921 ccccaagaat aaacgaattt tgttcgataa aattcttttc tggataacca cacagctaac
     4981 agaatcaaga catataccaa tatcaactgc caagataacg tacgtgtgat tgtatttaaa
     5041 ataccaaaag atagtaaagc aatcgtaggc agaaatgcct ttttgacttt tactgaaagt
     5101 gctctagcaa ggccgagtaa taaaaagcca accaatagat tcactgtctg atctaaaaaa
     5161 ttgaatgaga acggcaataa tacttgaaat agccgactga ttgcagacag attagtgatt
     5221 gttgatagaa gtaccatcat gatccccgca aaatagacag cacttacgag cacgatatgc
     5281 gcaacctttt ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat tttgactcct
     5341 gtatgtgtga tgaacaatag aagtcccgtc ataaaaggta gaacataata aaacaaccga
     5401 taatataata gccaaactac tgctacatct tgccgaacag gcaattgact cattcctaag
     5461 atcatcaaaa cgtcaaaagt ccccattcca cctgggacca tcgtcagcat tcctatcaat
     5521 gtagcaatca tgaacatagg atagatcgaa agcatcgaaa tatctattct catcaacttg
     5581 ccgatgatca aaaataccgc aagggctccc gtccattgtc caaaggatgc cagtagcaga
     5641 ccaagcaagc cttttggata aaactcgaag aacaatgtct ttctctttag ataagcaaaa
     5701 aagaaaatga ctggtgctaa aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa
     5761 atactttctg actgaataaa aaagacatca ataaatgtaa gaaacgacca tacagacaaa
     5821 ccagtcagca taaatagtgc gacttttgat acagtggcta aaaccatttt tctatttgtc
     5881 tgctttccat aaaaactagc ccgcagactt gctcctacca caccaccaaa accggcgaga
     5941 ttgttgatcg tgtttgttac ccacgcagca agaagaaact cttttctttc catttttggt
     6001 tttccttgtt tttctaagac tctgatcgtc acccaatcat acaacaacat cggagtgaca
     6061 cccagcagtc cagccagaat catgagcagg atcgtcatgc ggctttgttg tcccatcctt
     6121 tgccaaatat cttgccaact catgccatta gcaatatttg cgacttgatt tgccacaaag
     6181 actaacacgg agccgaaaaa aatcagtttt aaaatcagac tatgttcctt tatccattgc
     6241 agcatcaagt atcgtttatt tttcacattt tcccattcct aacttgtata cttttgtgtc
     6301 ataccccaat gttatcttat ttattttaat ttttccactt tagacctgtt ctttgcttgc
     6361 gatgatagaa aaagaaagat atcctaatgc taaaggagag gataacatga aagtaggatt
     6421 tatcggcaca gggaatatgg cacaagctat tattgatggt ttattaaaaa aaggaacagt
     6481 ctcgaaagaa gaggtgttcg tttccagtgg acattttgaa aatgcacaaa aatttgctgc
     6541 tgaaactggc gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt
     6601 tcttttagcc gtgaaaccaa aaatcattga aaaagtattg gaagaactaa gagaatatag
     6661 ccagaaagtc ttattcattt ctattgcttc aggaaaatca ttaacagatt tagaaacagc
     6721 gcttgaagat cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg
     6781 agcaggcgtt tctgcgattt gcaaaaatga agctgccacg gaggaacagc ttcagcaagc
     6841 tacgatgatt tttgaagcaa tcggcagcgt ctatccttta gcagaatcag atttcagtac
     6901 attcattggc cttgctggca gctcacccgc attcgtttac atgctgatcg atgcgatggg
     6961 acggactggt gtactgaatg gtttgtccaa acagacagcc actgagattg ctgcaaaagc
     7021 aattatgggt tcttgtcaaa aattcctttc aagtgataaa aatccatggg aacttgtaga
     7081 ccaagtttct tctcctggcg gtacaactgt tgcaggtgta gttgccttag aagaagcagg
     7141 attcatccca gcaatcatca aaggaatcaa tacgacgatc gagaaagat
//
LOCUS       DACDYM010000100         6928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDYM010000100 DACDYM010000000
VERSION     DACDYM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.101"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1177)
                     /locus_tag="IWM59_002424"
     CDS             complement(<1..1177)
                     /locus_tag="IWM59_002424"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9980413.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     "
     gene            complement(1398..2858)
                     /locus_tag="IWM59_002425"
     CDS             complement(1398..2858)
                     /locus_tag="IWM59_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980414.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(3066..3992)
                     /gene="glsA"
                     /locus_tag="IWM59_002426"
     CDS             complement(3066..3992)
                     /gene="glsA"
                     /locus_tag="IWM59_002426"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9980415.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(4015..5442)
                     /locus_tag="IWM59_002427"
     CDS             complement(4015..5442)
                     /locus_tag="IWM59_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9980416.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(5473..6687)
                     /locus_tag="IWM59_002428"
     CDS             complement(5473..6687)
                     /locus_tag="IWM59_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9980417.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2133 a         1442 c         1166 g         2187 t
ORIGIN      
        1 ttcggtcact agctgagatc gttccgccac cgactaaaat aatcactgga ttctcgttga
       61 tcgctaaatc atgtgataat tgatcatatg cccgttgcaa gaaagtactt gattggaaga
      121 cgactggacg tactccctga gaagccaaag cagaagcgaa agtgatagag tgctgttcag
      181 cgatccccac gtcaaaataa tgatctggat gtttttcttg gaatgttttc agtccaaagg
      241 cgcctggtat cgctgcattg actgcggcaa taggagctgt acttgtttgc attttcttat
      301 ccaaataatc catgatgatc atattaaatg tttctccaga agttacttgc tttgcctccc
      361 cggtggacag atcaaatggt acatgccaat gatatttcat tttattatcg attgccggct
      421 gataaccgcg acctttttcc gtatggacgt gaagtacaat cggatgatca atatctttta
      481 cttcttcaaa taaacggatc accgtttcca gatcgtttcc attatccacg taacgataat
      541 caaatcccat tgctttaaat aggttattgc tgcttttacc gttggtttga cgaagctcag
      601 ctaagttttt atataaactg ccatgattct catcaataga catctcatta tcgtttacta
      661 atacgattag attggattgt aaaacaccac cattgttcaa tccttcaaaa gctaagccac
      721 cacttaggga gccgtcaccg ataaatgcta ccacatttcc tgattctttc ttcagatctc
      781 tcgctttagc taaacctgtt gctaagctga tagatgtaga tgtgtgcccc actgtaaaat
      841 agtcatgttt actttcatgt tggttggaat atccgctcac ttcacggtaa tgtccttttt
      901 caaaaccatt ttttcgacca gttaaaagct tatgaccata agactgatgg gaaacatccc
      961 aaacgatttt atcctttggt gaatcaaata cataatgaaa cgcaatcgtt aattctacta
     1021 cacctagatt cggaccaacg tgcccaccta catcgctgtc cttttgcaag attttttggc
     1081 gcatctcttc tgccaactgt ttcatttcct caacagataa ttttttgaca tctgcggggc
     1141 tattgattgc atccaataca tgtaatactg tactcataat acattctctc ctccgtacta
     1201 tcttctttta atgaaaatag atttgttaca ctttatctat tctactactt agagttgact
     1261 ctaagtcaat agcattttaa aaaaatttca gagaaagctt gttatataag tagatcttcg
     1321 cacgaatcag gactcaaata aaaaccttct acagacaatt aaaaagcttt gtagaaggtc
     1381 tcctaattaa ggtctcatca agaaatatgt ttttttaata ggtgaagttc ctgagcaata
     1441 tgatacaaat ggatgtttgc accttttaat gagattggat agatctttgt cttttcagtt
     1501 tctaatccat tttgatagct agcagcatcg cacaaaacca ctcttttttt cgcacataga
     1561 cattcatcag gcaattcttc taatgaacga aataaaatag gatttatttc agcgatgcct
     1621 tcttccgcat acaaatgaaa ttgggttcga taaaaaaatt gtttgactga actttctgaa
     1681 acaacaaaaa tttctatttc ctgtctcccc gcttgttcac tgttgatcaa agaaagactt
     1741 ttgacgaatt ttcgaatgaa attttcattc catcttagtt ggatttgatt tttctttttc
     1801 cactcttcca atatttctac aatttttccg tacagaagtt gttctttttc tgatagaaga
     1861 tagatccttt ccggtaaaaa tacttgtcca tctgcccaca acgtacggac aaattgtaaa
     1921 aaggattgct tgaaaataaa attttctgca tcgtcgattt cagcgatact aataatctgt
     1981 aaaatcaatt cattcagtga taggtcattg agaacaaaat ttttataaac aacttcgaaa
     2041 tcttttctta acagctccat gttctcattg gtataatttc tactgttgaa caaagaaaaa
     2101 atataataga catcttcttc ttgtaagtct agaattcctt tttgctcttc caatccttga
     2161 cagactaatt gaaataacgg cttcgtttct gcgcttttct tatcttcttc tttcagtcca
     2221 aggggaaatg cctttcttcc gattgcgatc tctattcctc gtatgataaa ggacttttca
     2281 tcttccgaat atcttctttt aaaaaaatga gtctctacat aatcaatcaa ctcgttacaa
     2341 gcttcttcta catacggatt tttattttca tagccttccc aattgacata acgtactaaa
     2401 gctagcaaga tgttccgcga gtcaaattct gggataatga tttctttatt tttcaaataa
     2461 tcattttctt taaaaaaatc caatacgatt tttttgattg tatatacttt gctcaaagaa
     2521 ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa
     2581 aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca
     2641 tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct
     2701 agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa
     2761 atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact
     2821 tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt
     2881 ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga
     2941 aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa
     3001 tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag
     3061 aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata
     3121 ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga
     3181 tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg
     3241 acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt
     3301 gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc
     3361 agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa
     3421 taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa
     3481 gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat
     3541 gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca
     3601 tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga
     3661 ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg
     3721 gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat
     3781 aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc
     3841 tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact
     3901 tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt
     3961 ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt
     4021 ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat
     4081 aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc
     4141 aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac
     4201 gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt
     4261 aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac
     4321 agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac
     4381 ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg
     4441 tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc
     4501 ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat
     4561 cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa
     4621 cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt
     4681 caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg
     4741 ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat
     4801 atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat
     4861 caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc
     4921 taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat
     4981 caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa
     5041 taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga
     5101 taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg
     5161 gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac
     5221 accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat
     5281 aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa
     5341 tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa
     5401 cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta
     5461 tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg
     5521 ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt
     5581 cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta
     5641 ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt
     5701 acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt
     5761 ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat
     5821 ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga
     5881 tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca
     5941 agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc
     6001 atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt
     6061 accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct
     6121 ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc
     6181 tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg
     6241 gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa
     6301 attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag
     6361 ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct
     6421 tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg
     6481 tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag
     6541 aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa
     6601 agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc
     6661 tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata
     6721 aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa
     6781 gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa
     6841 gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct ttttttattt
     6901 ttaaagatat tttttcataa ggaataaa
//
LOCUS       DACDYM010000101         6832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDYM010000101 DACDYM010000000
VERSION     DACDYM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.102"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..447
                     /locus_tag="IWM59_002429"
     CDS             <1..447
                     /locus_tag="IWM59_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980418.1"
                     /translation="AVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVHEDKV
                     KDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLPYQVA
                     DDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            469..699
                     /locus_tag="IWM59_002430"
     CDS             469..699
                     /locus_tag="IWM59_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980419.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            1245..1520
                     /locus_tag="IWM59_002431"
     CDS             1245..1520
                     /locus_tag="IWM59_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980420.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            1562..1984
                     /locus_tag="IWM59_002432"
     CDS             1562..1984
                     /locus_tag="IWM59_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980421.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            2003..3595
                     /locus_tag="IWM59_002433"
     CDS             2003..3595
                     /locus_tag="IWM59_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980422.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3607..3942
                     /locus_tag="IWM59_002434"
     CDS             3607..3942
                     /locus_tag="IWM59_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980423.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            4089..4283
                     /locus_tag="IWM59_002435"
     CDS             4089..4283
                     /locus_tag="IWM59_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980424.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            4315..6687
                     /locus_tag="IWM59_002436"
     CDS             4315..6687
                     /locus_tag="IWM59_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980425.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2764 a         1041 c         1365 g         1662 t
ORIGIN      
        1 gcagtgaagc taacgcatgt accagaagaa tatatcgcca tgcgtttaac cattattcct
       61 gaaaaaattg atccaaaagt ggatatgcaa gaaccacaag atctgattta ttacgtgcac
      121 gaagataagg ttaaagatca agtgaaagat gaagattatg aacaacacgc gatcaattac
      181 aaagtgaaag gatacaagaa agaacaaaaa gcagctcaaa aacaaatgga atctttccaa
      241 gcaaccattg atttgaatga agaattagtg aagaagctaa aagatcaatt gccttatcaa
      301 gtcgcagatg atcaggaaaa tactgaaaac aagattaata gctaccagca agaaattgaa
      361 acatcgaaaa cgcaactgaa agatcaagaa gagatcattc aaaaacttca agaaaaaatt
      421 gatcgtatga caaaagaaaa gatttagaag gacaggtgga gtgaaaaaat gaagcgaaca
      481 aacttggctg acaaagatac tttcgctgag cagaaagtat tgattaaagc ctatctcaaa
      541 gttgtaaaag ctaaagaaat atgtcgagaa gtgaatttat ctaacacaac aatatctgag
      601 ttgaagaatg gtcgaaagaa tttagatact ttaaaattca gatcatttga agctctatat
      661 gtagctgcta aaaaatattt tacagctgat aacaaataaa aaacaggagt attttatgtg
      721 aaaaaagtat ataaaaaatg cttatgaatc agctatatca ccttaaccat ttgttaatga
      781 gatgggagaa aacattaacc acgattctaa cgcaaacatc aagcacataa aacatttaac
      841 actcctcctt tctggctgaa gatttcaacc aaacaggaag acagcgtatc aattatagca
      901 tacacttttt cttaaattca tttttcatat gacaaaatat ttccttctaa tcgacaaagt
      961 tagaaggatt ttttattgtc taaatacaca aataacctta atttttaaag agcataagga
     1021 aacgtgatat cactttctca cgaaaggtgt taggtaggct gtgcctacca cgtttctaag
     1081 aaagataaat gatatcaaaa ttttggattt gtgtgtagat ttgtgggtag gatgaaagcg
     1141 aatgtgggca gtaaaataag cgtttgtgtg tagcaaaatc aaaaaatgtg tagggatatg
     1201 tgtagctaca catatcccta cacataataa atttggagga aaatatggac ttattaatta
     1261 gaaatattga agaaaagtat attaataaaa ttgacaaacg ttgtgaagaa cttacggtca
     1321 agactggcaa aaaatggagt cgaaatcaat acctaaaagt tttgatcgaa aatgattttg
     1381 atcacgctct tctgaattat aaaaaagacc aatttgatcg gttgttagaa aaatttgtgg
     1441 atattcaaac ttataatacg aaaactttag aagaatatat tgcaacaaat aatcaattaa
     1501 ttggtttgtt aattgaataa taaaaatagt attgtgaatt tataaaagga ggaaatcaat
     1561 tttggcaaaa gcaacaagaa aacatgtata tctttacgat atgcatatcg aagtacttga
     1621 tgaaataatt tctactcatg aagatatatc taattatagt gaagcagtca gacatttgtg
     1681 tatgatgtat gatccaaaaa tcgaagaaaa aataaatcca attcaaatta aattgaatgc
     1741 aatgggaaaa gaaatttcta ttttaaccga aatggtttca gagtttgcgg atcttcattt
     1801 gaaagataca ggaatactaa ttggtacaaa ttcaactgtt tatcaagaag cagaagaacg
     1861 tgtggaagga aaaattaaat ccaatcaaac caaaaaatat tcttctaagt taaagaaacc
     1921 agtgaatgaa tctgttcata ccagagtaga tccatttgat ccaacgaaga ttctaaaaag
     1981 ataaaagtag aaggagaacg tgatgaataa atcgcctgca gttattctta cttgtcaatt
     2041 taccttgccc aataaaaaat ctttttcaac atacattgat tatatgacaa gagaaaaagc
     2101 actggaagaa atggaacgac gtacgcccga agaagaacag gaactgcaac gaatcaagaa
     2161 tgctttagag gatttttata ttcctgaagg agaaactttt tcggaaagta agacggataa
     2221 agaattcagt gaaacgtcga atgaagctca atcgttgatg gaacatggaa tcaattttga
     2281 caagttagag gaacaagatt ttaccaaata tctttcttat atgacccgtc actatgcgtt
     2341 agaacaaaag aaagaactga cgcaaaggga acaaaaagaa taccaacggt tgaaccaagc
     2401 aatgaaaaaa tatcaaacag agccacataa aaaagcttca ggaaatcttc ctggagtttt
     2461 ttctatggct tcagacgaag tgaaaggaag tgatctgaag gagatcaaac aaatcttcca
     2521 aagaggacaa caaaaaggct caatcattta tcaagatgtt atctctcacg acaattcgta
     2581 tcttgaaaaa atgggcttgt acgatcccaa aacagatacc ttagatgaag aaggtttgaa
     2641 agcagctgga agaaaaatga tggagacatt attcgagaaa gaacaattga atgaaacagg
     2701 ttactggatg gcaacgattc accgtaacac taagcatatc catattcatt ttgcagttgt
     2761 tgaaaaagaa aatacgcgta aaccgtatac tgagattgaa aatggtgtga gttatatcgt
     2821 accgaagggg aagagaagac aagcaacatt agacgcaatg aaaaccaatt atgtacatga
     2881 gttagaacgt ttctcttatg aaaaggatcg aatgatcggt atggagaaac gaaatttatt
     2941 gacacgaaaa agtgatttgc gtaacacctt aacaaaagta gtaaaagaac cagcacgtta
     3001 cgatcgacac gccatgaact tattaaatga agtatataaa accctacctg aaaaacgttc
     3061 ggaatggaat tatggagatg aaaatcgaac caagctttca ccatttacaa ggggaaaact
     3121 tgatgagtta accaaatatg ttttaacaaa agaacctgga tatcaagaat atatacagat
     3181 ttcaaaagca ttgaaagaag aagccaaaag cctttatggg gaaagcaaaa gagaaacaaa
     3241 gaatgcagaa aaaaacgccc tctttgattt aaaaaagcgc acaggaaatg cgattttaac
     3301 agaactgaag agacaggata gtcaaataaa accattaata gaaagtgtca atcagatgca
     3361 atctatttac aatgaacgat cctttcgaac actcaaacag ttgccaacaa atgaatacaa
     3421 gtatagttat caagaattcg taaagcgtag gaaacaattc caacaaagag ttgtcacgca
     3481 gcgaagcttg cgtcaattaa atcgaacaat taatgcacat gaagaaaaat accacgcaag
     3541 taaagcatat gaagagcaac agcgaagaat ccaaatggaa caagaaagac aataggagga
     3601 agagaaatga aacaatattt agatcaatgg aaagtcatag aaggatcact aaaagaagaa
     3661 cgaatcgaac aacttcctga ttgtttagaa aaagagcatt tgtttcagat aagcgaaatg
     3721 ttgagaaacg aacagtttga tccaaatcaa tttttagttg ttgagtaccc agctactggt
     3781 gtgtattgtt gtaatcatgt gaagggtgaa aaatatttta ttattcaaga atacgaaggt
     3841 aagttagcgc cttattatac tacttgggaa ataaatgaag agggaatcaa taatttccca
     3901 tgtgaaagca ttgaagagtc tatttctctc acggaatgtt aaataaatag agacgtaaaa
     3961 gagtcctgaa cgtgattgtc caggactctt tttcaatgaa ttgttttccg ccaattcact
     4021 gagtggtgcg attcaatttt attatatcaa aagaaagtga gattccaaag tgaagggagg
     4081 aagaagaaat gactcagaca acgattggtg ttgtggttga aaaaagcagg gataatctcg
     4141 tttttgtgac agaaattcaa acaggtagag cttttgtagt cacagacaaa gctgcaaaag
     4201 cgtatcaaaa tggcaatatt ttaacgttaa atctgactac gaagatgttc gttgatgcag
     4261 tagaaaatta tccatttgtt tagacaaacg atattttaca aggaggaaac ggacatggca
     4321 gaagaaaaag aaccactaga taatagtcgt ttgggaaagt ccaaaagaaa attagtacgg
     4381 ttgcaaaacg aattaaataa tcaatttcaa aagctatttg atcaccaaaa attgacaaat
     4441 gggcaaccaa tgaatgacaa acgaaatggt cgttcatggt ttaaccagca agaaaaaata
     4501 gaaaataaaa tacaaagttt gagagaagag atcaagcaac aagaaaaaca ggtagaaaaa
     4561 cttgaaagac aagaagagct aaaggaaatg ggttataaca agtacggtgg cctggacatg
     4621 acgatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa aggtgaatcg
     4681 actttttcag ctgccacgat ccgaaaatac caacgaaaat tggaaacatt agaacagctg
     4741 aaagaaaaat cagagaaagg aaaagaaaat cttcttcctg aagtacaagc gatcattgac
     4801 agcggacgag tgacgcaatg gaaaaagaat ccaactatct attttctgaa aggctatcga
     4861 aaagtcgctt tagaactcaa tgaaaacggg ggatttgagg aatctaaaaa ataccaagcc
     4921 acgtcggaag aagaaaaggc agctgttcgt gaattacttg aagaagctac acctactgat
     4981 tctgtgaagg agaaagaaac cgatcatttc tatcgagtag aattcaacga aaatgatcca
     5041 gcaaaagggt tgaaaagtta tggtggggag atcgtaacac tagaattgat cgatcaactc
     5101 aaaacactgg ataaccaata cgccgcagaa gcaggctatt ataaattcta ttttgaagaa
     5161 gtgaagaatg gagaagtagt cgatcagata cgaatggatc taggggatgg cgtcaatagt
     5221 aatcacccca tctatttaga gttagctaga ttgtgcgaac aatcacctga aaaggaacaa
     5281 gaacaaacag tcaaaaatga acctatttca aaagtacaaa acgagattaa agagacaaaa
     5341 acaaaacaaa agggttatca aaaacgaacg gcagaagaga ttaagcaaga agtcaaatca
     5401 ctttcccaac aggcgcttga tgcggtgaaa aagcatactc attcacctga agacgtcaaa
     5461 gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca attattgatc
     5521 gaggaacaat ttccaggcgc aacggcttgt ttaggtcgtg ccgcgttgaa aaaagaagga
     5581 atctatatca aaaaaggaga acaagggaac aagatctttg tcagaaaaac ggtcaaagga
     5641 ttttatgaga aaggacgtgg ctttgttcga gaaacagagg ctacaccaga agataaaaaa
     5701 cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat tgaaaaagtg
     5761 tttgatatca cacaaacaca attgaaacct gaagactatc cgaaaatctt cccgaaccgc
     5821 gtctttgatt ttcaattgga tcaaacaggg cagacagaac ttcaagttgg gattcaagcg
     5881 gtagccaaaa atattggaat tcaaattcga gatatgacag aaagtgaagt ctatcaaaga
     5941 gaattaggac aagcacgtgg ggcgtacgtt cgtaacgaat ttactggcga agaagaaatc
     6001 gtcatgaata ctcgaaatac tcctacgcag catttagcca caagtattca cgaattagcc
     6061 catgctagaa tgcacaaatt ttcagaatta gatactgcaa caaaagaact acaggcagaa
     6121 atgacctcct atattgtgtg caagcatttt gggatggaca cctcggagaa agctattcct
     6181 tatattgcga catggacgaa gaatagtcag acgttggatg ataaggaagc tgcggaacgt
     6241 gggaaaatca tgaatgatgt cagtcgtgtc gcaaatgaat tcattcaaac catttctaat
     6301 gaaatcaatc aatacagaga aagagaacca gagatccagc cagcagaagt gaagcaggag
     6361 cataaaaata ctgtagaaac ggcagaacaa aagaatattg tgcccgttgg ctcactttgg
     6421 atcgacaaga aagacgggaa agtcctggaa gaagacacgg gccgaacgtt acatttgaag
     6481 gatgcggcgt ttattgaaga tccgcagcat aatcaagtga ttttaaaagg aaaaagttat
     6541 ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa tccattccga
     6601 ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta ttatgaacaa
     6661 caattgtcac aaaaagaacg cgtataaaac aagcaagaaa gactatctga aaaatggata
     6721 gtcttttttc ttttgggagg tttgaaaatg acagagaaaa aaatgagtct cattgatcga
     6781 tgcaagcaaa tggatattgt ggattttgca cgaaacaatg gtttagcggt gg
//
LOCUS       DACDYM010000102         6676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDYM010000102 DACDYM010000000
VERSION     DACDYM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.103"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..172
                     /locus_tag="IWM59_002437"
     CDS             <1..172
                     /locus_tag="IWM59_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="modulates transcription in response to the
                     NADH/NAD(+) redox state; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9980426.1"
                     /translation="SQSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTL
                     IYFDENYSEVFS"
     gene            565..3210
                     /locus_tag="IWM59_002438"
     CDS             565..3210
                     /locus_tag="IWM59_002438"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9980427.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3520..4833
                     /locus_tag="IWM59_002439"
     CDS             3520..4833
                     /locus_tag="IWM59_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9980428.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            4823..5476
                     /locus_tag="IWM59_002440"
     CDS             4823..5476
                     /locus_tag="IWM59_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9980429.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            5531..6223
                     /locus_tag="IWM59_002441"
     CDS             5531..6223
                     /locus_tag="IWM59_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP9980430.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6373..6573
                     /locus_tag="IWM59_002442"
     CDS             6373..6573
                     /locus_tag="IWM59_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9980431.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2193 a         1120 c         1486 g         1877 t
ORIGIN      
        1 ttcacaatct gctattgatg aaattgtcaa aggaaatgta accgctatac ttaattttgc
       61 gcctgacaga gtaactgtcc cagcttatgt caatgttcaa tacattgatt tgacaaccga
      121 gttgcagacg cttatttact ttgatgagaa ttactcagaa gttttttctt aggaaaattt
      181 gagtaggctt attgatcttg cgagaactcg tcgtaattct ttcaaaaatt ttttagtact
      241 aaatgaagtg gttttttcac gaccacttgt tgactaaaaa aaaaaaccat tgtagaatga
      301 agcataacgc tgaacaagag gagtaactgg cacattcatt cttagagaga gaactatctg
      361 ctgaaaggtt ctcaatgaca accagtgaag gtagcttggg agtttctgtc ttgaatggag
      421 tagagataga cgagtcgtga tcgttaactc acttgagagg caataatgat tgattattgc
      481 aatttaggtg gtaccgcgta aatcatacgt cctagaatac gaaagtattt taggacgttt
      541 tttagtttaa aggaggaaaa cagcatgacg gaagaaaata acctatcaac aaaatataat
      601 ccgcaagaag ttgaagctgg acgttatcag cattggttag aacaagattt gtttaaaccg
      661 agcggagata aaaaagcaaa accttattca atcgtaatcc cgcccccaaa tgttactgga
      721 aaacttcatt tagggcatgc ttgggatacg accttgcaag atatgctgat tcgtcaaaag
      781 agaatgcaag gatatgatac tttatggctg cctggtatgg accatgcagg aatcgcgaca
      841 caagcaaaag tagaagcgaa gcttagagag caagggattt ctcgttatga tctaggtcgt
      901 gaaaagttca tcgatcaagt gtgggaatgg aaagaagagt atgcgagcca catccgcgaa
      961 cagtgggcta aaatgggctt atctttagat tacagtaggg aacgttttac attagatgat
     1021 ggattatctg aagcagtacg taaagttttt gttaccttat acgaaaaagg tttgatttat
     1081 cgtggtgaat atatcatcaa ctgggatcct caagcacgaa cagctctatc tgatatcgaa
     1141 gttattcata aagatatcga aggcgccttt tatcatatga gctatgaact agcagatgga
     1201 tcaggcgtag tagaaattgc gacaacccgt ccagaaacga tgttgggaga tacggcgatt
     1261 gctgttcatc cagaagacga gcgttatcaa gcgctcatcg gcaaaaaagt gattctacct
     1321 ttagtcaata aacaaatccc aatcattgca gatgaatatg tagatatgga attcgggaca
     1381 ggtgtagtaa aaatcactcc agcacatgat ccaaacgact ttgaggtagg taatcgtcat
     1441 gatcttccac gcgtaaatgt tatggatgat gatggtacga tgaatgaact agcaggaaaa
     1501 tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg tgcaagatct gaaagaaatc
     1561 ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg gtcattccga gcggacaggt
     1621 gtcgttgttg aacctcggct gtctactcaa tggtttgtaa aaatggcgcc gttagcgaaa
     1681 aaagccattg ataaccaaga aacagatcaa gcagtggaat tcttccctcc aagattcaat
     1741 caaacgtttt tacgttggat ggaaaatgtc catgattggg taatctcacg acaattatgg
     1801 tggggacatc aaattcctgc atggtatcac aaagaaacag gtgaaatgta tgtagggatg
     1861 gaagcaccag cggatagtga aaactgggta caagatcctg atgttttaga cacttggttt
     1921 agttcagctt tgtggccttt ctcaacaatg ggctggccag atacggaaag cgaagattat
     1981 aagcgatact tccctactag cactttagta accggttatg acatcatttt cttctgggta
     2041 agcagaatga ttttccaaag cttggaattt actggcgaac gtccatttga gaatgtcttg
     2101 attcatggat tgattcgtga cgaacaaggg cggaagatga gtaaatcatt aggtaatgga
     2161 atcgatccga tggaagtaat cgaaaagtat ggagcagatg ctctccgctg gttcttatcg
     2221 aatggttctg caccaggaca agatgtccgc ttcagctatg aaaaaatgga tgcttcttgg
     2281 aactttatca acaaaatctg gaatgcttca cgtttcgtta tcatgaatat cgaaggaatg
     2341 accgtagaag agatcgattt atctggagaa aaatcagtgg ctgaccggtg gatccttact
     2401 cgtttgaatg aaacgatcga acgagtaact gagctatttg atcgttttga atttggtgaa
     2461 gcaggtcgac aactgtataa ctttatctgg gatgattttt gtgactggta catcgaaatg
     2521 agtaaagaag tactttatgg agatgatgct gtttcaaaac aaaccacccg tagcatctta
     2581 gtccatacac ttgatcagat cctacgttta ttgcatccaa tcatgccatt cgttacagaa
     2641 gaaatatggg aacatattcc tcatcaggga aattctctag tagtggcaga atatcctgtt
     2701 gtacgtccag agtttgatga tgaaacagct tctaaaggga tggaagtatt gaaagaattg
     2761 attcgagccg ttcgaaatat tcgttcagaa gtgaatacac cattgtctaa accaattacg
     2821 ttgctaatta aaacaaacga tgctaaaatc gatcaatttt tagttgaaaa tacaagctat
     2881 atcgaacgct tctgtaatcc cgaagagttg acgatatcaa gtgagatcac tgcgccagat
     2941 ctagcgatgt cagctgttct gacaggtgca gaaatcttct tgccgttagc aggattgatc
     3001 aatattgaag aagaaatcaa acgattagaa aaagaactgg aaaagtggac aagcgaagta
     3061 aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt ctaacgcgcc ggaagaagta
     3121 gtagaggcag agcgtgcaaa agaaaaagat tatttagaga agcaaacggc tgtcaaagaa
     3181 cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa agagagtgtg aaatcagcta
     3241 gctattacta aaagctagct gattgaacat cgttacagga aagagtttga gcctcactct
     3301 tagcttcgat tcataaggag atgtgacata aattttgtcg catctctttt ttagtctact
     3361 cttattatgt aggagaagga gggaggtgga tcatgtatca agagtcaata aagtagttga
     3421 taaaatcgtt cctctctctg tttcttttct ctttgtcata cttttatttt tcttatgtgt
     3481 tctatttgtt ataataaaag agaaatgggg gaattgttga tgacgattga agaagcaata
     3541 gaatggatcc atagtcgatt accatttggt tcacgtcctg gccttgaccg agtcgaagct
     3601 ttattagaag tggtagggca tccagagaga aaagtaaaaa cgatccatat cgctggaaca
     3661 aacggaaaag gttctactgt agcttatttg agaagcctat tagaagaggc tgggctgaca
     3721 gtaggtacat ttacttcacc ttatatcgaa acgttcaatg aacggatcgc aatcaatggc
     3781 aaaagcattc cagacaatga tttgatccag cttgtaaaaa aatatcagcc actgatcaaa
     3841 caacttgatc aatttgaagc agtagctggt attactgaat tcgagacatt aacagcaatg
     3901 gcatttgatt attttttgat gaagaaagtg gacgtagcga ttatcgaagt tggcttaggc
     3961 ggcttattgg attctaccaa tgtagctgta ccaatgctga cgggaataac aacgattgga
     4021 cttgatcata ccgaaatact tggagataca attgaagaaa tcgctgcaca aaaagctgga
     4081 atcatcaaac aaggcgttcc agtagtaaca ggaaatattg ccccagaagc ttttactgtc
     4141 atccaacaaa cagctaaagc aaaaaatgca ccgctttatg tatacgggga aaattataaa
     4201 ataaactatc tgcatcctga tgagatgtgg ggagaagtat ttgaattttt taatgaaata
     4261 ggaaagatcc ctcaattaac cactccgctt ttagggcaac atcaagctga aaatgcagga
     4321 gtagccatcg aattgtattt tgtttactgt caattggaaa agctgccttt ccgagaaaaa
     4381 gatgttttga agggattgaa gaaatctcga tggccaggaa gaatggagcg tttgagtaac
     4441 gagccattag ttgttttaga tggtgcccat aatacgcatg caatgaaccg gcttgttgag
     4501 aatttgaaaa aagaattcaa aggatatcaa atcaacattt tgttttccgc cttacgaact
     4561 aaaaatgtta gagaaatgct agaacgattg cagcaagtac caaatgtcca tatttatttg
     4621 acgacctttg aacatccaaa agccatcgaa ttaagtgaag tatcagattt agcaagtgag
     4681 agagtatcaa tcgtttcatt gtggcaattc ggattagcag atatattgga aaagatgaca
     4741 aatgaagacg tattagttgt gaccggttca ctttatttca tttcagaagt tcgccattta
     4801 ttaatacaat taggaggaga tcatgaagta taatgcagtg atttttgaca tggatggttt
     4861 gctgttcgac acagagttag tgtattacga agccacacaa gtagtagcag acaagatggg
     4921 ttttccttat gataaagacc tgtatttaaa attcttaggt gtttcagatg aagaagtatg
     4981 ggcgaattat catatgattt tctcggaatt tggcaaaaca agtgtacaaa gattcataga
     5041 tgattcatac caggaaacgc ttgaacgttt ttcgcaagga gcggtacaaa tgaagcccgg
     5101 tgtgttggaa ctcctctctt ttttagaaga acatcagatt cccaaagttg tcgcttccag
     5161 taaccaacga gcagtcattg aacttttatt gaagaagaac aatctgtttg aacgatttga
     5221 gacgattgtt tctgcagaaa atgttcaacg agcaaaacca gacccagaaa ttttcttgtt
     5281 agctaatcag aagctaggta cagcgaagaa agaaacattg attttagaag attcacaaaa
     5341 tggtatttta gcagcttctg cggctgagat cccggtagtc atggtaccag atctgcttcc
     5401 accatcccaa gaattgatca aaaaaacagc tggggttgtt tcttctttac atgaaatccc
     5461 tgcattgtta aaataaaaat ccatctcttt tcgtatctta ttttagataa gatagaaagg
     5521 agatgttttt ttgggaaaac gattgatcaa agaagtacct actagctctt taccaaggga
     5581 acgtatggaa atttatggcg cggaagcttt atcggatcag gagcttttgg caatcttact
     5641 gcgtaccggt caacaccctt atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt
     5701 tggaggactg gcctctttac gacaggctac tttgcatgag ttagaagaga ttcgagggat
     5761 aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac
     5821 ggatcatgaa agagcggctc caccggttcg taccagctac gaattggccc agcaattgat
     5881 tctggaaatg aaagatcaca aacaagaaca tttggtttgt atttatttgg acacgaagaa
     5941 tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg aatcagtcag tcgctcatcc
     6001 tagagaaatt ttccattatg ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa
     6061 tcatccttct ggtaatgtta ttccatcgca gcaggatatg aattttacca aaaggataca
     6121 aaagtgtgga gaaatgatgg ggatcactgt actggaccac ttgatcatcg gacgcaaacg
     6181 atattttagt ttgagagaag aaggaatgat ggaagagaag taagttgctt tcttgcaaaa
     6241 aaaatgaagc aagtgtacaa ttatattgta attttgtttg ggaatagaag ttgaaacatc
     6301 tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa attagtgctt ttggcacgag
     6361 gaggatatta ttatggaaaa cggaacagta aaatggttca acgctgaaaa aggatttgga
     6421 tttatctcac gtgaagatgg aagcgacgta ttcgttcact tctcagctat ccaaggtgac
     6481 ggattcaaaa cattagaaga aggtcaagca gtgactttcg acgttgaaga atcagaccgc
     6541 ggcccacaag ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa attgatggta
     6601 gacatcaatt tttacgagag aacaaagcga gtaacaaaaa tacctgccac aagagacaat
     6661 ttctcttgtg gcaggt
//
LOCUS       DACDYM010000103         6375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDYM010000103 DACDYM010000000
VERSION     DACDYM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.104"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            37..1059
                     /locus_tag="IWM59_002443"
     CDS             37..1059
                     /locus_tag="IWM59_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9980432.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPREYKVIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEVISLKDICREVAKELKDCERSNGTYDFDRFYQGKGD
                     INSQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1070..3274
                     /gene="topB"
                     /locus_tag="IWM59_002444"
     CDS             1070..3274
                     /gene="topB"
                     /locus_tag="IWM59_002444"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9980433.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            3520..4113
                     /locus_tag="IWM59_002445"
     CDS             3520..4113
                     /locus_tag="IWM59_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9980434.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            4129..5028
                     /locus_tag="IWM59_002446"
     CDS             4129..5028
                     /locus_tag="IWM59_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9980435.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            5031..5516
                     /gene="ssb"
                     /locus_tag="IWM59_002447"
     CDS             5031..5516
                     /gene="ssb"
                     /locus_tag="IWM59_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9980436.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5882..6142
                     /locus_tag="IWM59_002448"
     CDS             5882..6142
                     /locus_tag="IWM59_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980437.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2314 a         1105 c         1313 g         1643 t
ORIGIN      
        1 cccgattggt tgattgatta tgcagaaaaa gaaggattga ttgccgaact aaagccaaaa
       61 catgaacggc aaaatttctt agttcgagac gatcgtttgg atcatgcggt cgccttttta
      121 tggaaagatc cacagacaaa agagacagta ggggcgagtt atcaaggaac gttcattgat
      181 tatgaacgtt ttggtgagag aggcacatat aagcatatag ataaaaattc gacagcgaat
      241 catggcttta acctaaagat tggtgatcct aaacagttaa aattttttga gagtagtatt
      301 gatttactta gctacgctgc actgaatcgt gatcagttaa acgatacgtg gttagtctct
      361 atggaagggt taaaacacca tgtgatcagt cattattttg gtgaggcagt atctgaattg
      421 agaaagaaac aagcctttcc tcaatccatc gagatttgtg tggataatga ccgtgcagga
      481 catattttct acgaaaaaga acaattaatg ggcgcagttg atccatttac gaatcagaaa
      541 gtacgttgtg aacgaggaat cgctaatgat tggcaagtgc caagagaata caaagtcatt
      601 tatgaagaag tcgcaaaaga agaaaaggtc acaccagaag ctattatggc catacacaag
      661 acagaaaaca atctgcaatt aacgaaccaa ctggtttccg ctcacaaagt caatgcgtct
      721 tttggtcagc agctttctgt taacgattca atagaagtca ttagtttgaa agatatttgt
      781 agagaagtcg caaaagaatt aaaagattgt gaacgctcga atggtacgta tgattttgat
      841 cgtttttatc aaggaaaagg ggatattaat tctcagattc ttttctctta taaagcagaa
      901 caatactaca aaggctataa aaatcatgaa catgaatttg tacctgaagt gaaaaaggat
      961 tggaacgatc aattgaagca tgagatccat caacaggaaa ttagaaagca aaaacgtgcg
     1021 atgttgtttc aacaaggcag acaacaagaa agggagtgag atcgtcacga tgaaaactgt
     1081 cattttagca gaaaaaccgt cacaagcgaa ggcctatgcc gatagctttt ctaaagccac
     1141 ccgaaaagac gggtattttg aaatccaaga tcgattgttt tcgggtgaaa ctgtgatcac
     1201 gtatggtttt ggtcatttag tcgagttaga ttcgcccgat atgtacgatg aaaattggaa
     1261 acaatggtca ttagagcatt tgcctatttt ccctacacat tatcactatc atgttcctaa
     1321 agataagaaa aagcaattca acgtggtgaa acagcaactt caatcggccg acaccattat
     1381 tattgcgaca gatagcgatc gtgaagggga actgatcgct tggacgatca ttcaacaagc
     1441 aggcgctgat caaggaaaaa cctttaaaag actttggatc aactccttag aaaaagaagc
     1501 catttatcag ggattccagc aattacgaga cgcagaagag acctatccca aatttgaaga
     1561 agcacaagca cgccagatcg ctgattggtt gatcggcatg aacggcagcc cactgtatag
     1621 tttactcttg cagcaaaagg gcataccagg aagtttttcc ttaggcagag tccaaacccc
     1681 aacgctttac atgatttatc agctgcagga aaagattaga aacttccaaa aagagcctta
     1741 ttttgaaggg aaagcccaag taaccgctca aaatggcata tttgatgcga agcttgatcc
     1801 caatgaaacc caagcgacgc aagaagcatt tgaagcctat ttaaaagaaa aaggtgttca
     1861 agtaggaaag cagccaggca cgattcttga agtcgaaacc gagaaaaaaa gcgcggcaag
     1921 tccacgtctt ttttctctat ccagccttca gtctaaaatg aatcagctca tgaaagctag
     1981 tgccaaagat actttggaag ccatgcaagg gttgtatgaa gggaaatatc taagctatcc
     2041 acgaacagac accccttata ttacagaagg agagcatgcg tatctgttgg atcatttaga
     2101 tgaatacaag cattttttaa aggctgaatc attccctact cctattcata caccgaatag
     2161 tcggtatgtg aataataaga aagtccagga acattacgcg atcattccta caaaaacagt
     2221 tatgacagaa gcaacgtatg aaaagctgac acctttacaa caagcgattt atgatcaagt
     2281 tttaaagacg acggtggcca tgtttgcgga aaaatatacc tatgaagaaa ctacgatttt
     2341 gacacaagtc cagcagcttc agctaaaggc agtcggaaaa gtaccagtag atctaggttg
     2401 gaaaaagcta tttggcaaag agagcgacag aaaagagaag gaagaagaac cgctacttcc
     2461 taaagtaaca aaaggagaaa cggtgacggt agatctacaa atctttgaaa aagaaaccaa
     2521 gccaccacaa ccttacacgg aaggtacact aatcaccgcc atgaagacag ctggtaaaac
     2581 cgtggatagt gaagaagcac aatccatatt gaaagaagtg gagggaattg gtacagaagc
     2641 gacacgagca aatattattg aaaccttgaa gcaaaaggaa tatatcaagg tagagaaaaa
     2701 taaacttgtg gtgacgaata aaggaatttt gctttgtcag gctgttgaaa aagaaccgct
     2761 gttaacaagt gccgaaatga ccgccaaatg ggaaagctat cttacaaaga ttggtgagca
     2821 aaaagggaca caagctactt ttctagcaaa tatccaaaaa tttgtttccc acttgttgga
     2881 agtcgtccca ggacaaatcc aagccactga ttttggagca acgttacaag aggtgaaagt
     2941 agccagtgaa aagcaagaag cggcgcgtca tttaggatct tgtccaaagt gccaggaaaa
     3001 agaagtcata ctttatcaga aggtggcgac ttgcacgtct gaagcctgtg attttaaatt
     3061 gtgggtaacg attgccaaaa agaaactcac agcgacacaa ctgaaagaga tcattcagaa
     3121 cggccgaaca agtcaaccag tcagaggact aaaaggtcag aaaggttcgt ttgaagcaac
     3181 gatcgtttta aaagaagact ttacaacagg atttgagttt tctgaaaaga agaagaccac
     3241 ctacaaaaga aaagtgagac gtaaaacgag ttagaattga cggagggaaa aacgatgaga
     3301 aatacaacgc ataaagaaca agaaatcgaa aatcaaaaag ccaaaatacg ttcagagcag
     3361 gagaaaatgc gtgccagcaa acgaagacta cagcaattga agaatcaaca acggattcaa
     3421 aaagaacttg aattagatcg ataaatcctc ctgttgttaa tattaaacta acatgttata
     3481 gttatgttag tttaatatta acattcgaga gggaaagcga tgaaagataa agtcaagtgc
     3541 atttttgaga aatacaatgg taatctatcc ttgaaggatg ctagaaatga agggttatca
     3601 cctatgacgt tagatcgttt agccgaaaag gacaaaatag aaaaaatagc atcaggtttt
     3661 tataccttaa aagatagttt tgtggatgaa ttatatctcg ctcaaagtat ctatactaga
     3721 ggaatcttct ctcacgagac agcgcttgat ctttttcaag taggaacata tatccctaaa
     3781 aaaattaata tgatgtttcc taaaggatac aatgtttcga aagagaattt agatcagtat
     3841 gccattcaac cgcattacac gaataaagaa aactttgaac tgggaatcac agaggcagaa
     3901 agcttttacg gcaataaaat tttcgtctat gatttagagc gaacgttatg tgatatgtgg
     3961 aatcccagat ataaagcaag cgtagaagta aaacaagaag cattaaaaga atatatgaca
     4021 agtaccagca gaaatacaaa taagctaaga agatacatga atattttaaa atcaccaaaa
     4081 gaaatgactt tgtatatgtt gccactttat tgagaggagt aaaggtgaat gacaccgaca
     4141 caatttaaag cgaaaacaag aaatgttgca gctgagaaac atttaaaccc acaaattgtt
     4201 caaagatatt atatgatgga aaaattatta gaatgtattg ttgaatcgcc ttatcatgat
     4261 gattttgtat taaaaggggg atttctgatc ggatcaaaat acgggttaga aaaccgttca
     4321 accgttgata ttgatacgac ttatcgaaat tcgaaactaa caaaagaaaa attagaaact
     4381 gttttttctg aattaacagc tgctcctaca aaagatggca ttcggtttga attaaagggc
     4441 ttaacagaga cgagggaagc cgattattac ccaggttttc aagcaaagtt tattgctttt
     4501 cttgaaaatg cacgtgtacc ttttaaatta gatattactt caggtgattc tattattcca
     4561 gcagcattga tatatgaaca taaattgatg tttgaagata aaacaattca tattccagct
     4621 tatcctacgg aacaaatttt agctgaaaaa ttacatgcca cgtttagttt cggaatagat
     4681 aattcacgta tgaaagattt ctatgatctg tatatgatcc ctaaactaga acgtatcgat
     4741 gagataaatt tgtataagag tgttcaagga acatttaagg aaagaaataa tcaatcagtg
     4801 tttaagaatt tgtataatca ggaaatgcct caattgatga agaatgaggg attgagggag
     4861 aaatggaaaa gatatcagca agaaaactat tttgccaaag atatttcttt cgaatctacc
     4921 ttaacttctg ttgattcgct gatgcaatca attgtcgagc aagaggaagt agaacgaaaa
     4981 caaaatttta agagatcaca acaaatgaat caagaaatgg agagataaag atgatcaata
     5041 atgtaacttt agtaggtcga ttaaccaaag atcccgattt acgttacacc gcaagtggca
     5101 ctgcagtgac cacttttacc ttagccgtta accgtaattt tactaatcaa agtggcaatc
     5161 gagaagctga ctttatcaat tgtgtgattt ggcgcaaacc agctgaaacg atagcgacat
     5221 tagctaaaaa aggcagcttg atcggcgtcg tcggacggat tcaaacgcgg acttatgaca
     5281 accagcaagg acagcgtgtc tatgtgactg aagttgtggc cgataatttc caactattag
     5341 aaagtaaagc agcgacagaa agccgtatgc acgctgatca atcgtccagc agtccaagcc
     5401 ctaccacctt tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac
     5461 agaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttctaagtag
     5521 cattttctat tggttgcatt tgctacggtt tatgttataa tttagacaac aaaaagaatt
     5581 ttcaatctat aaccaaaaac cactagctgg cgggctagtg gctttctttt acgtttctat
     5641 tataagaatt aacgggtagg cttactgtct ttcttggcaa atttacaatc aagccatttg
     5701 cagatatagt gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa aagaattttc
     5761 aaacctataa cctcctcccc ttgccagatt aggccggggt agcagacagc aagactatta
     5821 tagcataatt gaaatatcca aatgattgga tttttgctat aattttagcg aggtgattgt
     5881 gatgaacaaa tatgttggtt tgcttgataa aatccgagta ataaaaacgc aaccgttatt
     5941 ggttcgcttt actttacaaa cgattcatga atcgattaat tgtgtagttg cagatattga
     6001 aattatcgat aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca
     6061 cttcaataaa cgaaatcagc tagttattgc tagcatgtat gttagaaatc ccgatcattt
     6121 tacgcgatcg atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc
     6181 tctttttgtt ttacatagaa acaggaaaag acctttttag gaagacacaa gtgccttctc
     6241 aattgaaaaa cctttctctc actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa
     6301 cgctgcacgt tcactaagat atggtgtctt gcaaagcaag acaaaataga caaaacttcc
     6361 tctcaccaaa aggtt
//
LOCUS       DACDYM010000104         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDYM010000104 DACDYM010000000
VERSION     DACDYM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.105"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IWM59_002449"
     CDS             334..714
                     /locus_tag="IWM59_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9980438.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IWM59_002450"
     CDS             696..1610
                     /locus_tag="IWM59_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9980439.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1570..1848
                     /locus_tag="IWM59_002451"
     CDS             1570..1848
                     /locus_tag="IWM59_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980440.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDS
                     QVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
     gene            1855..2544
                     /locus_tag="IWM59_002452"
     CDS             1855..2544
                     /locus_tag="IWM59_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980441.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IWM59_002453"
     CDS             complement(2665..2751)
                     /locus_tag="IWM59_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9980442.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IWM59_002454"
     CDS             3427..4377
                     /locus_tag="IWM59_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9980443.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IWM59_002455"
     CDS             4398..4931
                     /locus_tag="IWM59_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9980444.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="IWM59_002456"
     CDS             5567..6100
                     /locus_tag="IWM59_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980445.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1101 c         1173 g         1706 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggtt tagtatacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DACDYM010000105         6192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDYM010000105 DACDYM010000000
VERSION     DACDYM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.107"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IWM59_002457"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IWM59_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9980446.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IWM59_002458"
     CDS             complement(2203..4389)
                     /locus_tag="IWM59_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9980447.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IWM59_002459"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IWM59_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9980448.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IWM59_002460"
     CDS             complement(4611..5051)
                     /locus_tag="IWM59_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9980449.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IWM59_002461"
     CDS             complement(5125..5664)
                     /locus_tag="IWM59_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP9980450.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1607 a         1393 c         1090 g         2102 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aa
//
LOCUS       DACDYM010000106         6069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDYM010000106 DACDYM010000000
VERSION     DACDYM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.110"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..134
                     /locus_tag="IWM59_002462"
     CDS             <1..134
                     /locus_tag="IWM59_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9980451.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(220..1314)
                     /locus_tag="IWM59_002463"
     CDS             complement(220..1314)
                     /locus_tag="IWM59_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9980452.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1503..2264
                     /locus_tag="IWM59_002464"
     CDS             1503..2264
                     /locus_tag="IWM59_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9980453.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="IWM59_002465"
     CDS             complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="IWM59_002465"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9980454.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3039..3794
                     /gene="fabI"
                     /locus_tag="IWM59_002466"
     CDS             3039..3794
                     /gene="fabI"
                     /locus_tag="IWM59_002466"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9980455.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3904..4917)
                     /locus_tag="IWM59_002467"
     CDS             complement(3904..4917)
                     /locus_tag="IWM59_002467"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9980456.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5082..6002
                     /locus_tag="IWM59_002468"
     CDS             5082..6002
                     /locus_tag="IWM59_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9980457.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1863 a         1205 c         1081 g         1920 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact
      181 ggctatttct agcggattat ttctgcaata actaattatc tattttttat tttcaattaa
      241 tttaaagctt gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct
      301 tcctacaaca ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc
      361 tttgtattga ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt
      421 attatgaggc gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat
      481 atattctgag cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt
      541 ttctgtaata ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc
      601 tgccatttct ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat
      661 caatcctgta aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc
      721 cagcgtgcac aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac
      781 gaatgccatc gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc
      841 gtatcctaaa ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt
      901 gtttggcgaa taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc
      961 gattttgata ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat
     1021 atctaagcgg accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat
     1081 ctccaactgt tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt
     1141 cgcgtaatca actacttctt taaattctga aagaactttt tctttatcat cattgatctg
     1201 cagcaaactg gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt
     1261 gtccttttca aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc
     1321 atttcctcct gcatcttttc gtttttgtat ccacttacat tatagcaaac acaaagtaaa
     1381 tggtctacac cttttctttt tcttttaaaa taaatgataa attccaataa tttaagtagg
     1441 tattttccct ctattttgtt atcattaaag ataacaaaat agtaaattgg aaaggaacag
     1501 ttatgaagaa aaataaagtt attttaagta ttagtttagc agcagcattg ctgtttgctg
     1561 ctggttgttc tgcaaacaac acgacttctt ctagttcgtc tacagaaagt tcaaaaagta
     1621 catctactgc ggaaagcacg actgccagta gcgtcgattt gaacgcattg gaattaccac
     1681 aattgacgaa agatgtcgcc gaaaatgaag atctggtcga aatggtgacg aataaaggca
     1741 caatcaaaat caaattattt cccgaacacg caccaaaagc ggttgaaaac tttatgacac
     1801 atgcaaaaga cggttactac aacggactga ctttccatcg cgtcatcaaa gatttcatga
     1861 tccaaggtgg cgatcctaaa ggcgatggca ctggagggga aagcatttgg ggagaaggat
     1921 ttgaaacaga gatcaacaac cacctttata atataagagg tgcgttatct atggctcgct
     1981 cacaagatcc taacagtaac ggcagccagt tctttatcgt tcaaaataca gatgatatgc
     2041 atgatggtct tttaaaagac gactatcctc aagcaatcat cgacgcttat aaaaacggcg
     2101 gttatccttc actagatggc cattatactg tcttcggcca agtgattgaa ggaatggatg
     2161 tcgtggatgc tattgcaaat atcgaaacag atagttcaga caaaccaaaa gaagaagtaa
     2221 aaatcgataa aatcaatata ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa
     2281 tcctgcatag atgacaacgt atgctaggat tcaggcgtct tctttttaca taaaaagcaa
     2341 gtaagcttcc ttttgttttg ctatatcagg ttttatagtc aagcattttg gttgacgatg
     2401 aacgtgaact cgcactcaca gactttttta tcatctacat atgctgcaga taacgctgta
     2461 ccgactgatc ccttcatttt ggtgatttca aaatgacatt tcagcacatc tcctggaaca
     2521 actttttgac ggaatttggc cttattgatc ccaccgatat aagctctttt tcctagaaac
     2581 tcttctgatt ttaggataag tatcgaacct gcttgtgcca gtgtttctag aatcaacgca
     2641 cccggcatca ctggattgtc aggaaagtga ccttggaaaa aatcttcatt gattgttaca
     2701 ttttttgtcg caacgatctt ttttcctggt tctaaagtat ctacataatc aatataacaa
     2761 ataggataac gattaggaat caattccatt acttcagtag cagataatac gtaagacata
     2821 attaaactcc ttctttcagg caattgccat tttcattact ttgactatca aagtatcata
     2881 ttcctaaata tttagcaatc tttttttaat ttaagaaaaa atagtttgac aatcaaaaca
     2941 ttttgactaa taattaagaa atcaagaagt tattttagct attattctct atttttttac
     3001 gactatcaaa tttttatcag aaacaaagga gagtcattat gtcatttcta gaaggaaaaa
     3061 aaatcgtcat tatgggcgtc gctaacaaaa aaagcattgc ttggggttgt gctgaagcaa
     3121 tgttgaaaca aggggcagaa attatctata cttaccaaaa tgaacgaatg aaaaaatcat
     3181 tagtcaaatt ggtcggcgaa gaagcattca ctgtagaatg tgatgtttct agcgacgaaa
     3241 gcatcgaaaa agcaatgaat gccatcggtg aatatgctgg ggagatccac ggccttgtcc
     3301 atgcagttgc ttacgccaac aaagaagaat tgtccggaaa tgtgtctgat atttccagag
     3361 acggctatca attagctcaa gatatcagca gctactcttt aattgctgta tcaaaatacg
     3421 cacaaccgtt tcttgcacca aacagcggga tcgttagcat gacttatcta ggttcagaac
     3481 gcgcaatccc gaattataac atgatgggta ttgcgaaagc ttctcttgaa gctgctatcc
     3541 gttatttggc agctgagttc tcgcctaaag gaattcgggt caatggaatc tcagctgggg
     3601 caatcaaaac attagctgtc acaggtgtta aagactatca aaaactgatc cagttatctg
     3661 aatctcgtac gcctgatcag caaggtgtga cgatcgaaga agtcggtaat gcttgtgctt
     3721 tcttaatcag cccactttcc agcggaatca tcggtgatat tatctacgtt gacaaaggtg
     3781 tccatctttc ataaactttt gttgcattct ttaaagtaaa acatataaaa atcaggaaaa
     3841 aactttttaa agtctcttcc tgattttttc tttttacgtt tttttcaact cattactttg
     3901 aaattattcc acgacttcaa tccagccttc tggtgcttct atgtctccaa attgaatacc
     3961 tgtcaattca tcatacaacc gttttgttac tggtcctact tccgtttcac tataaaatac
     4021 atgaagattg ttattgtgat aaatcccacc gattggagaa ataacagccg ctgtaccaca
     4081 tgcaccagct tctgcaaatt gatctagatc atcaatgaaa atatctcctt caattgcttg
     4141 cagtcctagt cgttcttcag ctaactgcaa caaggaatat ttcgtgatac ttggtaagat
     4201 agagggcgat tgcggagtga tgaattttcc gtcttttgta attccaaaga aatttgctgc
     4261 acctacttct tcgattttcg tatgtgtggc tggatcaaga taaacacaat ctgagtaatc
     4321 cagttctttt gcttctgttc ctggaagtaa actcgctgcg taatttccgc cgacttttgc
     4381 tgcaccagtc cccttaggag cagcacgatc gtaatcagat acaataaaat tcgttggttt
     4441 tagtccacct ttgaaatacg ctcctactgg cgtacagaag acgacaaata aatattctgg
     4501 agctggatgg acaccaatgt tttcaccgac cccaatcagt aaagggcgca agtataatgt
     4561 agcgcctgtt ccatagggcg gcacaaaatc ttcatttgct tttaccactt gcttgacagc
     4621 atcaacgaac atcttttcag ggaccattgg catcagcaat cgtctagcac tttgattcat
     4681 ccgtcgacta ttttgttcag gtcgaaagag attgaccttg ccgtcttttc gacgataagc
     4741 ttttagacct tcaaaacatt gctgaccata atgaagcgct ggagagcctt catgaatcgt
     4801 caggtaatta tcttctgtca attctccttc ttcccattgt ccgtctttcc atttggcaat
     4861 aaaacgccaa ggagttttga tataagaaaa acctagattg ttccaatcga tttgcatata
     4921 cttaccacct tcgttatttt ctccacattt tatcattatc catattattt gtcattttat
     4981 tttcagcttt tttccttcat tatagttatt tttgctattt ccttttttga aaacttcttg
     5041 gtacaataca gaaagaatca aattttagaa aagaggattt tatgttttca ttattagcaa
     5101 ctgccggtac gacagactct tctggcgaga cattgacaga tcaagctgtc agcaaagtaa
     5161 atgcgtttca gcggttttgg gacgggattg attgggacgc cattttggct gcctttatcc
     5221 aaaaaagttt atatctgatt tttttgatcg tccttttttg gattcttcac cgtgtgggaa
     5281 aatatctgat tgataagtct tttaatcagt attctaaaaa agccattcta aacgagagcc
     5341 gcttaaagac tttacatagt cttttaaata ctatttttca ttataccctt ggtttctttt
     5401 ttatctatgc aattttatct gctatcggtg tgccgatcgg atcacttttg gctggtgccg
     5461 gaattgcagg tgtggcaatc ggtcttggtg cccaagggtt catgagtgat gttattaccg
     5521 gattttttat tatcatggaa caacagatgg atgttgggga ctacatcaag ctagcaaact
     5581 taacgattga aggaaccgtc gtatctgttg gtatccgtac ccttcagctg aaagcaacgg
     5641 atggtactgt ccatttcatc ccgaatcgaa acatcaccac gatcagcaat ctctctcgtg
     5701 caaatatgca agtactttta gatattcgga ttgttcctga agaaggatat gacaaaatct
     5761 atgagatcat tgatcgagta aatcgtacat tagctgaaaa ataccaagaa gagctccaaa
     5821 ctgaaccttc tatctttgga ttagtggatc ttggaaacag caattttgcg attcgtacgg
     5881 tctgctacgc attgaacgga aaacaatatg ccttaaaaga agagttcttg agctcctatg
     5941 tcaaagaatt gacagcttct ggtttcacga ttccgaacag tccaattgct gtcggaaaat
     6001 aatttggaca aacgatgcaa cgaatctcac taaaaagagc gtgggacaaa agtaaagaac
     6061 acttttgtc
//
LOCUS       DACDYM010000107         6040 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDYM010000107 DACDYM010000000
VERSION     DACDYM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.111"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(254..541)
                     /locus_tag="IWM59_002469"
     CDS             complement(254..541)
                     /locus_tag="IWM59_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9980458.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="IWM59_002470"
     CDS             complement(561..785)
                     /locus_tag="IWM59_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9980459.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1425..1958)
                     /locus_tag="IWM59_002471"
     CDS             complement(1425..1958)
                     /locus_tag="IWM59_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9980460.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="IWM59_002472"
     CDS             complement(1979..2923)
                     /locus_tag="IWM59_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9980461.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="IWM59_002473"
     CDS             3560..3646
                     /locus_tag="IWM59_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9980462.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="IWM59_002474"
     CDS             complement(3767..4456)
                     /locus_tag="IWM59_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980463.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="IWM59_002475"
     CDS             complement(4463..4741)
                     /locus_tag="IWM59_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980464.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="IWM59_002476"
     CDS             complement(4701..5615)
                     /locus_tag="IWM59_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9980465.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="IWM59_002477"
     CDS             complement(5597..5977)
                     /locus_tag="IWM59_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9980466.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1594 a         1114 c         1017 g         2315 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag
//
LOCUS       DACDYM010000108         5962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDYM010000108 DACDYM010000000
VERSION     DACDYM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.112"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..450)
                     /locus_tag="IWM59_002478"
     CDS             complement(<1..450)
                     /locus_tag="IWM59_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9980467.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IWM59_002479"
     CDS             674..1465
                     /locus_tag="IWM59_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9980468.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IWM59_002480"
     CDS             1431..2384
                     /locus_tag="IWM59_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP9980469.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IWM59_002481"
     CDS             complement(2782..3756)
                     /locus_tag="IWM59_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9980470.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IWM59_002482"
     CDS             3871..4305
                     /locus_tag="IWM59_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9980471.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IWM59_002483"
     CDS             complement(4430..5416)
                     /locus_tag="IWM59_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP9980472.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..>5962
                     /locus_tag="IWM59_002484"
     CDS             5844..>5962
                     /locus_tag="IWM59_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP9980473.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIET"
BASE COUNT         1873 a         1124 c         1027 g         1938 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag ac
//
LOCUS       DACDYM010000109         5904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDYM010000109 DACDYM010000000
VERSION     DACDYM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.114"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..986)
                     /locus_tag="IWM59_002485"
     CDS             complement(<1..986)
                     /locus_tag="IWM59_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP9980474.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1138..1833
                     /gene="vanR"
                     /locus_tag="IWM59_002486"
     CDS             1138..1833
                     /gene="vanR"
                     /locus_tag="IWM59_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9980475.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1811..2965
                     /locus_tag="IWM59_002487"
     CDS             1811..2965
                     /locus_tag="IWM59_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9980476.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3180..4148
                     /locus_tag="IWM59_002488"
     CDS             3180..4148
                     /locus_tag="IWM59_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9980477.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4141..5172
                     /gene="vanA"
                     /locus_tag="IWM59_002489"
     CDS             4141..5172
                     /gene="vanA"
                     /locus_tag="IWM59_002489"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9980478.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5178..5786
                     /locus_tag="IWM59_002490"
     CDS             5178..5786
                     /locus_tag="IWM59_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9980479.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1638 t
ORIGIN      
        1 tttccgataa ccgtgtattg aaaggcatct tcgaagccat tatccaatat ttcgcaggct
       61 tttgtatatt taggctggtc gatatattca ccgactaagg cattcttagc tgttcgagcg
      121 agttcaatat ccgtaaactt aaagatcgct tttactgctt ctctaaacgg ttttgaattc
      181 ttttttggaa tggaactgaa gatgtttctt aaaaaatgga cctggcatct ctgccaactt
      241 gcgttggtaa atgacttacg aatcgcagac actaggcctt tatgggcatc agaaatgatg
      301 agttctgtcc cctttaggcc gcgttctttt aagtattcaa agaagatgga ccatgtgtca
      361 tcactttctt cattttgaat catgaagcca atgatttcac ggtcgccacc ttctgttatc
      421 ccgatcgcaa tatggcagct tttagaaagc actcgatggt cctcacggac ttttatgtag
      481 agaacatcag tcatcagata aggataattc gtacctgaga gtgaacggtt ctgccattcg
      541 ttgaccatcg ggtctaactg ctcagtcagg ctagaaacaa aagatttcga tacagatttt
      601 ccacatagct cttcaacaat ctttgaaact ttacgtgtcg aaacgcccga aacatacatc
      661 tcaagcattg aagcgagcag tgccttttca tttcgctgat aacgctcaaa caccgtcggt
      721 gaaaattcac catcacgtgt tctaggcact tttaattcga gtgtacccac acgagtcgta
      781 aagtctcgct cataatagcc atttctttga ctctgacgac tttctgaacg ttcatagtca
      841 tcggcttgaa tatattctgt tcgttgattt tccatcaatt ggttgaaaac agtggttaaa
      901 atatttttag aaacatcatc ttttaccgaa tgttcaataa tactttgaac ctcttcgttg
      961 ttcagtgtaa aatgtacttg ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa
     1021 aacattgtac cgtaaaaggg ctgttacatg gccttttatc ttttacacaa ttatatggac
     1081 tttatcctat aatctgataa attattaagg gcaaacttat gtgaaagggt gataactatg
     1141 agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt tgaattatac
     1201 ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc attggaatgt
     1261 atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg cacaagcggc
     1321 cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat gctgaccggg
     1381 aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga ttatataacg
     1441 aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg ccgatacaaa
     1501 aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg ccttgtcatt
     1561 aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac tcccaccgag
     1621 ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc cgagctgcta
     1681 tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat caccgtgcat
     1741 atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata tataaaaacg
     1801 gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca aactagaacg
     1861 aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg tgttgtatat
     1921 tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg aaaacaaata
     1981 tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga acaatataga
     2041 tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg tcatgctttc
     2101 aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta ttcagaacga
     2161 agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt
     2221 aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga
     2281 cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta tcggttattt
     2341 gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat
     2401 cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg agattacacg
     2461 gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact atatgctggt
     2521 gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg cggttattca
     2581 cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag tctttaacaa
     2641 cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca ttaccgcggg
     2701 cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc caaaagataa
     2761 gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt ccgatacggg
     2821 tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg gagggcagat
     2881 ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag cgatgccaga
     2941 cttggttgat aaaaggaggt cctaagagat gtatataatt ttttaggaaa atctcaaggt
     3001 tatctttact ttttcttagg aaattaacaa tttaatatta agaaacggct cgttcttaca
     3061 cggtagactt aataccgtaa gaacgagccg ttttcgttct tcagagaaag atttgacaag
     3121 attaccattg gcatccccgt tttatttggt gcctttcaca gaaagggttg gtcttaatta
     3181 tgaataacat cggcattact gtttatggat gtgagcagga tgaggcagat gcattccatg
     3241 ctctttcgcc tcgctttggc gttatggcaa cgataattaa cgccaacgtg tcggaatcca
     3301 acgccaaatc cgcgcctttc aatcaatgta tcagtgtggg acataaatca gagatttccg
     3361 cctctattct tcttgcgctg aagagagccg gtgtgaaata tatttctacc cgaagcatcg
     3421 gctgcaatca tatagataca actgctgcta agagaatggg catcactgtc gacaatgtgg
     3481 cgtactcgcc ggatagcgtt gccgattata ctatgatgct aattcttatg gcagtacgca
     3541 acgtaaaatc gattgtgcgc tctgtggaaa aacatgattt caggttggac agcgaccgtg
     3601 gcaaggtact cagcgacatg acagttggtg tggtgggaac gggccagata ggcaaagcgg
     3661 ttattgagcg gctgcgagga tttggatgta aagtgttggc ttatagtcgc agccgaagta
     3721 tagaggtaaa ctatgtaccg tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc
     3781 atgtgccgct caatacggat acgcactata ttatcagcca cgaacaaata cagagaatga
     3841 agcaaggagc atttcttatc aatactgggc gcggtccact tgtagatacc tatgagttgg
     3901 ttaaagcatt agaaaacggg aaactgggcg gtgccgcatt ggatgtattg gaaggagagg
     3961 aagagttttt ctactctgat tgcacccaaa aaccaattga taatcaattt ttacttaaac
     4021 ttcaaagaat gcctaacgtg ataatcacac cgcatacggc ctattatacc gagcaagcgt
     4081 tgcgtgatac cgttgaaaaa accattaaaa actgtttgga ttttgaaagg agacaggagc
     4141 atgaatagaa taaaagttgc aatactgttt gggggttgct cagaggagca tgacgtatcg
     4201 gtaaaatctg caatagagat agccgctaac attaataaag aaaaatacga gccgttatac
     4261 attggaatta cgaaatctgg tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa
     4321 aacgacaatt gctattcagc tgtactctcg ccggataaaa aaatgcacgg attacttgtt
     4381 aaaaagaacc atgaatatga aatcaaccat gttgatgtag cattttcagc tttgcatggc
     4441 aagtcaggtg aagatggatc catacaaggt ctgtttgaat tgtccggtat cccttttgta
     4501 ggctgcgata ttcaaagctc agcaatttgt atggacaaat cgttgacata catcgttgcg
     4561 aaaaatgctg ggatagctac tcccgccttt tgggttatta ataaagatga taggccggtg
     4621 gcagctacgt ttacctatcc tgtttttgtt aagccggcgc gttcaggctc atccttcggt
     4681 gtgaaaaaag tcaatagcgc ggacgaattg gactacgcaa ttgaatcggc aagacaatat
     4741 gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg
     4801 ggaaacagtg ccgcgttagt tgttggcgag gtggaccaaa tcaggctgca gtacggaatc
     4861 tttcgtattc atcaggaagt cgagccggaa aaaggctctg aaaacgcagt tataaccgtt
     4921 cccgcagacc tttcagcaga ggagcgagga cggatacagg aaacggcaaa aaaaatatat
     4981 aaagcgctcg gctgtagagg tctagcccgt gtggatatgt ttttacaaga taacggccgc
     5041 attgtactga acgaagtcaa tactctgccc ggtttcacgt catacagtcg ttatccccgt
     5101 atgatggccg ctgcaggtat tgcacttccc gaactgattg accgcttgat cgtattagcg
     5161 ttaaaggggt gataagcatg gaaataggat ttactttttt agatgaaata gtacacggtg
     5221 ttcgttggga cgctaaatat gccacttggg ataatttcac cggaaaaccg gttgacggtt
     5281 atgaagtaaa tcgcattgta gggacatacg agttggctga atcgcttttg aaggcaaaag
     5341 aactggctgc tacccaaggg tacggattgc ttctatggga cggttaccgt cctaagcgtg
     5401 ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa taacctgaca aaggaaagtt
     5461 attatcccaa tattgaccga actgagatga tttcaaaagg atacgtggct tcaaaatcaa
     5521 gccatagccg cggcagtgcc attgatctta cgctttatcg attagacacg ggtgagcttg
     5581 taccaatggg gagccgattt gattttatgg atgaacgctc tcatcatgcg gcaaatggaa
     5641 tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc catcatggaa aacagtgggt
     5701 ttgaagcata tagcctcgaa tggtggcact atgtattaag agacgaacca taccccaata
     5761 gctattttga tttccccgtt aaataaactt ttaaccgttg cacggacaaa ctatataagc
     5821 taactctttc ggcaggaaac ccgacgtatg taactggttc ttagggaatt tatatatagt
     5881 agatagtatt gaagatgtaa ggca
//
LOCUS       DACDYM010000110         5836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDYM010000110 DACDYM010000000
VERSION     DACDYM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.115"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(192..662)
                     /locus_tag="IWM59_002491"
     CDS             complement(192..662)
                     /locus_tag="IWM59_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9980480.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="IWM59_002492"
     CDS             992..2632
                     /locus_tag="IWM59_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9980481.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="IWM59_002493"
     CDS             2786..4108
                     /locus_tag="IWM59_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9980482.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4610)
                     /locus_tag="IWM59_002494"
     CDS             complement(4176..4610)
                     /locus_tag="IWM59_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9980483.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4675..5511)
                     /locus_tag="IWM59_002495"
     CDS             complement(4675..5511)
                     /locus_tag="IWM59_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9980484.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1747 a         1164 c         1070 g         1855 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4321 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4381 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4441 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4501 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4561 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4621 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4681 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4741 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4801 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4861 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4921 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4981 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     5041 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     5101 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5161 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5221 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5281 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5341 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5401 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5461 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5521 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5581 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     5641 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     5701 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     5761 agaaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaaca
     5821 gcttctcatg tttgtt
//
LOCUS       DACDYM010000111         5726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDYM010000111 DACDYM010000000
VERSION     DACDYM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.116"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            196..921
                     /locus_tag="IWM59_002496"
     CDS             196..921
                     /locus_tag="IWM59_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980485.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IWM59_002497"
     CDS             911..1420
                     /locus_tag="IWM59_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980486.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="IWM59_002498"
     CDS             complement(1490..2938)
                     /locus_tag="IWM59_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9980487.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="IWM59_002499"
     CDS             complement(2938..3654)
                     /locus_tag="IWM59_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9980488.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="IWM59_002500"
     CDS             complement(3635..3985)
                     /locus_tag="IWM59_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9980489.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="IWM59_002501"
     CDS             4129..4902
                     /locus_tag="IWM59_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9980490.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="IWM59_002502"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="IWM59_002502"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1661 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttt
//
LOCUS       DACDYM010000112         5638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDYM010000112 DACDYM010000000
VERSION     DACDYM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.117"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            58..510
                     /locus_tag="IWM59_002503"
     CDS             58..510
                     /locus_tag="IWM59_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980491.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            524..1213
                     /locus_tag="IWM59_002504"
     CDS             524..1213
                     /locus_tag="IWM59_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980492.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1338..3689
                     /locus_tag="IWM59_002505"
     CDS             1338..3689
                     /locus_tag="IWM59_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9980493.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3769..4164
                     /locus_tag="IWM59_002506"
     CDS             3769..4164
                     /locus_tag="IWM59_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980494.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4489..5106
                     /locus_tag="IWM59_002507"
     CDS             4489..5106
                     /locus_tag="IWM59_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9980495.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5147..5446
                     /locus_tag="IWM59_002508"
     CDS             5147..5446
                     /locus_tag="IWM59_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980496.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5480..>5638
                     /locus_tag="IWM59_002509"
     CDS             5480..>5638
                     /locus_tag="IWM59_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9980497.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVH"
BASE COUNT         1973 a          747 c         1147 g         1771 t
ORIGIN      
        1 caattatctt atcgtagata tatattataa atttaataag aaagcgaggt gagaatcgtg
       61 aaaaaaacga tactaagtat tttttcagta tttacaatat taattggggg ctattttctt
      121 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
      181 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      241 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      301 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      361 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      421 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      481 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      541 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      601 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      661 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      721 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      781 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      841 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      901 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      961 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
     1021 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
     1081 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1141 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
     1201 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
     1261 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
     1321 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
     1381 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
     1441 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
     1501 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
     1561 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
     1621 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
     1681 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
     1741 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
     1801 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
     1861 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
     1921 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
     1981 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     2041 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     2101 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     2161 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     2221 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     2281 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     2341 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     2401 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     2461 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     2521 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     2581 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     2641 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     2701 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     2761 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     2821 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     2881 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     2941 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     3001 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     3061 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     3121 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     3181 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     3241 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     3301 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     3361 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     3421 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     3481 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     3541 taatttcatt tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc
     3601 ttgattttat tggagatgag gggaaagttg caaatggatt ttctgaaaaa attattcaat
     3661 ttcaagaaaa tagaagtaaa cttaattaag aatcgtttac tatttaaaca gtgtcctttt
     3721 cctttttaaa tatatacgat aaaataagta tatgaattgt aaaaggacat gatgattatg
     3781 gaagaaatag aatggaataa tctgactcaa aataataaaa ataattctgg atgtggaatt
     3841 atctttatgt ttgttatagt tttgggaata gtagtacttt taataactgt caagatagta
     3901 gattactttg cgaatactcc tgaagaacaa atacagagaa aacttcctga gtttgaaaga
     3961 gtaccacctt atgatccaag agataattca tttcaagtaa aaaaattaaa agagtctcca
     4021 aatgaaatgt ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa aaagaaagaa
     4081 tacaattata ttgtaaaggc aaaagtatca aaaagattta tttttaaccc taaattagaa
     4141 atatatagtg ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa
     4201 attctgatgt gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag
     4261 tttatttaat ggcagaccgt taaggcctgc catttttatg tattttagcg gaataaatat
     4321 aatcatgcgg gagtgtaaac ttactgtttc aaaaagtata ctttttgaca tgccttgatt
     4381 ttctttgttt atgtatggca taataaccac ataatacaat ttgaaacgtt tctgatttaa
     4441 tctgattgtt ttgaaacaat tttagtgaaa aaagggtaga taaaaacgat gagaaaattt
     4501 ttttatatcc gggtcagtag ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag
     4561 gaattaggaa tccctgaaga atatatattt gtagaaaaag ccagcggcaa agattttaaa
     4621 cgccctgaat atcaattaat gaaacggatg tttcgaaaag gagatttact ctatctacaa
     4681 tcattagata gactaggcag aaataaacaa atgattttag aagaatggcg agaattaact
     4741 aaggaaaagc agattgatat ttctgtgtta gacatgcctc tgttagatac tgctagttac
     4801 aagcaactag atgggttaga gacgcttatc tctgacttaa ttctgcaatt actcagctat
     4861 atggccgaag acgagcggaa acgaattaat agccggcaaa gagaaggcgt agaagctgcg
     4921 aaacaaaagg gtgtaaaatt cggcagaaag aaaattgctt tacctcctga atttcctgcg
     4981 atctattctg actggaaagc aggaaagata actgcagtct atgctatgga tcaattgggc
     5041 atgaaacgaa atacctttta tcgacgggtg aaagaatatg aacatacctt gcaccacttt
     5101 tcttgataga tataacagct attagacggg aagggagagt ttgaaaatgg acttaacgaa
     5161 tcaaagggtc gtttttactg gaacgctgca gcaaatgaca cgaacccaag ccatcggttt
     5221 ggttcactct ttaaacggcc attgtcaatc aagtgttact tcaaaaacaa atttcctggt
     5281 ttgtggtcag tattctaagt cgttgtttga tgattctctc tatacaaaaa aggaacaaac
     5341 cgcgcgggac ttgattgcgt taggtcatga gattaccatt ttatcagaag tcgaatttta
     5401 tgcgttaatt agtcaacagt tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt
     5461 tacaaggagg aaaattttta tgaagcgaaa atcttatgat cgttggggca ttgaaataac
     5521 tagttttaat cgaaatattg cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag
     5581 tgtttatcaa aaaaattact tttactgtcc taaatgccat cgccgatacg gggtacat
//
LOCUS       DACDYM010000113         5584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDYM010000113 DACDYM010000000
VERSION     DACDYM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.118"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(487..1296)
                     /locus_tag="IWM59_002510"
     CDS             complement(487..1296)
                     /locus_tag="IWM59_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9980498.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1296..2042)
                     /locus_tag="IWM59_002511"
     CDS             complement(1296..2042)
                     /locus_tag="IWM59_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9980499.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2060..2527)
                     /locus_tag="IWM59_002512"
     CDS             complement(2060..2527)
                     /locus_tag="IWM59_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9980500.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2527..2937)
                     /locus_tag="IWM59_002513"
     CDS             complement(2527..2937)
                     /locus_tag="IWM59_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9980501.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2948..3961)
                     /locus_tag="IWM59_002514"
     CDS             complement(2948..3961)
                     /locus_tag="IWM59_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9980502.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4178..4912
                     /locus_tag="IWM59_002515"
     CDS             4178..4912
                     /locus_tag="IWM59_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9980503.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4896..>5584
                     /locus_tag="IWM59_002516"
     CDS             4896..>5584
                     /locus_tag="IWM59_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9980504.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1789 a         1184 c          920 g         1691 t
ORIGIN      
        1 agcgacaaaa accacgagca ggtccattca tcctcaaacc tcggtgatct tcgcggttta
       61 acaccatcag aagaaatgca caaaaataat aaggagagtc tgagacataa ataaattccc
      121 agacttttag tgagtctaaa tccgaataaa cggtgggtcg aaagcaactc tagctgcgcg
      181 tcgatcgcag caatctctag gtgctatgtt cggataaatg gtggtcaaaa gtcagatttt
      241 cggccatttc gtaacagcca accagacaaa aagcgtctgt tttgtcttct actccaattg
      301 ctcaaatctt agcgactttt ggcttggtct tactctaaac agtttgtaag cctaaacttt
      361 atttgctgtt tgtttctaag atcaaagcaa aactactttt tagttggtta cttttacaag
      421 ttctttttac tgttgaataa atgtaaatct taagaacaaa tatttacttc tataattcca
      481 agtttcttaa aaaattccgc caagtgtacc taaaaagcat aaaacaaaaa ttcctagaat
      541 aattgtgatc gttttatgcc ctttggagta aagccagacg cataaaaagg taattcctaa
      601 tggcagcaag cccggaaaaa tttgatcaaa aatactttgt aatgaagttt cagctccacc
      661 aaccgtgaat tttagtgacg tatttaaagt aaccatcgtg cctatcatcg cgccaattac
      721 cgttaaacct aaaatcgaag caccatgaga taatttgtca atcatatggt tgtcttctgt
      781 atcatcaata aatttagttc ctaaaacata gccatatttt gtcgccaacc atttaaaaat
      841 aaaatgaatg gcagtataca aagtaaaata aaggatcatg cctaaaatat tcccttgttg
      901 agcaaaagaa atacctatgc cagccgcaat aacccgaaaa gttccccaga aaaatgaatc
      961 gccaatacct gataaaggtc ccattaatcc tgttttgatt ttattaattg cgtctgtatc
     1021 aaattcagga tttttagcat tttcttcttc catggtgacg gacaagctca taacaaaagg
     1081 cgcaatatgc ggtgtaatgt taaatgtttc cgtatggcgc tgcaacgctg tataataatc
     1141 atcatcgttc ggatatattt tacgtaagga acgttcaata ccattcattt gtccatatgc
     1201 catcatttta tcgaaggacc aagaggattg cataaaaaag gagcgccaaa atatacttct
     1261 taattctttt ttcccaataa cactttcttt taccattata aatcttcctc ctcttcaaaa
     1321 gttagctttt gaacagccag taacgctaag caaagtccga cgacagccac accgacaaca
     1381 tccattttca gatacattac gccgacaaat cctaagaaaa gataaggcat catcgcttta
     1441 ttcgccatca ttttaattaa taacgctagc ccaacacaag aaagcacacc ggccgcaaca
     1501 tcaaagctgt ccaatacaac ctgaggaatc aacttgatca tcgcattgat tgtagacaca
     1561 ccaaaataag tagctacaaa aactaaaatg gccgatggaa ccccaacgat taaaggttga
     1621 gccacataat gatatttttg tgcttttttg aaatcatgat tgactaatgc ctcatccatt
     1681 ttgttgttaa aatacgtaat tggcattgca taggcgaagt tacgccacat ctgaagaaag
     1741 atggacaaag gtaaagtcaa cgcaacggca gttcctactc cttgacccga ttgaatcgca
     1801 attgctgtac ctaaaacact ggaagcataa acttcagggg gcgtcgcact ccctaccata
     1861 attgagccca tgaacatgga ttccaaggtg gcaccaataa taattcctga ctctaaatct
     1921 cccaaaatca aaccgacaaa aggcgctgta aatagcggtc tacccatcat cgatgcacca
     1981 aatgtgtatt catcaataga cgccaaaaaa gctaccagtg cgattaatat tgcttgtatc
     2041 atattcttcc cctcctcttt tagtcatgca aaggattgat ttttttgtcc gttggtacag
     2101 cctgacattg aactgttatc ccgttttcag tcattttttt caccaagtcc agttcttgtt
     2161 cattcaagta aacggaacta gtaatcattt tccgattctc gtttttcttt aaatttccta
     2221 aattcacttc tttaatctca ggtacgtgct gagtaatttc gtaggcatcc tccaccgtcc
     2281 cgacaattaa aaatacttgg tatttcttga ttttttctcc atttaaaaca gagatggcct
     2341 ctgtcatatc cttcaccaca aattttgtcc ctgtaggaac agccattttt agtgtttttt
     2401 tgcgcagatt attggtagcg atttcattac ttgcaacgaa aatacaattt gcacctaagt
     2461 tatttgtcca ggcaaaagca acttgcccat gaacaaaacg ttcatctact cgtaataatt
     2521 taatcattaa aaatcatcct cttctgtttt ctctagcaac tcgttcacca aaactatccc
     2581 ttcattcccg ctattcatag ctttttggat tagttttttc gtgtcctgtt cacctgataa
     2641 gcaaagctct aaaagcagcg gcagatttac accgctgatt aaataaaaat tgtctttacc
     2701 gatttgttcg gagaaagcgt tagacacgct tcctcccagt aaatctaccg tcacaattaa
     2761 ttcgtcatca ggcgctaagt cggaaaatag ctgctctatt tcctggttaa tggagtagtc
     2821 actatctata tacgcagtta ttgctaaaag tgatttttga ggaccaataa taaattctaa
     2881 agtatccttt aagcctttag ccatcatgga atgcgtcgca attacaattt tcctcatagc
     2941 tcttacctta atattccaca gtccgataat aacgacgaat atctagtgaa tgatcccgca
     3001 ccttttcaaa atgagcactc actcgttcta aggccgttgc cataaccatt ggcgctacat
     3061 actgacgcat agcgcttggt acacctgcta attcgaaatc cgcagtgtca aaaaccgtca
     3121 caacatcgct atatttattt acaaaacgct ctacgcgatc gactagcgga cgcgtttgat
     3181 cttcaccttt taataacaca aaactcatag atttatcagt catttcaata gtcccatgga
     3241 aaaattcggc tgcatggatc gacttggtgg caatccattg catttcttcc agtacgcaca
     3301 tagcatattc ataggcagcc ccataagtat tcccggctcc aacaaacata tgaaattccg
     3361 tatttttatg tttttctgca aattcaagtg ctttctgatc attttgttgc cgaacactta
     3421 gtaatacttc cggcattttt tgtaatgttt gaataaattc atcatattca ggaaactcac
     3481 cgttattttt catcaggcgt gcacccaaag caaaaaactg taactgaagt tcttctacta
     3541 atgtatcgtt gtcagcatca ttttcaatcg cataatccac gatttccccg agtggtgtag
     3601 cagcatttcc tacaaacgca atcgtcgttg caccttgact attggcatac ttaacaacat
     3661 caacggtttc ttgagtcgta ccggttaatg aagatgtgat taacactgta tctttattaa
     3721 tctttttcgg ctgtgaacaa accacctctg cggctatttc atgataataa tcaagctctg
     3781 aatgagtgct taacagataa gctagcgggc tgattgtggc atatgttcca ccagagccta
     3841 ccaaataaag atttttaaat cccttctcac tgattgcatc tgccacctga ttgattgtct
     3901 cgatattttt tactgcacca tttaatactg atagaaaacg ttcttcgcta aaacctaaca
     3961 tagttaaaaa cctccaagtc aataatttga aaaatatatt tgcaaatgcc ggcacattaa
     4021 caaaccactt aacctttttt atataccacc tgttaacgtt tttaatgtac cacttataga
     4081 aaacgctgtc aatagttttt aaccactttt atttttattt gtaccaaaat ccttttttca
     4141 tgtataataa acaaaaatag actctggagt gtaacgtatg aatactgaag aactaacgaa
     4201 caaattaata caagacattg aacaagaaaa atataaatac gcgcaagcta ttccttcaga
     4261 acgtgatttt gttcaaattt atgaaatgaa tcgttcagcc gtaaaaaaag ccattcaaca
     4321 attagtaaat aaagagtacc tactaaaaat acatggcaaa ggaacttttg ttaaaaaaaa
     4381 tatccatgaa catttgcaaa tcaaatttca tggtttgtct gaattgttgg aacgggaagg
     4441 gattcagcct agcagcttaa ttttagcttc agaaataaaa ccggctggac acattgtcgg
     4501 gaaagcttta cagctggaac caactgaaga agtatttcat ttggttcgtt taagaagcgg
     4561 cgacggtgag ccaatttcta ttgaagacac ttatattcca ttaaatctca tacctgatct
     4621 tcaagctttt gatttccaaa ttttttcatt ttattccaca ttggctgctt atcattatca
     4681 aattgaaaat atctatcaga caatttccag ctcaaaagtg agcaataaag aagcccgttt
     4741 gttaaaaacc agcttaaatc gtccggtaat cagccttgct atcactgcgg ttacgacaaa
     4801 tgaacaagcg gttgaataca ctgaagtagt ggttttgcct gaattcgccg ccatctatac
     4861 taaaaatgtt catcaaggaa aggagatgaa aatctatgcc caaattgatt aatctgttgg
     4921 cttatgacct gattgacaaa attattgctt atattcatga acatcattta acccccggaa
     4981 caaaactgcc ttcggaacgg gacctatcag aaatgtttaa tgttaatcga actaccttaa
     5041 gaggcgcgtt acaaaaactg gtggacaacg gcaccttacg ggcgcaaacc aatagcggct
     5101 attatttaac tgcgccaaaa ttcgaaaaaa atgcccatca tttctttaca ccgatagaag
     5161 attctctgct tagtaagcag cggctacagc taaaggcaac ggctctttca ccgaaaaaag
     5221 cagcagctct ttgtccggaa ctgaatgatt tcaaacagca gcaaccacaa gcttatttag
     5281 aatatattga tgcagaagca attagcttga tgttattaat ccctatggaa acattaaaga
     5341 aaagtaattt ctcggacaat cctttccaag attttaaaag tttggcatat acgcgttctc
     5401 aagaaatttc tttaataact gacaatgacg ttgcggcgga cttgctggga gttgacaaag
     5461 aagaggttct gttatttgta cgagaggaaa taagagccgg taaggagttg gcggcgattg
     5521 ctttaggtta tactattagt cgccgctgca acatacaggt taatgttgct acgaccttgc
     5581 aatc
//
LOCUS       DACDYM010000114         5548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDYM010000114 DACDYM010000000
VERSION     DACDYM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.119"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(62..613)
                     /gene="raiA"
                     /locus_tag="IWM59_002517"
     CDS             complement(62..613)
                     /gene="raiA"
                     /locus_tag="IWM59_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9980505.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(741..1460)
                     /locus_tag="IWM59_002518"
     CDS             complement(741..1460)
                     /locus_tag="IWM59_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9980506.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(1457..2770)
                     /locus_tag="IWM59_002519"
     CDS             complement(1457..2770)
                     /locus_tag="IWM59_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9980507.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(2851..3171)
                     /locus_tag="IWM59_002520"
     CDS             complement(2851..3171)
                     /locus_tag="IWM59_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9980508.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(3279..3566)
                     /locus_tag="IWM59_002521"
     CDS             complement(3279..3566)
                     /locus_tag="IWM59_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980509.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            3693..4337
                     /locus_tag="IWM59_002522"
     CDS             3693..4337
                     /locus_tag="IWM59_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9980510.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            complement(4397..5104)
                     /locus_tag="IWM59_002523"
     CDS             complement(4397..5104)
                     /locus_tag="IWM59_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9980511.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
BASE COUNT         1588 a         1161 c          958 g         1841 t
ORIGIN      
        1 aaaacttgcg gcagctgtca gtagctgctg caagttttcg ttgttattta agtaaggctg
       61 atcagtctgt ttcaatcagt ccgtattttc catctttacg gcggtaaacg atacttgtac
      121 catttgtttc tgcatcttca aaaataaaga agttatgtcc cagcatgttc atttgtaaga
      181 ctgcttcttc actatccatt ggtttcaatg aaaggcgttt cgtacgtacg atatctaatt
      241 cagaatcatt ttcgttttcg tcctcttcat tgaataaagc agtagcatct gcagtattca
      301 tgcccgtttc acgggattta cgattaattt ttgttttgaa tttgcggatt tggcgttcca
      361 atttgtcaac aaccaaatcg atacttgcgt ataaatctgg agaagtttct tcggcacgta
      421 atacaagata aggtaatggg atcgttactt ccacttttgc tgttttttct gtataaactt
      481 ttaagttcac atgcgccgtt gcatcgggtg cgtcactgaa atagcgttct agtttgccta
      541 cttttttctc aacgtagtcg cgaatcgctt cagttacttc gatattttcg ccgcgtacat
      601 tatatctaaa cataaaaatt acccctttcg tctaccacaa tcaagaagga gaaggaccac
      661 tctttccctc ttcttaattc tattatatcg aactctaggt tatttgcaaa ttaaatcgct
      721 atcaaatttc cacatctaat tcatcttgct aaagaaatgg ttcggatgat tttcggctgt
      781 gcgacctgca aacaatctgc tgcgtggaaa agagtctgtc ctgttgtata gacatcatca
      841 ataatcaata tttttttatt ttgtatctta gaaaaggtga ctgcaagttc aaaaggttga
      901 ggcatctcca atctttcctt tcgcgtgttt tttgcttgag ccagcagatg cttctctctt
      961 ttgagtagca tctgaaaagg aattcttgct gctttcaaca tctcgcttac ttgattgaat
     1021 cctctttctg ttcgcctttt ttcagataaa ggtattggac aatagatgta tgtcgggtat
     1081 tcttttttta tctgcgtcca tttatatgca aaggtgcctg ctagtcggat atctcccata
     1141 aacttgtatt gattgagcca ggccttcata ttttcatcgt acacaaaaaa ggcacggtgc
     1201 tgaaaatcat aggtcggata tttcttcttc cattcttgac agtcactgca gtaaacggga
     1261 atagacttta ctttcttttt cgtactctct agattgaaat ggattggttt ttgacagcca
     1321 gaacagcctc ttgtttccaa taaatgaaat tgtgcgcgac aaactgaaca agtctgaggt
     1381 aatctgtatt tcgaaacacc aataatctcg ctgaatgtta aattttggac gatatcttta
     1441 ctgcagcaac tgcacctcac agcatcttct tcccttcttc attcatttct ttgatttcct
     1501 gacaagcttt tcgaatagct gcagtttttt cttcatagaa aaagtatact tgactgttcg
     1561 tataaggccc tttacgatcg gctcttcctg cgatttgtac caaagaagat ttagtaaaaa
     1621 ccggatgatc cgctcctaaa acgataacgg atattctttc aaatgtgact ccacgttcaa
     1681 ggatcatcgt ggttaacagt atgtcatatt ttgcttctcg cattttctgg actttttccg
     1741 cccgctgatt gtctcttgaa gaaacatctg tgatctcata tctcggaaga gcctcttgaa
     1801 gtgtggcttt caaacgatgc atcatcgtga tacttggaca aaataaaagg atatggtttt
     1861 ctttaattaa ttgatgaatg atccgcacca aagattttat cttcttcttg ttcttgcatt
     1921 tttgtcgcca attattcatg aaaaccaaga cgggttcagg aagtaaacgc cgatgaaagc
     1981 gcaaagcaag tttgtggatg tcaaacgtct gcttgatttc ctttagtaac tttttatcag
     2041 gtgtggcact taagtagatg aatcgtccgt ctggtttaat cgctgtttgc acagcatgag
     2101 ctaatcctgc atccccgtta taaggaaacg catcgatctc atcgatcact attaaatcaa
     2161 aggaccgata aaaacggtat agctgatgaa tcgtacagat aacaaaagaa gtaaaggtat
     2221 aagccgttgc tgtattccca tggagcaacg tgattttttc ttctggaaaa acataacgat
     2281 agcgtaaaaa caattcatta caaacatcga ctcgcggtgt acaaatcgct actcttcctc
     2341 cagcttttaa agtttccact aaaataggat aaagcatctc tgttttccct gcacctgtta
     2401 cagcccaaat aagatgatgt cttttttctg aatggcaaag tattctcttt gcgatttccg
     2461 tttgtttttc agtaagctgt cctgtccaaa aaaagacgac ttctcgaggt tttgcttcag
     2521 gctgatcaaa caaatataaa tcttgttgag tgtcgcatcg ccctaaatat agacaatgtg
     2581 tacaaaaata accgtaatct gcaagctgtc catcaatgac ggacgtcctc attccacagc
     2641 gatgacaaag aatccagttg cccaaacgtt ccactgctgc gatcttctca taatttggta
     2701 aatttacctc ctcacagtct attgctttaa tatctctcgt cagaagcctt ctaccccaaa
     2761 aatgattcat attattcgcc ctaaaaaaat atacgaaaaa acaagcgatc tattttttgg
     2821 atcgcttgtt cgatagttat actgttctat ttattcacct atgagcgtga ttgcttgatc
     2881 taacactttt tcgccattca tcataccata atcagccata ttgatgacgt ctaaaggaat
     2941 gccttttggt tccagacgtt tttcaaaatc agctttcata aagcgaactt gaggtcctaa
     3001 taacagtaca tctacatctt tatttgcaag attgttatct gcgtcagaag cggacacagc
     3061 gaaaatatcc gagtcgattc cttttgcttc agcagctttt tgcatttttg ttactagtaa
     3121 acttgtgctc ataccagccg aacatacaag catgattgtt tttttagcca tacttttctc
     3181 tcctttttaa aacaaactac gcaaacgttt tcttcgcgtc tgttactcta ttatataaaa
     3241 agaaagaaat ttgtcaacgt tttctaaacg cggtttattt aatcaaatag ccaactagat
     3301 ttcactaatt tattgaacac gacatattct ttgtccaaat tgatgacaac tacttcatag
     3361 cctgcattta tccagccata ggcacctttt tcagggtgtt tataatcatt tgcccaccaa
     3421 tctgtacttt cccttgctgt tttgggaagt ccgttactgg gaaacagcag ttcgtcaaat
     3481 tgggtaaagg tcaacgttac ttgagagccg ccattgttct tcaaataatg cgtaattgca
     3541 tcgtactttt tcattcgttc ctgcatcccc agatccacct acctttgtct ttattatcgc
     3601 atactacagc gattgcgcaa ccgttacagt gttgaaacaa cctagaactt tatttatact
     3661 gaataaagaa aatgaaaaaa agaggtgaca agatgatcga gcgctatcgt acaataaaag
     3721 aaaatggaca aagtgagatt gagatcaaaa aatcacgctt tatctgttct cttagacgaa
     3781 tagaatcaga ggaccaagca aaaagtttca ttcaagaaat aaaaaaagaa cattggaaag
     3841 cgaaccataa ctgcagcgcc ttcatcattg gagaaaaaaa tgaaatccag cgaagcagcg
     3901 atgatggaga accaagtgga acggctggcg taccgatgtt ggaagtcctc aaaaaaaatc
     3961 aactgatcaa tgtagctgca gtagtcactc gatattttgg aggaacgaaa ctcggcacag
     4021 gcggattaat acgagcatac actcatgctg tctctcaagc attgtctgac attggaatcg
     4081 tagaaggtac cttgcaacag gagatctctg tgatgatcac gtatccgcaa ctaggaaaat
     4141 tacagaatta tcttgaactc caacattttt ttataaaaga aatccaatat actgatcaag
     4201 ttagcgtcat ctgtgctgta gatgaaaaaa gtgctgacca ctttaaagaa tccatcattg
     4261 agctgctgaa cggacaagtc caatttgaag agggtcctgt ttcttatcac gagcaactgg
     4321 taactgatca tatataaaaa caggtgattc tgattctctt ttatttctaa gtaaggaatc
     4381 agggatcacc tgttttttat ttttccaaat aaaactcaaa acgatttcct acgtattgcg
     4441 tacgcacata ctcaaacggc gtaccgtctt caaaataaga aatctggcgc aaacgaagaa
     4501 ctgcatctcc tcgttttacc tctaaatagt cagcaatttt ctcagaagcc aacacagcag
     4561 taatcgtttg attcgctgga ccgatctttt tcccgccttt ttcttctagc gtgcgataca
     4621 acgattcagt aatctctgct ttgctgtagc catcgatcag cttttgtggc acactggcaa
     4681 cttcaaaaca aatagggaca tcatctgcga aacgaatacg ttccattcgc aagatcgtgt
     4741 catctttact caagtttagt ttttccattt cacttgagct tggcgaagtc aagaaataag
     4801 agattgtccg actagacggt actcgatttt gcgattccat aatttctgta aaacttgttg
     4861 caccagacat tttttcttgt actttttctc gcgcaacata tgtacccgaa ccgatctttc
     4921 tttctaagat tccttcatct gccaagtttt gaattgcttg tctcaacgtc atacggctga
     4981 cattgaattt cactgctaat tctctttctg aaggaagacg gtctccgact ttccatatac
     5041 cttgttcgat ttcatttttt aattggtcat gaatctgtat atatacaggt aattgattcg
     5101 acataacgta cacccgtcct ttcgtttctt ctattataac tggtatatac ctgattcaca
     5161 actgaaaaag ataaaggttt ctatccatca acagaggatt tagacacaca tcgttcttcc
     5221 tactcctatt gtcagaaaca attttttaga tatgttcaac ctagaaaata acaaaagacc
     5281 ctaaaatcga tgaaaatgcc gacttatgga tcttttgtta ttattagcgg tttttagcca
     5341 tccatttttt tgtatgattc gttgggcgtt ttgattttac ctggaccgct tttccgcctt
     5401 ttttcggaat attcacttca atattgtatt tttctgattt gaccaaatca tataactttt
     5461 cagctgcttc gtctactttt tctttccatt tcatgtggat ccctccaatt cttgtagtta
     5521 acttaactct atcttaaatc agcagaat
//
LOCUS       DACDYM010000115         5459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDYM010000115 DACDYM010000000
VERSION     DACDYM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.120"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..513
                     /locus_tag="IWM59_002524"
     CDS             <1..513
                     /locus_tag="IWM59_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP9980512.1"
                     /translation="TTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPV
                     TPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGW
                     TVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQ
                     WFAPDFAVSM"
     gene            complement(581..2380)
                     /locus_tag="IWM59_002525"
     CDS             complement(581..2380)
                     /locus_tag="IWM59_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9980513.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            2503..3009
                     /locus_tag="IWM59_002526"
     CDS             2503..3009
                     /locus_tag="IWM59_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9980514.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            3088..3810
                     /locus_tag="IWM59_002527"
     CDS             3088..3810
                     /locus_tag="IWM59_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9980515.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3888..5288)
                     /locus_tag="IWM59_002528"
     CDS             complement(3888..5288)
                     /locus_tag="IWM59_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9980516.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
BASE COUNT         1559 a         1041 c         1128 g         1731 t
ORIGIN      
        1 acaacagaag aatcaacaac acctgcgcct acaacaccat caacagatca aagtgttgat
       61 acaggaaacg gcacaggaag tagtactccg gctccaacgc caacaccaac acctgaacaa
      121 ccaaaacctg taacacctgc tccagctcct tctggaagtg tgaatggtgc agcaatcgta
      181 gcagaagctt acaaatatat tggtactcca tatgtttggg gcggtaaaga tccaagtggc
      241 tttgactgct cagggttcac acgctatgtt tacatgcaag taactggtcg tgacatcggt
      301 ggttggacag ttcctcaaga atcagctgga acaaaaattt cagtttctca agcaaaagct
      361 ggagacttgt tattctgggg gtcacaaggc ggaacttacc acgtagcgat tgctttaggc
      421 ggaggacaat atatccatgc tcctcaacca ggcgaaagcg taaaagttgg ttcagttcaa
      481 tggtttgccc ctgactttgc tgtcagcatg taagaatgat taatcaaaaa gagttagccc
      541 attctggact aactcttttt ttattgtgat aaaatagaaa tcagataaat tcttctgtca
      601 attccatgaa cgtttggata tcttcagtca ccatgtcgat acctgcttgc caaaaatctg
      661 gttgggtaag atcgacattc aaatgtttct tcgcaagatc ttccgtcgtc attgaggcag
      721 tatcgcgtag taaatggata tattcttctt caaaatttgc tcccttttgg ttggcgtaag
      781 catagatgcc caaactaaag aggtagccaa atgtgtatgg gaagttataa aacggtacgt
      841 catcaataaa gaaatgcaat tttgcagccc agaaatatgg atggtaagta gctaaagctc
      901 cttgatagcc ttctttttgc gcctcaacca tcatctctgt gatcttatct tctgagacta
      961 atccttcttg ccgtgcttca tagaatcgat tttcaaaaat gaatcgggaa tggatattca
     1021 tgaacatagc taaagcattt tgcagttttg tatctagcaa attgattttt tcttcttttg
     1081 tttttgcgac ttttaatgta gcatcggcta cgatcagttc tgcgaatgta cttgctgttt
     1141 ctgcgacatt catggcatat tcgcgattta aagaaggtaa atcccacatc gtgctgctat
     1201 ggaaagcatg tcctagttca tgagcaagtg tcgcaacctc attaacagaa ttactgtagg
     1261 tcataaagat ccttgattct tttgtttctg gtaactcagt acaataacca ccaggacgtt
     1321 tcccaggacg atcttctgct tcgatccagc tcttttcaaa agcagattgg gcaaatttag
     1381 ccatttttgg actgaatttg ttaaagtttt cgataataaa tgctgctgct tcgtcaaaac
     1441 taaaggtttt ttctttcaaa tcgcctaaaa taataggtgc atcttgatct tgccattcca
     1501 ttttttcttt accgaaaaga ttagcttttc tagtcaagta attaacaagg ggttgtttgt
     1561 ttttctggat tgtatcccac atgacagaca atgtttcttt cttcaaccga ttgtactcta
     1621 atggtttttg tagaaaatct gtgacaccat gaagttcata gtcagaaagt ctaaaaccat
     1681 ctaaatgatt caaagtatcc gtgaagaggg gtgccttttc tttccaggct tcttcccatg
     1741 ctgcgaaaag tgtttctctg acttttggat ctggatcgcc catcatctta ttgaaggctt
     1801 gtccagcaga taattcgact acttgtccat cttgttcgaa gggaatcgaa atgctggcta
     1861 caatcgtatc ataatgactg ctccaagcat ttagaccatc taaagaaagc gtatttataa
     1921 tgttttcttc agcttctgat aataattgtg agccatcacg acgaatctcg tttaaacgaa
     1981 aagcaattgt ttcaaacgaa gattgagaaa gcatctgatt ccaatcattg tcagatattt
     2041 ctgcaaattt ctttgacaat acagtttcgg ctgattgcag acgaggcagc attgcgtaaa
     2101 gcttgcctga taagaccttt gcatctgaat cattgacatt ggcagaaagc aatgcagtaa
     2161 tatagctgct gcattgggta aaaccattcg taatcgtttc ttgcaattga agaatagcgt
     2221 ttaactgctc tgcattgtca gaactaggtg accattttgt tacgcggtga taatactcgt
     2281 tcatttggtc ttccaattgc ttcatccgtt ggttcaaagc atcagaatga ctgcctccgg
     2341 aaaaaataga atctagattc caatttaatg aataactcat gaaaaaactc ccttcatatt
     2401 gtaatacaaa attaagtata gcactttttt atggaagaag tctctatgaa aatgattgtt
     2461 gtttcttcaa aaaaagtcta agataaaata cagaggtgac ttatgaattc ttcaaaaaaa
     2521 caaatgaaaa acggaaatat ttatttagta atcgcactga tagtcgttat aatcttattt
     2581 tacagttctt ctcaaactta cggacagcaa tcacaagttt cgcgattaga aagctggctg
     2641 cctaatcagc catttaaaga cacattagcg ggtatacgtt ttaattatgg tggaagtatg
     2701 gtgagtatcg accatctggg ctacgccaaa tttttagagt tctttttaag gaagggcgca
     2761 catttcggta cctattttat tttgggaggc agcttgtttt taggtgtttt ccccaagcta
     2821 aaaatttggt ggctgacagc gatccttgct tggctttcag caacagggta cgcaggtttg
     2881 gacgaattcc atcagatgat gaccggtgga cgaacaccta tgttccaaga tgtcatgttg
     2941 gattcgatcg gtgctctgac tgctgtgata ttatgtgtgc tttttgcggc attcaagaag
     3001 cgtttataga ttggctgtgg ctttgttttt ttgtgaaata gtactagaat agaagtgtat
     3061 tctcaaaaga acaagggagg aaaaatcatg tcaggacata gtaaatggca taatatccaa
     3121 ggaagaaaaa atgcccaaga tgcaaaacga gggaaagtct ttcagaaact ttctcgtgaa
     3181 atttatatgg cagctaaagc aggcggtcct gatccatcta tgaatccagc tttgcggctt
     3241 gttgtggaca aagcaaaagc tgcaaatatg cctaacgaca atgtggaacg ggcgattaaa
     3301 aaggctacga gtgccgtaga tgaaacgaat tacgatgaaa tcacgtatga aggatatggt
     3361 ccgggtggag taggaatctt agtccatgca ttgacagata accgtaatcg gacagcgaca
     3421 aatgtccgag tagcttttac tcgaaacggt ggatcactgg gagaaacggg ttcagtcagt
     3481 tacttgtttg accgcaaagg atatatcgct attgaacgcg atgggttgac tgttgatgaa
     3541 gagacgatgt ttgaggatgt attagaggcg ggggcagaag atctgcaaac ttctcctgaa
     3601 gtattcgaga tttatactgc accagaagat tttgctgctg tacgagatga gttggaaaaa
     3661 gagttcactt tggcgcaagc agaattgacg atgattccac aaacgacgat tgatttaaat
     3721 gaggaacaaa aagagcaatt ggaacgtcta gtagataagc tagaagacga tgatgatgtt
     3781 tcagaagtct ttactgctgc tgaaatgtaa gtagcacatt gttagataat aaaaagaaag
     3841 cacctgtacc aatcgtacgg gtgctttctt tttatatatt atttttgtta gaagaccaat
     3901 acaggcgttc ctttttcgac catcccgaat aattccttca ttacacttgg tggggtgttg
     3961 atgcagccgt gtgaaccgcg ggttttccat aagtctccac cgtattcagg ttgccagtct
     4021 gaatcgtgga taccaacacc tgtccagtca atcggcatcc agtagtttac cggactctcg
     4081 taaggtgttc catcgtcatt tgttcctttt aaagttgcat cttcttcttt attccaaaca
     4141 tagaagactc cagcaggtgt aggtgtagtt ggctttccgg aaacgatatc tgtttcaagg
     4201 gccacttttc cgtctttgta ataccacata tgctggttct ccagatcaac ttcgatatac
     4261 gtatcttcga tcaatggatg atcagcggtt gtgccacctt gcacaatggg tgaacgagta
     4321 aaatcttggc ctgccagaat cgcttttttc aatgcttctg tctcactgtc tgtttggatc
     4381 gtccaactgt aagtgccaac tgggaccgta acttctcctc tttttgtact tttaaatttt
     4441 gtgtcattcg tacttgtgtt atactttgtg cctaaatctg ttacatattg acgtacttgt
     4501 tctgtatcta aatcgacttt tccatcattg taggtcagcc aactcataat atcacttgat
     4561 gggatctgga aagtctcacc gtttatactg taagtagctt ttacatttgc gatagcattc
     4621 atggatgcga gttgttcttt cagtgagctg tcttcagatg ttacttttgg tttttctttg
     4681 aattcagtca attcaattgt atctttacca gagtttaccg cactttttaa ttgtttgacc
     4741 gcagcatcga cgtcgatcgt atttccattg acttcaggct tgataacgaa agagtcacct
     4801 gctttttcga tcgttgcatc agtagtaggt gtccggtctt tgttcaaatc agtcagctta
     4861 gttgtcaatg tctgaacggt agtgtctagt ttttggtggt tttgactgac aggatctatt
     4921 tcttgttttt ctgccgctga gacataagtc attccccaag tccatccgtt ttgttcattt
     4981 aggatcggag aaagctccga agtgaaatca tctttgtagc cgagctcgga ttttggtact
     5041 tcctgccaga cttttccatc ttctttaata gccagttttt tatcagaata agcttctttt
     5101 aatttttcat ttgctttttc gactgttaga ttgctgacat ttattccatt tatttctgta
     5161 ttcggcaaaa aatgctttgt gtagtatgta ctgcgatagg tataaaagct gatcagcact
     5221 aagagaacac caagaactga aagcaaagca acatgtttcg atttttttcg atgcgaagat
     5281 cttgtcatat cgacaactcc ttaagtaaaa gtttagggtg gaacggtctc atgatagcat
     5341 aatttagagg aaaagacgaa ggttatctct actttatgaa aaaatttaga aaccataagg
     5401 gtgataaaat aaaaaaacgg aaaacaccga atagatgttt tccgtcatgt tcaaagatt
//
LOCUS       DACDYM010000116         5441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDYM010000116 DACDYM010000000
VERSION     DACDYM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.121"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1614)
                     /locus_tag="IWM59_002529"
     CDS             complement(<1..1614)
                     /locus_tag="IWM59_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9980517.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFK"
     gene            complement(1777..2370)
                     /locus_tag="IWM59_002530"
     CDS             complement(1777..2370)
                     /locus_tag="IWM59_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980518.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(2371..2703)
                     /locus_tag="IWM59_002531"
     CDS             complement(2371..2703)
                     /locus_tag="IWM59_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980519.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(2753..5284)
                     /locus_tag="IWM59_002532"
     CDS             complement(2753..5284)
                     /locus_tag="IWM59_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980520.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            complement(5281..5418)
                     /locus_tag="IWM59_002533"
     CDS             complement(5281..5418)
                     /locus_tag="IWM59_002533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980521.1"
                     /translation="MLIQFYEEQAADRLQEYVEKLIKNEEISEMIGKRLLLESEKVVK
                     E"
BASE COUNT         1457 a         1143 c          875 g         1966 t
ORIGIN      
        1 tttgaagtct tgttcttcca tttgtttttc tttaattaaa cgtcgttgtc ttcttccttg
       61 aatcaatgcc tgattccaaa tcaacgtcat agccatgtac atttgacatt gttgtacccc
      121 tcgatctaag cctgtaatag agtcaatgtc aaaaaagacc aactgttgtt ggtcaaggtg
      181 tcgaatcgta ctatgaccat caaaaatcat gccatacgtc tcacataagg attctacgat
      241 ggattccatt tcttcttttc gattcttttt ctcttgggaa gcacctggta attccagttg
      301 tcctaaaaag tctaaaaatt ctttgaacgt cggataagct tctggatctt tacctgtgac
      361 ttgcgtatca gtatttgaac gcgaatttga ggaagtaaag ccgcgtggta ctgttccata
      421 ctcttcataa aaacgattta aataggtctt aaattctgct ttcatcgttc cttttagctg
      481 aggactcact aactgtacct gatagaccac tttgtctaag tgcgtttgat aggacgctgc
      541 ttcattgatc ttcaaatctt tgactgatcc atcgtcatat aaactggaag cttcatcata
      601 cgtacgtgtg gcaaatattt ctaaaggatt gatcatcccg tcagatccgt ctaaacgcac
      661 cataatgccg ttatattcct ttaaaaaggg aataaagtct tttccctttt caaagcccca
      721 aataaagtct cctgcagcta aatgggcttc cgcgatcatt ttaagaacgg tcgatttacc
      781 agctcccatc attcctagaa gcaaggtatt ggtagacaac cgcacactat ttttatacgt
      841 ggcattccaa acaaacgggc cgcctgtcat ggtttcccca tattgtgttc cacctggatc
      901 ttttagcgtt tggtggtgga acgggaaacc tccgccaatg tttagcgatg gtagtgattg
      961 ccctttgata tttccaaacc attcagcttg ttcatcgaac atggtgaaca tcgattgcca
     1021 gtctcttttc gttttgtgca gataggtcac tgcacgataa tccatccctt tgagattttc
     1081 gatcagttcc tgtgctcgtt catctacttc ttgaatcgtg tttccgtaaa catgtactct
     1141 cactttgact tgtttagaaa tttccccatt agaagtcaat tttcgggcat attccgctag
     1201 ttctaggtat tcttccgcgc ttttatttcg gtcagtcgct tttcgttcat tatctgaccg
     1261 atcatttaat tcatctaagg cacgattgat ttgatcaatc actttttgct tttcatctgt
     1321 gctaacatct agtgttgcca ctgtatgagg attgttcatg agattggcca accaaaaaag
     1381 cgtaggattt tttgtgtagt gataaactgt cacacatcgt gaataaccat ttccataggt
     1441 cacatactct tcatcgaaat ttaatccacc ttgtggcatc acttgtttta aaaagtctaa
     1501 atcataccct ttggatttta aaatttctat ttcccgattt cctagttgct ttaatggatc
     1561 aagtaccttt tgatcatttt gtggtgaaaa agctgtttta attgcttcta acatgtacag
     1621 tcacctcttc ttgtttgatt ctttacttgg ttaaaagatt ttccttttcc caacttaccc
     1681 aggcttaaag tgtgaggaaa ttttctagtg gtctcttctt tcggaacatc tttttctaac
     1741 catatcaacg gatatttcat tctctcttcc ccattcctac tcatcattca tattattcat
     1801 tcgataaaga atcttgatca attctttttt gtagagtggt tttaacccta aaatgccact
     1861 agatcgcatt aactgacggt ttctggtttg caaatccttg attgtctttc cttcaatgac
     1921 gaaaaagaag cttaaatcag ggcgttcctg ttctgctcgt ttcaattcct ggatttcctc
     1981 tactaatttc ttttcatatt ttcttaatcg ctgtagattt cttcctaagg actgttccat
     2041 acgattttta tctaataact gccgcttttg cacgatttgt tccgttagat ccgtctctgt
     2101 ggtcaaacta actaacttaa ttgttggcac atacgtacgc atgacggtgg tcaactgatt
     2161 cattcgttct agtttctctt gttcatttaa tccttccacg tctgtagaaa ctacacgata
     2221 ataacgttga aaatgatctt ctctagtaat taagactccc tcttctgtac aggatttata
     2281 cggaaataag tccaagatag agcgctcttc tggttttcca aactgcaaaa tggatttcct
     2341 ttcaactgaa ttttgtcctt tgactcgcat ttattcatcc tccttatacg taccatgata
     2401 atttcggtta ggatctaacg catgataatg gttcttgtcc gcaaaaaaaa tagactcgat
     2461 aatcgcaaaa atttgtcgtt gacctgggtt attatagggt cgccaaacaa tcactaacgc
     2521 tatcgcaatc cataagccat aattcaagag tgcaaaaaag agtggtaagt ggacaagatt
     2581 attgaccatt ttcgcccaaa aaaagcagcc accaagcgtt aatagatcta aaaagaaaaa
     2641 agcgaaaaac ttccattctc cactaaatct tggagggttt ttcatgttta tatatttatc
     2701 cataattgat ttctcctttt catataaaga aaaggcctaa cttgtaggcc ttttatggtt
     2761 tgatatcctg tttgatatcc ctcaatagtt gttctttcaa tgcattgttc ctgtttttag
     2821 tttctcttgt cacatttatt tttgttggtg cagtaaatgc cacatttgct ggcttgtaac
     2881 ctgtatgaac gttttgaata ggttttggtg cagcttctct tgtcacaaca ttttgaacgg
     2941 acgcattccc tgtctcacca ctttcagata aattgtaaga aacagttgtc agatttccat
     3001 tttcaggcaa acgacttggt ttcatttgaa cagtacctgc aggtgattga tgaacaaaac
     3061 tttgatcatt atccttgaat tgattcaaac gactatcttt cattggtaaa ttttgtgcct
     3121 taggtaaacc aattttcgtt gatttttcag ttccaacttc gattccatta tcatttggca
     3181 gaacgatgga atcctcatcc gtttgtactt cttgatttcg ttttggtaaa tctgaaatgc
     3241 gtttttcatt ttcggagtta acagatctat accgatattg ttctggatca gtttttggta
     3301 actgtccctc attttctgct ttgtcttctt gatttacttt tgataaatct gaaattcgct
     3361 tctcattttc aaggtcactg ttaggtttat agcgatattg ttctggatca gtttttggta
     3421 actgtccatt ttttccttct tctgaatttc tttcttctat ggaatttttc tcttcaatcg
     3481 aattttcatt agagtgcaac gcttcttggt gatctttgac tgaaggaatc actatacctt
     3541 cttgattttc tttttcggtt gttgtattag ctttcactgc tgcattgact tgtccttcac
     3601 catacgcatc ttttagtcct tctttgactt ctgaagcttt ttctttttgt ttttctagtg
     3661 cttcggatgg attcttcaca gcttcagcca attctttagc tctcgtgccg tctaatttat
     3721 cttctaaggt gtctttggct ttttctgtaa gatcttcagc tgttcctttt aacccacgtt
     3781 cattgacgta accaagacct tctgcggctt tagaaacacc atttttcgct ttcccgaaca
     3841 tttcggaaac tgttttctta ctattaggta cagttgtagc tttatcgtct gattgttctt
     3901 ccccgttatc aggggtaaca tcttttcgat ggccaaatcc atttttacga atatcattta
     3961 aatggcttaa accactctta ccagcacttg caaggttttt agctccgcta cccacatttt
     4021 tcgcaatatt tcctaaggca aacatgctaa gaagagaatt actgccgttt ttcagactcg
     4081 tatcaacgcc gaaaattttc gtaactgatt gagagccttt gaatagcgca atggttagta
     4141 caatcaaagc gacaacatac aagaatccat tgatctttcc tgccgcatgc aaatcaccaa
     4201 tacctgaaag cattaaggta tagattcgta attctaaaat tgtaaatgcc aataacaaga
     4261 aggattgaga aatatcttcg atagcacttt tcatccgttg tcctgtctca aagtccgttg
     4321 acgcggcaat cactaaatag aattttttaa ataccagctc aataatgcaa gtaacgatcg
     4381 taaataggat aaataaatag gccaccgcca aagaagattc accagctaaa ataatccctg
     4441 gactactaat gtatcgacga taccctgaag tataaacata tttggctaag ccttcatcac
     4501 tgatttgcac aggaatttct tttccattat catccagttc cagtcggtat tgtaacgctc
     4561 ttaaccgatc cttttgtaaa tctgataaac cactcttatc tttatccgcc atttctttag
     4621 cttgtttagg agtaataatc ccattgatat tcgctgtttt aaaatttttc gctgttaaag
     4681 catttctttt gtctgttggc agggattcta ctttattcgg gtcagttgct agtaatgtac
     4741 tgacatcgat cacattattt tgaataattt gaaaagcagg tgaacccgtt ttatctgctt
     4801 tatttgctgc agtgacgtct ccgtaaaagc ttttactgat atttaaccct tcatcgatta
     4861 aagaactacc accaaaaatc aagatcatgg ccataaataa attaaccaag atatttttga
     4921 cttttggtgg gtgtttactc ataaaaaatt tcaagcctaa ataaattaat gtgacgacac
     4981 aaaaaagagc caatacctta ctaatcaaac tttgaaatag ttcatttacc ccagcggcgt
     5041 tcaataagtc ttttaaatca aagctttgat aaatggtttt ttctagtaaa gtattgagat
     5101 tatataaccc tttgatcaac ccccataaaa aagtattaca aatccaactg aacacatctt
     5161 gatactgcag ataattacca taatcttgta agactttcgt tacggttttg gggtttgcat
     5221 ccccttctcc gaataagcct gaccaccagt cggctatgcc agaaaaaatt cctccaatga
     5281 tcattctttc accacctttt cagattccaa taacaaccgt ttcccaatca tttcagaaat
     5341 ttcttcattt ttgatcaact tttcaacata ttcttgaagt cgatctgctg cttgttcttc
     5401 gtaaaattga attaacaatt ttttagccgt ctcattctca a
//
LOCUS       DACDYM010000117         5264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDYM010000117 DACDYM010000000
VERSION     DACDYM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.122"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(224..1186)
                     /locus_tag="IWM59_002534"
     CDS             complement(224..1186)
                     /locus_tag="IWM59_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9980522.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1188..2627)
                     /locus_tag="IWM59_002535"
     CDS             complement(1188..2627)
                     /locus_tag="IWM59_002535"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9980523.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2812..4758
                     /locus_tag="IWM59_002536"
     CDS             2812..4758
                     /locus_tag="IWM59_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9980524.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANIAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVYSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5022..>5264
                     /locus_tag="IWM59_002537"
     CDS             5022..>5264
                     /locus_tag="IWM59_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9980525.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1701 a          890 c          873 g         1800 t
ORIGIN      
        1 ttaaactggc aaagtcccgt ccaatttcgt aaaacaaccg taacagttgt gtaaaaatat
       61 cattttagag catgaaaaaa ggagtggatt tctccactcc aaaaaagtct aacttttggg
      121 ggtcactaca agttgacaac tcctttttgt ttacaaatca attggatatc tacaaattgt
      181 attttctaac gaattttata gacattaaaa aattaattta acgttaaata cttcctccag
      241 aaagtattga gattggtaaa atataatcct tatttgtctt ttcgccttta atttttttca
      301 gaagaatatc aacaataaga cttgcaattt catcaattgg ttgtcttatt gttgtcaatt
      361 gtgggaaaaa tttctcaata aaagaggtac catcgtatcc aataattttt aaatcttctg
      421 ggatattaag tttaagttga tgagcaattt tcatagtgag aatggctgtt aaatcatcag
      481 aaacaaaaat accatctggt ttgtgattta taatagttga ctttatttcc atttcacgac
      541 ggatagtaga cagattatta ggaattttga aaatttttcc atttggattt tgaaaagaaa
      601 aacctagtgc acgtaattct gtgggagaat ccgtgttatc atttcctgta atcataatgg
      661 tattttgaca accatttttt tgtaaagtct tagctgcaag ttttccccct tcaaagttat
      721 ctgaagaaac aatggaaaca ttaggagcta aatttctatc aaaagcaaca ataggtgctc
      781 tcacacgctc ataatcagca attccaagat tatggcttga tgagattatg ccatcaactt
      841 ggttggcttc aagcatttca atgtattctc tctctttagc aggatcattt tgactattgc
      901 aaataatcgt tttatatcca tgtttaaata actctatttc gagatactca atgagttcag
      961 agtaaaagat attactaata ttaggaaaaa taagaccaat aagctgaggt gactttcctt
     1021 gaagtccacg tgctaaatta tttggtttat aaccaagagt tttcatagct tcatgaactt
     1081 tttgttttgt tttttctgat aaatatccct tattattaat gacgcgtgaa acggttgttg
     1141 ggctaactcc agctaattca gcgacatcag ttaattttgc taccatttta atattttaat
     1201 tcaaagtatt ttccagtagg ttcgcctgat ttgatttgaa taccattttg gtgattttca
     1261 gggaatacac gactagtaaa cactttttct cctttattaa taaaaatctc aatgattgag
     1321 ttatctacaa aaatgttagc agttgtttct ttagtatcaa ttgagcattc acgaatcgta
     1381 ccaaagtcag tggcatattg aatgcctgct ttgctgcgat ctaaaataat ttttccttct
     1441 ttggtatcta caatcaagct aagtccattt cctttactat cagagaaaag aagtaactcc
     1501 gatttctgat ttggaggaaa agttagttca agctcataag tattatttgt ttctgctttt
     1561 gctgtaaatt gttcagaact agcacgtaat gatgtaatag cttctactgg atattggtaa
     1621 agtttaccat cttttattga aagttctttt acaagactca ttgctccctg ataatcatac
     1681 ttatcagttg gataatcgat atcaggtagt ccaatccaac tgacaatcag agcacgacca
     1741 tcaggagtat tgaatccttg agtcgcatac gcttcaaatc cataatcaag attttgaatt
     1801 tctgaagcgc ccacaagttt tactttttct atgtcaagcg attgacagat cttaaaagta
     1861 ttcggataga tattcccata atttagctca gatttatcca acccttgagg acaataaatg
     1921 agaacaggtt ttttgtctac aaatactaga tttggacact caatcatgta ttctgaacca
     1981 gtgccgccaa aatcaaggtt gccaacttcc tcccatgttt ccacgttatt gtcatccgct
     2041 ttgtaaagtt tgataaagcc tgatttttct aaattttgtg ctccaacaat cgcatataat
     2101 tgtccttcat aattaaacat ttgtggatca cgaaaatgat cagttacatc agcaggttta
     2161 tgaataagaa cattctcacc tttcgtaatg ttgtagtctt tatccatcca tgctccgatt
     2221 tgaagtgtat cacgttccca attttcattt cgaacgtttc ctgtatagag taagaataga
     2281 ttcccctcta tttcatatgc agaaccggaa taagcgccgt ggctatctcg ttttgtgtct
     2341 ggaagaagtt ctgctcctgt tacatgaaaa tgaactaaat catctgattc attgtgtacc
     2401 cattctttca acccatgagc agctccaaat ggccaatttt gataaaaaag cgtatatttt
     2461 ccgttaaaaa aagaaaaacc attaggatcg tttaacaatc ctgttttagg ttcgatatga
     2521 tagctagcac gccaaggtga tcgttttaca ttatccttaa ttttatccat ctcatcttta
     2581 gtccaatcag aataagcacg atatcgtatt tctcgaggta agttcatatt ttactccttc
     2641 aatttatctt tttacaacta gtatggtaaa cgttttccgt tgaaaaatca atcatttcat
     2701 ttaaaaaaat aaaaaatatg acaaacgttt gacatatttt taagatatgc taaaataaat
     2761 ttgcatttct gtgaaccgtt ttcacgtaaa aataaaaaag gagtgattcg aatgaattac
     2821 agtaaagtag cttccgacgt aatacaagcg gtcggaaaag ataaccttat tgcagctgca
     2881 cactgcgcta cacgtcttcg tctcgtacta aaagatgaca cgaaagtgaa tcaaaaagct
     2941 ttagacgaaa atccagatgt gaaaggcaca tttaaaattg atggacaata tcaagtgatt
     3001 atcggtgcag gtgatgttaa tttcgtttat gacgaactta tcaaaaaaac tggactttcg
     3061 gaaatttcaa cagatgattt aaaacaaatt gctagtaaag ataaaaaatt caaccccatt
     3121 atggcattaa ttaaattatt atcagatatt tttgttccaa ttatacctgc ccttgttgca
     3181 ggtggtttat taatggcatt acaaaacttt ttaacagctg ctggactttt tggaccaaaa
     3241 tcaattgaag aaatgtatcc tgcgattgaa ggtatttcct caatgatcca actgatgtcc
     3301 gctgcaccat tcatgttctt accaatttta gtaggtattt cagctgctaa acgttttgga
     3361 gcaaaccaat ttcttggtgc tgcaattggt atgatcatga caacaccaga tcttggtggc
     3421 tcaactgaat attggaatat ttttggttat catgtttcac aaacaaacta tgcttatcaa
     3481 gttattccag ttttagcagc tgtttggata ttatctatat tagaaaaatt ctttcataaa
     3541 aaattgccat catctattga ctttacattc acgccattat tatcagtaat gattactgga
     3601 tttttgactt tcacagttat tggccctgta atgttattgt tgtctaatgg aattacagac
     3661 attatcgttt ggctatataa cactactgga tttattggaa tgggtatttt tgggggaaca
     3721 tattcactta tagtcatgac tggtttacat caatcatttc cagctattga aactcaatta
     3781 ctttcagcgt ggacaaatgg tatcggacat ggagacttta tctttgtagt tgcatctatg
     3841 gcaaacattg ctcaaggtgc tgcaacattt gccatttggt tcttaactaa aaattctaag
     3901 acaaaaagtc ttgcttcctc tgcgggtgtc tcagcacttc ttggaattac agagcctgcc
     3961 ttgttcggag ttaacttaaa ataccgcttt ccattctttt gtgctttaat tggttcagga
     4021 attgctacag cagtagcagg attacttaaa gtagttgctg tttcacttgg ttcagcaggt
     4081 ttcttgggat ttctttcaat taatgcaaca tctattccat tttacgtatt gtgtgaattg
     4141 attagctttg tagttgcttt tgcaattact tacttctacg gtaaaacaaa agctgctggt
     4201 atctttaaag cagaagaaat tgctgaacaa acaccagtta attcttcaaa agtagcaaca
     4261 acagatccaa tatctactga tgttgaatca acacaaacaa aagtttcatc agaaacaatt
     4321 gttagtccca tttctggtaa agtgattagt ctttcttcag taaatgatcc agtcttttca
     4381 tcagaagcta tgggaaaagg gattgccatt aaaccaaatg gaaacacggt ctattctcca
     4441 gtagatggta ctattcaact cacgtttgaa actggacatg cttatggtat taaatcaaat
     4501 aatggtgctg aagttctaat tcatattggt attgatactg tatcaatggg aggaaaagga
     4561 tttacacaaa aagttactac taatcaaaaa gttagaaaag gcgatattct tggagcattt
     4621 gaccgttcaa tgattgagaa agaaggtctt gatgatacaa caatgattat tgtgaccaat
     4681 acttcggatt attcagaagt aaatatagta gctgaaaata cagtcactga gggtactgat
     4741 ttacttgaaa tcaaataaat gtatttccct gagtaaagat ggaattttct ttccaaaatc
     4801 aaataatata gattaatcaa atatttgaga ataacatttt atttaatctt ttcatatatc
     4861 aattgtatga aaaattatta gaattttaaa aaatgataaa aattcaaaaa gaaaaaatag
     4921 tagttattta cttgtcatgt tactaaataa aaaaataatt taaatatgat atatagtcat
     4981 ttatttagtg atttcattct aaaataaagg gagaaatgtc tatgctatac ggtgcaattg
     5041 aagcaggcgg cacaaaattt gtgtgtgcca ttggcgatga agaaatgacg atcaaggaaa
     5101 gagtgagctt tccgacaacg acacctgagg aaacaatgcc tttagtcatt gacttcttca
     5161 aacaatacca agcagattta gcagggattg ggattggctc atttgggcct atcgatatcc
     5221 atcgcgattc cgccacttat gggtacatta cctcgacccc gaaa
//
LOCUS       DACDYM010000118         5205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDYM010000118 DACDYM010000000
VERSION     DACDYM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.123"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(67..1248)
                     /locus_tag="IWM59_002538"
     CDS             complement(67..1248)
                     /locus_tag="IWM59_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9980526.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            complement(1523..1827)
                     /locus_tag="IWM59_002539"
                     /pseudo
     CDS             complement(1523..1827)
                     /locus_tag="IWM59_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(1911..2381)
                     /locus_tag="IWM59_002540"
     CDS             complement(1911..2381)
                     /locus_tag="IWM59_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9980527.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            complement(2398..3330)
                     /locus_tag="IWM59_002541"
     CDS             complement(2398..3330)
                     /locus_tag="IWM59_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9980528.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            3976..4062
                     /locus_tag="IWM59_002542"
     CDS             3976..4062
                     /locus_tag="IWM59_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9980529.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            complement(4182..4871)
                     /locus_tag="IWM59_002543"
     CDS             complement(4182..4871)
                     /locus_tag="IWM59_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980530.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4873..5151)
                     /locus_tag="IWM59_002544"
     CDS             complement(4873..5151)
                     /locus_tag="IWM59_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980531.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDL
                     QVKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
BASE COUNT         1677 a          840 c          780 g         1908 t
ORIGIN      
        1 attaacttta aggtatcata atttttattg aaatagacat tctattcata aagtgttatt
       61 ttgaattcat ttttgtgttc cttttttttc gttagtatat ctactacaac acaacataaa
      121 tcccaacccc aaaaaattac taagaatggc agaaccacct tgactaatat aggggagtgg
      181 aataccagtt agaggtagta atccgatact tgcacctata ttttcaaaaa catgaaaaaa
      241 aatcattgca acaatgccta taccgatata ggtgaaaaat aagttattgc ttttaaaaca
      301 tgttttatac atttgataaa ttaaataaaa gtataggaat attacaagac aacttcctat
      361 aaagccaaaa ttttctccaa ttacagaaaa tatcatatcg gattctctaa caggaacgtg
      421 aatgtttgat acattaaaac cagtacctaa aagtccgcca gagccaatag caattaaact
      481 atttgctaac tgatagctag atccatttgg atctaaaaat ggattcaacc atgagtctat
      541 tcttttaaac tgatagtcgt gaaatcctat ttttaataag aaatctcttc ctgtatctga
      601 tataaccaaa tataaaaata ttaaggataa aacaacacca atggcgatta ttggggcaat
      661 tatttttata tttatgttag acgtaaacaa aataaaaata taaataacaa tgaagactaa
      721 cattgttcct agatcgttct gaaaaaataa taatattcct attggaagag tagcaataaa
      781 agttttaagt aaataaatcc aatcatcttt cgtattacta gtaatagatt tttgctgata
      841 attgacggtt attttagcta gcataagggt gtatcctatt ttgactaact cagtaggttg
      901 aaacgaaaaa ccagcaatat aaaaccaatt ttttgctcca gttaagactg ctaattgtgg
      961 attatagaat ttcaagagaa gtaataacat tatgatgcat aatccgtaaa atataggagt
     1021 taatttccac aaaattttag gattaaaaaa ataaatagtt attgctgaag tgaacccaat
     1081 actataccac aatacttgtt taattaattc ttttgtgatt tttgaattag tgtaagagtc
     1141 gtgatataaa gcaacatata aagaaagtaa tccaatgata catagcagaa aaactggcaa
     1201 taaaagccca taattcaact gatttttgta gattctatta tttttcattt cgaaaggtcc
     1261 tttttcataa tttataaata aaaataacac aataaagtta tttttactag ttttgagttt
     1321 tagacgaaaa ttacaattag ctactttttt atcttactaa atagttctat ctttttatat
     1381 aaagtataga aaaattaata aaacaattgg aaatatgctg tgaagaaata agtttctatg
     1441 tttattaaag ataataatta aagtattaat tattaatagt aaacttagaa aaaatattaa
     1501 taaccaagtg gcaattgaat ggctaaaata aaagaatccg gactgaatta aggtgaaaac
     1561 taaacttaaa aaaattacaa aatgaaataa tagtattagt gtttttgtat gattttttac
     1621 cataattttt tcttctccta acaaaatatt gtctagtgat aaatcaaata acttagcaaa
     1681 ctgaattaaa ttttctgtat ctggtattgt tcgaccattt tcccaattag aaatggtttt
     1741 tttagatact aaaagcattt cagctaaatc ttcttgagtt agttgttttt ctattctttt
     1801 ttgtttgatt actttactta tagtcatttg atcacctaaa catatttttt attcttagaa
     1861 agatagagtc tatagaaatt gatttcttct ttatggaaag ttggtaattg ttaccaaaat
     1921 ttccaccact ttttttctgt tgttattgca ttgttgtttt ctttattgac agaagttaat
     1981 tcctcttctt caggttcttc cgtataagct aattgtaact gttcttgcag tttttcgatc
     2041 tgttgatttg cctgcaaggt gagttgttgt tgctggtcga gtaacttttg catttgtttt
     2101 atttgagtat ctttttcttg gagctgttca tgtaaaaaaa caataatcga gttttcatcg
     2161 ttactagcaa cggtttctac ttggttacca gtggtgttac tacctaaaaa atgttgtttt
     2221 aaaagcaaat atcctttgta gctgacaact aaggttttca ctccgttcct agtaacagtt
     2281 tgcacatagt tttctctaaa atcagctgat agccttttcc taattgcttg ttttgaaaca
     2341 tgaaattctt ccgcgagttc tttgatcgtt ttatctatca taaatactcc ttatgcttca
     2401 atcgaataag ttttgtaagt tttctaataa atcttgttta agctcatttg agattgattg
     2461 tttttccgct tcttcttttt cggtttgttg ttgtttttta aaatcagctt catgagtacc
     2521 taacggaaga tttagtattc gatctttagc acgccatttt tctttttgtg ttaattctcc
     2581 attgtgttca atattaaata attttatacg taaatcttct tgcttccctt ttgaaaaatc
     2641 gtcagcatgg ttgatttcag ctttccaagt aaaacgataa ccaataacag gtttacctcg
     2701 acctttacca tattttttac gaatagttaa tcctttaaat agtggagtta gttcttcttt
     2761 tattggtttg agtacaaact gatttatatt tgtttgtcta tagcttttag gaatatctaa
     2821 tagctctaaa aaatcttcct tagaaaattc agcccatcct tgagttcggt attgttttag
     2881 caatctaaac attgttttag cataactgct atttaaatct cgaaattctg ttaaagagaa
     2941 acgtacccag ctttctagat tattaagtaa aggcaaagct ttttcgtgga cttttatatc
     3001 tacataaggg ttatcgactt ctccatgaat ttcaaaatca gtaaacattg taaatcgttc
     3061 tattgttaat ccaccattgc tttttcttcc gaaacgtaaa tccatcaaat gatcgtatgt
     3121 tcttgccaaa tcttttataa agacttctcc tgcagtctgc ttatacgcac ttaaatcttt
     3181 aagttggctg aaataaaagc gtattttttg attaccttta tcgcgcattc tagagacaat
     3241 agaaaaaaac aagttcattt ctattggggt aaattttctt aaaggaatgg tatttaattc
     3301 tggttcatac tttacgattt catttgccat ttagttgtta ctccagtcta aatgatttta
     3361 ttgacatcat tttaggacat attatgtcta aaaacaatac ctaaaacaga taatttcatg
     3421 cctataaaca gataatttca tgcctataaa cagataattt catgcctata aacagataat
     3481 ttcatgccta taaaattttg tatcccttgg gggagtaagg acacagccga cggtaaagag
     3541 gtttaaaaag aggtttaaaa aaagattata aaagagggag acaaaatttc ccacctaaaa
     3601 aaacaattat tttttcttta aacaggttcg cgaacttttc taaactgcta attcaaaacc
     3661 tcgctcccta cggtcgccaa aaaggtgtga tcgctacgct ctcacattat acctgcgcag
     3721 tcgcctccag ctcctttgct aaaaccattg ttcaaaaagg agggttgagg ctctaagagc
     3781 ttgtctaagc cgttttaaga cagtcttagt ataaaaacct ttatctggca tttaaagcgc
     3841 cctacaggcg gtcagggcaa gccctaacaa cccggaaata agagatactt aaaattgcca
     3901 cacagattta attttgtagg gtataataac ttgtcgccta tccgacagtc ttgcgactgg
     3961 aaggaggtgt atctgatgtt tgaactgttt atttgccccc ttcttgtagg agtttgtatc
     4021 tctgtattca gttattggtt gaataaccga aacaagaagt aaaatcagta aaggcgacat
     4081 caacctaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt
     4141 gaacatttgc atgtttagtt taacatgaat ttcaatcaaa ttcaattttg ctgttgctga
     4201 tctaaccatt ccttgacgct ttttaaataa tttaaagctg tttctttttt agtcagattt
     4261 tgtatgttaa ttgcttgttg aagcaagatt tcatagcgtt catatgtgac tgattgttgt
     4321 cgttcttttc gttcttgttc gctagttgaa aaaggaattg tatctccaaa taaatcacta
     4381 caagcttctt ccactttcgt atctttcaca tattgcacgt ctttttttac ttgttcatat
     4441 ttttcttggt actctttttg ctgttcttct ggcaaattcc gataaagtcg agtgagtgtt
     4501 tccatatttt tatccgtcaa acgcttttct tgcttcacaa aaatatcatt catgtcctca
     4561 taacaagcgg taaatttttc tattttttgt gctaatactt gctctttttt ctttttatca
     4621 cgcgctgatt catacttttg agtgacattt cctaataaaa tacttctaga aacgcgctta
     4681 tttccctcta aaacgccgaa taagccgctt gaaacgatta tcaaggaaaa tatacagcca
     4741 agaaagaaaa atcgcttaaa tcgaatttta gagcctttta atgaatatct tgtttgaaaa
     4801 attttataga tgagccaaat acttcctgaa atactaacaa aaatgagtag atagggtaaa
     4861 taaaatgcca ttttagcctc cccatactaa ccaaaatgcg aacagtgtca ggactaagca
     4921 acataagggg gcaagaaaat ccaatgatcg ttgaataagc acctttttct gttccttatt
     4981 tcgctggtct aatagctctt gatggagttt tttaacttgt aaatcaatcg tagtttgtat
     5041 cctttcttgc gtataaggaa cgagaaaagc acgttgtttc tttatttcgt tttctatgtc
     5101 ttttaacgtt ttagagcgat aattcttgtt ggtggtctgt tcctctttca acgtgttgtt
     5161 catggtttct tcgagccggt ctaatttccg gttgttcagg cgtaa
//
LOCUS       DACDYM010000119         5088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDYM010000119 DACDYM010000000
VERSION     DACDYM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.125"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(205..660)
                     /locus_tag="IWM59_002545"
     CDS             complement(205..660)
                     /locus_tag="IWM59_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980532.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1269..1685)
                     /locus_tag="IWM59_002546"
     CDS             complement(1269..1685)
                     /locus_tag="IWM59_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980533.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTITTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1687..1968)
                     /locus_tag="IWM59_002547"
     CDS             complement(1687..1968)
                     /locus_tag="IWM59_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980534.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2415..3383)
                     /locus_tag="IWM59_002548"
     CDS             complement(2415..3383)
                     /locus_tag="IWM59_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9980535.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(4724..>5088)
                     /locus_tag="IWM59_002549"
     CDS             complement(4724..>5088)
                     /locus_tag="IWM59_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9980536.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1595 a          839 c          839 g         1815 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt
      841 gtgatttcta attggtctag cttaaacact tcagccaatg cttgatttta tgcacttttc
      901 ttttttcatt tctttgtgca ttttgaccgg tcatttttta gagatttgtt atttttgtga
      961 gcatttgata tgctcttttt atacctcaat ctcttctttt ctaatgttaa tgaataggat
     1021 aatctcattt ttagctgctc gtgagagcag tctttttttg tttcagggta cgtcctattc
     1081 tttggatttc cgcgctatta aataaccgat acttggggat tggggtagcg ccccaaaata
     1141 caaaaagaag aagttgcata agcggatttt tctttttttt cgcaagttgg taccaacttg
     1201 caaatcttgc tggaagactg atccctaaaa agaaatcgag gaattttttt agggatcggt
     1261 ttcttttttt atctttttag aaagtttgtt gggtcaaaat ttccagaagt aaaggtttga
     1321 ttggtttctt tttgacgatt tttgggttga tttcgtcggg aaggattgag ttgattttca
     1381 attttttctt tcacttcttg atacacttta gaatttgtac cgataagaac accagtatct
     1441 tttagatata aatcagcaaa ttctgccatg atttctgtca tgatagaaat ttcttttccc
     1501 atggcattga gttttgcttg gttactggtt gtgattgtat tggtatcagg ttcataactc
     1561 atacataaaa aacgaatggc ttctgaataa ttgttgacgt ctggattttt ttcgatgatg
     1621 gaatctaaaa cgtcaacgtg actgggataa agggttacgt gtttttgaac agaagttttt
     1681 ctcatgttat attcctccga taagtgtttc aatgagttgg ttgtttgtgt gaacatagtc
     1741 attcagtatg ttcgtatttt ctgtttgaat ggcaataaat ttttcgagta ataaatcgaa
     1801 ttgatctttt ttgtaattca taagttgacg gtcaaaatca ctttcgatta gaacttttag
     1861 atatttattt cgactccatt ttttgcccgt tttggttgtg agttcctgac aacgtttatc
     1921 tattcttgca ataattttgg gattgatatt tcgaactaaa atgtccatgg aaattttcct
     1981 ttcgtatcag cgtataattt atgtatatca tacaaatata gtatttgtat agctattttt
     2041 cgccaaaaca ctatacatag cctagccaca atggcaaaaa agtgatagca aatttttttc
     2101 tgagaaacgt gataggcaca gcctatctag caccttttgt aagaaagtga taccacgttt
     2161 ccttatgctc tttcaaaatt cagaaaaatc agctgtaaaa cgagtgcagt ccgagtaaga
     2221 ttcaacgaaa aaacggggat tggggcgcca ccaaaatatc gcaacatggg tccatgttgc
     2281 tctgctcgca aaagcaaaaa gaagaataaa accagcaatt taaattaata aaaattgctg
     2341 gttttattgc cagaattgcc accatttttt ctccgtcttt gattgttttt tctgtcataa
     2401 aatctaaaaa cccctcaatc tttgaataag ttttgaagat tgtctaataa gtcttttttt
     2461 agttctgaag gaatatcgga atttatctct gatttttgtg cattattttc tttattttgg
     2521 gtattttgtt ctctttctaa cttttctaac tgttgtaaaa gttccaattt tcgcttttgt
     2581 tctttgattt tttcttgtaa tgaagtaggt gctttttcag attgaacttg ttggttagct
     2641 agttctaatg ctttgttttg ttcttctttt gttggggcat gtttgttaaa gtcgtctgat
     2701 ttttttatct caggtttaaa tgtaaattga tagccaatga caggctttcc ccgtcctttt
     2761 ccgtatttct ttttgattgt taaaccttta aagattgcag ttagttcttg tcgtattgga
     2821 ttgaacacac gttgatcaat cgtaccttgg ttgtaacttt taggaatatc aagtaattca
     2881 tgaaagtctt cttttgaaaa ataagcgaaa cctttggttc tgtattgttt tagtaatcga
     2941 aacattgttt ttgaataact gctttctaat tcattgaatt gttggagaga atagcgcacc
     3001 cattcttcaa gattattaag taagggtaag gctttttcat gaacttgtat ttcagcaaaa
     3061 ggaatatcag attttccgtc gattttaaat tgattaaata aaacaaaacg ttcacgttgc
     3121 aaaccgtcag cacttcttct accaaaacgt aaatccatta atttgtcata cgttgtttct
     3181 aaatcatcta tgaatcgaac atttgcagtt gctttataat tgcttaaatc ttttaattgc
     3241 tcgaatgtaa attgtacttt tttatcacct acatcacgca ttcttgatac gatagaaaag
     3301 aacaaattca tttcgactgg tgtaaatttt cgcaacggaa ttgtatttaa ttcatgatga
     3361 tactttacga tttcattaga catatttaaa atagctcctt tcttactcgc acaagagata
     3421 cacgttatga aatacaaaat gcctatttat ctacatctta tattaacatc gtcgatgtac
     3481 taattcaatg tacaaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa
     3541 cagacaaaac gatgtagata aacagacaaa acgatgtaga taaaagcgtc attcccttgg
     3601 gagagtaagg atatagccga cggtaaagag gtttaaaaag aggtttaaaa agagattata
     3661 aaagaggggg acaaaatttc ccgcctaaaa aaccacttct tttttctcca aacagattcg
     3721 cgcatttttc tcaactggga atcttactcc ctacagtcgc ttaaaaaggt gtgatcgctc
     3781 tgctctcaca ttatactgcg cagtcgcttc cggctccttt gctaaaaata ttcataagga
     3841 actctttgag aagctatgag ccttcgtttg agacgtttta agacagtctt agtataaatc
     3901 ccttatcttg ttcttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaag
     3961 ttgtctgaat gccctctaaa attcgattta agcactctta aacctattct tatatattta
     4021 tatacttaga cgtttcaaac gtcttaaaac gcaaattaga gcttgataaa gatatttgct
     4081 cgtgtgccga cttttttaaa agtctaggca cacgccatga caaagctgaa ataaactgct
     4141 tcagctttgt catggcggat tcgctcactt gaaaaagtgt tcatttcgct cgattattag
     4201 catatatttt ttaacttttt ttgctagcca aattttgcat tagttcgctc ataaatccca
     4261 ccctttcagt acgtcctacg gacgtatcgt tcgtaatgtg aaagtacctt taaaggtact
     4321 ttctacaaaa ctcactaaag ggtttggcaa gctctatatt tgctagccaa atacctacgt
     4381 atttggtgca aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat cccccctcaa
     4441 aacccacgct ccacaataca aaaaaggcta gcaaatgcta gccacttttt gtaaagtttc
     4501 gcttgtttca ccttcggtga aattgttgct ttgcaacgat caggtagagc tacatttttt
     4561 ttcgttgttc aaatattctt acgccattta ctattaaaat cgtaggtatt ccaaagtaaa
     4621 gaaaaaattg attagcaggt aatacaaaat ataaaataat accagcaata attaccaaac
     4681 ttatagtaag ttttgttaat ttcatgaagt atgccacctt atcttatata gatatcaaat
     4741 agattaatat gccgattgta ttataaaatc catgagcaac tatggaatac catatatttt
     4801 ttgtttgtat ataaacaaaa cttaaaataa taccaataaa gaaaatactt aggcctcccc
     4861 catgaacaat agaaaacaga aaagaagaaa ttagtattga aatatattgt tgttttttgt
     4921 ttgcttgagg atcaatcata caaaatcgaa aaattccttc ttctaaaaaa gctccataag
     4981 tagcagcatt taaaaagaat aaaaggagag aaccatttac ttcattagga tttatacctt
     5041 ctgaaaaaga tgtatttaga atattgatta ggaataaaac agagaatg
//
LOCUS       DACDYM010000120         4805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDYM010000120 DACDYM010000000
VERSION     DACDYM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.126"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..411
                     /locus_tag="IWM59_002550"
     CDS             <1..411
                     /locus_tag="IWM59_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980537.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="IWM59_002551"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="IWM59_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP9980538.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /locus_tag="IWM59_002552"
     CDS             2446..3708
                     /locus_tag="IWM59_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP9980539.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4805
                     /locus_tag="IWM59_002553"
     CDS             3953..>4805
                     /locus_tag="IWM59_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP9980540.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1660 a          665 c          979 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaagaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata atgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaaa
//
LOCUS       DACDYM010000121         4771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDYM010000121 DACDYM010000000
VERSION     DACDYM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.127"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            192..290
                     /locus_tag="IWM59_002554"
                     /pseudo
     CDS             192..290
                     /locus_tag="IWM59_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(513..743)
                     /locus_tag="IWM59_002555"
     CDS             complement(513..743)
                     /locus_tag="IWM59_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9980541.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(973..1791)
                     /locus_tag="IWM59_002556"
     CDS             complement(973..1791)
                     /locus_tag="IWM59_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9980542.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1952..2641
                     /locus_tag="IWM59_002557"
     CDS             1952..2641
                     /locus_tag="IWM59_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9980543.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2655..4157
                     /locus_tag="IWM59_002558"
     CDS             2655..4157
                     /locus_tag="IWM59_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9980544.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4170..4646
                     /locus_tag="IWM59_002559"
     CDS             4170..4646
                     /locus_tag="IWM59_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9980545.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1595 a          696 c          847 g         1633 t
ORIGIN      
        1 accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt tactgataga
       61 cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat tttggcaatt
      121 tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat tcaaacatta
      181 gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga acacctttgg
      241 aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga caattaagag
      301 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
      361 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      421 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      481 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      541 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      601 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      661 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      721 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      781 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      841 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      901 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      961 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
     1021 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
     1081 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
     1141 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
     1201 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
     1261 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1321 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1381 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1441 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1501 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1561 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1621 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1681 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1741 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1801 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1861 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1921 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1981 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     2041 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     2101 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     2161 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     2221 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     2281 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2341 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2401 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2461 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2521 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2581 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2641 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2701 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2761 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2821 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2881 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2941 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     3001 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     3061 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     3121 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     3181 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     3241 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3301 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3361 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3421 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3481 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3541 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3601 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3661 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3721 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3781 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3841 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3901 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3961 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     4021 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     4081 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     4141 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     4201 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     4261 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4321 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4381 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4441 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4501 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4561 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4621 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4681 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4741 ttttatttta ttagtaatga ttccccatgc c
//
LOCUS       DACDYM010000122         4735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDYM010000122 DACDYM010000000
VERSION     DACDYM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.128"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IWM59_002560"
     CDS             25..696
                     /locus_tag="IWM59_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9980546.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IWM59_002561"
     CDS             755..1195
                     /locus_tag="IWM59_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980547.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IWM59_002562"
     CDS             1207..1875
                     /locus_tag="IWM59_002562"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9980548.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IWM59_002563"
     CDS             1939..2562
                     /locus_tag="IWM59_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9980549.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IWM59_002564"
     CDS             2618..3004
                     /locus_tag="IWM59_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9980550.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IWM59_002565"
     CDS             3217..4608
                     /locus_tag="IWM59_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9980551.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1577 a          782 c         1007 g         1369 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaat
//
LOCUS       DACDYM010000123         4710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDYM010000123 DACDYM010000000
VERSION     DACDYM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.129"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            105..356
                     /locus_tag="IWM59_002566"
                     /pseudo
     CDS             105..356
                     /locus_tag="IWM59_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            449..1243
                     /locus_tag="IWM59_002567"
                     /note="aph(3')-IIIa"
     CDS             449..1243
                     /locus_tag="IWM59_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9980552.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1653..1764
                     /locus_tag="IWM59_002568"
                     /pseudo
     CDS             1653..1764
                     /locus_tag="IWM59_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1785..1868
                     /locus_tag="IWM59_002569"
     CDS             1785..1868
                     /locus_tag="IWM59_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9980553.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1993..2730
                     /gene="erm(B)"
                     /locus_tag="IWM59_002570"
     CDS             1993..2730
                     /gene="erm(B)"
                     /locus_tag="IWM59_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9980554.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2993..3856)
                     /locus_tag="IWM59_002571"
     CDS             complement(2993..3856)
                     /locus_tag="IWM59_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP9980555.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3858..4130)
                     /locus_tag="IWM59_002572"
     CDS             complement(3858..4130)
                     /locus_tag="IWM59_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9980556.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4147..4362)
                     /locus_tag="IWM59_002573"
     CDS             complement(4147..4362)
                     /locus_tag="IWM59_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP9980557.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4454..>4710)
                     /locus_tag="IWM59_002574"
     CDS             complement(4454..>4710)
                     /locus_tag="IWM59_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP9980558.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1603 t
ORIGIN      
        1 tcttgggagt tttgagacta ctatatgcgt ttttttgttg atttaccaac atgtttagac
       61 attttcctct aggaaaaaat taactagcac cacgggttat cttttatata gaagatatcg
      121 ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag
      181 aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta
      241 tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
      301 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
      361 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
      421 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
      481 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
      541 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
      601 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
      661 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
      721 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
      781 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
      841 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
      901 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
      961 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
     1021 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
     1081 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     1141 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     1201 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt
     1261 ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa
     1321 ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagatagg ggtcccgagc
     1381 gcctacgagg aatttgtatc gataagaaat agatttaaaa atttcgctgt tattttgtac
     1441 atttaacttg acggtgacat ctctctattg tgagttatta gtggtacagt tttcaaccgt
     1501 tttaattata aaaaagtggt gcatttttaa attggcacaa acaggtaacg gttattgcag
     1561 gtgtatttct tatctatggg tttaacatgg attttatcat taaaatcatg agtattgtcc
     1621 gagagtgatt ggtcttgcgt atggttaacc ctaaagttat ggaaataaga cttagaagca
     1681 aacttaagag tgtgttgata gtgcattatc ttaaaatttt gtataatagg aattgaagtt
     1741 aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg gtattccaaa
     1801 tgcgttatgt agataaaaca tctactgttt tgaaacagac taaaaacagt gattacgcag
     1861 ataaataaat acgttagatt aattcctacc agtgactaat cttatgactt tttaaacaga
     1921 taactaaaat tacaaacaaa tcgtttaact tctgtatttg tttatagatg taatcacttc
     1981 aggagtgata acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa
     2041 gtactcaacc aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt
     2101 ggaacaggta aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct
     2161 attgaattag acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt
     2221 gtcactttaa ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa
     2281 attgttggga atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa
     2341 agccgtgcgt ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat
     2401 attcaccgaa cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag
     2461 ctgccagcgg aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc
     2521 cgccatacca cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa
     2581 tgggtcaatc gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa
     2641 cacgccaaag taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat
     2701 agttatctat tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa
     2761 gttacacgtt actaaaggga atgtagataa attattaggt atagggcacc tctaataact
     2821 accaattaat tcacctcaaa atgaaattag aaaaaaacag cgaaattttt aaatctattt
     2881 cttatcgata caaattcctc gtaggcgctc gggaccccaa catgaggaaa tgtttttata
     2941 aaaaaagagt agttaccgtt ttttggtaac tactcttttc cttttttaag gtttaaatac
     3001 ctggaagttt aggtgttttg ggtattggtg gtgtaggtgg ctgcaagctt tccaattttt
     3061 gttgaattgc tttaaattca ggtgtctctt ggtgtttatt tagaaccatt ttttgctcta
     3121 ttcgttctaa agtcggttga atttctttcc ctgatacttt acgattcaat tctttttcta
     3181 aggtttcttt tggactaata gaaggtgttt ccaaacttga atagagtttt actccttctc
     3241 tgttatatag ccttatatcg ctaaataagc ccgttttatg aagggtttct aaattggtcg
     3301 gtaagttttt gacaacaata tcatgcgctt gttttggtgt tgccctggct gtcattggat
     3361 catctgcata catggtttca tatcgttcaa ttgttcctaa atatgagttg attttaggta
     3421 ctgccatgac atacattttt gtttcataac ctttggcttg aagcattgtt gcggtttgaa
     3481 taggaacgtc tgttgttcgt cctgtacctt cgatcaccaa attataccct tgatcgctca
     3541 aacggcttat gatcgcttct gtcatgcgat tagaataagg ggtaacgtgt tttactacgt
     3601 ctttttcata aagtttcact agttcatcaa aattagggtg ctgttgttta aaggtatcat
     3661 tatcaatgac aataacattc ccttgtgttt cttcaaaaat tgctgatcgc aaactggttt
     3721 tccctgaccc tggttgccca ccaagtaaaa aagcggttgg cgattcaacc gctttttttc
     3781 cttgaatcaa ttcttctaaa ttatcattta agcgattctc aaattgtttg tcagtaaaat
     3841 tgactatatt tgccatatta agccactttc tctttattca aaacttgttt tgagtaacga
     3901 ttcattgacc gccaatactc atgaacggct tgtaattctt ctaaagaata atcaaaaaga
     3961 tttacacgtt ttgcaagctt taattggttt aataaattga cttccaataa ttgagagtca
     4021 tttttattta attcatggtt caaaacatag tttaatactc gattataaac gcttgccgat
     4081 aattcgttaa tgatctctat ttcaaatgtt ttttcatacg taactgccat ttcattttca
     4141 ctccttttaa agtttgtcag gtaaatattt ccgtatatat tcttctaagg cttgatccat
     4201 aacttctttt acgtttccgc cattctttgc tgtttcgatt tttatgatat ggtgcaagtc
     4261 agcacgaaca cgaaccgtct tatctcccat tatatctttt tttgcactga ttggtgtatc
     4321 atttcgtttt gctttttgtg cgcctaaatt tcccacaatc actcacttct ttctatttct
     4381 tcttactctt attttatcat caacaatcac aaatcacaag tgatttgtga ttaatcactt
     4441 gtgatttgtg attctattct ttttcgtttt ctaattgaat aattcgctca agcatttcaa
     4501 aaaatacgtt cttatacatg gataaaactt ttttgtcata gcctttatgc tctgtaatgc
     4561 cgtttttaga ccaggtactt actttattac ttcgcttgat aatattttgg aaaaccaagt
     4621 tatcttcttt atgttgctta tacagttctt ccaggtttga ttttatcgtt gcgctgtccg
     4681 tatcaactaa ataaggaaca aaaccgatca
//
LOCUS       DACDYM010000124         4694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDYM010000124 DACDYM010000000
VERSION     DACDYM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.130"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            160..390
                     /locus_tag="IWM59_002575"
     CDS             160..390
                     /locus_tag="IWM59_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9980559.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            487..1788
                     /gene="murA"
                     /locus_tag="IWM59_002576"
     CDS             487..1788
                     /gene="murA"
                     /locus_tag="IWM59_002576"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9980560.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1801..1977
                     /locus_tag="IWM59_002577"
     CDS             1801..1977
                     /locus_tag="IWM59_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9980561.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2112..2408
                     /gene="yidD"
                     /locus_tag="IWM59_002578"
     CDS             2112..2408
                     /gene="yidD"
                     /locus_tag="IWM59_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9980562.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2592..3059
                     /locus_tag="IWM59_002579"
     CDS             2592..3059
                     /locus_tag="IWM59_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9980563.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3144..4028
                     /locus_tag="IWM59_002580"
     CDS             3144..4028
                     /locus_tag="IWM59_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9980564.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4145..4378
                     /locus_tag="IWM59_002581"
     CDS             4145..4378
                     /locus_tag="IWM59_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9980565.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4466..4546
                     /locus_tag="IWM59_002582"
     tRNA            4466..4546
                     /locus_tag="IWM59_002582"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4500..4502,aa:Tyr,seq:gta)
     gene            4553..4624
                     /locus_tag="IWM59_002583"
     tRNA            4553..4624
                     /locus_tag="IWM59_002583"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4585..4587,aa:Gln,seq:ttg)
BASE COUNT         1574 a          735 c         1026 g         1359 t
ORIGIN      
        1 ttgtctcagt cccttttttt gcatattttt agcatttatt tcctaaaaaa tgtaagggga
       61 ttcctatatt tttaagtata cttgtttttc cttttataca ttttatggta ttatcatttt
      121 attagttatt ataaaacaat cgttagaaag gacgtcatta tgcaagtata cggaattgat
      181 gcggtgcttc gaatcgtgag tcatttggcg tttatctact tagccttctg gtctttacgt
      241 tcgttacgca ttgaaacttt tttcagacca atgcataata cgcaaattag aatggcaatc
      301 gtactttttt caattttttt agggtacact gcaagtaatt ttttcttaga agttatcgct
      361 ttatgccgaa atcttttcgt tagcctgtaa attttaaaaa aaggtactat tttaaaagca
      421 aggatgttat aatagaaaag attttgagta agtgtatgct cattggaatt ttttggaggg
      481 aacaacatgg aagagatcat cgtaagaggt ggcaatcaat taaatggaac agtaagaatt
      541 gaaggagcta aaaatgctgt tttgcctatt ttagctgcta gtttgttagc agaagaaggt
      601 attacgacat tagacaacgt tccgattctt tctgatgtct tcactatgaa tcaagtgatt
      661 cgtcacttaa acgtagatgt agattttgat gaacaaaaga atcaagtaac aatcgatgct
      721 tcaagacaat tagaaatcga agcgccttat gagtatgtta gtcaaatgcg tgcttcaatc
      781 gttgtgatgg gtccgctact tgccagaaat ggacatgcaa aagtagctat gcctggtgga
      841 tgtgcgattg gtaaacgacc aatcgatttg catttaaaag gatttcaagc tttaggagca
      901 aaaattattc aaaaaaacgg ctatatcgaa gcaattgcgg atgaattgat cggtaacacg
      961 atttatttag atttccctag tgtaggtgct actcaaaata tcatgatggc tgccgtaaaa
     1021 gcaaaaggaa caaccatcat tgaaaacgta gctcgcgaac cagaaatcgt tgatttagca
     1081 aacattttga ataaaatggg cgctcaagtc tatggagctg gtactgaaac aatgcgtatt
     1141 gaaggcgtgg atcatttaca tgcagtcaac cactcaatcg tacaagatcg tatcgaagca
     1201 ggtaccttta tggttgctgc tgcaatgaca caaggcaatg tgctgattgc agatgctatc
     1261 tctgaacata accgcccatt gatttctaaa ttgatcgaaa tgggtgcaga aatcattgaa
     1321 gaagaaggcg gagtccgtgt catcggacca aaacatatct tacctacaga tgtaaaaacg
     1381 atgcctcatc ctggtttccc tacagatatg caagctcaaa tgacagctat ccaattagtt
     1441 gcagaaggta caagtgtagt gactgaaaca gtgtttgaaa atcgtttcca acatttagaa
     1501 gagatgcgcc gtatgaacgc tcatgtcaaa atcgacggaa atgtagcaat catggatgga
     1561 aaccatgaat tgcaaggtgc agaagtatat gcaacagact tgcgggcagc agcagcatta
     1621 gttttagctg gcttgaaagc aaatggaatc acacgtgtgc gtaacttgaa ttatctagac
     1681 cgtggatatt ataatttcca tatcaaacta cagcaattag gtgcagacgt tgaacgtgta
     1741 gacatggatc aaacttctgc tgaaaaaaca gcacaaacga ttgcttaagg aagtggctga
     1801 atgagtaacc aacgatatat catattaact ttgataaaga ttttagtggt gatcttattg
     1861 cttatccttc tttttgtggc cggaacgatg atcggatatg gtgtcatcgg tggcggcaat
     1921 ccatttaaag tattccagcc aagtttgtgg attcatattc gagacttttt tcattgataa
     1981 ataagaatca cagtggttct agtcgatatg aagtgagcca tgaagcagaa catgacaaaa
     2041 gaaaacagaa gggaacgttg acgttagtcg cgatcccttt ttttagtaaa agaaaagaag
     2101 gaatctaatt gatgaagaag atattgattt atttagttag aggatatcag cgctttattt
     2161 cgcctgcatt tccacctagc tgccgttatt acccaacctg ttccaattat atgataaaag
     2221 caattcaaac ccatggtgca ctaaaaggga cggtaatggg gactgcacgg atcttacgct
     2281 gtcatccttt tgtcaaaggc ggtatcgatt atgtaccatt gacattccaa ttaacgcgaa
     2341 ataagaaaga taaaaaagaa ccagattata aaaaggttgt gaaaaatacg agtagcactg
     2401 agcgataaag aaacaatttg gaaaatagct tcttatattt tgcaaaattg tgagttattg
     2461 ccgaagtgtt ggattttgaa caccgtcaaa aaaaggttgt gaaaaatacg ggtagcactg
     2521 agcgataaag aaaaagagga aaattattgt caaagtaaca actccgcaac tacttatatg
     2581 ggggacaaaa aatgatcgaa gaactaaaca agaaagattt accatggtcc ttgttattag
     2641 atgcagatcc tgataaagaa aaagttcaag tatatgtttc cagagggagt ggctggatat
     2701 ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa aaagaatacg
     2761 aaatcgtcaa tgtagcagta gccccaacgt atcaaggaaa ggggattggc ggaaaattac
     2821 ttgagacagc ttttcagaag ctttcacaac tcgcttccag tcaaactcag atcatcatac
     2881 gaaccggcag tactagcagt gcggcgttac atctctatca aaaaatgggc tatgtggaga
     2941 ttggtcgagt caaagactat tttatccatc attactctga gcccattttt gaaaatggag
     3001 atcaattaag ggatcaagta attttagcaa ttcacttacc taaaaatagc tttaactaat
     3061 aaactttcaa ttcgagaaaa tgctttcaaa tgctaaatag atacattaca atagctttag
     3121 caaacaaaaa ggagaatatt tttatgggaa tattagtttt tgattttggt ggatcggctg
     3181 tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg
     3241 aatcttggga agaaatgaaa gcgcaattat ttcaagttta taaaaaaata ctcgtttctt
     3301 ttgaagtcga aggaattgga atcagctctc ctggagtagt gaataatgag aaacaggtga
     3361 ttgagggaat cagtgcgatt ccttatattc atggatttaa tattttccga gatttggaaa
     3421 ctctgtttca actcccagta acgattgaaa atgacgccaa ctgtgccgga atggctgaat
     3481 tttatgaagg tgctgcccgt gattatcaag atgttgcttt tgttgtggca ggaactggga
     3541 ttggcggggc gttgttcacg aaaggacaaa tcaataaagg ggcacatctt tatggtggag
     3601 aatttgggtt gatgttctta aatggcgata agacattcag taaacttggt acagcggtac
     3661 agatggcttg gcgttattgt gaacgcaaag gtctcgataa aaattcttat accggaaaag
     3721 atgtctttga attagcagaa actggagatg caatagcaaa agaagaagtt gagagttttt
     3781 atacgtatct tacaaaggga ttattcagta ttcaattttc gtttgatcca gaagtgatcg
     3841 tattaggcgg cggtgtttct gcaaaagaag ggctcattga tgagatcaaa agccgaatga
     3901 agaagctgac agaacaattc gatctgcatg atttcgaacc gcagatttta ttatgtgaat
     3961 acagaaacga tgctaatctt gtaggtgcag cagcaaacta tctcgctgta cgtcaagggg
     4021 agaattaaga gctttagaga aggtgcagct tgcataaaag aagtgaatga aatgaatagg
     4081 agatttttac ctaaggaatg ttttatttgc tgcagtatga taaaatatct gagaggtgaa
     4141 aatgatgtca aaaaaaaata aagatgttga agtgcgtata gaagaagtga aaaaagatat
     4201 caaaggtaag aattatgaag tcactcagtt atttataggg aaaaaaatga ttggtgagat
     4261 cttagcctat gggccaaaag aatatgaaat tttctttggt gaagaagatt ttggaaaaga
     4321 aaaaagtctt gaaaatgcga tagaaacagt gattcgacac tggaatctgc acgaataaaa
     4381 aaatatcaaa aaaactttca ataaagggtt gcaaaaatct ggaaaattag gtattataac
     4441 taagtcaggt tgatacaaac ctttcggagg agtagcgaag tggctaaacg cgacggactg
     4501 taaatccgtt ccttagggtt cagtggttcg aatccactct cctccatttc attggggtat
     4561 agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc
     4621 ccagtatacc acaatcgtaa gctctcgtta gttcgagagc tctttttttt gctttgctac
     4681 cttgttcctt gaag
//
LOCUS       DACDYM010000125         4608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDYM010000125 DACDYM010000000
VERSION     DACDYM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.131"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(83..337)
                     /locus_tag="IWM59_002584"
     CDS             complement(83..337)
                     /locus_tag="IWM59_002584"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9980566.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(448..702)
                     /locus_tag="IWM59_002585"
     CDS             complement(448..702)
                     /locus_tag="IWM59_002585"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9980567.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(895..1170)
                     /locus_tag="IWM59_002586"
     CDS             complement(895..1170)
                     /locus_tag="IWM59_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9980568.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1142..2095)
                     /locus_tag="IWM59_002587"
     CDS             complement(1142..2095)
                     /locus_tag="IWM59_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9980569.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2707..4200
                     /locus_tag="IWM59_002588"
     CDS             2707..4200
                     /locus_tag="IWM59_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9980570.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4335..>4608
                     /locus_tag="IWM59_002589"
     CDS             4335..>4608
                     /locus_tag="IWM59_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9980571.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          801 g         1541 t
ORIGIN      
        1 cttgaattct tgtgaaaaag ttcttcgtgt tctcttagac ataaaaattc ctcctggtat
       61 gttttcttct agtctacaca ccttaatttt tctgtctagt taattgtagc ctatccatga
      121 tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt
      181 gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt
      241 gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct
      301 ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat
      361 agacacttct agggatttct aataaccgac acaacaaaga aacagtgtaa tccttacacc
      421 atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt
      481 ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc
      541 ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt
      601 tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag
      661 ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt
      721 agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc
      781 aaatgttgta tacccccaaa caatacacat attttgaagg attaggatta ataaagctaa
      841 aaatatagta agtatactaa atatatttac tatatctagt atacttacta tattttaatc
      901 ttttgatcgt ctattatata taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc
      961 cattgcaagt ttgactattt catacatagg aatatctttt gcataactaa gatcccgaat
     1021 ggtttttagt acgggaggat ctacttgaac tggttttctt tctttcgcag tgtactttcg
     1081 ttcccctaac tcgacattaa tatcatcttt aattgaaata tcttcagcac caattttctt
     1141 cttatctttg ttcgttggta ataatgccat ttagtttaat ctcctttcca agaggtagga
     1201 tatgattggc cattgagtca gcatatttag gtgtataatc aaatccttca atatcaccat
     1261 gttgttcata gtattcaatt ctttcttcta attctgtaaa gatgtctgca aaaatagccc
     1321 accatcttcg atctacataa tcatttaatt ggataccatt ctcaccatac atttctaacc
     1381 gatccgagcc tttaacaatg cttttaaaaa tgtttgattc accatactta tcaattgttt
     1441 tttgatagtt tgcgtgttga gaggaacgtc gcactgagaa aagaacaggc aaaattccaa
     1501 tgatttgaag atgagactct tcaaaattcg tgacaatcgg aactaaaact ttattaataa
     1561 aatcttctgt tccttccatt gcgtaccgtt ttaattcttg cattgcaata atataatctg
     1621 cagcagttaa gaatgcacga accacattat ctgttgatgg tgggcagtcg ataaagatat
     1681 aatcgaaatt ttttctcaat tcttctaaag gtttaatgaa ggccaatgat ctcttacgac
     1741 gatcaggata tttttcggac aagtattcat taagttcttc agatccagga gagccagcca
     1801 taatatataa atgtggactt acttgagtaa tacctgaaac ccagtcccct tttttgattg
     1861 ctgcagacat ggatcgatca aagctagtaa tttgatacgt tgatccaatg ttggaagtag
     1921 tatttctttg ttggtcataa tcaattagca acacgtcttt ttcaaaaagt tgactagaaa
     1981 caatcgctaa taaatcattg tctgtagtct taccaatgcc gccttttcta cctgtaagca
     2041 ttacaacttt tcctagttta tctgatgttt taaatgcagt ccgtaagttt tccatagttc
     2101 cctccaattt cctatactaa atatatttag tatacttcat atacttaata tattcgatat
     2161 atctaatata ctccaaattg ataagaattc agactttata aggaatatag tggatatact
     2221 aaatatattt gctatattca gtatatcaga tatattcaaa atgtcaaaat atatttgtgc
     2281 gttttgcgtt gacaatttag agtgaaaaga ggtaaactta attctataga aaccacagat
     2341 aattcactat ttacaaagta gcaaaagcag tggtactctt taaacagaaa aattacataa
     2401 aaaaaagaga cggaaataat tgattttagc agaatcaatt atttctgaat cacatggtta
     2461 ggacatgtgt tcacgctcgt ctcaagaata tgtaagtagc taccaactac ttatattaaa
     2521 agttgatcgg ataccttttc cgattgcctt tattctacca aaaaaaggcg agcaactcaa
     2581 cagaagaacg tttatttagt gtacttaaat taaatattct taaacactga agagagaaca
     2641 catactatgg ctgagattaa agctaaactg tgttctcttt ttgtttgaga aaggacgtta
     2701 caatcgatgt tgagcgaaat ttattcagct attcttaaaa acggcttacg tacatacaaa
     2761 tatcgaaaca gcaccatgaa acctgcaggt catattgatt ccagtaggag tggtgctatc
     2821 tttgcgtacc gtagcaagca attaatgaat gttgggcgtg gtatggtcat tacttcggaa
     2881 gaagctattt tagagaacga aaagaaacta acgcattgga cacctaatac ctatcgcttt
     2941 ggtacttatg cggatgatta tcgaacagtg gttaaaggtc attctgagaa aaacttaagt
     3001 caaatcaaca catttgtagt tgatatcgat tcaaaagaga accatcaagg cgaaattatt
     3061 ttagcctgtt tagatcaagt gggctacatg ccaacgctaa ttttagaatc agatcatggt
     3121 tatcaagttt attttgtact aaagacacca ggctatgtca caaagaaaag caatttcaag
     3181 gtgatcgatg tagcaaaaaa aatttcaaaa acaattcgtc tacagttggc tgaaaaattg
     3241 tccggagtgg atctaggatg taaccacttc ggaattgctc gattcccaca agaaaataat
     3301 attgttttta aagaaatgaa ctatcagtat tcttttgctg actggattca atggtcgatg
     3361 aagcttgaat cagatcaaaa aacaaaagaa gatcgagaac gaaaactaat tgtctttcct
     3421 gagaaaaaag aaattcgtca agtagatgaa ccgtggttcg atatgttact tcataaagca
     3481 aacatcgtcg gcggagaagg acgtcttggt cgaaataatg tgattttcac gctttcttta
     3541 gcttattact cttcaggtta tgcattggat acttgcgaat acaatatgtt cacattcaac
     3601 gaacggttac aggaaccgct atctgaagca gaattagtaa agattgtacg tagtgcttat
     3661 tctggtaatt atcaagcagc aagcagagaa ttcattattg aattgtgcca gcaatgggtt
     3721 tcaccaacct taaaaaataa tgatctgttc attcaacgac gtggctggtg gaaatttaaa
     3781 aagccacgag atcaaagaaa atatagtcat aaaaatgaat ggaaagaaga tctattggct
     3841 tatctaagcg aaaaaagtct tgctagtgag ccttatttga acctgtcaaa aaaagaattg
     3901 gcttcacaac taacgatgcc caagcgaaca cttgataaga tcttaaatga gttacgagag
     3961 gagaataaag tcttctacaa ggtaacacga ggaagaaatg gcggaatgat cattgcttca
     4021 gtcaaaacgt tgttcgcgag ggttattcaa ctaagtaaag aaaagagact agattattca
     4081 acaaggatca cagaggtatt tggactcaac gagagcttag tagaagagac atttagacaa
     4141 ttatcaaccc ccttgaaccc tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga
     4201 aaaataaaag gtgcaaattg ccttatacaa tatatctatg atacttctgt aggtgttgct
     4261 ccctttgcct agctacgtgt aggtggtata cttataactg cgtatgcagt cttatgtatc
     4321 atggaggaag ttgtatgtcg ttaaaaaatt atgtctattc acatcctgtt aatgtgttta
     4381 tcattggtcg tttgggatta tctgtggaag tgttctgtga gctttatggt tttaagcagg
     4441 ggactttgtc ttcttgggtt accagggaaa aaacagttgc ttctttgccg attgagtttc
     4501 ttcatgggtt gtccttagct tctggggaga ctatggatca ggtatatgat tgtttgtgtg
     4561 tgttagagca ggagtatatt gagtataaaa tagctaacga gttaagaa
//
LOCUS       DACDYM010000126         4581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDYM010000126 DACDYM010000000
VERSION     DACDYM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.132"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1605..2759
                     /locus_tag="IWM59_002590"
     CDS             1605..2759
                     /locus_tag="IWM59_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP9980572.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2753..3175
                     /locus_tag="IWM59_002591"
     CDS             2753..3175
                     /locus_tag="IWM59_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980573.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3477..3857
                     /locus_tag="IWM59_002592"
     CDS             3477..3857
                     /locus_tag="IWM59_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9980574.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3839..>4581
                     /locus_tag="IWM59_002593"
     CDS             3839..>4581
                     /locus_tag="IWM59_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9980575.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQ"
BASE COUNT         1719 a          781 c          765 g         1316 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaatgttag taaatattag aggtaaacaa actatgcgta cttgttacct tatgagttgg
      181 aatgctagta gtagaacagc caaatattat tactacattt agtaaaaaca aaacattctt
      241 tctatacaat aaaatagcgt atagagagga tgtttttaat ttgaaaagga gtggttatct
      301 ttatgaaaaa tagatcattt gctatattag tattatccgt aattttatta gatatattgt
      361 taataacatt agctttaaaa actttaaatt tagtaacaag ttttgtcatt ataccagtaa
      421 ttataggaat gtctatttat ttcatcaaaa aaaataaaca atttttttga atgaaattac
      481 gggcgaaaat aatcactcaa aattattaat aaaaacgaat caactacttt ttctgaagaa
      541 acatacaacc gtaaacaaca attgttgttt acggttgtaa attatcaaac tatatggtga
      601 cttgttgctt aaaataggtt gaaatttcgt ttattttttt tgagaaattt tacccacgta
      661 ctacccatgt acctatccac gtattaccca tgtacccccc catgacaaat gggcggaggg
      721 gcttgcggcg cttggtatta aaggcattat ccgcctccca ggtttctgac cattttctct
      781 cataagtggg gtttttaccg cgttgagaga aaatggtcag aaacctaaac gatcggcaaa
      841 gaaaacatac caagcgccac aagcaaaaaa agttagagta acttccagaa aagaaaaatt
      901 tttagaacaa cttttgaaaa tttgattttg atttatatcc gttcaattta aacatttgat
      961 aaactgaagg ctagtattta aaatgtgtat acaagtggta gcgttaaaaa aagtggaggt
     1021 ataacctatg ttaaaaaaaa ctgtgttggt actgggttct atttctctag gaatattagg
     1081 ttttagcccc atgctattat ccgtaattgg aaaaaacaat tccaacagag aacttaaatt
     1141 aaattcaaat tattacaaca gtcctaacga agaattgttt gtaaaacaaa cggataaata
     1201 ataaaaaaga ctgctctttc gagcagccag ttaaaaaata atgagtgttt attcttgaaa
     1261 aagcacgcgc ttatgaagcg ctaccaaaaa tttaccatat tttacaaaaa ataacaagtc
     1321 ttaaaaaaat gacagataaa aatgcacaaa gaattagata tagataaccg cgaaaaatca
     1381 agctttagaa agttgctttt agctagacca attaaaaatc acaaagtgag cgtctctatc
     1441 cattggtaga ggggctactt ggcatagtgt acattatgtt aactaacgct ataaataacg
     1501 attatgttcc caaaacaata ttttatgaac tataacaaaa atttgttatc gcatattgta
     1561 tattttaatt ataggtgtat accacttaaa tttaacacaa aggcgtgatt attttgaaac
     1621 ccaaaaagaa aatttatcta ggtctcctct tttccttgat gattggaatt tttagtatag
     1681 gaattacaaa tacctatgct tcagaaaatt ctaatttcaa tatcaactcc caaccaattg
     1741 aagaacaagc aattaatttt gcgaaaactg attttgaaca ctccataact tcaattcttt
     1801 taatggaaaa taacaccgaa actgatatta actatactct tggttcgcca tttaaaatgc
     1861 tcgatggaga aaatacggca aatagttcta ttttctttcc agtattttca gacgctgata
     1921 atacgattaa atatatttat accgtcaaaa aaaatacaaa tggagaatat tcaggaagta
     1981 tttcacaatt tttagcaaaa gaacttcaaa atctagtaga tagtaatata acagatacaa
     2041 ccttattttt tcaagataat agcatttatt attccgataa tggacaaatt aaactacttt
     2101 ggaaatcacc tttaagtaca gaaaaaaatg gtgtttcttc ctttacagaa aaaaatgatc
     2161 ttatatccgt aaatataaaa aataaaatta aaaatttctc taaaaaacaa aattttcctg
     2221 cgattaaacg tgtaaaaagt gtctcctccg gagataactt tattatgatt aattggcgta
     2281 tagcagaaac ccaaacaagt tttccgtggt gtgctgctta cgtagaagct gctatcttat
     2341 gtaacaaaac agatgtacat gctacttcag ctagagccat tatgcattat acctatccta
     2401 atttaaacaa cagtcaaatt gaatcaaaaa gtcttaatca agatcaaata ataaaatatg
     2461 caaattactg tggtgtttat cctaaaaaag tagcacgaat actaaacctt tcagaggtcc
     2521 aaacacaact aagaaaagga aatgccattt atatgggaac tgctggttat ggaaattata
     2581 gtaacagccg tcatgcactt gcattatttg ggtgggtcaa tcaaggtggt tatcaaactt
     2641 actatgtgtg gaatccttgg tataactacc caactgtaat aagtgcaaat gcaggaccag
     2701 ttacaattcc tgtacctgga ggtggtttta cttgggacaa taccattaca aattggtaaa
     2761 taaacccatt gctttagtta tgggtctagt gttagttctt ttcttaacta gttgttcaaa
     2821 aaaacaagaa atacaaaaga ctgatctgat tccttttcta ggaacttggc aaactaaaga
     2881 tcattcagaa cgtctagtaa tcactaattt aaatacttca aaaaatatag aacaatatac
     2941 catcttagta aaagagaaca atcataaaat tcaaatcatt gataaaaatt taaatataaa
     3001 tgaacaagtt ttcatttaca aagcaacaaa taatttacta acactgcgct ctaataaaaa
     3061 acaattagat tacactttta gtttggattc tccaaaaaag ctcgtattta ttttgacaac
     3121 gaatgatggg acagaaggga cagctaaacc aattgaattt tttaaagctg attgatgaat
     3181 agttgtttga tattacaaaa caataatttc accgaaggtg aaacaagcga aactttacaa
     3241 aaagtggcta gcatttgcta gccttttttg tattgtggag cgtgggtttt gagggggatt
     3301 caagggggag cttgcgcccc tttgaaaagc ggttatttgc accaaatacg taggtatttg
     3361 gctagcaaat atagagcttg ccaaaccctt ttagtgagct ttactcattg taccttttta
     3421 ggtacaatgg tattgcgaac gatacgtccg taggacgtac tgaaagggtg ggacttatga
     3481 gcgaacaaaa tcaaaatttg gctagcgatc attctaaaaa atatacctat cgatccgagc
     3541 caaaacaaat cagttttcga gtgagcgaat ctgaatttgc aaagctgaag cagtcagctg
     3601 aagctttgca aatgagtgtg ccggctttcg taaaagccaa ggcacaaaac gcgcgtctgg
     3661 tgacaccgaa agtggcgcca gacattgccc aggcaatcgc gcgcgattta gccaaagctg
     3721 gagggaatat caatcaaatt gctaagtggt gtaatacaca ccaacacgac gttgctcctg
     3781 gagacgccca acgcttatcc gaaaacctaa aaatcatgca aaaggagcta caaaagatat
     3841 ggcaacagtt aaagtaagcc gaaccacttc ttgtagtcgt gcaattaatt acgctgaacc
     3901 acgagcaacc attaaaacag gaataaactg tgacgtcgat tatgccaaaa gtgaaatgaa
     3961 acaaattcgc atgctgtatg gaaaagacga ccgtgtacag gcacacttat tgattcaatc
     4021 ttttcgacca ggagaaatta ccgctgaaaa agctaatcaa ctaggggcag agtatgcaaa
     4081 aaaaatagcc cccgatcacc aaatagctat ttacacacat acagacaaag accatattca
     4141 taatcacatt gtcattaact ccgttaatct ggagactgga aaaaaatttc aagctcatgg
     4201 tcaagcattt ctaaacaaat gttatgacct aaatgacgag atttgtcttg cacataactt
     4261 accaatcact gagcgaggaa agaaaccaga aaaacgaacc atgtctgaaa taaaattaaa
     4321 agaaaaaaat gaacctgttt ggaaagacga aattcggatt gcgatcgatc aaacaatgaa
     4381 aaacaaaaaa accagaacct atgaccaatt ttgtaattcc ctcaaaacat tggggattca
     4441 ttgcttcaat cgcgggaaaa atttcactta cgaattgatc cacaaaaaga aaaaagtacg
     4501 atccaacaga ctaggaaagg actacgaaaa ggagacgatt ttacatgagc tggacagacg
     4561 agaacgaacc tataaccaac a
//
LOCUS       DACDYM010000127         4522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDYM010000127 DACDYM010000000
VERSION     DACDYM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.133"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..1661)
                     /gene="rny"
                     /locus_tag="IWM59_002594"
     CDS             complement(105..1661)
                     /gene="rny"
                     /locus_tag="IWM59_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9980576.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(2072..3121)
                     /gene="recA"
                     /locus_tag="IWM59_002595"
     CDS             complement(2072..3121)
                     /gene="recA"
                     /locus_tag="IWM59_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9980577.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3230..4471)
                     /locus_tag="IWM59_002596"
     CDS             complement(3230..4471)
                     /locus_tag="IWM59_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9980578.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT         1167 a         1018 c          789 g         1548 t
ORIGIN      
        1 atcttgagat gttgaataaa aaataatgat agcaatgttt tttcgtgcct tacaaaaaaa
       61 gaagagctgt ggcttttgcc gcggctcttc ttttgtattt tttattattt agcataatca
      121 gttgcacgag tttcccgaat aacgattact ttgatgtgtc caggataatc cagttcttct
      181 tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt ctaaatccga gatttcttct
      241 ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga ttctacaccg
      301 gcgaagctgt tcgaaatatt ttccagactt cttaaacgac ggatgtagtt ttctaatgat
      361 tcacttcgcg caccaggtct tgctgctgag agcgcatctg ctgcagctac aaggacagag
      421 ataacagaag tagcttccac atctccatga tgagaagcga tcgcgttgat tactactggg
      481 ttttctttgt acttggcagc taattccgca ccgatctcaa cgtgagatcc ttcaatctca
      541 tggtcaagtg ctttcccaat atcatggagc aacccggcac gttttgctaa ttggatatct
      601 tcgccaagct ctgctgctaa gataccagct aattttgcaa cctcgaccga atgcttcaag
      661 acgttttgtc cataactagt tctgaatcgc agacgtccga tgatcttgat tagatctgga
      721 tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg aatacgttca
      781 tccatttctt tacgagattt ctcgaccatt tcttcgatac gcgctggatg gattcgtcca
      841 tcttgtatca gtttttccaa ggtcatccgt gcgatttctc ttcggattgg atcgaatccg
      901 gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat cgataccagt cagcgtctca
      961 agcgttcgaa tattacgacc ttctcgtcca atgatacgac ctttcatttc atcattcgga
     1021 agggtaacga ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg ttgaatcgct
     1081 aatgataata agtttttcgc tttgcgatct gcttcttctt tggcacgctg ttcagattct
     1141 tttaccatta gagttaactc atgattcagc tcttcttcag tcgatttcat gatgacatct
     1201 cgtgcgtcct cttttgataa accagcaatt ttttctagtt ctttttgttg atcttcaatc
     1261 attttttcaa cgtctttttc tcgctcttca attaattgct gttttgaact aagtgcttct
     1321 tctttacttt caagtgagtt ctcgcgtttt tctaacgagt catctttacg attcagcgtt
     1381 tgttcgcgtt gaatcaaacg attctcttga gattttaatt ctaacttact ttctctcagc
     1441 tcactttcaa cttcagaacg gtatttctga ttttcttcct tagcttctaa caatgcttct
     1501 ttttttagag tttctgcttc tttttttgca ctatcgatga ttccagcagc tgatgattgt
     1561 gcaccagcta tttctttctc gtgacgtgac tttgcgacca taaaccctaa accaagaccg
     1621 acaattaaac cgatgatagc gaggagaatg ttgaatacca taaaatccac ctccatacta
     1681 tctttttcat ttttatcatc tatacagctt ctatgataaa ttattctaaa ctcgtcaata
     1741 tgcatcttcg catatatgtg tacaaaaaac atgcacaact ttattctaat gtttgtaccc
     1801 cgtagtgtca actttaaata aacaaagaaa cggctttcta cttattcatt aaacgatggt
     1861 ttttaaaata aatgacacta gtttcatata ggtaaacctt taaattgaga aacttttcta
     1921 ttaaattatt ctttgtagat gaacagatat ttttatttta tgtacccact cgtataatta
     1981 aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag tacctactct
     2041 tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc aattcttctt
     2101 gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct gctctaacta
     2161 gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta gcgttttccc
     2221 gtccttgacc aatccgatct tctttataag aataccaagc accgctttta tcaacaatgt
     2281 ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa ccatacatga
     2341 catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact ttgatctttg
     2401 tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt acttccaaac
     2461 ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca
     2521 tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt gttttgttga
     2581 tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg
     2641 agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact gaatcgacta
     2701 ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga ccttgttctc
     2761 ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt tgtgcatatt
     2821 gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt ttttgtactt
     2881 ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca tatacttcga
     2941 tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca agagagccac
     3001 ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata gagcctttac
     3061 catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga tcatctgcca
     3121 atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc ttatcatatc
     3181 ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat cattttctta
     3241 aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg atagcagcac
     3301 gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc
     3361 atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg gtgaaagcta
     3421 cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc acacattctt
     3481 tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa ccagcttttg
     3541 tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca gcaatgtcgc
     3601 ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca gtctgcttgt
     3661 tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg tatccataaa
     3721 aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct tgattccctg
     3781 cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt ttggcatatg
     3841 gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg
     3901 attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca gaaggaaaac
     3961 gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca ttaggcggac
     4021 cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa gcaagccctg
     4081 tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga ttgtttttgg
     4141 tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct tccgtatttt
     4201 gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg
     4261 gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga gcaatcaact
     4321 cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg atacctagat
     4381 cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct agtaaaagtt
     4441 ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata tagtatacgc
     4501 tgtattttta tattttttta tt
//
LOCUS       DACDYM010000128         4480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDYM010000128 DACDYM010000000
VERSION     DACDYM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.134"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..2008)
                     /locus_tag="IWM59_002597"
     CDS             complement(59..2008)
                     /locus_tag="IWM59_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9980579.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="IWM59_002598"
     CDS             complement(2050..3108)
                     /locus_tag="IWM59_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP9980580.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="IWM59_002599"
     CDS             complement(3227..3682)
                     /locus_tag="IWM59_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980581.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..>4480)
                     /locus_tag="IWM59_002600"
     CDS             complement(4266..>4480)
                     /locus_tag="IWM59_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9980582.1"
                     /translation="RMTNIDKRILEQAKEEFLAPDENGIQVFETFDYEKIYAKKGRRV
                     DRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1292 a          888 c          635 g         1665 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat
//
LOCUS       DACDYM010000129         4475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDYM010000129 DACDYM010000000
VERSION     DACDYM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.135"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            321..1052
                     /locus_tag="IWM59_002601"
     CDS             321..1052
                     /locus_tag="IWM59_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9980583.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="IWM59_002602"
     CDS             1197..3224
                     /locus_tag="IWM59_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9980584.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="IWM59_002603"
     CDS             3332..4429
                     /locus_tag="IWM59_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9980585.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          925 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gttgg
//
LOCUS       DACDYM010000130         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDYM010000130 DACDYM010000000
VERSION     DACDYM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.136"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IWM59_002604"
     CDS             574..1008
                     /locus_tag="IWM59_002604"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980586.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IWM59_002605"
     CDS             2298..3221
                     /locus_tag="IWM59_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9980587.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IWM59_002606"
     CDS             3237..3776
                     /locus_tag="IWM59_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9980588.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DACDYM010000131         4424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDYM010000131 DACDYM010000000
VERSION     DACDYM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.137"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWM59_002607"
     CDS             690..1658
                     /locus_tag="IWM59_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9980589.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWM59_002608"
     CDS             1669..2484
                     /locus_tag="IWM59_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9980590.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWM59_002609"
     CDS             2552..3262
                     /locus_tag="IWM59_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9980591.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4424
                     /locus_tag="IWM59_002610"
     CDS             3298..>4424
                     /locus_tag="IWM59_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9980592.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1490 a          661 c          921 g         1352 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaa
//
LOCUS       DACDYM010000132         4416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDYM010000132 DACDYM010000000
VERSION     DACDYM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.138"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            59..913
                     /locus_tag="IWM59_002611"
     CDS             59..913
                     /locus_tag="IWM59_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9980593.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="IWM59_002612"
     CDS             1010..3259
                     /locus_tag="IWM59_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9980594.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKIPEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="IWM59_002613"
     CDS             3262..>4416
                     /locus_tag="IWM59_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9980595.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          626 c          806 g         1428 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga taccggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DACDYM010000133         4324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDYM010000133 DACDYM010000000
VERSION     DACDYM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.139"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..281)
                     /locus_tag="IWM59_002614"
     CDS             complement(<1..281)
                     /locus_tag="IWM59_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9980596.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..1272)
                     /locus_tag="IWM59_002615"
     CDS             complement(730..1272)
                     /locus_tag="IWM59_002615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980597.1"
                     /translation="MKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGN
                     DFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADL
                     QVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTL
                     FYRQNVSQRKLERSEDEIEW"
     gene            complement(2049..2239)
                     /locus_tag="IWM59_002616"
                     /pseudo
     CDS             complement(2049..2239)
                     /locus_tag="IWM59_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2359..2721)
                     /locus_tag="IWM59_002617"
     CDS             complement(2359..2721)
                     /locus_tag="IWM59_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980598.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="IWM59_002618"
     CDS             complement(2725..3033)
                     /locus_tag="IWM59_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980599.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="IWM59_002619"
     CDS             complement(3042..3461)
                     /locus_tag="IWM59_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980600.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="IWM59_002620"
     CDS             complement(3454..3687)
                     /locus_tag="IWM59_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980601.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="IWM59_002621"
     CDS             complement(3684..>4324)
                     /locus_tag="IWM59_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9980602.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DACDYM010000134         4282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDYM010000134 DACDYM010000000
VERSION     DACDYM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.140"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..1257)
                     /locus_tag="IWM59_002622"
     CDS             complement(37..1257)
                     /locus_tag="IWM59_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9980603.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IWM59_002623"
     CDS             complement(1277..1882)
                     /locus_tag="IWM59_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9980604.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IWM59_002624"
     CDS             complement(1931..2863)
                     /locus_tag="IWM59_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9980605.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IWM59_002625"
     CDS             complement(3284..4144)
                     /locus_tag="IWM59_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9980606.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DACDYM010000135         4136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDYM010000135 DACDYM010000000
VERSION     DACDYM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.141"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(55..249)
                     /locus_tag="IWM59_002626"
     CDS             complement(55..249)
                     /locus_tag="IWM59_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980607.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(754..939)
                     /locus_tag="IWM59_002627"
     CDS             complement(754..939)
                     /locus_tag="IWM59_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980608.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(1789..2130)
                     /locus_tag="IWM59_002628"
     CDS             complement(1789..2130)
                     /locus_tag="IWM59_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9980609.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(2130..2360)
                     /locus_tag="IWM59_002629"
     CDS             complement(2130..2360)
                     /locus_tag="IWM59_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9980610.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            2647..2985
                     /locus_tag="IWM59_002630"
     CDS             2647..2985
                     /locus_tag="IWM59_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980611.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(3190..3429)
                     /locus_tag="IWM59_002631"
     CDS             complement(3190..3429)
                     /locus_tag="IWM59_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP9980612.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(3405..3752)
                     /locus_tag="IWM59_002632"
     CDS             complement(3405..3752)
                     /locus_tag="IWM59_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9980613.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         1357 a          763 c          614 g         1402 t
ORIGIN      
        1 caacgggttt tcagaaaaaa atgaaatgaa aaagatctct tacggcaatc tattttattt
       61 ttttgatttt ttataaatgg aaaatgcttc aagaagtcca agaaataaag ccgtataaaa
      121 acaagaacct atgaaatctt tatttattag atattcaaca gaaatcccga taacactagc
      181 caacaatata tttatcagaa gtgtaaaata aggatgcttt ttatagaata gaactagtgt
      241 tgttttcata gactctcctt tcttcatatt tccaattaat cacaaattca cggcattact
      301 agaaaaatta aaggggcgca gaagtggaaa aatactcttc gactttccct acaaaccagg
      361 ccatcttgtg tttaataact ataccaaatc taaggtacct ctaaatctaa attccatttg
      421 tggcggtgac ctccaatttt taactcttct atctcccatc cagtatacat aaccttgata
      481 cttcgaccac ttatatatcg aacacacaaa tacctatcca ttgcaggagt actacctaac
      541 ttagttgcta cttgcatcta aataacagct gaaaagatag gacttactct tagtaaagta
      601 tctgaactat taatttttaa actatcattt ttatcctcaa ctacatgcac atctgttgca
      661 acttcacttt ctgtctcatc cagataaatg attaaggaat cgggaattgc aatatcttca
      721 gctgacgctg gaatagttga gctaaattag tcatcatatc tttattataa aataaccaac
      781 agcaaaaaat acaatgaaaa acaaacttgg aactaatgta tcattaataa atgttgaaaa
      841 attcagcaac tcattactgt atgattttag cagcccaacc aacatatacc ctataaaagc
      901 taataagatg gttaggaatc ctttagaatt tttactcatt catagacact tccttaaata
      961 acttttatta tagaaggtat tgtaaataat aatattgaca aaaagtgact caaaataaaa
     1021 aaaaacttac attatactgg aatgaactgt attgatagtg tacctaaaag ctctactgac
     1081 ataagatcgt gtaatcggta aagtaatttt attaattata cgggatgtaa tcaattagag
     1141 aataaactaa tcataaaagg tacttttaca ttcataattg tcgctttgag attctacttt
     1201 ggttcactat ctgacccaat tgcacatttt cgatcattac aagtattggt gtcgttagaa
     1261 caactaaata aaaactagcg gtagattttt cagaatctat gctaatttct gtatggatgt
     1321 tccagtgatg gcaaatttct cttttcattg tttttcttgt cgcatgatat gcctttttgt
     1381 gttattcagt taaactatag aaattctttc ctagacagat cttgatccca aattttacgt
     1441 ttttgagcat cgatatagtt taaaatgaca ttactcatat ctaaagagtt tagatttttt
     1501 aggactaaaa ctgtcagttg ttctttttct tctttctgtt aaagtattgt cgcgactttt
     1561 cgtatacgtt aagatatgtc gtgaaaaaac aagttttgtg acacaaaaaa acgaatgact
     1621 tgaggaaatc acctcaatca ttcgtttaaa atgctatata ttctttagtt taagatacgg
     1681 aaattaatgc catcgaccac actagtagaa tgaacgacat ttacttggcc tttttttaat
     1741 ttcgttatgt ttagcatata aaaatattat agagtttact tacaatgctt aaaacatata
     1801 ctgtataatc tgatcaattt tagccatgtc ttgcaatggt aagttttcta tttttttgag
     1861 ttttcgttcc ttaaaatcaa gagattttaa ttgtgtaata attacttgtc cttttatatc
     1921 tagctcttca ggtaatgagt aacgtgtcgg taaattcttt atagtggaag taattggaca
     1981 tatgaccgaa aacattgttt tacggttaaa ttcataacga gagacgacta gtcctggtcg
     2041 tcttttttga atttcttttc ctacggaggg atcaaaatca atccatacaa tatcaccttt
     2101 tttaggaata taaattcttt cttctgacac tataaaattt ctctcccttc aggagttaaa
     2161 tcttcccatt cgtctttctc ataatactcg gcctcttccc caccactaaa tggatcttca
     2221 atttttggaa ctaacgtaat tgttccatca tcagaataca caacgatata ttcttggttt
     2281 tctgaaggtt tctttccgtt atcagaaggt aaagttacca ctacagaact tccttgtaat
     2341 ctagattttg taacaagcat tatcatcact cccttaataa aagtatacta tataacacta
     2401 agtaacgcaa aattcccctt tgtaaactat ctagaaaatt gttttgttta aagtatatct
     2461 tattggatat aaatagaaat aaattgtagc cagttcattt tttctttaac taaaaaaata
     2521 atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag ctaacccccc
     2581 gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac aacaggccat
     2641 cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga caaatttcaa
     2701 ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat caaagaatgc
     2761 ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt tgctattgta
     2821 gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc aactcatatc
     2881 aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac tgccaaccag
     2941 tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata aacatcgtta
     3001 ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta tacattagaa
     3061 aggtcgactg tgaccctata gatgcagtat atgcagctta tgagtatttc ttctgttctc
     3121 tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt ctttgaaaaa
     3181 tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa tgatttttcg
     3241 cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga caagcggcat
     3301 tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga ttgattctgc
     3361 cattttttca tcctgatggc tacgataaaa accgccaata tacactattt tttccttatt
     3421 tgaaaacata aatttgtctt tttcttcatt ccactcattg ggaaaacaca acgattttgc
     3481 aaaaatggtt gatgaaaggt ctttttttct tctactgtct ctgcacgggc attaatcatc
     3541 aattgtcctt ttttaaatcc ggtaaatccc cacttcatga tttccggagt gaggatgttt
     3601 tcttcctctt ttcttaacgt cacaacaaga tttgacggaa aaacttctcc agctgctaat
     3661 tctgctgcct gcttcggttg tttcgtctgg gcttctcgat aataattctg aaggagctct
     3721 gactctagat caaaaaaata acgaccacac atcctaatcc ctcccaatct ttttttctat
     3781 catacactgc aacaagaagt tcgcaccaac aaaacgcttt caatttttgt agaactaaaa
     3841 aagttgtgac aagtgacaaa ctctccagtg aatggtattc tcctgccaac tcctcggtca
     3901 caagtcacaa tattcaacaa acatgagaag ctgctttttg aaattgttcc ttgtgactcg
     3961 gagtgaaacg gcaggcttac gacctctggg taaacggtgt tcaaaagcca gtttctcggt
     4021 attaagccaa atttatgcaa aaatgtagaa agctattttc acaaaatttt tcttaatact
     4081 cgaaactaag cgactttttc acaacctctt ccttaaacgg tgttcaaaag cttgca
//
LOCUS       DACDYM010000136         4129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDYM010000136 DACDYM010000000
VERSION     DACDYM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.142"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(173..523)
                     /locus_tag="IWM59_002633"
     CDS             complement(173..523)
                     /locus_tag="IWM59_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980614.1"
                     /translation="MIFENESEEIGRMTEENKVDLLETLVINKKEFEQLFLDFFVQYD
                     RIYDFNSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDADYYQPQEVFR
                     EFRNKIIGFSQTTK"
     gene            complement(564..1058)
                     /locus_tag="IWM59_002634"
     CDS             complement(564..1058)
                     /locus_tag="IWM59_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980615.1"
                     /translation="MFKVYFPFQWLVKGIFFVLLGLILFGSYLTIKARYFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESGKSETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFNL"
     gene            complement(1051..1353)
                     /locus_tag="IWM59_002635"
     CDS             complement(1051..1353)
                     /locus_tag="IWM59_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980616.1"
                     /translation="MSDPYIKVYCSKETKKLFDSFYEKNKGTLGTKANMFRVMVSNLP
                     MLASQSNKKFSDPESIKLEQTISELESSIMKKLDGIDQKLSCSLKTKYKTEEKKDV"
     gene            complement(1376..2713)
                     /locus_tag="IWM59_002636"
     CDS             complement(1376..2713)
                     /locus_tag="IWM59_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9980617.1"
                     /translation="MGSIIKIASKSRSIHNTISYMLNKQEDSELVTGIGVSNDLNAIE
                     EFKRTNEIFGDKGKIKLWHFTQSFDPLEDISAEKAHELGVKWAEKMFPNQSILVVTHT
                     DKGHVHNHFGISPNDQRTGKKMDIRKNDIRVLQAWNDNILIDNGINPLDMYKYNHLVA
                     DRENWKEREFRTENKISNREKIKLAVSEVAQYAKNYDDFHAELKKNHSIETYRFAKNQ
                     RIGYKLLDSEDGLILKIGGKRLGQNYDMNYLDDLFTENKKAEQIQSEEVLTEIAIRYL
                     GKSLNGRADSRFNWQERAQRFELDNENIPFLRSSKDYEEFYDKYKFEIEDRIENLLDV
                     FGENKNQFFGFAVEEEPLKYKKKCVNLVYKKVVYDIAESLSTDYDQFNIQDTEPALKR
                     NFKKRYIQETKRVLYLFNRAIDDGTIEKARREYEETARGIDYQKQQNQYDMDF"
     gene            complement(2698..3195)
                     /locus_tag="IWM59_002637"
     CDS             complement(2698..3195)
                     /locus_tag="IWM59_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980618.1"
                     /translation="MAIDVSKLPLEERRKRYKRNEDSRRQNRPIKFRVDDYQYQELEK
                     TADIFGLSVPMYVKKIAVDNAGKSLKYTDRQYKEINFHLLAIGRNLNQLVKTANYLRV
                     QLNKKDTEIQKEEHQSVYLSFTTKQERLKRLYAEKNDLMIQIKSISDPLNRLEKELNK
                     LWARL"
     gene            complement(3344..3682)
                     /locus_tag="IWM59_002638"
     CDS             complement(3344..3682)
                     /locus_tag="IWM59_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980619.1"
                     /translation="MARKKQKSIADLKKELAELERKLYPELGKYVYEKTGISSVKEFE
                     ETFEINQKGTQEKWQKAVNFANSEFTLVHEFLASDQLMETKFQQFLRRKGETAYRIFY
                     REEAQEESVR"
     gene            complement(3686..3871)
                     /locus_tag="IWM59_002639"
     CDS             complement(3686..3871)
                     /locus_tag="IWM59_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980620.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     VMKNELMNELSHIQKGA"
     gene            complement(3885..>4129)
                     /locus_tag="IWM59_002640"
     CDS             complement(3885..>4129)
                     /locus_tag="IWM59_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980621.1"
                     /translation="SASNQPRTVEVRETRAEASQGDKTQKINIVEERKERPNEKVIVE
                     QEVKEKKRQRFTYENNPLFQDISDKPNPLFDSLFKK"
BASE COUNT         1163 a          827 c          522 g         1617 t
ORIGIN      
        1 tgtgtccaag atattgactc aaatccaact ttacctatat tttctttgaa gtttgttgga
       61 gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa gcgcccatca
      121 aataaagcaa gcgttgagga ataattctat caacacttgc tttattaagc tttcattttg
      181 tagtttgact aaaaccaata atcttatttc taaactctcg aaaaacttct tgtggctgat
      241 aatagtctgc atctatttca aatgtttctg agaataattc attgaatttt tctactgaaa
      301 taatgatttt accattcttc tctcgactgt tttctattat ttctgcttct aattcaaata
      361 aatctagtga cgaattaaaa tcataaatcc tgtcatattg aacaaaaaaa tcaagaaaaa
      421 gttgctcaaa ctctttctta tttatcacta gtgtttcaag cagatcaact ttattttctt
      481 cagtcattct tccaatttcc tcactttcgt tttcaaaaat cacaaaaatg atattaactt
      541 aaaagccact aacaaaataa gacttacaaa ttaaagggta atccaacaaa ataaccattc
      601 gctctacgta ctacgatttg gtagtcctgc ctttcaatcg gtaattcata atagtaattt
      661 actgtttctg atttaccact ttcaatatac tcaggattat ccaatatcat agcatactca
      721 taaagctctt caccatcctt attttcaatc gaccattgtt gcggacgtat cggattttct
      781 gtatcatttt tcattgtata ggtcacttgt aatacacgat catgctgtgc aaattcattt
      841 cttttttcgg tcagttcaat atttttaaat gttagttcaa ccccatctgc ctgataagtt
      901 tcacctattt ttaaatcttt tgttacaatt tttattggct tttcttctgg aaaatacctg
      961 gctttaattg ttagataact tccaaaaaga atcaatccta gtagcacaaa aaaaattcct
     1021 ttaacgagcc actgaaacgg gaagtaaact ttaaacatct tttttctcct ccgttttgta
     1081 tttggttttt agagaacacg ataacttttg atcaatacca tccaactttt tcattatact
     1141 tgattctagt tctgaaattg tttgttcaag tttaatgctt tcaggatcac taaatttttt
     1201 atttgattga ctagccaaca ttggtaaatt tgaaaccatc acacggaaca tatttgcttt
     1261 cgttccaaga gttcctttat tcttctcata aaagctgtca aacaattttt ttgtttcctt
     1321 tgaacaatat actttaatat acggatcaga cattcttttc cctctccctg tttacttaaa
     1381 aatccatatc atattgattt tgttgttttt gataatcaat tcctcttgct gtttcttcat
     1441 actctctccg agctttttca atcgtaccat catcaattgc acgattaaat aaataaagaa
     1501 cacgttttgt ttcttgaata taacgcttct taaaattacg ttttaaagcg ggttcagtat
     1561 cttgaatgtt aaattggtca taatcagttg ataaactctc tgcgatatca taaacaactt
     1621 ttttatacac caaatttaca catttcttct tatattttaa aggttcctct tccactgcaa
     1681 atccaaagaa ttggttttta ttctcaccaa aaacatccaa taaattttct attctgtcct
     1741 ctatttcaaa tttatattta tcataaaatt cttcataatc ttttgatgat ctaagaaatg
     1801 gtatattctc attatctaat tcaaagcgtt gcgctcgttc ttgccaatta aatctactat
     1861 ccgctctacc atttaacgat ttaccaagat atcgaatagc aatttcagtg agtacctctt
     1921 cagattgaat ttgctccgct tttttatttt ctgtgaacaa atcatcaaga taattcatat
     1981 catagttttg ccctaaacgc tttccgccaa tctttaagat taacccatct tcagaatcta
     2041 agagtttgta cccaatacgc tgatttttgg caaaccggta agtctcaata gaatggtttt
     2101 tcttcaattc cgcatgaaaa tcatcataat ttttcgcata ctgagcaact tcagaaacag
     2161 ctagttttat tttttctcga tttgaaatct tattttcagt tctaaattca cgttctttcc
     2221 aattttcacg atctgcaact aaatgattat acttatacat atcaagtggg ttaattccat
     2281 tatcaataag tatattatca ttccaagcct gcaaaactcg aatatcattc tttcgaatat
     2341 ccattttctt tcctgttcgc tgatcgtttg gcgaaatacc aaagtgattg tgaacatgcc
     2401 ctttgtctgt gtgtgtgaca accaaaattg actggttagg aaacattttt tctgcccact
     2461 taacacccaa ctcatgtgct ttttcagcgg aaatatcttc tagtggatca aagctttgtg
     2521 taaaatgcca aagctttatt ttccctttat caccaaaaat ttcattggtt cgtttaaatt
     2581 cctcaatagc atttaaatca ttcgatacgc caattcctgt cacgagttca gaatcttctt
     2641 gtttatttaa catatatgaa attgtattat gaatactcct actttttgaa gcaattttta
     2701 taatcgagcc cataacttat tcaactcttt ttccaagcga ttcaatggat cacttattga
     2761 ttttatttgt atcattaagt catttttttc agcatatagc cgctttaatc gctcttgctt
     2821 agttgtaaaa gataagtaaa cactctgatg ttcttctttt tgtatctcgg tatctttttt
     2881 atttaactga acacgcaaat aatttgctgt tttcactaat tgattcaaat ttcttcctat
     2941 tgctagtaaa tgaaaattaa tttctttata ttgtcgatcg gtatatttta gacttttccc
     3001 tgcgttgtct actgcgattt tcttcacgta cattggaaca gataaaccaa aaatgtctgc
     3061 tgttttttct agctcttggt actgataatc gtctactcga aactttatcg gcctattttg
     3121 tcgtcgacta tcttcattac gtttatatcg ctttctcctt tcttctaatg gtagtttact
     3181 gacatcaatt gccatgcgca cttctccttt gcaatattat ttttaagttc aaaagaagca
     3241 agcgagggga aagggaaccc ttccccgctt gtcgagggag taaactccct acgagcaccc
     3301 tcatgctccg cattttatcg agattagata gccgataaat cagctaccta acactttctt
     3361 cttgcgcctc ttctcgatag aaaattcgat acgctgtctc tccttttctt ctcaaaaatt
     3421 gttggaattt cgtttccatc aattgatcgc ttgcaagaaa ttcgtgtact agggtaaatt
     3481 cgctattcgc gaaatttacc gccttttgcc atttttcttg agtacctttt tgattaattt
     3541 caaaagtttc ttcaaactct tttactgatg aaattcctgt tttttcatag acatactttc
     3601 ccagttcggg ataaagtttt ctctccaact cggcgagttc ttttttcaaa tctgcaattg
     3661 acttttgttt tttccttgcc atttttcatg ctcctttttg tatgtgacta agttcattca
     3721 taagctcatt tttcatcact aattcttctt gaatcatcag ttcaatataa tttgtgaccg
     3781 tgattttttc atttgtcgct ttctttactg agtcgcataa ttcggcaagt tctaagaaat
     3841 cctgcgtact aatctgtaga attaattgca tgacaatcac ctctttattt tttaaataga
     3901 ctatcaaaca gcggatttgg tttatcagaa atatcctgaa acagtggatt attttcataa
     3961 gtaaatcgtt gacgtttctt ctccttcact tcttgttcta ctatcacttt ttcattaggt
     4021 ctttcttttc gttcctcgac aatatttatt ttttgcgtct tatctccctg acttgcttca
     4081 gctcttgttt cacgtacttc taccgttctt ggttgatttg acgcactac
//
LOCUS       DACDYM010000137         4101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDYM010000137 DACDYM010000000
VERSION     DACDYM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.143"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1614)
                     /locus_tag="IWM59_002641"
     CDS             complement(<1..1614)
                     /locus_tag="IWM59_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9980622.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFK"
     gene            complement(1636..2229)
                     /locus_tag="IWM59_002642"
     CDS             complement(1636..2229)
                     /locus_tag="IWM59_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980623.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(2230..2562)
                     /locus_tag="IWM59_002643"
     CDS             complement(2230..2562)
                     /locus_tag="IWM59_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980624.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(2640..3182)
                     /locus_tag="IWM59_002644"
     CDS             complement(2640..3182)
                     /locus_tag="IWM59_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980625.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            complement(3243..>4101)
                     /locus_tag="IWM59_002645"
     CDS             complement(3243..>4101)
                     /locus_tag="IWM59_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980626.1"
                     /translation="EDLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAV
                     KEGLKDAYGEGQVNAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEG
                     QHGQLPKTDPEQYRYKSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEK
                     STKIGLPKTQNLPMKDSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNL
                     AESGETENASVQNIVTKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELK
                     EQLLKDIKP"
BASE COUNT         1069 a          835 c          665 g         1532 t
ORIGIN      
        1 tttgaagtct tgttcttcca tttgtttttc tttaattaag cgtcgttgtc ttcgtccttg
       61 aatcaatgcc tgattccaaa tcaacgtcat ggccatgtac atctgacatt gttgtacccc
      121 tcgatctagg cctgtaatag agtcaatgtc aaaaaagacc aactgttgtt ggtcaaggtg
      181 tcgaatcgta ctatgaccat caaaaatcat gccataagtc tcacataagg attccacgat
      241 agactccatt tcttctttcc gattcttttt ctcttgggaa gcccctggta gttccagttg
      301 tcctaaaaag gctaaaaatt ctttgaacgt cggataagct tctggatctt tccctgtgac
      361 ttgcgtatca gtatttgaac gcgaatttga ggaagtaaag ccgcgtggta ctgttccata
      421 ctcttcataa aaacgattta agtaggtctt aaattctgct ttcatcgttc cttttagctg
      481 aggactcact aactgtacct gatagaccac tttgtctaag tgtgtttgat aggacgctgc
      541 ttcattgatc ttcaaatctt tgactgatcc atcgtcatat aaactggatg cttcatcata
      601 cgtccgtgtg gcaaatatct ctaaaggatt gatcattccg tcagatccgt ctaaacgcac
      661 cataataccg ttatattcct ttaagaaagg aataaagtct tttccctttt caaagcccca
      721 aataaagtct cctgcagcta aatgggcttc cgcgatcatt ttaagaacgg tcgatttacc
      781 agctcccatc attcctagaa gcaaggtatt ggtagataaa cgtacactat ttttatacgt
      841 ggcattccag acaaatgggc cgcctgtcat ggtttcccca tattgtgtgc ctcctggatc
      901 ttttagcgtt tggtggtgga acgggaatcc tccgccaatg tttaacgatg gaagtgattg
      961 ccctttgata tttccaaacc attcagcttg ttcatcgaac atggtaaaca tcgattgcca
     1021 atctcgtttg gttttatgca gataagtcac cgcacgataa tccatccctt ttagattttc
     1081 gatcagttcc tgcgcccgtt catccacttc ttggatcgta tttccgtaaa catgtactct
     1141 cactttgact tgtttagaaa tttccccatt agaagtcaat tttcgggcat attccgctag
     1201 ttccaggtat tcttccgcac ttttatttcg gtcagtcgct tttcgttcat tatctgatcg
     1261 atcatttaat tcatctaatg cgcggttgat ttgatcaatc actttttgct tttcatctgt
     1321 gctaacatct agtgtggcca ctgtatgagg attgttcatg agattggcca accaaaaaag
     1381 agtaggattt tttgtgtaat gataaactgt cacacatcgt gaataaccat ttccataggt
     1441 cacatattct tcatcaaaat tcaacccacc ttgtggcatc acttgtttta agaaatctaa
     1501 atcataacct tttgatttta aaatctctat ttcacgatta ccaagttgtt ttaatggatc
     1561 aatcaccttt tgattacttt gcgttgaaaa agctgttttt atcacatcta gcatgaatca
     1621 tttcctccat cttttttatt cgtcattcat gttgttcatt cgataaagaa tcttaatcaa
     1681 ttcttttttg tagagtggtt ttaaccctaa aatgccacta gatcgcatta actgacgatt
     1741 tctagtttgt aaatccttga ttgtctttcc ttcaataacg aaaaagaagc ttaagtcagg
     1801 acgttcttgt tctgcccgtt ttaattcctg gatctcctct actaatttct tttcatattt
     1861 tcttaatcgc tgtagatttc ttcctagtga ctgttccata cgatttttat ctaataactg
     1921 ccgcttttgc acgatttgtt ccgttagatc cgtctctgtg gtcaaactaa ctaacttaat
     1981 tgttggcaca tacgtacgca tgacggtggt caactgattc attcgttcta gtttctcttg
     2041 ttcatttaat ccctccacgt cagtggaaac gacacgataa taacgctgaa aatgatcttc
     2101 tctagtaatt aagactccct cttctgtaca ggatttatat ggaaataagt ccaagataga
     2161 gcgctcttct ggttttccaa actgcaaaat ggatttctta tcaactgaat tttgtccttt
     2221 gactcgcatt tattcatcct ccttatacgt accatgataa tttcggttag gatctaacgc
     2281 atgatagtgg ttcttatccg taaagaaaat agattcgata atcgcaaaaa tttggcgttg
     2341 accagggtta ttatagggtc gccaaacaat cactaacgct atcgcaagcc ataatccata
     2401 attcaagagt gcaaaaaaga gtggtaagtg gacaagatta ttgactattt tcgcccaaaa
     2461 aaagcagcca ccaagcgtta atagatctaa aaagaaaaag gcgaaaaact tccattcccc
     2521 actaaatctt ggaggatttt tcatatgaat gtatttatcc ataatataac catacccctt
     2581 atattttgta tttttagaaa taaaaaacac tgtgatttct cacagcgttt ttctaaatat
     2641 tacaactcta attcatttcc taaatcacga gaacgatttt ttcctcgtcg taattgataa
     2701 ttcaattgac gattttcttc ttttaattca acaatttttt gttccagatt tcttttcttt
     2761 tgaattaaat cataattttg tgcgcgtaat tcttcatttt ccaaatgaac tcgttgcatt
     2821 tcatcgttta atcgatttac attctccaat gattcttgta tcgtttcttg cctaaaatta
     2881 agtgagccaa gttgacgatc tttttgttgt ctttgctctt tcaattctga cgatatttgc
     2941 tcaacagaat gaaatatttc cgttaatttt actgtgaatg tgggcatagc atctttcaaa
     3001 taagtgaatc cttctgaaag gtgtacaata atgctttcgg tatgtgtttt ttgaccatct
     3061 aaaatagact gatgatcttt ttgtaaacta tccgtatctt taaatccttt aatagtcacc
     3121 acaaatatcg ttaaaaatgc tcccagtgca gttataaacg tagctattaa aatatcctcc
     3181 acaattttct cctttccatt ttaaacttgt tttataaaga aaaaggccct taaataggcc
     3241 ttttatggtt tgatatcctt caatagttgt tctttcaatt cattgttcct gtttttagtt
     3301 tctcttgtca catttatttt tgtcggtgca gtaaatgcta cgtttgctgg tctgtatcca
     3361 ttatgaactt tattgattgg cttaggtgca gcttcttttg tcacaatatt ttgaacggac
     3421 gcattctctg tttcaccact ttcagctaaa ttgtaagaaa cagttgtcag atttccattt
     3481 tcaggcaaac gacttgattt catttgaaca gtacctgcag gtgattgatg aacaaagctt
     3541 tgatcattct ctttgaattg attcaaacga ctatctttca ttggtaaatt ttgtgtctta
     3601 ggcaaaccaa ttttcgttga tttttcagtt ccaacttcga tgccattatc atttggcaga
     3661 tcgatggaat cctcatccgt ttgtacttct tgatttcgtt ttggtaaatc tgaaatgcgt
     3721 ttttcgtttt cagaattaat gttagattta taacgatatt gttctggatc ggtttttggt
     3781 aactgaccat gttgtccttc ttctgaatct ttttctccga tcgaattttc gttagaatgt
     3841 aactcatctt ggtgatcttt ggctgaagga atcgctgcac cttcttgatt ttctttttca
     3901 cttgttgtat ttgccttcac tgctgcattg acttgtcctt caccatacgc atcttttagt
     3961 ccttctttga ctgctgaagc tttttctttt tgtttttcta gtgcttcgga tggattcttc
     4021 acagcttcag caaattcttt agctctcgtg ccgtctaatt tctcttctaa ggtgtctttg
     4081 gctttttctg taagatcttc a
//
LOCUS       DACDYM010000138         3934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDYM010000138 DACDYM010000000
VERSION     DACDYM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.144"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(319..540)
                     /locus_tag="IWM59_002646"
     CDS             complement(319..540)
                     /locus_tag="IWM59_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980627.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(638..883)
                     /locus_tag="IWM59_002647"
     CDS             complement(638..883)
                     /locus_tag="IWM59_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980628.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1123..1737
                     /locus_tag="IWM59_002648"
                     /pseudo
     CDS             1123..1737
                     /locus_tag="IWM59_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2107..3108)
                     /locus_tag="IWM59_002649"
     CDS             complement(2107..3108)
                     /locus_tag="IWM59_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9980629.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3267..3533)
                     /locus_tag="IWM59_002650"
     CDS             complement(3267..3533)
                     /locus_tag="IWM59_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9980630.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3523..3879)
                     /locus_tag="IWM59_002651"
     CDS             complement(3523..3879)
                     /locus_tag="IWM59_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9980631.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1240 a          713 c          520 g         1461 t
ORIGIN      
        1 tctgtcctct ctgtcttttt tctcaatttt acactaaaat agattttttg gaaaactttg
       61 caacagaacc gtttttacta tatacttctt cccagaactt gtccgtctcc ttcaaattat
      121 ctctacctct cgagttcatt gggtggtttg cccatttatt aaacatggaa tcacctttct
      181 catactcata tatttatgtc ctatttcaat tatatttata tatgcttaaa taaatttgta
      241 tcctgaaatt tattgtaata tgaagctgta ttttacatat agtaaccaaa gagttaataa
      301 aataaaaaga gcgaaccatt atagatcagc tcttttttca ttgctgtttt taatcttatc
      361 tagaatcact tcaaatgttt caaaggtatt tacatctaac tgacataacc aaagcaagta
      421 cttcttttct ttgtaagtcc aatcaatatt acggtaagag gacattagat tatggtacct
      481 tgctctttgt tcaactgaag ccaattccca gcgtttttca aatatttttt cttcttccat
      541 tattgatccc tcgcaagacg ctacctcaaa cttaaataat gggcagcgaa agtctaattc
      601 cttagatcta agtatagcaa acaaatatga aaaaaactta gaaggtagta aaagttcttc
      661 cgtcttttgc tttgcttcta ttcttaatgt gtttcgttat cggtgcccta caaagatttt
      721 cattaattac acactcattt attgttgtgt attttctgac atttctctct tgatcaatta
      781 ttttaattcc atgggcatcc tgatagttca taggagagga gcggctatca gaaatagttg
      841 cacctatctc ttgaacttct tttacaactt ctggaaaatc catccatcct gcttcagctg
      901 tcaaaagtaa taatctataa tatttatctt tccttttcaa tctaagaaat cgtcacccca
      961 ttctttttct tctattataa actatttaac ttttagaatt caaaaaccgt ttgtctattt
     1021 agagtttacc ctaaatagac aaaaatataa cattgaaaaa tttagagttc taacgtatga
     1081 ttagggtgta ttataaacgt tatagaaaag tagggaataa atttgaaaat tgcttatatt
     1141 agagtttcat ccatcgatca aaatgaacaa cgtcaaattg aagaaatgaa aaagtttggc
     1201 gctgaaaaaa tttttatcga aaaacaatct ggagcgacga ttagtcatcg gccgattttc
     1261 caggaagctt tagattttgt cagagagcaa gatattttta ttgtagaagc cattgatcga
     1321 cttggacgca attatgacga gattatcgat tcagtgaatt atctaaagaa gaagaatgtg
     1381 cgacttatta tcaccagttt accgatcatg gctgaagcaa ttggaaatcc tctcctagat
     1441 aaattcatca aagacttaat catttaaatc tcagccatga ttgctgaaca agaacgtact
     1501 gaatcgaaac gacgccaggc acaaggaatt aaaatcgcta aagcaaacgg cgtctataaa
     1561 ggtcgtccta agttatacag tgcagacacc aaagatcctc aacgtcgcct tgtctatagg
     1621 agtatcgttc aggatcttga gaatggggtt gctatctcaa agattgcaac agactacaat
     1681 gtgactaggc aaacaattta ccgaataaaa aaagaaatcg accaactaat tgtatgattt
     1741 cttttttgaa tcacgtaatt attaattagc tttgtcatca atacagacaa tcattttaga
     1801 ttcatctagc taagttgatt acatttatgt taaaaagaat ttagttaaca taaatataat
     1861 tgatgcataa atcgtcctta tttaaaggca tttaacattg acaacttagt ataatgtatc
     1921 ttatgtaaac taataagatt tgaattttat tttatacaca ctcgataata gatcaatttt
     1981 atggtccccc gcatttacga ttgcacaaga gaaacataaa gtcgacctat tagcatttct
     2041 tttcaattca aacattaaaa aagtttatac ctctgctact aaaaaatgaa attatctatt
     2101 taaaatttaa aacggtaaat ctgattcatc caaattggaa aatggatcag cctgtattgg
     2161 agctttgtat ttctccataa taacagatat ctttttttca ttttttaaaa aatacctttt
     2221 aaatttctta tagtcctctg aaatcaaaat atagcgtgat ggatactcat caaaagaaga
     2281 atcgtattct tcctttaaaa ttcccgattt acttaatttt tctagtacat caaatactct
     2341 atcactcaag taactcctta aattgttatc actttcccat tgaactactt ttctataagt
     2401 tgaattatca gttgaaaaga tagaattttc atccataaac gcatatatta gcagcaatct
     2461 ttcatcattg tcaaattcta ttggatcaaa aatatctaag ttgtcatcaa aatctttatc
     2521 agttccggta acttttttgt taattactga aggtgtcttg taagaaatag aatctaaatc
     2581 aaagcgtttt gattctaaat tattttgtgc tttctcaatt aaataattca ctttttttgt
     2641 gagaaacctt ctgatattgt ctttgctata tgttccataa aaacccttat cttctaaaga
     2701 gtccctgtac ttcttaactt ctatatactc ttcagccaaa acttgtggaa ctggttcatc
     2761 taagaaaaaa acccctacct cgcctttgct atttttataa aagtaatcaa tttctgctat
     2821 agttcccgat ctataattat caactggaga acccaactta tggccaaaaa tcgctaataa
     2881 tatatcacta ttttctacaa tttgtttatt aataacactt tgcccagagc tattatttat
     2941 ataagtagga atcgcattcg tttcccatcg aattggcatc aaaattactt ttgtattatc
     3001 tgtattttct atattccatc tatatatggt ttcttctact tcattcctct ttgctgctat
     3061 atcacctggt gatgctataa atacttttat tagatttatg gttttcattt attgtacctt
     3121 tctctttatg tctctttaaa gtaatttttg tataaaattt attgactttt acactatagg
     3181 acaactttta tctatttagt acaaaaaggc ccactaaact aattgattag ttaggagcct
     3241 attacaggta tattttcaac tcgcttttaa gcaaattcta ctaccagctt tttgcctaat
     3301 gccctagcga tatcatcaag tttatcgaaa gatacgttat cccctctctc aattcgagca
     3361 atagtagatt gagttgtatg tgctttttgt gcaagctccg cttgagttag gcctgcttcc
     3421 tcccgtgcat gataaagagc aaccgcagtt tcgagttttc ttccttcacg gtcatatgct
     3481 tctgcaaaat ctttgtcttt ttgcttttct tcaatcaatt ttttaatcat catttgtgaa
     3541 ccactcctct ctaagttctt ttgcatgttt aagttcactt attggtgttt tctccgtttt
     3601 tttagtaaat ccatgggtaa tgacatacct tccattgacc acatggaaat agatagctct
     3661 ttggatgttt gatcctacct tacttcttaa ttcgtacaaa ttagtgtcta attttttcac
     3721 ccatttcaac cgttgagcta ctagcatccc attcttttct gtttcttcta ttgtacgtaa
     3781 aagcttcgca ctatcttttt ttggtaatgt ttccaaccat tctaaaaatt catcatgacc
     3841 attaggtctt ttaaatgttt caaaagatat tttttccata cccaattata tcatatattg
     3901 attttatatt atagtatata acataaaaat tcga
//
LOCUS       DACDYM010000139         3866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDYM010000139 DACDYM010000000
VERSION     DACDYM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.145"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..666
                     /locus_tag="IWM59_002652"
     CDS             1..666
                     /locus_tag="IWM59_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980632.1"
                     /translation="MAYYLLSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQFIRQN
                     LTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVG
                     FILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEV
                     TVKGIRSTLKDGQETKTIDQRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGK
                     VVI"
     gene            710..979
                     /locus_tag="IWM59_002653"
     CDS             710..979
                     /locus_tag="IWM59_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980633.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1211..1507
                     /locus_tag="IWM59_002654"
     CDS             1211..1507
                     /locus_tag="IWM59_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980634.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1509..1931
                     /locus_tag="IWM59_002655"
     CDS             1509..1931
                     /locus_tag="IWM59_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980635.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1950..3542
                     /locus_tag="IWM59_002656"
     CDS             1950..3542
                     /locus_tag="IWM59_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980636.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3563..>3866
                     /locus_tag="IWM59_002657"
     CDS             3563..>3866
                     /locus_tag="IWM59_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980637.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT         1518 a          554 c          758 g         1036 t
ORIGIN      
        1 gtggcctatt acttgttaag tgagccaaaa cagatcgcgg cgtcaaatat tttcacggca
       61 gtaatgacat taatgtgtgt catgtgcttt tctattttta gttacttact cgtccaattt
      121 atcaggcaaa atttaacaat gagtaaactt ttcaaaagtt tattggactt acttatctta
      181 ggcagtatgt taacgactct aacagatcgg ttcgaacaat ctgaaaaaac ggtagagggg
      241 ataaagaact tcttaacgac gattgatcaa tttataaatg gatatatgta tttgtttgaa
      301 gtaggcttta ttctcacttt gtttggttgt gtatttataa aactcattaa aagatcaatc
      361 gaaaaagaaa tggttattga tctaattccg acgctgagcg gctatgagta ttacgttgat
      421 tttggaaagt atgaggagtt gatccatacg gatatcgttc acacgagtat ccatgaagta
      481 accgttaagg gcatcagatc tactttaaaa gatggacagg agacgaaaac aatcgaccaa
      541 agaaaagagg tctacaattt ttataaagtt gaagcaagtc tacgatatgg cattgggaaa
      601 ttgaggttag atgggagtaa tattcaacaa aacaaaccat atgttttagg aaaagtggtg
      661 atttagaact tcctatgaaa gatgaataaa taaggagaat cttttgaaaa tgattcttct
      721 ttacagaatt gtgcttatag ttggcacgga gttaaaaatc ttaaagaagg agcagttatt
      781 caaagtgaaa gaacatcata atcagttact atgtattaaa acgggtacga tgaacattca
      841 ataccgaaca gaatatttag aattaacgaa tcaaactgat tttttgtcga ttcgagagca
      901 acataagcaa gaaaaattaa tggctactta tagccaattg attgatgagt gggaacaatt
      961 caaggaatct aaaacataaa attccattta aacaccttat tttttaaaga gcataaggaa
     1021 acgtgacccc acttttaaaa aaagtggttg gataggctgt gcctatcacg tttctgaaaa
     1081 aaataaatga ccccaaaata ccaatatata ggatagtaca aggatagcaa aggatagtaa
     1141 aaggatagag gtaggatagc gaaaaggata ggatcctttt cactatccta tattatattg
     1201 gaggatattt atgaatgtta aaattagaga tttagatcca aaattcattt ctgaaattga
     1261 tcgaagatgc gttgaactaa gtaatcgtac aggaaataaa tggagtcgta atgattattt
     1321 aaagctactc gttgaaaatg attttgatcg tcctttgatg gaatacaaac aagaaaagtt
     1381 tgaccagtta ttagaaaagt tttcagaaat tcaaagttat aacacaaaga tattagaaga
     1441 atatgttagc caaaataatc gtattataga aatcttgatt gagcaaaata gaggaagtga
     1501 actttgatat ggcaaaggtt atagacaaac acatttactt tcaccaaaca catatcgatg
     1561 tattggatga aactatttct actcatgcag aggttaaaaa ctatagtgaa gctgttcgct
     1621 ttttatgtat gaactatgat ccagaagtgg agaaaaaagt aaattccaat caagcaaagt
     1681 tgaatggaat gagtaaagaa atttctattt taaccgaaat ggtttcagaa tttgcggatc
     1741 ttcatttgaa agatacagga atattaattg gtaaaaattc aactgtttat caagaagcaa
     1801 aagaacgagt ggaaggaaaa attaaatcca atcaaacaaa aaaatattcc cctcagttga
     1861 agaaacctgt gaatgaatct gttcatgcca gagtagatcc atttgatcca acgaagattc
     1921 taaaaagata aaaatgaagg aggataataa tgactcaatc gccttcagtt attctcactt
     1981 gtcaatttac tttgccgaat aaaaagtcat tttctactta tattgactat attacaaggg
     2041 aacaggctct tgaagaaaag gaacgacgta cgcccgaaga agaacaggaa cttcaacgaa
     2101 tcaagaatgc tttagaggag ttttatattc ctgaaggaga aactttttcg gagagtaaga
     2161 cgggtaaaga attcagtgaa acgtcgaatg aagctcaatc gttgatggaa catggaatca
     2221 attttgacaa gttagaggaa caagatttta ccaattatct ttcttatatg acacgtcact
     2281 atgcgttaga acaaaagaaa gaactgacgc aaagtgaaca aaaagaatac caacgcttga
     2341 accaagcaat gaaaaaatat caaacagaac cccataaaaa agcttcagga aatcttcctg
     2401 gagttttttc tatagcttca gacgaagtga aaggaagtga tctgaaggag attaagcaaa
     2461 tcttccaaag aggacaacaa aaaggctcaa tcatttatca agatgttgtt tctcatgata
     2521 attcgtatct tgaaaaaata ggattgtacg atcccaaaac agatacttta gatgaagaag
     2581 ggttaaaagc agctggaaga aaaatgatgg agacattatt cgagaaagaa caattgaatg
     2641 aaacaggtta ctggatggca acgattcacc gtaacactaa acatatccat attcattttg
     2701 cagttgttga aaaagaaaat acgcgtaaac cttacacgga ggttgaaaat ggtgtgagtt
     2761 atatcgtacc gaaaggtaag agaagacaag caacattaga cgcaatgaag accaattatg
     2821 tacatgagtt agaacgtttt tcttatgaaa aggatcgaat gattggtatg gagaaacgaa
     2881 atttattgac gcggaaaagt gatttgcgta acaccttaac aaaagtggta aaagagccag
     2941 cgcgttacga tcgacacgcg atgaacttat taaatgaagt atataaaacc ctacctgaca
     3001 aacgttcgga atggaattac ggggatgaaa aacgaaccaa actttcacca tacacaagag
     3061 agaaactcga tgagttaacc aaatatgttt taacaaaaga acctgaatat caagaatata
     3121 tacagatttc aaaagcattg aaagaagaag caaaaagtct ttatggggaa agcaagagag
     3181 aatcaaagga tgcagagaaa aatgctctct ttgatttaaa aaagcgcaca ggaaatgcca
     3241 tcctaacaga gctgaagaga caggatagtc aaataaaacc attaatagaa agtgttgatc
     3301 agatgcaatc tatttacaat gaacgatcct ttcgaacgct taaacaattg ccaacgaatg
     3361 aatacaaata tagttacgaa gaattcatga agcgcaggaa acaattccaa caaagagttg
     3421 tcacgcagcg gagtttgcgt caattaaatc gaacgattaa tgcccatgaa gaaaaatacc
     3481 acgcaagtaa agcatacgaa gagcaacaac gaagaattca aatggaacaa gaaagacagt
     3541 aaagacagta ggaggaatag caatgaaaca atatttagat caatggaaag ttatagaagg
     3601 atcattaaga gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag agcatttgtt
     3661 ccagataaga gaaatgttga gaaacgaaca gtttgatcca aatcaatttt tagtcgttga
     3721 gtacccagct actggtgtgt attgttgtaa tcatgtgaat ggtgaaaaat attttatcat
     3781 tcaagaatat gagggcaagt tagcacctta ttatactact tgggaaatga atgaagaggg
     3841 aatcaataac tttccatgta aaagca
//
LOCUS       DACDYM010000140         3818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDYM010000140 DACDYM010000000
VERSION     DACDYM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.146"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1032
                     /locus_tag="IWM59_002658"
     CDS             <1..1032
                     /locus_tag="IWM59_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAP9980638.1"
                     /translation="VKYWKRGDIDTATQLTNTFTNTIYYDANASDNKYFNENAQKAVN
                     ALIFALLEQADQTGDYSKLTPNNLVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLP
                     PGNIAKKQYGATKIGSDKAKGNILSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKY
                     LDLKLHESLLNQRVVVEFYHKGKKFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDH
                     QGKEYVAWYEFSQRVLKDEEGNIVYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIR
                     MHYTDKPMAIFMLTPDYDPSNHIIVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDE
                     FGNSVTCLA"
     gene            1727..3643
                     /gene="ltrA"
                     /locus_tag="IWM59_002659"
     CDS             1727..3643
                     /gene="ltrA"
                     /locus_tag="IWM59_002659"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9980639.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
BASE COUNT         1442 a          607 c          779 g          990 t
ORIGIN      
        1 gtgaaatact ggaaacgagg agacatagat actgcaacac agttaaccaa tactttcaca
       61 aatacgattt attatgatgc caacgcttca gataacaaat actttaacga aaacgcgcag
      121 aaagctgtca atgcgcttat ttttgcgttg ttagaacaag ctgatcaaac aggagattac
      181 tccaaattga caccgaataa tctcgttgag cttctttctg agattggcgg ttttaattat
      241 tatccagatc cagacaatga attcaaacag ttgaatgcct tagatgaatt tatgaatcag
      301 ttgccacctg gaaacattgc aaaaaaacaa tatggggcaa cgaaaattgg atctgataaa
      361 gcgaagggaa atattttatc aacggcaatc acgggattga atccatttac tctaacgaaa
      421 attgcaaaga tgacttctca atcaacgatt gattataaat cggttgggtt tccaaaatac
      481 ttagatttga aattacatga aagtctttta aatcaacgag tcgtagtaga attttatcat
      541 aagggaaaga aatttcatga agaacgtgtc aaagtaggct ataaaggatt tagtgaaatc
      601 aattttgatt gtcatttagt tgcaggtgat caagtttgtt taaaatacga tcatcaagga
      661 aaagaatatg tggcttggta tgaattttct caaagagtat tgaaagacga agagggtaat
      721 atcgtttatc ggaaaaaacg aggagaaacc catttgccag aattagaaaa agaagccgtt
      781 ctaacattag atgaggaaaa aagtaactta ttagtagagt ctattcggat gcactatacc
      841 gataaaccaa tggctatttt tatgcttaca cctgattatg atccatctaa ccatatcatt
      901 gttagtattt ttcttagtca gttatataaa gaattatcta cgcaatgtgt taagactaaa
      961 ggagataaat gtcaccgcag gattcatttt attttagatg aatttgggaa tagtgtgacc
     1021 tgtttagcct aaggataaaa gaggctagtt ccaacgaaca ttggaataac tttactatcg
     1081 agaaatcaaa tgacggggta acgccctgaa gggtttccac tgatactgcg acatgcgtta
     1141 gtaggatgtg catccaaacg cgtatgaagc tcgctgaagt cggcagaagg gtaccgtaag
     1201 gaaagacacg atacgtatct accacgcaag tgctctagag gtagggtatg tacctgatat
     1261 gtcgggtgtc ctaaaaagtt caataaggtg agaatgattt gattatctga caaacttgcg
     1321 aatggaatgt ctgaaacgaa taactcatag aaatgtgagg gggaaggggg atacgtcctc
     1381 tttccattga ctcgaggttg agtaaaaatg tgtttctatg aaaaaccttg catggttaaa
     1441 ggctatggag tagtcaacag gcaaaagcaa gcacctaagt tgaaatctat ggatagatag
     1501 taaggaacag gggaagcatc tagcacggag ggagcatacc gaagatgtgt ttctaaggaa
     1561 aaaccgaaat cggttataac ttaggtgaat agatgtgaaa gtagagccac gaccagtgaa
     1621 aatttcgtaa tgaaattgga ggaacaggct caagtcgtta tttataatat aagaaaccta
     1681 tttaaaatca tggattctcg taagactaaa aaggtcacga accagaatgg agcgtgataa
     1741 aaccatgact aacacaaaag gaattagctt aaagaacaaa caactcagac acgccgaata
     1801 ctacgacatg actgagactt tcgatgaatt gcacgcaatg agtgttgcaa aatcaaattt
     1861 taaaaatctc atgaaattca ttattgatga caataatatc tttctagcgt atagaaatat
     1921 caaaaagaat aaaggaagtg caacggccgg atacgatggt gtcactataa aagacattga
     1981 aaaattggca tgtggtgact ttttaactat tattaaaagg cgatttaagg attacagacc
     2041 aagaaaagtt aaaagagtag agatcccgaa accaaacgga aaacttcgac ctttaggaat
     2101 tccttcaata tgggacagga tagtccagca atgtattcta caaattttag aaccaatatg
     2161 cgaagcaaaa tttaataagc atagctatgg attcagacca aattgttcta cagaaaacgc
     2221 catagcagat tgtcttgcta gaatcaatca aagtaagatg aattatgttg tagatattga
     2281 tataaaagga ttctttgacg aagtaaacca taccaaactt atgcgtcaaa tatggacaat
     2341 ggggattcga gataaacaac tccttgtaat tattcgtaag atgttaaagg ctccgattgt
     2401 tatgcgaaat ggcaatgttg tttttccaac caagggtact ccacaaggag ggattttaag
     2461 tccactgctt gcgaatatat gcctgaatga atttgattgg tggatagcta atcaatggga
     2521 ggaacgaact gtcaaagagc tgaaacacca ctatcgaaaa aacggagcat gggataaagg
     2581 aaatatctat tcgaaattaa gaagccacac aagtttaaaa gagatgtatc tggtacgcta
     2641 tgcagatgat tttaaaattt tctgccgaac aggagaagaa gcccaaaggg ctttttatgc
     2701 cacacaaaaa tggctcagag aaagattaaa gctcccaatt tcaatggaga aatctaaagt
     2761 tacgaaccta aataaagctg aaagtgaatt cttgggtttt tcaataagac tccacaaaaa
     2821 gggaaaaaag aaagtggctt tcacgtacgt ttgtcgcaaa gcagaagaaa acattaaaaa
     2881 taaactaaaa caacagatta aaaaaattca atacgctgga gcaaaaaaaa ataggcaccg
     2941 agcaataggt gactacaaca tgatggtcat cggtatccac aattactatc ggatagcttc
     3001 acattgtagt gtcaatttca ctgagattta tagaagtgtt aggacagtaa tagaagcaag
     3061 actgaaagct agaggaatga cgaaaaatgg aatctatgga caagatcatg aaggataccg
     3121 tccgtatcta aagtctaagc aaatgagata tattgaaggt tttccgatac ttccaattgg
     3181 ctatattaaa aaaagaactc cactcaacaa gcggagaaat ctaaataaat ataccaagga
     3241 agggagaaag ctcattcatc aagagcaaca agctatagaa agctggaaaa tcaagtatct
     3301 tgtaaaaggt tttagtaaca ataagaaata cacggtggaa ttttctgata acaaaatatc
     3361 taagtttatt tcccaaaaag ggaaatgcgc tatcacagga aacgaacttt tgatggaaga
     3421 aattgaatgc catcacatta acccatatca tttgtccaaa gacgatagtt acaagaacct
     3481 tgtcattatc tctagagaaa tccatagatt aatccatgca actagtgaag aaactattct
     3541 gaaactgctt cggttactga aactaaatca ctatcaaatt agaaaactaa acacgtatcg
     3601 aaaaaaagca gctaattttc caatatcaat caaaatgaat tagataggaa acaaattata
     3661 ttgtaattaa cgatggaacg ccgtgtgcgg tgaaagtcgc acgcacggtg tggaccgggg
     3721 gaaaagacgg agataatatc aaactcttac ctatcggaat tgccaccaat ggataatatg
     3781 gaaggaatca tgacggtcac agctggccga aacatgtt
//
LOCUS       DACDYM010000141         3537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDYM010000141 DACDYM010000000
VERSION     DACDYM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.147"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            280..1107
                     /locus_tag="IWM59_002660"
     CDS             280..1107
                     /locus_tag="IWM59_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980640.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            1117..1365
                     /locus_tag="IWM59_002661"
     CDS             1117..1365
                     /locus_tag="IWM59_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980641.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            1396..3507
                     /locus_tag="IWM59_002662"
     CDS             1396..3507
                     /locus_tag="IWM59_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9980642.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
BASE COUNT         1307 a          548 c          678 g         1004 t
ORIGIN      
        1 agttaaagca gaagaattac aaaaaaatta tttagattat ctcatgcctg aaaaagacca
       61 agataggatc acgttaatga catgcacgcc aaaaggtgtt aactcacacc gctttttagt
      121 gtatggtgaa cgagtttcct tcagtaaaga agatttaaaa ctacagaaaa aagcaaaaaa
      181 ataggaaatc aatttcattg gattggcgat tagcactgct attcgctttt tttgcgttcc
      241 tattattttt atttataaaa aaataaggag gagatcggaa tgaataagag aaaatggctg
      301 tttgtttgtg tagcagctct ctttgcaact gcagcttact cacagatatc ttttgctgat
      361 agcagtacta ctccaactac cagtcagaca acagatagtt caacaactgc aacaagtgac
      421 acggacagtt cgaagaacac tgcagaagaa acggcagatt caagtacgag ttctactgaa
      481 tcagcggaat caacgtcaga aagttcttca gagacttcag aaaaactgca agaagtagaa
      541 gatacgcaag taatcaagca aaacattcat attcaaaaaa ttatttcttc gaatcgaatc
      601 aacaatcaag gaaggcaagt gactcagcaa gaaggtgtca atggtgcaaa gtttattgtc
      661 tacgatatca cgaatatctt agaagaaatg acacaagatg ataatgagaa aaatcaatcg
      721 gtagaaataa ttgagtcaaa attgaaagat cgagcaaaaa aactttcata cgatcagttg
      781 aaaaaagtga ctgaaggaga aacccaaaca attgatgatc aggaaggcgt gattgatttt
      841 tcaattgaag tacctgcaaa caaaaaacaa gcatactata tcgtcaatga atcaagccct
      901 gaaaacattt caaatagcga agatattgtt ttattgacac ctgtttcaga tgaaaagggt
      961 gaatttttga cggatgtttg gttatatcct aaatcaaaga aatcagagcc gaaagaagaa
     1021 attaaaaaag ttgtatcaac tggtgtaaag aaaaactttt ttgataattg ttgggatttt
     1081 ttcactcatt tattttttaa cgattaggat gaaatgatga aaataataaa aatgttagtt
     1141 tttaatctat cttttttagc cgcaacaata ttgtgttgcg gtttagtttt ttctcaatta
     1201 tcaatttacc caagatatca acgattgttc gttcattttc taaatatcca gcatgcttcc
     1261 atgccactag aagtgattaa caagctctta atcaatccaa tttttatggc tagtttgttt
     1321 cttcttgcag gattggtaac gtgggtatta ggaaaaaaag aatgagaagc ttacaatcgt
     1381 ttaacaaaat aatagatgga gggcattatg gaaaataaaa ttcattgcac tacgggaaga
     1441 gtatttactt tagtgttgtt tttgttcctt cttctttttg caaggggaac tgcagtccag
     1501 gccttagaaa aagttgatcc gagtcaaatc aaagaaaata ataatctggt actttatcat
     1561 gatagtgatt cattgagaaa tatcgaagta actgggatca atggatttaa gatactagat
     1621 gagtattcaa ctaaaaaaca aaaagtactt gcttggccaa aagtcaaaga tttgaaaagc
     1681 acgtcaaaaa ttgttgccca ttatcaaaag gtgggcactt atctaggaaa agaaattgat
     1741 tgcgacgtaa ctttttctaa ttttgtagcg cacgctgggg ttaaccaaga cggattagct
     1801 aaaacaatta atgggaaaaa gtattataat tatttgctct ttgataaaaa catttatcga
     1861 ggctttttca tgcagtcaat caacgttatg aatgttaaac tagattttaa gtatgcagga
     1921 tcaaaaacaa aagtagcttt gagtgaacca cagccagaaa ttaattcaaa aatgagatcg
     1981 atgagagcag ctggtattga gttgaatgat acatttctat ctatttacag tttaaatgga
     2041 cagtatcagc aacgtgtgtc ttggtataat gacaataatg aatctgtcgg tgaacgtaat
     2101 attgatatga gagaatttgt tgggtacgaa aaaatggatc gcttacctta ttttgtcaca
     2161 acagatacag catgtgcaga atacaaagct cctgcgttat caacaaacaa tttaacttca
     2221 aaagtagtgg gaggacgtgc agaaaaggct tatagctcga atgatcattt caccgatgtt
     2281 gtaggagctg atacttatca cagaagtggt gtaacgtata cgcttcaagg cgcttcccca
     2341 acattcatga ttggcgcaaa tacgaatagt atgatgttta gctttgatac tgcattgctt
     2401 tggacaccac aaccatcgaa gcctacaaaa gaagtgttta acaaagctaa tactgaagag
     2461 gcagcacaca atattgacaa aaaagtgatt ccacaaggat cagatgttta ctatcatatt
     2521 caccaaaagt ttgatgcatt aacagtcaac acaatgaaca aatacaaatc atttaaaatc
     2581 actgatacct ttgacagcaa aaattttgat atggtatcgg atgggaaaaa ctatgatggc
     2641 gcattgtggt atgttgaagc gaatgggact tggaaaaaag ctgatacagg aggaaaatta
     2701 actgtatcag gtaatacagt cacttatact gcttctgaca gctttttagc taatgctgca
     2761 aattacggta gggactacat tttgcagctc catatgagag caaaaaatga tgcaaaagga
     2821 attgcaccaa atgtggctac tgtaacgttt aacaatgatc cgaacaaagg aacacaaaaa
     2881 acaaataaag tagaaaacta tttctctctt aaaccaaata aaaaagtgga acaagatggg
     2941 aaagatgttg atggtcgaaa tgatggtaaa aaaggaacgc ctacagctcc tttaaatgca
     3001 ggatctgaag tacaatatct agtcacacaa aaatggcata caaaaggtgt agacattgcg
     3061 ctcgatcatt acaagcaatt tagtattcaa gatccagtgg aagctcgttt gacgtataaa
     3121 gaaggttctg cgcgagtgat agatatgagt acaggaaaag atattacttc agaaggtata
     3181 ctaacctatg atagccattc aagaacgctg aaatgggaag cttccgctga tttcttaaat
     3241 aacaatcttt tagatggacg agaaattcaa ctgatattta cagctaaaac tcctttgaat
     3301 gaagaattgg atatcgacaa ccaagcaaaa gtttcagtag aaagtattga gaaagcaact
     3361 aatgtagtaa cgattggggt aaaacctaac ttacctcagg tcattgtgcc aaaaactgga
     3421 tcgaatcatt tagtgctaac tgttattgga agtatattat ttcttacttt agcatcattg
     3481 ggctatgtag ttttaaaact caaataaata taaaaacaag gctgattttc agccttg
//
LOCUS       DACDYM010000142         3522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDYM010000142 DACDYM010000000
VERSION     DACDYM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.148"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            130..951
                     /gene="racE"
                     /locus_tag="IWM59_002663"
     CDS             130..951
                     /gene="racE"
                     /locus_tag="IWM59_002663"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9980643.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            954..2309
                     /gene="rph"
                     /locus_tag="IWM59_002664"
     CDS             954..2309
                     /gene="rph"
                     /locus_tag="IWM59_002664"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9980644.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            2310..2828
                     /locus_tag="IWM59_002665"
     CDS             2310..2828
                     /locus_tag="IWM59_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9980645.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            2935..3435
                     /locus_tag="IWM59_002666"
     CDS             2935..3435
                     /locus_tag="IWM59_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9980646.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         1199 a          595 c          800 g          928 t
ORIGIN      
        1 aagtcacaac cgcttttcct ctttccattc gaaaaaaaga gatttcgatt gttttaagca
       61 agtaagagca cttgaaaatt cacgctaaaa agtacacaat agggaggata gaatttattg
      121 aaaaaaagga tgatacgatt gacagataat cgccctatcg gatttattga ttcaggtgtc
      181 ggcggcttga ctgtagtaaa agaagccctg aaacaattac cgaatgaaaa tattttattt
      241 gtaggagaca cagcacgctg cccatatggc cctagacccg cggaacaggt aatccagtat
      301 acttgggaaa tgacggatta tctggtggag caaggaatca agatgctggt gatcgcctgc
      361 aataccgcaa ctgcggtggc tttagaagaa atcaaagctg ctctttctat tccagtcatc
      421 ggtgtgatcc ttcccggtac tagagcggca gtaaaaaaaa cacaaaataa acaagttggc
      481 attatcggta cgattggtac ggtaaaaagt caagcttatg aaaaagcact gaaagagaaa
      541 gtaccagaat tgactgtgac aagtcttgct tgtcccaaat ttgtttcagt tgtcgaaagt
      601 aatgaatacc attcatcggt ggcgaaaaaa attgtggcag aaacattagc tcctttaacc
      661 actaaaaaaa tcgatacatt gattttggga tgcacccatt atccattatt acgccccatc
      721 attcaaaatg taatgggaga aaatgttcaa ctgatcgatt ctggagcaga aacagtaggt
      781 gaagtatcta tgctgttaga ttatttcaat ttgagcaatt caccgcaaaa tggtcggaca
      841 ttatgccagt tttatacaac tggctctgcc aaacttttcg aggaaatagc tgaagactgg
      901 cttggaatcg gacacttaaa tgtagaacat atcgaattgg gaggaaaata aggatgcgtc
      961 atgacgggag aaaagcccaa gagcttcgca aactggagct tcaaacaaat gttttcaaat
     1021 atccagaagg atcggtagtg atccgttttg gtgatacaac agttatttgt tctgcaacaa
     1081 tagaaaattc tgttccccct tttttgagag aaacaggaac aggatgggtg acagcagaat
     1141 atagtatgct gcctcgagca acgagtacga gaaatcgtag agaaagcagc aaagggaaac
     1201 tttctggtag aacaatggaa atccagcgtc ttattggtcg ttccttgcgt gcagtagtag
     1261 acttggaaaa attaggagaa cgtagtatca tcgtagactg cgatgtgatc caagcggatg
     1321 gcggtactag aacagcaagt atcactgggg catttgtggc attgaaatta gccattgaaa
     1381 aacttttaag agaaaaagaa ttgtcagaag atccaataaa agaacatctt gctgcagtta
     1441 gcgtaggaat cttaccagat gggacttgcg tcacagattt ggattaccaa gaagattccg
     1501 cagcacttgt agacatgaat cttgtaatga cagaatctgg aaaattcgtg gagattcaag
     1561 gaactggaga agaagcaacg tttgatggtg aacagttgaa tgaaatgctc ttttttggaa
     1621 aaaacgcgat tgaagatttg atcaaagagc aaaaacatgc tttgctaaca gagtttgctc
     1681 aaaatgatga gcggatagaa gaaacaaaaa caatcatcat tgcgacaaga aatccaggaa
     1741 aagcagaaga gtttcgaaat atgttcaaag aagcgggata tcatgtaaaa acattacttg
     1801 actatcctga attgccagat gtggaagaaa ctggcagcac atttgaagaa aatgcccgat
     1861 taaaagcaga aacgattgca caattgcttg atcagccagt cttagcagat gattcaggtt
     1921 tgaaagtgga tgctttaggc ggtatgccag gaatctattc tgcacgtttt gcaggggaac
     1981 aaaaaagtga tgcggggaat aacgccaaat tattatatga attgacagat gtgccggatg
     2041 aaaaaagaac agcacaattc cattgtacgt tagtattcgc ggcacccaaa aaagacagcc
     2101 ttgtagtaga agcggaatgg cccggtcgag tagcccgtat accatctggc gaaaatggct
     2161 ttgggtatga tcctttattt atcccagaag gtaagaaaca aacagcagca gaattatcga
     2221 gcgaagaaaa aaacaagatc agtcatcggg ctcaagcaat gaaaaaattg agtgccgagt
     2281 ggaaacaatg gctggaagga gagagataga tgcggtattt agtagtaagt gataatcatg
     2341 gagacagaga tatcctcgtt gacttagtag atcgttatcg ttctgaagtg gatatctttt
     2401 tccattgtgg tgattcggaa ttagaagctt ctgacccatt atgggataca ttccaagtgg
     2461 ttcaaggtaa ttgtgattat ggaccaggat tcgaacagaa aaaagtgatc caaactggtc
     2521 aggatacagt attcatgact cacggtcatt tatcgaatgt ccgatttggt ttgacacagt
     2581 tagcaatcga agctcgagct gcaactgccg atatggtgtt ttttggtcat acccatcaaa
     2641 taggctgtga gatggatcag aaaatcctgt ttttgaatcc tggtagcatt tctcagccta
     2701 gaggcccgat ccaaatccca tcatatgctc ttatcgaaag tttagatgac caaatttctg
     2761 ttcaatacta taaccgtgcc catcaggcag ttgaagagtt ggccttttct ttttcgagag
     2821 agcgctgaat aaaaaaactc taatttttaa taaaaataag ctttatttta tgaattttgc
     2881 atccggtttc ggtaaaatag tagtgttgaa tgaaatagaa atggaggcca tcagatgatt
     2941 ggacctatag taagagaaat gctgctggaa aatcaggaaa cgtttttgat tcctgcagaa
     3001 aatgtggcaa atgttatgta tcatcatcca ttgtcccatg gtttacttgt gttatcgaag
     3061 gtcggttatt ccaaaattcc tgtattagga aaagatgatc actttgtagg actagtaagt
     3121 ttatcagatg tggtcaataa aatgattgac ttgcaaacaa tcagcatgga cccgttagaa
     3181 ggactaacgg ttgctgatgt gacagaaaca gatgttccga caattgatga aaattgggaa
     3241 ctagaagaag tactccattt gttagtcgat tcgccttttt taccggttgt cacagaagat
     3301 cggattttca aaggaatcat tacaagaaaa gagctgctta aagccgtaaa cttcatggtg
     3361 catgaattag aacgtcgtaa tatattgtct ccgaaaatcg aagaattaaa agaacaaatc
     3421 gatatcgtga gctaaagaac gagaaactaa aagataatca aaaaataagt agaaaaaaca
     3481 gcgtaccagt tttgacttta tcttgaaaaa gaggctggga ca
//
LOCUS       DACDYM010000143         3401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDYM010000143 DACDYM010000000
VERSION     DACDYM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.149"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            120..857
                     /locus_tag="IWM59_002667"
     CDS             120..857
                     /locus_tag="IWM59_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP9980647.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
     gene            complement(905..2083)
                     /locus_tag="IWM59_002668"
     CDS             complement(905..2083)
                     /locus_tag="IWM59_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9980648.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            2106..2189
                     /locus_tag="IWM59_002669"
                     /pseudo
     CDS             2106..2189
                     /locus_tag="IWM59_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            2310..2711
                     /locus_tag="IWM59_002670"
     CDS             2310..2711
                     /locus_tag="IWM59_002670"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980649.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(2918..>3401)
                     /locus_tag="IWM59_002671"
     CDS             complement(2918..>3401)
                     /locus_tag="IWM59_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9980650.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1055 a          626 c          667 g         1053 t
ORIGIN      
        1 aaaagtttga gaatgaagaa agtgagcagt cgaaaaacgg ttgctcactt ttttgcccta
       61 aaatcgaaaa aatataaaaa agtcctttac aacacgtctc aggaaaaact gctaagtcaa
      121 ttctaatatc ttgtggagca agacttgcac cttttgatat aagagagctt agagaactaa
      181 tgagcgaaga tgaactagaa ctcgataaaa ttggagatag aaaaactgcc ttgtttgtaa
      241 taatatcaga tacagatgat acatttaatt ttgtagtttc aataatgtat tctcaattat
      301 ttaatctact atgtgataag gcagatgatg tgtatggtgg aagacttcca gttcatgtta
      361 ggtgtctact tgatgagttt gcaaatatcg gtttgattcc taaatttgaa aaactaatag
      421 cgacaattcg ttcaagagaa atatcggcaa gtataatact gcaagcacaa tctcaattaa
      481 aagcaattta taaagaccat gcagatacaa tagttggtaa ctgtgactct acactttttc
      541 taggaggaaa agaaaaaaca acagtaaaag aattatcaga aaccttagga aaagaaacca
      601 tagacctata taacacatca gaaacaagat caaatcaaaa atcttttggt ctaaattatc
      661 aaaagactgg caaggaactt atgagtcaag atgaaataac tgtaatggat ggtggtaagt
      721 gtatttacca gctaagagga gcaagacctt ttctatctga taaatttgat attacaaagc
      781 ataagaatta caaattgttg gaagattatg ataagaaaaa cttgtttgat gtagaagagt
      841 atttaaaaca aagatagcgt aaaatatttt gtgtaaataa ctcgtccttc tgcaaaataa
      901 tgggttactc agtaaacatt gaagctaatg tatccgtcac ttgttggaag cctttatggc
      961 ttctatttaa gaatttttga ttgtatgtat caaagatgga tactaggaaa cgctctagtg
     1021 attcttcatt ttgaaactgc tcttttctgt ggctgtattt cttgatttgc ttattaaagg
     1081 attcaatcaa gttcgtagag taaatagtct tgcgtatggc taatggaaaa tcatagaaag
     1141 tgagcaggta atcgttcgaa aggatcgatt ccaccatttt tggataactg gttttccatt
     1201 tttccgcaaa agcaccacgt gcttccaatg ccgccttttt agatgacgct tgatacacca
     1261 ttttaaaatc atcacagact tccttacgat catcgacacg cactttgtga ctgatattac
     1321 gggaaacgtg cacacagcaa tgttgaaaac gggctttggg gtagaaccgc tgcacggccc
     1381 ctgccatccc ctttaagcca tccgtgatga aaaggaggac gtttttcacg ccacgttcct
     1441 ggaggtctat taaaatctct tcccaaatcg tgatagattc agttggtgca atcgcatagc
     1501 tcaacacttc ctttgaaccg tccgggcgaa tgccaactgc gatatgaatg gcttccttgg
     1561 cgacggtttt ccgctttaaa gggatatagg ttgcgtccat ataaatagcg gcataacggt
     1621 catgaagctc acgttcctta aatgaagtga cttcttccgt aaatgattta gtcatattgg
     1681 acatggtttg gggcgtgtag tgatgcccat acattttctc aatcaagtct gcgatttccg
     1741 acatggtaat accttttcgg aagaggtgaa tgacggtctc ctctaaggta tcattcgtcc
     1801 gtctataagc aggaacagtc tgttgcttga actcgccgtt gcggtcgcgc ggaatctgga
     1861 gatgaagttc cccgtactcg gtcttgaccg tacggtcata ggagccgtta cgagaattac
     1921 ccgtgttaaa accaatgcga tcgtactttt cgtaatcgag gaaagccgtt aactccgttt
     1981 tgagaagcgt attgacagct ttttccaaat ggacacggaa taattcattc aaatcgcctt
     2041 tattcgctag agtctttaga atttctgtag taaaatcgtt catatggaag tcctcttttc
     2101 tgtgaatgtg ttgtggtaac tctattctac tgaagggact tccttttttc tatttacaca
     2161 aaatatttta cactctcgga ttgaaagaac gcggtctttc aggagtaaag atggtcatct
     2221 cggatgcgca tatagggctc acaaaagctg tgaaagaata ccttctttgt gcgagctagc
     2281 agagatgcca agcacatctc cttcgcaaca tttccgacaa attctccaag aaaatctcaa
     2341 gcgatatcaa agaagacttt aaagccatct tcaaaacggc aagcatcgag ctggctggag
     2401 aacgtaaaca acttattatg gataaatatg gggaagacaa gaaagtcgca actgcctgta
     2461 aaatattgga tgagggcttc gaagatgcca tgcaagtaat ggttttacca gaaaatatcc
     2521 gtcaacgatt gagaacaacg aatgttcttg aaagactaaa tgaagaagta cgccgtcgtg
     2581 aacgtgttgt ccgaattttc ccaaattgtg attctgttat tcgtatcatc ggtgctttat
     2641 tgatggaaaa ggatgacgaa tggacttctg cacctagaaa atatcttgaa tttgatcgta
     2701 acgagattta gcacattaag ccgccaatag tataatatac tggtgagaag tatcaagaga
     2761 cgcttcgctt tttgctcttg acacttctta ccagcatatt ggaccgtagc taggctcaac
     2821 aaggcaaaac atcgtacttt gagatttcct ttttacacaa gattttggac ttgacacagc
     2881 ccctatggtt cttaccttgt ttatgcttat ttaaatctta ctttattaca gtactttcaa
     2941 acttcgtttt gtaagccggg tattttgata tttcataatt atgtccctct ttttgaatat
     3001 attctttgat atctgcttgt tcatctacac tttctttgcg taaaatacgt tcgatcattg
     3061 agcagcagtg catgtaaagc aagaacaatt gattgtttga aaattcgatc cccattagtt
     3121 tttcgagttc tttgacggcc ttttgtacca atctcaaaac cttcattgca tcaaggatcg
     3181 ttaaattttc tacgatcttg ttaatcgaga aattaaagta ttgagcacaa ttataggcga
     3241 taaatggagc atttgattct atcacctgtt gttccttggc gtattcataa attttttctg
     3301 ccaaaaaggt ttttcctgaa ccgcttacac ccatcaataa aatcggtaat ccgttatgag
     3361 gatacaagac agccgtcttg atttgatcga tcgcttcctt t
//
LOCUS       DACDYM010000144         3399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDYM010000144 DACDYM010000000
VERSION     DACDYM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.150"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..276
                     /locus_tag="IWM59_002672"
     CDS             <1..276
                     /locus_tag="IWM59_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP9980651.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="IWM59_002673"
     CDS             419..640
                     /locus_tag="IWM59_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9980652.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="IWM59_002674"
     CDS             656..1498
                     /locus_tag="IWM59_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980653.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="IWM59_002675"
     CDS             1561..3363
                     /locus_tag="IWM59_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP9980654.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1229 a          469 c          703 g          998 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaatag
//
LOCUS       DACDYM010000145         3390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDYM010000145 DACDYM010000000
VERSION     DACDYM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.151"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            647..964
                     /locus_tag="IWM59_002676"
     CDS             647..964
                     /locus_tag="IWM59_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9980655.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IWM59_002677"
     CDS             complement(1055..1420)
                     /locus_tag="IWM59_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980656.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IWM59_002678"
     CDS             1628..2110
                     /locus_tag="IWM59_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980657.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IWM59_002679"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IWM59_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP9980658.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1172 a          519 c          619 g         1080 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac
//
LOCUS       DACDYM010000146         3221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDYM010000146 DACDYM010000000
VERSION     DACDYM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.154"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            301..585
                     /locus_tag="IWM59_002680"
     CDS             301..585
                     /locus_tag="IWM59_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980659.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="IWM59_002681"
     CDS             588..1004
                     /locus_tag="IWM59_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980660.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="IWM59_002682"
     CDS             complement(1141..1860)
                     /locus_tag="IWM59_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9980661.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="IWM59_002683"
     CDS             2535..2630
                     /locus_tag="IWM59_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9980662.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="IWM59_002684"
     CDS             complement(2932..3177)
                     /locus_tag="IWM59_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980663.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DACDYM010000147         3220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDYM010000147 DACDYM010000000
VERSION     DACDYM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.155"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            150..446
                     /locus_tag="IWM59_002685"
     CDS             150..446
                     /locus_tag="IWM59_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980664.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            453..2405
                     /locus_tag="IWM59_002686"
     CDS             453..2405
                     /locus_tag="IWM59_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9980665.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2477..2974)
                     /gene="dfrG"
                     /locus_tag="IWM59_002687"
     CDS             complement(2477..2974)
                     /gene="dfrG"
                     /locus_tag="IWM59_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9980666.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1136 a          530 c          558 g          996 t
ORIGIN      
        1 gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga atttgatact cgttttttat
       61 tgttaaatca acgtttgttg ttaatttaca ccacaaaatg taagttaaaa tgtatataaa
      121 aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg tgtgttaaaa tggttacaat
      181 ttactaagca aaaaggggtg cattttccta tgcaatttct tgagccaaaa aataagaacg
      241 ctaagtctgt ggattgggaa atatcagagc aagtaagggt gatagttaag caatacgctg
      301 aatatgcaga gcgtactgaa agcgaagcag tggatgaatt cttattgaac attttagacg
      361 ataagaagtt tattgaatgg attgccaaca agcgaagtaa taagcggatt gttgaaaaaa
      421 tgggtatcaa agatagagtg ggttgataga caatgaccaa gcttaaaaga ttagccacga
      481 aggaagatga aattgtagat gttaaaattc cgatttcaaa cgaagaatta aaagataggg
      541 ctaaacaata tgatttatta actccaaaac gatttgcaac aaggtataac aaaatgttgt
      601 tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc cattcaatat aattactgca
      661 tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa attcaaagat gtaaaaggga
      721 aaccaagtcg atataaaatg actggtagtt ccaaagataa aggtcataaa ggtatgtact
      781 gcaatgacaa tcctattggc agaggtgtat cgcaaaactg tacagtaacc cctttatcta
      841 attggtctgt tgttgaagaa attaaacgcc ttatagaaat caatagcatc caagatgttg
      901 agccagatta tcaatttcat aaagaaggct gctcggaaga agaatcaacc ccattcaatg
      961 agccgaagca attttacaaa agggggaaaa gtaggggcaa atctcaacgc tatcaatgta
     1021 aggcgtgtaa gaagttcact aatgttctac caaaaagaga agaaaccact acttatcacc
     1081 aacaaaagaa caccattctt ccaatgttcg ctaagatggt tgttggaagg gtttcagtca
     1141 gtcgtacttg tgatatttta ggaattggag ttggaaccta ctatcataag ctggagtggc
     1201 tgtataggcg ttgtttggag tttttagaac gatatgagac acaacccctt caaacaaaga
     1261 aatttaacga gatgtggctt aatacggaca agatgcacta ctacctaaat aacgtccgta
     1321 agaaaggaca aggttcaaag aaatatacgg ggtttgaaga ccttaatatg caaacgtata
     1381 tagttgtatc tgctgaggtc ttatcaagat atgtgttccg ttctgatgtt gcctatgatt
     1441 ggaatatttc aatggatgaa ttaaatgaag atacaagaaa attcaaagaa gaccatctca
     1501 atacttttag tcgtaaaaat gataggttag attggtctta ttatccacaa gaaccatccg
     1561 ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt agggaaaatt actaatagga
     1621 gtagatttgt cgatggttta aatgttgatg ctccttatac tacaactgct cactattggt
     1681 taattaaaca aatggttaat gccgacgaat ggcgaatgat tagtgatgat gacttctcta
     1741 ttagaaacgc cttttataga gtattcacga aagaattaag gttgagcgat gcacatcatt
     1801 ttatttgcca agttaacaag acgaaaagca gaaaacaatg cttaaaagaa ttcggacaag
     1861 caaaagcaga actgctcgat tggggagata tcagaggatt caaaacaaag tttctacgga
     1921 ctttggcatc tcattatcta actgaattac taacaagtca tcaattccac gaagaggcta
     1981 ttagtaaaga tggagaaaga tatcgcaaat atgccgataa ccctatcaaa catccattag
     2041 ccactaagga taaaggattt tattcagttg attgtagaac ggatttatct gctctggaac
     2101 caaacgaaat cgcaaagatg ctattaaatg ttaatgacca ttcgaccaat agctttattc
     2161 aacagataag acgttatata tcatctcttg aaagaccatt aacaacagct cgtggtgata
     2221 agaagagcta tatttacgct aacttcaatc cgaagtatgc tcaatttgcg attacaattc
     2281 taagaactta ctacaacttt tgtcgccctt ttaaatcagc ggataaaaag gttttgacac
     2341 ctgctcaaag attagggatt acagataaac aatttgattg gaaagatata atttatttca
     2401 aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc tttatcttta ctgttcttct
     2461 tcaattaacg cacccgttaa ctcaataagt tttttctttc atatacatga aaatagtagt
     2521 tatacggatt tttatcattc tttatccctt tttgggcaaa tacctcattc cattcctcat
     2581 aattcacttc tggaaaaaaa gtatctcctt cgaattcatg atgtattttt gtgatgtaca
     2641 ttttctcaac ataagggaaa aacaaattat aaatctgttc tcctccgaaa ataaaaattt
     2701 cttcttcgtt tttacataac tcaaaaacat cttctattga atgaacaatt tcacaaccat
     2761 taaaggtaaa ccccttatct ctcgtcagaa taatatttct tctgtcaggt aaggctcttc
     2821 cgattgattc aaggttcttc ctacctaata ttatcggatg tccctttgta gtatttttaa
     2881 catattccca gtccttggga atcctccaag gaatgtcatt ctctttgcca atcactctat
     2941 tcttatccat cgcagcaatc aaagaaactt tcatttataa cacagccttt ttaactaatt
     3001 taccatctaa tttcttattt ctatcaaaaa accttttctc attatcctgc ccgttagttg
     3061 aataagcaat tttttctata agttatatta tcataatacc acaaaatcac aaattttcat
     3121 tcaattatca ttttcaattg cattttataa atcattttgt atgtaatttg gtatgtaagt
     3181 taaaatgaaa aaagtagtcc attttgaagt acattttgtc
//
LOCUS       DACDYM010000148         3217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDYM010000148 DACDYM010000000
VERSION     DACDYM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.156"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IWM59_002688"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IWM59_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IWM59_002689"
     CDS             complement(545..739)
                     /locus_tag="IWM59_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980667.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2091..3131
                     /locus_tag="IWM59_002690"
     CDS             2091..3131
                     /locus_tag="IWM59_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9980668.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT         1193 a          448 c          462 g         1114 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtgatat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg caagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatatta
//
LOCUS       DACDYM010000149         3213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDYM010000149 DACDYM010000000
VERSION     DACDYM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.157"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            21..779
                     /locus_tag="IWM59_002691"
     CDS             21..779
                     /locus_tag="IWM59_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980669.1"
                     /translation="MSFADSSDSSTTQTIDNSITAPSETDSSENTTEETADSNISFTE
                     STNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIISSNQINNQGSQVAQQEGINGAKF
                     IVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKKLSYDQLKKVAEGETKTIDDQEG
                     VIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLLTPVSNEKGEFLTDVWLYPKSEK
                     SEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            1068..3179
                     /locus_tag="IWM59_002692"
     CDS             1068..3179
                     /locus_tag="IWM59_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9980670.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
BASE COUNT         1192 a          495 c          618 g          908 t
ORIGIN      
        1 ctactgtagc ttattcgcaa ctgtctttcg ctgacagcag tgatagtagt accactcaaa
       61 caatagataa ttcaattact gcaccaagcg aaacggacag ttcggagaat actacagaag
      121 aaacggcaga ttcaaatata agctttactg aatcaacgaa tccaacgtca gaaagttctt
      181 cagagacttc agaaaagccg caagacgtag aagatacgca agtgatcaag caagccattc
      241 atattcaaaa aattatttct tcgaatcaaa tcaacaatca aggcagtcaa gtagcccagc
      301 aagaaggtat caatggcgcg aagtttattg tctacgatat cacgaatatc ttagaagaaa
      361 tgtcacaaga tgataatgag aaaaatcaat ctgtagaaat aattgagtca aaattgaaag
      421 atcgagcaaa aaaactttca tacgaccagt tgaaaaaagt ggctgaagga gaaaccaaaa
      481 cgattgatga tcaggaaggc gtgattgaat tttccattga agtacctgca aacaaaaaac
      541 aagcatacta tatcgttaat gaatcaagcc ctgaaaacat ttcaaacagt gaagatattg
      601 ttttattgac acctgtttca aatgaaaaag gtgaattttt gacggatgtt tggttatacc
      661 ctaaatcaga gaaatcagag ccgaaagaag aaattaaaaa agttgtgtca actggtgtaa
      721 agaagaactt ttttgaaaat tgttgggaat tttttgctca attatttttt gataattagg
      781 atgaaatgat gaaaataata agaatattag tttttaatct atccttttta gccgcaacaa
      841 tattgtgttg cggtttaatt ttttctcaat tatcaattta tccaagatat caacgattgt
      901 tcattcattt tctaaacatt caatgtgctt ccatgccatt agaggtgatt aaaaagctct
      961 tgatcaatcc aatttttatg gttagtttgg ttcttcttac aggattggta acgtgggtat
     1021 taggaaaaaa agaatgaaaa gcttacgatc gtttaaaaaa atattagatg gagggcatta
     1081 tggaaaataa aattcattgc actacgggaa gagtaattac tttagtgttg tttttgttcc
     1141 ttcttctttt tgcgagggga actgcagtcc aggccttaga aaaagttgat ccgagtcaaa
     1201 tcaaagaaaa taataatctg gtgctttatc atgatagtga ttcattgaga aatatcgaag
     1261 taactgggat caatggattt aagatactag atgagtattc aactaaaaaa caaaaagtac
     1321 ttgcttggcc aaaagtcaaa gatttgaaaa gtacgtcaaa aatcgttgcc cattatcaaa
     1381 aagtaggtac ttatctagga aaagaaattg attgtgacgt gactttttct aattttgtag
     1441 cgcacgctgg ggttaaccaa gacggattag ctaaaacaat taatggcaaa aagtattata
     1501 attatttgct ctttgataaa aatatttatc gaggattttt catgcagtca atcaatgtca
     1561 tgaatgcaaa actagaattt aagtatgcag gaacaaaaac aaaagtagct ttgagtgaac
     1621 cacagccaga aattcaatca aaaatgagat cgatgagagc agctggtatt gatttaaacg
     1681 atacatttct atccatttac agtttaaatg gacagtatca gcaacgtgtg tcttggtata
     1741 atgacaataa tgaatctgtc ggtgaacgta atattgatat gagagaattt gttgggtatg
     1801 aaaaaatggg tagcttacct tattttgtca caacagatac agcatgtgca gaatacaaag
     1861 ctcctgcgtt atcaacaaac aatttaactt caaaagtagt gggaggacgt gcagaaaagg
     1921 cttatagctc gaatgatcat ttcaccgatg ttgtaggagc tgatacttat cacagaagtg
     1981 gtgtaacgta tacgcttcaa ggcgcttccc caacattcat gattggcgca aatacgaata
     2041 gtatgatgtt tagctttgat actgcattgc tatggacacc acaaccatcg aagcctacaa
     2101 aagaagtgtt taacaaagct aatactgaag aggcagcaca caatattgac aaaaaagtga
     2161 ttccacaagg atcagatgtt tactatcata ttcaccaaaa gtttgatgca ttaacagtca
     2221 acacaatgaa caaatacaaa tcatttaaaa tcactgatac ctttgacagc aaaaattttg
     2281 atatggtatc ggatgggaaa aactatgatg gcgcattgtg gtatgttgaa gcgaatggta
     2341 cttggaaaaa agctgataca ggaggaaagt taactgtatc aggtaataca gtcacttata
     2401 ctgcttctga cagcttttta gctaacgctg caaattacgg tagggattat attttgcagc
     2461 tccatatgag agcaaaaaat gatgcaaaag gaattgcacc aaatgtggct actgtaacgt
     2521 ttaacaatga tccgaacaaa ggaacacaaa aaacaaatac agtagaaaac tatttctctc
     2581 ttaaaccaaa taaaaaagtg gaacaagatg ggaaagatgt tgatggtcga aatgatggta
     2641 aaaaaggaac gcctacagct cctttaaatg caggatctga agtacaatat ctagtcacac
     2701 aaaaatggca tacaaaaggt gtagacattg cctcagatca ttacaagcaa tttagtattc
     2761 aagatccagt ggaagatcgt ttgacgtata aagaaggttc tgcgcgagtg atagatatga
     2821 gtacaggaaa agatattact tcagaaggta cactaaccta tgatagcaat tcaagaacgc
     2881 tgaagtggga agcttccgct gatttcttaa gtaaaaatct tttagatgga cgagaaattc
     2941 agctgatatt tacagctaaa actcctttga atgaagaatt ggatatcgac aaccaagcaa
     3001 aagtttcagt agaaagtatt gagaaagcaa ctaatgtagt aacgattggg gtaaaaccta
     3061 acttacctca gatcattgtg ccaaaaactg gatcgaatca tttagtgcta actgttattg
     3121 gaagtatatt atttcttact ttagcatcat tgggctatgt agttttaaaa ctcaaataaa
     3181 tataaaaaca aggctgagaa tcagccttgt ttt
//
LOCUS       DACDYM010000150         3203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDYM010000150 DACDYM010000000
VERSION     DACDYM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.158"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(20..250)
                     /locus_tag="IWM59_002693"
     CDS             complement(20..250)
                     /locus_tag="IWM59_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980671.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(686..1252)
                     /locus_tag="IWM59_002694"
     CDS             complement(686..1252)
                     /locus_tag="IWM59_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9980672.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1252..2208)
                     /locus_tag="IWM59_002695"
     CDS             complement(1252..2208)
                     /locus_tag="IWM59_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9980673.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2208..2864)
                     /locus_tag="IWM59_002696"
     CDS             complement(2208..2864)
                     /locus_tag="IWM59_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9980674.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT         1015 a          613 c          559 g         1016 t
ORIGIN      
        1 tcattaaaaa gcataaccat tactccgatc tgtttttaaa attagtttct acctctaata
       61 tatatcgagt ttttattttg ccttcagaga atactgtttc tttttttgca gttacaggtt
      121 gtttattttc ggaaaaagct aatatagcta agattgaaac atccatctgg aatttatctt
      181 ttttactgct ttgatcataa aagtctgcat attcatcact gatatttttt ctaataaatt
      241 cttccatcat aaaaatcacc tcaaaacgat tatacgaact tacgttcttg ttgtaaagcg
      301 atatttgagg ggcaaaaaag gggcataatt tgactgatat taagttagct attgatatat
      361 gaatgacata caaaaatata aaatctttta aaaatgctta tatatcaatg tttttgccat
      421 tcttttatat atctgttata tgtcaaaaaa atgtgttcaa tttcaatttg tatttgaata
      481 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaatc aaaaacagct
      541 tctcatgttt ttgatttggt tggacttatt accgcagata taagcttttg agcaccgtga
      601 ataaagaggt tatgaaatca acgctctaac ctgagtatta gagaaaaaaa cgcaaaacat
      661 atcatttatg ttttgcgttt tttatttatc tttttttctc aatacaatat aaaataggcg
      721 gctggttctt ttggttgata aaccgataat tcagcacatt atatgtttcc tgtggcagtt
      781 tggaacaaaa agaatcaacc atatccagtt ctttttctcc accttcatga ccatagtaga
      841 cgacaaggat caatcgtccg cgaggcacaa gtcgtttcaa tatttcttcc atagctgtgc
      901 gtgtagtctc tggcatagtg atgattgctt tatcactttt tgggagatac cctagattga
      961 aaatccctgc tttgagattt tgttgttcag ctatcacttc tcctaacgtt tcgtggcctt
     1021 gtagaaacaa tgagacacgt tcttctaact gacgctgttc tagcctttca cgagtagcat
     1081 cgattgcttg ttgctggata tcaaaactat agacatgacc agtttttccg atgtgttctg
     1141 ctagaaaaac cgtatcgtgg ccattgccca tcgttgcatc tacgacgtga tcccctggtt
     1201 gcaagatttc ttgcagtaat gtatggctga aatgcaaagc agtttgcaac atcagacgct
     1261 cacctttttc cgtacatcat attttccttg gaagctatct ctgcgaagaa gttcttcatc
     1321 aattgcattc aagacttccc attttttcaa gctccacatt ggtccaatca aggaatccca
     1381 cggcgcatcc cctgtcaaac gatggatgat gatctctttt ggtatcattt ccagctggtc
     1441 gcaaatgaca ttgacatagt caggacggct cattaattga aggcgacctt catgatagtc
     1501 ccgcaacatt ctcgttttgc gcatcagatg catcaaatgg agcttgattc cttggatatc
     1561 tgaatctaaa atggtgcgtc tgacattttc tttcatcatt tctaaacttt cgcctggtaa
     1621 tccgttgatc aaatgcgtac aaacacggat attatgtttg cgtaatttag ccacgccatc
     1681 taaataagtt tgataatcat gtgctcggtt gatcagtttt gaagtttctt caaaagttgt
     1741 ttgcaatcca agttctaccc ataaatacat ccgctcgttc agttcagcaa ggtaatcaac
     1801 tacttcatct ggcagacaat ccggtcgtgt accgactgat aatcctacga cacctggtaa
     1861 attgactact tgctcaaatc gttctttgat tatttcaact ggcgcatggg tgtttgtaaa
     1921 attttggaaa taaacgatgt attgttggac atgaggccat ttttgatgca tcatgtcgat
     1981 ttctttctgg aattggattg gaagagggtc ttcaggtgca acgatcatgt ctcccgaacc
     2041 agatacactg caaaaagtac aaccgccatg ggcaacagtc ccatctcgat tcggacagtc
     2101 aaatcctcca tcgatcggca ccttgaaaat tttcccgcca aactcatttc gtaaagcgta
     2161 attccaagaa tgatatcttt tatttccttc tgaataagga aaagtcatta aaagtccttc
     2221 tttctgtaag agttatctat ttgttttctt ttttctctat acactggata agtcaggcac
     2281 agccaagaaa gactgagtaa gtagccgccg atgatatcag taggaaaatg aacgcccaga
     2341 taaatgcggc ttatcccgac acttactata ataaacatca tcagcaattg aagcaatgtg
     2401 cgtaaggttc ttttttgaac aaaagctgga agcagaaata gtaaagaacc gtaaagaatc
     2461 atactgcctg ctgcatgtcc actagggaaa ctgtaacttg tttcaactac taagtgcgcc
     2521 aatgaaggac gctctcgcat gaaaaatagt ttgatcaccg ggttgatgat tccagcaatt
     2581 acaataaatc cagaaacgaa ccaaaatgtt tctgcccttt ttttcttata ccacatccat
     2641 aaagccgcac aagcaaataa aatgatgacg gtaagaggat taccgaattt agtcataaag
     2701 agttggtagc tgttcagatc aggataagaa gctctgacaa tgcctgtaaa ccactgatcg
     2761 aaaccagaaa gccacgacag atggaacttt acgatccagc ccaaaatgaa aaacaggaca
     2821 agaaagaagc taccagccac ctgaaatgat gttttatatt tcatacaatt cctcctttga
     2881 agcaatgact tattatagca tttttggtcc agcgaaaaaa acggtaaaga gataaaacca
     2941 atgtatgatt cctctttgaa taaccaatat tcttataaaa aaaataactg taatcggaaa
     3001 gacatttttt ggtaatgcaa atgaaatgtt agggaaataa aatcagtgtt tttcgaaatc
     3061 cttttaaaca agaacgtaga cgtaggaaga acttgaaaat gaacagaagt tgttcattac
     3121 ggtaaattac aatatgatta caaaaaacaa caaagctctt aaataacttt taataaatat
     3181 taagagaagt tataattatt aat
//
LOCUS       DACDYM010000151         3012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDYM010000151 DACDYM010000000
VERSION     DACDYM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.159"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..265)
                     /locus_tag="IWM59_002697"
     CDS             complement(<1..265)
                     /locus_tag="IWM59_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9980675.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVV"
     gene            450..1337
                     /locus_tag="IWM59_002698"
     CDS             450..1337
                     /locus_tag="IWM59_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9980676.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(1931..2626)
                     /locus_tag="IWM59_002699"
     CDS             complement(1931..2626)
                     /locus_tag="IWM59_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9980677.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(2610..3008)
                     /locus_tag="IWM59_002700"
     CDS             complement(2610..3008)
                     /locus_tag="IWM59_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9980678.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1020 a          569 c          439 g          984 t
ORIGIN      
        1 caacaacgag acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat
       61 cttctattct ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta
      121 aatgtccgac cgtaccggca attgctccaa tccattcact atttccaata aacatatccg
      181 caagtgcatt attgacaaga attcctaaaa caaaaccaac taagactatt aaaacagatc
      241 caatcaaccg aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc
      301 ttttctttct tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt
      361 ttacgaacta agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct
      421 tctgttagac tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa
      481 ttatgtgcct ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa
      541 cgattgcaac gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag
      601 ttaaatcaaa taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg
      661 tatacggaaa agagatttct tcaaaggaag taacaaaaga atataatgaa caaatcaaaa
      721 aactcggtcc aaactacaag gaacaattaa aagcagttgg ccaaacagaa gagacatata
      781 agaaactctt taaacagatg ctggctttcc aatacggttt gaaagccaat gtaaaattaa
      841 cagataaaga tctagacact gcttggaaag aattctatcc agaagttagt acacagatca
      901 ttctattttc gactaaagaa gaagcagata aagcaaaaaa agaagcaaat gaaggagaaa
      961 atttctccaa gcttgttcaa gcttatggaa aaaataaaac attaaaggaa actgatggaa
     1021 aaatgaattt tgattcaacc aatccagaga ttcctactga agtaaaaaaa gcagcattta
     1081 aactaaaaaa cggggaagtt tctgatatca ttcctgtaac agatccaacg acttatcagc
     1141 aaagctatta tctagtaaaa atggtaaaaa aacaggataa aggttctaat aaagacaaat
     1201 acaaatctga gctagaaaaa attgctaccg aagcaaaaac tcttgatacg gaattcatgg
     1261 ataaaacaat ccgtaaagtg atgaaaaaag acaatgtaac tattaaagat ccttatgtaa
     1321 aaaatatatt caaataaaca aatttgaccc aattggggat gtggcaaaat ttgagaagtg
     1381 atttttggaa attccttctt atttttgagg caaaccactt ttgccacaac ttcatcgggg
     1441 ccttttttta tttgtaccaa aacaaatcac tggatttgtt acaatcatga ggatataatt
     1501 taaaaaagtt ttttatttct atcacatgaa tcaatgcgcc attaagtaga ttctttcatg
     1561 cttccctatt ttttcacttg ccgtatcgct tcaaaaacaa ctatcataaa ctcaaactcg
     1621 actgaaaagt tattttaaaa aagactaaaa atatcccttc tggacttgtt cttcttattc
     1681 tgttatacta gagaaacacg aaagatattt ttatgcgaca cttttcattt gtttttatga
     1741 aatttattca aaactggcgt aacttcagag ttacaaggat gtattagagg ttagggaata
     1801 catcgtttaa atgagaaata agtaatagtt agtaatagtc acccaaactt taaattaatg
     1861 aatattgaat cgtcagatag cctcctcgag ttttcgagaa ggctatcgtt tattttatgg
     1921 agaaattgat tcagaagtaa tcagcgacct tttttagttc accaatcgtt tctgcttgga
     1981 aagtttgcac ccaatcttct tggaagcccc tcactgcttt atcaatcgag ggcatctcta
     2041 atacttgatc tagtagctct ttacctaacg ttatcgcatg agctccagat cgaatggctg
     2101 tagtgacttg ttcacaattt ttgaaactag ctgctaatat ttttgttggt ctttgcgtac
     2161 gttcgatctc agtccgcaac gaatcaatca ctttcatagc atcaatgcct tgattttgca
     2221 tcctgttata ataaggtgca atataatctg ctccattatt aatagctaaa tacccttgta
     2281 attctgtgta aatagctgta ccggtaatca aataaccttc ttttttcaac tttttgatcg
     2341 cttgaaggcc tgcttgattc accggcacct tgataaacgt atcttttccc agttcttcta
     2401 tgataactcg agcatcctct atcatttctt cttccgttct tcctattgtc tgaatatgga
     2461 taggaatctg tccgattatt tttttgattt ctttcatatg cgagaagaag tttgacgctc
     2521 cttctttttt cagaattgta ggatttgttg ttacgcctgt taaattcaaa aactgttgac
     2581 aagaataaat actttttgta tttgctgtat caagcatgat ttccatatag ctttccccca
     2641 ttctgatcct cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg catttcctta
     2701 tcttctacat acattgttcc tggaagttcc ggctcatgag atccatcaaa ttttatcgtg
     2761 atatgtccta attcattcaa attagtttgg acaacatctc ctactgctgt gattacatac
     2821 atttgtttat caaaacagag cttcatatca caagttattt cttttcttac ctgattcaat
     2881 tcaatcttat aacaataatc tgttaatgtt tccggtgctt catctccaaa taaaatcatt
     2941 attttttctt tcctgaatac ttctgcttct ataccaacat ctgttacaac cgtttcaaag
     3001 atcgtcatct ct
//
LOCUS       DACDYM010000152         3012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDYM010000152 DACDYM010000000
VERSION     DACDYM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.160"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IWM59_002701"
     CDS             177..359
                     /locus_tag="IWM59_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP9980679.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IWM59_002702"
     CDS             462..746
                     /locus_tag="IWM59_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980680.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IWM59_002703"
     CDS             762..998
                     /locus_tag="IWM59_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980681.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IWM59_002704"
     CDS             1020..1274
                     /locus_tag="IWM59_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980682.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IWM59_002705"
     CDS             1356..1541
                     /locus_tag="IWM59_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP9980683.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IWM59_002706"
     CDS             1608..2003
                     /locus_tag="IWM59_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9980684.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IWM59_002707"
     CDS             1993..2370
                     /locus_tag="IWM59_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980685.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IWM59_002708"
     CDS             2371..2766
                     /locus_tag="IWM59_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980686.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DACDYM010000153         2963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDYM010000153 DACDYM010000000
VERSION     DACDYM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.161"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            747..2624
                     /gene="ltrA"
                     /locus_tag="IWM59_002709"
     CDS             747..2624
                     /gene="ltrA"
                     /locus_tag="IWM59_002709"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9980687.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            2755..2963
                     /locus_tag="IWM59_002710"
                     /pseudo
     CDS             2755..2963
                     /locus_tag="IWM59_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
BASE COUNT         1124 a          465 c          643 g          731 t
ORIGIN      
        1 agtgccataa tggtgcgacc tgttctgcag tgaaaagctg tgggttgcaa gtagtctaaa
       61 aactattgta ataactctta taacgagaag tcaaatgagt gggtaacgcc acaaagggct
      121 tccactgata ctccgacatg caacaagtag tttatgcaga ccaacgttgt gtgaagctcg
      181 gtgaagtcgg ctgaaggata ccgtaaggta aaagaaatag tgaataccac gaaagttctc
      241 tagaggtaaa gggtgtatcc gataagtcgg gtgtccaaaa aaacttaata tggtgagtat
      301 gtacaaacgt actgacgaac tagcgaatgt actgtccgaa aggcatagct cctagaaatg
      361 ggagagacag tgagcaaaaa atcactcatt gtgctaactt gtggttgagt aaaagtcgtt
      421 gttatgaaag accatgctgc gttacaggcg taggagaagt tagggggcaa aagctagcat
      481 ctaagttaag gcatgtggat agatataagg gaacagggga agccatcaac atggagagtg
      541 catactcgat gaagtggtag taagaaatat gtgaatgaca aataacttac tttagtgatg
      601 gtgaaagtag agtcacgact gatgaaggtt ctgtaatgga actggaggaa caggctcgat
      661 tcgtctgtaa aagtcatgaa acccgttcaa ccatgggatt ctcgtatgac taaaaaggtc
      721 acgagccaat tggagcgtga taaaccatga caaaagaaac aagtccaaaa gataggaagt
      781 tgcggcatgc agaatattat ggcatgacgg aaatcttcga taagatatac tccaaaagtt
      841 tgaagggaga taatttcaag aatctaatgc agattatcat ttctgataac aacattgtcc
      901 tagcatatcg taatatcaag agaaatcacg gtagtgtgac aagaggagaa gatggagtaa
      961 cgattaaaga tattgaaaat ctcaaccgta atgaattcgt agaaattgtt aggaagcgat
     1021 tcgcacatta taaccctcgg aaagtaaggc gtgtagaaat tcctaaatat aatggtaaat
     1081 taagaccgtt gggtattcca tcaatatggg atagaatcgc gcagcaatgc atcttacaag
     1141 tattagaacc aatatgtgaa gcaaaattca ataatcacag ctatggtttc cgcccaaata
     1201 gatcagcaga acacgcaata gcagattgta tggcccgaat gaatcaaagt cacatggaat
     1261 atgtggtaga tgtagatatc aaaggtttct tcgacgaagt caatcatact aaactcatga
     1321 gacaaatctg gtctatgggt atacgagaca aacagcttct cttaatcatt aggaaaatgt
     1381 taagagcacc cgtagtactt ccaaatggga agttgatata cccaacaaaa ggaacaccac
     1441 aaggcggaat acttagccca ttacttgcga acatcaatct caacgaattt gactggtgga
     1501 tagcaaatca gtgggaggaa aaagatgcca aagagctaaa acatcatttt aggaaaaatg
     1561 gagcttggga taaaggtaat atctatcaca agctcagaaa gagtacaact ctaaaagaaa
     1621 tatacctagt gagatacgca gatgatttta aaattttctg tagaaattat aatgaagcga
     1681 aaaaagtttt ctatgcaact aaaatgtggt tacaggagcg tctaaagtta ccaatatctg
     1741 aagaaaagtc aaaaatcaca aatctttcaa aagaagaaag tgaattttta ggctttacga
     1801 tgaaattagt gaccaaaggt aacaaaaagg ttgttaatac gcatataggt acaaaagcac
     1861 aaagcatgat taaagtgaaa ctaaaaaaac aaatcaaacg aattcaaaaa agtcctaata
     1921 gcaaagatac ctttatctat attggagtct ataacagtat ggttattggg attcataatt
     1981 acttcaaaat tgcgactcat tgtagtgtag caatgcgaaa aattgcaaga gaactcagac
     2041 caagttttga aatacgacta cgagaacaag gaatctcaaa agaaggacaa tacggaaagt
     2101 acgaaggata caagccttac ctacggtcag gacaaatacg atatataaga gggtttccaa
     2161 tattaccagt agggtatatt caacacagac atccgatgtg taaaaaaaga aatattaata
     2221 aatacaccga agaaggtagg gaagccattc ataagaagca acaaaccgta gaagaatgga
     2281 agatatcgtg gttgtgtagc aatccagtgc ttagtaagag agcaacgatt gaatttgcgg
     2341 acaatcgaat ttcaaaattc atcgctcaaa agggaagatg tgcagtaaca ggtgaggagc
     2401 ttatcttatc tgaaatgcat tgtcatcata ttatccctta tcatgaaagc aaaagtgata
     2461 gctatgaaaa cctagtgatt gttactgagg aggttcatcg ggtaatacac gctacacaaa
     2521 gtgaaacgat tgaggaactt ctaaaatatc taaaattaaa tccaaaacaa aaagaaaaac
     2581 tcaatgagtt gagattaaaa gttggtaacg aagaaatatc ttaataaata tttgtaggac
     2641 gggaaaacag aatgtctttt atagacgagg gaacgccgta tgcggtgaaa gtcgcacgta
     2701 cggtgtggac tgggggaaaa gacggagata atatcaaact cttacctatc agtatcgatt
     2761 aatccagatt ttattgaaac ggtaaattat gtcacaaact ttcaacgaga aatgcgtaaa
     2821 gtagaagcaa tgactgtttt agctacacaa tccccacaag aaatggtgcc agaaaatatg
     2881 tcttctgata acttctcgaa actgaagaaa gtctttgaat tttcttctac gaagatcttt
     2941 atgcgtatgg atgaatctat ttt
//
LOCUS       DACDYM010000154         2668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDYM010000154 DACDYM010000000
VERSION     DACDYM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.164"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            638..1063
                     /locus_tag="IWM59_002711"
     CDS             638..1063
                     /locus_tag="IWM59_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980688.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2215..>2668
                     /locus_tag="IWM59_002712"
     CDS             2215..>2668
                     /locus_tag="IWM59_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9980689.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLL"
BASE COUNT          868 a          439 c          474 g          887 t
ORIGIN      
        1 aaataaaaaa attcctcgat ttctttttag ggatcggtct tccagcaaga tttgcaagtt
       61 ggtaccaact tgcgaaaaaa aaaagaaaaa tccgcttctg cgaattcttc ttttttcatt
      121 ttggggcgct gccctaatcc ctaagtcccg gttattcaat agcatggaaa ctcaaagaat
      181 aagacgttcc ctggaataaa aaaaaggctg ctcgttcgag cagtccatac ataggaaaat
      241 gaaaaaacga cgtcccttac tgagaaacgc tattaaactt ttaacaaatc ctctaaaaaa
      301 taacaagtct ttaaaaagtg accggtaaaa atgcacaaag aactaaaaaa gagaaattgc
      361 atcaaatcaa gcattagaag ctagtaacta gctagaccaa ttgaaaatca caaagtgagc
      421 gcctgtatct attgctacag gcgctacttg gcatagtgta cattatgtca acaagaattt
      481 aatataacat atttttctag tttgttataa caatgccact ctaacaaaaa tgtaagcatt
      541 atccttatta aaagctcctt gacaaggact atacctttta ttttatgatt tttacataga
      601 aaacgttttc tctatttttt gtttagaggt gatttttatg atttgcaaga acaagaaaaa
      661 aattattgct attattgcta gtttctcttt gatttttagt actgcatgta ctgtcttatt
      721 aacaaataat gctaatgttg aagctgcaag ctcttcttac aaacataatc atgctggtta
      781 tagatgttac aaaatgagtt attatgtaag tccttctaaa tgcaaacaat tggctcgcca
      841 aactaataaa cttaataccg tctcagctat tgcaaacttt attggactat ctggattaac
      901 accggggatt atatcattag ttttcggatc agcagttagt gctaataatg tatttgttac
      961 agccgctaga agaggaaaag gcgttcaatt aaattatatt gcccatttta gtaataccac
     1021 tacctataat tatagttcta accaaaatta tgttataaaa taattaaaac tttaaactag
     1081 gaggaatgct ttatgaaatt aacaaaaatt gctgtaattt tagctgttat tgctggcatt
     1141 attttatatt ttgtattgcc tgctaatcaa ttcttccttt actttggaat acctgcgatt
     1201 ttaatagtaa atggcgtaag aatctttgaa caacgaaaaa aatgtagctc tacctaatcg
     1261 ttacaaaaca acaatttcac cttcggtgaa ataagcgaaa ctttacaaaa agtggctagc
     1321 aaatgctagc cttttttgta ttgtagagcg tgaattttga gggggattca agggggagct
     1381 tgcgcccctt tgacaagcgg ttatttgcac caaatacctc cgtatttggc tagcaatcct
     1441 tctaaaaaat acacctatcg atcagagtca aaacaaatca attttcgagt gagcgaatcc
     1501 gaatttgcaa agctgaagct ttgcaaatga gtgtgccggc ttccgtaaaa atacatatgg
     1561 ctataatttg cgttttaagg cgttttaaac gttctggtat ataattatac caaaacaact
     1621 ttaaaacgcc ttagatctaa ttttagaggt cttatagaac attttagggt tgttagggct
     1681 tgccctgacc gcctgtaagg cgctttgaga acaagataag ggttttatac taagactgtc
     1741 ttaaaacggc ttaaacaaac gctcagaact tttcaaagga gttctttatg aactgttttt
     1801 aagcaaagga gcctttaggc gactgcgcag tataatgtga gagcgtagcg atcacacatt
     1861 tttagtgacc gtagggagcg agtttcccag tttagaaaaa ttcgcgaact tgtttgagga
     1921 aaaaagcagg agttttttag gcgggaaatt ttgcctcctc ttttataatc tctttttaaa
     1981 cctcttttta aacctcttta ccgtcggctg tatgcttact ctcccaaggt gtgcagaatc
     2041 ttttgggggt ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt
     2101 gtctgttttt gggggtcttt ttgtctgttt ttaggggtct ttaattaggg gcttgacccc
     2161 tattagaaag ctctttataa ttttgttaag tagttaatct tgaaggagga ttttatggca
     2221 aatgaaattg tgaaatacca ccatgaatta aatacgattc ctttacgcaa atttacgtca
     2281 gtagaaatga atttattctt ctctatagta tctagaatgc gtgacgtagg cgataaaaaa
     2341 gtacaattta cattcgaaca attaaaagat ttaagcaatt ataaagcaac tgcaaatgtt
     2401 cgattcatag atgatttaga aacaacgtat gacaaattaa tggatttacg ttttggtaga
     2461 agaagtgctg acggtttgca acgtgaacga tttgttttat ttaatcaatt taaaatcgat
     2521 ggaaaagctg atattccttt tgctgaaata caagtccatg agaaagcctt acccttactt
     2581 aataatcttg aagaatgggt tcgatattct ctccaacaat tcaatgaatt agaaagcagt
     2641 tattccaaaa ccatgtttag attattaa
//
LOCUS       DACDYM010000155         2533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDYM010000155 DACDYM010000000
VERSION     DACDYM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.165"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            278..541
                     /locus_tag="IWM59_002713"
     CDS             278..541
                     /locus_tag="IWM59_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP9980690.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            535..885
                     /locus_tag="IWM59_002714"
     CDS             535..885
                     /locus_tag="IWM59_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9980691.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(909..1523)
                     /locus_tag="IWM59_002715"
     CDS             complement(909..1523)
                     /locus_tag="IWM59_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9980692.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1837..2259
                     /locus_tag="IWM59_002716"
     CDS             1837..2259
                     /locus_tag="IWM59_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980693.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2269..2472
                     /locus_tag="IWM59_002717"
     CDS             2269..2472
                     /locus_tag="IWM59_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980694.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
BASE COUNT          892 a          370 c          428 g          843 t
ORIGIN      
        1 tttctatgtc ctttttattc cattttgtat caataactat gaaatcttta aagtatccag
       61 aagagattat ttttagatca atttttttct tacctgattc taaagatttt gttttcttaa
      121 acatattatg atttcctttc gactattttc atataaatgt taacatagtt tatggattca
      181 tggaatgtta atctaattta atagaaaagg ataattaagt atatacgttt tttcttatgt
      241 atgttatact atgtatatac agaaagaggt gtctactatg gaaatgttac ctataaaaaa
      301 atggggaaat agtaatggcc ttagattgcc aaagtatatt atggaatatc ttggaattca
      361 tacagaagac aaagtgaaga taatacaaga agaagtaaac ggtcagaagc ggctaattat
      421 cgaagcggct aatatagaaa atgaattaac aattgaacag ttatttgaaa attacactga
      481 tgaaagaaat catgtgacga ttcaaaattt aggtgaagca gtaggtaatg aaaaatggtg
      541 agaaaaccta gacaaggcga tattctatta ttgaatacag cacctcgatc tggacacgaa
      601 cagacaggta aaagacctta tattgtttta agtcatgata ttattgcgga ttatagtaac
      661 gttgttacag ttgctccaat ttctagtaca actagaaact atccgctata tgtaaatata
      721 aactcagaat atgaaatgaa gacaacagga aaagtattat tagatcaact aacgacaatt
      781 gattatgaag ccagagagtg tatttttttg gaaaaagcgc atgatagttt agttgaggag
      841 ttattgatga aagttagaac agtttttcaa aaagtaagca aataaaatag aaacagcagc
      901 atgatcaact attcgtgatt gaccatgctg tcttttttta ttctatatac ggtttgtctc
      961 gtaacattgt agtcttttgc aatcttagcg atcgcaacac ctttttttag atcctcaacg
     1021 atgtttttat aaacaagacg acgttgggga tcttttgcat tggcactgta tagcttagga
     1081 cgtcctttat aaacaccgtt cgcttttgca atcttaatgc cttgtgcctg acgtcgttta
     1141 gattcggtac gttcttgttc agcgatcatg gccaaaattt gaatgatcaa atctttaatg
     1201 aatttatcca ggagaggatt gccaattgct tcggccatga tcggtagact tgtgatgatc
     1261 agctgcacat ttttcttctt caagtaattg actgaagcga tgatctcgtc gtaatttctt
     1321 ccaagtcgat caatagcttc cacaataaaa atatcttgat ctcggacgaa atccagtgct
     1381 tcttgaaaaa caggtcgatg agtaatcgtt gcgccagatt gtttctcaat gaaaattctc
     1441 tctgcaccga attttttcat ttcttcaatc tgccgttgtt cattttgatc gatggatgag
     1501 acacgtatgt atgcaactct caaaattttt acctactttc tgcaactatt taaaaaacac
     1561 cctaatcata cacgagaaaa ccagattttt caatgtcgcg gtcgtgtcta gttagggtat
     1621 accttatata gacaaaaact aatgatggag gaaatagtat gtgtcacttt tctttttaaa
     1681 tggcaatatt ttttgtaaat aaatgtcaat caagaaaatt taaacgatta tgacattaaa
     1741 aatatatttt atgaattttc tagtatataa cttgatttat ctgttaagat ttattaagaa
     1801 aacgttttct tatttttgat ttcgaggtga tagacaatga aaaataagtt tattttatct
     1861 ttaggtattt taactattgg aattagtgta actggaacta caattcctac atttgctgat
     1921 agtacaaatg aaccaactac tgtagtagat aataaccaag aattaactcc tttagatagg
     1981 aacggaatag ccattggtaa cccaactaac gacttaactg ttactgaaaa ttatttaata
     2041 gtttctccat tacttgctcg agcagcttct aaaacgatac gagtgacagg aacgatgatt
     2101 tttgataaag ttggttggcc ttctagacct cctcaatcac ggaattggaa agaaaaaaga
     2161 aatggttatt ggtactatgg aaaattgtat ttacaaagtt ataaatcagc tggaaacaat
     2221 tggattgcga cttattcagg aaccttaact agaaagtagg gattttttat gccagaattt
     2281 gtgaacacac caaattttta tcaagatagc gatattctaa gtaaaattaa tttaccgaaa
     2341 gaagaatcag ctttacaagt tcagcgaact ttacaggaga cacaaaaaat tcaaaaatca
     2401 atttctaatc tagaagattc tgaagaaaaa agaaaaaaaa gagaccaaaa aacatatcaa
     2461 aaagaaaaat aatgatttta aaaaaagagt gtctagtcga cattcttttt ttctaattga
     2521 aagtgttcat aaa
//
LOCUS       DACDYM010000156         2520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDYM010000156 DACDYM010000000
VERSION     DACDYM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.166"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..537
                     /locus_tag="IWM59_002718"
     CDS             <1..537
                     /locus_tag="IWM59_002718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980695.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            564..2375
                     /locus_tag="IWM59_002719"
     CDS             564..2375
                     /locus_tag="IWM59_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9980696.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         1007 a          395 c          555 g          563 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatactcat tctcctgaag
     1141 acgtcaaaga acttttgaac tttatgtctc gttttccaga acgatccttt agaaatcaat
     1201 tattgattga ggaacaattt ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa
     1261 aagaaggaat ctatatcaaa aaaggggaac aagggaacaa gatctttgtc agaaaaacgg
     1321 tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga aatagaagct acaccagaag
     1381 ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa aaaaacttac tacacgattg
     1441 aaaaagtgtt tgatatcaca caaacacagt taaaacctga agactatccg aaaatctttc
     1501 cgaaccgtgt ctttgatttt caattggata aaaaaggaca gtcagaactt caagctggga
     1561 ttcaagcggt agccaacaat attggaatac aaattcgaga tatgacagaa agtgaagtct
     1621 atcaaagaga attaggacaa gcacgtgggg cgtatgttcg taacgaattt actggcgaag
     1681 aagaaatcgt catgaatact cgaaatactc ctacgcagca tttagctaca agtattcatg
     1741 aattagccca tgcaagaatg cacaaatttt cagaattaga taccgcaact aaagaattgc
     1801 aggcagaaat gacttcttat attgtgtgca aacattttgg aatggacacg ttggagaaag
     1861 ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat aaggaagctg
     1921 cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc attcaaacca
     1981 tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca gcagaagtga
     2041 agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtt cctgttggtt
     2101 cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt cgaacgttac
     2161 atttgaagga tgcggcgttt attgaagatc cacagcataa tcaagtaatt ttaaaaggaa
     2221 aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg ctaggaaatt
     2281 cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat gaaatctatt
     2341 atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga ctatctgaaa
     2401 aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa atgagtctta
     2461 ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga atggctgtag
//
LOCUS       DACDYM010000157         2478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDYM010000157 DACDYM010000000
VERSION     DACDYM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.167"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            195..641
                     /locus_tag="IWM59_002720"
     CDS             195..641
                     /locus_tag="IWM59_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9980697.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            914..2266
                     /locus_tag="IWM59_002721"
     CDS             914..2266
                     /locus_tag="IWM59_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9980698.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT          884 a          391 c          493 g          710 t
ORIGIN      
        1 ttaaagatga aaagaacaca caaaaattta tgatgagaaa aattatttga atagagacgt
       61 agaatagtga acgcgactgt tttacgtctt ttttcattaa aatacaataa aattgaattt
      121 tcttttcatg ttaagtggat tttaaaatgt aaaaatagta tgataagacg agaataacaa
      181 cgtggggtgt ttacatggaa aataagttag tcgaagaggt tatcgaaaag ttaaaaaaag
      241 agaacattcg aatcacacct caacggtacg cggttttaga atatttgatt gaacatcatt
      301 cccatccaac ggctgatgag atttataaag cacttgaaca tcggtttcca aatatgagtg
      361 ttgcgactgt ttacaataac cttcgcttat ttacttcgat tggatttgtt caagaaatga
      421 agtacggcga tgcttctagc cgatttgatt tcagttcaaa gcgtcattat catgtaattt
      481 gtgaaaactg cggtaagatt gttgattttc attacccagg gcttgaagat atagaaatgg
      541 ctgcaagcag actgactgat tttgagatta atgaacatcg gttggaattg tatggactct
      601 gtccggattg taaagagtca gcaaaaaagg cgcaaatgta aaaacagttt ctacaagtgt
      661 tatacctaaa aaaagctagc tgtgtaaaaa aaacgctagc ttttttattt ttttgtcttt
      721 tattcacaat cttagatata aaagtgaata ttattaaaat aagaatcttg tagatattgg
      781 gttcttatgc ttgaaaagga atgaaactac aaacaaacct ttgtgaattc acaagcatta
      841 aactgttgta tccgttttat ttctgttata gtagttttgt cgaaggacaa aatgttaatc
      901 ggagggacca taaatgaaag tagtagtaat tggctgtact catgcaggta ctgcagctgt
      961 aaaaagtatt ttaaaaaatc atccagaagc agaagtgaca gtatatgaaa gaaatgacaa
     1021 tatttctttc ttatcttgcg gtatcgcact ttatgtcggc ggagtagtaa aggacccagc
     1081 aggcttgttc tactcaaatc ctgaagaatt atcttcatta ggagcaacag ttaaaatgga
     1141 acacgatgta acagacgtcg atacagaaaa taaaacagta acggcaaaag atctgaaaac
     1201 aggagaaaca gaaacggttt cttatgataa attagtgatg acaactggtt catggcctat
     1261 tatcccaccg attagtggaa tcgatgcgaa aaatgtactc ctatgcaaaa actacaatca
     1321 agcaaatgaa attattgcac aagcgaaaga agcaaaacgt gtagtaattg ttggtggtgg
     1381 atatatcggt attgaattag tagaagcttt tgtcgaatct ggaaaacaag tgacattgat
     1441 tgatggatta gatcgcatct tgaataaata tctagataaa ccattcacag atatcttaga
     1501 aaaagaattg acagaccgcg gtgttactct tgcgctaggc gaaaatgtta ctgaatttat
     1561 cacagatgaa gaaggaaaag tgaaacaagt agctacacca acagatacat ttgatacaga
     1621 tatggtcatt ttatgtgtgg gcttccgtcc aaatacaaaa ctgatcgaag gaaaagtaga
     1681 tacactgcca aacggggcaa tcaaagtgaa tgaatacatg cagacaagta atccagacat
     1741 ttttgcagct ggagactctg cagtagtcaa ctacaatcca agtggcacgc aaaactatat
     1801 tcctttggca acaaatgccg ttcgtcaagg tttgcttgtg ggaaataact tgacagatca
     1861 taaaatggcg tatcgtggta ctcaaggaac ttcaggactt tacctatttg gctggacgat
     1921 cggttcgaca ggtgtgacca aagaaagcgc aactttgaac gatctagaag tccaagcgac
     1981 tgtatttgaa gataattatc gtccagagtt catgccaact actgaaaaag taatgatgga
     2041 gctagtctat gaaaaaggaa cgaatcgtat cgtaggtgct cagttcatgt ccaaatacga
     2101 cattacacaa tcagcaaata ccatgtcttt agctgttcaa aataaaatga ctgtagaaga
     2161 tctcgcactt tcagatttct tcttccaacc acattttgac cgcccatgga attacttgaa
     2221 cctacttgca caagcagcgc taggcgaaat cgaaactgca caataaagaa aaagggttgt
     2281 gacacaattt tgtcacgacc ctctgtcttt ttttgtgaaa agtacaatta gttgtctttc
     2341 ttcgaatttt ttgtcacaat aagcagcaaa gataaagctg ttacataaaa gaacaagcag
     2401 ctttatttga ttttgaaaag aaaggtgaga aagaagatga ataaagaata ttttgtccaa
     2461 caagatgcat ttttagaa
//
LOCUS       DACDYM010000158         2420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDYM010000158 DACDYM010000000
VERSION     DACDYM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.168"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..288
                     /locus_tag="IWM59_002722"
     CDS             <1..288
                     /locus_tag="IWM59_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980699.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(801..874)
                     /locus_tag="IWM59_002723"
     tRNA            complement(801..874)
                     /locus_tag="IWM59_002723"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(838..840),aa:Ile,seq:cat)
     gene            complement(1063..2082)
                     /locus_tag="IWM59_002724"
     CDS             complement(1063..2082)
                     /locus_tag="IWM59_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9980700.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2093..2299)
                     /locus_tag="IWM59_002725"
     CDS             complement(2093..2299)
                     /locus_tag="IWM59_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9980701.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          739 a          466 c          405 g          810 t
ORIGIN      
        1 gagacacaat tatggtttaa atttgaccct tcgaatagat ttatcaaaga tttttataaa
       61 gtatggaatt cagaagtttt ctttttagca atcgaagata gcttattaat caatctctac
      121 tattctaata agaactattt caaaattcct gctgcgaaaa ctagaatgaa gaaggatgta
      181 tactttttgt ttgatatcgt gactgacgtg ccggacgcac gtagcgatca tcggcgttat
      241 gactatataa agtatacttt cgttgatcca gaaagataca aagattaaag taggctacct
      301 aaaaaggtag cccggaacga attttatcac catacttgtg aaaggagata ttttttaagt
      361 tagtattaag attgtgtaat atgatgatat ctatatttta tagtatcagt gctataaaat
      421 caaaaataag tcactaatta actaccactc caattgtaag ccttttttct cacttttttt
      481 caaaaatatg gtatgccttt taatagcttc aaatataaaa gagtttaaag cgtaacacac
      541 ttatggggaa gtggcttggg gtgcgcttta aactcttctt tattatcatc tcacaattta
      601 acccaaatgt ctttctattt aaaaatcaaa gtaaaacttt tcaaatatac agaaatataa
      661 ctatgtgaaa catcctttca ttaatccata aaaggataca taaaaaagcc actcatttga
      721 gtggcaatga agaaaagctt tagcttgtat aatactcttc aaaaaattct aacacagaac
      781 gattcaaatg gctacgttaa tgtaccctgt aggactcgaa cctacgaccg gacggttatg
      841 agccgtctgc tctgaccaac tgagctaagg gtactggttg ttgccacata aagccataaa
      901 caatcaacca gtagaatgtg tggcaacaaa cctgttatcg catagcttgg agtgtgacta
      961 ttatgggtga tagtgaagat atgcgataac atcactattt tatcgaatga tttttatagt
     1021 tgtcaatata gttatgtaat gctcctcaac gaggagctat ttttatcgtt taggaatatt
     1081 taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc
     1141 attcgttgcc cgcatcatga catagacttt cttattaggg aagttacgca tgttaaaaga
     1201 tacatgatag ccaacatttc cagaagtatt ataagcttga tttacatctg atctataaat
     1261 tccatcagct cttactcgag ctaattcttt cccagtattg taatccataa tgaagatata
     1321 ctcgtattta tagttagcaa tgtgccatcc agctacatgc aaatttgcat tttcgatttc
     1381 tccaaactga tcaatgtggg cgtaattcgt tccatctgtc agtgtaggat tagctgcacc
     1441 agctcgtgtt ggatcaatga caggcttgtt ttcagaagtt gttggatttt catcggtaaa
     1501 tccatgagct aaatcatatg ctaatttttc tttacttacg cccatttcag aaagataacc
     1561 gtaaggatct gtatgatcgc cccagatgtt ttgtgttacc cataaatgcg atttgattcc
     1621 cggttggtta taaggagtgt ccaacgttaa tggaatacca tatttcattg ctgaatctct
     1681 agccaattca acgtatgcct tgtagttttt ctcaaacgtt gctttatcat gtgtgtgttg
     1741 taactcaatc tgcacaggac tgttggcatt agcatacgaa ccagcaccgt actgtacata
     1801 accaggttga ccgacttgat aaacaattcc gccgtctccc acaatataag cagtataagc
     1861 gctagtccat gaacgttgca tatactgcgc ttcattgcgt cctgttgctg tttcattagc
     1921 cgtttcatgc agtaaaatat actgattatt tgctacttgt gagctacctt catttgcgcc
     1981 caaattaaat tcattgttga tagtataggc aaatccgtta attggcaata aaaaaagagc
     2041 cgttaacagg ctcatcgcag taatagtaat tttctttttc atatctatcc tcctatttat
     2101 ctgatgaaat tttcttatac gttcgatttg atacacctaa aatactccct agaaatgctc
     2161 caaaacctgt aataatcatc accgttatat ctgtgtaccg ccagttaaca ccttttccga
     2221 caagaccgac aaaagtcgct aaagcgggga taactactat agcgatccat ttcaaaatat
     2281 caaaggttct attgttcata ctatccacct cccttcattg gttgggctac tttctttttc
     2341 aatcgcttgt tttcttctat caatatttct ctttctgaaa gcaaggcatc aatttgcccg
     2401 tccagttgtt caattttatc
//
LOCUS       DACDYM010000159         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDYM010000159 DACDYM010000000
VERSION     DACDYM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.169"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWM59_002726"
     CDS             207..2069
                     /locus_tag="IWM59_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9980702.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IWM59_002727"
     CDS             2069..>2398
                     /locus_tag="IWM59_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9980703.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACDYM010000160         2396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDYM010000160 DACDYM010000000
VERSION     DACDYM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.170"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..93)
                     /locus_tag="IWM59_002728"
     CDS             complement(<1..93)
                     /locus_tag="IWM59_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9980704.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREV"
     gene            complement(167..448)
                     /locus_tag="IWM59_002729"
                     /pseudo
     CDS             complement(167..448)
                     /locus_tag="IWM59_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2292
                     /locus_tag="IWM59_002730"
                     /pseudo
     CDS             910..2292
                     /locus_tag="IWM59_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          872 a          409 c          399 g          716 t
ORIGIN      
        1 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
       61 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      121 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa tgagtatagt
      181 atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa ttgatatctc
      241 gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat ggctcagaaa
      301 gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat atttcgattt
      361 ttctttcata ataagatctg ttttggtcct caaatttagc caataactca aattgattta
      421 ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt tcattaacaa
      481 aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg tgaacacgaa
      541 ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc atacgttctg
      601 gactatttgc taatagatta aaatttcagc atctatttct gaccataaat aaatgagctg
      661 ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa gtttctgacc
      721 ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa aagagtattc
      781 aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag aaccagaata
      841 agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa acttaggagg
      901 aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt tttaaaatac
      961 gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg tgcactacgg
     1021 aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg aaaaaaatca
     1081 aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata tggtcaatgt
     1141 tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg gcagcggtgt
     1201 gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt aacacaagat
     1261 caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa aaatttagcc
     1321 gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt tcttggaaag
     1381 gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct acttttaaag
     1441 gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc attactcgaa
     1501 ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct atcaaatcaa
     1561 gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga gcttcttcaa
     1621 cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga gaaaccagat
     1681 aagtattaca aagaacgtca aattaattat cacgattttc aatcataact tctgtgcgta
     1741 tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt ctgaaaaagt
     1801 tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac gaaaaaagta
     1861 ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc ggaattttaa
     1921 agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa ttctcgcagg
     1981 gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta gttggactat
     2041 tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc agataattca
     2101 gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg tgactggaag
     2161 aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg gtataataat
     2221 caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa gaagcaaaga
     2281 tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc cttttttttg taaaaaacta
     2341 ttgcacaata caagtagcct acggctatcg aaatttccat aactttcgag caagaa
//
LOCUS       DACDYM010000161         2395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDYM010000161 DACDYM010000000
VERSION     DACDYM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.171"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            316..570
                     /locus_tag="IWM59_002731"
     CDS             316..570
                     /locus_tag="IWM59_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9980705.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IWM59_002732"
     CDS             571..888
                     /locus_tag="IWM59_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9980706.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="IWM59_002733"
     CDS             1447..2382
                     /locus_tag="IWM59_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980707.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DACDYM010000162         2096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDYM010000162 DACDYM010000000
VERSION     DACDYM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.172"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            149..667
                     /locus_tag="IWM59_002734"
     CDS             149..667
                     /locus_tag="IWM59_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP9980708.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            674..1186
                     /locus_tag="IWM59_002735"
     CDS             674..1186
                     /locus_tag="IWM59_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980709.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1457..2080
                     /locus_tag="IWM59_002736"
     CDS             1457..2080
                     /locus_tag="IWM59_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9980710.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          823 a          254 c          341 g          678 t
ORIGIN      
        1 tttggcgagg tagacgagct ctaaagcttt atttacatgc catccgcttt gaacggacta
       61 cactgggcat gtgacagcat gccctttcat ttatattata tctcattctt ttatataatg
      121 aaagtaaata aatcatggag gataaaatat ggataaattt aatagattaa cattaataaa
      181 tcagtttgaa attttaaaag ctttaaatcc taacgaagaa aaatactatg ctgaaaaaat
      241 agaaatacta acagaaggtt acgaatatca ctatagtgaa atatttgaaa atatttctga
      301 tccattgcca gaagaagatt cgcggtttgt tctagacata ttaaatatgt atagagatat
      361 tacttttagt aaaaataaat taaaagatat aaattcaatt gataattatt atatacaatt
      421 taaaggtttt gactttaata gctctaccga atttaagtta gcactttatg cacaattttt
      481 tattgaaaaa ttaaataggt ttcaagaaat agttgaagat agtgatttta acacttttaa
      541 tagccataaa ccgatgagag gtacttatat aaaatttctc gaaaattata atgatttaaa
      601 atctaccaat aattatcaat ttggaaactt gtcttatgag atgataagta aagttctaag
      661 tttgtgatga taaatgaaat taaatagtat aaatgattta tttgctatta taggtgttat
      721 tgctaacata cttttttcgt gtttgaattt tagatttact caaaagaaca aaaagaacaa
      781 actcagtata gaacaaatga aaaaagacat tgaaactgat ttaaaaaatg accataaaga
      841 aagaagaaaa attgaagaat atacttcttt aatggaaaag tgtaaagcaa ctgatgcaat
      901 ttttagcttt ttaagaaaaa gtgatgagat aatcacacgt gaaaatatag atttagaaaa
      961 tgagattagt aaacttgaat ctatcttttc cgatataaat tctcttaagt atttaatcgt
     1021 cgaagaaaat tcggatagct ttttagaatt tgaaaaatat ctaacagatc ttatagataa
     1081 tagaaataat gaattaatga gacaaaacat tgcaagattc cttactaaat tagtttctga
     1141 atggaaaagt attatttctc aaaatatgga taaattaaga tattaaggat gttagtttta
     1201 aaataaacta atgtccttat ttttgtgttc acatttctgt tacctaagaa atacttaaag
     1261 gaggtgatgt agccagcaac gactacaact tgaacccgct atctacagtt tatttgttgt
     1321 ttcttttggg tatactctaa tggtacaaat ccatatttgc ttaaagcgtt gatttttcag
     1381 cggtttttct ttattgcaaa gtgtacccta agacctgtat actaatagta aatagattgg
     1441 ataggaatga tggcaaatgt atttagctta tttaagagtt tcttccgacg atcaaagttt
     1501 agctcggcaa cataagctaa ttgaaaattg gtcagtcaaa aataatattg ctgaagacga
     1561 tttaaaaatt ttcgaggaaa aagtctctgg aaaaaatatt gaaaacagac cagagctgca
     1621 agagcttatt tctttcatac gtgaacgaga tacgattatt attcctagtc ttgatcgatt
     1681 agcaagaaat agtaaagata caaaagactt acttcaattg tttcgtagta aaggtgcttc
     1741 tattgaaata ttagacctac cgtcgtttaa tggggtaact gaccctgcat taagagactt
     1801 gctaacaaat ttagtcatag aagtgtttag ctatgttgct gaaaacgaac ggaaaaaaat
     1861 caaagagcgt caaaaacaag gcattgaaat tgcaaagaaa aatggtcgtt atactggtaa
     1921 aaagattcaa taccatgcca atgcaacagg aaaaaacaaa ttgatttacg atgaaattgt
     1981 tgatggtctg aagaaaaaag aaactatttc taaaatagca aagaacgcag gagttacaag
     2041 aaaaaccgtt tatcgagtaa aaagagattt aggaatgtaa tatgttccag ttagaa
//
LOCUS       DACDYM010000163         2082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDYM010000163 DACDYM010000000
VERSION     DACDYM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.173"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..971)
                     /gene="cysK"
                     /locus_tag="IWM59_002737"
     CDS             complement(45..971)
                     /gene="cysK"
                     /locus_tag="IWM59_002737"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9980711.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1111..1998)
                     /locus_tag="IWM59_002738"
     CDS             complement(1111..1998)
                     /locus_tag="IWM59_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9980712.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          578 a          468 c          353 g          683 t
ORIGIN      
        1 taaaagtttt ttacataaac ctatctggag aaaaaccaag tgccttatat ctcttggtaa
       61 agggctgtag ataaataacg ttcaccattg tcgggaacca cagctaatac cttcttgcct
      121 tttcctaatt cttttgcgac ttttagcgcg gcagcaattg cagcgccaga tgagatgcct
      181 actaaaatcc cttcttttcg cccaacttct ctagctgttt ccatcgcttc gtctccacta
      241 atagaaagga ctttatcata cacagaagta tccagtgttt taggaacaaa acctgtaccg
      301 attccttgga ttttgtgagg tcccgcttct ttcccttcta aaatagcaga ttctgctggt
      361 tctaccccga ttaattcgat tttcggatac acacgtttta attctcggcc agcgccagtg
      421 atcgttcctc ctgtaccgat acctgcaaca aacgcgtcca agccatttac tccaaatgct
      481 tgatgtatct caggaccagt cgtcttctca tgcacaagag gatttgcttc attttcaaat
      541 tgtaatggta aaaagtagcc gttttctttt gccaaacgtt ctgcttctct gattgatcca
      601 gaaataccat ccgcaccagg agtcaaaatc aactctgcgc cgtacccttt catcaataaa
      661 cgtctttcga tactcatcgt ctcaggcatc acgatgatta ctttatatcc ttttgcaact
      721 cctaccatcg acaatccgat tcctgtattt ccagaagtag gttcaatgat agtatcacct
      781 ggtttcagta gaccatcgtg ttctgctttt tctatcatac ttaaagcaat acgatctttt
      841 acacttcctc ctggattaaa aaactcaagc tttacaaaaa catctgctga atcttctggt
      901 acgattttat ttaatttgac gattggcgtt ttgccaatca attcggtgac tgagttatag
      961 atagctgtca tcttcaattc ctccttgtct ttgctcttct tacttatcat aatacaatct
     1021 gttcatttat ggcgagtgat tcgtctttga ctagcaaaaa aaagacaaag atataccata
     1081 tatggtaatt tctttgcctt tttatctaaa tcaaacgttt agttcttgac caactaataa
     1141 agaataactg gacatagaca tgccattttt cgccattaga tcatcaacag acatgccaaa
     1201 tttttgtgca atatcccata gcgtatcacc tgacacgact gtatatgtct gaccattatt
     1261 tgtatttaca tctgttgttg aagatgatga cgttgaagtc gtcgtagtag tcgttgttgt
     1321 tgtacttgtg gttgttgtag ccgaacctgt tgatggcgta tcaaaacggg ttaaattata
     1381 agtattaatc aatgagttca atttggcccc ataatttgga tcggtagcat aacgacctgt
     1441 caagtacgct gttgcatcat aaaaacttgt tgtattgctt ttccacacgc cagcataatg
     1501 ataatcgccc gtggaaaaac tggttgaacg aagaacattc gcatggtctt ggaaggattc
     1561 ccaataagaa gaatattttc ggaatggctc attcatcgtt acccattgtc cgtttacata
     1621 ttctttggtt gccatataaa cgacctgtcc gttatagctt cctttcacac cgaatagatt
     1681 ataatttggc gcactagcta atccggaagt gccatatgca ctttccaaca acgcttgagc
     1741 gatcatgact gaagcataca agtcattact tgcagcaact tctgacgcag accagccgat
     1801 ttgatcgata aacgcttgag gatcagcgac tgtttgagat ccttgttcat ctgcatgaac
     1861 atgttgcggt accgctgtaa ccatcatcgg cccaactaaa agactcgttc caattaaagg
     1921 agcaatcgct tttttattga tcaccatttt tcttttaggt ctatggtgcc gggcactgcg
     1981 ggtcttcagc tcttgcattc aaattcctcc tcaaaataaa aaaacttaat aaaaagttct
     2041 cttcttaaaa caaacttaat taattaaatt atacttttaa tt
//
LOCUS       DACDYM010000164         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDYM010000164 DACDYM010000000
VERSION     DACDYM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.175"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWM59_002739"
     CDS             <1..378
                     /locus_tag="IWM59_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9980713.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWM59_002740"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWM59_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9980714.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IWM59_002741"
     CDS             1859..>2003
                     /locus_tag="IWM59_002741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9980715.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACDYM010000165         1990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDYM010000165 DACDYM010000000
VERSION     DACDYM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.176"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWM59_002742"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWM59_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9980716.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWM59_002743"
     CDS             852..1040
                     /locus_tag="IWM59_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9980717.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWM59_002744"
     CDS             1053..1610
                     /locus_tag="IWM59_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9980718.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWM59_002745"
     CDS             1642..1884
                     /locus_tag="IWM59_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9980719.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          307 c          406 g          553 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc
//
LOCUS       DACDYM010000166         1952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDYM010000166 DACDYM010000000
VERSION     DACDYM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.177"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            155..1861
                     /locus_tag="IWM59_002746"
     CDS             155..1861
                     /locus_tag="IWM59_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9980720.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          697 a          387 c          353 g          515 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aa
//
LOCUS       DACDYM010000167         1895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDYM010000167 DACDYM010000000
VERSION     DACDYM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.178"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1028)
                     /locus_tag="IWM59_002747"
     CDS             complement(<1..1028)
                     /locus_tag="IWM59_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9980721.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1110..1799)
                     /locus_tag="IWM59_002748"
     CDS             complement(1110..1799)
                     /locus_tag="IWM59_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9980722.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNGL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
BASE COUNT          641 a          426 c          327 g          501 t
ORIGIN      
        1 acagtattat ttcggttgct tgctgcttca cctagccctt gtactcgtgt ttgattggcg
       61 acttgaccaa attgattccc gacgtctaat aaaataatag ctaaaagcaa aatggccaaa
      121 tggcttccgc caaccaataa agcaataaag cttattgttt gcataacaag tcccatatac
      181 acgatttttc tttcagaata agaatctgcc agtcgtccca cgataggtgc agcaactgct
      241 ccagacaaac caaaaatcgc taatatccct gcttcgaatg ttccccaatt gtaggcggga
      301 ctgctgatat aaaaaatcaa cgtagaccaa aagatcgaaa atgttccaaa catgaaaaat
      361 ccactgactg ccgcttctct taatagacgt tgagaagcaa ctaaatgcgg caaactttta
      421 agtgaatcaa ggtatgaaac agctgtacta ctttgctgga cagaacgcgg cattttccag
      481 taaagcagaa gtgtaagaat tccgactgca cataaagcaa ataaatagat catccgccaa
      541 gaaaaaagac tggcaacaat accagatacc gtccgagaaa gcaaaatccc cacgagtaaa
      601 ccgctaagca atgtacccat gaccttccct cttgcttttg gtcccgctag taccgcaccg
      661 taaggaatga tgatttgcgg cacgatcgaa agtaatccta ttaaaaaaga agcgcatgca
      721 aataacgtaa acgaaggcgc aaaaaaagcg gccaacagag agacggcaga taacgctacc
      781 attcgaatga tcaattttgg acgatccacc acgtccccta aaggaactaa aaacagtaac
      841 cctaacgcat agcctaattg ggtcaacatc gtcaatatcc cgactgcact tgtgctaaca
      901 gataaactag ctgctacttt tgttccaatc ggctggatgt aatacatatt ggcaacaaca
      961 acgccacaag tgattgataa tagtaaaata agcgaagttg ttaaaggtgt tttttcatta
     1021 ctcttcattg atttccccca tttcctatct tagtttacaa cttatggaaa agattggaaa
     1081 tagaaaaata caactattcg ccacacttat tatttgtgct tttgttttcc ttgttttttc
     1141 ttgcgatctt tataaatgaa gacaactaac cctactagca cagtaacact tgcaaccaac
     1201 gtgactgcag ttttgttata tccgtgaaac agagcttctc caccacacgc atacaacaca
     1261 gacgaaggga tgactccaat cacgatcgac aatatgattt gctgtagagg caatttcatc
     1321 gcatctgcag caaaattgac catagaagat ggaataaccg gtatcatata gcctaacatt
     1381 acacctattt ttggatgttt catttgtcgg attgcttgca cccaatgatt cgtttcggtt
     1441 ttcttatcta aaaattttaa gcgatgcata aggaaaattg cagtgatgtt accaagacca
     1501 ttacccaaaa tgttgatgat cgcacctgtc agtggtccaa agctcaaacc agcaatgact
     1561 ccaactactg aagtaggaat acctggaaca gcacacatca ccgctgtcaa tacgatcaag
     1621 agtatcgatg caaaagtccc atgcgaacga atatgtgcga gtagctctgc cttactcgct
     1681 ctcggattta aaaataattg gatgtctgtc tgatattcta aatacaaccg atagcccact
     1741 accagtatca gaatgattcc caacgagatc aacatcaatc ggattatttt ctttttcata
     1801 tttcctcctg aaaaaaatct atattcatta aataatagtt taacataaaa acaaaaaagc
     1861 accgaaatcc aacataggat ctcagtgctt ttaaa
//
LOCUS       DACDYM010000168         1853 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDYM010000168 DACDYM010000000
VERSION     DACDYM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.179"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            147..1352
                     /locus_tag="IWM59_002749"
     CDS             147..1352
                     /locus_tag="IWM59_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9980723.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            1368..>1853
                     /locus_tag="IWM59_002750"
     CDS             1368..>1853
                     /locus_tag="IWM59_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980724.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SV"
BASE COUNT          611 a          316 c          426 g          500 t
ORIGIN      
        1 ggtaaatcaa tgacaaactc agaattagaa tccattcctc aacaaaatca aggagacgct
       61 gtgatcaatt ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa
      121 ttaaaattgt ttgatggagg aaaataatgg aaaaccaaaa tgaaagttta atcaagcagt
      181 atgttaagcg tcgtgcaaag cgacgcttat ttttgtggtt gtttggtact tctgcaggtt
      241 tgattactat tttgattact gtgttcgtga ccctttttct aatacttgcg gcgggttcga
      301 ttgataattc tgattcagat tcaagtagtg gtggcgaagc ttttacagga gaatattcgg
      361 aaggcttacc gatttataaa gaaatcaaag gaagaggccc tttttctgat gagattgcgc
      421 aatatgctgt aggtgcagca gtgaaatata aattattacc aagtgtgatc ctttcgcaat
      481 acggttatga atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc aactattttg
      541 ggattacttg gtttgatggt tgtctgtttc ctaaaggaac tgcgcgagga atcggtggta
      601 ttgaaggtgg ctggtacatg aagtttccca actcaaaagc agccttctcc tattacggct
      661 ttatggtagc cacacaatcc aattttaatg catgtgtagg aaataaaagc cctggcgcaa
      721 gcttattgat cttaggacga ggtggttatg ccgctgcagg aattactgaa gatagtccgt
      781 attatacagg ttgtatgtcc attatcactt ctaataaatt aaccgaatat gatgaattcg
      841 ccatcaaaca ttggggtgaa ggtggcaata acaacggcac gatcactggt gaatggacga
      901 atccattccc tggcagcagt ttagataaaa gttccttcag tggtggtcaa ttatttggaa
      961 caaatccagg cggtgaattc cgtccgaatg gcttccatga tggattagac tttggttcag
     1021 tcgatcatcc aggaagtgaa attcacgcgg tgcatggagg aaaagtcgtg tacgttggca
     1081 atcctggtat ttcaggttta ggtgcatgtg tgatcgtgat taattacgac ggtttgaata
     1141 tggtatatca agaatttgcg aatagtacag gaaattctcg tgtgaaggtt ggcgatcaag
     1201 tgaaagttgg tcaagtaatc gggatacgag atacagcaca cttgcattta ggtttcactc
     1261 gaatggattg gcgtcaagca caaggacacg cattcacaga cgatggtaca tggattgatc
     1321 cattaccatc tttgaatagc agcaaaaaat agatcaggag aaaaaatatg gaaaataaaa
     1381 ctttaaaaat cgtcttaaca gctgcaatta gcgttagttt aatcggcaat atcgcgttgt
     1441 tttggcagct gaaagaagaa caagagacaa atacctttaa aattgaagaa gtggctaaaa
     1501 acgcgtctga gaagctcaaa aaaaattcct ctgaggcaca ggcgaagatc gacaagttaa
     1561 atcaaaccat caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa gatggcacaa
     1621 aagcaaaaga ggaagtaact gaagcacaaa gaaagacggc agaagagttc ggtcaattgg
     1681 ccattgataa atcattaagt agaaatgaat taacggaaaa actaaaagat gtcgcaaccc
     1741 aagaagtgat cgacaaatta agtcctgcag acgatgatca ccaacatgat gattacggaa
     1801 gttactcctt tacattaggt gacgtgcaaa cttatgctca aacaagcagt gta
//
LOCUS       DACDYM010000169         1791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDYM010000169 DACDYM010000000
VERSION     DACDYM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.180"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            137..748
                     /locus_tag="IWM59_002751"
     CDS             137..748
                     /locus_tag="IWM59_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9980725.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="IWM59_002752"
     CDS             821..1750
                     /locus_tag="IWM59_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9980726.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
BASE COUNT          587 a          253 c          336 g          615 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa a
//
LOCUS       DACDYM010000170         1719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDYM010000170 DACDYM010000000
VERSION     DACDYM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.182"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            10..375
                     /locus_tag="IWM59_002753"
     CDS             10..375
                     /locus_tag="IWM59_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9980727.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            376..>1719
                     /gene="aph(2'')-I"
                     /locus_tag="IWM59_002754"
     CDS             376..>1719
                     /gene="aph(2'')-I"
                     /locus_tag="IWM59_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP9980728.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYY"
BASE COUNT          711 a          130 c          296 g          582 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      481 ggtagagata aaaaatatac attagaatca ttaaaaaaac attatacaga gccttgggaa
      541 gatgaagttt ttagagtaat tattgaatat aacaatgttc ctattggata tggacaaata
      601 tataaaatgt atgatgagtt atatactgat tatcattatc caaaaactga tgagatagtc
      661 tatggtatgg atcaatttat aggagagcca aattattgga gtaaaggaat tggtacaaga
      721 tatattaaat tgatttttga atttttgaaa aaagaaagaa atgctaatgc agttatttta
      781 gaccctcata aaaataatcc aagagcaata agggcatacc aaaaatctgg ttttagaatt
      841 attgaagatt tgccagaaca tgaattacac gagggcaaaa aagaagattg ttatttaatg
      901 gaatatagat atgatgataa tgccacaaat gttaaggcaa tgaaatattt aattgagcat
      961 tactttgata atttcaaagt agatagtatt gaaataatcg gtagtggtta tgatagtgtg
     1021 gcatatttag ttaataatga atacattttt aaaacaaaat ttagtactaa taagaaaaaa
     1081 ggttatgcaa aagaaaaagc aatatataat tttttaaata caaatttaga aactaatgta
     1141 aaaattccta atattgaata ttcgtatatt agtgatgaat tatctatact aggttataaa
     1201 gaaattaaag gaactttttt aacaccagaa atttattcta ctatgtcaga agaagaacaa
     1261 aatttgttaa aacgagatat tgccagtttt ttaagacaaa tgcacggttt agattataca
     1321 gatattagtg aatgtactat tgataataaa caaaatgtat tagaagagta tatattgttg
     1381 cgtgaaacta tttataatga tttaactgat atagaaaaag attatataga aagttttatg
     1441 gaaagactaa atgcaacaac agtttttgag ggtaaaaagt gtttatgcca taatgatttt
     1501 agttgtaatc atctattgtt agatggcaat aatagattaa ctggaataat tgattttgga
     1561 gattctggaa ttatagatga atattgtgat tttatatact tacttgaaga tagtgaagaa
     1621 gaaataggaa caaattttgg agaagatata ttaagaatgt atggaaatat agatattgag
     1681 aaagcaaaag aatatcaaga tatagttgaa gaatattat
//
LOCUS       DACDYM010000171         1682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDYM010000171 DACDYM010000000
VERSION     DACDYM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.183"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IWM59_002755"
     CDS             200..742
                     /locus_tag="IWM59_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980729.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="IWM59_002756"
     CDS             898..1347
                     /locus_tag="IWM59_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9980730.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>1682)
                     /locus_tag="IWM59_002757"
     CDS             complement(1377..>1682)
                     /locus_tag="IWM59_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9980731.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          636 a          240 c          324 g          482 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gt
//
LOCUS       DACDYM010000172         1673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDYM010000172 DACDYM010000000
VERSION     DACDYM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.184"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(341..913)
                     /locus_tag="IWM59_002758"
     CDS             complement(341..913)
                     /locus_tag="IWM59_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9980732.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(929..1534)
                     /locus_tag="IWM59_002759"
     CDS             complement(929..1534)
                     /locus_tag="IWM59_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9980733.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          486 a          352 c          243 g          592 t
ORIGIN      
        1 acaatcaaca ccgtagggat atggagataa aatcaataga gtcaatagat tatttatcga
       61 taataacact aaaaaagcca cgttcctaaa cgtgacttac gctgaacgat atgtgttgtt
      121 tggtagacga acaaatcgtt cattataaat atatgatggt atcctaatat agtaagcgtg
      181 gctagcatac ctttaattac tttacctttt acagttacca tacacatttt ttgagcagaa
      241 atcaagtaaa taaaaaaata ttatatttca caacatgcta atagactcat atctagaacg
      301 cattaagtat ttacctgtct ggttcgctga gacttctttt ttaactttct tcaattttct
      361 tctttattct aaaaagcgta ctgcgactga aaccggttat agcttcaact tccttatacg
      421 atttacctga agtaagcagt tcatatgcgt gcctcttctt cggtgtgatt gttgctttcg
      481 gacgtccttc tctgaaatta ggattattct ttcttgcaaa catttttcct tcttgggtac
      541 gttccacaat catatcacgt tccatttctg ctacactcaa caacgtccgt gtcaccattc
      601 gccccatgga cgtattttca atcgttccta gattcaaaac cttgatcgtt acgttatcat
      661 cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt acgagcaaat cgatcaagct
      721 tcgtaacaac tatttcatct ccaggcttca gaccagccaa cagctgattg aattttggcc
      781 gatcccgttt tgtacccgta aatttttctg aaaaaatatt cttcttctct acgcagttct
      841 caagcaactg ttgaatttgt tcttttgtac tttgagaagc ggttgagaca cgtgcatatc
      901 catatttcgt catttggtcc acctactttt acaaatcatc tatcgtatac tcagaatacc
      961 cttcatagaa atagctgaca tcgattcctt tcatatataa ctcgcgatcg tcaattttat
     1021 cagtcagagc attggaaatt agatagcgta tttccaaatc gtttatggga cttcgctcca
     1081 ttgcagaaag atagtctgct ttatcgatta agttccaatc tacaactttt tgcaattctt
     1141 cctttaaaat gagatccagc caaattctag tacttcggcc atttccttct cgaaatgggt
     1201 gagcgatatt catctccaca tatttcttga taatctcttc aaaagaagtt tgtggcatct
     1261 gatcaatatg ctgtaacgaa tgttctaaat acataactgg tgcaaatcga aaatttcctt
     1321 ttgcaatatt gaccgatcgg atttttccgg caaaatcgta aatatccgaa aatagaaatt
     1381 catgaatttc agataaacct ttaaatgtac caacttcgat ttcatttatt tttccacaat
     1441 cgtacaattc tttcgctttt tgtttactga gtttttcttc aaccttagcc aactcaactt
     1501 gattggttaa tcccaattta ttctctaaaa tcatgcgaaa tctccttaac tttatgacat
     1561 aagtttatga aacactctca accccattat atcgcgattt tgtttatgtg tcaaaaacta
     1621 ttaagtttct gacccccgat agatttttca agtgtctcta ttttgcacag tct
//
LOCUS       DACDYM010000173         1647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDYM010000173 DACDYM010000000
VERSION     DACDYM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.185"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1118
                     /locus_tag="IWM59_002760"
     CDS             <1..1118
                     /locus_tag="IWM59_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9980734.1"
                     /translation="GLITILITVFVTLFLILAAGSIDNSDSDSSSGGEAFTGEYSEGL
                     PIYKEIKGRGPFSDEIAQYAVGAAVKYKLLPSVILSQYGYESAFGTSASARNDLNYFG
                     ITWFDGCLFPKGTARGIGGIEGGWYMKFPNSKAAFSYYGFMVATQSNFNACVGNKSPG
                     ASLLILGRGGYAAAGITEDSPYYTGCMSIITSNKLTEYDEFAIKHWGEGGNNNGTITG
                     EWTNPFPGSSLDKSSFSGGQLFGTNPGGEFRPNGFHDGLDFGSVDHPGSEIHAVHGGK
                     VVYVGNPGISGLGACVIVINYDGLNMVYQEFANSTGNSRVKVGDQVKVGQVIGIRDTA
                     HLHLGFTRMDWRQAQGHAFIDDGTWIDPLPFLNSSKK"
     gene            1134..>1647
                     /locus_tag="IWM59_002761"
     CDS             1134..>1647
                     /locus_tag="IWM59_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980735.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVF"
BASE COUNT          539 a          280 c          390 g          438 t
ORIGIN      
        1 caggtttgat tactattttg atcactgtgt tcgtgaccct atttctaata cttgcggcgg
       61 gctcgattga taattcggat tcagattcaa gcagtggtgg cgaagctttt acaggagaat
      121 attcggaagg cttaccgatt tataaagaaa tcaaaggaag aggccctttt tctgatgaga
      181 ttgcgcaata tgccgtaggt gcagcagtga aatataaatt attaccaagt gtgatccttt
      241 cgcaatacgg ttacgaatca gcgtttggta catcggcgtc cgcaagaaat gatctcaact
      301 attttgggat tacttggttt gatggttgtc tgtttcctaa aggaactgca cgaggaattg
      361 gtggcattga aggtggctgg tacatgaagt ttcccaactc aaaagctgca ttttcctatt
      421 acggctttat ggtagccaca caatccaatt ttaatgcttg tgtaggaaat aaaagccctg
      481 gcgcaagctt attgatctta ggacgaggcg gttatgccgc tgcaggaatt acggaagata
      541 gtccgtatta tacaggttgt atgtcaatta tcacttctaa taaattaacc gaatatgatg
      601 aattcgccat caaacattgg ggtgaaggtg gcaataacaa cggcacgatt actggtgaat
      661 ggacgaatcc attccctggc agcagtttag ataaaagttc cttcagcggt ggtcaattat
      721 ttggaacgaa tccaggcggt gaattccgtc cgaacggctt ccatgatgga ttagattttg
      781 gttcagttga tcatccagga agtgagattc atgcggttca tggcggaaaa gtcgtgtacg
      841 ttggcaatcc tggtatttca ggtttgggcg cgtgtgtgat cgtgattaat tacgatggct
      901 tgaatatggt gtatcaagaa tttgcaaata gtacaggaaa ttctcgagtg aaagtaggcg
      961 atcaagtgaa agttggtcaa gtaattggga tacgagatac agcacacttg catttaggat
     1021 ttactcgaat ggattggcgt caagcacaag gacatgcatt cattgatgat ggtacatgga
     1081 tcgatccgtt accatttttg aatagcagta aaaaatagat caggagaaaa aatatggaaa
     1141 ataaaacgtt aaaaatcgtc ttaacagctg caattagcgt tagtttaatc ggcaatattg
     1201 cgttgttttg gcagctgaaa gaggaacaag cagcaaatac ctataaaatt gaagaagtgg
     1261 ctaaaaacgc gtctgagaag ctcaaaaaaa attcctctga ggcacaggcg aagatcgaca
     1321 agttaaatca aaccatcaat gagaaaaacc aagaaatcga tcagctgaag caacaagatg
     1381 gcacaaaagc aaaagaggaa gtaactgaag cacaaagaaa gacggcagaa gagttcggtc
     1441 aattggccat tgataaatca ttaagcagaa atgaattaac ggaaaaacta aaagatgtcg
     1501 caacccaaga agtgatcgac aaattaagtc ctacagacga tgatcaccaa catgatgatt
     1561 acggaagtta ctcctttaca ttaggtgacg tgcaaactta cgctcaaaca agcagtgtaa
     1621 aagaggaaca aaattttgtc gtatttg
//
LOCUS       DACDYM010000174         1608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDYM010000174 DACDYM010000000
VERSION     DACDYM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.186"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IWM59_002762"
     CDS             complement(52..507)
                     /gene="tnpA"
                     /locus_tag="IWM59_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9980736.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            750..1608
                     /locus_tag="IWM59_002763"
                     /pseudo
     CDS             750..1608
                     /locus_tag="IWM59_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          522 a          307 c          305 g          474 t
ORIGIN      
        1 ggggattttt aaaagaccgc caactgctca tcttcactca cttacttctc tttatttttt
       61 aaatggttct acgtactctc ttttacttat aatatatcga atgcgatcat cagcttcttg
      121 ttcacggatg tatttttcat ttgtcttttg attcaatccg actgtactca catagtatct
      181 cttcgaccaa aaggatttgt ttccgtattt atatttgaga tttgcgtgct gctcgtggat
      241 tagtaccgca ctacgacctt tcagataccc cataaaactg gatatacata tttttggtgg
      301 tactcgaaca agcatgtgaa tatgatctag cattgcatgt gcttctatga tttccacatc
      361 tttcatttcg cacaacttcc taagtatttt tccaagatct tttcgtaatt gcccatagat
      421 tacttttcgt tgatattttg gtgtaaaaat aatatgatac ttacaattcc aacgagtata
      481 tgctaaactt ttatcgtcat ttgacatgaa aaaactcctt tcgatctgat gagcggttgg
      541 tagccactca ctagtatatc gaaaggagtt tttatcgcta aagatacttc ttccacgcgt
      601 agaaacgcgc ggttttcttg tttcgcacgc tttacgcaca aaactgactg aagtcattaa
      661 gaaaataaaa gggtctaaaa cttttaagtt ttgtaccgac ccccaaaagt tagaccaaaa
      721 aatctaactt ttggaggtcg gtatttttta tggctaagta tacttttgaa cttaaattaa
      781 agatcgttca tgattacttg gatggtaaag gcggaagtga ttatctggca aagaaatatt
      841 ccattaaggc tccatcacaa gtgaagcgct ggatcaatgc ctatcaagaa tttggtgaag
      901 agggtctagt aagaaagcga caaaagaaaa aatattctgt tcaatttaag ctggatgcga
      961 tagagttata tctaacaagt gagctgtcct atcgtgaagt tgccaacaca cttaacatga
     1021 acaaccctaa tttgatcgct agttggatgc gtcaattccg tgagggtggg atcgatggac
     1081 tctcaaaaac gaaaggatgt ccacctatct tgtctaagaa aaatgaaccc aaaaataaga
     1141 aaaaatctac aactaaagca acgtctaaag aacgtgaacg catcgaggag ttggagaaac
     1201 gagtccgttc ccttcaaatt gaaaatgcct ttttaaaaga attgaggaag ctgcgaaaac
     1261 aggaagccca acaacgacga atgaagcaat cgcacgaatc atcgcaagcc tccgaggacc
     1321 gtttaaatta gttgagcttc tagaaacttt aaagttccct aaggcaatct ttatgtactg
     1381 gcaaaaacga ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaagt
     1441 tcgggagaag cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg
     1501 tttctctatc aataaaaaga aagttcagcg cctgatcaag aagctaggaa taaaagtaac
     1561 gacatacaca cgtaaatcac ggcgctacaa ttcttatcga ggacaagt
//
LOCUS       DACDYM010000175         1509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDYM010000175 DACDYM010000000
VERSION     DACDYM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.188"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            101..868
                     /locus_tag="IWM59_002764"
     CDS             101..868
                     /locus_tag="IWM59_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9980737.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="IWM59_002765"
     CDS             1060..1458
                     /locus_tag="IWM59_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9980738.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DACDYM010000176         1469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDYM010000176 DACDYM010000000
VERSION     DACDYM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.190"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            84..494
                     /locus_tag="IWM59_002766"
     CDS             84..494
                     /locus_tag="IWM59_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9980739.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            508..864
                     /locus_tag="IWM59_002767"
     CDS             508..864
                     /locus_tag="IWM59_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9980740.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          540 a          211 c          271 g          447 t
ORIGIN      
        1 ttttttatat tttattagct aataattggt attttaaaag aaattactgt agaattggat
       61 tataattaaa aaggagtgag aaaatgataa aagtatatac ttcgcctagc tgtacttcct
      121 gtagaaaagc aagagcatgg ttgcaagcga atcaacttga atttgaagag aagaatattt
      181 ttgccgaacc gttaacagaa agtgagatca aaaagatctt ggcagcgact gaaggaggag
      241 ttcaagatat tatttccact cggtcgaaaa tttacgaaaa gctgggcata gattttaacg
      301 aattaacttt aaagcaaatg atcgtcttat tcaaagaata tccttcatta ttgagacgac
      361 ctattttgat tgatgaaaaa agaatactaa tcggatttaa tgaagatgag atccggtcat
      421 ttgtccctcg tgaaattcga tgtgttgcca gaaatctgat ctatttacaa aacaataaca
      481 tcttaaattc atagggaggg agccttcgtg gaagtgaaag tctatggatc agcgcactgt
      541 gtaacaagtc tgaaggcgaa acaatggtta gaacaccatc aattgcctta tcaatttgtt
      601 gacttagaag aacgcgatct aagtacagaa gaagcagaag aattatgcag ttttgaagaa
      661 gtgcaagcag aacagctatt tgcgacttgg tctgaggctt ttcacaacat tgcgattgac
      721 ctttcctgtg taggaaaaaa acaactgatt tttttatgca cacaacatac caatctcctc
      781 agacgtcctt tgattattat cgatgaagtt ctatttattg gatacaatga gcagttgatg
      841 gaacagctta tcttaaataa atagacttta actgttttaa tcaagtcact gagcatttca
      901 tacagttgta acagtcgaac acttagcatc aagcgtgtta tgtgttcttt ttttgacttt
      961 atttttctac tatagagaca tgctcaattt atgtggtcaa aattcaaatc taggtgggga
     1021 tatagagtta aaaatatatt gacatgtgta aatttacatg atatattcat ttctgttgtt
     1081 ttaagtagca aagcaataaa atagtatttt aaaaaacata taaaatagtt gttgacatgt
     1141 tagaaatgac atgataagat aactgagttg ttaaaaaaga accacttgtg aagcttgatt
     1201 ctatcgaaaa aaacttcaaa aaagttcttg acaaataaca aatgataagt tatgataaat
     1261 gagttgctgt aagatttcga aaaacgaaat caaaaaaact tttaaaaaag agttgacaat
     1321 tacttgtctc tctgatatga tataaaagtt gctacggcag ccaaaaacga atcggggaac
     1381 actcctgatg aagtagacct ttgaaaactg aacaaagtaa gacaaaccaa atgtgtaggg
     1441 cgtcttgatt caattcaaga caacaaaca
//
LOCUS       DACDYM010000177         1466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDYM010000177 DACDYM010000000
VERSION     DACDYM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.191"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1014..1304)
                     /locus_tag="IWM59_002768"
     CDS             complement(1014..1304)
                     /locus_tag="IWM59_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980741.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          469 a          288 c          205 g          504 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tataga
//
LOCUS       DACDYM010000178         1458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDYM010000178 DACDYM010000000
VERSION     DACDYM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.192"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..226
                     /locus_tag="IWM59_002769"
     CDS             <1..226
                     /locus_tag="IWM59_002769"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP9980742.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IWM59_002770"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IWM59_002770"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IWM59_002771"
     CDS             533..1336
                     /locus_tag="IWM59_002771"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9980743.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT          466 a          261 c          347 g          384 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggacagaagc gtttaact
//
LOCUS       DACDYM010000179         1457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDYM010000179 DACDYM010000000
VERSION     DACDYM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.193"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            109..1041
                     /locus_tag="IWM59_002772"
     CDS             109..1041
                     /locus_tag="IWM59_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP9980744.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1457
                     /locus_tag="IWM59_002773"
     CDS             1113..>1457
                     /locus_tag="IWM59_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980745.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          228 g          420 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaagat
//
LOCUS       DACDYM010000180         1457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDYM010000180 DACDYM010000000
VERSION     DACDYM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.194"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            158..>1457
                     /locus_tag="IWM59_002774"
     CDS             158..>1457
                     /locus_tag="IWM59_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980746.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAG"
BASE COUNT          478 a          204 c          304 g          471 t
ORIGIN      
        1 ttgagaatga gacggctaaa aaattgttaa ttcaatttta cgaagaacaa gcaacggatc
       61 gtcttcaaga atatgttgaa aagttgatca aaaatgaaga aatttctgaa atgattagga
      121 aacggctgtt attggaagct gaaaaggcgg tgaaagaatg atcattggag gaattttctc
      181 tggcatagcc gactggtggt caggtttatt cggagaaggg gatgcagatc ccaaaaccgt
      241 aacgaaagtc ttacaagatt atggtaatta tctgcagtat caagatgtat tcagttggat
      301 ttgtaatact tttttatggg gattgatcaa agggttatat aatctcaata ctttattaga
      361 aaaaacaatt tatcaaagct ttgatttaaa agacttattg aacgccgctg gtgttaatga
      421 actatttcaa agtttgatta gtaaggtatt ggctcttttt tgtgtcgtca cattaattta
      481 tttaggcttg aaatttttta tgagtaaaca cccaccgaaa gtcaaaaata tcttggttaa
      541 tttatttatg gccatgatct tgatttttgg tggtagttct ttaatcgatg aagggttaaa
      601 tatcagtaaa aacttttatg gagacgtcac tgcagcaaat aaagcagata aaacgggttc
      661 acctgctttt caaattattc aaaataatgt gattgatgtc agtacattac tagcgactga
      721 cccgaataaa gtagaatcct taccaacaga taaaagaaat gctttaacag cgaaaaattt
      781 caaaacagcg aatatcaatg ggattattac acctgatcaa gctaagaaaa tgtctgaatc
      841 aagtgatatt agtacagtac aaaaagatcg gttaaaggca ttacagtatc gactagagtt
      901 agacgataat gggaaagaaa tccctgttca aattagtgat gaaggtttag caaaatatgt
      961 gtatacgtca ggctatcgtc gatacattag tagtccaggg attattttgg ctggggaatc
     1021 ttctttgtcg gtagcctatt tatttatttt gtttacaatc gttacttgca ttattgagct
     1081 agtatttaaa aaattctatt tagtaattgc cgcgtcaacg gactttgaga caggacaacg
     1141 gatgaaaagt gcgatagaag atatttctca atccttcttg ttattggcgt ttacgatttt
     1201 agaattacga atctatacat taatgctttc aggtatcggt gatttgcatg cggtaggaaa
     1261 gatcaatgga ttcttgtatg ttgttgcttt aattgtgcta actattgcgc tattcaaagg
     1321 ctctcaatcg gttacgaaga ttttcggtgt tgatacgagt ctgaaaaacg gcagcaattc
     1381 tcttcttagc atgtttgcct taggaaatat tgcgaaaaat gtgggtagcg gagctaaaaa
     1441 ccttgcaagt gctggta
//
LOCUS       DACDYM010000181         1418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDYM010000181 DACDYM010000000
VERSION     DACDYM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.195"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWM59_002775"
     CDS             complement(150..1337)
                     /locus_tag="IWM59_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9980747.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttca
//
LOCUS       DACDYM010000182         1391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDYM010000182 DACDYM010000000
VERSION     DACDYM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.196"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(142..813)
                     /locus_tag="IWM59_002776"
     CDS             complement(142..813)
                     /locus_tag="IWM59_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9980748.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(873..1082)
                     /locus_tag="IWM59_002777"
     CDS             complement(873..1082)
                     /locus_tag="IWM59_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980749.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(1149..1388)
                     /locus_tag="IWM59_002778"
     CDS             complement(1149..1388)
                     /locus_tag="IWM59_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980750.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT          383 a          316 c          185 g          507 t
ORIGIN      
        1 tgtacttgat acccctgaaa gcagcctgca atgattccta acattccaac aagcattcca
       61 gcttttttta cataccgatt tattgccatt ttttactctc ctcatttcat atttcaacga
      121 ctcgcgttga ttaaaacctt gttattgtag ctctccctgt accacgtatc tttcttttcc
      181 ttgtgacgta taaacacaag taaacaacgt taataagggt ttctctcctt tctttggcaa
      241 agacaagaaa gagttctctt caggaacgcc atcgattaat ttaaagtacc caggtacgat
      301 aaatttacgc gttaccttgt aacgatagac gccatcgtta ctaactaagt ccatcacctc
      361 ccctaatgac aacttttgaa tatcagaaaa taaaacacct ggcatttcca tgttatgacc
      421 tgctaggaca tagtttcctt tgcccatttc ggcatcttca taaaaataag tagccgctcc
      481 aagactcaaa gtgtattgat ttgccccttt aaaaattggc aatgctaaac caagtgcagg
      541 aatctgaatt tgtgcaaata cttgtttatt gacgcccatt tcgattgctt ctttttgtat
      601 ggacaacatt tgccccaaag acatatcatt attgacgttc gttgcatcct ccttataact
      661 ctttgcatgt gcattgattt gatctacggg catctttttt tctatatttt gcttaaacac
      721 ctctccttca tgtttttggt agctaacata gccaaacaca ctgcaaaaga cgattaaagt
      781 gactcctaaa aaaccaaata accatttttt catccatctg cctcctttca gacaaaacaa
      841 aaaaactttt tctcatttcc atagaaaaag ttttaaaaat gaattctata agttcgtctc
      901 aactgttcaa agtttttcat tgaacctaac aaacaagctt tctcttttcc tgttaactgc
      961 atatcattga tatattgcgc gacagataat cttttaaggt gtttttgctt catcgttttt
     1021 agtaactttt cttccatttc tttcgacact ttttttctgt tttcgatttc ttcataattc
     1081 atgagttttc ctaccttcct tgttttttgc aaaaataaaa aagcctatga tcacatacgc
     1141 tctacacttc agtcttgggt tatctttttc tttcgtttaa ttactggccg tgctggtttc
     1201 aaatccttga ttccattatg ggttctccaa taatcgaatt cttttcgcaa tctttctttt
     1261 tccatatctg ttcctttaga taaggctttg aatacttctt taggtgaata accacgagct
     1321 aacagctgta cataccttcc ttttgtcaaa gtatccatat ccatcgcctc taaaactggt
     1381 cgcgccatct a
//
LOCUS       DACDYM010000183         1381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDYM010000183 DACDYM010000000
VERSION     DACDYM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.197"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(487..978)
                     /locus_tag="IWM59_002779"
     CDS             complement(487..978)
                     /locus_tag="IWM59_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980751.1"
                     /translation="MPDLDFTTNTHLHFLEAYINFYMTTPTFDVKTTLQRLLIVFTDK
                     IATPANCLQFFNADWCPLSQEISFSHDIETIWVLQRTLTVTGIKDVQVSQFIENLGTQ
                     VATKGFDNNKIICDHSNTLQTSTRKTWWTLAEAIIGFYNLYQLTNSKVYYELATHHFE
                     MVE"
BASE COUNT          431 a          229 c          301 g          420 t
ORIGIN      
        1 aggaactttg agatattctt taggatatta aaattaacta gcaccacggg taaaataata
       61 aattctgata ttgaaataaa gatagaaaat taaaagggat tacctttatt ttaattcaaa
      121 aattatgaaa aatagggtgg agttgctgga gagaatcaat taggttgtag tattaaggat
      181 aaccaagagc taaagaacca tttgcgcata tatttgcatc taagcagaaa ttactaaaat
      241 aaactatcaa aaagccaact gttctttagt tggctttttg cgatgtcact acaattcgaa
      301 tgattctcta gaagtcaggt cgtcctgttt ttcttaccta gataggcgtg caattgtttc
      361 tgtgtacata cgacaggtat ggtaaggggc ctttccaagc gttggcgata tcttctgtag
      421 aatctggtgt ccatcattga tgaggtaggc ataccattca ctgttagatc gagtatccac
      481 aaagtttcat tctaccattt cgaagtggtg agtagccaat tcataataga ccttactatt
      541 agttaattga tacaggttgt aaaagccgat gattgcttct gctagcgtcc accaagtttt
      601 tcgagtcgag gtttgaaggg tatttgaatg atcacaaatg attttgttgt tatcgaagcc
      661 tttggtagct acttgagtgc caagattttc aataaattga ctgacttgca catctttgat
      721 accagtaaca gtcaaagtcc gttgcaatac ccaaatggtt tcaatgtcat ggctaaaact
      781 gatttcttgg cttagaggac accaatcagc attgaaaaat tggaggcagt ttgctggggt
      841 cgcaatctta tctgtaaaga caatgagtaa tctttgcaag gtagttttta catcgaatgt
      901 tggcgtcgtc atgtagaaat ttatgtatgc ctctaaaaag tgcaaatggg tattagtggt
      961 aaagtctaga tctggcaaat cgctgggtcc gattgattgg gagggaacgg gctaccagtt
     1021 gtgggaaaaa tcctcctgat aaccatttgt aagagggtca taagacttct tttcgagaaa
     1081 actaaagagc gtggttgcca tttttaatgc agacagatca tgaaagtcta agtaatactc
     1141 gctaagaaca tagagggcaa aactattagc ataagtggtt ttattgggat cagagagtat
     1201 ttcagttgcg gttagctccc agaagatccc ctgggttttc ctggtcccaa cagacttacc
     1261 ggaaaaattc ataggcagga ttcgcagcag tctttaggct agctaaaaaa gactcagtta
     1321 aggatttgtc ttgcaaaatg cggcagcaat gactaaaact ccataaatac cgggcgttca
     1381 t
//
LOCUS       DACDYM010000184         1378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDYM010000184 DACDYM010000000
VERSION     DACDYM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.198"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..174
                     /locus_tag="IWM59_002780"
     CDS             <1..174
                     /locus_tag="IWM59_002780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980752.1"
                     /translation="LARGYSPKEVFKSLSKGTDMEKERLRKEFDYWRTHNGIKDLKPA
                     RPVIKRKKKITQD"
     gene            241..480
                     /locus_tag="IWM59_002781"
     CDS             241..480
                     /locus_tag="IWM59_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980753.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            566..1237
                     /locus_tag="IWM59_002782"
     CDS             566..1237
                     /locus_tag="IWM59_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9980754.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
BASE COUNT          511 a          183 c          299 g          385 t
ORIGIN      
        1 ttagctcgtg gttattcacc taaagaagta ttcaaatccc tgtctaaagg aacagatatg
       61 gaaaaagaaa gattacgaaa agaattcgat tattggagaa cccataatgg aatcaaggat
      121 ttgaaaccag cacggccagt aattaaacga aagaaaaaga taacccaaga ctgaagtgta
      181 gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa caaggaaggt aggaaaactc
      241 atgaattatg aagaaatcga aaacagaaaa aaagtgtcga aagaaatgga agaaaagttg
      301 ctaaaaacga tgaaacaaaa acaccttaaa agattatctg tcatgcaata tatcaatgat
      361 atgcagataa caggaaaaga gaaagcttgt ttgttaggtt caatgaaaaa ctttgaacag
      421 ttgagacgaa cttatgtgaa aaaaagttct aattgtcaac tattgttaga agtctcatga
      481 cattcattaa tagaattcat ttttaaaact ttttctatgg aaatgagaaa aagatttttt
      541 gttttgtctg aaagggggta gctggatgag aaaatggtta tttggttttt taggagtcgc
      601 tttaatcgtc ttttgcagtg tgtttggcta tgttagctac caaaaacatg aaggaaaggt
      661 gtttaagcaa aatatagaaa aaaagatgcc cgtagatcaa atcaatgaac atgcaaagag
      721 ttataaggag gatgcaacga acgtcaataa tgatatgtct ttggggcaaa tgttgtccat
      781 acaaaaagaa gcaatcgaaa tgggtgtcaa taaacaagcc tttgcacaaa ttcagatacc
      841 tgcacttggt ttagcattgc caatttttaa aggggcaaat caatacactt tgagtcttgg
      901 agcggctact tatttttatg aagatgccga aatgggcaaa ggaaactatg tcctagcagg
      961 tcataacatg gaaatgccag gtgttttatt ttctgatatt caaaagttgt cattagggga
     1021 ggtgatggac ttagttagta acgatggcgt ctatcgttac aaggtaacgc gtaaatttat
     1081 cgtacctgag tactttaaat taatcgatgg cgttcctgaa gagaactctt tcttgtcttt
     1141 accaaagaaa ggagagaaac ccttattaac gttgtttact tgtgtttata cgtcacaagg
     1201 aaaagaaaga tacgtggtac agggagagct acaataacaa ggttttaatc aacgcgagtc
     1261 gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa tcggtatgta aaaaaagctg
     1321 gaatgcttgt tggaatgtta ggaatcattg caagctgctt tcaggggtat caagtaca
//
LOCUS       DACDYM010000185         1372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDYM010000185 DACDYM010000000
VERSION     DACDYM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.199"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            37..1338
                     /locus_tag="IWM59_002783"
     CDS             37..1338
                     /locus_tag="IWM59_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9980755.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          446 a          275 c          253 g          398 t
ORIGIN      
        1 ttgaatcacc acaacacaat caaaggagcg ttctgtatgc ctgatcttca tcatatcaaa
       61 ctactacttg gaatcaaaga taaaaacatt ttcatcacaa atgtggaatc gaagagcatt
      121 aagaatatca agagtcttgt tgtttctgcg acgttaagta aagaaattcg tcgctgtcct
      181 ttgtgtaaac agatgaatca tgaaggcatg atcgttaaga acggaaagaa aaaatcgctc
      241 attcaactta ataaatgtgc gaaccaactg acttatcttg ctttagcgaa acaacgctac
      301 cactgtcgtg gctgccacac ctattttacg gcgaatacct acattgtcga tagaaactgc
      361 tttatcgcta aacaagtgcg ctataaaatt ttagaagagc taactgaaaa acaagcaatg
      421 acgacaattg ctaagcattg cggcgtttct tggtcgaccg tttctcggac actcgcttct
      481 ctgatcccca tgacaaaagt aaaaagaaat tggttaccgc gttgtttatt aatggatgaa
      541 tttcgttcac tgaaaaatca agtcggtccc tactcctttt cttgtatgga tggcgatact
      601 ggaaaattac tggatattct tccttcacgg aagaaaaagg ctctcgtttc ttattttatg
      661 caatttgaac gacgcgcacg attgaaggta aaggtcatcg tgacggacat gaatgtttct
      721 tatgcctctc tgataaagga atgttttcca aaggcaaaac tcgttgtgga tcgttttcac
      781 attgtcaaac atctcattcg taaatttgag gatattcgcg tacgaatcat gaaaagcttt
      841 gatcgaaacg atccaacaca agccaaatat tatcgacaat taaaggcgtt gtctcggctt
      901 ctgatcaaac gacaggacaa gcttgtttat gacaaatgga ccaaatggcg caattttggt
      961 tgggcgtatc tcaccgaaag tgaagtggtg gatcgtctgc tttcgatctc tgacgaatta
     1021 cgaatcgcct atttttacta tcaagaaatt cttcaagcgt ttcacgacaa agaggcggac
     1081 actttcttta aacttgtaag aacgatgccc aattccgttc caaaagaact gcattctata
     1141 aaaaaagctt ttataaacta tgaatccggc attcgtttag ccctcgaatt gccttattca
     1201 aatgccaaaa tagaaaatct gcatacccac atcaaagcat tgaaacgagt cgcttatggc
     1261 tttcgaagct ttcggaaaat gaagaccaga attttcttac taaataatct gattacctat
     1321 gaatcaaaaa acatctaaag tagaaaagga attctacctt agatgttctt at
//
LOCUS       DACDYM010000186         1354 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDYM010000186 DACDYM010000000
VERSION     DACDYM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.200"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            33..>1354
                     /locus_tag="IWM59_002784"
     CDS             33..>1354
                     /locus_tag="IWM59_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9980756.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPT"
BASE COUNT          495 a          251 c          251 g          357 t
ORIGIN      
        1 aacattttta attaaattta ggagttgaaa aaatgaaatt atttaatcca aaaactgttt
       61 tagctattgg aacatttgct gcctcactta gtcttgctgg atctgttgac gctgcttcaa
      121 ctaacgtcat gtatcgcctt tacaatccaa ataataatga acatttttac acctctagca
      181 aaaaagaacg tgaccatcta gtaaagataa aatggggaaa ttatgaaggc cccgcctggg
      241 aagcaccgac tagcggtggc catcttgtct atcgtttgta caataaaggt cttcgtgatc
      301 accattacac agctagttgg gatgaagtga aatggttgac taaaaattac ggctggactt
      361 atgaagggcc tgcctggcgt tctgccgaaa aaaataataa accgatctac cgccttttcc
      421 ttcctggtgt aaaatctggc tcacatcact acactgcaag ttggaaagaa gtacaatggt
      481 taaccaaaga ctatggctgg aaatacgaag gtatcggttg gtatggcgct gacacctcta
      541 aacccaaagt gaataaatcg gctctagaag ctctttatac gagcgttaag ggaacgaaaa
      601 aaggtgacta taccgacaat acctggaacg cattccaaac cgctttaaac aatgctaaga
      661 aagtgttaaa tgactcaaaa gcgactcaaa agcaagtgga tagtgccaaa aataaattag
      721 attctgctta caaaggatta aagaaaaaac cagttcctac tgtgaataaa gctgaattac
      781 aagctttata caaccgagta aaatcaactg ctaaaggaga ttttacagaa gaatcttgga
      841 ataattttca gactgcttta tccaatgcta aaaaagtact agacgattct aaggtttctc
      901 aaacacaagt aaacaatgct aaaaatagtc ttgatgctgc atttaaaggg ttgcaacaca
      961 aacctgaaac gccaaaagaa tatatggtta ccattaacca tattgacaat caaacaggtc
     1021 aagtgattgc tacagatcaa gcaaaagtga cttctggttc tacttacact gctaaggcaa
     1081 aagcatttaa atatagcgaa ggtgctaaag ataattttag ctacaaagtg attggtaatg
     1141 atactcaaag caaaacaatc acaggtaata caacaatcac attcaattat aatcaagtgc
     1201 atcaaatcaa tttgttgtgc aataacaact acaaaaatga ggggcgagtt gatattttga
     1261 aagaacagat aatcaacgtc actcacggtg aaagtgtcac tttaactgct ccttctattc
     1321 aaggatatgt tttagacgat cgggtagaac caac
//
LOCUS       DACDYM010000187         1352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDYM010000187 DACDYM010000000
VERSION     DACDYM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.201"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            11..1350
                     /locus_tag="IWM59_002785"
     CDS             join(11..452,452..1350)
                     /locus_tag="IWM59_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9980757.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          565 a          175 c          235 g          377 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga
      481 acaagatttg agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt
      541 aaattgaatc tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta
      601 aaaaaattag ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta
      661 aaagaatcaa gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga
      721 tatacaatca atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa
      781 atccgaataa agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg
      841 ttaaaacgaa atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa
      901 ttcaagataa aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa
      961 ataattagtt atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa
     1021 caagcaatta aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg
     1081 cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg
     1141 tctagaaaag gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa
     1201 tcagagtttt tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat
     1261 gagtatatta ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca
     1321 gtagaatatc ggaaacagtc cattaaatag tt
//
LOCUS       DACDYM010000188         1348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDYM010000188 DACDYM010000000
VERSION     DACDYM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.203"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>1348
                     /locus_tag="IWM59_002786"
     CDS             82..>1348
                     /locus_tag="IWM59_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9980758.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIF"
BASE COUNT          458 a          260 c          256 g          374 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttt
//
LOCUS       DACDYM010000189         1334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDYM010000189 DACDYM010000000
VERSION     DACDYM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.204"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            86..1165
                     /locus_tag="IWM59_002787"
     CDS             86..1165
                     /locus_tag="IWM59_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9980759.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          359 a          357 c          369 g          249 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt
     1321 caccatgggt ggct
//
LOCUS       DACDYM010000190         1334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDYM010000190 DACDYM010000000
VERSION     DACDYM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.205"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..244)
                     /locus_tag="IWM59_002788"
     CDS             complement(<1..244)
                     /locus_tag="IWM59_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9980760.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
     gene            482..>1334
                     /locus_tag="IWM59_002789"
     CDS             482..>1334
                     /locus_tag="IWM59_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9980761.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEE"
BASE COUNT          474 a          217 c          228 g          415 t
ORIGIN      
        1 cttgccatac ctcctgaatt tgaccatcag gagtcgtaat gaaaatagta tcattttcat
       61 ctactaattc tcttaagttt aaatattgtt tactagatgt aaaattcgaa gctgctaatg
      121 acgagtcttc gcttttacta taaaatcctg ttacttgtat tttatgttcc tggaaatact
      181 tcccgaatga acaacctacc ttgccggctc caataaaacc aacgttcata attatcacct
      241 ccataaagaa gtgagactac aagtcccatt cttacgatat gagcttaaaa ataaaaatta
      301 aatcaaatat acagtatgtt tttttcaaat atcatccata gttaatttat cataatatcc
      361 catattttaa aataaaagat agcgtaaaat attttgtgta aatagaaatt tagggtagaa
      421 aaaaaagtcc attctgtaaa attaaagtta ccacacagaa attttatagg aggactttct
      481 catgactaaa tttactatag aaattatgca aacactactc aataaagggg atttagatga
      541 attatttcag gatcatttag agcgagcgat taattcactt ctacaagcag agctaactgc
      601 ttttttagat tacgaaaaat atgatcgaaa tggattcaat tcaggaaatt cccgtaacgg
      661 gaattattca cgtacattta aaacagaata tggcgagttg aatttaacta ttcctagaga
      721 tcggaatggc gaattttctc aacaaacact accagcatat aagcgaacca atgattcatt
      781 agaaacgacc attatccaat tatttcaaaa aggcattact atggctgaaa tctccaaatt
      841 aattgaaaaa atgtatggtc accactatac gccacaaaca atctccaaca tgagtaaatt
      901 ggtggctgaa gatgttttag cttttaaaga aagaacgtta gaagccaatt attccgttat
      961 atttatggat gcgacgcata tccctgtgaa gcgacagact gtttcgaagg aagcagtgta
     1021 tattacgatt ggtattcgtt tggatggaac caaagaagtt cttgggttta ctattgcccc
     1081 aactgagtct gcttatattt ggaaagaagt tcttcaagat cttagaaaac gtgggttaga
     1141 agaagtttta ttagtagtga cagacggatt aagctgtatt gaagaaagta tccatagtgt
     1201 gtatccgaat gcccaatttc aacaatgttg tgtgcatgta tctagaaata tcgctcataa
     1261 agttcgtgtt cgaggtcgaa aagaaatttg tgaggatttc aaattggttt accaagcgaa
     1321 ttcaaaagaa gagg
//
LOCUS       DACDYM010000191         1334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDYM010000191 DACDYM010000000
VERSION     DACDYM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.206"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1334
                     /locus_tag="IWM59_002790"
     rRNA            <1..>1334
                     /locus_tag="IWM59_002790"
                     /product="23S ribosomal RNA"
BASE COUNT          357 a          293 c          406 g          278 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggac
//
LOCUS       DACDYM010000192         1328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDYM010000192 DACDYM010000000
VERSION     DACDYM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.207"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            527..1060
                     /locus_tag="IWM59_002791"
     CDS             527..1060
                     /locus_tag="IWM59_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9980762.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          429 a          244 c          247 g          408 t
ORIGIN      
        1 cagatgcaag cttttgaaca ccgtttatta agaggttgtg aaaaagctgc actgcatcaa
       61 gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt ggacttatta
      121 ccgcagatgc aagcttttga acaccgttta ttgagaagtt gtgaaatcag cgtcctgacc
      181 tgagtattag agaaacaaat ggcaaaatag ttcctcatat tttgctattt tttgagctaa
      241 taccgagggt cagctgattg aacaccgttt agtggagaag ttgtgaaaaa gtcgcctact
      301 ttcgagtcgt aagaaatttt tttgaaaatg gttcctcaca actttgcata aatttgactt
      361 aataccgaga agtcatgatc cccatctaac tccaaatcac aaggaatgag ttcaaaaact
      421 agcttttctc aacaaatatt acataaaaaa ttctatgttt tttgttaaat ttatttgctt
      481 ttttcaaaag tatgttacag taatttccca aggaggtttt acagaaatga attttgttgc
      541 catggatttt gaaactgcca atcatcagcc ttatagtgct tgttctcttg cgctagtcat
      601 ggtaaaaaat agccagatcg ttgatgaatt ctatacattg atacagcctg agactccttt
      661 tttctggcga aatgtccaaa ttcacggaat ccatcaagaa gacgtacgca atgcgccgaa
      721 attcccagaa gtctggcaaa ctatccaacc atattttcaa aaaaatcgtt tagtcgttgc
      781 acataatgct gcttttgata caaaggtctt agcgggatgc ttagattatt atcatcttga
      841 acagcctaat tatctttctt tatgtacagt gaaaagtagc cgtcgcctgt ttcctgaatt
      901 tccaaatcat cgattgaaca ctgtgtgcga aaatttgaac atccaattag atcatcacca
      961 tgatgcattg gaagatagtc gtgcatgtgc ggaaatcctg ctttatcaag aaaaaaaatt
     1021 cgggacagat ccattgaaaa aacttgtgac tataaagtaa aagaatactt atgaaacaca
     1081 caaagaggtc gtgagcctgc cgtttcactc cgagtcacaa ggagcaattt caaaaaatag
     1141 cttctcatgt ttattaaata ttgtgactgg tgaccgagga gctggcagga gaacaccgtt
     1201 tattggagag gttgtgaaaa agctgttctg catcaagtaa taagaacagc caaacaaaaa
     1261 tagcttctca tatttttcat ttggttggac ttattaccgc agatgcaagc ttttggacac
     1321 cgtttaca
//
LOCUS       DACDYM010000193         1312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDYM010000193 DACDYM010000000
VERSION     DACDYM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.208"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(156..1297)
                     /locus_tag="IWM59_002792"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="IWM59_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9980763.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          323 a          263 c          278 g          448 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc tt
//
LOCUS       DACDYM010000194         1302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDYM010000194 DACDYM010000000
VERSION     DACDYM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.209"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            52..>1302
                     /locus_tag="IWM59_002793"
     CDS             52..>1302
                     /locus_tag="IWM59_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9980764.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACDYM010000195         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDYM010000195 DACDYM010000000
VERSION     DACDYM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.210"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IWM59_002794"
     CDS             <1..1244
                     /locus_tag="IWM59_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9980765.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACDYM010000196         1276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDYM010000196 DACDYM010000000
VERSION     DACDYM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.211"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            35..532
                     /locus_tag="IWM59_002795"
     CDS             35..532
                     /locus_tag="IWM59_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9980766.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(515..1252)
                     /locus_tag="IWM59_002796"
     CDS             complement(515..1252)
                     /locus_tag="IWM59_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980767.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAIYQKG
                     KRIMVPLPVQKNVLSIKSEDVFLDFFF"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt ggactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac cttttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt aattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgacc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat ttcgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DACDYM010000197         1265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDYM010000197 DACDYM010000000
VERSION     DACDYM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.212"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1265
                     /locus_tag="IWM59_002797"
     CDS             <1..>1265
                     /locus_tag="IWM59_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9980768.1"
                     /translation="QSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLG
                     KYYFVEVPSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNY
                     KAPVIDKTVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSV
                     FKWGDTAGKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVS
                     DTVANLHGQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTY
                     GAKFLKVDSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVAN
                     AAILKSDKEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIK
                     NNQKASVPMTGSNGFQSYVLISCLLLGAG"
BASE COUNT          476 a          205 c          269 g          315 t
ORIGIN      
        1 ccaaagcaaa caaacccttt taaaaggggc aaaatttgag ttatacaagg gcgaacctgg
       61 aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa ggaaagctga ctgcgactga
      121 tttgacgtta ggaaaatact attttgtgga agttccttcg gaagttgtag tgggatcaga
      181 tcaagaacca acagcagatc aatatttatt aggcgcagac gctcgcaacg atgcgcacaa
      241 taaattaacc tttgaaatta cgaatgatgg cgtgacaagt gacttaaaag cttcatacgt
      301 aaactataaa gcaccagtga tcgataaaac agtaacaaat ggaacaggac aagaacattc
      361 tttccaaatt ggtgacgcag taaattatca aggaacgatt catattccaa cagacattgc
      421 aggtggcgca gacggtatca cagtaaacgg tgtgaaaagc gaaacttctc cttacagtgt
      481 atttaaatgg ggagatacag caggtaaagg actttcttat gttgctgaaa aagcagatat
      541 taaagtaaca aataatgatg gttcagtcgt attaaaagaa aatactgact ataaaatcga
      601 aaattctgat agtggttttg tgatcgattt tatcgtaaat aatggacaag taagtgatac
      661 agttgcgaat ttacatggtc aagatctaaa aatgacttat aacatgtttg tgaatgatag
      721 tgctgaaatc gcaaatccat taacaaatag cgtggatttc gtttataaca ataatccatt
      781 taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt gtgacttatg gcgcaaaatt
      841 cttaaaagtc gatagcggcc tatttggaac aggtatcaaa gcaacaccgc ttgaaggcgc
      901 agaatttgca gctaagaatg cagaaggtaa gtactacggt ggacttactg atacagataa
      961 agatggtgtc aaagaagcag tttgggtaga cgatgtagca aatgcagcaa tcctaaaatc
     1021 agataaagag ggtcattttg aaatcactgg tttaactgaa ggagattact cactggaaga
     1081 aacaaaagct cctgaaaact atcaaaaatt aacaaaagag atctctttca aagtagacaa
     1141 agattcatac aaagaagaaa accgaatcac gatcaaaaat aatcaaaaag cttccgtacc
     1201 aatgactggt agcaacggtt tccaatctta cgtattgatc tcttgcttgt tgctaggagc
     1261 agggg
//
LOCUS       DACDYM010000198         1265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDYM010000198 DACDYM010000000
VERSION     DACDYM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.213"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1265
                     /locus_tag="IWM59_002798"
     CDS             <1..>1265
                     /locus_tag="IWM59_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9980769.1"
                     /translation="QSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLG
                     KYYFVEVPSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNY
                     KAPVIDKTVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSV
                     FKWGDTAGQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVS
                     DTVASLHGQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTY
                     GAKFLKVDSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVAN
                     AAILKSDKEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVK
                     NNQKAAIPLTGSNGFQTYVLISCLLLGAG"
BASE COUNT          476 a          203 c          272 g          314 t
ORIGIN      
        1 ccaaagcaaa caaacccttt taaaaggggc aaaatttgag ttatacaagg gcgaacctgg
       61 aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa ggaaagctga ctgcgactga
      121 tttgacttta ggaaaatact attttgtgga agttccttcg gaagttgtag tgggatcaga
      181 tcaagaacca acagcagatc aatatttatt aggcgcagac gctcgcaacg atgcgcataa
      241 taaattaacc tttgaaatta ccaatgacgg cgtgacaagt gacttaaaag cttcctacgt
      301 gaactataaa gcaccagtga tcgataaaac agtgacaaat ggaacaggac aagaacattc
      361 tttccaaatt ggtgacgcag tgaattatca aggaacgatt catatcccta cagatattgc
      421 aggtggaaca gatgggatta cagtaaatgg tgtgaagagt gaaacttctc cttacagcgt
      481 atttaagtgg ggagatacag caggacaagg actttcttat gtcgctgcta aagcaaatat
      541 caaagtaaca aataaagatg gatctgtggt attaaaagaa aatacagatt ataaaatcca
      601 aaattcagaa aatggctttg tgatcgattt tatcgtgaat aatggccaag taagtgatac
      661 tgtagcaagt ttacacggac aagatcttca aatgacttat aacatgtatg taaatgacag
      721 cgcagctgtt gccaatccat taacaaatag cgtggatttc gtttataaca acaatccatt
      781 taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt gtgacttatg gtgcaaaatt
      841 cttaaaagtc gatagcggac tatttggaac aggaatcaaa gcaacaccgc ttgaaggcgc
      901 agaatttgct gctaaaaacg cagaaggaaa atattatggt ggattagttg atacggataa
      961 agacggtgtc aaagaagcag tatgggtaga cgatgtagca aatgcagcga tcctaaaatc
     1021 agataaagaa ggtcactttg aaatcactgg tttaactgaa ggtgaatatt cactggaaga
     1081 aacaaaagca ccaactggtt accaaaaatt aacagaagat attgcattta aagtaaacaa
     1141 aaactcattt aaagaggaaa atcgaatcac tgttaaaaat aatcaaaaag cagctattcc
     1201 attaacaggt agtaatggtt tccaaacgta tgtattgatc tcttgcttgt tgctaggagc
     1261 agggg
//
LOCUS       DACDYM010000199         1261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDYM010000199 DACDYM010000000
VERSION     DACDYM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.214"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            48..1238
                     /locus_tag="IWM59_002799"
     CDS             48..1238
                     /locus_tag="IWM59_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP9980770.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
BASE COUNT          389 a          260 c          283 g          329 t
ORIGIN      
        1 gtagaataga gttaccacaa cacattcaca gaagagagga cttccctatg aacgatttta
       61 ctacagaaat tctaaagact ctagcgaaca aaggcgattt gaatgaatta ttccgtgtcc
      121 atttggagaa agcagtcaat acgcttctca aaacggagtt aacggctttc ctagattacg
      181 aaaagtatga tcgcattggt tttaacacgg gtaattctcg taacggctcc tatgaccgta
      241 cggtcaagac cgagtatggg gaacttcatc tccagattcc gcgcgaccgc aatggtgagt
      301 tcaagcaaca gactgttcct gcttatagac ggacgaatga cacgttagag gagaccgtca
      361 ttcacctctt ccgaaaaggt attaccatgt cggaaatcgc agacttgatt gagaaaatgt
      421 atgggcatta ctacacgccc caaaccatgt ccaatataac aaaatcattt acagaagagg
      481 taacggcgtt taatgggcgg gagcttcatg accgttatgc tgctatttat atggacgcaa
      541 cgtatattcc gttaaagcgg aaaaccgtcg ccaaggaagc tattcatatc gcagttggca
      601 ttcgcccgga cggatcaaag gaagtattga gctatgcgat tgcaccgact gaatccatca
      661 cgatttggga ggaaatttta ttggaccttc aagagcgcgg tttgaaaaat gtcctcctgt
      721 tcatcacgga tggcttaaag gggatggtag gagcgattag tcggttctat cccaaagctc
      781 gttttcaaca ttgttgtgta cacgtttccc gtaatatcag tcataaagtg cgtgtcgatg
      841 atcgtaagga agtctgtgat gattttaaaa tggtgtatca agcctcatct aaagaggtgg
      901 cgttggaagc acgtggtact tttgcggaaa aatggaaaac cagctatcca aaagtggttg
      961 aatcgattct ttcgaacgat cacttgctca ctttctatga tttccccttg gccatacgca
     1021 agagtattta ttctacgaac ttgattgaat cctttaataa acaaatcaag aaatacagcc
     1081 accgcaagga acagttccaa aatgaagagt cgatggaacg tttcttagtc tcgtcttttg
     1141 atacttacaa ccaaaaattc ctaggtcgca gtcataaagg tttccaacag gccgaaggcg
     1201 agctcgaaca aatgctaagc caactgattg agaattagaa acaatctgca gtagggaaga
     1261 a
//
LOCUS       DACDYM010000200         1242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDYM010000200 DACDYM010000000
VERSION     DACDYM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.215"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..525
                     /locus_tag="IWM59_002800"
     CDS             <1..525
                     /locus_tag="IWM59_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9980771.1"
                     /translation="KQYRTKGIAYFSKEDFHELLDIPKSYRESDINKKIIKPIRQELT
                     AIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQI
                     YSEKDPTSLQEKIKEQKRKLELLQQLEELERKQNNLNKENKEINEQIPDNNSDIPPEL
                     KKGLLDNLQNLFKE"
     gene            535..1032
                     /locus_tag="IWM59_002801"
     CDS             535..1032
                     /locus_tag="IWM59_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9980772.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
BASE COUNT          531 a          215 c          192 g          304 t
ORIGIN      
        1 aaacaataca gaaccaaagg aattgcctat ttttcaaaag aagactttca tgaattactt
       61 gatatcccta aaagttatag agaaagtgat ataaataaaa aaattataaa gcctattcgc
      121 caagaactaa ccgcaatctt caaaggtctg acaatcaaaa agaaatacgg aaaaggaaga
      181 ggaaaacctg tcattggcta tcaattcacg tttaaacctg aaataaaaaa agcagacgac
      241 tttaataaac acgcaccaac aaaagaagaa caaaataaag ctttagaact agttaatcaa
      301 caaatttact ctgaaaaaga ccccacttca ttacaagaaa aaatcaaaga acagaaacga
      361 aaattagaac ttttacaaca attagaagag ctagaaagaa aacaaaataa tctgaataaa
      421 gaaaataagg agattaacga acaaatacca gataacaatt ccgatattcc cccagaatta
      481 aaaaaaggct tactagataa tcttcaaaac ttatttaaag aataaggggt ttttatgaca
      541 gataaaacta tcaaagaatt agcagatatt ctaggtgttt caaaagataa agtgaagtat
      601 caactgagga aattacctag taattttacc tacaaaaaag gaaatattat ctacctgaaa
      661 aaagaagcag taacaacaat tacaaaggtt ttatcaggta atgagccggg taacttacct
      721 agtgaattac ctgattcatt acctaccgat tacctactca aggagattga aactaagaat
      781 aatcatattg aagaaaaaga taggcaaatt gaaaaacttc aactagcttt agaaacacaa
      841 caaaaattac tcgaccagca acaacaactc accctgcaag ccaaccaaca aatcgagaaa
      901 ctgcaggaac aactacagtt gacttatacg gaagaatctt cggaaaataa atcaacgact
      961 ctttctgaag aaacagaatc gatagaaaaa caatcaaaga cggagaaaaa atggtggcaa
     1021 ttctggcaat aaaaccagca atttaaatta ataaaaattg ctggttttat tcttcttttt
     1081 gcttttgcga gcagagcaac atgggcccat gttgcgatat tttggtggcg ccccaatccc
     1141 cgttttttcg ttgaatctta ctcggactgc actcgtttta cagctgattt ttctgaattt
     1201 tgaaagagca taaggaaacg tggtatcact ttcttacgaa ag
//
LOCUS       DACDYM010000201         1189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDYM010000201 DACDYM010000000
VERSION     DACDYM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.217"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            142..399
                     /locus_tag="IWM59_002802"
     CDS             142..399
                     /locus_tag="IWM59_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980773.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(631..1044)
                     /locus_tag="IWM59_002803"
     CDS             complement(631..1044)
                     /locus_tag="IWM59_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP9980774.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          437 t
ORIGIN      
        1 tttctgtcca cgttcatttt actccaaagt tttcctcatt tcaaaagaaa atcacagaat
       61 acacgaagaa gcgtttctat ttgtataaac tatatcaaag ttctatactt aaattatcaa
      121 ataaaaagga cgtgaagata aatgtcaaat ttagaaaaca aagaagaaaa agttgtaaac
      181 aaaattgttt ctgttgtcaa taaactggat aaagagttag acgaacttga tacgttatct
      241 gaaaatccag aaaaaaaaca taacctaaaa aaatggttag ttgaacgaaa ggctattcat
      301 gaaattaaaa aagttcttca tgaagctgat aaatatgaaa aatatgatga aaaagaactt
      361 gataaagaat ttaaagaaat caatgactta ttactataat tgatttatac gactcatttt
      421 tgagtcgtca gactgcaaac aacactctaa atttaggagt ttgtctacag tctgaataaa
      481 ttttaaattt attgtccata atgttattat gaatttatag cattaagaaa agagataaga
      541 ctttacattt ttttaggaat gtaaagtctt atctcttttc ttaattgtac aattagtatt
      601 atctgcacta ctttctaatt aaataaattt ttagttatta acttgattaa aatcagagat
      661 aatatcattt aattcagttt gaagggttgt aacgattaaa tggaaatttt cttcatctgt
      721 tttattatat ggggatgttt tcgttatata taacaacttt tctttttcaa ttttcattaa
      781 taactctgtc gaagtctgta ctttatcttg ataattaaat gcagacatac cactttgtat
      841 aattgtaatt attgctgaag caattgaaac aatcaatagt aaagacctgt gttcggaagc
      901 ctgatcaatt aatattggga tactagctga taagactatt tttataatat ttccgatacc
      961 aattactcta ttataaaatc gaatttcttt ttttaatcta tcgattgtag aatcaataga
     1021 ttcgataaac gctttttgat ccatcatttt ctctccaatc attttattaa aatcattaac
     1081 aaaaatatta taataggatt taagtaagtt ctaactaatc acatataaaa ataaaaaaac
     1141 atttgtattt tatcaaagtt ttattatact tgtcaatttg tgtacatcc
//
LOCUS       DACDYM010000202         1156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDYM010000202 DACDYM010000000
VERSION     DACDYM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.218"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWM59_002804"
     CDS             <1..378
                     /locus_tag="IWM59_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980775.1"
                     /translation="QTPVNNQANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHI
                     NQSSQADSVATTASIKEEAQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKA
                     KEGSSDSTVQSNTSESSESNEVK"
     gene            378..>1156
                     /locus_tag="IWM59_002805"
     CDS             378..>1156
                     /locus_tag="IWM59_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAP9980776.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSM"
BASE COUNT          411 a          204 c          229 g          312 t
ORIGIN      
        1 caaacgccag tgaacaacca agcaaattta actccttcac cccagcgttc ctttaacgga
       61 tggatggtgg gatgtattgc aagtttaagc ttgcttctag gtggaggaac agtatttgct
      121 tttaatcata tcaatcaatc ttcacaggct gattctgtag ccacaacggc gtcaatcaag
      181 gaagaagccc aggaacaagc aagaaaagag tatgaacaac aattagaaga attattgaat
      241 cgtaccaatc agtcaacaac agagtcaagc atggaaaata gcaaagagag tgattctagt
      301 aaggctaaag aaggatcaag cgattcaact gtacaatcaa acacttccga aagttcagaa
      361 tcaaatgagg tgaaataatg cgtgttagag aaacaacgaa gcgaaaagac aaacgattca
      421 atttagaaaa gaataggtat ctttctcatt tctatcgcta tactccctca ggagaagagc
      481 taccagaaaa acatgacaaa cgcatttcga aaaagatttt ttatagtgga cttgtatacc
      541 tccctttaga gtatttagta acaagagtac cagaagtttt caaccgattg gaaattgtta
      601 agggaagctt acaaggaata aatctgtttc aattacagga aatgccacag gtctttcatt
      661 ttgattcagc tggtttttgg aatccattta ctcctacaac tagtttgggt acacgtttag
      721 caatttcgac agtttgtgtg gttccagctg tacttatggg attgaaaatg gacatgtggt
      781 ttcgtcctct agcagatgga caaaaagggg ataatcgatt aatgacctta aaagaaatcc
      841 aacagattta tccgcaaata cctgaaacaa aaactacttt tccaggttat ggaggtattc
      901 caatcacaca ttacaaaaaa tattggtaca ttcaaacaga tactgttaat acgtgtattg
      961 ttggtacctc tcgatcaggg aaaggtcaga ttgaagtgtt agccaccatt gacaatttat
     1021 ctcgtgcaga aaaacaaagt tcaatggtcg ttaatgatcc aaaaacagaa ttatatgttg
     1081 gtagtaaaga tgaactagaa gctagagggt atgatgtata tgcctttaat atcaccgatc
     1141 cactgcaatc aatgag
//
LOCUS       DACDYM010000203         1147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDYM010000203 DACDYM010000000
VERSION     DACDYM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.219"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(113..197)
                     /locus_tag="IWM59_002806"
     tRNA            complement(113..197)
                     /locus_tag="IWM59_002806"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(161..163),aa:Leu,seq:caa)
     gene            complement(218..288)
                     /locus_tag="IWM59_002807"
     tRNA            complement(218..288)
                     /locus_tag="IWM59_002807"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(254..256),aa:Cys,seq:gca)
     gene            complement(334..405)
                     /locus_tag="IWM59_002808"
     tRNA            complement(334..405)
                     /locus_tag="IWM59_002808"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(371..373),aa:Gln,seq:ttg)
     gene            complement(417..489)
                     /locus_tag="IWM59_002809"
     tRNA            complement(417..489)
                     /locus_tag="IWM59_002809"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(454..456),aa:His,seq:gtg)
     gene            complement(492..565)
                     /locus_tag="IWM59_002810"
     tRNA            complement(492..565)
                     /locus_tag="IWM59_002810"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(531..533),aa:Trp,seq:cca)
     gene            complement(572..652)
                     /locus_tag="IWM59_002811"
     tRNA            complement(572..652)
                     /locus_tag="IWM59_002811"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(616..618),aa:Tyr,seq:gta)
     gene            complement(654..729)
                     /locus_tag="IWM59_002812"
     tRNA            complement(654..729)
                     /locus_tag="IWM59_002812"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(694..696),aa:Phe,seq:gaa)
     gene            complement(765..837)
                     /locus_tag="IWM59_002813"
     tRNA            complement(765..837)
                     /locus_tag="IWM59_002813"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(802..804),aa:Asp,seq:gtc)
     gene            complement(847..919)
                     /locus_tag="IWM59_002814"
     tRNA            complement(847..919)
                     /locus_tag="IWM59_002814"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(884..886),aa:Val,seq:tac)
     gene            complement(935..1006)
                     /locus_tag="IWM59_002815"
     tRNA            complement(935..1006)
                     /locus_tag="IWM59_002815"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(971..973),aa:Glu,seq:ttc)
     gene            complement(1021..1110)
                     /locus_tag="IWM59_002816"
     tRNA            complement(1021..1110)
                     /locus_tag="IWM59_002816"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1074..1076),aa:Ser,seq:gga)
BASE COUNT          327 a          289 c          256 g          275 t
ORIGIN      
        1 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
       61 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
      121 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
      181 aattccgcca cgccggcaag ttgatttatt agtactaagg cggtaaccgg atttgaaccg
      241 gtgatgaagg ttttgcagac ctctgcctta ccacttggct ataccgccat tatactatta
      301 tcaatctata aaataaagaa taaattatag aaactggggt agctggattc gaaccaacgc
      361 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaaaagggcg
      421 aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc
      481 cacattcgcc atggcagggg cagcaggaat tgaacccaca ctaacggttt tggagaccgc
      541 tgttctacct ttaaactatg cccctataaa atggaggaga gtggattcga accactgaac
      601 cctaaggaac ggatttacag tccgtcgcgt ttagccactt cgctactcct ccatggtggc
      661 gcgggacaga atcgaactgc cgacacacgg agcttcaatc cgttgctcta ccaactgagc
      721 taccgcgcca tattttaaaa ttattaattg aataacaaga acgacggttc cgacgggaat
      781 cgaacccgcg atctcctgcg tgacaggcag gcatgttaac ccctacacca cggaaccagt
      841 tcattatgga ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc agatgctctc
      901 ccagctgagc taaacctcca acattcaatt aaaatgaccc gtacgggatt cgaacccgtg
      961 ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa cgggccaggt aaatttgcta
     1021 cggagagtaa gggattcgaa cccttgagac agtttggcac catctacatg atttccaatc
     1081 atgctccttc ggcctctcgg acaactctcc agagtgaaag aagctcaata ctctttctca
     1141 aaacgag
//
LOCUS       DACDYM010000204         1115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDYM010000204 DACDYM010000000
VERSION     DACDYM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.220"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..898)
                     /gene="vanY"
                     /locus_tag="IWM59_002817"
     CDS             complement(<1..898)
                     /gene="vanY"
                     /locus_tag="IWM59_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9980777.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          400 t
ORIGIN      
        1 tagtatgtgt tgatccggga aacactgtca caattacacc gtctatattg tttcctgata
       61 tctcataacg aagattagtc ggcacatgaa tggtggtatt tttagtaaca ggataataaa
      121 agatctcata tttttcccca tttacactaa cagaaatggt tttttcttct tttaggtaat
      181 ccatatattc ctcgagaacg aaattctttt ctttcataat cgcactatgt ggtaaaccaa
      241 catagcgaat atgccatggt tcatattgaa ttcctgttaa ctctgttttg tcctctggat
      301 aacgtaaaat gaacccgtat ttccaagcat tttcttctat ccactttcct tcaggggctc
      361 gttccatttt cgtcaagctt gatcctacat ctagtgataa acctgaatta tgctcactat
      421 aacctgctgg taaggcatac tcagccccca tttcttggta aagcacactt tgctcatcaa
      481 agtctcgata gccactatta ataataaaat gactaacgcc accctttaca gcatcattga
      541 ccatctctga aaatttttgt gctatttctt ttgacatata aatattacta tcaagcaacc
      601 cgtatccatt tattaattcg tcatgtttag ataaattcac gatatctgac ttcacacttt
      661 cttggcgaac aggatattta ctattgatta atagcagatt tccttgataa acctgttctt
      721 ctgtaattgt tttctcttgg gtattttcag aagtcccact attttctaaa tgttctttgg
      781 gattttgatc ataattttga aattcgactt tttcctgaga aaacagtgct tcattaacgt
      841 agtcataacc taagtatatt aagaataaca ataacaataa aaaaaacaac ttcttcattt
      901 tcagtctcct ccttaactat atagttggaa gtatgaaata tttctagaat cttcccctta
      961 aacggcacaa tctcgtttga gattgtgccg ttttgccccg taaattacag tcaattattt
     1021 cgccctactg gatttactat tatcaccaat gtagatgaat tttcataagt gatacccatg
     1081 ctgaatttaa cgaggctgaa ctgcctgttg acaat
//
LOCUS       DACDYM010000205         1110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDYM010000205 DACDYM010000000
VERSION     DACDYM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.221"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..567)
                     /locus_tag="IWM59_002818"
     CDS             complement(<1..567)
                     /locus_tag="IWM59_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9980778.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRR"
BASE COUNT          353 a          217 c          138 g          402 t
ORIGIN      
        1 tcttctaaaa tcttcaactt tgactaccca ataaccattt cgccactctt tatttctgta
       61 ttgcatgaga tgacgataca attcttttgt gtagctactt cttagttttg taaaattatc
      121 aagttcaaaa cgagtaaagt ttttgccaat tgagtttaag ataaattcaa attcctcatt
      181 tgccttaatt gtaactgttt ccttctgttt atctatccgg aatttttgaa aaagccacag
      241 ctgttcatag acgtctgaat cttcaaaacg gtaggttaaa gtacctagtt tgtcactcaa
      301 agcttttaac acagaataaa aactgtcctt ttctcttctg tcgtatttga ccaaataacg
      361 aaagaggttg aaatcaaaag ttacttctgc tgttccttga cgtttcattt ttgataaaat
      421 agcccaaaac acatccattt caacaggctt aaatcctcgc aaagctgttg tattcagacc
      481 attgttataa cgcacaattg catttgtttg ttctgatact aaatctacat cactatcaat
      541 aatcttttcg gtaacttctt gagacattga ttactctcca atcttctttt tgcgctcctt
      601 taatcatgtt aatctggtac taattaaagg aaacaatttt ttctgcttgt tacctaaatt
      661 aaatcacaac taaaggtaac agtcaacaaa gatttgttac taatctaatt ttctattttt
      721 tcgccttcca aatcaagaaa accgaaggta acgtcttatc ctagtgtgat acctcaaatg
      781 tccacttttg ttacctttgc tgtccaaaag tgttaccctt actgtccact tttgttacct
      841 ttactgtcca aaagtgttac ctttataccc ctttaatact tactctccca agagattaag
      901 cctcccctaa cagatatatt aacagatatt taacagatat attaacagat atttaataaa
      961 ggaggattac tcctctctta tttcaaaatc ttaaaaataa aaaatcttaa aaaaaactaa
     1021 cactatctag gagtttattt tgcttgtgta actttgaata attgaaaaaa aggaaaagga
     1081 ggaagcttca gcttcccctt ttatttatat
//
LOCUS       DACDYM010000206         1089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDYM010000206 DACDYM010000000
VERSION     DACDYM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.222"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..978
                     /locus_tag="IWM59_002819"
     CDS             4..978
                     /locus_tag="IWM59_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9980779.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          382 a          135 c          187 g          385 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaaactaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaa
//
LOCUS       DACDYM010000207         1070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDYM010000207 DACDYM010000000
VERSION     DACDYM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.223"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..>1070
                     /locus_tag="IWM59_002820"
     CDS             25..>1070
                     /locus_tag="IWM59_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAP9980780.1"
                     /translation="MDTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLE
                     QADQTGDYSKLTPNNLVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQ
                     YGATKIGSDKAKGNILSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHES
                     LLNQRIVVEFYHKGKKFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAW
                     YEFSQKVLKDEEGKIVYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPM
                     AIFMLTPDYDPSNHIIVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMD
                     NMEGIMTVTAGRNM"
BASE COUNT          382 a          174 c          209 g          305 t
ORIGIN      
        1 gtgaaatact ggaagcgagg agacatagac accgcaacac agttaaccaa tacttttacg
       61 aatacgattt attatgatgc aaatgcttca gacaataaat actttaacga aaatgcgcag
      121 aaagctgtca atgcgcttat ttttgcgtta ttggaacaag cggatcaaac aggggattac
      181 tccaaattaa caccaaataa tctcgttgaa ctactttcag aaattggcgg ttttaattat
      241 tatccagatc ctgagaatga attcaagcag ctcaatgcct tagatgagtt catgaatcag
      301 ttaccccctg gaaatatagc aaaaaaacaa tatggggcaa caaaaattgg atctgataaa
      361 gcgaaaggga atatcttgtc tacggcaatt acaggattga atccatttac cctaacgaaa
      421 attgcgaaga tgacttctca atcgaccatt gattacaagt ccattggttt tccgaagtat
      481 ttggatctca aattacatga aagcctatta aatcaacgca ttgtcgtgga attttatcat
      541 aagggaaaga aatttcacga ggaacgagtc aaagttggct atcgtggttt ttgtgaaatc
      601 aactttgatt gtcatttaga gcagggagat catgtttgct taaaatacaa tcatcaagga
      661 aaacaatatg ttgcttggta tgagttttct caaaaagtat tgaaagacga agagggaaaa
      721 atcgtttatc gaaaaaaacg aggagaaact catttgccag aactagaaaa agaagccgtt
      781 ctaacattag atgagaagaa aagtaaccta ttagtagagt ctattcggat gcactatacc
      841 gataaaccaa tggctatttt tatgttaacg cctgattatg atccatctaa ccatatcatt
      901 gttagcatct ttcttagtca gttgtataaa gaattatcta cgcaatgtgt taagactaaa
      961 ggagataaat gtcaccgtag gattcatttt attttagatg aatttggaaa tatgccaccg
     1021 atggacaaca tggaaggaat catgacggtt acggcaggtc gtaatatgtt
//
LOCUS       DACDYM010000208         1058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDYM010000208 DACDYM010000000
VERSION     DACDYM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.224"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWM59_002821"
     CDS             complement(36..992)
                     /locus_tag="IWM59_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980781.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACDYM010000209         1037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDYM010000209 DACDYM010000000
VERSION     DACDYM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.225"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..193
                     /locus_tag="IWM59_002822"
     CDS             <1..193
                     /locus_tag="IWM59_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9980782.1"
                     /translation="TNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLN
                     RSHKGFQQVTDTLVSMFTE"
     gene            246..1028
                     /locus_tag="IWM59_002823"
                     /pseudo
     CDS             246..1028
                     /locus_tag="IWM59_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          436 a          151 c          137 g          313 t
ORIGIN      
        1 aaccaatcta atcgagtctt tcaataagca aattaaaaga tacagccgta gaaaagagca
       61 gtttcaaaat gaagaatcac tagaacgctt tctagtcagc atttttgata catacaatca
      121 aaaatttcta aacagaagcc ataaaggttt tcaacaggta accgatacat tagtttcaat
      181 gtttactgag taactaatta ttttgcagga ggacaattta tttacacaaa attattgacg
      241 ctccccaaga tatgctagat tactactatt ttgaaaaaaa tttatcatat aattatgtaa
      301 tacatttaaa aacttttaca ggaatgattt ttaaattcgc caaccgtcga tatggattaa
      361 aatataatcc cgtaaatggg gtttctttag caagaaaacc taagacaaac gaagaaataa
      421 aaaaagaaaa agatggttat ttatcaaaag aagaagttca aatgattgct agtaaacaaa
      481 aaagttcaca tcaacagtca cgttattctt taattactaa atttttgttt ttaactggac
      541 tacgttatgg tgaattgata tctttgactg aatctgatta tgatggtaat aaaattacgg
      601 tttctggtac ttatgattac gagttaaaaa ttaaaacaac aactaaaaat acaggttctt
      661 atagaaccat tgaattatca agtaatgcta aagaaatcat agatcaatta attgaagaaa
      721 acaaattaat taagaataat aaagataaat acattttcat ctctaaaaat ggtaacccga
      781 tatctattca agcatataat caatctttaa gagcagtatc caaagaatta aacatagata
      841 aaaaagtttc ttctcacatg ttaaggcatt cccacatttc tctacttaca gaattaggaa
      901 tcccattaaa agccattatg gatcgtgtag gacatgaaga ttcaaaaaca acattaaaga
      961 tttatactca tacaactaaa aatatgcaaa atcaactagt agaaaaacta ggaaaaatag
     1021 aaatttaaat tgcccct
//
LOCUS       DACDYM010000210         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDYM010000210 DACDYM010000000
VERSION     DACDYM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.226"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IWM59_002824"
     rRNA            complement(<1..>1035)
                     /locus_tag="IWM59_002824"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACDYM010000211         1024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDYM010000211 DACDYM010000000
VERSION     DACDYM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.227"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            281..973
                     /locus_tag="IWM59_002825"
     CDS             281..973
                     /locus_tag="IWM59_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9980783.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          441 a          145 c          170 g          268 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tata
//
LOCUS       DACDYM010000212          979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDYM010000212 DACDYM010000000
VERSION     DACDYM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.228"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..602)
                     /locus_tag="IWM59_002826"
     CDS             complement(<1..602)
                     /locus_tag="IWM59_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9980784.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT          320 a          173 c          187 g          299 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaattt gatcaaagtg gtagctgtta ttgaattcaa gaattgtttc tcgtatgaat
      481 gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa ttgattgtcg
      541 tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac aaataatttc
      601 atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac tgtgggattt
      661 cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat aaaagagcta
      721 gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca gctaatgagt
      781 tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac gctacaggag
      841 gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga gaaatcacac
      901 aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa tctagaaaca
      961 ttttgaataa aaactatta
//
LOCUS       DACDYM010000213          945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDYM010000213 DACDYM010000000
VERSION     DACDYM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.230"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            73..>945
                     /locus_tag="IWM59_002827"
     CDS             73..>945
                     /locus_tag="IWM59_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9980785.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          331 a          164 c          175 g          275 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataat
//
LOCUS       DACDYM010000214          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDYM010000214 DACDYM010000000
VERSION     DACDYM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.231"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..>943
                     /locus_tag="IWM59_002828"
     rRNA            2..>943
                     /locus_tag="IWM59_002828"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACDYM010000215          930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDYM010000215 DACDYM010000000
VERSION     DACDYM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.232"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            628..>930
                     /locus_tag="IWM59_002829"
     CDS             628..>930
                     /locus_tag="IWM59_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9980786.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVS"
BASE COUNT          316 a          119 c          216 g          279 t
ORIGIN      
        1 atcggctgaa ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat
       61 attgtcgaga cttctaaagg aacttagagt cactagaaaa ataaggagta gacttgatct
      121 atcaacaatt cccttcgggg agtgacgggg taggttttaa cgattggtaa cgtaaaaaat
      181 tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa atgaatttgt gtaaataata
      241 agcgcagagt aaacaagagg gaatttagaa gttagacaag ttagaatagc gtctagatta
      301 atcatgagca gctactaagg gatctagact ttgacgcaaa ctcatggaat aaggagtagg
      361 tcttctcgta atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat
      421 acttgcgagg acgttgatta ttggatcaat cgagcattta ccgctgtcta ctagggtttc
      481 tcgtttcgga cgccataaac gagtcgaaat tttttatgta tttttaagta aacgaaaaac
      541 gataaaatat gaacaagtcc tgaataaagt gaaaaaatta atataaagca tagagaaaat
      601 gaaatttacg taatggagga atgacaaatg aaaaattatg atccgaatat acgattgggc
      661 acacatacta ttaaggtttc tttccaacgt tgggattaca aaggatttgt gacgtttaga
      721 aggggcggaa attgtaaagg attagatgtt ttagcattag acgaggatga tttatacgat
      781 caaacattga ctgataatcc aattggattc ggcttgctac ctgaagatga tgaaggtaat
      841 gaatggttta aaatgacgct tatgaatgat aatggagacg aattatctgt tgaagatact
      901 tggtcatatt tgagtgatta tattgtcagc
//
LOCUS       DACDYM010000216          910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDYM010000216 DACDYM010000000
VERSION     DACDYM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.233"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..355
                     /locus_tag="IWM59_002830"
     CDS             <1..355
                     /locus_tag="IWM59_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980787.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="IWM59_002831"
                     /pseudo
     CDS             615..910
                     /locus_tag="IWM59_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DACDYM010000217          886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDYM010000217 DACDYM010000000
VERSION     DACDYM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.234"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            44..103
                     /gene="ermCL"
                     /locus_tag="IWM59_002832"
     CDS             44..103
                     /gene="ermCL"
                     /locus_tag="IWM59_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAP9980788.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            153..>886
                     /locus_tag="IWM59_002833"
     CDS             153..>886
                     /locus_tag="IWM59_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAP9980789.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          374 a          107 c          117 g          288 t
ORIGIN      
        1 tgtgctataa ttattctaat ttcataaaaa ggagaattaa tatatgggca tttttagtat
       61 ttttgtaatc aacacagttc attatcaacc aaacaaaaaa taagtggtta taatggattg
      121 ttaatataaa aacctcatag gagtgggtta taatgaacaa aaaaaatata aaagatagtc
      181 aaaactttat tacttcaaag catcatataa atgaaatttt gagaaatgta catttaaata
      241 caaatgataa tattattgag attggttcag ggaaaggtca tttcacgttt gaattagcta
      301 aaaggtgtaa ttatgtaacc gccattgaaa tagatcctaa attatgtagg ataactaaaa
      361 acaaacttat tgaatatgag aacttccagg ttatcaataa agatatttta caatttaagt
      421 ttcctaaaaa taagtcatat aagatatatg gaaatatacc ctacaatata agtacagata
      481 taattcgaaa aattgttttt gaaagcacag ctacagaaag ttatttaata gtggaatatg
      541 gatttgctaa aaggttgcta aatacaaatc gttcactagc actattttta atgacagaag
      601 ttgatatatc catattaagt aaaatcccta gagaatactt tcatccaaaa cctagagtta
      661 atagctcgtt aattgtatta aaaagacacc cttcaaaaat atcactcaaa gatagaaaac
      721 aatatgaaaa ttttgttatg aaatgggtta acaaagaata cataaaacta ttttccaaaa
      781 accaatttta tcaagcctta aaatatgcaa gaattgacga tttaaacaat attagctttg
      841 aacaattctt gtctcttttc aatagctata aattatttaa tagata
//
LOCUS       DACDYM010000218          845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDYM010000218 DACDYM010000000
VERSION     DACDYM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.235"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(39..251)
                     /locus_tag="IWM59_002834"
     CDS             complement(39..251)
                     /locus_tag="IWM59_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980790.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            438..>845
                     /locus_tag="IWM59_002835"
     CDS             438..>845
                     /locus_tag="IWM59_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980791.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKE"
BASE COUNT          291 a          108 c          151 g          295 t
ORIGIN      
        1 aataagaaaa gccttatata attttggctt ttcttatttt atttgttagt tgaagtatta
       61 atatcaacga tttcttcagc caatttattt aatttcatag catttatgaa tctcatgttg
      121 tctatactag agcgtttgtt ggcaaggtca cttactgtag ttaagggtac gccagattct
      181 atggatatag aacgaactga tttaccacta tcaaacagca accattcgat tttcttttta
      241 tcagcgtaca tttcaatgct ccttcttgtt ttttctttat tatagctctt ttttgtaatg
      301 aatcacaata tcaagagaaa aattttctat agaaaggtgg atttttatga gtattcgttc
      361 tccgtgtatc caaatgaggt ttgttgctac tttcgttatt tgatatacta aaaataaaca
      421 acgaaagaag gaataaaatg aagaaaaagc aactatggtt tgtctctatg tttgcaatat
      481 tttttattat tttaagtgga tgttcaagta ataagaaatc ggaagctaaa gaagataata
      541 gtttattgca accaggaact gtttggaaag aagaagtagg tggtcttatt cataatttaa
      601 aaattataga tgatactacc tgggaatatt ctgaaagtgt atggcatcca gatcctgtac
      661 aaataacagt taagaggcaa aaagactatg aaggtttaga aagatacaaa atagtagatt
      721 ctgcaggcat tagggaattt ataaacaaac ctgattcatt atttattgtt gttccctatg
      781 agaaggatgg cgtaaaaaaa ataatttttc ttggatctag caaggatgag aagcgaacga
      841 aagag
//
LOCUS       DACDYM010000219          820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDYM010000219 DACDYM010000000
VERSION     DACDYM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.236"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(602..675)
                     /locus_tag="IWM59_002836"
     tRNA            complement(602..675)
                     /locus_tag="IWM59_002836"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
BASE COUNT          283 a          137 c          142 g          258 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      721 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
      781 atatgcgata acatcactat tttatcgaat aatttttata
//
LOCUS       DACDYM010000220          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDYM010000220 DACDYM010000000
VERSION     DACDYM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.237"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWM59_002837"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWM59_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9980792.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACDYM010000221          763 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDYM010000221 DACDYM010000000
VERSION     DACDYM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.238"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(73..657)
                     /locus_tag="IWM59_002838"
     CDS             complement(73..657)
                     /locus_tag="IWM59_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9980793.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          240 a          165 c          108 g          250 t
ORIGIN      
        1 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
       61 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      121 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      181 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      241 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      301 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      361 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      421 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      481 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      541 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      601 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      661 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      721 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttt
//
LOCUS       DACDYM010000222          735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDYM010000222 DACDYM010000000
VERSION     DACDYM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.239"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          254 a           97 c          126 g          258 t
ORIGIN      
        1 attacaaaat aatagctatt ttaatagaat ttataagtta aaatattatg ataaaaatca
       61 caaacgtagc cttttacgtc ataagtgtta aaagaaataa ttaatttcaa atatagaaaa
      121 gggaacgtat gtttgcatac tccttttttt tgagttataa taatacgcga agagagataa
      181 tttcattatt taaacaagct aaaaatcttg atattacttc ttttcaggca atacgaatat
      241 tattcgctta cattttttgt ataaatttaa ttgtctgcta tgcttgtgat gaattgattt
      301 tcgttttgca cttttggatg ataaatttgg acaattgaaa atacaaaaaa aagaaaggcg
      361 gtatgaactc ggaaggctgg taaccttcgt cagcgttcat acacggcatt aagatttggc
      421 ccttaatgcg ttaacctttc agtgttacgg tattacccgc aatggattta gtataacaga
      481 tattcaaaag tgataccact ttttttggct gtgttaaggt taaaaatacg tgatattacg
      541 atgaatctat gcctaagcat aatgttataa aaatgtccgt gtggctcttt gccgtatgac
      601 gaacgctgaa attagaggga acgcatgttt ggatgcgttc cctctttttt gtttgtcatg
      661 aacgaaaatc aattctctat ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa
      721 agaaattggg tggtt
//
LOCUS       DACDYM010000223          713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDYM010000223 DACDYM010000000
VERSION     DACDYM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.240"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..448)
                     /locus_tag="IWM59_002839"
     CDS             complement(<1..448)
                     /locus_tag="IWM59_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9980794.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          216 a          123 c           95 g          279 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aac
//
LOCUS       DACDYM010000224          711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDYM010000224 DACDYM010000000
VERSION     DACDYM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.241"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..602
                     /locus_tag="IWM59_002840"
     CDS             <1..602
                     /locus_tag="IWM59_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9980795.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          181 g          190 t
ORIGIN      
        1 gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc aatgcggctg
       61 cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt tactatacga
      121 tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa gggtttagtc
      181 atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt gctggcagct
      241 gtccatttca tggcaactgt ttagaaggga tggccgctgg acccgccatc gaaaaacgct
      301 tagggaaaaa aggacaagag gttgcagaag atgatcctta ttgggaaatc gaagcctcct
      361 atattgctca atgtgcctac aatacaacct tgatgttttc tccagatgtg atcatttttg
      421 gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt ccacgacgca tttgcaaaat
      481 tggtcaatgg gtatgtcgaa acacctgagt taagcaaata tatcgtgacc ccagcattag
      541 aagacaacgc tggaacgctc ggttgtttgg cattggctcg agaagcggtc ttacattcat
      601 aaacgaaaaa aggctcttcg acatttagtg ttggtagaga aagatacaac tgaaaattta
      661 gtcataacca gttaggcaga gtgcttaact ggtttttgtt ttacttggaa g
//
LOCUS       DACDYM010000225          695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDYM010000225 DACDYM010000000
VERSION     DACDYM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.242"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(52..627)
                     /locus_tag="IWM59_002841"
     CDS             complement(52..627)
                     /locus_tag="IWM59_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9980796.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          228 a          151 c          125 g          191 t
ORIGIN      
        1 tgaaaaaaca gagccagatt agcattattt tgctaatcca gctctgttta ttcagcctat
       61 ttttcttttt gaatatatca agcgttgggg caacatagtg atgactaatg aaacaacgac
      121 gatagacaac aagcgatcaa ggtagtctgt acccgcctgt accaagatca cacttgttgt
      181 ctgacttaaa cctaacccat gtaatagctg aacgatcata ctggatcctg aggaggtgat
      241 cccgccaaat aactgaacgg taatgatcga agaaacgatc gttccaggta ccgacaaaat
      301 caaagcgagt aataacagtt tcaagctgtt tttatctttg ccgagatatc ttggcaaaat
      361 caatccagca agcaagcctg tgagaatttg gattggcgaa taatacagag aaaaaatatc
      421 tgtagtcact ccacttaaaa taccgctgga caatcctgtc agcattccag gtacaggccc
      481 aagcaatgca ccagcaaaaa tcgttccgat agaatcgaga tagattggca aacgaagcca
      541 taaagcaatc gttcctccaa tataattgat cccaatacac aaagccatca ctgtaagtac
      601 gcgagttgcc attttgtttt tattcatttt gttagcccat ctttaacctt tccaaatctt
      661 tcataatcgt ttcttcttgt gcatttaaaa gaacg
//
LOCUS       DACDYM010000226          684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDYM010000226 DACDYM010000000
VERSION     DACDYM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.243"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(164..517)
                     /gene="tnpB"
                     /locus_tag="IWM59_002842"
     CDS             complement(164..517)
                     /gene="tnpB"
                     /locus_tag="IWM59_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9980797.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          200 a          146 c          119 g          219 t
ORIGIN      
        1 tgtgtttgat tggttttatg ggcttcttga agcaactgaa gcaacgtatc tgtagtttcc
       61 aaaatttttc accaccttga atcgaactga ttttgttaca gaataccaca agaaacgcac
      121 aagaaatagg ggttcaacac attttttgtg ttaacccccc agcttaaaaa actccttttt
      181 ttcctggcaa aatagctcga ggttgatcga tacttaatcc ttctaacaac catcgaacct
      241 gttggttcgt taaggttcga acttcgtctt tcgtccgagg ccattttaac ttgccgtttt
      301 ctatccgctt ataacagaga agaaaaccat caccgtccca atagagaagt ttgaaacggt
      361 cttgacggtt cccgcaaaat aaaaaaacgg catcttcgta aagctcgagt tgatactctt
      421 gttgaataag actggcaaga ccatcaattc cttttcgcaa atctgttttt ccgcacacaa
      481 tataaatatg tttggtttga agtaaatcat aaagcattgg acaacagttc tttcaaaata
      541 gcttgaagaa tataattgtt aataccgtta tagatcaaaa cttcagaacc attctctctt
      601 ttgatgcgca tcgctaactt ggcagccgcg ggtttacctt tttctggtgc actcttttcg
      661 tttcctaatt ggacaggaac aatc
//
LOCUS       DACDYM010000227          678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDYM010000227 DACDYM010000000
VERSION     DACDYM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.244"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            35..277
                     /locus_tag="IWM59_002843"
     CDS             35..277
                     /locus_tag="IWM59_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980798.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            295..597
                     /locus_tag="IWM59_002844"
     CDS             295..597
                     /locus_tag="IWM59_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980799.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
BASE COUNT          232 a           97 c          139 g          210 t
ORIGIN      
        1 agttaaataa atacaaatta caaaagacta gtgagtgatt gctagtcttt ttatttatcc
       61 tgaaggagga atcacagtgg caaaagttaa aacaccattc gaaaaagccg cgtctaaaat
      121 cttagaacgc cgtggtgttt ctgaaataca gtggaaaaaa gaagttcttg aaaaagcaca
      181 atttgaccta ttacgaggaa aagataagga cttagaagaa tatatctatc aacgtgaacg
      241 tgaaaaactt gtattcgatg aaattattaa acattaaaag gagcgaataa tcttatgttt
      301 gaatcaatta caatgaattt tttaactcaa ccgaaagtag tagctgcagt tgatattttt
      361 agagcattac aaactgccgc aactagtttg aaaaataatc tgatgatttt tgctgctgtt
      421 gcggcagtgg cttccattgc tcttggaggt attttcttct tctttgggcg aagaggagct
      481 gaaaacggga agacacacgt aggttatgta ttgttggggg taggagttat tttagctgcc
      541 gcttctattg tagtttcaat gttctcaatg tttggacaaa ccccacaagg actataaaaa
      601 aaacaagtag gaggagtaaa aagtacgtgg ctacttttta ctcctttttt tatgaaagga
      661 gaatattcat gtttttta
//
LOCUS       DACDYM010000228          677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDYM010000228 DACDYM010000000
VERSION     DACDYM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.245"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          232 a           94 c          138 g          213 t
ORIGIN      
        1 acggtatgtg gttaaaaaag aaaaatagct aggagatttt tgagagatga ctggtaaatt
       61 taaaaatgat agaaatggag agtatgtatg agtaataaac aatttttatg gtcttctttt
      121 gcatttattg ttgttgatat ttttttccga tattttaata acgatatgat atttaactgg
      181 caaagaatta taatcgatta ctgtattttt tgtataatat attttttagt gaaaacattt
      241 ctaaaatacg caaaaaaata aaattgaaga tgttattaaa tcgcgattat ttacggagat
      301 ttatataaat aaaaaccccc tgacgaagtc gaagggggtt tttattttgg tttgaggttt
      361 gcatacctac cgaaaaggta gggcagcaaa tcgtcaaaca ggtatcagct aattgcacca
      421 taagggtgcg ctgatactcg gctcaaaaga gccgaaagag ccgtctgcaa gacccctcgg
      481 cgagcccaca ctttgcttgc aaagtatagt gggttatact ttacatggaa gtaattgccg
      541 gaaatagtga aaatgcctca catttgtgcc acctaaaaag gagcgattta catatgagtt
      601 atgcagttta cgggccaaga ttgtttaagc aaaataagat aagttaaggg atgcagttta
      661 tgcacccctt aacttat
//
LOCUS       DACDYM010000229          670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDYM010000229 DACDYM010000000
VERSION     DACDYM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.246"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            154..417
                     /locus_tag="IWM59_002845"
     CDS             154..417
                     /locus_tag="IWM59_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9980800.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="IWM59_002846"
     CDS             417..>670
                     /locus_tag="IWM59_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9980801.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DACDYM010000230          668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDYM010000230 DACDYM010000000
VERSION     DACDYM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.247"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>668
                     /locus_tag="IWM59_002847"
     CDS             <1..>668
                     /locus_tag="IWM59_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980802.1"
                     /translation="TFEERQQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQ
                     KVKERYEKKRDNAYSLCVEKINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTD
                     KLKISKELNEQLNKLYQTTTEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEK
                     QRQKEKEREQLEIRKREQDLAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQL
                     NYLA"
BASE COUNT          319 a           94 c          140 g          115 t
ORIGIN      
        1 acctttgaag aacgtcaaca agaaacagat caagaaatta atgagtatgc gacggaacag
       61 tcgtctaatt gggagaaagt aaaagaggaa ctaaataaag aagaacagcg agtgattgaa
      121 gaagaagtac aaaaagtcaa agagcggtac gaaaagaaac gcgacaatgc ttattccttg
      181 tgtgtagaaa agatcaacaa ttttactaag acgaatcaag aagcgttatc gaaagaaaaa
      241 gaaaacttgt taaaagaacg tattaaagaa cgaaaagaag agttctaccg cggtttacgt
      301 acagataagc taaagattag caaagaatta aatgaacaac tgaataagct atatcaaaca
      361 acgactgaag ttcttatgga aaaacacgaa cagatccagc aagaattaca agtacaaatt
      421 ccaaaatgga aaaaagagta tgaaattcaa caacaagaat tgaaagaaca agaagaaaaa
      481 caacggcaaa aagaaaaaga gcgtgaacaa ttagaaataa gaaaacggga acaggatctt
      541 gcggaacaaa aaatggcgat agaaaaacaa cggttagagc aagaaaagga aaaagaagca
      601 gctgctcgta aggaacgtca agaacaaatg gatacattgc gtcaagcaca gttgaattat
      661 ctagcaca
//
LOCUS       DACDYM010000231          668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDYM010000231 DACDYM010000000
VERSION     DACDYM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.248"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>668
                     /locus_tag="IWM59_002848"
     CDS             <1..>668
                     /locus_tag="IWM59_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980803.1"
                     /translation="TFEERQQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQ
                     KIKERYEKKRDNAYSLCVEKINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTD
                     KLRISKELNEQLNKLYQTTTEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEK
                     QRKKEKEREQLELKKREQDLAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQL
                     NYLT"
BASE COUNT          323 a           96 c          137 g          112 t
ORIGIN      
        1 acttttgaag aacgtcaaca agaaacagat caagaaatta atgagtatgc gacggaacag
       61 tcgtcgaatt gggagaaagc aaaagaggaa ctaaataaag aagaacagcg agtgattgaa
      121 gaagaagtac aaaaaattaa agagcggtac gaaaagaaac gtgacaatgc gtattccttg
      181 tgtgtagaaa agatcaacaa ttttactcag acgaatcaag aagcattatc gaaagaaaaa
      241 gaaaacttat taaaagaacg tataaaagaa cgaaaagaag aattttaccg agggttgcgt
      301 actgataagc taaggattag caaagaatta aatgaacaac tgaataaact ctatcaaact
      361 acgactgaag ttctgatgga aaaacacgag cagatccaac aagaattaca agcacaaatt
      421 ccaaaatgga aaaaagagta tgaaattcac caacaagaat tgaaagaaca agaagaaaaa
      481 caacggaaaa aagaaaaaga gcgcgaacaa ttagaactaa aaaaacgtga acaggatctt
      541 gcggaacaaa aaatggcgat agaaaaacaa cggctagaac aagaaaagga aaaagaagca
      601 gctgctcgta aggaacgtca agaacaaatg gatacattgc gacaagcaca gttaaattat
      661 ctgacaca
//
LOCUS       DACDYM010000232          607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDYM010000232 DACDYM010000000
VERSION     DACDYM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.249"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>607
                     /locus_tag="IWM59_002849"
     CDS             <1..>607
                     /locus_tag="IWM59_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980804.1"
                     /translation="LDYQISEGFYPTLIDLIRLDVFKDTDETMSVEESYRNEKLLQVL
                     EEELESDQRKVNQVSEVEVNEEVPWERPIFPKKESKEKETVYVDEQADIPTQKSIFEE
                     ENDVMGQLEVDPFEETMEDEETDEVEVDPVEANEESTWTEGDFQEYIEPLISEEVEKN
                     ETEIQKATVFDYILDQYPPEHEWMKEKNKLFLNELYSKYQLP"
BASE COUNT          240 a           78 c          139 g          150 t
ORIGIN      
        1 cactggatta tcaaatttca gaaggttttt atcctacatt gatagattta atccgccttg
       61 atgtgtttaa agatacggat gaaactatga gtgtggaaga gtcttatcga aacgaaaaac
      121 ttttacaagt tttagaagaa gagttagaga gcgaccaaag aaaagtgaat caagtgagtg
      181 aagtcgaagt aaatgaagaa gtaccatggg aacgaccaat ctttccaaag aaggagtcaa
      241 aagaaaaaga gacagtctat gttgacgaac aagctgatat tccgactcag aaatcgatat
      301 ttgaggaaga gaacgatgtg atgggtcaat tggaggtaga tccctttgaa gaaacaatgg
      361 aggatgaaga gacggacgaa gtagaagttg atccagttga agctaatgaa gagtcaacat
      421 ggactgaagg ggattttcag gaatatattg aaccacttat cagtgaagaa gtagaaaaaa
      481 acgaaacaga gatacaaaag gcaactgtat ttgattatat tttggatcaa tatccgccag
      541 agcatgaatg gatgaaagaa aaaaacaaat tatttttaaa tgagctttat tcgaaatatc
      601 aattacc
//
LOCUS       DACDYM010000233          607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDYM010000233 DACDYM010000000
VERSION     DACDYM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.250"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>607
                     /locus_tag="IWM59_002850"
     CDS             <1..>607
                     /locus_tag="IWM59_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980805.1"
                     /translation="LDYQISEGFYPTLIDLIRLDIFKDTDETMSVEESYRNEKILQVL
                     EEELESDQRKVNQVSEVEANEEVPWERPIFPKDESKEKEKGIIDEQADILTHKSIFEE
                     ENEVMGQLEVDPFEETMEDEETAEVEVDPVEANEDPTWTEGDSQEYIEPLISEEVEKN
                     ETEIQKATVFDYILDQYPPEHEWMKEKNKLFLNELYSKYQLP"
BASE COUNT          247 a           80 c          133 g          147 t
ORIGIN      
        1 cactggatta tcaaatttca gaaggttttt atcctacatt gattgattta atccgcctag
       61 atatatttaa agatacggat gaaaccatga gtgtggaaga gtcttatcga aacgaaaaaa
      121 ttttacaagt tttagaagaa gagttagaaa gcgaccaaag aaaagtgaat caagtgagtg
      181 aagtcgaagc aaacgaagaa gtaccatggg aacgcccaat ctttccaaag gatgaatcaa
      241 aagaaaaaga gaaaggaatt attgacgaac aagctgacat tttgactcat aaatcaatat
      301 ttgaggaaga gaacgaggta atgggtcaat tggaggtaga tccctttgaa gaaacaatgg
      361 aggatgaaga gacggccgaa gtagaagttg atccagttga agctaatgaa gacccaacat
      421 ggactgaagg ggattctcag gaatatattg aaccacttat cagtgaagaa gtagagaaaa
      481 atgaaacaga gatacaaaag gcaacggtat ttgattatat tttggatcaa tatccgccag
      541 agcatgaatg gatgaaagaa aaaaataaat tatttttaaa tgaactttat tcgaaatatc
      601 aattacc
//
LOCUS       DACDYM010000234          602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDYM010000234 DACDYM010000000
VERSION     DACDYM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.251"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..322
                     /locus_tag="IWM59_002851"
     CDS             <1..322
                     /locus_tag="IWM59_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9980806.1"
                     /translation="EALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIY
                     STNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTL
                     ESMI"
BASE COUNT          214 a          106 c           75 g          207 t
ORIGIN      
        1 agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt atccaagagt
       61 cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc acgccatcag
      121 acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa aacgatatac
      181 tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca tttctcaatt
      241 taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag aaattcagga
      301 tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt tacatataat
      361 tcttgacgct accataatct aaataaccat catattcatc tactacttat ataattgagt
      421 tttattaaac tttttaagtt tctattctat caaatattta aacaatctct gcatccgaaa
      481 agactacctc gtatagtttt actaacaata tttttattag cgtgttcaaa tcctgatttc
      541 ttgtatcaat tattttctgt tcactttcaa taagaaaaaa gtctttactt ccatggataa
      601 ta
//
LOCUS       DACDYM010000235          587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDYM010000235 DACDYM010000000
VERSION     DACDYM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.252"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..293)
                     /locus_tag="IWM59_002852"
     CDS             complement(<1..293)
                     /locus_tag="IWM59_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980807.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTP"
     gene            complement(311..553)
                     /locus_tag="IWM59_002853"
     CDS             complement(311..553)
                     /locus_tag="IWM59_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980808.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
BASE COUNT          171 a          127 c           96 g          193 t
ORIGIN      
        1 tgtggggttt gtccaaacat tgagaacatt gaaactacaa tagaagcggc agctaaaata
       61 actcctaccc ccaacaatac ataacctacg tgtgtcttcc cgttttcagc tcctcttcgc
      121 ccaaagaaga agaaaatacc tccaagagca atggaagcca ctgccgcaac agcagcaaaa
      181 atcatcagat tatttttcaa actagttgcg gcagtttgta atgctctaaa aatatcaacc
      241 gcagctacta ctttcggttg tgttaaaaaa ttcattgtaa ttgattcaaa cataaaattg
      301 ttcgctcctt ttagtgttta ataatttcat cgaaaacaag cttttcacgt tcacgttgat
      361 agatatatcc ttctaagtcc ttgtcttttc cccgtaatag gtcaaattgt gctttttcaa
      421 ggacttcttg tttccactgt gtttcagata caccgcggcg ttctaagatt ttagacgcgg
      481 ctttttcaaa tggcgtttta gcttttgcca ctgtgattcc tccttcagga caaataaaaa
      541 gactagcaat cactcgctag tcttttgtaa tttgtattta tttgatt
//
LOCUS       DACDYM010000236          585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDYM010000236 DACDYM010000000
VERSION     DACDYM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.253"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          179 a           92 c           63 g          251 t
ORIGIN      
        1 catttggttt gtcttacttt gttcagtttt caaaggtcta atctttttgt tttaacaact
       61 ctttaatctt atcagattca gaagagaatg tcaacaactt ctttgtttat tttttctaaa
      121 cgtctttgcc actcattagc agcgacttga ttatcatacc atctttattg ttatttgtca
      181 ataacttttt cgaagttttt ttcgataaaa tcaagcttta ttagctatct catccaaacg
      241 acttcgttat tataacatga catcaaaagt gcgtcaacac tattttttat aatttttagt
      301 gacaccttaa aggaatcatt tagaacaaat aataatatag caatctcttt ataaaattgc
      361 aatagaaaat tccctatttt tatttattat ttttaaacac gaatattaca gcattttatt
      421 gttttttcgt tcatctaaaa gatacttaca tttaattaac ggatgttatt ctatttaatt
      481 gttcgctagt ttttttataa tgacatccct gaatttctca cttgcttgta ttttgtacct
      541 tattctttgt tggatatata aaaaaacaag taaagcacat gcttt
//
LOCUS       DACDYM010000237          581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDYM010000237 DACDYM010000000
VERSION     DACDYM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.254"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            108..>581
                     /locus_tag="IWM59_002854"
     CDS             108..>581
                     /locus_tag="IWM59_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9980809.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSK"
BASE COUNT          202 a          112 c          108 g          159 t
ORIGIN      
        1 caccgtttta tttgtctcac acccttttcc ttcctacttg taaccttgct cccgtgttag
       61 aaacatgtta cactaaatat gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa
      121 cagatacact gccaaaagca actgcgaaaa gattaccttt atatttacgc tacttgaaga
      181 tgctggatga ctcaggtata agtagaatca aatcaaatga gttcagcgaa atcacccaaa
      241 ttccttctgc aacgatccgc agagacttct cgcaattagg cgagctaggc agaagcggat
      301 atggctatga tgtccctttt cttatcgatg tattcaataa tatccttaat actaaagaag
      361 aaaaacgaat cgcattagta ggatatggaa atcttggcaa agcattgaaa cacaacaatt
      421 ttagaagaaa tgaaaatcta aatatcgtgt gcgtctttga taatgatcca gcactgatca
      481 atcgagtaat tgatggagaa atgatctatc cgattgatcg tttcgcggag atagccaaag
      541 caaaaaatgt gacagtagca atctctacgg taccaagtaa a
//
LOCUS       DACDYM010000238          569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDYM010000238 DACDYM010000000
VERSION     DACDYM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.255"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            73..>569
                     /locus_tag="IWM59_002855"
     CDS             73..>569
                     /locus_tag="IWM59_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP9980810.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          212 a          105 c           93 g          159 t
ORIGIN      
        1 catacaaaaa aggaagtccc ttctgtagaa taaagttaac gacaaccaat tcacagaaaa
       61 gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt cactaaaggc
      121 gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact cctacggact
      181 gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat
      241 tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt aacattggaa
      301 atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta caaaagaaca
      361 aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac gatgtctgaa
      421 attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac catgtccaac
      481 atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt aaatgataag
      541 tatgtcgcta tttttatgga cgctactta
//
LOCUS       DACDYM010000239          568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDYM010000239 DACDYM010000000
VERSION     DACDYM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.256"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>568
                     /locus_tag="IWM59_002856"
     CDS             <1..>568
                     /locus_tag="IWM59_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9980811.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKH"
BASE COUNT          212 a          109 c          103 g          144 t
ORIGIN      
        1 aaaggaaaag gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt
       61 accatgagtt accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt
      121 ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa
      181 caagtaattg ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa
      241 gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca
      301 aaattggcaa aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg
      361 caaaatacac ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct
      421 gccaactctg taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag
      481 gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt
      541 ctggtggata cgctactacg taaacacc
//
LOCUS       DACDYM010000240          556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDYM010000240 DACDYM010000000
VERSION     DACDYM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.257"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(152..346)
                     /locus_tag="IWM59_002857"
     CDS             complement(152..346)
                     /locus_tag="IWM59_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980812.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT          171 a          113 c           88 g          184 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat
      181 cgacgaacgt cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg
      241 ccgctttgtc tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat
      301 tgtctctgct tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc
      361 ttctctttct tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa
      421 caattcattg aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      481 tcacttaaca actaacactc tgtgagtgaa atggactctt caatgtgatg ttaggatgat
      541 ttcatggaca cgattt
//
LOCUS       DACDYM010000241          548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDYM010000241 DACDYM010000000
VERSION     DACDYM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.258"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>548)
                     /locus_tag="IWM59_002858"
     CDS             complement(<1..>548)
                     /locus_tag="IWM59_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9980813.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DACDYM010000242          541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDYM010000242 DACDYM010000000
VERSION     DACDYM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.259"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>541
                     /locus_tag="IWM59_002859"
     CDS             <1..>541
                     /locus_tag="IWM59_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9980814.1"
                     /translation="TPPEKVNITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTK
                     YNKADYGDVEFTLGNITDQVLPREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKIT
                     SAVDENGEITFTDQPAYVNSKGNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGF
                     LKNIHLYPKNIVSKLSFELT"
BASE COUNT          201 a           85 c          114 g          141 t
ORIGIN      
        1 acaccaccag agaaggtcaa tatcacggtt cataaattaa tgtacgatca aggaacgcaa
       61 ttgaatgttg atattgacgg gatcaaaaac gatggttata cgcacgatca atatcctaca
      121 ggtgtaacga aatacaacaa ggcagattat ggtgatgttg agtttacgtt aggaaatatt
      181 acggatcaag tattaccgag agaagattct gatctaacca acgcgaaagt ggacgagatt
      241 gtacaagatg tagaagacaa gggatctaac agtgagtacg tcaagaatgc aaccaacaaa
      301 attacttcag ctgtagatga aaatggtgag atcactttta ctgatcaacc agcctatgtc
      361 aattccaaag ggaatgtata tgtggtttat gaaagtaaat ccgctgctgg attagtcaca
      421 caaaaagcaa aaccaatggt tgtgattgcg ccaatgacag ataaaacagg atcaggattt
      481 ttaaaaaata ttcatttgta tcctaagaac attgtttcta agttgagttt tgaattaacg
      541 a
//
LOCUS       DACDYM010000243          531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDYM010000243 DACDYM010000000
VERSION     DACDYM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.260"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>531)
                     /locus_tag="IWM59_002860"
     CDS             complement(<1..>531)
                     /locus_tag="IWM59_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP9980815.1"
                     /translation="YQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNK
                     TVEQENSTINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIR
                     PYPIFNHGETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYA
                     RNLYHGLHDQLEEKNEE"
BASE COUNT          158 a           92 c           85 g          196 t
ORIGIN      
        1 ttcttcgttt ttttcttcta attgatcatg cagtccatga tagagatttc tagcatattc
       61 atctggtagg aaataatcat aatcaatttg taaagcctta agatccaagt ccgcatgtgt
      121 acaatgtatg tctatatcat tgatatccca cgatggattg aaatcttcaa tatattgata
      181 agcataagga agattcgttt ccccatgatt aaaaatagga tacggccgta ttttatgacc
      241 tttcaaatct tgtcgtttta aaggagcgag tacaatcgtt tcaccttcta aaaaagtaga
      301 aattctttca ggagttaaga tacgatctga atcaacattg cgattgatat gagtattcat
      361 acctaatgaa ttgatggtac tgttctcttg ttcaaccgtc ttattcccca cttttttaga
      421 aaattcttcg atggtattat catctgtcga cataatataa atatgtgtct gacaattttc
      481 tttgataatc gctgcgtttt gttctccata cttcgcataa agttgctgat a
//
LOCUS       DACDYM010000244          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDYM010000244 DACDYM010000000
VERSION     DACDYM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.261"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IWM59_002861"
     tRNA            complement(5..90)
                     /locus_tag="IWM59_002861"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IWM59_002862"
     tRNA            complement(107..178)
                     /locus_tag="IWM59_002862"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IWM59_002863"
     tRNA            complement(190..262)
                     /locus_tag="IWM59_002863"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IWM59_002864"
     tRNA            complement(280..361)
                     /locus_tag="IWM59_002864"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IWM59_002865"
     tRNA            complement(369..441)
                     /locus_tag="IWM59_002865"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IWM59_002866"
     tRNA            complement(445..517)
                     /locus_tag="IWM59_002866"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDYM010000245          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDYM010000245 DACDYM010000000
VERSION     DACDYM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.262"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..354
                     /locus_tag="IWM59_002867"
     CDS             <1..354
                     /locus_tag="IWM59_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9980816.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DACDYM010000246          523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDYM010000246 DACDYM010000000
VERSION     DACDYM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.263"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..523
                     /locus_tag="IWM59_002868"
                     /pseudo
     CDS             1..523
                     /locus_tag="IWM59_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           89 c           99 g          123 t
ORIGIN      
        1 aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa atcattcgag aatatgaact
       61 aacggcttca gcatttgaca aatgggtaaa gcaatctaaa acgagtggtt cattcaaaga
      121 aaaagataat cttacgcctg aacaaaaaga attgttagaa ctacgtaaaa gaaaccagca
      181 attagaaatg gaaaatgata ttttaaagca agcagcgctg atattcggac gaagagacaa
      241 gtaatcgatg cgaataagca tctttaccct atatcagcga tgtgcagaat attaggtcta
      301 tcacgtcagt cctattatta tcaatcaaaa ccaaagaaag acgaatcaga acttgaagaa
      361 gtagtcgctg aagaatttat ccgcagccga aaggcctacg gctcaagaaa aataaaaaaa
      421 gccttatcaa aacgaggcat tcagatcagc cgacgaaaaa ttagtagaat catgaaaaat
      481 agaggattaa aatcgagcta tactgttgct tattttaaag tac
//
LOCUS       DACDYM010000247          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDYM010000247 DACDYM010000000
VERSION     DACDYM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.264"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            57..>517
                     /locus_tag="IWM59_002869"
     CDS             57..>517
                     /locus_tag="IWM59_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9980817.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DACDYM010000248          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDYM010000248 DACDYM010000000
VERSION     DACDYM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.265"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          130 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactct
//
LOCUS       DACDYM010000249          500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDYM010000249 DACDYM010000000
VERSION     DACDYM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.266"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>500
                     /locus_tag="IWM59_002870"
     CDS             <1..>500
                     /locus_tag="IWM59_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980818.1"
                     /translation="SLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFK
                     IEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEI
                     VILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDS
                     DPLLQY"
BASE COUNT          179 a           82 c          100 g          139 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta
//
LOCUS       DACDYM010000250          479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDYM010000250 DACDYM010000000
VERSION     DACDYM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.267"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>479)
                     /locus_tag="IWM59_002871"
     CDS             complement(<1..>479)
                     /locus_tag="IWM59_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP9980819.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     "
BASE COUNT          141 a           91 c           85 g          162 t
ORIGIN      
        1 cgttcattat tgtagtaata gatatagcta tcgatggctg attttaattc ctcgaaacag
       61 ttataaactt tcccgtgaaa aatctcttgt tttaataagc caaaaaaatt ctccataagt
      121 gaattatcta agcagttccc tttccgagac atgctttgaa agattttgta ctccttcaat
      181 ttgtttctat aagctttcat ttgatacgcc caaccttgat ctgagtgaaa cgtacgacga
      241 tagggacaat cgttagtggt ctggatagct tcctccaaac cttccattac cgctaatgca
      301 ttgggctttt cagagatacg ataggataaa atctcactat tgaacatatc cataaaagga
      361 tctagataca atttcttttg acggataatg cctttggcat ccacttcatg gtatttaaat
      421 tcagttgtat ccgtcgtgat tttctgatgg cagatactgg tgtagaaacg acgatggat
//
LOCUS       DACDYM010000251          473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDYM010000251 DACDYM010000000
VERSION     DACDYM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.268"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IWM59_002872"
     CDS             complement(<1..339)
                     /locus_tag="IWM59_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9980820.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
     gene            complement(356..>473)
                     /locus_tag="IWM59_002873"
     CDS             complement(356..>473)
                     /locus_tag="IWM59_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9980821.1"
                     /translation="QFLWKEMFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          139 a           81 c           76 g          177 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
      421 ttgtcagaag acaaaaactt ttagaccaga acatttcttt ccaaagaaat tgt
//
LOCUS       DACDYM010000252          468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDYM010000252 DACDYM010000000
VERSION     DACDYM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.269"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>468
                     /locus_tag="IWM59_002874"
     CDS             <1..>468
                     /locus_tag="IWM59_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9980822.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DACDYM010000253          467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDYM010000253 DACDYM010000000
VERSION     DACDYM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.270"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>467
                     /locus_tag="IWM59_002875"
     CDS             <1..>467
                     /locus_tag="IWM59_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9980823.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSV"
BASE COUNT          160 a           90 c           95 g          122 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtg
//
LOCUS       DACDYM010000254          463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDYM010000254 DACDYM010000000
VERSION     DACDYM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.271"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(204..>463)
                     /locus_tag="IWM59_002876"
     CDS             complement(204..>463)
                     /locus_tag="IWM59_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9980824.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          165 a           59 c           80 g          159 t
ORIGIN      
        1 gaattaaaaa tattaaacag gaatttatcg aaaatggtag aaaagaaatt tataaaagga
       61 cttataaaga ttgattatat aatatatgaa aagctattat aaaagacatt agtattaaat
      121 agtttaaaaa aatgaaaaat aataaaggaa gtgagtcaat tccagactcc tgtgtaaaat
      181 gctatacaat gtttttacca tttctactta tcaaaattga tgtattttct tgaagaataa
      241 atccattcat catgtaggtc cataagaacg gctccaatta agcgattggc tgatgtttga
      301 ttggggaaga tgcgaataat cttttctctt ctgcgtactt cttgattcag tcgttcaatt
      361 agattggtac tctttagtcg attgtgggaa tttccttgta cggtatattg aaaggcgtct
      421 tcgaatccat catccaatga tgcgcaagct tttgaatatt ttg
//
LOCUS       DACDYM010000255          435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDYM010000255 DACDYM010000000
VERSION     DACDYM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.272"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>435)
                     /locus_tag="IWM59_002877"
     CDS             complement(<1..>435)
                     /locus_tag="IWM59_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9980825.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLS
                     YNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFK"
BASE COUNT          122 a           77 c           97 g          139 t
ORIGIN      
        1 tttaaacgac tgaggtaacg tagatgaaat tgttttaagg gtttcttgaa agccttccgt
       61 acctcgttta acgaaataat agcgtaataa ttgacaaaaa tgatacgcag ttcgcaattc
      121 ttcgttgtag cttaacagct catcaacgat ttctgtttga ctaattggtc gtttaaacag
      181 tcgatgataa tgccgtttga gtggctccca tgtcgtggaa tctttgagta agagtttcca
      241 atatcgcttg agccgacggt acttcttctg attttcagag tgtgaggttc gaaaacgatt
      301 catgatctgg acacgtaatt gattgaagct gcgattgatg tgttggacaa tatgaaagcg
      361 gtcagttacg atctccgcat ttggaaagac tgttttaagc aactgacaat agctggcatt
      421 catgtccatc actag
//
LOCUS       DACDYM010000256          420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDYM010000256 DACDYM010000000
VERSION     DACDYM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.273"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            74..403
                     /locus_tag="IWM59_002878"
     CDS             74..403
                     /locus_tag="IWM59_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9980826.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DACDYM010000257          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDYM010000257 DACDYM010000000
VERSION     DACDYM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.274"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..379
                     /locus_tag="IWM59_002879"
     CDS             <1..379
                     /locus_tag="IWM59_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP9980827.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DACDYM010000258          406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDYM010000258 DACDYM010000000
VERSION     DACDYM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.275"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>406)
                     /locus_tag="IWM59_002880"
     CDS             complement(<1..>406)
                     /locus_tag="IWM59_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9980828.1"
                     /translation="YFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFGDVDSPVRRA
                     FLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCIFTGLFSINTNE
                     SSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYR"
BASE COUNT          163 a           83 c           71 g           89 t
ORIGIN      
        1 gacgataaag cagaacaagt aacaatggga aaagtagaaa gcctgcatag cctagcccag
       61 aacctgtatt atggatccaa gtcgatagat tccaagagct ttcattcgta ttgatactga
      121 ataacccagt aaaaatacaa tcacctatcc catataaacc aactgttgca acagcaagta
      181 ttgcaagtgt ttttgatgtt ccaacaaata ggtgatacaa cgcaggcaac gaaagaacaa
      241 aaaaaagtcc agatacaact gaccagacca agaaagcacg tctgactgga ctatctacgt
      301 caccaaacac actaattacg gtagtcattt gattcagttt gggataaaaa aggccaagga
      361 tatacggtgt cgataaatca ctaatcacac ctaataataa aaaata
//
LOCUS       DACDYM010000259          389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDYM010000259 DACDYM010000000
VERSION     DACDYM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.276"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>389
                     /locus_tag="IWM59_002881"
     CDS             <1..>389
                     /locus_tag="IWM59_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP9980829.1"
                     /translation="NVAKFQQQQRILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIS
                     LKLPIYDGTSDKILENGVGITEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKG
                     QKFFIKVNEEQHAYQIYRIEEVKAEEL"
BASE COUNT          146 a           55 c           77 g          111 t
ORIGIN      
        1 aatgtagcga aatttcaaca acagcaaaga attttagcca ttgaaaatct tgaaaaaaca
       61 gattcttctt tggatgttgc gaaaatagag ttaggcgatc ctgttggtgt tcttacaatt
      121 ccaagtattt ctttaaaact accaatttat gacggaactt ccgacaaaat tttagagaat
      181 ggtgtaggga ttacggaagg aactggcgat attacaggag gaaacggtaa aaatccttta
      241 atcgcggggc acagtggact ttataaagat aatcttttcg atgatttgcc ttcagtaaaa
      301 aaagggcaaa aattctttat taaggtaaat gaagaacaac atgcctatca aatttatcga
      361 attgaagaag ttaaagcaga agaattaca
//
LOCUS       DACDYM010000260          369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDYM010000260 DACDYM010000000
VERSION     DACDYM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.277"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>369
                     /locus_tag="IWM59_002882"
     CDS             <1..>369
                     /locus_tag="IWM59_002882"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP9980830.1"
                     /translation="KRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWL
                     KTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNR
                     NRIHSSIHYLTPQEFEELAK"
BASE COUNT          147 a           69 c           65 g           88 t
ORIGIN      
        1 aaaacgaatg actgtggatt gtgtcattca aacattgaat aaagcaaaaa tacattatca
       61 cattccagaa ggaatgattc tacacactga cttgggcagc caatacacag caagagaagt
      121 ggaacaatgg cttaaaacca acaaaataag gcattcttat agtagaaaag gaacacctta
      181 tgataatgct ggaatcgaat ctttccatgc ctcattgaaa aaagaagaag tctacacgac
      241 tagctactca gattttgaag aagcaaatcg agcgctattt agctacattg aaggatttta
      301 taaccgaaat cgaatccata gctcgattca ctatctaacc ccacaggaat ttgaagaatt
      361 agcaaaaga
//
LOCUS       DACDYM010000261          368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDYM010000261 DACDYM010000000
VERSION     DACDYM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.278"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..242
                     /locus_tag="IWM59_002883"
     CDS             <1..242
                     /locus_tag="IWM59_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980831.1"
                     /translation="KSASKKLTINIDELADQVLSQLNETQEEYYTMPANDAKDNVSHR
                     FPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          128 a           69 c           68 g          103 t
ORIGIN      
        1 ataaaagtgc ttcgaagaaa ttaacgatca atattgatga gttggcagat caagtactct
       61 ctcagttgaa tgaaacgcaa gaggaatatt acacgatgcc agcaaatgac gccaaagata
      121 atgtttctca tcgttttcct ttcagtagac gcatctactc agatgaaaat gacggcacat
      181 tgaatacaga gtacacatat gaaggtcagc catatgtgct agaaactgat gatgatcttt
      241 aaagacagtt tttgaaaagg ccgctcatcc ttaagtgatg acaaaaaatc atcacaatta
      301 ccgatgactg tctctttact atcttttaat cagaaaaagg ggttatgaca agctttgtca
      361 accccttt
//
LOCUS       DACDYM010000262          368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDYM010000262 DACDYM010000000
VERSION     DACDYM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.279"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>368
                     /locus_tag="IWM59_002884"
     CDS             82..>368
                     /locus_tag="IWM59_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9980832.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          130 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcg
//
LOCUS       DACDYM010000263          342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDYM010000263 DACDYM010000000
VERSION     DACDYM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.280"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..270
                     /locus_tag="IWM59_002885"
                     /pseudo
     CDS             1..270
                     /locus_tag="IWM59_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876065.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          124 a           46 c           64 g          108 t
ORIGIN      
        1 aaatctatat tttgatatac aaattacagc ttcagatact aagttgcata aaccttttcc
       61 ggaccccata caaaaggcaa ttgatgaatt gggcataaaa aataagaaac gatttatatt
      121 ggtgattcat tatatgacat gcaaagtgcg aaagcttgtg ggatagcatt tggtttagct
      181 aaatggggag ccaaaaatat agaaaatttt caaagtgtag atataattat tgattctcct
      241 caagaaatac ttgatttgtt agaaaaatag acaaagagcg tgagataagg atagggtctc
      301 acgctctttg tttttcgcca tttaaagatt gaaatttttg cc
//
LOCUS       DACDYM010000264          340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDYM010000264 DACDYM010000000
VERSION     DACDYM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.281"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>340
                     /locus_tag="IWM59_002886"
     CDS             <1..>340
                     /locus_tag="IWM59_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980833.1"
                     /translation="AYIRLYSGVLHLRDSVRVSEKEKIKVTEMYTSINGELCKIDRAY
                     SGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALLE
                     ISDSDPLLRY"
BASE COUNT          126 a           48 c           64 g          102 t
ORIGIN      
        1 ttgcatatat acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag
       61 aaaaagaaaa aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaga
      121 ttgatagagc ttattctgga gaaattgtta ttttgcaaaa tgagtttttg aagttaaata
      181 gtgttcttgg agatacaaaa ctattgccac agagaaaaaa gattgaaaat ccgcaccctc
      241 tactacaaac aactgttgaa ccgagtaaac ctgaacagag agaaatgttg cttgatgccc
      301 ttttggaaat ctcagatagt gatccgcttc tacgatatta
//
LOCUS       DACDYM010000265          332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDYM010000265 DACDYM010000000
VERSION     DACDYM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.denovo.282"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>332
                     /locus_tag="IWM59_002887"
     CDS             <1..>332
                     /locus_tag="IWM59_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980834.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DACDYM010000266        12268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.076, whole genome shotgun sequence.
ACCESSION   DACDYM010000266 DACDYM010000000
VERSION     DACDYM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.076"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..816
                     /locus_tag="IWM59_002888"
     CDS             <1..816
                     /locus_tag="IWM59_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9980835.1"
                     /translation="TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGL
                     LQSLGASLEGYTEGNPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFG
                     RQKGGIDTTLAFLDKQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSG
                     FDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSR
                     DQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1074..2576
                     /locus_tag="IWM59_002889"
     CDS             1074..2576
                     /locus_tag="IWM59_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9980836.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2643..3170
                     /locus_tag="IWM59_002890"
     CDS             2643..3170
                     /locus_tag="IWM59_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9980837.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3381..4442
                     /locus_tag="IWM59_002891"
     CDS             3381..4442
                     /locus_tag="IWM59_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9980838.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4445..5341
                     /gene="murQ"
                     /locus_tag="IWM59_002892"
     CDS             4445..5341
                     /gene="murQ"
                     /locus_tag="IWM59_002892"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9980839.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5355..7337
                     /locus_tag="IWM59_002893"
     CDS             5355..7337
                     /locus_tag="IWM59_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9980840.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7402..7848)
                     /gene="nrdI"
                     /locus_tag="IWM59_002894"
     CDS             complement(7402..7848)
                     /gene="nrdI"
                     /locus_tag="IWM59_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9980841.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            8059..9483
                     /locus_tag="IWM59_002895"
     CDS             8059..9483
                     /locus_tag="IWM59_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9980842.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(9664..10437)
                     /locus_tag="IWM59_002896"
     CDS             complement(9664..10437)
                     /locus_tag="IWM59_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9980843.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(10434..11339)
                     /locus_tag="IWM59_002897"
     CDS             complement(10434..11339)
                     /locus_tag="IWM59_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9980844.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(11493..11813)
                     /locus_tag="IWM59_002898"
     CDS             complement(11493..11813)
                     /locus_tag="IWM59_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9980845.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         3958 a         2163 c         2620 g         3527 t
ORIGIN      
        1 acggatcaaa ctatccgact tgcttccagt tatgccttag gagaacttat taaagatgca
       61 ctgcttcaag aagtagaaca aattattgtc actttaggag gaagcgggtg tagcgatggt
      121 ggtctaggat tactccaatc cttgggtgct tctttagaag ggtacacaga aggaaatcct
      181 ttattatcta ctaaaaaact tcattttgat cgaagaaacg ttcttgtttc taaagtaaaa
      241 ttgattatcg ctgcagatgt taccaatcca tatacaggtc aacaaggagc tgcttggcaa
      301 tttggaagac aaaaaggagg aatagatacg acgctagctt tcttagataa acaagcagga
      361 aagattgctc agcaaataaa agatttctgg gaggtggatt tagatgatat tccaggaagt
      421 ggagcagcag gcggtattgg tggagccctt tttttgttgg gagcgaagat ggtttctggt
      481 ttcgatttag tcagccaaat cactggattg gaaaaaaaga tcgaacaagc agatgtcatc
      541 attacaggag aagggcggat cgatcagcaa acgatccacg gaaaagttcc atacggtgta
      601 gcagtgcttg caaaaaaata tcgaaaacca ataatcgcta tttgcggaag tagagaccaa
      661 gagctagaag gactttctga ttttctccca attgtcttaa gtattcattt gggtccaata
      721 ggtttagaag aagcaatgga acacaggcaa acattagaaa aaacaaaaat cttaggacag
      781 acacttagca gatttttcac catcctcccg aagtagttga caaaatcatt ttctttatgc
      841 tatcgtaatt tcaaatatag gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat
      901 tgatgaaagc tgcattcaga ctgtaccttg tggagacaag gaagcagtgt gcagattttc
      961 gggattggct tggttttttc gatgtatggg tctttttgtg tgtatccaac ccttgcatcc
     1021 tctttagcca gttctgaaaa taggactggt ctttttttac gaggtgatgg aaaatgtcta
     1081 aaatcgaaat aaaaaatctg acattcggct acgacagcca aggcacatta ttatttgaac
     1141 aagcaaatct aaattttgac acacaatgga aactaggact tatcggacga aacggtcgag
     1201 gaaagacaac tttactgaat attctacaaa acaaactacc ttatcaaggg caagtaatcc
     1261 atcagcaaga atttgcctat ttcccgcaac agacaaaaga taaagaacgt ttaacctatt
     1321 acgtgttaaa tgatattacg gattttgaga tatgggaaat cgaaagagag ctccaattga
     1381 tgcaaacaga tcctgaaatc ttatggagag aattcagcac actatcgggg ggagagaaga
     1441 caaaagtcct actggcactt ttatttgtgg atgacactca tttcccgtta atcgatgaac
     1501 caacgaatca tttggatatc tctggtagaa aacaagtagc ggcttatttg aaaaagaaaa
     1561 aacaaggctt catcgtggtc agccatgacc ggggatttat cgatgaagta gtggaccatg
     1621 ttttagcaat cgaaaaaagt caactggaac tttatcaagg gaatttctct atctatgaag
     1681 aacagaaaaa acttcgtgat gaatttgaaa tggctcaaaa tgaaaaattg aaaaaagaag
     1741 tcagtaggct aaagaaaaca gcagctgaaa aagccgaatg gtctcgttcc cgagaaggag
     1801 ataaaacaaa gaaacaagtc ggattcatcg atactgaatc tagacgagtg aataaaggag
     1861 cagtgggtgc tgatgctgca cggacgatga aacgatccaa agcaatcgtg aatcggatgg
     1921 agacccagat cagcgagaaa gaaaaactat taaaagatat cgaatatatc gattctttga
     1981 cgatgaatag ccaagcgtct caccataagc gacttttaag cgtagaagat cttcaattag
     2041 ggtatgaaaa tctgttattc gagccaattc attttacaat cgagcctcat cagcgggtgg
     2101 cgatttcagg tcctaacggt gcaggaaagt catccattat ccattatctt ctgggggcat
     2161 tcaacggcaa ggttatagga gaaaaatacc agccaaaaca tctgagcatt agttatgcaa
     2221 gccaaaatta tgaagacaat cgaggaacgt tggcggaatt tgcagagaaa aaccaagtag
     2281 actaccaagc atttttgaac aacctccgaa agcttgggat ggaaagagat gtttttcata
     2341 acaagatcga gcagatgagt atgggccaac ggaaaaaagt ggaattggct aaatctttat
     2401 cacagccagc tgaactatat acatgggatg aaccattgaa ttatttggat gtcttcaatc
     2461 aagaacaatt agaacaactg atcttgaacg tgaaacctgc catgttacta gtggaacatg
     2521 atcaaacctt cctggataaa gtatctactg agattatttc tcttgagaga atctaaccct
     2581 tgtaatctca agaacaacaa ggtatggtta aggggaactt tgccaacgaa ggagagaatc
     2641 acatgcttta tgtaacgaga cacggagaaa caacttggaa cgcacaagga ttagtttgcg
     2701 gacgggcaga tgtgccgctg actgaaaaag gacagatgca ggcacagaaa ctagcagaaa
     2761 aggtagtaga tttgcctgtt ccaatcacga aaatcatcca ttcccctctt caacgagcaa
     2821 gagatacagc acaagcagtt gctgatcgtc tcagcttgcc gctgaccgtt gatgaacgat
     2881 tggtagaaat ggattttggg gattatgatg gactgccaag caaagatgag aactttcaaa
     2941 aagcacgttt agcgttcgct gtacgattcc caaatgggga gtcagtctta gatgtgtatg
     3001 cacggatcgt tcctttattg aaagaatgca tcgaagacga agaaaatgtc tatctgttag
     3061 tttgccataa tgccttgata agagtgatca atgcttattt tcatccgatg cctaatgagg
     3121 ggttctttac attcatggta gataatacag aattggtttc ttatgagtaa ttatgttttt
     3181 gtgttattta aataaaatat ttcaaggcac tgcttttgct tagcagtgcc ttttttaggt
     3241 tatgacaatt cttttgataa tataaactca acctttagaa atgaaattta attattttaa
     3301 taaaagtatt gaaattttgt ttctttagat atatactcta gtcatagaaa tgaagacgga
     3361 tacagaaaaa gagggataat atgtttggct tttcaatatt tatgaacgat tctttgtcag
     3421 aagagaagaa acagtatatc cgccaaatgg catccaatgg ctttgtcggt attttcactt
     3481 ccatgcatat accagaagat gatgtttcgg catatcaaaa gcgattgatc gaactaggaa
     3541 gcgaagcgaa aaagaataaa ctagaattga tggtcgatat ttcaggagag gcattggaac
     3601 gagcgggttt ttcattagaa gatctaacgc ctttgaaagc aattggtgta acaggtttaa
     3661 gaatggatta tcatatttcc aatcagcaga ttgccgactg gtcacatcaa ttgaaaatca
     3721 gtttgaatgc tagcacgatc acgcctaaag atatcgatga attgaaagaa gcagaagctg
     3781 atttctcaca gattgaagct tggcataatt attatccacg tcccgaaaca gggctggaca
     3841 aagaatggta tcagaaaaaa aatcaatggc tcaaaattca aggtctcctt gtccaaggtt
     3901 ttgtaccagg agatacagag ttgagaggcc cactatatca aggattgcca actttggaag
     3961 aacatcgagg gatccatcct cttgcaggcg ctctagatct attagcaagt aacacggata
     4021 ttgtttacat cggagatgca ggtctgtcag aaaacgttca ggagcaattt gcttctttcc
     4081 aaaaagaaca gacagtcttg ctccatacgg aacctgtaga tgaagaattt tacgaatata
     4141 tattagggaa gcatacgaat cggcaagacg atgcaagaga tgtcatacga agcgtagatg
     4201 cacgttttcg agagattccg cctatcccag caagaaatac agctacgcgt atgaaagggt
     4261 ctattacttt agataacgaa aagtatttgc gctatatggg tgagatccaa ttaaccaaat
     4321 atgatttgcc agcggatgaa aaagtgaatg tagtagctaa agttatcaaa gaagagctac
     4381 cattgatcaa ccaaatcaaa gctggtatga actatcaatt tataagaaaa gaaggaagat
     4441 aaaaatgagc aaaatcaatt tagagaacct aacaacggaa cgcagaaacc aagaaacttt
     4501 tggattagat gagatgagta tttctcaagc cgttcaacta atgaacaaag aagatcagaa
     4561 agtggccgct gcggttgaaa aagaactgac aagtatcgaa ccagtgattg ctaacacgat
     4621 tgagtccttt aaaaaaggtg gacgtctgat ttatatggga gccggaacaa gtggaagact
     4681 aggtgtgtta gatgctgctg aatgcgtacc gacattcggt gtagaaccgg aaatggtcat
     4741 aggtctaatt gctggtggag aagaagcaat gactgtagct gtcgaagggg ccgaagattc
     4801 ttatgagcta gggcaagaag atctaatcaa gctgaatttg acaaaaaatg atatagttat
     4861 tggtatcgca gctagcggtc gtacaccata tgtgatcggt ggattagatt acgcaaaatc
     4921 gatcggtgct gtcacaggaa cgatttcttg caataaacaa gccgaaatca gtaaacacgc
     4981 ggatttccca atcgaagtcg atgcaggtcc agaatttttg acaggctcta ctcgattgaa
     5041 gtcaggaaca gcacaaaaac ttatcttgaa tatgatttct actatcagta tgattggtat
     5101 aggtaaagta ttcaataact tgatggtcga tgtcaaacca acaaatgaaa aactaatcga
     5161 acgctcaaaa cgaatcatca tgcaagcgac agaagctgat tacgaaacag ctgaaaagta
     5221 ctttgaagaa gcagatcaaa atgttaagct ggctatcgtc atgattttga caaatagtga
     5281 taaagaggaa gctcaagcaa aattagctga agcaaacgga tttgtaaaag ggaccttatg
     5341 agggggaaaa gaccatggca aaaaacgaac tgactttgga agaactggca aaacaaattt
     5401 ataaaggggc aggcggcatg ggcaatgtag aaagcattgt ccactgcatg actcgtgtgc
     5461 gtatgaagat catcaatgat gatcttgtaa atgtctctga attaaaagcg attccaggag
     5521 tgcttggtgt agtagaagat gaacagcttc aagtcatcat cggacctggg aaagtgaaca
     5581 aagtagcaca gcaaatggtt gatttggcag gcgtgaaatt aggagaaaaa ctttcttctg
     5641 taagtacagc tacagcaaat tcatcagcat ctggaaaaga ccgtgtaaac gaacgggcac
     5701 aagaaatgaa agcggcacaa aaatcgaaac aaaaaccatc taaaatcaaa tcggtattaa
     5761 aagacatttc taatatcttt gtgccaatga ttcctgcatt tgtcggtact gggatcgtgg
     5821 cagggattgc cgcagtttta gcaaacttag tcacagccgg aacacttgat gcagcaacat
     5881 ggcaacaata tatcgatgtg atgaacatct tgaaaaacgg gatgtttagc tacctagtta
     5941 tctatacagg tatcaatgct gcacaagttt ttggtgcaac accaactctt ggtggagtta
     6001 tcggggccgt tgtgatgctg acagggatga atccggaagc accactgaaa aacttattta
     6061 ctggtgaagc tcttgcagct ggacaaggtg ggatcctagg tgtcatcttc gctgtttggc
     6121 tattgtcaat cgttgagaaa aaactgcata aagtagtacc tgatgcggta gatatcatcg
     6181 ttacaccaac tctttcattg attgccatcg gtctgattga aattttcttc atcatgcctt
     6241 tagcaggttt catttctgat ggcatggtcg gtgggatcaa ctgggtactt ggcgttggtg
     6301 gagcatttgc cggcttcgtt ctaggcacat tattcttgcc gatggttatg ttcggactgc
     6361 atcaaatttt gactccaatc catgtgcaaa tgattgatga aactggtcgt acgctattgc
     6421 ttccaattct tgcgatggct ggtgcaggac aagtgggtgc agcattggca ttatggattc
     6481 gctgcaaaaa agataaagaa ttaacagaaa tgatcaaagg agctttacca gtcgggatcc
     6541 taggaatcgg cgaaccattg atttacggag taacattacc tctaggacgc ccatttatta
     6601 ctgcttgtat cggtggaggt atcggcgggg cagtgatcgg aatgatcgga aatgtcgggg
     6661 ctatcgctat tggaccttca ggagccgctt tgatcccatt gatctctgac ggtaaatggt
     6721 taggctacat ccttggatta ttggcagctt atgctggtgg cttcgttgct acattcttct
     6781 tcggtattcc taaagaccgt ttagaaaaag aagaattagc gactgaaaca gccacagtaa
     6841 atgaagtgcc tgaaaaagta gtacctcaaa cttcagctga aagtgtgact atcctctcat
     6901 cagttgctga cggaacagtt gtttctttag ataaagcaag tgatccagtt tttgcacaaa
     6961 agatgatggg tgaaggctac tttgtagaac cagaaaacgg acatatctat tctccagtta
     7021 ctggggtagt cagctctgtc ttcccaacga aacacgctat tgggatcaca acgccaaatg
     7081 gtttagaaat cttgcttcat atgggaatca acacagttga cttaggcggt acgccatttg
     7141 atgtgaaagt aacagaaggg cagcaagtga cacctgatac gcttgtagca gaagctgact
     7201 tagaagcgat caaagcagca ggcaaagaaa catccatgat cgtcttggta accaatatgg
     7261 atcgtgtaaa aaactttgtc ttggaaaaaa caggtaaagc aaaagcaaaa gcacctgtga
     7321 tggatgtcaa agcttaatat gatagaaaca atagaaacga aagaagaaac tgaaatctat
     7381 ttttcagctt cttctttttt attatttatt gttttctgct aaaatctgat aaattcgtgt
     7441 cacatcagat ggtgttcccc gtaattcata atctgccaag aaaggaaagc caaatgtttg
     7501 tgcatattgt ttggctgtta gacaatactg attattgaag tttttatttc cgctaccgac
     7561 tacaccaaga caaagtttat gattgtcttc atgttccaga tattcacgca tcgtttcggt
     7621 caagatttct gtatcaccgt tatctaaacc atttccgccg tctaaataag taggaacgaa
     7681 agtaaaaaat ggttctgttt ctttcgtaaa atcactattt tcatggattt cttttaaagt
     7741 aacttcagga ttgacaggat cttctgcatg cattttttca gcatattctg ctagatgttt
     7801 ggcaaaggca cgcgtatttc ctgaaatcga tatatataaa atgttcatgg atcatcctcc
     7861 aattctttcc ttttagtata gcatcttttt taagtttttt ggtggtgtaa tttggaaagg
     7921 gactaggcgc tttgcttgta taatgagaaa aaagccggta gaattatatt tctattaaag
     7981 tttttattaa ttaagtaaaa ataaaaaagg tttgttatac tttttgataa aagcggtttt
     8041 attttggagg atgcttacat ggatacagag caacagaaaa aaggaattgg tttaattcca
     8101 cttgctgcct tagtaattgg ttcagcgatt ggaggtggcg tatttggtat tatgacggac
     8161 atctccggat cggctggcgg cccagctatt ttcagttggg tactcgtagg aacagcaatg
     8221 ttgatgctct ccttgtcgat caataatatc aataaaaaac gtccagattt aaaaggcggg
     8281 atctttagtt acgcagaaga aggttttggc cgtttcgctg gttttatcag cggttggggt
     8341 tactggctca catgctggtt aggcaacgta gcttttgcaa cattgttgat gagcgcatta
     8401 ggatatttct tcccagtatt tgaaggtggg caaaatctgg catcgatcgt gatgagtaca
     8461 gtctttttat ggggatatgc atggctagtg aatcggggag tcgaaaatgc tagtttcgtc
     8521 aatgctatcg tcacattctg taaattagtg ccattatttt tatttattgt agtagctatc
     8581 agcacgtttt cgatccaaca attcattgat aacttttcta gcgacattat attgaatgcg
     8641 aatggacagc cgatcccgtt ttccagtcaa gtattgagtt gcatcatggt gatgctttgg
     8701 gtatttgttg gtattgaagg agcctctgtc gtaggttcac gagcaaaaaa acaatcagat
     8761 gttgggaaag caacgattct agggatattc ggcttgatta ttatttacgt cttggtttcc
     8821 atgcttccat atgggacaat gacgatgaat cagctgcaaa cgatcaaggc gcagcctgct
     8881 atgggctatg tgtttgaaat gatggttggt aaatggggag cagtcgtgat caacggcggt
     8941 ttgatcattt ctttagttgg tgtttggctc tcatggacga tcttaccgat tgaaacaatg
     9001 ggaaacatgg ctgaagacgg acttttgcct aaagcatggg gaaaactcaa caaaaatggg
     9061 gcaccaactt atgctatcgt attgacgacg ttatgtacga atgtcttttt actttcatta
     9121 ttattcactg acagtgctta taactttgct tacacacttg gtacagcagc tattttcttc
     9181 acgtggcttt tcttaggatt gtaccaaatg aaactttctt atcagcggaa agaatgggtc
     9241 caatttttag tagggctttt agcaagtgcc tttcaagtat gggctatgct agtcgtagcc
     9301 tttaatgaag taatgcttgt tcttgttctt ttcttgccag gcatttattt ttacctcaaa
     9361 gcacgtaagg agcaaaacga gcctgtacaa catatccact tagataagat tttacttata
     9421 ggtgtgacga tggtcggagt gattgcaatg gttttatttg ccactgggat gcttcacgta
     9481 tagaagtata aaagaagtat aaagagaggt tgtgagcctg tcgtttattc aaaaacatac
     9541 taggaaagga gctgtgacac aaatgttgtc acagctcctt tttcagaata aacggtgttt
     9601 aagagacagc caatcggcaa taaggctgaa cgtctctttc acagcctctt ttttgatgag
     9661 tcatcataca ttttttcgct taaatacgag atatcctaat gctaagaaca aaacgatgta
     9721 agctaaatta ccgaatagca tttgattagt agaaagatga gtcaattgct tcatgatctc
     9781 ttcgtttcca atttggtttt gcagattcaa catactgatc ggattccatt tgatccactc
     9841 ccatttttgg atgagtgcaa taaggatacc agatatgatt gacgaagcaa aataaaagac
     9901 gattcctgct gagatggatg cgccggaact attgataaag caagcaatca tcaacactaa
     9961 acttaaaatc agccataaac cgatataact acccaaagta taagtgaaca agctttggat
    10021 cagtgtttga ccagtagaaa ccttctccgt gaaggaaatc gaagggaaaa gaatcagttt
    10081 catcaacaca gtgacaataa tagctaaaag gtataaataa acagaataaa taactaatgt
    10141 gatccatttg cttactaata tttctcctcg agaatacttt cgataaagga gatttttcaa
    10201 tgtaccatat tgtgcttcca tcgaaatgat cgtgcttgca gctgcaatca tgataaagac
    10261 aatccaagat gtggcactaa aaagctgagc agtcatttca actggatcaa tgatcgtcgt
    10321 gtattttttg gctagaagtg ctgttcctat gagcaaaaca actaatagaa cagataaaac
    10381 agcagttgac tttttcttga tcaatttaaa taattcttgt tttacaagca atgtcatttt
    10441 tcttcttccc cctttactga atccagaatt tccaatagag aagattccaa atcattcgtc
    10501 agatgctgga cgtctgtcac atggatattt tgtgcagaca atagctggat cacggttcct
    10561 aataattctt cgttttcttt taaaagcttg attttatcag gatcttccaa agtatatcca
    10621 gcttctgtca agatcgttcg agcagctaga tcatttgttg tagagataac gatgaaatct
    10681 tgcgctgttt ggttcaattc agccattgta cattgtttga tgatctttcc ttgattgatc
    10741 aggatcagct catcgaccat tttttcaagc tcgctgagta agtgactgga gatcaagaaa
    10801 gaggtgccct gagtagccaa gtcggtaatg atttttcgca catctcttgt tgcttgtgga
    10861 tccaaaccgt tcatcggctc atctaaaata acgatagacg gatggttcag taaggcaaga
    10921 gcaattccca atttttgttt cattcccaac gagtaggatt tcgctttttt gtgaatgtac
    10981 ttttccatct tgagctgatt cacgatagtg tcgatagcag atacatcgtt ggtttcagag
    11041 aataacttca gatgatcata gcctgttaag aatggataga tacccgggta ttcgatcaat
    11101 gctcctactt gttccaaccc tttatgacta gagggagaaa tctcattttc accaatacag
    11161 attgttccat ctgagtaatg gatcagtcct aaaatagctt tcatgatcgt tgtttttcca
    11221 gcgccattag gacctactaa tccaacgatt ttgccagggg ataagctaaa actgacatcg
    11281 ctgagggctt gtttgtgacc aaagttcttg gataattctt ggaccgaaag acttttcaaa
    11341 aatattcact ccttttctat agatagtaca agcatacacc tgatgagtgg agaagtcgct
    11401 aggtcattgg acttatttct tattttttca taaaaaaatt gaatattaaa taaaaaggct
    11461 tattttttct gatcagtact tattccaagt gtctagtggc cgctagtaat cttgatgcca
    11521 atgatactga tgagtagcat ccccacaaag aaccaagtgg aaagattcag cttatcatga
    11581 aacaagacca caccaattat gaccgaacca actgcgccaa tccctgtcca gataggataa
    11641 gagagactca tcggcaaact tttgattgcc tttgacaaaa agtaaaaact agcaatcatg
    11701 ccaatcactg tgtaaaaaga ataattgatt ttagaaaaac catcactcca tttcatcatc
    11761 gttgaccaga atacttccaa aattcctgct atgattaatt ccatccatgc catacttaca
    11821 ttcctcctta actataaaaa aatacacaaa aagaggaaaa tcatcccttc aaagacctaa
    11881 tggaatgatc atcctcttta tcctacccgg tggcagattg tatatctttt tctgtagtat
    11941 agcacaccta tccagatatc ggcaatctca tactttttag taaagtgtac caagcactga
    12001 cggatatatc gttagcggaa aagaagatga atgctttgca atttcataga aagaagttgt
    12061 tatttattaa tcatttaaaa aaaacagtaa ctagacctta tttctatata ttatcgggca
    12121 ataaattaaa aaaagcttta ccttacaata agtaagataa tttttgtgat aaaaaaataa
    12181 cgaaaaatat ttaaaaatag tgtctatttc gtgagaaaac aactatattt tttatgtgtt
    12241 ttgctttata attaaaaaaa tattgtca
//
LOCUS       DACDYM010000267         6246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.106, whole genome shotgun sequence.
ACCESSION   DACDYM010000267 DACDYM010000000
VERSION     DACDYM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.106"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..521
                     /locus_tag="IWM59_002899"
     CDS             <1..521
                     /locus_tag="IWM59_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9980846.1"
                     /translation="QILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQ
                     EIRETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDS
                     LFTKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFA
                     FRAKSKKTSPIK"
     gene            681..2567
                     /locus_tag="IWM59_002900"
     CDS             681..2567
                     /locus_tag="IWM59_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9980847.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2579..3526
                     /locus_tag="IWM59_002901"
     CDS             2579..3526
                     /locus_tag="IWM59_002901"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9980848.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3546..4073
                     /locus_tag="IWM59_002902"
     CDS             3546..4073
                     /locus_tag="IWM59_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9980849.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4136..4789)
                     /locus_tag="IWM59_002903"
     CDS             complement(4136..4789)
                     /locus_tag="IWM59_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9980850.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4921..5763
                     /locus_tag="IWM59_002904"
     CDS             4921..5763
                     /locus_tag="IWM59_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9980851.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5921..>6246
                     /locus_tag="IWM59_002905"
     CDS             5921..>6246
                     /locus_tag="IWM59_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP9980852.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKR"
BASE COUNT         2239 a         1074 c         1294 g         1639 t
ORIGIN      
        1 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
       61 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
      121 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
      181 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
      241 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
      301 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
      361 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
      421 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
      481 cgtttcgagc aaagtcaaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
      541 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
      601 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
      661 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
      721 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
      781 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
      841 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
      901 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
      961 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     1021 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     1081 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     1141 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     1201 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     1261 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     1321 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     1381 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     1441 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     1501 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     1561 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     1621 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     1681 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     1741 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     1801 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     1861 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     1921 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     1981 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     2041 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     2101 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     2161 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     2221 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     2281 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     2341 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     2401 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     2461 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     2521 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     2581 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     2641 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     2701 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     2761 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     2821 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     2881 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     2941 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     3001 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     3061 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     3121 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     3181 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     3241 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     3301 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     3361 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     3421 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     3481 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     3541 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     3601 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     3661 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     3721 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     3781 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     3841 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     3901 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     3961 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     4021 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     4081 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     4141 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     4201 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     4261 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     4321 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     4381 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     4441 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     4501 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     4561 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     4621 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     4681 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     4741 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     4801 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     4861 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     4921 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     4981 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     5041 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     5101 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     5161 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     5221 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     5281 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     5341 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     5401 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     5461 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     5521 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     5581 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     5641 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     5701 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     5761 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     5821 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     5881 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     5941 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     6001 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     6061 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     6121 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     6181 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     6241 aaagcg
//
LOCUS       DACDYM010000268         6125 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.108, whole genome shotgun sequence.
ACCESSION   DACDYM010000268 DACDYM010000000
VERSION     DACDYM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.108"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWM59_002906"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWM59_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9980853.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IWM59_002907"
     CDS             2039..2227
                     /locus_tag="IWM59_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9980854.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="IWM59_002908"
     CDS             complement(2285..2638)
                     /locus_tag="IWM59_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9980855.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="IWM59_002909"
     CDS             3169..3591
                     /locus_tag="IWM59_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9980856.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="IWM59_002910"
     CDS             3588..3818
                     /locus_tag="IWM59_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9980857.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="IWM59_002911"
     CDS             4316..4516
                     /locus_tag="IWM59_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9980858.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..5736
                     /locus_tag="IWM59_002912"
     CDS             4543..5736
                     /locus_tag="IWM59_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9980859.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5804..>6125)
                     /locus_tag="IWM59_002913"
     CDS             complement(5804..>6125)
                     /locus_tag="IWM59_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9980860.1"
                     /translation="KLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGIRSVGVMGDG
                     RTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPAT
                     VEWE"
BASE COUNT         2096 a         1014 c         1172 g         1843 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
     5341 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
     5401 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
     5461 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
     5521 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
     5581 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
     5641 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
     5701 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
     5761 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
     5821 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     5881 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     5941 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     6001 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     6061 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     6121 gcttg
//
LOCUS       DACDYM010000269         6125 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.109, whole genome shotgun sequence.
ACCESSION   DACDYM010000269 DACDYM010000000
VERSION     DACDYM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.109"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWM59_002914"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWM59_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9980861.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IWM59_002915"
     CDS             2039..2227
                     /locus_tag="IWM59_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9980862.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="IWM59_002916"
     CDS             complement(2285..2638)
                     /locus_tag="IWM59_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9980863.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="IWM59_002917"
     CDS             3169..3591
                     /locus_tag="IWM59_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9980864.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="IWM59_002918"
     CDS             3588..3818
                     /locus_tag="IWM59_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9980865.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="IWM59_002919"
     CDS             4316..4516
                     /locus_tag="IWM59_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9980866.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..5736
                     /locus_tag="IWM59_002920"
     CDS             4543..5736
                     /locus_tag="IWM59_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9980867.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5804..>6125)
                     /locus_tag="IWM59_002921"
     CDS             complement(5804..>6125)
                     /locus_tag="IWM59_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9980868.1"
                     /translation="KLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGIRSVGVMGDG
                     RTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPAT
                     VEWE"
BASE COUNT         2095 a         1014 c         1173 g         1843 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
     5341 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
     5401 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
     5461 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
     5521 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
     5581 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
     5641 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
     5701 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
     5761 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
     5821 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     5881 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     5941 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     6001 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     6061 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     6121 gcttg
//
LOCUS       DACDYM010000270         5909 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.113, whole genome shotgun sequence.
ACCESSION   DACDYM010000270 DACDYM010000000
VERSION     DACDYM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.113"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..2048
                     /locus_tag="IWM59_002922"
     CDS             <1..2048
                     /locus_tag="IWM59_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9980869.1"
                     /translation="DIKQSQVDNNVGGGMIGAILYHFTYFLIAQPGSYLAAVLLLAGG
                     AFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAAKEERMKQRAEAKEARRLA
                     AKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPIDSFQENIHPANLE
                     KPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPTVDLLDSIPTVDQS
                     DEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVGVKVSKIVNLTDDI
                     ALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNHPDKLLEVPLGRDV
                     SGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEVKLMMIDPKMVELN
                     MYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNISGYNEFVQQKNLE
                     NGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILATQRPSVDV
                     ITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLPMGENKPIRVQGAF
                     ISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVVEMQTA
                     SISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            2278..3345
                     /gene="rlmN"
                     /locus_tag="IWM59_002923"
     CDS             2278..3345
                     /gene="rlmN"
                     /locus_tag="IWM59_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9980870.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3538..4641)
                     /locus_tag="IWM59_002924"
     CDS             complement(3538..4641)
                     /locus_tag="IWM59_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9980871.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5194..5742
                     /gene="aac(6')"
                     /locus_tag="IWM59_002925"
     CDS             5194..5742
                     /gene="aac(6')"
                     /locus_tag="IWM59_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9980872.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1971 a         1023 c         1277 g         1638 t
ORIGIN      
        1 gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc
       61 tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat
      121 tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta
      181 tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc
      241 gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc
      301 gcttagctgc aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg
      361 tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg
      421 agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag
      481 agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt
      541 tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag
      601 actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg
      661 agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg
      721 tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc
      781 cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt
      841 tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg
      901 aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa
      961 accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa
     1021 cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt
     1081 cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa
     1141 aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc
     1201 tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac
     1261 aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt
     1321 acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat
     1381 tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg
     1441 aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag
     1501 ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca
     1561 gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg
     1621 cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga
     1681 tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta
     1741 cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga
     1801 caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg
     1861 aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag
     1921 cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca
     1981 agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg
     2041 acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca
     2101 tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt
     2161 gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta
     2221 aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg
     2281 gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac
     2341 ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt
     2401 aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt
     2461 atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat
     2521 ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc
     2581 ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc
     2641 gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg
     2701 gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg
     2761 gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat
     2821 gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag tggattagca
     2881 cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac
     2941 gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag
     3001 aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa
     3061 tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta
     3121 ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa
     3181 catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag
     3241 aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc
     3301 ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt
     3361 tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa
     3421 gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa
     3481 gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta
     3541 actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag
     3601 aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt
     3661 tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt
     3721 taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa
     3781 ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta
     3841 tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt
     3901 atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac
     3961 ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata
     4021 agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc
     4081 tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat
     4141 acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg
     4201 tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac
     4261 acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt
     4321 agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa
     4381 caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt
     4441 tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt
     4501 tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc
     4561 atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg
     4621 taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt
     4681 ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga
     4741 taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga
     4801 acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac
     4861 cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga
     4921 aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat
     4981 catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt
     5041 gaataaagtg tttctgtata atttttttat tttataagta agataaataa tgaaagaata
     5101 gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac
     5161 tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat
     5221 aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat
     5281 ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca
     5341 gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca
     5401 ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact
     5461 cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta
     5521 ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca
     5581 tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa
     5641 ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt
     5701 tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca
     5761 gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt
     5821 gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtacca gaggttgtga
     5881 aaaagtcgct tagtttcgag tagtaagaa
//
LOCUS       DACDYM010000271         5129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.124, whole genome shotgun sequence.
ACCESSION   DACDYM010000271 DACDYM010000000
VERSION     DACDYM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.124"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..170)
                     /locus_tag="IWM59_002926"
     CDS             complement(<1..170)
                     /locus_tag="IWM59_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9980873.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESR"
     gene            322..1017
                     /gene="vanR"
                     /locus_tag="IWM59_002927"
     CDS             322..1017
                     /gene="vanR"
                     /locus_tag="IWM59_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9980874.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            995..2149
                     /locus_tag="IWM59_002928"
     CDS             995..2149
                     /locus_tag="IWM59_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9980875.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2364..3332
                     /locus_tag="IWM59_002929"
     CDS             2364..3332
                     /locus_tag="IWM59_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9980876.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3325..4356
                     /gene="vanA"
                     /locus_tag="IWM59_002930"
     CDS             3325..4356
                     /gene="vanA"
                     /locus_tag="IWM59_002930"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9980877.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4362..4970
                     /locus_tag="IWM59_002931"
     CDS             4362..4970
                     /locus_tag="IWM59_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9980878.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1576 a          971 c         1185 g         1397 t
ORIGIN      
        1 cgactttctg aacgttcata gtcatcggct tgaatatatt ctgttcgttg attttccatc
       61 aattggttga aaacagtggt taaaatattt ttagaaacat catcttttac cgaatgttca
      121 ataatacttt gaacctcttc gttgttcagt gtaaaatgta cttgggtcat gtaacgtcct
      181 cctgggtatg tttttgtggt taaaaacatt gtaccgtaaa agggctgtta catggccttt
      241 tatcttttac acaattatat ggactttatc ctataatctg ataaattatt aagggcaaac
      301 ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga tgaacatgaa
      361 attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt caaatactat
      421 accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc catattggac
      481 atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga caagcacacc
      541 tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac agggttaaca
      601 atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat tgctcgggta
      661 aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt
      721 atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct gaacgagaag
      781 cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga aaacaagggg
      841 aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata tttcagcaag
      901 agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt
      961 gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga aaaataaaaa
     1021 aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt
     1081 agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg gggattggat
     1141 cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga aattatatca
     1201 atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca ttagtattct
     1261 tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga taaataccgg
     1321 cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg aaatggatgt
     1381 tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc aggatgcaaa
     1441 gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc
     1501 ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca tgccggtaga
     1561 tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg aacagctaat
     1621 cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa caaaaacgca
     1681 catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc agctttccgc
     1741 acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg gcgaccctga
     1801 taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca gtgaggataa
     1861 cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa
     1921 cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct ataggctgga
     1981 caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg caaaagaaat
     2041 tattgttcag catggagggc agatttacgc ggaaagcaat gataactata cgacgtttag
     2101 ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag agatgtatat
     2161 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
     2221 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
     2281 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
     2341 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
     2401 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
     2461 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
     2521 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
     2581 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
     2641 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
     2701 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
     2761 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
     2821 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
     2881 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
     2941 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
     3001 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
     3061 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
     3121 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     3181 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     3241 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     3301 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     3361 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     3421 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     3481 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     3541 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     3601 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     3661 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     3721 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     3781 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     3841 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     3901 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     3961 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg cgaggtggac
     4021 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     4081 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     4141 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     4201 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     4261 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     4321 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     4381 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     4441 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     4501 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     4561 gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     4621 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     4681 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     4741 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     4801 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     4861 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     4921 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     4981 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     5041 gttcttaggg aatttatata tagtagatag tattgaagat gtaaggcaga gggttctgtt
     5101 gcaaagtttt aaataaagaa taaaatccc
//
LOCUS       DACDYM010000272         3386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.152, whole genome shotgun sequence.
ACCESSION   DACDYM010000272 DACDYM010000000
VERSION     DACDYM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.152"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            405..869
                     /locus_tag="IWM59_002932"
     CDS             405..869
                     /locus_tag="IWM59_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9980879.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            853..1482
                     /locus_tag="IWM59_002933"
     CDS             853..1482
                     /locus_tag="IWM59_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9980880.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1836..3314
                     /gene="msr"
                     /locus_tag="IWM59_002934"
     CDS             1836..3314
                     /gene="msr"
                     /locus_tag="IWM59_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9980881.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT         1170 a          557 c          690 g          969 t
ORIGIN      
        1 gaacttatta ccgcagatgc aagtttttga acaccgttta ctcaaaaaag gttgtgacaa
       61 catcttgtca taactttttt attactgttt aagcatactg atccaatttc taatattcga
      121 cgggattctt gcaaaaggcg atttcggaaa tttttggcgg tgtaaaattt tctactgcag
      181 aagagctatc ttctatagtt tgataagaat caataatctt ggttttagcg ttgtgctttc
      241 tttatcattt tttccgttca ttctacaaaa aatttctttt ctctatactt ttctattgag
      301 agcgtttttt ttattacaca gctgattctt tgactttttt ctttttttat tctattattg
      361 acaagtcaat cgttgacgag tcaattatta gaaaggagga aattatgtct ttagaagaga
      421 ttagtcacct tttatatcaa atcaaattag ccgatcaaaa aatcgtccag atctttgaag
      481 aaaatatcgg tataagtctt actagatacg agatcatgat gagactgaaa gaagaaaatc
      541 cattgctaca aagcagtttg caagaaaagt tgcagattga tcaaggagca gttactcgtc
      601 atctgaaggt attggaagca aatggctatg tttcgcgtca gagaaatcct cagaacaacc
      661 gggaagtgta tgtagcgcta acagaaaaag cccagaagaa aatcgagcat tgtgaaatgg
      721 accataaaga tacaggttcc gtgctatccc cggctttcag cgatgtagaa atgaaacaat
      781 ttctaacact tttagataaa ttgaataccc aactttgcaa tataacaaca gaaacgaaag
      841 aggttaataa taatggaaca aaaaaatgat ttttcaacaa ttatgactgg tagaagatcc
      901 gttcgcgtat atgatgaaac atacaaaatc ccacacgaag aaatgctgga aatgatcaaa
      961 gaagcgacga ctgcaccttc ttctgtcaat atgcaaccat ggcgctttgt ggtcgctgaa
     1021 agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat
     1081 acttcttctg caatggtcat gatttttggc gatatgcaat gttacgaata tggcgaagaa
     1141 atctataacc aagcttacga atcagggaag atgccaaaag aagtcagaga tcaacaatta
     1201 gctgcgatca ttccttatta caagagtttt tcaagagaag aaatgaacga tgtagtcaaa
     1261 gtcgattcta gcttagcggc tatgcaattc atgttagttg cgcgtgatca tggctatgaa
     1321 acaaatccaa ttggtggatt cgaagcagat cagttggcag aggcatttgg tttggataaa
     1381 gaccgttatg ttccagttat tattttatca ataggaaaag cggtagaaga aggttacgag
     1441 tctgttcgtt tagcaccaga aaagatcact acgttcgaat aaaaggtttt cagttactat
     1501 tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa
     1561 aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa ataaattcaa
     1621 aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg aacgacagta
     1681 gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg ttcagagtag
     1741 gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt agtatcgata
     1801 attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa
     1861 caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct
     1921 atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca
     1981 aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca
     2041 actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca
     2101 gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac
     2161 gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc
     2221 caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt
     2281 atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa
     2341 tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca
     2401 ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga
     2461 aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag
     2521 tgtccagtaa aaagaaaaag caacaaatca gaccggatcg gttgtcttcc tctaaacaaa
     2581 aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa
     2641 aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat
     2701 ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg
     2761 ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg
     2821 tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag
     2881 gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga
     2941 catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg
     3001 tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta
     3061 cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta
     3121 atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag
     3181 agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg
     3241 tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta
     3301 cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga
     3361 ttgcctacaa ataaaaaaga aggaat
//
LOCUS       DACDYM010000273         3277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.153, whole genome shotgun sequence.
ACCESSION   DACDYM010000273 DACDYM010000000
VERSION     DACDYM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.153"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            275..772
                     /gene="dfrG"
                     /locus_tag="IWM59_002935"
     CDS             275..772
                     /gene="dfrG"
                     /locus_tag="IWM59_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9980882.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(844..2796)
                     /locus_tag="IWM59_002936"
     CDS             complement(844..2796)
                     /locus_tag="IWM59_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9980883.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2803..3099)
                     /locus_tag="IWM59_002937"
     CDS             complement(2803..3099)
                     /locus_tag="IWM59_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980884.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1016 a          566 c          538 g         1157 t
ORIGIN      
        1 taagtagttc agttttggag tacaaaatga caaaatgtac ttcaaaatgg actacttttt
       61 tcattttaac ttacatacca aattacatac aaaatgattt ataaaatgca attgaaaatg
      121 ataattgaat gaaaatttgt gattttgtgg tattatgata atataactta tagaaaaaat
      181 tgcttattca actaacgggc aggataatga gaaaaggttt tttgatagaa ataagaaatt
      241 agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat
      301 ggataagaat agagtgattg gcaaagagaa tgacattcct tggaggattc ccaaggactg
      361 ggaatatgtt aaaaatacta caaagggaca tccgataata ttaggtagga agaaccttga
      421 atcaatcgga agagccttac ctgacagaag aaatattatt ctgacgagag ataaggggtt
      481 tacctttaat ggttgtgaaa ttgttcattc aatagaagat gtttttgagt tatgtaaaaa
      541 cgaagaagaa atttttattt tcggaggaga acagatttat aatttgtttt tcccttatgt
      601 tgagaaaatg tacatcacaa aaatacatca tgaattcgaa ggagatactt tttttccaga
      661 agtgaattat gaggaatgga atgaggtatt tgcccaaaaa gggataaaga atgataaaaa
      721 tccgtataac tactattttc atgtatatga aagaaaaaac ttattgagtt aacgggtgcg
      781 ttaattgaag aagaacagta aagataaagc acaagagctt gggattactc caagctcttt
      841 ttgtcatttg aaataaatta tatctttcca atcaaattgt ttatctgtaa tccctaatct
      901 ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa gggcgacaaa agttgtagta
      961 agttcttaga attgtaatcg caaattgagc atacttcgga ttgaagttag cgtaaatata
     1021 gctcttctta tcaccacgag ctgttgttaa tggtctttca agagatgata tataacgtct
     1081 tatctgttga ataaagctat tggtcgaatg gtcattaaca tttaatagca tctttgcgat
     1141 ttcgtttggt tccagagcag ataaatccgt tctacaatca actgaataaa atcctttatc
     1201 cttagtggct aatggatgtt tgatagggtt atcggcatat ttgcgatatc tttctccatc
     1261 tttactaata gcctcttcgt ggaattgatg acttgttagt aattcagtta gataatgaga
     1321 tgccaaagtc cgtagaaact ttgttttgaa tcctctgata tctccccaat cgagcagttc
     1381 tgcttttgct tgtccgaatt cttttaagca ttgttttctg cttttcgtct tgttaacttg
     1441 gcaaataaaa tgatgtgcat cgctcaacct taattctttc gtgaatactc tataaaaggc
     1501 gtttctaata gagaagtcat catcactaat cattcgccat tcgtcggcat taaccatttg
     1561 tttaattaac caatagtgag cagttgtagt ataaggagca tcaacattta aaccatcgac
     1621 aaatctactc ctattagtaa ttttccctaa ttcgtgaaga taagcattcc tattttcgct
     1681 atcgttagcg gatggttctt gtggataata agaccaatct aacctatcat ttttacgact
     1741 aaaagtattg agatggtctt ctttgaattt tcttgtatct tcatttaatt catccattga
     1801 aatattccaa tcataggcaa catcagaacg gaacacatat cttgataaga cctcagcaga
     1861 tacaactata tacgtttgca tattaaggtc ttcaaacccc gtatatttct ttgaaccttg
     1921 tcctttctta cggacgttat ttaggtagta gtgcatcttg tccgtattaa gccacatctc
     1981 gttaaatttc tttgtttgaa ggggttgtgt ctcatatcgt tctaaaaact ccaaacaacg
     2041 cctatacagc cactccagct tatgatagta ggttccaact ccaattccta aaatatcaca
     2101 agtacgactg actgaaaccc ttccaacaac catcttagcg aacattggaa gaatggtgtt
     2161 cttttgttgg tgataagtag tggtttcttc tctttttggt agaacattag tgaacttctt
     2221 acacgcctta cattgatagc gttgagattt gcccctactt ttcccccttt tgtaaaattg
     2281 cttcggctca ttgaatgggg ttgattcttc ttccgagcag ccttctttat gaaattgata
     2341 atctggctca acatcttgga tgctattgat ttctataagg cgtttaattt cttcaacaac
     2401 agaccaatta gataaagggg ttactgtaca gttttgcgat acacctctgc caataggatt
     2461 gtcattgcag tacatacctt tatgaccttt atctttggaa ctaccagtca ttttatatcg
     2521 acttggtttc ccttttacat ctttgaattt gtgctgttct ttcccaaaat tacaacaaaa
     2581 cggattgatg cagtaattat attgaatggg atattcagaa ccgttccatt taaaagacgt
     2641 tggcaagaac aacattttgt tataccttgt tgcaaatcgt tttggagtta ataaatcata
     2701 ttgtttagcc ctatctttta attcttcgtt tgaaatcgga attttaacat ctacaatttc
     2761 atcttccttc gtggctaatc ttttaagctt ggtcattgtc tatcaaccca ctctatcttt
     2821 gatacccatt ttttcaacaa tccgcttatt acttcgcttg ttggcaatcc attcaataaa
     2881 cttcttatcg tctaaaatgt tcaataagaa ttcatccact gcttcgcttt cagtacgctc
     2941 tgcatattca gcgtattgct taactatcac ccttacttgc tctgatattt cccaatccac
     3001 agacttagcg ttcttatttt ttggctcaag aaattgcata ggaaaatgca cccctttttg
     3061 cttagtaaat tgtaaccatt ttaacacacc aatttgcatt cctacaataa ttatttcacg
     3121 ttcgcatttt tatatacatt ttaacttaca ttttgtggtg taaattaaca acaaacgttg
     3181 atttaacaat aaaaaacgag tatcaaattc agttgcaatt tgatactcat ttaggtttac
     3241 attttcactt tttgtactcc aaaactgaac tacttaa
//
LOCUS       DACDYM010000274         2861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.162, whole genome shotgun sequence.
ACCESSION   DACDYM010000274 DACDYM010000000
VERSION     DACDYM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.162"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWM59_002938"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWM59_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9980885.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>2861
                     /locus_tag="IWM59_002939"
     CDS             2039..>2861
                     /locus_tag="IWM59_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9980886.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGF"
BASE COUNT          937 a          434 c          589 g          901 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaagaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt g
//
LOCUS       DACDYM010000275         2861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.163, whole genome shotgun sequence.
ACCESSION   DACDYM010000275 DACDYM010000000
VERSION     DACDYM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.163"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWM59_002940"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWM59_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9980887.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..>2861
                     /locus_tag="IWM59_002941"
     CDS             2039..>2861
                     /locus_tag="IWM59_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9980888.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGF"
BASE COUNT          936 a          434 c          590 g          901 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaagaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt g
//
LOCUS       DACDYM010000276         2009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.174, whole genome shotgun sequence.
ACCESSION   DACDYM010000276 DACDYM010000000
VERSION     DACDYM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.174"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..331
                     /locus_tag="IWM59_002942"
     CDS             <1..331
                     /locus_tag="IWM59_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980889.1"
                     /translation="AGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNE
                     VILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVR
                     YMFNKIT"
     gene            429..1802
                     /gene="tet(L)"
                     /locus_tag="IWM59_002943"
     CDS             429..1802
                     /gene="tet(L)"
                     /locus_tag="IWM59_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP9980890.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT          591 a          299 c          396 g          723 t
ORIGIN      
        1 agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata
       61 tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt
      121 gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg
      181 tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta
      241 ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa
      301 agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt
      361 ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg
      421 aattaattat gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc
      481 tttgcgtttt atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg
      541 atattgcaaa tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta
      601 tgttaacctt ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca
      661 aaaggttact cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg
      721 gccattcttt cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg
      781 catttccagc actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta
      841 aagcatttgg tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg
      901 gtggaatgat agcccattat attcattggt cctatcttct actcattcct atgataacaa
      961 ttatcactgt tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt
     1021 ttgatatcaa aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa
     1081 catcatatag catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac
     1141 atatcaagaa agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta
     1201 tgattggagt tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg
     1261 ttccttatat gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta
     1321 ttttccctgg aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata
     1381 gaagaggtcc tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa
     1441 ctgcttcctt tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt
     1501 taggtgggct ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac
     1561 agcaggaagc tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa
     1621 caggtattgc aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac
     1681 ctatggaagt tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa
     1741 tcattgtcat tagttggctg gttaccttga atgtatataa acattctcaa agggatttct
     1801 aaatcgttaa gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg
     1861 aattcaaatc tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc
     1921 tctcttgtat cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca
     1981 ataaaaattt tattcttgct gagtctggc
//
LOCUS       DACDYM010000277         1781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.181, whole genome shotgun sequence.
ACCESSION   DACDYM010000277 DACDYM010000000
VERSION     DACDYM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.181"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..416
                     /locus_tag="IWM59_002944"
     CDS             24..416
                     /locus_tag="IWM59_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9980891.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IWM59_002945"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IWM59_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP9980892.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DACDYM010000278         1533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.187, whole genome shotgun sequence.
ACCESSION   DACDYM010000278 DACDYM010000000
VERSION     DACDYM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.187"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            228..1103
                     /locus_tag="IWM59_002946"
                     /note="aph(2'')-Ia"
     CDS             228..1103
                     /locus_tag="IWM59_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP9980893.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1233..>1533)
                     /locus_tag="IWM59_002947"
     CDS             complement(1233..>1533)
                     /locus_tag="IWM59_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9980894.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          610 a          144 c          260 g          519 t
ORIGIN      
        1 gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag
       61 ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt
      121 ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt
      181 atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa
      241 ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg
      301 atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata
      361 agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa
      421 ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag
      481 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
      541 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      601 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      661 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      721 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      781 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      841 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      901 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      961 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
     1021 ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt
     1081 ataaaaggac ttataaagat tgattatata atatatgaaa agctattata aaagacatta
     1141 gtattaaata gtttaaaaaa atgaaaaata ataaaggaag tgagtcaatt ccagactcct
     1201 gtgtaaaatg ctatacaatg tttttaccat ttctacttat caaaattgat gtattttctt
     1261 gaagaataaa tccattcatc atgtaggtcc ataagaacgg ctccaattaa gcgattggct
     1321 gatgtttgat tggggaagat gcgaataatc ttttctcttc tgcgtacttc ttgattcagt
     1381 cgttcaatta gattggtact ctttagtcga ttgtgggaat ttccttgtac ggtatattga
     1441 aaggcgtctt cgaatccatc atccaatgat gcgcaagctt ttgaatattt tggttggttc
     1501 tgttgcaaag ttttaaatct actatcaaat aag
//
LOCUS       DACDYM010000279         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.189, whole genome shotgun sequence.
ACCESSION   DACDYM010000279 DACDYM010000000
VERSION     DACDYM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.189"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWM59_002948"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWM59_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWM59_002949"
     CDS             394..477
                     /locus_tag="IWM59_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9980895.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWM59_002950"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWM59_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9980896.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACDYM010000280         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.202, whole genome shotgun sequence.
ACCESSION   DACDYM010000280 DACDYM010000000
VERSION     DACDYM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.202"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWM59_002951"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWM59_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWM59_002952"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWM59_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9980897.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDYM010000281         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.216, whole genome shotgun sequence.
ACCESSION   DACDYM010000281 DACDYM010000000
VERSION     DACDYM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.216"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            259..>1195
                     /gene="vanY"
                     /locus_tag="IWM59_002953"
     CDS             259..>1195
                     /gene="vanY"
                     /locus_tag="IWM59_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9980898.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          427 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaata
//
LOCUS       DACDYM010000282          968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31914_2017
            SAMN12639972-rid9881193.guided.229, whole genome shotgun sequence.
ACCESSION   DACDYM010000282 DACDYM010000000
VERSION     DACDYM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639972
            Sequence Read Archive: SRR10023362
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741887.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741887.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:53:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 35 of 64
            Pseudo Genes (incomplete)         :: 22 of 64
            Pseudo Genes (internal stop)      :: 15 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639972-rid9881193.guided.229"
                     /isolate="E31914_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            85..144
                     /gene="ermCL"
                     /locus_tag="IWM59_002954"
     CDS             85..144
                     /gene="ermCL"
                     /locus_tag="IWM59_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAP9980899.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            194..928
                     /locus_tag="IWM59_002955"
     CDS             194..928
                     /locus_tag="IWM59_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAP9980900.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          402 a          118 c          134 g          314 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccagc ttgtgctata attattctaa
       61 tttcataaaa aggagaatta atatatgggc atttttagta tttttgtaat caacacagtt
      121 cattatcaac caaacaaaaa ataagtggtt ataatggatt gttaatataa aaacctcata
      181 ggagtgggtt ataatgaaca aaaaaaatat aaaagatagt caaaacttta ttacttcaaa
      241 gcatcatata aatgaaattt tgagaaatgt acatttaaat acaaatgata atattattga
      301 gattggttca gggaaaggtc atttcacgtt tgaattagct aaaaggtgta attatgtaac
      361 cgccattgaa atagatccta aattatgtag gataactaaa aacaaactta ttgaatatga
      421 gaacttccag gttatcaata aagatatttt acaatttaag tttcctaaaa ataagtcata
      481 taagatatat ggaaatatac cctacaatat aagtacagat ataattcgaa aaattgtttt
      541 tgaaagcaca gctacagaaa gttatttaat agtggaatat ggatttgcta aaaggttgct
      601 aaatacaaat cgttcactag cactattttt aatgacagaa gttgatatat ccatattaag
      661 taaaatccct agagaatact ttcatccaaa acctagagtt aatagctcgt taattgtatt
      721 aaaaagacac ccttcaaaaa tatcactcaa agatagaaaa caatatgaaa attttgttat
      781 gaaatgggtt aacaaagaat acataaaact attttccaaa aaccaatttt atcaagcctt
      841 aaaatatgca agaattgacg atttaaacaa tattagcttt gaacaattct tgtctctttt
      901 caatagctat aaattattta atagataagg gctctttgtc aaataggact gatagagtga
      961 gataagaa
//
